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General Information
Symbol
Dmel\Pka-C1
Species
D. melanogaster
Name
Protein kinase, cAMP-dependent, catalytic subunit 1
Annotation Symbol
CG4379
Feature Type
FlyBase ID
FBgn0000273
Gene Model Status
Stock Availability
Enzyme Name (EC)
cAMP-dependent protein kinase (2.7.11.11)
Gene Snapshot
Protein kinase, cAMP-dependent, catalytic subunit 1 (Pka-C1) encodes a serine/threonine kinase that contributes to axis specification, rhythmic behavior and synaptic transmission. [Date last reviewed: 2019-03-14]
Also Known As
PKA, DC0, DCO, PKAc, protein kinase A
Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:9,682,315..9,699,297 [-]
Recombination map
2-36
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. (P12370)
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
ATP + a protein = ADP + a phosphoprotein (2.7.11.11)
Summaries
Gene Group (FlyBase)
PROTEIN KINASE A FAMILY -
The Protein Kinase A (PKA) or cAMP-dependent protein kinases are serine/threonine kinases activated by cAMP. The PKA catalytic subunit is maintained in an inactive state in a heterotetrameric complex consisting of two catalytic (C) subunits bound to two regulatory (R) subunits. Binding of cAMP to the R subunits, releases the C subunits from inhibition. (Adapted from PMID:20027184).
Pathway (FlyBase)
Negative Regulators of Hedgehog Signaling Pathway -
Negative regulators of hedgehog signaling down-regulation the pathway, resulting in the repression of transcription of hh-responsive genes.
Positive Regulators of Hedgehog Signaling Pathway -
Positive regulators of hedgehog signaling down-regulation the pathway, enhancing the activation of transcription of hh-responsive genes.
Protein Function (UniProtKB)
Serine/threonine-protein kinase involved in memory formation (PubMed:29473541). Promotes long-term memory by phosphorylating meng and by regulating CrebB protein stability and activity (PubMed:29473541). As part of ethanol response in the glia, mediates ethanol-induced structural remodeling of actin cytoskeleton and perineurial membrane topology when anchored to the membrane (PubMed:29444420).
(UniProt, P12370)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Cos: Costal (P. Simpson)
Mirror-image duplications of wings and halteres; duplications of wing begin in mid-costal region and may include entire anterior compartment; posterior compartments unaffected, even when transformed into anterior compartments in en homozygotes. Penetrance of Cos higher when maternally than when paternally inherited. Class I alleles are homozygous viable and display pattern duplications in both heterozygotes and homozygotes. Penetrance and expressivity variable and may overlap wild type. Class II alleles are lethal when homozygous or in heteroallelic combination with one another; they sometimes display pattern duplications in heterozygotes. Class III alleles are homozygous lethal and have no dominant phenotype. Flies heterozygous for alleles of any of the three classes display the phenotype when simultaneously heterozygous for lethal alleles or deletions of cos; the heterozygous expression of Cos is dependent on the number of cos+ alleles present, with the severity of wing duplication decreasing as the number of cos+ alleles increases from one to three. Flies heterozygous for Cos and homozygous or hemizygous for some viable alleles of cos die as pharate adults and display pattern duplications in the anterior compartment of every body segment. Homozygotes of class II and IIIK alleles die as embryos with abnormal cuticular patterns; failure to develop of variable extents of the anterior end of the embryo, i.e. head or head and thorax, as well as mirror image duplications of anterior denticle belts of abdominal segments, or more frequently simply disturbed denticle polarity. Embryos that are simultaneously homozygous for cosV alleles and Cos3 are more severely affected, with some exhibiting a bicaudal phenotype. cosV1 Cos2/Df(2R)CA58 flies survive poorly, but show pattern duplications in the anterior compartments of all segments. Revertants of Cos are viable and wild type in phenotype; as they are presumably null alleles, Cos mutations are presumed to be gain of function alleles.
Pka-C1
Encodes one of the isoforms of the catalytic subunit form of Pka.
