Gene Dmel\Pka-C1

30C1-30C6

(determined by in situ hybridisation) 30C (determined by in situ hybridisation)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Pka-C1^Cos-9, Pka-C1^Cos-2, Pka-C1^Cos-3 and Pka-C1^Cos-8 map near 30C by recombination mapping.

(Collier et al., 2004)

Gene Model & Products

Please see the GBrowse view of Dmel\Pka-C1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.47

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Pka-C1-RB

FBtr0079851

NM_164866

5114

353

Pka-C1-RC

FBtr0079852

NM_205950

5312

353

Pka-C1-RD

FBtr0335495

NM_057629

4480

353

Additional Transcript Data & Comments

Reported size (kB)

5.1, 4.1, 3.4 (northern blot)

5.1, 4.1, 3.4, 2.4 (northern blot)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Pka-C1-PB

FBpp0079448

40.8

353

9.04

NP_723479

AAN10703

Pka-C1-PC

FBpp0089382

40.8

353

9.04

NP_995672

AAS64669

Pka-C1-PD

FBpp0307466

40.8

353

9.04

NP_476977

AAF52797

Additional Polypeptide Data & Comments

Reported size (kDa)

353 (aa)

352 (aa)

40 (kD observed)

(Foster et al., 1984)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Protein kinase, catalytic domain (IPR000719)

AGC-kinase, C-terminal (IPR000961)

Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290)

Serine/threonine-protein kinase, active site (IPR008271)

Protein kinase-like domain (IPR011009)

Protein kinase, ATP binding site (IPR017441)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P12370

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

(Fisher et al., 2012)

Comment:maternally deposited

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

adult stage

optic lobe

thoracico-abdominal ganglion

adult brain

adult mushroom body

adult stage & oogenesis

germarium

oocyte

nurse cell

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

adult stage

Additional Descriptive Data

Pka-C1 transcripts are detected predominantly in the perikarya of the mushroom bodies. Weak signal is observed over the cortex of the central brain, optic lobes, and thoracic ganglia.

Pka-C1 transcripts are observed during oogenesis. Transcripts are first observed in the germarium, are present at slightly higher levels in the oocyte relative to nurse cells in stages 5-6 and are sometimes transiently anteriorly localized at stage 8.

Pka-C1 transcripts are detected at all stages on northern blots. The relative abundance of the various Pka-C1 transcripts varies through development. The longer transcripts are particularly abundant in adults and the shorter transcripts are most abundant in embryos.

A strong Pka-C1 transcript of 5.1kb is observed in adults. Weaker transcripts of 4.1kb and 3.4kb are also observed.

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

enzyme assay or biochemical detection

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Foster et al., 1984)

third instar larval stage

embryonic/larval brain

wing disc

adult stage | male

testis

male accessory gland

adult stage | female

ovary

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16

mushroom body neuroblast

(Noveen et al., 2000)

adult stage

mushroom body beta-lobe

mushroom body calyx

mushroom body alpha-lobe

mushroom body alpha-lobe | intense

mushroom body beta-lobe | intense

mushroom body spur | intense

calyx of adult mushroom body | intense

adult mushroom body

adult mushroom body beta'-lobe

adult mushroom body gamma-lobe

adult mushroom body gamma-lobe | intense

adult mushroom body alpha'-lobe

Additional Descriptive Data

Pka-C1 protein is associated with germ cell membranes throughout oogenesis.

Expression of Pka-C1 is within the presumptive mushroom body neuroblasts.

(Noveen et al., 2000)

Pka-C1 protein activity is detected at all developmental stages.

(Foster et al., 1984)

Pka-C1 protein is strongly expressed in the mushroom body alpha, beta and gamma lobes, spur and calyx. It is moderately expressed in the mushroom body alpha' and beta' lobes.

Pka-C1 protein is preferentially expressed in mushroom body neurons in adult heads.

Pka-C1 activities were measured in a number of larval and adult tissues. Levels are highest in the larval brain and male accessory glands also show high levels of activity. Pka-C1 activity is also detected in testis, ovary, and wing disc.

Marker for

Subcellular Localization (GO Cellular Component)

CV term

(Lane and Kalderon, 1995, Khanna et al., 2010)

References

plasma membrane

inferred from direct assay

Expression Deduced from Reporters

Reporter: P{lacW}Pka-C1^s4402

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval hindgut | restricted

Reporter: P{PZ}Pka-C1⁰¹²⁷²

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval brain | presumptive

anal pad

embryonic/larval midgut

embryonic/larval somatic muscle | restricted

embryonic/larval posterior spiracle

gastric caecum

embryonic/larval anterior spiracle

presumptive embryonic/larval peripheral nervous system

embryonic/larval hindgut

wandering third instar larval stage

epidermis

Malpighian tubule

embryonic/larval hindgut

embryonic/larval trachea

embryonic/larval foregut

embryonic/larval somatic muscle

embryonic/larval midgut

embryonic/larval cuticle

embryonic/larval salivary gland

adult stage

adult brain

(Vosshall, 1998.8.10)

Reporter: P{PZ}Pka-C1⁰⁶³⁵³

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

prothoracic gland

wandering third instar larval stage

embryonic/larval optic lobe

ventral nerve cord

eye disc

(The modENCODE Consortium, 2010, Graveley et al., 2011)

