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Gene Dmel\CkIIα

General Information
Symbol	Dmel\CkIIα	Species	D. melanogaster
Name	Casein kinase II α subunit	Annotation symbol	CG17520
Feature type	protein_coding_gene	FlyBase ID	FBgn0000258
Gene Model Status	Current	Stock availability	7 publicly available
Also Known As	CKII, CK2, CK2α, Tik, CKIIalpha, DmCKIIα, dCK2α, DmCK2α
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	80D1-80D1	Sequence location	3L:23,092,680..23,096,562 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Casein kinase II α subunit is referred to in FlyBase by the symbol Dmel\CkIIα (CG17520, FBgn0000258). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; protein serine/threonine kinase activity. There is experimental evidence for 13 unique biological process terms, many of which group under: biological regulation; anatomical structure development; cellular component organization or biogenesis; rhythmic process; regulation of metabolic process; sensory organ development; circadian rhythm; regulation of biological process; cell-cell signaling; macromolecule modification; locomotory behavior. 16 alleles are reported. The phenotypes of these alleles are annotated with: eye; trichogen cell; microchaeta; interommatidial bristle; ommatidium; macrochaeta. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine-protein kinase, active site; Serine/threonine-protein kinase, catalytic domain; Serine/threonine-protein kinase-like domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed within 00-18 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1640051_s_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of CkIIalpha. Gene sequence location is 3L:23092680..23096562.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	CkII α: Casein kinase II, α subunit The structural gene for the catalytic subunit of casein kinase II (CKII). CKII is a cyclic-nucleotide-independent, Ca2+ and calmodulin-insensitive protein kinase. It phosphorylates serine and threonine residues of a broad range of nuclear and non-nuclear proteins with functions in development, cell division, and oncogenesis. It exists as a 130,000 molecular weight α2β2 tetramer. Purification of the Drosophila enzyme described by Glover, Shelton, and Brutlag (1983, J. Biol. Chem. 258: 3258-65).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Controlled Vocabulary Terms negative regulation of protein ubiquitination positive regulation of hh target transcription factor activity regulation of protein stability positive regulation of smoothened signaling pathway Alleles CkIIalpha[KM.Scer\UAS.T:Zzzz\FLAG] CkIIalpha[Scer\UAS.T:Zzzz\FLAG]
FB2012_01	Sequence features BKN28436 BKN50525 BKN50398 HFA11946 References Guenin et al., 2010, BMC Genomics 11: 47 Wong et al., 2011, PLoS ONE 6(9): e24355 Andersen et al., 2010, Mol. Cell. Biol. 30(19): 4744--4755
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 80D1-80D1   Limits computationally determined from genome sequence between P{lacW}l(3)j1B10j1B10 and P{lacW}l(3)j2C4j2C4
Experimentally Determined Cytological Location
Cytogenetic map Notes References 80A-80A   (determined by in situ hybridisation)   (Saxena et al., 1987)
Experimentally Determined Recombination Data
Location
Left of (cM)	ry P{EP}CG1090EP3028 l(3)L1233 Ki (Lin et al., 2002)
Right of (cM)	cyc kni Ten-m l(3)s2253 nrm (Lin et al., 2002)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CkIIα for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CkIIalpha-RA FBtr0070042  NM_168985   1598   336 CkIIα-RB FBtr0070043  NM_080179   1621   336 CkIIα-RC FBtr0070044  NM_168986   1485   336
Additional Transcript Data & Comments
Reported size (kB)	1.467 (longest cDNA) (Saxena et al., 1987)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CkIIalpha-PA   FBpp0070041   40.0   336   7.26     NP_730774   AAN11415 CkIIalpha-PB   FBpp0070042   40.0   336   7.26     NP_524918   AAF45439 CkIIα-PC   FBpp0070043   40.0   336   7.26     NP_730775   AAN11416
Additional Polypeptide Data & Comments
Reported size (kDa)	336 (aa); 39.833 (kD predicted) (Saxena et al., 1987) 36.7 (kD observed) (Glover et al., 1983)
Comments	Purified CkII protein kinases were able to phosphorylate acid-soluble proteins from Drosophila Kc cells. (Dahmus et al., 1984)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine-protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) Serine/threonine-protein kinase-like domain (IPR017442)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA392271   AA695981   AA802813   AA816244   AA948982   AE014296 AAF45439   AE014296 AAN11415   AE014296 AAN11416   AI259010   AI261006   AW942772   AX664479   AY069553 AAL39698   BF500464   BF505811   BI141934   BI164386   BI164444   BI170007   BI215307   BI234102   BI237173   BI362115   BI581678   BI611906   BP544476   BP544477   BP554686   BQ102770   BQ103128   BQ103410   BQ103631   CA806263   CO154098   CO183602   CO267452   CO298297   CO303259   CO304752   CO305011   CO306162   CO306553   CO306575   CO307154   CO325705   CO329963   EC055454   EC063422   EC065790   EC066021   EC069238   EC077067   EC077204   EC088922   EC204903   EC214530   EC214868   EC222050   EC248775   EV581602   EV584850   EV595714   GH941413   GH941620   GH941759   GH941846   GH944063   GH945991   GH946140   GH946279   GH946835   GH947009   GH947737   GH948246   GH948988   GH949443   GH950069   GH950243   GH951338   GH951837   GH953086   GH953287   GH953585   GH953722   GH953862   GH953865   GH954222   GH954914   GH955580   GH955852   GH956249   GH956350   GH956570   GH957273   GH957440   GH957729   GH958504   GH958551   GH959052   GH959243   GH959668   GH960555   GH960959   GH964397   GH964963   GH965063   GH965447   GH965670   GH965814   GH965829   GH966341   GH967661   GH969068   GH969800   GH969921   GH970243   GH970378   GH970434   GH970967   GH971640   GH971886   GH971946   GH972831   GH973006   GH973130   GH973730   GH973780   GH973990   GH974175   GH974570   GH975238   GH975425   GH975838   GH976095   GH977517   GH978304   GH979082   GH981148   GH981287   GH981426   GH981525   GH984450   GH984881   M16534 AAA28429
