The gene Centrosomal protein 190kD is referred to in FlyBase by the symbol Dmel\Cp190 (CG6384, FBgn0000283). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: chromatin binding; microtubule binding; protein binding; DNA binding; chromatin insulator sequence binding. There is experimental evidence that it is involved in the biological process: nuclear axial expansion. 37 alleles are reported. The phenotypes of these alleles are annotated with: centrosome; microtubule; cleavage nucleus. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: BTB/POZ; BTB/POZ fold; BTB/POZ-like; Zinc finger, C2H2; Zinc finger, C2H2-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval salivary gland, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult thoracico-abdominal ganglion, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, larval trachea, adult spermathecae, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1631940_s_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of Cp190. Gene sequence location is 3R:11096154..11100963.
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Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval salivary gland, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult thoracico-abdominal ganglion, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, larval trachea, adult spermathecae, adult male accessory gland, larval/adult carcass.
[download data (TSV)]
Guide to FlyAtlas expression level colors
No expression (0 - 9.999)
Low expression (10 - 99.999)
Moderate expression (100 - 499.999)
High level expression (500 - 999.999)
Very high expression (>999.999)
Linear, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
803.275
Larval Midgut
185.9
Larval Hindgut
234.1
Larval Malpighian Tubules
227.8
Larval Fat Body
225.2
Larval Salivary Gland
573.6
Larval Trachea
494.575
Larval Carcass
284.175
Adult Head
255.1
Adult Eye
276.125
Adult Brain
558.1
Adult Thoracic-Abdominal Ganglion
327
Adult Crop
295.8
Adult Midgut
215
Adult Hindgut
230.5
Adult Malpighian Tubules
382.8
Adult Fat Body
193.9
Adult Salivary Gland
366
Adult Heart
236.475
Adult VirginFemale Spermatheca
226.7
Adult InseminatedFemale Spermatheca
246.4
Adult Ovary
999.1
Adult Testis
80.8
Adult Male Accessory Gland
231
Adult Carcass
204.4
Expression Level Scale
None
Low
Moderate
High
Very high
Linear, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
(803.275)
Larval Midgut
185.9
Larval Hindgut
234.1
Larval Malpighian Tubules
227.8
Larval Fat Body
225.2
Larval Salivary Gland
(573.6)
Larval Trachea
494.575
Larval Carcass
284.175
Adult Head
255.1
Adult Eye
276.125
Adult Brain
(558.1)
Adult Thoracic-Abdominal Ganglion
327
Adult Crop
295.8
Adult Midgut
215
Adult Hindgut
230.5
Adult Malpighian Tubules
382.8
Adult Fat Body
193.9
Adult Salivary Gland
366
Adult Heart
236.475
Adult VirginFemale Spermatheca
226.7
Adult InseminatedFemale Spermatheca
246.4
Adult Ovary
(999.1)
Adult Testis
80.8
Adult Male Accessory Gland
231
Adult Carcass
204.4
Expression Level Scale
None
Low
Moderate
High
Linear, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
803.275
Larval Midgut
185.9
Larval Hindgut
234.1
Larval Malpighian Tubules
227.8
Larval Fat Body
225.2
Larval Salivary Gland
573.6
Larval Trachea
494.575
Larval Carcass
284.175
Adult Head
255.1
Adult Eye
276.125
Adult Brain
558.1
Adult Thoracic-Abdominal Ganglion
327
Adult Crop
295.8
Adult Midgut
215
Adult Hindgut
230.5
Adult Malpighian Tubules
382.8
Adult Fat Body
193.9
Adult Salivary Gland
366
Adult Heart
236.475
Adult VirginFemale Spermatheca
226.7
Adult InseminatedFemale Spermatheca
246.4
Adult Ovary
999.1
Adult Testis
80.8
Adult Male Accessory Gland
231
Adult Carcass
204.4
Expression Level Scale
None
Low
Moderate
High
Very high
Linear, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
803.275
Larval Midgut
185.9
Larval Hindgut
234.1
Larval Malpighian Tubules
227.8
Larval Fat Body
225.2
Larval Salivary Gland
573.6
Larval Trachea
494.575
Larval Carcass
284.175
Adult Head
255.1
Adult Eye
276.125
Adult Brain
558.1
Adult Thoracic-Abdominal Ganglion
327
Adult Crop
295.8
Adult Midgut
215
Adult Hindgut
230.5
Adult Malpighian Tubules
382.8
Adult Fat Body
193.9
Adult Salivary Gland
366
Adult Heart
236.475
Adult VirginFemale Spermatheca
226.7
Adult InseminatedFemale Spermatheca
246.4
Adult Ovary
999.1
Adult Testis
80.8
Adult Male Accessory Gland
231
Adult Carcass
204.4
Expression Level Scale
Very high
log, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
803.275
Larval Midgut
185.9
Larval Hindgut
234.1
Larval Malpighian Tubules
227.8
Larval Fat Body
225.2
Larval Salivary Gland
573.6
Larval Trachea