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, larval/adult salivary gland, larval trachea, adult ovary, larval carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		23
central nervous system, larvae L3		115
central nervous system, pupae P8		172
head, virgin 1-day female		77
head, virgin 4-day female		63
head, virgin 20-day female		34
head, mated 1-day female		73
head, mated 4-day female		56
head, mated 20-day female		37
head, mated 1-day male		104
head, mated 4-day male		99
head, mated 20-day male		77
salivary gland, larvae L3 wandering		12
salivary gland, white prepupae		69
digestive system, larvae L3 wandering		21
digestive system, 1-day adult		22
digestive system, 4-day adult		30
digestive system, 20-day adult		20
fat body, larvae L3 wandering		9
fat body, white prepupae		20
fat body, pupae P8		42
carcass, larvae L3 wandering		50
carcass, 1-day adult		41
carcass, 4-day adult		39
carcass, 20-day adult		29
ovary, virgin 4-day female		42
ovary, mated 4-day female		45
testis, mated 4-day male		3
accessory gland, mated 4-day male		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		23
central nervous system, larvae L3		(115)
central nervous system, pupae P8		(172)
head, virgin 1-day female		(77)
head, virgin 4-day female		(63)
head, virgin 20-day female		(34)
head, mated 1-day female		(73)
head, mated 4-day female		(56)
head, mated 20-day female		(37)
head, mated 1-day male		(104)
head, mated 4-day male		(99)
head, mated 20-day male		(77)
salivary gland, larvae L3 wandering		12
salivary gland, white prepupae		(69)
digestive system, larvae L3 wandering		21
digestive system, 1-day adult		22
digestive system, 4-day adult		(30)
digestive system, 20-day adult		20
fat body, larvae L3 wandering		9
fat body, white prepupae		20
fat body, pupae P8		(42)
carcass, larvae L3 wandering		(50)
carcass, 1-day adult		(41)
carcass, 4-day adult		(39)
carcass, 20-day adult		(29)
ovary, virgin 4-day female		(42)
ovary, mated 4-day female		(45)
testis, mated 4-day male		3
accessory gland, mated 4-day male		14
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		23
central nervous system, larvae L3		(115)
central nervous system, pupae P8		(172)
head, virgin 1-day female		77
head, virgin 4-day female		63
head, virgin 20-day female		34
head, mated 1-day female		73
head, mated 4-day female		56
head, mated 20-day female		37
head, mated 1-day male		104
head, mated 4-day male		99
head, mated 20-day male		77
salivary gland, larvae L3 wandering		12
salivary gland, white prepupae		69
digestive system, larvae L3 wandering		21
digestive system, 1-day adult		22
digestive system, 4-day adult		30
digestive system, 20-day adult		20
fat body, larvae L3 wandering		9
fat body, white prepupae		20
fat body, pupae P8		42
carcass, larvae L3 wandering		50
carcass, 1-day adult		41
carcass, 4-day adult		39
carcass, 20-day adult		29
ovary, virgin 4-day female		42
ovary, mated 4-day female		45
testis, mated 4-day male		3
accessory gland, mated 4-day male		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		23
central nervous system, larvae L3		115
central nervous system, pupae P8		172
head, virgin 1-day female		77
head, virgin 4-day female		63
head, virgin 20-day female		34
head, mated 1-day female		73
head, mated 4-day female		56
head, mated 20-day female		37
head, mated 1-day male		104
head, mated 4-day male		99
head, mated 20-day male		77
salivary gland, larvae L3 wandering		12
salivary gland, white prepupae		69
digestive system, larvae L3 wandering		21
digestive system, 1-day adult		22
digestive system, 4-day adult		30
digestive system, 20-day adult		20
fat body, larvae L3 wandering		9
fat body, white prepupae		20
fat body, pupae P8		42
carcass, larvae L3 wandering		50
carcass, 1-day adult		41
carcass, 4-day adult		39
carcass, 20-day adult		29
ovary, virgin 4-day female		42
ovary, mated 4-day female		45
testis, mated 4-day male		3
accessory gland, mated 4-day male		14
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		23
central nervous system, larvae L3		115
central nervous system, pupae P8		172
head, virgin 1-day female		77
head, virgin 4-day female		63
head, virgin 20-day female		34
head, mated 1-day female		73
head, mated 4-day female		56
head, mated 20-day female		37
head, mated 1-day male		104
head, mated 4-day male		99
head, mated 20-day male		77
salivary gland, larvae L3 wandering		12
salivary gland, white prepupae		69
digestive system, larvae L3 wandering		21
digestive system, 1-day adult		22
digestive system, 4-day adult		30
digestive system, 20-day adult		20
fat body, larvae L3 wandering		9
fat body, white prepupae		20
fat body, pupae P8		42
carcass, larvae L3 wandering		50
carcass, 1-day adult		41
carcass, 4-day adult		39
carcass, 20-day adult		29
ovary, virgin 4-day female		42
ovary, mated 4-day female		45
testis, mated 4-day male		3
accessory gland, mated 4-day male		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		23
central nervous system, larvae L3		(115)
central nervous system, pupae P8		(172)
head, virgin 1-day female		(77)
head, virgin 4-day female		(63)
head, virgin 20-day female		(34)
head, mated 1-day female		(73)
head, mated 4-day female		(56)
head, mated 20-day female		(37)
head, mated 1-day male		(104)
head, mated 4-day male		(99)
head, mated 20-day male		(77)
salivary gland, larvae L3 wandering		12
salivary gland, white prepupae		(69)
digestive system, larvae L3 wandering		21
digestive system, 1-day adult		22
digestive system, 4-day adult		30
digestive system, 20-day adult		20
fat body, larvae L3 wandering		9
fat body, white prepupae		20
fat body, pupae P8		(42)
carcass, larvae L3 wandering		(50)
carcass, 1-day adult		(41)
carcass, 4-day adult		(39)
carcass, 20-day adult		29
ovary, virgin 4-day female		(42)
ovary, mated 4-day female		(45)
testis, mated 4-day male		3
accessory gland, mated 4-day male		14
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		23
central nervous system, larvae L3		115
central nervous system, pupae P8		(172)