UniProtKB/Swiss-Prot	P08181
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010) day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0000258    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0000258 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed within 00-18 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0000258 expression level Developmental Stage   Expression Level embryo 00-02hr    9990 embryo 02-04hr    4543 embryo 04-06hr    6388 embryo 06-08hr    5976 embryo 08-10hr    7012 embryo 10-12hr    6215 embryo 12-14hr    9262 embryo 14-16hr    2403 embryo 16-18hr    3404 embryo 18-20hr    1758 embryo 20-22hr    1746 embryo 22-24hr    2907 larva L1    3337 larva L2    4839 larva L3 12hr old    3497 larva L3 puffstage 1-2    3021 larva L3 puffstage 3-6    3862 larva L3 puffstage 7-9    6767 white prepupae new    5690 white prepupae 12hr    5216 white prepupae 24hr    5504 pupae 2d postWPP    5525 pupae 3d postWPP    3944 pupae 4d postWPP    1507 adult male 01day    2668 adult male 05day    1999 adult male 30day    1630 adult female 01day    3146 adult female 05day    9641 adult female 30day    7039 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (9990) embryo 02-04hr  (4543) embryo 04-06hr  (6388) embryo 06-08hr  (5976) embryo 08-10hr  (7012) embryo 10-12hr  (6215) embryo 12-14hr  (9262) embryo 14-16hr  (2403) embryo 16-18hr  (3404) embryo 18-20hr  (1758) embryo 20-22hr  (1746) embryo 22-24hr  (2907) larva L1  (3337) larva L2  (4839) larva L3 12hr old  (3497) larva L3 puffstage 1-2  (3021) larva L3 puffstage 3-6  (3862) larva L3 puffstage 7-9  (6767) white prepupae new  (5690) white prepupae 12hr  (5216) white prepupae 24hr  (5504) pupae 2d postWPP  (5525) pupae 3d postWPP  (3944) pupae 4d postWPP  (1507) adult male 01day  (2668) adult male 05day  (1999) adult male 30day  (1630) adult female 01day  (3146) adult female 05day  (9641) adult female 30day  (7039) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (9990) embryo 02-04hr  (4543) embryo 04-06hr  (6388) embryo 06-08hr  (5976) embryo 08-10hr  (7012) embryo 10-12hr  (6215) embryo 12-14hr  (9262) embryo 14-16hr  (2403) embryo 16-18hr  (3404) embryo 18-20hr  (1758) embryo 20-22hr  (1746) embryo 22-24hr  (2907) larva L1  (3337) larva L2  (4839) larva L3 12hr old  (3497) larva L3 puffstage 1-2  (3021) larva L3 puffstage 3-6  (3862) larva L3 puffstage 7-9  (6767) white prepupae new  (5690) white prepupae 12hr  (5216) white prepupae 24hr  (5504) pupae 2d postWPP  (5525) pupae 3d postWPP  (3944) pupae 4d postWPP  1507 adult male 01day  (2668) adult male 05day  (1999) adult male 30day  (1630) adult female 01day  (3146) adult female 05day  (9641) adult female 30day  (7039) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    9990 embryo 02-04hr    4543 embryo 04-06hr    6388 embryo 06-08hr    5976 embryo 08-10hr    7012 embryo 10-12hr    6215 embryo 12-14hr    9262 embryo 14-16hr    2403 embryo 16-18hr    3404 embryo 18-20hr    1758 embryo 20-22hr    1746 embryo 22-24hr    2907 larva L1    3337 larva L2    4839 larva L3 12hr old    3497 larva L3 puffstage 1-2    3021 larva L3 puffstage 3-6    3862 larva L3 puffstage 7-9    6767 white prepupae new    5690 white prepupae 12hr    5216 white prepupae 24hr    5504 pupae 2d postWPP    5525 pupae 3d postWPP    3944 pupae 4d postWPP    1507 adult male 01day    2668 adult male 05day    1999 adult male 30day    1630 adult female 01day    3146 adult female 05day    9641 adult female 30day    7039 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    9990 embryo 02-04hr    4543 embryo 04-06hr    6388 embryo 06-08hr    5976 embryo 08-10hr    7012 embryo 10-12hr    6215 embryo 12-14hr    9262 embryo 14-16hr    2403 embryo 16-18hr    3404 embryo 18-20hr    1758 embryo 20-22hr    1746 embryo 22-24hr    2907 larva L1    3337 larva L2    4839 larva L3 12hr old    3497 larva L3 puffstage 1-2    3021 larva L3 puffstage 3-6    3862 larva L3 puffstage 7-9    6767 white prepupae new    5690 white prepupae 12hr    5216 white prepupae 24hr    5504 pupae 2d postWPP    5525 pupae 3d postWPP    3944 pupae 4d postWPP    1507 adult male 01day    2668 adult male 05day    1999 adult male 30day    1630 adult female 01day    3146 adult female 05day    9641 adult female 30day    7039 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0000258 expression level Developmental Stage   Expression Level embryo 00-02hr    9990 embryo 02-04hr    4543 embryo 04-06hr    6388 embryo 06-08hr    5976 embryo 08-10hr    7012 embryo 10-12hr    6215 embryo 12-14hr    9262 embryo 14-16hr    2403 embryo 16-18hr    3404 embryo 18-20hr    1758 embryo 20-22hr    1746 embryo 22-24hr    2907 larva L1    3337 larva L2    4839 larva L3 12hr old    3497 larva L3 puffstage 1-2    3021 larva L3 puffstage 3-6    3862 larva L3 puffstage 7-9    6767 white prepupae new    5690 white prepupae 12hr    5216 white prepupae 24hr    5504 pupae 2d postWPP    5525 pupae 3d postWPP    3944 pupae 4d postWPP    1507 adult male 01day    2668 adult male 05day    1999 adult male 30day    1630 adult female 01day    3146 adult female 05day    9641 adult female 30day    7039 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (9990) embryo 02-04hr  (4543) embryo 04-06hr  (6388) embryo 06-08hr  (5976) embryo 08-10hr  (7012) embryo 10-12hr  (6215) embryo 12-14hr  (9262) embryo 14-16hr  (2403) embryo 16-18hr  (3404) embryo 18-20hr  (1758) embryo 20-22hr  (1746) embryo 22-24hr  (2907) larva L1  (3337) larva L2  (4839) larva L3 12hr old  (3497) larva L3 puffstage 1-2  (3021) larva L3 puffstage 3-6  (3862) larva L3 puffstage 7-9  (6767) white prepupae new  (5690) white prepupae 12hr  (5216) white prepupae 24hr  (5504) pupae 2d postWPP  (5525) pupae 3d postWPP  (3944) pupae 4d postWPP  (1507) adult male 01day  (2668) adult male 05day  (1999) adult male 30day  (1630) adult female 01day  (3146) adult female 05day  (9641) adult female 30day  (7039) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (9990) embryo 