PKA, DC0, DCO, PKAc, protein kinase A
catalytic subunit of PKA - involved in Hedgehog signaling pathway and functions in learning in the adult - contributes to axis specification, rhythmic behavior and synaptic transmission
Please see the JBrowse view of Dmel\Pka-C1 for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
A non-AUG start codon may be used for translation of one or more transcripts of this gene; based on the presence of conserved protein signatures within the 5' UTR without an in-frame AUG (FBrf0243886).
5.1, 4.1, 3.4 (northern blot)
5.1, 4.1, 3.4, 2.4 (northern blot)
352 (aa)
353 (aa)
40 (kD observed)
Composed of two regulatory chains and two catalytic chains.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pka-C1 using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: maternally deposited
Pka-C1 transcripts are observed during oogenesis. Transcripts are first observed in the germarium, are present at slightly higher levels in the oocyte relative to nurse cells in stages 5-6 and are sometimes transiently anteriorly localized at stage 8.
Pka-C1 transcripts are detected predominantly in the perikarya of the mushroom bodies. Weak signal is observed over the cortex of the central brain, optic lobes, and thoracic ganglia.
A strong Pka-C1 transcript of 5.1kb is observed in adults. Weaker transcripts of 4.1kb and 3.4kb are also observed.
Pka-C1 protein is detected in all parts of all Kenyon cells in freshly eclosed adults.
Expression of Pka-C1 is within the presumptive mushroom body neuroblasts.
Pka-C1 protein is strongly expressed in the mushroom body alpha, beta and gamma lobes, spur and calyx. It is moderately expressed in the mushroom body alpha' and beta' lobes.
Pka-C1 protein is associated with germ cell membranes throughout oogenesis.
Pka-C1 protein is preferentially expressed in mushroom body neurons in adult heads.
Pka-C1 protein activity is detected at all developmental stages.
JBrowse - Visual display of RNA-Seq signals
View Dmel\Pka-C1 in JBrowse2-36
2-31.3
Pka-C1Cos-9, Pka-C1Cos-2, Pka-C1Cos-3 and Pka-C1Cos-8 map near 30C by recombination mapping.
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
Pka-C1 is necessary for the death and resorption of wing blade cells after eclosion.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
2 alleles of Pka-C1 been recovered in a screen for mutations with mutant phenotypes in clones in the wing.
Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.
Identification: Enhancer trap screen designed to discover genes involved in the cellular aspects of defense mechanisms, as well as in melanotic tumor formation processes linked to blood cell disregulation.
Each primordia of the genital disc (female genital, male genital and anal primordia) is divided into anterior and posterior compartments. Genes known to be expressed in compartment-specific manner in discs are expressed in analogous patterns in each primordia.
Pka-C1 plays a critical role in the flow of temporal information from circadian pacemaker cells to specific downstream targets controlling overt behaviours.
Pka-C1 is involved in medium-term memory formation.
The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation. Pka-C1 is required for germ cell viability or early oogenesis.
Pka-C1 is essential during limb development to prevent inappropriate dpp and wg expression. A constitutively active form of Mmus\Pkaca, can prevent inappropriate dpp and wg expression but does not interfere with their normal induction by hh. The basal activity of Pka-C1 imposes a block on the transcription of dpp and wg and hh exerts its organizing influence by alleviating the block.
Pka-C1 is a component of the signal transduction system through which cell interactions control pattern formation in the developing imaginal discs.
hh pathway mutants induce ectopic morphogenetic furrows. Results show that ommatidial clusters are self-organising units whose polarity in one axis is determined by the direction of furrow progression and which can independently define the position of an equator without reference to the global coordinates of the eye disc.
Mutations in ptc show strong-non-autonomous effects in clones induced in the developing eye. Both ptc and Pka-C1 are required for the correct regulation of morphogenetic furrow progression, apparently via repression of dpp. Loss of function of either ptc or Pka-C1 in cells anterior to the furrow results in an ectopic furrow characterised by non-autonomous propagation of dpp expression outside the mutant tissue and ectopic photoreceptor differentiation. Both ptc and Pka-C1 act downstream of hh.
Pka-C1 is required for the correct spatial regulation of dpp expression during eye development. Loss of Pka-C1 function is sufficient to produce an ectopic morphogenetic wave marked by premature ectopic photoreceptor differentiation and non-autonomous propagation of dpp expression. Results indicate that Pka-C1 lies in a signalling pathway that controls the orderly temporal progression of differentiation across the eye imaginal disc.
Pka-C1 is required for normal adult rhythms.
Pka-C1 plays a crucial role in the cAMP cascade in mushroom bodies to mediate learning and memory processes.
Purification and characterisation of the catalytic subunit of cAMP- dependent protein kinase.
Pole cell transplantation, genetic mosaics and isolation of conditional alleles have been used to determine the function of Pka-C1 during embryogenesis and imaginal disc development.
Pka-C1 encodes a highly conserved catalytic subunit of the cAMP-dependent protein kinase.
The dominant phenotype seen in flies heterozygous for the neomorphic "Cos" mutants is the result of a reduced level of function at the cos locus. Haploidy for cos enhances, whereas triploidy for cos suppresses the phenotype of "Cos" heterozygotes. Mutant embryos show phenotypes similar to those caused by mutants at the cos locus.
Encodes one of the isoforms of the catalytic subunit form of Pka. Drosophila cAMP-dependent protein kinase has been purified from the bodies of adult flies; the enzyme is made up of catalytic and regulatory subunits (FBrf0041205). The catalytic subunit has a molecular weight of 40,000 and the regulatory subunit a molecular weight of 52,000 or 58,000 (based on electrophoretic mobilities in sodium dodecyl sulfate-polyacrylamide gels).