Summary (Interactive Fly)
catalytic subunit of PKA - involved in Hedgehog signaling pathway and functions in learning in the adult - contributes to axis specification, rhythmic behavior and synaptic transmission
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\Pka-C1 or the JBrowse view of Dmel\Pka-C1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0079851
5114
353
FBtr0079852
5312
353
FBtr0335495
4480
353
Additional Transcript Data and Comments
Reported size (kB)
5.1, 4.1, 3.4 (northern blot)
5.1, 4.1, 3.4, 2.4 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0079448
40.8
353
9.04
FBpp0089382
40.8
353
9.04
FBpp0307466
40.8
353
9.04
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

353 aa isoforms: Pka-C1-PB, Pka-C1-PC, Pka-C1-PD
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)
Composed of two regulatory chains and two catalytic chains.
(UniProt, P12370)
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pka-C1 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Gene Ontology (38 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from physical interaction with FLYBASE:slo; FB:FBgn0003429
inferred from direct assay
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR039083
(assigned by InterPro )
inferred from electronic annotation with InterPro:IPR039083
(assigned by InterPro )
Biological Process (28 terms)
Terms Based on Experimental Evidence (23 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from genetic interaction with FLYBASE:Su(fu); FB:FBgn0005355
inferred from direct assay
inferred from genetic interaction with FLYBASE:smo; FB:FBgn0003444
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from genetic interaction with UniProtKB:Q9VM90
(assigned by UniProt )
inferred from direct assay
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (6 terms)
CV Term
Evidence
References
non-traceable author statement
inferred from electronic annotation with InterPro:IPR039083
(assigned by InterPro )
traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000682577
(assigned by GO_Central )
Cellular Component (5 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000682577
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Pka-C1 transcripts are observed during oogenesis. Transcripts are first observed in the germarium, are present at slightly higher levels in the oocyte relative to nurse cells in stages 5-6 and are sometimes transiently anteriorly localized at stage 8.
Pka-C1 transcripts are detected predominantly in the perikarya of the mushroom bodies. Weak signal is observed over the cortex of the central brain, optic lobes, and thoracic ganglia.
A strong Pka-C1 transcript of 5.1kb is observed in adults. Weaker transcripts of 4.1kb and 3.4kb are also observed.
Pka-C1 transcripts are detected at all stages on northern blots. The relative abundance of the various Pka-C1 transcripts varies through development. The longer transcripts are particularly abundant in adults and the shorter transcripts are most abundant in embryos.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
enzyme assay or biochemical detection
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Pka-C1 protein is detected in all parts of all Kenyon cells in freshly eclosed adults.
Expression of Pka-C1 is within the presumptive mushroom body neuroblasts.
Pka-C1 activities were measured in a number of larval and adult tissues. Levels are highest in the larval brain and male accessory glands also show high levels of activity. Pka-C1 activity is also detected in testis, ovary, and wing disc.
Pka-C1 protein is strongly expressed in the mushroom body alpha, beta and gamma lobes, spur and calyx. It is moderately expressed in the mushroom body alpha' and beta' lobes.
Pka-C1 protein is associated with germ cell membranes throughout oogenesis.
Pka-C1 protein is preferentially expressed in mushroom body neurons in adult heads.
Pka-C1 protein activity is detected at all developmental stages.
Marker for
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
Reporter: P{lacW}Pka-C1s4402
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{PZ}Pka-C101272
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{PZ}Pka-C106353
Stage
Tissue/Position (including subcellular localization)
Reference
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Pka-C1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 77 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 23 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Pka-C1
Transgenic constructs containing regulatory region of Pka-C1
Deletions and Duplications ( 30 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
mesothoracic tergum & macrochaeta | somatic clone | cell non-autonomous
oocyte & microtubule | germ-line clone
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (6)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
14 of 15
Yes
Yes
11 of 15
No
Yes
4 of 15
No
No
1 of 15
No
No
 
1 of 15
No
No
 
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
13 of 15
No
Yes
4 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (5)
11 of 13
Yes
Yes
11 of 13
Yes
Yes
2 of 13
No
No
1 of 13
No
Yes
1 of 13
No
No
 
Xenopus tropicalis (Western clawed frog) (20)
10 of 12
Yes
Yes
6 of 12
No
Yes
2 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
Danio rerio (Zebrafish) (11)
13 of 15
Yes
Yes
13 of 15
Yes
Yes
9 of 15
No
Yes
9 of 15
No
Yes
4 of 15
No
No
2 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
14 of 15
Yes
Yes
4 of 15
No
No
Arabidopsis thaliana (thale-cress) (1)
1 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (4)
11 of 15
Yes
Yes
9 of 15
No
Yes
9 of 15
No
Yes
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (1)
7 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091909ZG )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091506EB )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Musca domestica
House fly
Musca domestica
House fly
Musca domestica
House fly
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles darlingi
American malaria mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Anopheles gambiae
Malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W06IH )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X06E7 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G10O8 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (7)
6 of 10
6 of 10
6 of 10
3 of 10
3 of 10
3 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 2 )
    Modifiers Based on Experimental Evidence ( 7 )
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Composed of two regulatory chains and two catalytic chains.