head, virgin 1-day female		77
head, virgin 4-day female		63
head, virgin 20-day female		34
head, mated 1-day female		73
head, mated 4-day female		56
head, mated 20-day female		37
head, mated 1-day male		104
head, mated 4-day male		99
head, mated 20-day male		77
salivary gland, larvae L3 wandering		12
salivary gland, white prepupae		69
digestive system, larvae L3 wandering		21
digestive system, 1-day adult		22
digestive system, 4-day adult		30
digestive system, 20-day adult		20
fat body, larvae L3 wandering		9
fat body, white prepupae		20
fat body, pupae P8		42
carcass, larvae L3 wandering		50
carcass, 1-day adult		41
carcass, 4-day adult		39
carcass, 20-day adult		29
ovary, virgin 4-day female		42
ovary, mated 4-day female		45
testis, mated 4-day male		3
accessory gland, mated 4-day male		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		23
central nervous system, larvae L3		115
central nervous system, pupae P8		172
head, virgin 1-day female		77
head, virgin 4-day female		63
head, virgin 20-day female		34
head, mated 1-day female		73
head, mated 4-day female		56
head, mated 20-day female		37
head, mated 1-day male		104
head, mated 4-day male		99
head, mated 20-day male		77
salivary gland, larvae L3 wandering		12
salivary gland, white prepupae		69
digestive system, larvae L3 wandering		21
digestive system, 1-day adult		22
digestive system, 4-day adult		30
digestive system, 20-day adult		20
fat body, larvae L3 wandering		9
fat body, white prepupae		20
fat body, pupae P8		42
carcass, larvae L3 wandering		50
carcass, 1-day adult		41
carcass, 4-day adult		39
carcass, 20-day adult		29
ovary, virgin 4-day female		42
ovary, mated 4-day female		45
testis, mated 4-day male		3
accessory gland, mated 4-day male		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, during late pupal stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		28
embryo 02-04hr		37
embryo 04-06hr		28
embryo 06-08hr		22
embryo 08-10hr		25
embryo 10-12hr		16
embryo 12-14hr		28
embryo 14-16hr		27
embryo 16-18hr		44
embryo 18-20hr		40
embryo 20-22hr		45
embryo 22-24hr		59
larva L1		33
larva L2		31
larva L3 12hr old		29
larva L3 puffstage 1-2		18
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		26
white prepupae new		29
white prepupae 12hr		24
white prepupae 24hr		30
pupae 2d postWPP		32
pupae 3d postWPP		56
pupae 4d postWPP		24
adult male 01day		29
adult male 05day		22
adult male 30day		16
adult female 01day		19
adult female 05day		23
adult female 30day		20
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(28)
embryo 02-04hr		(37)
embryo 04-06hr		(28)
embryo 06-08hr		22
embryo 08-10hr		25
embryo 10-12hr		16
embryo 12-14hr		(28)
embryo 14-16hr		(27)
embryo 16-18hr		(44)
embryo 18-20hr		(40)
embryo 20-22hr		(45)
embryo 22-24hr		(59)
larva L1		(33)
larva L2		(31)
larva L3 12hr old		(29)
larva L3 puffstage 1-2		18
larva L3 puffstage 3-6		(27)
larva L3 puffstage 7-9		26
white prepupae new		(29)
white prepupae 12hr		24
white prepupae 24hr		(30)
pupae 2d postWPP		(32)
pupae 3d postWPP		(56)
pupae 4d postWPP		24
adult male 01day		(29)
adult male 05day		22
adult male 30day		16
adult female 01day		19
adult female 05day		23
adult female 30day		20
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		28
embryo 02-04hr		37
embryo 04-06hr		28
embryo 06-08hr		22
embryo 08-10hr		25
embryo 10-12hr		16
embryo 12-14hr		28
embryo 14-16hr		27
embryo 16-18hr		44
embryo 18-20hr		40
embryo 20-22hr		45
embryo 22-24hr		59
larva L1		33
larva L2		31
larva L3 12hr old		29
larva L3 puffstage 1-2		18
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		26
white prepupae new		29
white prepupae 12hr		24
white prepupae 24hr		30
pupae 2d postWPP		32
pupae 3d postWPP		56
pupae 4d postWPP		24
adult male 01day		29
adult male 05day		22
adult male 30day		16
adult female 01day		19
adult female 05day		23
adult female 30day		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		28
embryo 02-04hr		37
embryo 04-06hr		28
embryo 06-08hr		22
embryo 08-10hr		25
embryo 10-12hr		16
embryo 12-14hr		28
embryo 14-16hr		27
embryo 16-18hr		44
embryo 18-20hr		40
embryo 20-22hr		45
embryo 22-24hr		59
larva L1		33
larva L2		31
larva L3 12hr old		29
larva L3 puffstage 1-2		18
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		26
white prepupae new		29
white prepupae 12hr		24
white prepupae 24hr		30
pupae 2d postWPP		32
pupae 3d postWPP		56
pupae 4d postWPP		24
adult male 01day		29
adult male 05day		22
adult male 30day		16
adult female 01day		19
adult female 05day		23
adult female 30day		20
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		28
embryo 02-04hr		37
embryo 04-06hr		28
embryo 06-08hr		22
embryo 08-10hr		25
embryo 10-12hr		16
embryo 12-14hr		28
embryo 14-16hr		27
embryo 16-18hr		44
embryo 18-20hr		40
embryo 20-22hr		45
embryo 22-24hr		59
larva L1		33
larva L2		31
larva L3 12hr old		29
larva L3 puffstage 1-2		18
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		26
white prepupae new		29
white prepupae 12hr		24
white prepupae 24hr		30
pupae 2d postWPP		32
pupae 3d postWPP		56
pupae 4d postWPP		24
adult male 01day		29
adult male 05day		22
adult male 30day		16
adult female 01day		19
adult female 05day		23
adult female 30day		20
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		28
embryo 02-04hr		(37)
embryo 04-06hr		28
embryo 06-08hr		22
embryo 08-10hr		25
embryo 10-12hr		16
embryo 12-14hr		28
embryo 14-16hr		27
embryo 16-18hr		(44)
embryo 18-20hr		(40)
embryo 20-22hr		(45)
embryo 22-24hr		(59)
larva L1		(33)
larva L2		31
larva L3 12hr old		29
larva L3 puffstage 1-2		18
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		26
white prepupae new		29
white prepupae 12hr		24
white prepupae 24hr		30
pupae 2d postWPP		32
pupae 3d postWPP		(56)
pupae 4d postWPP		24
adult male 01day		29
adult male 05day		22
adult male 30day		16
adult female 01day		19
adult female 05day		23
adult female 30day		20
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		28
embryo 02-04hr		37
embryo 04-06hr		28
embryo 06-08hr		22
embryo 08-10hr		25