02-04hr  (4543) embryo 04-06hr  (6388) embryo 06-08hr  (5976) embryo 08-10hr  (7012) embryo 10-12hr  (6215) embryo 12-14hr  (9262) embryo 14-16hr  2403 embryo 16-18hr  (3404) embryo 18-20hr  1758 embryo 20-22hr  1746 embryo 22-24hr  (2907) larva L1  (3337) larva L2  (4839) larva L3 12hr old  (3497) larva L3 puffstage 1-2  (3021) larva L3 puffstage 3-6  (3862) larva L3 puffstage 7-9  (6767) white prepupae new  (5690) white prepupae 12hr  (5216) white prepupae 24hr  (5504) pupae 2d postWPP  (5525) pupae 3d postWPP  (3944) pupae 4d postWPP  1507 adult male 01day  (2668) adult male 05day  1999 adult male 30day  1630 adult female 01day  (3146) adult female 05day  (9641) adult female 30day  (7039) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    9990 embryo 02-04hr    4543 embryo 04-06hr    6388 embryo 06-08hr    5976 embryo 08-10hr    7012 embryo 10-12hr    6215 embryo 12-14hr    9262 embryo 14-16hr    2403 embryo 16-18hr    3404 embryo 18-20hr    1758 embryo 20-22hr    1746 embryo 22-24hr    2907 larva L1    3337 larva L2    4839 larva L3 12hr old    3497 larva L3 puffstage 1-2    3021 larva L3 puffstage 3-6    3862 larva L3 puffstage 7-9    6767 white prepupae new    5690 white prepupae 12hr    5216 white prepupae 24hr    5504 pupae 2d postWPP    5525 pupae 3d postWPP    3944 pupae 4d postWPP    1507 adult male 01day    2668 adult male 05day    1999 adult male 30day    1630 adult female 01day    3146 adult female 05day    9641 adult female 30day    7039 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    9990 embryo 02-04hr    4543 embryo 04-06hr    6388 embryo 06-08hr    5976 embryo 08-10hr    7012 embryo 10-12hr    6215 embryo 12-14hr    9262 embryo 14-16hr    2403 embryo 16-18hr    3404 embryo 18-20hr    1758 embryo 20-22hr    1746 embryo 22-24hr    2907 larva L1    3337 larva L2    4839 larva L3 12hr old    3497 larva L3 puffstage 1-2    3021 larva L3 puffstage 3-6    3862 larva L3 puffstage 7-9    6767 white prepupae new    5690 white prepupae 12hr    5216 white prepupae 24hr    5504 pupae 2d postWPP    5525 pupae 3d postWPP    3944 pupae 4d postWPP    1507 adult male 01day    2668 adult male 05day    1999 adult male 30day    1630 adult female 01day    3146 adult female 05day    9641 adult female 30day    7039 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0000258    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0000258 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0000258 expression level Tissue   Expression Level Larval Central Nervous System    1329.575 Larval Midgut    844.2 Larval Hindgut    1201.7 Larval Malpighian Tubules    970.4 Larval Fat Body    1178.4 Larval Salivary Gland    1959.3 Larval Trachea    1542.45 Larval Carcass    1332.975 Adult Head    727.9 Adult Eye    1195.25 Adult Brain    1036.9 Adult Thoracic-Abdominal Ganglion    1106.6 Adult Crop    848.2 Adult Midgut    788.2 Adult Hindgut    771.6 Adult Malpighian Tubules    845.9 Adult Fat Body    955.5 Adult Salivary Gland    1330.4 Adult Heart    940.9 Adult VirginFemale Spermatheca    769.4 Adult InseminatedFemale Spermatheca    826 Adult Ovary    1605.6 Adult Testis    719.9 Adult Male Accessory Gland    1360 Adult Carcass    648.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1329.575) Larval Midgut  (844.2) Larval Hindgut  (1201.7) Larval Malpighian Tubules  (970.4) Larval Fat Body  (1178.4) Larval Salivary Gland  (1959.3) Larval Trachea  (1542.45) Larval Carcass  (1332.975) Adult Head  (727.9) Adult Eye  (1195.25) Adult Brain  (1036.9) Adult Thoracic-Abdominal Ganglion  (1106.6) Adult Crop  (848.2) Adult Midgut  (788.2) Adult Hindgut  (771.6) Adult Malpighian Tubules  (845.9) Adult Fat Body  (955.5) Adult Salivary Gland  (1330.4) Adult Heart  (940.9) Adult VirginFemale Spermatheca  (769.4) Adult InseminatedFemale Spermatheca  (826) Adult Ovary  (1605.6) Adult Testis  (719.9) Adult Male Accessory Gland  (1360) Adult Carcass  (648.6) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (1329.575) Larval Midgut    844.2 Larval Hindgut  (1201.7) Larval Malpighian Tubules    970.4 Larval Fat Body  (1178.4) Larval Salivary Gland  (1959.3) Larval Trachea  (1542.45) Larval Carcass  (1332.975) Adult Head    727.9 Adult Eye  (1195.25) Adult Brain  1036.9 Adult Thoracic-Abdominal Ganglion  (1106.6) Adult Crop    848.2 Adult Midgut    788.2 Adult Hindgut    771.6 Adult Malpighian Tubules    845.9 Adult Fat Body    955.5 Adult Salivary Gland  (1330.4) Adult Heart    940.9 Adult VirginFemale Spermatheca    769.4 Adult InseminatedFemale Spermatheca    826 Adult Ovary  (1605.6) Adult Testis    719.9 Adult Male Accessory Gland  (1360) Adult Carcass    648.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1329.575 Larval Midgut    844.2 Larval Hindgut    1201.7 Larval Malpighian Tubules    970.4 Larval Fat Body    1178.4 Larval Salivary Gland    1959.3 Larval Trachea    1542.45 Larval Carcass    1332.975 Adult Head    727.9 Adult Eye    1195.25 Adult Brain    1036.9 Adult Thoracic-Abdominal Ganglion    1106.6 Adult Crop    848.2 Adult Midgut    788.2 Adult Hindgut    771.6 Adult Malpighian Tubules    845.9 Adult Fat Body    955.5 Adult Salivary Gland    1330.4 Adult Heart    940.9 Adult VirginFemale Spermatheca    769.4 Adult InseminatedFemale Spermatheca    826 Adult Ovary    1605.6 Adult Testis    719.9 Adult Male Accessory Gland    1360 Adult Carcass    648.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0000258 expression level Tissue   Expression Level Larval Central Nervous System    1329.575 Larval Midgut    844.2 Larval Hindgut    1201.7 Larval Malpighian Tubules    970.4 Larval Fat Body    1178.4 Larval Salivary Gland    1959.3 Larval Trachea    1542.45 Larval Carcass    1332.975 Adult Head    727.9 Adult Eye    1195.25 Adult Brain    1036.9 Adult Thoracic-Abdominal Ganglion    1106.6 Adult Crop    848.2 Adult Midgut    788.2 Adult Hindgut    771.6 Adult Malpighian Tubules    845.9 Adult Fat Body    955.5 Adult Salivary Gland    1330.4 Adult Heart    940.9 Adult VirginFemale Spermatheca    769.4 Adult InseminatedFemale Spermatheca    826 Adult Ovary    1605.6 Adult Testis    719.9 Adult Male Accessory Gland    1360 Adult Carcass    648.