    (UniProt, P12370 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    Negative Regulators of Hedgehog Signaling Pathway -
    Negative regulators of hedgehog signaling down-regulation the pathway, resulting in the repression of transcription of hh-responsive genes.
    Positive Regulators of Hedgehog Signaling Pathway -
    Positive regulators of hedgehog signaling down-regulation the pathway, enhancing the activation of transcription of hh-responsive genes.
    External Data
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-36
    Cytogenetic map
    Sequence location
    2L:9,682,315..9,699,297 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    30C5-30C5
    Limits computationally determined from genome sequence between P{EP}EP361&P{EP}peloEP2160 and P{lacW}zf30Ck02506&P{EP}undEP424
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    30C1-30C2
    (determined by in situ hybridisation)
    30C-30C
    (determined by in situ hybridisation)
    30C1-30C6
    (determined by in situ hybridisation) 30C (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Pka-C1Cos-9, Pka-C1Cos-2, Pka-C1Cos-3 and Pka-C1Cos-8 map near 30C by recombination mapping.
    Stocks and Reagents
    Stocks (35)
    Genomic Clones (27)
    cDNA Clones (101)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for database merge of
    Additional comments
    A number of alleles previously assigned to the "Cos" locus actually map to two separate genes; Pka-C1 and Pka-R2.
    Other Comments
    Pka-C1 is necessary for smo phosphorylation and stabilization.
    Pka-C1 is necessary for the death and resorption of wing blade cells after eclosion.
    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
    2 alleles of Pka-C1 been recovered in a screen for mutations with mutant phenotypes in clones in the wing.
    Pka-C1 appears to have two distinct roles in hh signal transduction, nuclear or cytoplasmic; or may inhibit a possible function of free Pka-R1 in signal transduction.
    PKA inhibits the activity of full-length ci protein in addition to its role in regulating ci proteolysis.
    Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.
    Identification: Enhancer trap screen designed to discover genes involved in the cellular aspects of defense mechanisms, as well as in melanotic tumor formation processes linked to blood cell disregulation.
    Each primordia of the genital disc (female genital, male genital and anal primordia) is divided into anterior and posterior compartments. Genes known to be expressed in compartment-specific manner in discs are expressed in analogous patterns in each primordia.
    Pka-C1 plays a critical role in the flow of temporal information from circadian pacemaker cells to specific downstream targets controlling overt behaviours.
    The temporal regulation of per protein and RNA products is used to evaluate the status of the oscillatory mechanism in Pka-C1 mutants and to determine the site of action on the circadian timing system that is affected by reduced levels of Pka-C1.
    Type II cAMP-dependent protein kinase (PKAII) (composed of Pka-R2 and Pka-C1 subunits) is restricted to neuronal tissue, in which it accounts for more than 90% of total PKA activity.
    Nf1 and Pka-C1 interact in a pathway that controls the overall growth of flies.
    Pka-C1 is involved in medium-term memory formation.
    The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation. Pka-C1 is required for germ cell viability or early oogenesis.
    Results from cell culture studies suggest that fu and Pka-C1 function downstream of hh but in parallel pathways that eventually converge distal to fu.
    Pka-C1 and hh have antagonistic effects on a common substrate which regulates transcription of dpp and wg.
    Pka-C1 is essential during limb development to prevent inappropriate dpp and wg expression. A constitutively active form of Mmus\Pkaca, can prevent inappropriate dpp and wg expression but does not interfere with their normal induction by hh. The basal activity of Pka-C1 imposes a block on the transcription of dpp and wg and hh exerts its organizing influence by alleviating the block.