embryo 10-12hr		16
embryo 12-14hr		28
embryo 14-16hr		27
embryo 16-18hr		44
embryo 18-20hr		40
embryo 20-22hr		45
embryo 22-24hr		59
larva L1		33
larva L2		31
larva L3 12hr old		29
larva L3 puffstage 1-2		18
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		26
white prepupae new		29
white prepupae 12hr		24
white prepupae 24hr		30
pupae 2d postWPP		32
pupae 3d postWPP		56
pupae 4d postWPP		24
adult male 01day		29
adult male 05day		22
adult male 30day		16
adult female 01day		19
adult female 05day		23
adult female 30day		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		28
embryo 02-04hr		37
embryo 04-06hr		28
embryo 06-08hr		22
embryo 08-10hr		25
embryo 10-12hr		16
embryo 12-14hr		28
embryo 14-16hr		27
embryo 16-18hr		44
embryo 18-20hr		40
embryo 20-22hr		45
embryo 22-24hr		59
larva L1		33
larva L2		31
larva L3 12hr old		29
larva L3 puffstage 1-2		18
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		26
white prepupae new		29
white prepupae 12hr		24
white prepupae 24hr		30
pupae 2d postWPP		32
pupae 3d postWPP		56
pupae 4d postWPP		24
adult male 01day		29
adult male 05day		22
adult male 30day		16
adult female 01day		19
adult female 05day		23
adult female 30day		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		23
Schneider line 2 Sg4		10
embryonic 1182-4H		19
embryonic GM2		22
embryonic Kc167		11
embryonic S1		20
embryonic S3		24
leg disc CME L1		14
wing disc CME-W2		13
wing disc ML-DmD8		18
wing disc ML-DmD9		14
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		6
wing disc ML-DmD32		13
haltere disc ML-DmD17-c3		32
eye-antennal disc ML-DmD11		18
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		35
CNS ML-DmBG2-c2		19
tumorous blood cells mbn2		21
ovary fGS/OSS		13
ovary OSC		17
ovary OSS		20
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		23
Schneider line 2 Sg4		10
embryonic 1182-4H		19
embryonic GM2		22
embryonic Kc167		11
embryonic S1		20
embryonic S3		24
leg disc CME L1		14
wing disc CME-W2		13
wing disc ML-DmD8		18
wing disc ML-DmD9		14
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		6
wing disc ML-DmD32		13
haltere disc ML-DmD17-c3		(32)
eye-antennal disc ML-DmD11		18
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		(35)
CNS ML-DmBG2-c2		19
tumorous blood cells mbn2		21
ovary fGS/OSS		13
ovary OSC		17
ovary OSS		20
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		23
Schneider line 2 Sg4		10
embryonic 1182-4H		19
embryonic GM2		22
embryonic Kc167		11
embryonic S1		20
embryonic S3		24
leg disc CME L1		14
wing disc CME-W2		13
wing disc ML-DmD8		18
wing disc ML-DmD9		14
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		6
wing disc ML-DmD32		13
haltere disc ML-DmD17-c3		32
eye-antennal disc ML-DmD11		18
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		35
CNS ML-DmBG2-c2		19
tumorous blood cells mbn2		21
ovary fGS/OSS		13
ovary OSC		17
ovary OSS		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		23
Schneider line 2 Sg4		10
embryonic 1182-4H		19
embryonic GM2		22
embryonic Kc167		11
embryonic S1		20
embryonic S3		24
leg disc CME L1		14
wing disc CME-W2		13
wing disc ML-DmD8		18
wing disc ML-DmD9		14
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		6
wing disc ML-DmD32		13
haltere disc ML-DmD17-c3		32
eye-antennal disc ML-DmD11		18
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		35
CNS ML-DmBG2-c2		19
tumorous blood cells mbn2		21
ovary fGS/OSS		13
ovary OSC		17
ovary OSS		20
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		23
Schneider line 2 Sg4		10
embryonic 1182-4H		19
embryonic GM2		22
embryonic Kc167		11
embryonic S1		20
embryonic S3		24
leg disc CME L1		14
wing disc CME-W2		13
wing disc ML-DmD8		18
wing disc ML-DmD9		14
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		6
wing disc ML-DmD32		13
haltere disc ML-DmD17-c3		32
eye-antennal disc ML-DmD11		18
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		35
CNS ML-DmBG2-c2		19
tumorous blood cells mbn2		21
ovary fGS/OSS		13
ovary OSC		17
ovary OSS		20
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		23
Schneider line 2 Sg4		10
embryonic 1182-4H		19
embryonic GM2		22
embryonic Kc167		11
embryonic S1		20
embryonic S3		24
leg disc CME L1		14
wing disc CME-W2		13
wing disc ML-DmD8		18
wing disc ML-DmD9		14
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		6
wing disc ML-DmD32		13
haltere disc ML-DmD17-c3		32
eye-antennal disc ML-DmD11		18
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		(35)
CNS ML-DmBG2-c2		19
tumorous blood cells mbn2		21
ovary fGS/OSS		13
ovary OSC		17
ovary OSS		20
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		23
Schneider line 2 Sg4		10
embryonic 1182-4H		19
embryonic GM2		22
embryonic Kc167		11
embryonic S1		20
embryonic S3		24
leg disc CME L1		14
wing disc CME-W2		13
wing disc ML-DmD8		18
wing disc ML-DmD9		14
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		6
wing disc ML-DmD32		13
haltere disc ML-DmD17-c3		32
eye-antennal disc ML-DmD11		18
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		35
CNS ML-DmBG2-c2		19
tumorous blood cells mbn2		21
ovary fGS/OSS		13
ovary OSC		17
ovary OSS		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		23
Schneider line 2 Sg4		10
embryonic 1182-4H		19
embryonic GM2		22
embryonic Kc167		11
embryonic S1		20
embryonic S3		24
leg disc CME L1		14
wing disc CME-W2		13
wing disc ML-DmD8		18
wing disc ML-DmD9		14
wing disc ML-DmD16-c3		22
wing disc ML-DmD21		6
wing disc ML-DmD32		13
haltere disc ML-DmD17-c3		32
eye-antennal disc ML-DmD11		18
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		35
CNS ML-DmBG2-c2		19
tumorous blood cells mbn2		21
ovary fGS/OSS		13
ovary OSC		17
ovary OSS		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		25
cold shock, 4-day adult		21
heat shock, 4-day adult		29
Cadmium 50 mM 6 hrs, larvae L3		22
Cadmium 50 mM 12 hrs, larvae L3		30
Cadmium 50 mM 48 hrs, 4-day adult		38
Cadmium 100 mM 48 hrs, 4-day adult		39
Copper 0.5 mM 12 hrs, larvae L3		24
Copper 15 mM 48 hrs, 4-day adult		79