494.575
Larval Carcass
284.175
Adult Head
255.1
Adult Eye
276.125
Adult Brain
558.1
Adult Thoracic-Abdominal Ganglion
327
Adult Crop
295.8
Adult Midgut
215
Adult Hindgut
230.5
Adult Malpighian Tubules
382.8
Adult Fat Body
193.9
Adult Salivary Gland
366
Adult Heart
236.475
Adult VirginFemale Spermatheca
226.7
Adult InseminatedFemale Spermatheca
246.4
Adult Ovary
999.1
Adult Testis
80.8
Adult Male Accessory Gland
231
Adult Carcass
204.4
Expression Level Scale
None
Low
Moderate
High
Very high
log, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
803.275
Larval Midgut
185.9
Larval Hindgut
234.1
Larval Malpighian Tubules
227.8
Larval Fat Body
225.2
Larval Salivary Gland
573.6
Larval Trachea
494.575
Larval Carcass
284.175
Adult Head
255.1
Adult Eye
276.125
Adult Brain
558.1
Adult Thoracic-Abdominal Ganglion
327
Adult Crop
295.8
Adult Midgut
215
Adult Hindgut
230.5
Adult Malpighian Tubules
382.8
Adult Fat Body
193.9
Adult Salivary Gland
366
Adult Heart
236.475
Adult VirginFemale Spermatheca
226.7
Adult InseminatedFemale Spermatheca
246.4
Adult Ovary
(999.1)
Adult Testis
80.8
Adult Male Accessory Gland
231
Adult Carcass
204.4
Expression Level Scale
None
Low
Moderate
High
log, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
803.275
Larval Midgut
185.9
Larval Hindgut
234.1
Larval Malpighian Tubules
227.8
Larval Fat Body
225.2
Larval Salivary Gland
573.6
Larval Trachea
494.575
Larval Carcass
284.175
Adult Head
255.1
Adult Eye
276.125
Adult Brain
558.1
Adult Thoracic-Abdominal Ganglion
327
Adult Crop
295.8
Adult Midgut
215
Adult Hindgut
230.5
Adult Malpighian Tubules
382.8
Adult Fat Body
193.9
Adult Salivary Gland
366
Adult Heart
236.475
Adult VirginFemale Spermatheca
226.7
Adult InseminatedFemale Spermatheca
246.4
Adult Ovary
999.1
Adult Testis
80.8
Adult Male Accessory Gland
231
Adult Carcass
204.4
Expression Level Scale
None
Low
Moderate
High
Very high
log, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
803.275
Larval Midgut
185.9
Larval Hindgut
234.1
Larval Malpighian Tubules
227.8
Larval Fat Body
225.2
Larval Salivary Gland
573.6
Larval Trachea
494.575
Larval Carcass
284.175
Adult Head
255.1
Adult Eye
276.125
Adult Brain
558.1
Adult Thoracic-Abdominal Ganglion
327
Adult Crop
295.8
Adult Midgut
215
Adult Hindgut
230.5
Adult Malpighian Tubules
382.8
Adult Fat Body
193.9
Adult Salivary Gland
366
Adult Heart
236.475
Adult VirginFemale Spermatheca
226.7
Adult InseminatedFemale Spermatheca
246.4
Adult Ovary
999.1
Adult Testis
80.8
Adult Male Accessory Gland
231
Adult Carcass
204.4
Expression Level Scale
None
Low
Moderate
High
Very high
Heatmap
Tissue
Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass
FlyAtlas Organ/Tissue Expression, larval vs. adult
Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages.
[download data (TSV)]
Please Note FlyBase no
longer curates genomic clone accessions so this list
may not be complete
cDNA Clones ( 98 )
Please Note
This section lists
cDNAs and ESTs that fall within the genomic extent
of the gene model, which may include cDNAs and ESTs
of genes within introns, or of overlapping genes.
Please see GBrowse for alignment of the cDNAs and ESTs
to the gene model.
DNA-protein interactions: genome-wide binding profile assayed for Cp190 protein in 0-12 hr embryos; see Insulator_Class_I.mE01 collection report. Individual protein-binding experiments listed under "Samples" at GEO: 16245 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE16245).
The introduction of dsRNA made from templates generated with primers directed against this gene into S2 cells prevents the accumulation of Map60 to mitotic centrosomes.
Cp190 requires su(Hw) in order to bind to the insulator present in the gypsy retrotransposon, but su(Hw) is not necessary for the interaction of Cp190 with endogenous insulator sites.
Fluorescently labelled bacterially expressed Cp190 is injected into living embryos and protein behaviour during the rapid syncytial blastoderm divisions (nuclear cycles 10-13) is followed.
The region between amino acids 385 and 508 of the Cp190 protein is sufficient for good centrosomal localisation. A nuclear localisation domain is located between amino acids 201 and 271.
Cp190 is associated with the centrosome during mitosis and during interphase the protein is associated with a large number of specific chromosomal sites. Cp190 binds to chromatin in association with another centrosomal protein, Map60.
By using monoclonal antibodies that recognize protein components of the mitotic apparatus to screen expression libraries, Cen185, which encodes a protein associated with the centrosome, was cloned.