    Pka-C1 may be an essential component of the signal transduction pathway through which ptc regulates dpp and wg in the imaginal discs. Removing Pka-C1 activity is largely equivalent to reducing ptc activity.
    Pka-C1 is a component of the signal transduction system through which cell interactions control pattern formation in the developing imaginal discs.
    Pka-C1 activity is not regulated by ptc but may be regulated by hh.
    Pka-C1 is an integral component of the mechanism that restricts the expression of dpp, ptc and wg in imaginal discs.
    Pka-C1 is a component of the signalling pathway that represses dpp expression in the anterior compartment in appendage imaginal discs and anterior to the morphogenetic furrow in eye discs.
    Pka-C1 is a key component of a signal transduction pathway that regulates dpp expression in the appendage and eye discs. Pka-C1 is a repressor of dpp expression in the anterior compartment of the wing and leg discs and in cells anterior to the morphogenetic furrow in the eye disc.
    hh pathway mutants induce ectopic morphogenetic furrows. Results show that ommatidial clusters are self-organising units whose polarity in one axis is determined by the direction of furrow progression and which can independently define the position of an equator without reference to the global coordinates of the eye disc.
    Mutations in ptc show strong-non-autonomous effects in clones induced in the developing eye. Both ptc and Pka-C1 are required for the correct regulation of morphogenetic furrow progression, apparently via repression of dpp. Loss of function of either ptc or Pka-C1 in cells anterior to the furrow results in an ectopic furrow characterised by non-autonomous propagation of dpp expression outside the mutant tissue and ectopic photoreceptor differentiation. Both ptc and Pka-C1 act downstream of hh.
    Pka-C1 is required for the correct spatial regulation of dpp expression during eye development. Loss of Pka-C1 function is sufficient to produce an ectopic morphogenetic wave marked by premature ectopic photoreceptor differentiation and non-autonomous propagation of dpp expression. Results indicate that Pka-C1 lies in a signalling pathway that controls the orderly temporal progression of differentiation across the eye imaginal disc.
    Mutations of Pka-C1 cause similar mislocalisations of bcd and osk RNAs to those observed from N mutations. Mutations also severely disrupt the organisation of microtubules at the posterior of the oocyte at the time of bcd and osk localisation.
    Pka-C1 is required for normal adult rhythms.
    Activation of endogenous Pka-C1, expression of wild type Tl receptors or treatment of cells with activators of Pkc53E and radical oxygen intermediates have only a marginal effects on the cellular distribution of dl protein in culture cells.
    Pka-C1 plays a crucial role in the cAMP cascade in mushroom bodies to mediate learning and memory processes.
    Purification and characterisation of the catalytic subunit of cAMP- dependent protein kinase.
    Pole cell transplantation, genetic mosaics and isolation of conditional alleles have been used to determine the function of Pka-C1 during embryogenesis and imaginal disc development.
    The signalling pathway from Tl to dl involves Pka-C1. Nuclear transport and activation of dl results from phosphorylation of dl by the Pka-C1 product.
    Pka-C1 encodes a highly conserved catalytic subunit of the cAMP-dependent protein kinase.
    The dominant phenotype seen in flies heterozygous for the neomorphic "Cos" mutants is the result of a reduced level of function at the cos locus. Haploidy for cos enhances, whereas triploidy for cos suppresses the phenotype of "Cos" heterozygotes. Mutant embryos show phenotypes similar to those caused by mutants at the cos locus.
    Encodes one of the isoforms of the catalytic subunit form of Pka. Drosophila cAMP-dependent protein kinase has been purified from the bodies of adult flies; the enzyme is made up of catalytic and regulatory subunits (FBrf0041205). The catalytic subunit has a molecular weight of 40,000 and the regulatory subunit a molecular weight of 52,000 or 58,000 (based on electrophoretic mobilities in sodium dodecyl sulfate-polyacrylamide gels).
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 93 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    modMine - A data warehouse for the modENCODE project
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Linkouts
    ApoDroso - Functional genomic database for photoreceptor development, survival and function
    BioGRID - A database of protein and genetic interactions.