Zinc 5 mM 12 hrs, larvae L3		35
Zinc 4.5 mM 48 hrs, 4-day adult		48
Ethanol 2.5% 3 hrs, larvae L3		26
Ethanol 5% 3 hrs, larvae L3		26
Ethanol 10% 3 hrs, larvae L3		28
Caffeine 1.5 mg/ml 4 hrs, larvae L3		30
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		39
Caffeine 25 mg/ml 48 hrs, 4-day adult		28
Paraquat 5 mM 48 hrs, 4-day adult		35
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		25
Rotenone 8 μg 12 hrs, larvae L3		15
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		25
cold shock, 4-day adult		21
heat shock, 4-day adult		(29)
Cadmium 50 mM 6 hrs, larvae L3		22
Cadmium 50 mM 12 hrs, larvae L3		(30)
Cadmium 50 mM 48 hrs, 4-day adult		(38)
Cadmium 100 mM 48 hrs, 4-day adult		(39)
Copper 0.5 mM 12 hrs, larvae L3		24
Copper 15 mM 48 hrs, 4-day adult		(79)
Zinc 5 mM 12 hrs, larvae L3		(35)
Zinc 4.5 mM 48 hrs, 4-day adult		(48)
Ethanol 2.5% 3 hrs, larvae L3		26
Ethanol 5% 3 hrs, larvae L3		26
Ethanol 10% 3 hrs, larvae L3		(28)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(30)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(39)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(28)
Paraquat 5 mM 48 hrs, 4-day adult		(35)
Paraquat 10 mM 48 hrs, 4-day adult		(29)
Rotenone 2 μg 12 hrs, larvae L3		25
Rotenone 8 μg 12 hrs, larvae L3		15
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		25
cold shock, 4-day adult		21
heat shock, 4-day adult		29
Cadmium 50 mM 6 hrs, larvae L3		22
Cadmium 50 mM 12 hrs, larvae L3		30
Cadmium 50 mM 48 hrs, 4-day adult		38
Cadmium 100 mM 48 hrs, 4-day adult		39
Copper 0.5 mM 12 hrs, larvae L3		24
Copper 15 mM 48 hrs, 4-day adult		79
Zinc 5 mM 12 hrs, larvae L3		35
Zinc 4.5 mM 48 hrs, 4-day adult		48
Ethanol 2.5% 3 hrs, larvae L3		26
Ethanol 5% 3 hrs, larvae L3		26
Ethanol 10% 3 hrs, larvae L3		28
Caffeine 1.5 mg/ml 4 hrs, larvae L3		30
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		39
Caffeine 25 mg/ml 48 hrs, 4-day adult		28
Paraquat 5 mM 48 hrs, 4-day adult		35
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		25
Rotenone 8 μg 12 hrs, larvae L3		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		25
cold shock, 4-day adult		21
heat shock, 4-day adult		29
Cadmium 50 mM 6 hrs, larvae L3		22
Cadmium 50 mM 12 hrs, larvae L3		30
Cadmium 50 mM 48 hrs, 4-day adult		38
Cadmium 100 mM 48 hrs, 4-day adult		39
Copper 0.5 mM 12 hrs, larvae L3		24
Copper 15 mM 48 hrs, 4-day adult		79
Zinc 5 mM 12 hrs, larvae L3		35
Zinc 4.5 mM 48 hrs, 4-day adult		48
Ethanol 2.5% 3 hrs, larvae L3		26
Ethanol 5% 3 hrs, larvae L3		26
Ethanol 10% 3 hrs, larvae L3		28
Caffeine 1.5 mg/ml 4 hrs, larvae L3		30
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		39
Caffeine 25 mg/ml 48 hrs, 4-day adult		28
Paraquat 5 mM 48 hrs, 4-day adult		35
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		25
Rotenone 8 μg 12 hrs, larvae L3		15
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		25
cold shock, 4-day adult		21
heat shock, 4-day adult		29
Cadmium 50 mM 6 hrs, larvae L3		22
Cadmium 50 mM 12 hrs, larvae L3		30
Cadmium 50 mM 48 hrs, 4-day adult		38
Cadmium 100 mM 48 hrs, 4-day adult		39
Copper 0.5 mM 12 hrs, larvae L3		24
Copper 15 mM 48 hrs, 4-day adult		79
Zinc 5 mM 12 hrs, larvae L3		35
Zinc 4.5 mM 48 hrs, 4-day adult		48
Ethanol 2.5% 3 hrs, larvae L3		26
Ethanol 5% 3 hrs, larvae L3		26
Ethanol 10% 3 hrs, larvae L3		28
Caffeine 1.5 mg/ml 4 hrs, larvae L3		30
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		39
Caffeine 25 mg/ml 48 hrs, 4-day adult		28
Paraquat 5 mM 48 hrs, 4-day adult		35
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		25
Rotenone 8 μg 12 hrs, larvae L3		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		25
cold shock, 4-day adult		21
heat shock, 4-day adult		29
Cadmium 50 mM 6 hrs, larvae L3		22
Cadmium 50 mM 12 hrs, larvae L3		30
Cadmium 50 mM 48 hrs, 4-day adult		(38)
Cadmium 100 mM 48 hrs, 4-day adult		(39)
Copper 0.5 mM 12 hrs, larvae L3		24
Copper 15 mM 48 hrs, 4-day adult		(79)
Zinc 5 mM 12 hrs, larvae L3		(35)
Zinc 4.5 mM 48 hrs, 4-day adult		(48)
Ethanol 2.5% 3 hrs, larvae L3		26
Ethanol 5% 3 hrs, larvae L3		26
Ethanol 10% 3 hrs, larvae L3		28
Caffeine 1.5 mg/ml 4 hrs, larvae L3		30
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(39)
Caffeine 25 mg/ml 48 hrs, 4-day adult		28
Paraquat 5 mM 48 hrs, 4-day adult		(35)
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		25
Rotenone 8 μg 12 hrs, larvae L3		15
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		25
cold shock, 4-day adult		21
heat shock, 4-day adult		29
Cadmium 50 mM 6 hrs, larvae L3		22
Cadmium 50 mM 12 hrs, larvae L3		30
Cadmium 50 mM 48 hrs, 4-day adult		38
Cadmium 100 mM 48 hrs, 4-day adult		39
Copper 0.5 mM 12 hrs, larvae L3		24
Copper 15 mM 48 hrs, 4-day adult		79
Zinc 5 mM 12 hrs, larvae L3		35
Zinc 4.5 mM 48 hrs, 4-day adult		48
Ethanol 2.5% 3 hrs, larvae L3		26
Ethanol 5% 3 hrs, larvae L3		26
Ethanol 10% 3 hrs, larvae L3		28
Caffeine 1.5 mg/ml 4 hrs, larvae L3		30
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		39
Caffeine 25 mg/ml 48 hrs, 4-day adult		28
Paraquat 5 mM 48 hrs, 4-day adult		35
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		25
Rotenone 8 μg 12 hrs, larvae L3		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		25
cold shock, 4-day adult		21
heat shock, 4-day adult		29
Cadmium 50 mM 6 hrs, larvae L3		22
Cadmium 50 mM 12 hrs, larvae L3		30
Cadmium 50 mM 48 hrs, 4-day adult		38
Cadmium 100 mM 48 hrs, 4-day adult		39
Copper 0.5 mM 12 hrs, larvae L3		24
Copper 15 mM 48 hrs, 4-day adult		79
Zinc 5 mM 12 hrs, larvae L3		35
Zinc 4.5 mM 48 hrs, 4-day adult		48
Ethanol 2.5% 3 hrs, larvae L3		26
Ethanol 5% 3 hrs, larvae L3		26
Ethanol 10% 3 hrs, larvae L3		28
Caffeine 1.5 mg/ml 4 hrs, larvae L3		30
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		39
Caffeine 25 mg/ml 48 hrs, 4-day adult		28
Paraquat 5 mM 48 hrs, 4-day adult		35
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		25
Rotenone 8 μg 12 hrs, larvae L3		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