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1329.575) Larval Midgut  (844.2) Larval Hindgut  (1201.7) Larval Malpighian Tubules  (970.4) Larval Fat Body  (1178.4) Larval Salivary Gland  (1959.3) Larval Trachea  (1542.45) Larval Carcass  (1332.975) Adult Head  727.9 Adult Eye  (1195.25) Adult Brain  (1036.9) Adult Thoracic-Abdominal Ganglion  (1106.6) Adult Crop  (848.2) Adult Midgut  788.2 Adult Hindgut  771.6 Adult Malpighian Tubules  (845.9) Adult Fat Body  (955.5) Adult Salivary Gland  (1330.4) Adult Heart  (940.9) Adult VirginFemale Spermatheca  769.4 Adult InseminatedFemale Spermatheca  (826) Adult Ovary  (1605.6) Adult Testis  719.9 Adult Male Accessory Gland  (1360) Adult Carcass  648.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1329.575 Larval Midgut    844.2 Larval Hindgut  1201.7 Larval Malpighian Tubules    970.4 Larval Fat Body  1178.4 Larval Salivary Gland  (1959.3) Larval Trachea  1542.45 Larval Carcass  1332.975 Adult Head    727.9 Adult Eye  1195.25 Adult Brain  1036.9 Adult Thoracic-Abdominal Ganglion  1106.6 Adult Crop    848.2 Adult Midgut    788.2 Adult Hindgut    771.6 Adult Malpighian Tubules    845.9 Adult Fat Body    955.5 Adult Salivary Gland  1330.4 Adult Heart    940.9 Adult VirginFemale Spermatheca    769.4 Adult InseminatedFemale Spermatheca    826 Adult Ovary  1605.6 Adult Testis    719.9 Adult Male Accessory Gland  1360 Adult Carcass    648.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1329.575 Larval Midgut    844.2 Larval Hindgut    1201.7 Larval Malpighian Tubules    970.4 Larval Fat Body    1178.4 Larval Salivary Gland    1959.3 Larval Trachea    1542.45 Larval Carcass    1332.975 Adult Head    727.9 Adult Eye    1195.25 Adult Brain    1036.9 Adult Thoracic-Abdominal Ganglion    1106.6 Adult Crop    848.2 Adult Midgut    788.2 Adult Hindgut    771.6 Adult Malpighian Tubules    845.9 Adult Fat Body    955.5 Adult Salivary Gland    1330.4 Adult Heart    940.9 Adult VirginFemale Spermatheca    769.4 Adult InseminatedFemale Spermatheca    826 Adult Ovary    1605.6 Adult Testis    719.9 Adult Male Accessory Gland    1360 Adult Carcass    648.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    727.9   NA  Eye    1195.25   NA  Brain    1036.9   1329.575  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1106.6   NA  Crop    848.2   844.2  Midgut    788.2   1201.7  Hindgut    771.6   970.4  Malpighian Tubules    845.9   1178.4  Fat Body    955.5   1959.3  Salivary Gland    1330.4   NA  Heart    940.9   1542.45  Trachea    NA   NA  VirginFemale Spermatheca    769.4   NA  InseminatedFemale Spermatheca    826   NA  Ovary    1605.6   NA  Testis    719.9   NA  Male Accessory Gland    1360   1332.975  Carcass    648.6 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_05
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000258 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG17520-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4hth-GAL4 CkIIαdsRNA.Scer\UAS lethal, with Scer\GAL4tsh-md621 CkIIαdsRNA.Scer\UAS lethal | heat sensitive, with Scer\GAL4h-H10 CkIIαdsRNA.Scer\UAS lethal | larval stage | recessive CkIIαTik lethal | recessive CkIIαTik CkIIαTikR Other Phenotypes Allele circadian rhythm defective, with Scer\GAL4P2.4.Pdf CkIIαScer\UAS.cLb locomotor rhythm defective CkIIαTik locomotor rhythm defective, with Scer\GAL4P2.4.Pdf CkIIαScer\UAS.cLa locomotor rhythm defective, with Scer\GAL4tim.PE CkIIαScer\UAS.cLa locomotor rhythm defective, with Scer\GAL4tim.Scer\UAS CkIIαScer\UAS.cLa locomotor rhythm defective | dominant CkIIαTik neuroanatomy defective | somatic clone | third instar larval stage CkIIαG703 CkIIαH3091 Phenotype manifest in Allele eye, with CkIIαdsRNA.Scer\UAS, Scer\GAL4ey.3.5.Exel CkIIαTik eye, with CkIIαTik, Scer\GAL4ey.3.5.Exel CkIIαdsRNA.Scer\UAS eye (with CkIIαdsRNA.Scer\UAS), with Scer\GAL4ey.3.5.Exel CkIIαTik eye (with CkIIαTik), with Scer\GAL4ey.3.5.Exel CkIIαdsRNA.Scer\UAS eye | heat sensitive, with Scer\GAL4ey.3.5.Exel CkIIαScer\UAS.Tik CkIIαdsRNA.Scer\UAS interommatidial bristle, with CkIIαdsRNA.Scer\UAS, Scer\GAL4ey.3.5.Exel CkIIαTik interommatidial bristle, with CkIIαTik, Scer\GAL4ey.3.5.Exel CkIIαdsRNA.Scer\UAS interommatidial bristle, with Scer\GAL4sca-537.4 CkIIαScer\UAS.Tik CkIIαdsRNA.Scer\UAS interommatidial bristle (with CkIIαdsRNA.Scer\UAS), with Scer\GAL4ey.3.5.Exel CkIIαTik interommatidial bristle (with CkIIαTik), with Scer\GAL4ey.3.5.Exel CkIIαdsRNA.Scer\UAS interommatidial bristle | heat sensitive, with Scer\GAL4ey.3.5.Exel CkIIαScer\UAS.Tik CkIIαdsRNA.Scer\UAS macrochaeta, with CkIIαdsRNA.Scer\UAS, Scer\GAL4sca-537.4 CkIIαTik macrochaeta, with CkIIαTik, Scer\GAL4sca-537.4 CkIIαdsRNA.Scer\UAS macrochaeta, with Scer\GAL4sca-537.4 CkIIαdsRNA.Scer\UAS macrochaeta (with CkIIαdsRNA.Scer\UAS), with Scer\GAL4sca-537.4 CkIIαTik macrochaeta (with CkIIαTik), with Scer\GAL4sca-537.4 CkIIαdsRNA.Scer\UAS mesothoracic bristle | supernumerary, with Scer\GAL4E(spl).Exel CkIIαdsRNA.Scer\UAS microchaeta, with Scer\GAL4sca-537.4 CkIIαdsRNA.Scer\UAS ommatidium, with CkIIαdsRNA.Scer\UAS, Scer\GAL4ey.3.5.Exel CkIIαTik ommatidium, with CkIIαTik, Scer\GAL4ey.3.5.Exel CkIIαdsRNA.Scer\UAS ommatidium (with CkIIαdsRNA.Scer\UAS), with Scer\GAL4ey.3.5.Exel CkIIαTik ommatidium (with CkIIαTik), with Scer\GAL4ey.3.5.Exel CkIIαdsRNA.Scer\UAS photoreceptor cell R8 | supernumerary, with Scer\GAL4ey.3.5.Exel CkIIαdsRNA.Scer\UAS scutellar bristle | supernumerary, with Scer\GAL4455.2 CkIIαdsRNA.Scer\UAS scutellar bristle | supernumerary, with Scer\GAL4E(spl).Exel CkIIαdsRNA.Scer\UAS scutellar bristle | supernumerary, with Scer\GAL4sca-537.4 CkIIαScer\UAS.Tik CkIIαdsRNA.Scer\UAS trichogen cell, with CkIIαdsRNA.Scer\UAS, Scer\GAL4sca-537.4 CkIIαTik trichogen cell, with CkIIαTik, Scer\GAL4sca-537.4 CkIIαdsRNA.Scer\UAS trichogen cell, with Scer\GAL4sca-537.4 CkIIαdsRNA.Scer\UAS trichogen cell (with CkIIαdsRNA.Scer\UAS), with Scer\GAL4sca-537.4 CkIIαTik trichogen cell (with CkIIαTik), with Scer\GAL4sca-537.4 CkIIαdsRNA.Scer\UAS