    DPiM - Drosophila Protein interaction map
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (50)
    Reported As
    Symbol Synonym
    CdkA
    Dcpk
    PKA
    (Copf et al., 2019, Giordano et al., 2018, Pan et al., 2017, Tomita et al., 2017, Zhang et al., 2015, Singh et al., 2014, Song et al., 2014, Sulkowski et al., 2014, Yamazaki et al., 2014, Wang et al., 2013, Hong et al., 2012, Wang and Jin, 2011, Terriente-Félix et al., 2010, Yamazaki et al., 2010, Kilman et al., 2009, Lebreton and Martin, 2009, Lee et al., 2009, Renault et al., 2009, Tripodi et al., 2008, Bangi et al., 2007, Davis et al., 2007, Dolezelova et al., 2007, Price et al., 2007, Walthall et al., 2007, Wentzell et al., 2007, Xu and Shen, 2007, Zhao et al., 2007, Dworkin and Gibson., 2006, Huangfu and Anderson, 2006, Smelkinson and Kalderon, 2006, Apionishev et al., 2005, Briscoe and Therond, 2005, Dawber et al., 2005, Jia et al., 2005, Kalderon, 2005, Serway et al., 2005, Steinhauer and Kalderon, 2005, Torroja et al., 2005, Zhang et al., 2005, Lum and Beachy, 2004, Ogden et al., 2004, Sanyal et al., 2004, Welte, 2004, Apionishev and Kalderon, 2003, Barnhart and Kalderon, 2003, Boudeau et al., 2003, Dubnau et al., 2003, Kimura et al., 2003, Martin and St. Johnston, 2003, Ou et al., 2003, Ou et al., 2003, Shaw and Franken, 2003, Shulman and Feany, 2003, Tong et al., 2003, van den Heuvel, 2003, Wolfgang et al., 2003, Zhang et al., 2003, Jiang, 2002, Kalderon, 2002, Kuwabara and Labouesse, 2002, Lefers and Holmgren, 2002, Rothenfluh and Heberlein, 2002, Wickens et al., 2002, Burrell and Sahley, 2001, Coleman and Neckameyer, 2001, Ingham and McMahon, 2001, Johnstone and Lasko, 2001, Lawrence, 2001, Patel et al., 2001, Rodan et al., 2001, Venkatesh et al., 2001, Zhu et al., 2001, Brill et al., 2000, Jans et al., 2000, Methot and Basler, 2000, Milan and Cohen, 2000, Price and Kalderon, 2000, Vervoort, 2000, Zhang and Kalderon, 2000, Baum et al., 1999, Chen et al., 1999, Marsh and Theisen, 1999, Matise and Joyner, 1999, Monnier et al., 1999, Phillips et al., 1999, Ruiz i Altaba, 1999, Wang and Holmgren, 1999, Wolfgang et al., 1999, Goodrich and Scott, 1998, Ingham, 1998, Johnston and Scott, 1998, Belvin and Yin, 1997, Burke and Basler, 1997, Cadigan and Nusse, 1997, Cooperstock and Lipshitz, 1997, Hammerschmidt et al., 1997, Ingham, 1997, Jackson et al., 1997, Johnson and Scott, 1997, Robinson and Cooley, 1997, Rodriguez and Basler, 1997, Ruiz i Altaba, 1997, Dong and Krause, 1996, Heberlein et al., 1996, Li et al., 1996, Norris and Manley, 1996, Nusse, 1996, Ray and Schupbach, 1996, van den Heuvel and Ingham, 1996, van den Heuvel and Ingham, 1996, Wiersdorff et al., 1996, Blair, 1995, Johnson et al., 1995, Kalderon, 1995, Lasko, 1995, Majercak et al., 1995, Micklem, 1995, Lane and Kalderon, 1994, Kubota et al., 1993, Aszodi et al., 1991, Drain et al., 1991)
    l(2)cos1
    l(2)s4402
    Name Synonyms
    PKA catalytic subunit
    Protein kinase, cAMP-dependent, catalytic subunit 1
    cAMP-dependent protein kinase
    catalytic subunit of cAMP-dependent protein kinase
    costal-1
    Secondary FlyBase IDs
    • FBgn0000353
    • FBgn0010695
    Datasets (0)
    Study focus (0)
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    References (533)