External Data & Images

Linkouts

BDGP expression data

•

LD13640

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0000273

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG4379-RB;CG4379-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal

lethal, with Scer\GAL4^71B

Pka-C1^{Scer\UAS.T:Zzzz\FLAG}

lethal, with Scer\GAL4^JW1

Pka-C1^{Scer\UAS.T:Zzzz\FLAG}

lethal, with Scer\GAL4^KO5

lethal, with Scer\GAL4^PP3

lethal (with Pka-C1^Cos-1)

lethal (with Pka-C1^Cos-2)

lethal (with Pka-C1^Cos-3)

lethal (with Pka-C1^Cos-4)

lethal (with Pka-C1^Cos-8)

lethal (with Pka-C1^Cos-9)

lethal | cold sensitive

Pka-C1^T4

lethal | embryonic stage | germline clone | recessive

Pka-C1^H2

lethal | larval stage | recessive

Pka-C1^B10

lethal | partially, with Scer\GAL4^e22c

Pka-C1^Scer\UAS.cKa

lethal | partially, with Scer\GAL4^RK5

lethal | partially, with Scer\GAL4^RZ4

Pka-C1^{Scer\UAS.T:Zzzz\FLAG}

lethal | pupal stage, with Scer\GAL4^JW1

Pka-C1^{K75A.Scer\UAS}

partially lethal - majority die | recessive

Pka-C1⁰⁶³⁵³

viable

viable, with Scer\GAL4^30A

Pka-C1^{K75A.Scer\UAS}

viable, with Scer\GAL4^JW1

Pka-C1^{W224R.Scer\UAS.T:Zzzz\FLAG}

viable, with Scer\GAL4^KO5

viable, with Scer\GAL4^pnr-MD237

Pka-C1^GD1276

viable, with Scer\GAL4^PP3

Pka-C1^{W224R.Scer\UAS.T:Zzzz\FLAG}

viable, with Scer\GAL4^RK5

Pka-C1^{K75A.Scer\UAS}

Sterility

Allele

female sterile

fertile, with Scer\GAL4^30A

Pka-C1^{K75A.Scer\UAS}

Other Phenotypes

Allele

behavior defective

chemical sensitive | dominant

circadian rhythm defective

circadian rhythm defective | cold sensitive

Pka-C1^X4

decreased cell death | somatic clone

Pka-C1^E95

developmental rate defective, with Scer\GAL4^30A

Pka-C1^Scer\UAS.cKa

locomotor rhythm defective

Pka-C1^T4

memory defective

Pka-C1^hs.PD

memory defective, with Scer\GAL4^c309

Pka-C1^{Scer\UAS.T:Zzzz\FLAG}

memory defective | dominant

neuroanatomy defective

Pka-C1^B3

neurophysiology defective

Pka-C1^X4

neurophysiology defective | recessive

Pka-C1^X4

planar polarity defective | somatic clone

Pka-C1⁰¹²⁷²

tumorigenic, with Scer\GAL4^30A

Pka-C1^Scer\UAS.cKa

visible

visible, with Scer\GAL4^30A

visible, with Scer\GAL4^pnr-MD237

Pka-C1^GD1276

visible, with Scer\GAL4^RK5

visible, with Scer\GAL4^RZ4

visible | cell non-autonomous | recessive | somatic clone

Pka-C1^E95

visible | cell non-autonomous | somatic clone

Pka-C1^K2

visible | recessive

Pka-C1^k11302

visible | recessive | somatic clone

Pka-C1^E95

visible | somatic clone

Pka-C1^unspecified

wild-type

wild-type, with Scer\GAL4^RK5

Pka-C1^{K75A.Scer\UAS}

Phenotype manifest in

Allele

adult, with Scer\GAL4^RK5

adult abdomen & cuticle | anterior compartment

Pka-C1^E95

adult abdomen & macrochaeta | anterior compartment

Pka-C1^E95

adult abdomen & microchaeta | anterior compartment

Pka-C1^E95

adult thorax, with Scer\GAL4^JW1

Pka-C1^{K75A.Scer\UAS}

anal plate

Pka-C1^B3

antenna

Pka-C1^H2

border follicle cell

border follicle cell | ectopic | somatic clone

Pka-C1^H2

clasper

Pka-C1^B3

egg

Pka-C1⁰¹²⁷²

egg chamber | germline clone

epiproct

Pka-C1^B3

eye

eye, with Scer\GAL4^GMR.PF

Pka-C1^Scer\UAS.cKa

eye, with Scer\GAL4^RK5

eye | cell non-autonomous | somatic clone

Pka-C1^K2

eye-antennal disc

Pka-C1^k08918

eye disc | somatic clone

Pka-C1⁰¹²⁷²

eye equator | ectopic

Pka-C1⁰¹²⁷²

eye equator | ectopic | somatic clone

Pka-C1⁰¹²⁷²

eye photoreceptor cell

Pka-C1^E95

female gonopod

Pka-C1^B3

follicle cell | ectopic | somatic clone

Pka-C1^H2

genital arch

Pka-C1^B3

germline cell | embryonic stage | germline clone

Pka-C1^H2

gonopod thorn bristle

Pka-C1^B3

haltere

hypoproct

Pka-C1^B3

lateral plate

Pka-C1^B3

leg

leg | anterior compartment

leg | ectopic | somatic clone

Pka-C1^E95

leg | somatic clone

macrochaeta, with Scer\GAL4^RK5

macrochaeta | cell non-autonomous | somatic clone

Pka-C1^E95

macrochaeta | somatic clone

Pka-C1^E95

melanotic mass, with Scer\GAL4^30A

Pka-C1^Scer\UAS.cKa

mesothoracic tergum, with Scer\GAL4^pnr-MD237

Pka-C1^GD1276

mesothoracic tergum & macrochaeta | somatic clone | cell non-autonomous

Pka-C1^E95

mesothoracic tergum | ectopic, with Scer\GAL4^JW1

Pka-C1^{K75A.Scer\UAS}

microtubule organizing center | germline clone

Pka-C1^H2

morphogenetic furrow

Pka-C1⁰¹²⁷²

neuromuscular junction

neuron

Pka-C1^X4

nurse cell

nurse cell, with Scer\GAL4^nos.PG

Pka-C1^Scer\UAS.cKa

nurse cell | germline clone

ommatidium

Pka-C1^E95

ommatidium | somatic clone

Pka-C1⁰¹²⁷²

oocyte

oocyte & microtubule | germ-line clone

Pka-C1^H2

oocyte | germline clone

Pka-C1^H2

penis

Pka-C1^B3

polar follicle cell | ectopic | somatic clone

Pka-C1^H2

scutum

scutum | somatic clone

Pka-C1^E95

sex comb | ectopic | somatic clone

Pka-C1^H2

spermathecum

Pka-C1^B3

wing

wing, with Scer\GAL4^30A

wing, with Scer\GAL4^71B

Pka-C1^Scer\UAS.cKa

wing, with Scer\GAL4⁶⁸⁴

Pka-C1^Scer\UAS.cKa

wing, with Scer\GAL4^JW1

Pka-C1^{K75A.Scer\UAS}

wing, with Scer\GAL4^KO5

Pka-C1^Scer\UAS.cKa

wing, with Scer\GAL4^PP3

Pka-C1^{K75A.Scer\UAS}

wing, with Scer\GAL4^RK5

wing, with Scer\GAL4^RZ4

Pka-C1^{Scer\UAS.T:Zzzz\FLAG}

wing | anterior, with Scer\GAL4^KO5

Pka-C1^{K75A.Scer\UAS}

wing | anterior, with Scer\GAL4^PP3

Pka-C1^{K75A.Scer\UAS}

wing | anterior, with Scer\GAL4^RZ4

Pka-C1^{K75A.Scer\UAS}

wing | anterior compartment

wing | anterior compartment | somatic clone

wing | ectopic | somatic clone

Pka-C1^E95

wing | somatic clone

wing disc

Pka-C1^unspecified

wing margin bristle, with Scer\GAL4^RZ4

Pka-C1^{Scer\UAS.T:Zzzz\FLAG}

wing vein | ectopic, with Scer\GAL4^71B

Pka-C1^Scer\UAS.cKa

wing vein | ectopic, with Scer\GAL4⁶⁸⁴

Pka-C1^Scer\UAS.cKa

wing vein | ectopic, with Scer\GAL4^RZ4

Pka-C1^{K75A.Scer\UAS}

wing vein L3 | ectopic | somatic clone

Pka-C1^unspecified

Classical Alleles ( 74 )