Classical Alleles ( 5 )
For All Classical Alleles Show
Allele of CkIIα Class Mutagen Stocks Known lesion CkIIαMB00477 1 -- CkIIαTik ethyl methanesulfonate 1 Yes CkIIαTikR 1 Yes CkIIαG703 ethyl methanesulfonate 0 Yes CkIIαH3091 ethyl methanesulfonate 0 Yes
Alleles Carried on Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CkIIα Class Mutagen Stocks Known lesion CkIIαGL00003 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CkIIαJF01436 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CkIIαScer\UAS.cLa in vitro construct - regulatory fusion 1 Yes CkIIαScer\UAS.Tik in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement 1 Yes CkIIαdsRNA.cYa in vitro construct - RNAi 0 Yes CkIIαdsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes CkIIαGD9403 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes CkIIαGMR.PB in vitro construct - regulatory fusion 0 Yes CkIIαKM.Scer\UAS.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes CkIIαScer\UAS.cLb in vitro construct - regulatory fusion 0 Yes CkIIαScer\UAS.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data insertion of enhancer trap binary system Mi{ET1}CkIIαMB00477 No
Gene Ontology: Function, Process & Cellular Component ( 21 unique terms )
Terms Based on Experimental Evidence ( 19 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with CkIIα-i1 (Kalive et al., 2001) protein serine/threonine kinase activity inferred from direct assay (Siomi et al., 2002, Glover et al., 1983, Dahmus et al., 1984)
Biological Process
CV term Evidence References
axon guidance inferred from mutant phenotype (Berger et al., 2008) chaeta development inferred from mutant phenotype (Bose et al., 2006) circadian rhythm inferred from mutant phenotype (Lin et al., 2005) compound eye development inferred from mutant phenotype (Karandikar et al., 2004, Bose et al., 2006) lateral inhibition inferred from mutant phenotype (Bose et al., 2006) locomotor rhythm inferred from mutant phenotype (Lin et al., 2002, Akten et al., 2009) mitosis inferred from mutant phenotype (Ducat et al., 2008) negative regulation of protein ubiquitination inferred from mutant phenotype (Jia et al., 2010) positive regulation of hh target transcription factor activity inferred from mutant phenotype (Jia et al., 2010) positive regulation of smoothened signaling pathway inferred from mutant phenotype (Jia et al., 2010) protein phosphorylation inferred from direct assay (Glover et al., 1983) regulation of protein stability inferred from mutant phenotype (Jia et al., 2010) Wnt receptor signaling pathway inferred from direct assay (Willert et al., 1997)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Siomi et al., 2002) cytosol inferred from direct assay (Glover et al., 1983) nucleus inferred from direct assay (Glover et al., 1983) protein kinase CK2 complex inferred from direct assay (Glover et al., 1983, Dahmus et al., 1984)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441 (FlyBase Curators et al., 2004-) protein kinase activity non-traceable author statement (Morrison et al., 2000) protein serine/threonine kinase activity inferred from sequence or structural similarity (Saxena et al., 1987) inferred from sequence or structural similarity with EMBL:U51866 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1988.8.1)
Biological Process
CV term Evidence References
protein phosphorylation inferred from sequence or structural similarity (Saxena et al., 1987) non-traceable author statement (Morrison et al., 2000, Swiss-Prot Project Members, 1988.8.1)
Cellular Component
CV term Evidence References
protein kinase CK2 complex inferred from sequence or structural similarity (Saxena et al., 1987) non-traceable author statement (Swiss-Prot Project Members, 1988.8.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CkIIα allele Gene References unspecified E(spl) (Bose et al., 2006) per (Lin et al., 2005)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 71396 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0000258 DroID - A comprehensive database of gene and protein interactions. • FBgn0000258 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 48448
Orthologs
Genome-wide drosophilid orthologs	Dana\GF11011 Dere\GG13159 Dgri\GH22328 Dmoj\GI23626 Dper\GL21875 Dpse\GA22219 Dsec\GM22493 Dsim\CkIIα Dvir\GJ11189 Dwil\GK12170 Dyak\CkIIα
Curated drosophilid orthologs	Dmoj\CkIIα (Sztal et al., 2007) Dmon\CkIIα (Kankare et al., 2010) Dsim\CkIIα   Dyak\CkIIα
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL012094-PA Aedes aegypti (mosquito) AGAP005569-PA Anopheles gambiae (mosquito) XP_623397.1 Apis mellifera (honey bee) XP_001942962.1 Acyrthosiphon pisum (pea aphid) At2g23070.1 Arabidopsis thaliana (wall cress) 20828 Batrachochytrium dendrobatidis (chytrid fungus) 113587 Branchiostoma floridae (lancelet) 262877 Branchiostoma floridae (lancelet) BGIBMGA003474-PA Bombyx mori (silk worm) ENSBTAP00000016371 Bos taurus (bovine) CAL0000912 Candida albicans (a form of yeast) CN07016 Caenorhabditis brenneri (small nematode) CBP03097 Caenorhabditis briggsae (nematode) CE17321 Caenorhabditis elegans (worm) ENSCAFP00000010339 Canis familiaris (dog) CAGL0I05192g Candida glabrata (haploid yeast) Chro.60083 Cryptosporidium hominis (obligate parasite) ENSCINP00000019380 Ciona intestinalis (sea squirt) JA14520 Caenorhabditis japonica (nematode) CMP128C Cyanidioschyzon merolae (red alga) cn06193 Cryptococcus neoformans (yeast-like fungus) cgd6_620 Cryptosporidium parvum (protozoan parasite) CPIJ011540 Culex pipens (mosquito) ENSCPOP00000005167 Cavia porcellus (guinea pig) 194436 Chlamidomonas reinhardtii (green alga) RP13506 Caenorhabditis remanei (nematode) ENSCSAVP00000015808 Ciona savignyi (sedentary tunicate) 162678 Capitella spl (polychaete worm) DDB0185025 Dictyostelium discoideum (amoeba) DEHA2A13662g Debaryomyces Hansenii (yeast) 223674 Daphnia pulex (daphnia) ENSDARP00000006001 Danio rerio (zebra fish) ENSECAP00000005940 Equus caballus (horse) XP_654466 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000015084 Gymnopilus aculeatus (mushroom) ENSGALP00000009985 Gallus gallus (chicken) GL50803_27520 Giardia lamblia (flagellated protozoan) 90804 Hypsipyla robusta (cedar shoot borer) ENSP00000371400 Homo sapiens (human) ISCW007259-PA Ixodes scapularis (deer tick) KLLA0E04247g Kluyveromyces lactis (yeast) 184685 Lottia gigantea (owl limpet) LmjF35.1730 Leishmania major (virus) 35210 Monocosta brevicollis (choanoflagellate) ENSMODP00000024355 Monodelphis domestica (gray opossum) ENSMMUP00000037657 Macaca mulatta (rhesus monkey) ENSMUSP00000096829 Mus musculus (house mouse) 166091 Nematostella vectensis (starlet anemone) ENSOANP00000019186 Ornithorynchus anatinus (platypus) ENSORLP00000008490 Oryzias latipes (japanese killifish) LOC_Os07g02350.1 Oryza sativa (asian rice) 8917 Ostreococcus tauri (green alga) PF11_0096 Plasmodium falciparum (protozoal parasite) PHUM002603-PA Pediculus humanus (human body louse) PP10068 Pristionchus pacificus (nematode) 145621 Physcomitrella patens (moss) ENSPPYP00000012136 Pongo pygmaeus (orangutan) 72121 Phytophthora ramorum (sudden oak death pathogen) 109488 Phytophthora sojae (root rot pathogen) 414425 Populus trichocarpa (black cottonwood) 716324 Populus trichocarpa (black cottonwood) ENSPTRP00000022508 Pan troglodytes (chimpanzee) PVX_091095 Plasmodium vivax (protozoal parasite) PVX_252300 Plasmodium vivax (protozoal parasite) ENSRNOP00000007558 Rattus norvegicus (norway rat) 4782420 Sorghum bicolor (sorghum) YIL035C Saccharomyces cerevisiae (budding yeast) Smp_099030 Schistosoma mansoni (trematode parasite) SPAC23C11.11 Schizosaccharomyces pombe (fission yeast) XP_001176839.1 Strongylocentrotus purpuratus (purple sea urchin) 50201 Trichoplax adhaerens (metazoa) TA10630 Theileria annulata (apicomplexan parasite) XP_972046.2 Tribolium castaneum (red flour beetle) Tc00.1047053510761.60 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000006402 Tetraodon nigroviridis (green spotted pufferfish) TP04_0659 Theileria parva (parasitic protozoan) 39539 Thalassiosira pseudonana (marine diatom) ENSTRUP00000007254 Takifugu rubripes (pufferfish Takifugu) 3820.m00272 Tetrahymena thermophila (ciliate protozoa) 82397.m00111 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000030055 Xenopus tropicalis (frog) YALI0D16863g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB15544 LH12677 ISCW007259 FBgn0046892 FBgn0214181 FBgn0198450 FBgn0046914 FBgn0177363 JGI_V11_223674 FBgn0243626 FBgn0159468 FBgn0146352 FBgn0129786 FBgn0105432 FBgn0088051 CPIJ011540 BGIBMGA003474 ACYPI002006 GB11423 AGAP005569 AAEL012094 PHUM404140 GLEAN_10853
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	24625 y1 w*; P{UAS-CkIIα.L}35 24624 y1 w*; P{UAS-CkIIα.Tik}T1 31645 y1 v1; P{TRiP.JF01436}attP2 35136 y1 sc* v1; P{TRiP.GL00003}attP2 24511 CkIIαTikR/TM3, P{GAL4-Kr.C}DC2, P{UAS-GFP.S65T}DC10, Sb1 22812 y1 w67c23; Mi{ET1}CkIIαMB00477/TM3, Sb1 Ser1 24512 y1 w*; CkIIαTik/TM3, P{GAL4-Kr.C}DC2, P{UAS-GFP.S65T}DC10, Sb1
Genomic Clones ( 2 )
	BACR17M18 BACR29A07 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 137 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD27706
Other clones	DPiM_LD27706
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT08183 AT15417 GM04859 GM06353 LD01014 LD11113 LP02330 LP05067 RE04052 RE04120 RE11341 RE21670 RE29742 RE33349 RE46590 RH18365 RH19214 SD15912
Other clones	37810 67269 118616 121115 122952 124560 124617 147170 147978 148050 191752 CK01.44B.C7 Dmel_dig_015071_0708_1605 Dmel_dig_019397_0699_3033 Dmel_neb_011931_0675_2856 EC26020 EC055454 EK150156 EK172714 EK197306 EK240707 EK246046 EK246945 EK250410 EK251421 EK251503 EK253747 EK294247 EN03502 ESG012c.E21_D01 FGC026B01 FGM231B11 RP004007701 RP004015397 RP004030057 RP004031692 RP004052159 RP004059322 RP004061893 RP004075987 RP004080508 RP004082763 RP004089830 RP004094148 RP004106880 RP004117628 RP004123578 RP004123593 RP004124869 RP004127688 RP004132554 RP004135476 RP004152788 RP004158491 RP004174343 RP004177174 RP004185159 RP004188637 RP004198326 RP004201033 RP004206953 RP004214405 RP004217058 RP004222118 RP004222526 RP004225794 RP004230413 RP004231404 RP004233292 RP004235020 RP004245296 RP004254808 RP004261194 RP004262277 RP004263137 RP004264197 RP004268718 RP004288725 RP004327272 RP004333529 RP004342885 RP004347780 RP004360985 RP004368738 RP004372187 RP004376111 RP004377263 RP004387004 RP004388442 RP004391544 RP004405185 RP004406149 RP004409376 RP004419379 RP004427396 RP004427715 RP004430139 RP004435015 RP004436545 RP004447331 RP004465226 RP004481105 RP004487123 RP004487316 RP004488420 RP004492491 RP004496063 RP004497643 RP004504301 RP004537854 RP004538600 RP004544182 RP004554649 UUGC0157 UUGC0526 UUGC0815 UUGC1040
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0000258 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 48448
Antibody Information
	polyclonal (Dahmus et al., 1984)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: CkIIα anon-WO02059370.53 (FlyBase, 1996-) Source for merge of: CkIIα Tik (Lin et al., 2002)
Additional comments	Complementation tests and recombinational mapping suggest the CkIIα mutant maps to a novel circadian rhythm gene. (Allada et al., 2002) Source for merge of CkIIα anon-WO02059370.53 was sequence comparison (date:051113). (FlyBase, 1996-) Drosophila Casein kinase II α and β subunits can rescue the lethality of S.cerevisiae deleted for both genes. (Bidwai et al., 1992)
Other Comments
