Gene model reviewed during 5.45
There is only one protein coding transcript and one polypeptide associated with this gene
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cat using the Feature Mapper tool.
Cat enzyme activity is detected throughout development with peaks in late third instar just before puparium formation (at 96-100hr after egg deposition) and during metamorphosis at approx. 190 hours after egg deposition.
The level of Catenzyme activity in the Catn2 mutant was shown to be less than 10% ofwild type levels throughout development. There is a correspondingly lowlevel of Cat-specific cross-reacting material on Western blots of theCatn2/Df(3L)Cat mutant.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Cat in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. Shows significant change of expression pattern in circadian mutant background; decreased expression in per01, tim01 and ClkJrk background.
Overexpression of Cat significantly increases resistance to hydrogen peroxide but has neutral or slightly deleterious effects on adult life span.
mRNA levels increase at adult day 5 in strain showing extended longevity phenotype (ELP).
Transgenic flies carrying three copies of Sod+ and three copies of Cat+ exhibited as much as one third extension of life span, a longer mortality rate doubling time, a lower amount of protein oxidative damage and a delayed loss in physical performance. Results support the free radical hypothesis of aging.
Viability of acatalasemic flies can be restored by transformation with the wild type catalase gene. Though lack of catalase activity causes decreased viability and life span, increasing catalase activity above wild type levels had no effect on normal life span.
Identification: Identified in 2D gels of CMW W2 wing imaginal disc cell proteins.
Overexpression of Cat demonstrates enhanced levels of Cat does not prolong the life span, nor does it provide improved protection against oxidative stress induced by hyperoxia or paraquat treatment. It can provide enhanced resistance to hydrogen peroxide.
D.melanogaster sensitivity to the deleterious effects of hydrogen peroxide increases when catalase activity is relatively low.
The structural gene for catalase, a tetrameric enzyme. Two peaks of activity, the smaller in late third instar larvae just prior to puparium formation and the larger during metamorphosis; coincident with the two major peaks of ecdysone titer. High specific activity in larval Malpighian tubules, gut and fat body; higher in adult abdomen than in thorax or head. Amorphic and hypomorphic mutants are hemizygous viable on normal medium; however those with the lowest levels of catalase activity exhibit severely reduced viability (i.e., less than 2% normal levels). All mutants show increased sensitivity to the presence of hydrogen peroxide in the medium. No electromorphs detected among 50 inbred laboratory strains.