For All Classical Alleles Show

Allele of Pka-C1	Class	Mutagen	Stocks	Known lesion
Pka-C1^BG02142		P-element activity	3	--
Pka-C1^H2	amorphic allele - genetic evidence, loss of function allele		2	Yes
Pka-C1^DN	antimorphic allele - genetic evidence		2	--
Pka-C1^76a3		ethyl nitrosourea	1	--
Pka-C1^B10	hypomorphic allele - genetic evidence	ethyl methanesulfonate	1	Yes
Pka-C1^CB-0733-3			1	--
Pka-C1^e02391		piggyBac transposase	1	--
Pka-C1^f00909		piggyBac transposase	1	--
Pka-C1^f00921		piggyBac transposase	1	--
Pka-C1^f06704		piggyBac transposase	1	--
Pka-C1^k00804		P-element activity	1	--
Pka-C1^MI07503			1	--
Pka-C1^B3	amorphic allele - genetic evidence, loss of function allele		0	Yes
Pka-C1^E95	loss of function allele, amorphic allele - genetic evidence	ethyl methanesulfonate	0	--
Pka-C1⁰¹²⁴²		P-element activity	0	--
Pka-C1⁰¹²⁷²		P-element activity	0	Yes
Pka-C1⁰⁶³⁵³	hypomorphic allele - genetic evidence	P-element activity	0	Yes
Pka-C1⁰⁷¹⁶²		P-element activity	0	--
Pka-C1⁶¹		P-element activity	0	Yes
Pka-C1^A13	hypomorphic allele - genetic evidence		0	Yes
Pka-C1^B12			0	Yes
Pka-C1^C2	hypomorphic allele - genetic evidence		0	Yes
Pka-C1^C4			0	Yes
Pka-C1^CB03724		P-element activity	0	Yes
Pka-C1^Cos-1		ethyl methanesulfonate	0	--
Pka-C1^Cos-2	neomorphic allele - genetic evidence	ethyl methanesulfonate	0	Yes
Pka-C1^Cos-2R+1		X ray	0	--
Pka-C1^Cos-3	neomorphic allele - genetic evidence	X ray ethyl methanesulfonate	0	Yes
Pka-C1^Cos-3R+2		X ray	0	--
Pka-C1^Cos-3R+4		X ray	0	--
Pka-C1^Cos-3R+E10		ethyl methanesulfonate	0	--
Pka-C1^Cos-3R+E11		ethyl methanesulfonate	0	--
Pka-C1^Cos-3R+E12		ethyl methanesulfonate	0	--
Pka-C1^Cos-3R+E13		ethyl methanesulfonate	0	--
Pka-C1^Cos-3R+E14		ethyl methanesulfonate	0	--
Pka-C1^Cos-3R+E15		ethyl methanesulfonate	0	--
Pka-C1^Cos-3R+E16		ethyl methanesulfonate	0	--
Pka-C1^Cos-3R+E18		ethyl methanesulfonate	0	--
Pka-C1^Cos-3R+E19		ethyl methanesulfonate	0	--
Pka-C1^Cos-3R+E1		ethyl methanesulfonate	0	--
Pka-C1^Cos-3R+E20		ethyl methanesulfonate	0	--
Pka-C1^Cos-3R+E2		ethyl methanesulfonate	0	--
Pka-C1^Cos-3R+E3		ethyl methanesulfonate	0	--
Pka-C1^Cos-3R+E4		ethyl methanesulfonate	0	--
Pka-C1^Cos-3R+E5		ethyl methanesulfonate	0	--
Pka-C1^Cos-3R+E6		ethyl methanesulfonate	0	--
Pka-C1^Cos-3R+E9		ethyl methanesulfonate	0	--
Pka-C1^Cos-4		ethyl methanesulfonate	0	--
Pka-C1^Cos-5		X ray	0	--
Pka-C1^Cos-6		X ray	0	--
Pka-C1^Cos-7		X ray	0	--
Pka-C1^Cos-8	neomorphic allele - genetic evidence	ethyl methanesulfonate	0	Yes
Pka-C1^Cos-9	neomorphic allele - genetic evidence	ethyl methanesulfonate	0	Yes
Pka-C1^D30			0	Yes
Pka-C1^G9			0	Yes
Pka-C1^GS12852			0	--
Pka-C1^H3	loss of function allele		0	Yes
Pka-C1^k00228		P-element activity	0	--
Pka-C1^k06108		P-element activity	0	--
Pka-C1^k06118		P-element activity	0	--
Pka-C1^k08008		P-element activity	0	--
Pka-C1^k08918	hypomorphic allele - genetic evidence	P-element activity	0	Yes
Pka-C1^k11302		P-element activity	0	Yes
Pka-C1^k16718		P-element activity	0	--
Pka-C1^K2			0	--
Pka-C1^MB581	hypomorphic allele - genetic evidence	P-element activity	0	Yes
Pka-C1^MntA.M			0	Yes
Pka-C1^rM692		P-element activity	0	--
Pka-C1^rN167		P-element activity	0	--
Pka-C1^s4402		P-element activity	0	--
Pka-C1^T4			0	--
Pka-C1^Tw2	hypomorphic allele - genetic evidence		0	Yes
Pka-C1^unspecified			0	--
Pka-C1^X4			0	--

Alleles Carried on Transgenic Constructs ( 16 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Pka-C1	Mutagen	Stocks	Known lesion
Pka-C1^{K75A.Scer\UAS}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	3	Yes
Pka-C1^{Scer\UAS.T:Zzzz\FLAG}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	3	Yes
Pka-C1^GL00038	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Pka-C1^JF01188	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Pka-C1^JF01218	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Pka-C1^KK108966	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Pka-C1^{W224R.Scer\UAS.T:Zzzz\FLAG}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	1	Yes
Pka-C1^+mTgDC0	in vitro construct - minigene	0	Yes
Pka-C1^Act5C.PC	in vitro construct - regulatory fusion	0	Yes
Pka-C1^Act5C.PN	in vitro construct - regulatory fusion in vitro construct - minigene	0	Yes
Pka-C1^{Act5C.T:Ivir\HA1}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
Pka-C1^dsRNA.cLa	in vitro construct - RNAi	0	Yes
Pka-C1^GD1276	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
Pka-C1^hs.PD	in vitro construct - regulatory fusion	0	Yes
Pka-C1^Scer\UAS.cKa	in vitro construct - regulatory fusion	0	Yes
Pka-C1^{W224R.Scer\UAS}	in vitro construct - regulatory fusion in vitro construct - amino acid replacement	0	Yes