	When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G2/M phase cells, a whole range of mitotic abnormalities, centrosome abnormalities and lagging chromatids are seen. (Bettencourt-Dias et al., 2004) CkIIα may regulate eye morphogenesis via phosphorylation of E(spl). (Karandikar et al., 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Identification: screen for circadian locomotor behavior mutants. (Allada et al., 2002) A dominant mutant, which has a long period phenotype as a heterozygote. Homozygotes die during the third larval instar stage. The recessive lethal is not separable from the circadian phenotype, suggesting a vital role for the CkIIα gene. (Allada et al., 2002) In vivo activity of Antp is modified by CkIIα-mediated phosphorylation. Phosphorylation of Antp by CkIIα is important for preventing inappropriate activities of this homeotic protein during embryogenesis. (Jaffe et al., 1997) Casein kinase II specifically phosphorylates a set of serine residues within the cact PEST domain. (Liu et al., 1997) Casein kinase II phosphorylates Ser468 (a residue in the PEST domain) of cact in vitro. (Packman et al., 1997) Casein kinase II (encoded by CkIIα and CkIIβ) phosphorylates dsh in vitro. This phosphorylation is regulated by fz2, implicating a role for Casein kinase II in wg signalling. (Willert et al., 1997) Recombinant CkII in vitro binds to spermine and this interaction is concomitant with a striking effect on the structural polymeric organisation on the kinase which in the presence of the polyamine exhibits a ring-like structure. (Leroy et al., 1994) Casein kinase II reconstituted from heterologous subunits displays properties similar to those of the homologous protein. (Lin et al., 1994) CkIIβ is primarily an inhibitor of the catalytic activity of CkIIα in the absence of salt. (Tiganis et al., 1993) Casein kinase II-mediated phosphorylation of Top2 stimulates its activity by enhancing the ability of the enzyme to hydrolyse its high energy ATP cofactor. (Corbett et al., 1992) S.cerevisiae Scer\cka1 Scer\cka2 double mutants are functionally complemented by CkIIα. (Birnbaum and Glover, 1991) Isolation and sequencing of cDNA clones encoding both subunits of casein kinase II. (Saxena et al., 1987)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080179 NM_168985 NM_168986 RefSeq (Proteins) • NP_524918 NP_730774 NP_730775 DDBJ / EMBL / GenBank • AA948982 AAA28429 AAF45439 AAL39698 AAN11415 AAN11416 AW942772 AX664479 AY069553 M16534 Entrez Gene - A searchable database of RefSeq genes. • 48448 UniProtKB/Swiss-Prot • P08181
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine-protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) Serine/threonine-protein kinase-like domain (IPR017442)
Linkouts
	BioGRID - A database of protein and genetic interactions • 71396 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0000258 DroID - A comprehensive database of gene and protein interactions. • FBgn0000258 DRSC - Results from RNAi screens. • FBgn0000258 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000258 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG17520-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0000258 FlyReactome - A curated knowledgebase of Drosophila melanogaster pathways • 93710 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 48448 InParanoid A subset of ortholog calls from InParanoid. • AAEL012094-PA Aedes aegypti (mosquito) AGAP005569-PA Anopheles gambiae (mosquito) XP_623397.1 Apis mellifera (honey bee) XP_001942962.1 Acyrthosiphon pisum (pea aphid) At2g23070.1 Arabidopsis thaliana (wall cress) 20828 Batrachochytrium dendrobatidis (chytrid fungus) 113587 Branchiostoma floridae (lancelet) 262877 Branchiostoma floridae (lancelet) BGIBMGA003474-PA Bombyx mori (silk worm) ENSBTAP00000016371 Bos taurus (bovine) CAL0000912 Candida albicans (a form of yeast) CN07016 Caenorhabditis brenneri (small nematode) CBP03097 Caenorhabditis briggsae (nematode) CE17321 Caenorhabditis elegans (worm) ENSCAFP00000010339 Canis familiaris (dog) CAGL0I05192g Candida glabrata (haploid yeast) Chro.60083 Cryptosporidium hominis (obligate parasite) ENSCINP00000019380 Ciona intestinalis (sea squirt) JA14520 Caenorhabditis japonica (nematode) CMP128C Cyanidioschyzon merolae (red alga) cn06193 Cryptococcus neoformans (yeast-like fungus) cgd6_620 Cryptosporidium parvum (protozoan parasite) CPIJ011540 Culex pipens (mosquito) ENSCPOP00000005167 Cavia porcellus (guinea pig) 194436 Chlamidomonas reinhardtii (green alga) RP13506 Caenorhabditis remanei (nematode) ENSCSAVP00000015808 Ciona savignyi (sedentary tunicate) 162678 Capitella spl (polychaete worm) DDB0185025 Dictyostelium discoideum (amoeba) DEHA2A13662g Debaryomyces Hansenii (yeast) 223674 Daphnia pulex (daphnia) ENSDARP00000006001 Danio rerio (zebra fish) ENSECAP00000005940 Equus caballus (horse) XP_654466 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000015084 Gymnopilus aculeatus (mushroom) ENSGALP00000009985 Gallus gallus (chicken) GL50803_27520 Giardia lamblia (flagellated protozoan) 90804 Hypsipyla robusta (cedar shoot borer) ENSP00000371400 Homo sapiens (human) ISCW007259-PA Ixodes scapularis (deer tick) KLLA0E04247g Kluyveromyces lactis (yeast) 184685 Lottia gigantea (owl limpet) LmjF35.1730 Leishmania major (virus) 35210 Monocosta brevicollis (choanoflagellate) ENSMODP00000024355 Monodelphis domestica (gray opossum) ENSMMUP00000037657 Macaca mulatta (rhesus monkey) ENSMUSP00000096829 Mus musculus (house mouse) 166091 Nematostella vectensis (starlet anemone) ENSOANP00000019186 Ornithorynchus anatinus (platypus) ENSORLP00000008490 Oryzias latipes (japanese killifish) LOC_Os07g02350.1 Oryza sativa (asian rice) 8917 Ostreococcus tauri (green alga) PF11_0096 Plasmodium falciparum (protozoal parasite) PHUM002603-PA Pediculus humanus (human body louse) PP10068 Pristionchus pacificus (nematode) 145621 Physcomitrella patens (moss) ENSPPYP00000012136 Pongo pygmaeus (orangutan) 72121 Phytophthora ramorum (sudden oak death pathogen) 109488 Phytophthora sojae (root rot pathogen) 414425 Populus trichocarpa (black cottonwood) 716324 Populus trichocarpa (black cottonwood) ENSPTRP00000022508 Pan troglodytes (chimpanzee) PVX_091095 Plasmodium vivax (protozoal parasite) PVX_252300 Plasmodium vivax (protozoal parasite) ENSRNOP00000007558 Rattus norvegicus (norway rat) 4782420 Sorghum bicolor (sorghum) YIL035C Saccharomyces cerevisiae (budding yeast) Smp_099030 Schistosoma mansoni (trematode parasite) SPAC23C11.11 Schizosaccharomyces pombe (fission yeast) XP_001176839.1 Strongylocentrotus purpuratus (purple