Aneuploid Aberrations

Disrupted in

Df(2L)30A-C

(Spradling et al., 1999, Lane and Kalderon, 1993, Skoulakis et al., 1993, BDGP Project Members, 1994-1999)

Df(2L)232

(Bantignies et al., 2000, BDGP Project Members, 1994-1999)

Df(2L)Cos-3R+3

(Grau and Simpson, 1987)

Df(2L)Cos-3R+5

(Grau and Simpson, 1987)

Df(2L)Cos-3R+6

(Grau and Simpson, 1987)

Df(2L)Exel6024

(Ryder, 2004.4.26)

Df(2L)s1402

Df(2L)Tw2

(Lane and Kalderon, 1993, Majercak et al., 1997)

Df(2L)γ7

(Lane and Kalderon, 1993, Skoulakis et al., 1993, Majercak et al., 1997)

Df(2L)γ15

Not disrupted in

Df(2L)132

Df(2L)166

Df(2L)169

Df(2L)176

Df(2L)200

Df(2L)210

Df(2L)255

Df(2L)A4

(BDGP Project Members, 1994-1999)

Df(2L)ED695

(Levine et al., 2007)

Df(2L)J2

Df(2L)J27

(BDGP Project Members, 1994-1999)

Df(2L)TE2

Df(2L)TE30Cb-1

(Levine et al., 2007)

Df(2L)γ1

Df(2L)γ4

Df(2L)γ5

Df(2L)γ12

Df(2L)γ25

Df(2L)γ27

Df(2L)γ29

P{Act5C(FRT.y⁺CD2)PKA.A902}

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{UAS-Pka-C1.W224R.F}

P{UAS-Pka-C1.W224R}

heat-shock construct

P{CAT(1-353)}

characterization construct

P{Act5C(-FRT)PKA.A902}

P{TgDC0}

P{UAS-PKA.mC.FRT}

P{UAS-PKA.mC}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

Mi{MIC}Pka-C1^MI07503

P{GT1}Pka-C1^BG02142

P{PTT-GB}Pka-C1^CB03724

P{RS3}Pka-C1^CB-0733-3

P{Switch2}GSG329-1

PBac{RB}Pka-C1^e02391

PBac{WH}Pka-C1^f00909

PBac{WH}Pka-C1^f00921

PBac{WH}Pka-C1^f06704

insertion of enhancer trap

P{lacW}l(2)SH0310^SH0310

P{PZ}Pka-C1⁰¹²⁴²

P{PZ}Pka-C1⁰¹²⁷²

P{PZ}Pka-C1⁰⁶³⁵³

P{PZ}Pka-C1⁰⁷¹⁶²

P{PZ}Pka-C1^rM692

P{PZ}Pka-C1^rN167

insertion of mobile activating element

P{GSV6}hoip^GS12852

Gene Ontology: Function, Process & Cellular Component ( 29 unique terms )

Terms Based on Experimental Evidence ( 17 terms )

Molecular Function

CV term

(Guruharsha et al., 2011)

References

protein binding

inferred from physical interaction with Dere\Pka-R2

inferred from physical interaction with slo

(Wang et al., 1999)

Biological Process

CV term

anesthesia-resistant memory

References

inferred from mutant phenotype

(Horiuchi et al., 2008)

anterior/posterior pattern specification, imaginal disc

inferred from mutant phenotype

(Pan and Rubin, 1995)

imaginal disc-derived wing morphogenesis

inferred from mutant phenotype

(Pan and Rubin, 1995)

learning or memory

inferred from mutant phenotype

negative regulation of smoothened signaling pathway

inferred from genetic interaction with Su(fu)

(Ohlmeyer and Kalderon, 1998)

oocyte anterior/posterior axis specification

inferred from mutant phenotype

(Lane and Kalderon, 1994, Steinhauer and Kalderon, 2005)

oocyte microtubule cytoskeleton polarization

inferred from mutant phenotype

(Lane and Kalderon, 1994)

oogenesis

inferred from mutant phenotype

positive regulation of hh target transcription factor activity

inferred from mutant phenotype

(Wang and Holmgren, 2000)

protein phosphorylation

inferred from mutant phenotype

(Iijima-Ando et al., 2009)

regulation of bicoid mRNA localization

inferred from mutant phenotype

(Lane and Kalderon, 1994)

regulation of circadian sleep/wake cycle, sleep

inferred from mutant phenotype

(Yuan et al., 2006)

regulation of pole plasm oskar mRNA localization

inferred from mutant phenotype

(Lane and Kalderon, 1994)

regulation of synaptic transmission

inferred from mutant phenotype

(Yao and Wu, 2001)

rhythmic behavior

inferred from mutant phenotype

(Levine et al., 1994)

Cellular Component

CV term

(Lane and Kalderon, 1995, Khanna et al., 2010)

References

plasma membrane

inferred from direct assay

Terms Based on Predictions or Assertions ( 16 terms )

Molecular Function

CV term

(FlyBase Curators et al., 2004-)

References

ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR000961, InterPro:IPR002290, InterPro:IPR017441

cAMP-dependent protein kinase activity

inferred from sequence or structural similarity

(Swiss-Prot Project Members, 1989.10.1)

non-traceable author statement

protein serine/threonine kinase activity

inferred from electronic annotation with InterPro:IPR000961, InterPro:IPR008271

(FlyBase Curators et al., 2004-)

non-traceable author statement

(Morrison et al., 2000)

Biological Process

CV term

behavioral response to ethanol

References

non-traceable author statement

(Rothenfluh and Heberlein, 2002)

traceable author statement

cAMP-mediated signaling

non-traceable author statement

(Roman and Davis, 2001, Burrell and Sahley, 2001, Korzus, 2003)

traceable author statement

(Cooperstock and Lipshitz, 1997)

compound eye development

non-traceable author statement

imaginal disc-derived leg morphogenesis

non-traceable author statement

(Burrell and Sahley, 2001)

learning

non-traceable author statement

learning or memory

non-traceable author statement

(Korzus, 2003, Sokolowski, 2001)

locomotor rhythm

non-traceable author statement

(Burrell and Sahley, 2001)

memory

non-traceable author statement

olfactory learning

non-traceable author statement

(Roman and Davis, 2001)

oogenesis

traceable author statement

(Cooperstock and Lipshitz, 1997)

protein phosphorylation

inferred from sequence or structural similarity

(Morrison et al., 2000, Swiss-Prot Project Members, 1989.10.1)

non-traceable author statement

rhythmic behavior

traceable author statement

(Stanewsky, 2002, Stanewsky, 2003)

Cellular Component

CV term

cAMP-dependent protein kinase complex

References

inferred from sequence or structural similarity