sea urchin) 50201 Trichoplax adhaerens (metazoa) TA10630 Theileria annulata (apicomplexan parasite) XP_972046.2 Tribolium castaneum (red flour beetle) Tc00.1047053510761.60 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000006402 Tetraodon nigroviridis (green spotted pufferfish) TP04_0659 Theileria parva (parasitic protozoan) 39539 Thalassiosira pseudonana (marine diatom) ENSTRUP00000007254 Takifugu rubripes (pufferfish Takifugu) 3820.m00272 Tetrahymena thermophila (ciliate protozoa) 82397.m00111 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000030055 Xenopus tropicalis (frog) YALI0D16863g Yarrowia lipolytica (nonconventional yeast) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /dbzhnsky/cask2-1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 48448 modMine - Data generated by the modENCODE project. • FBgn0000258 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB15544 LH12677 ISCW007259 FBgn0046892 FBgn0214181 FBgn0198450 FBgn0046914 FBgn0177363 JGI_V11_223674 FBgn0243626 FBgn0159468 FBgn0146352 FBgn0129786 FBgn0105432 FBgn0088051 CPIJ011540 BGIBMGA003474 ACYPI002006 GB11423 AGAP005569 AAEL012094 PHUM404140 GLEAN_10853
Synonyms & Secondary IDs ( 43 )
Reported As
Symbol Synonym	anon-WO02059370.53   Cask-II-a   CG17520 (Morrison et al., 2000, Ducat et al., 2008, Berger et al., 2008, Ni et al., 2008, Ni et al., 2011.7.19, Cammarato et al., 2011) CK2 (Hardin, 2005, Tie et al., 2005, Karandikar et al., 2004, Wharton, 2003, Li et al., 1999, Nusse et al., 1997, Willert et al., 1997, Valero et al., 1997, Cadigan and Nusse, 1997, Leroy et al., 1997, Filhol et al., 1996, Kahali et al., 2006, Bose et al., 2006, Remus et al., 2005, Lin et al., 2005, Bose et al., 2006, Bose et al., 2008, Yu and Hardin, 2006) CK-2 (Goueli and Ahmed, 1991) CK2a (Allada et al., 2003) CK2alpha (Jauch and Raabe, 2003) CK2 alpha (Li et al., 1999) CK2α (Kim and Edery, 2006, Akten et al., 2003, Lin et al., 2002, Kalmykova et al., 2002, Smith et al., 2006, Jauch et al., 2006, Kahali et al., 2008, Smith et al., 2008, Kahali et al., 2009, Stark et al., 2011, Jia et al., 2010) Ck2α (Meissner et al., 2008) CK2-α (Lear et al., 2005) CKII (Schoning and Staiger, 2005, Nawathean and Rosbash, 2004, Horiuchi et al., 2004, Bandhakavi et al., 2003, Vervoort, 2002, Eissenberg and Elgin, 2000, Waggener and DiMario, 1999, Zhao and Eissenberg, 1999, Brown and Moon, 1998, Liu et al., 1997, Wilson et al., 1997, Tsubota et al., 1997, Liu et al., 1997, Packman et al., 1997, Corbett et al., 1993, Gelsthorpe et al., 2006, Li et al., 2002) CK-II (Dworak and Sink, 2002) CK II (Horiuchi et al., 2000) CkII (Anonymous, 1998, Jaffe et al., 1997) CKIIa (Morrison et al., 2000) CKIIalfa (Dimitri et al., 2005) CKIIalpha (Greil et al., 2003, Gonzalez et al., 2002) CkIIalpha (Giot et al., 2003, Wasbrough et al., 2010) CKII alpha (Zhang et al., 2010) CKIIα (Karandikar et al., 2003, Lee et al., 1999, Nawathean et al., 2007, Tadros et al., 2007, Hsu et al., 2008, Andersen et al., 2010, Guenin et al., 2010) CKII-α (Yanagawa et al., 2002) CK-II α (Bourbon et al., 1995) CkIIα (Akten et al., 2009, Fontenele et al., 2009, Yang et al., 2008) ckIIα (Berger et al., 2008) dCK2α (Bouazoune and Brehm, 2005, Sawatsubashi et al., 2010) dCKII (Jin and Warren, 2003) dCKII alpha (Siomi et al., 2002) DmCK2alpha (Mentzel and Raabe, 2005) DmCK2α (Kalive et al., 2001, Mentzel et al., 2009) DmCKIIα (Zhao et al., 2002, Trott et al., 2001) Tik (Allada et al., 2002, Kunttas et al., 2007, Kahali et al., 2008, Kunttas-Tatli et al., 2009, Meissner et al., 2008, Lear et al., 2005) αCK2 (Bonet et al., 2005)
Name Synonym	casein kinase 2 (Van Gelder et al., 2003, Remus et al., 2005, Yu and Hardin, 2006, Bonet et al., 2005) Casein kinase II (Liu et al., 1997, Corbett et al., 1992) casein kinase II (Zhang et al., 2010) Casein kinase II alpha subunit (Cammarato et al., 2011) Casein kinase II α subunit   Casein kinase II-α subunit (Ducat et al., 2008) NAP1-kinase (Li et al., 1999) protein kinase CK2 (Kunttas et al., 2007) Timekeeper (Allada et al., 2002, Kunttas-Tatli et al., 2009, Hall, 2003, Lear et al., 2005) α subunit of Drosophila casein kinase II (Birnbaum and Glover, 1991)
Secondary FlyBase IDs
	FBgn0062248 FBgn0066006
References ( 137 )
Generate a list of	All references relating to this gene ( 137 )
List References by type	Research paper  ( 82 ) Supplementary material  ( 7 ) Review  ( 17 ) Personal communication to FlyBase  ( 4 ) Abstract  ( 17 ) FlyBase analysis  ( 2 ) Conference report  ( 1 ) Patent  ( 1 ) Protein sequence record  ( 1 ) Computer file  ( 2 ) Automatic genome annotation  ( 1 ) Book  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 8 )
	Stark et al., 2011, Cell. Signal. 23(5): 876--882 Protein kinase CK2 links polyamine metabolism to MAPK signalling in Drosophila. [FBrf0213122] Wong et al., 2011, PLoS ONE 6(9): e24355 The Functioning of the Drosophila CPEB Protein Orb Is Regulated by Phosphorylation and Requires Casein Kinase 2 Activity. [FBrf0216252] Andersen et al., 2010, Mol. Cell. Biol. 30(19): 4744--4755 Drosophila MCRS2 Associates with RNA Polymerase II Complexes To Regulate Transcription. [FBrf0211794] Guenin et al., 2010, BMC Genomics 11: 47 Expression profiling of prospero in the Drosophila larval chemosensory organ: Between growth and outgrowth. [FBrf0210012] Jia et al., 2010, J. Biol. Chem. 285(48): 37218--37226 Casein kinase 2 promotes Hedgehog signaling by regulating both smoothened and Cubitus interruptus. [FBrf0214177] Kankare et al., 2010, BMC Ecol. 10(1): 3 Changes in gene expression linked with adult reproductive diapause in a northern malt fly species: a candidate gene microarray study. [FBrf0210028] Sawatsubashi et al., 2010, Genes Dev. 24(2): 159--170 A histone chaperone, DEK, transcriptionally coactivates a nuclear receptor. [FBrf0209754] Zhang et al., 2010, Genetics 184(4): 1165--1179 A genomewide RNA interference screen for modifiers of aggregates formation by mutant Huntingtin in Drosophila. [FBrf0210722] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...