Lim et al., 2013, Nucleic Acids Res. 41(5): 2963--2980
Genome-wide localization of exosome components to active promoters and chromatin insulators in Drosophila. [FBrf0221054]
Antao et al., 2012, Mol. Cell. Biol. 32(12): 2170--2182
Protein Landscape at Drosophila melanogaster Telomere-Associated Sequence Repeats. [FBrf0218429]
Crawford et al., 2012, Cell Death Differ. 19(12): 2040--2048
Conservation of caspase substrates across metazoans suggests hierarchical importance of signaling pathways over specific targets and cleavage site motifs in apoptosis. [FBrf0220233]
Fukuyama et al., 2012, J. Proteomics 75(15): 4610--4619
On-bead tryptic proteolysis: An attractive procedure for LC-MS/MS analysis of the Drosophila caspase 8 protein complex during immune response against bacteria. [FBrf0218992]
Golovnin et al., 2012, J. Cell Sci. 125(8): 2064--2074
SUMO conjugation is required for the assembly of Drosophila Su(Hw) and Mod(mdg4) into insulator bodies that facilitate insulator complex formation. [FBrf0218398]
Gopalakrishnan et al., 2012, Nat. Cell Biol. 14(8): 865--873
Tubulin nucleotide status controls Sas-4-dependent pericentriolar material recruitment. [FBrf0219801]
Gurudatta et al., 2012, Dev. Biol. 369(1): 124--132
The BEAF insulator regulates genes involved in cell polarity and neoplastic growth. [FBrf0219796]
Maeng et al., 2012, Mol. Cells 33(5): 457--463
The BTB/POZ-ZF transcription factor dPLZF is involved in Ras/ERK signaling during Drosophila wing development. [FBrf0218378]
Matzat et al., 2012, PLoS Genet. 8(11): e1003069
Tissue-specific regulation of chromatin insulator function. [FBrf0220170]
Philip et al., 2012, BMC Genomics 13(1): 97
Sequence signatures involved in targeting the male-specific lethal complex to X-chromosomal genes in Drosophila melanogaster. [FBrf0218340]
Riddle et al., 2012, PLoS Genet. 8(9): e1002954
Enrichment of HP1a on Drosophila Chromosome 4 Genes Creates an Alternate Chromatin Structure Critical for Regulation in this Heterochromatic Domain. [FBrf0219520]
Sakoparnig et al., 2012, J. Comput. Biol. 19(2): 126--138
Binding profiles of chromatin-modifying proteins are predictive for transcriptional activity and promoter-proximal pausing. [FBrf0217402]
Schwartz et al., 2012, Genome Res. 22(11): 2188--2198
Nature and function of insulator protein binding sites in the Drosophila genome. [FBrf0219925]
Soshnev et al., 2012, Nucleic Acids Res. 40(12): 5415--5431
Genome-wide studies of the multi-zinc finger Drosophila Suppressor of Hairy-wing protein in the ovary. [FBrf0218724]
Baxley et al., 2011, Dev. Biol. 356(2): 398--410
The role of the Suppressor of Hairy-wing insulator protein in Drosophila oogenesis. [FBrf0214454]
Bonchuk et al., 2011, J. Mol. Biol. 412(3): 423--436
Drosophila BTB/POZ Domains of "ttk Group" Can Form Multimers and Selectively Interact with Each Other. [FBrf0215054]
Demakov et al., 2011, BMC Genomics 12: 566
Protein composition of interband regions in polytene and cell line chromosomes of Drosophila melanogaster. [FBrf0216974]
Erokhin et al., 2011, Development 138(18): 4097--4106
Insulators form gene loops by interacting with promoters in Drosophila. [FBrf0214806]
Gopalakrishnan et al., 2011, Nat. Commun. 2: 359
Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome. [FBrf0216370]
Ma and Jarman, 2011, J. Cell Sci. 124(15): 2622--2630
Dilatory is a Drosophila protein related to AZI1 (CEP131) that is located at the ciliary base and required for cilium formation. [FBrf0214370]
Moshkovich et al., 2011, Genes Dev. 25(16): 1686--1701
RNAi-independent role for Argonaute2 in CTCF/CP190 chromatin insulator function. [FBrf0214686]
Nègre et al., 2011, Nature 471(7339): 527--531
A cis-regulatory map of the Drosophila genome. [FBrf0213303]
Rach et al., 2011, PLoS Genet. 7(1): e1001274
Transcription initiation patterns indicate divergent strategies for gene regulation at the chromatin level. [FBrf0214353]
Ramos et al., 2011, PLoS ONE 6(1): e16562
DNA Topoisomerase II Modulates Insulator Function in Drosophila. [FBrf0213027]
Richter et al., 2011, Nat. Cell Biol. 13(9): 1029--1039
The tumour suppressor L(3)mbt inhibits neuroepithelial proliferation and acts on insulator elements. [FBrf0215050]
Sultana et al., 2011, Nucleic Acids Res. 39(9): 3543--3557
A BEAF dependent chromatin domain boundary separates myoglianin and eyeless genes of Drosophila melanogaster. [FBrf0213641]
Toku et al., 2011, Comput. Biol. Chem. 35(5): 282--292
Reconstruction and crosstalk of protein-protein interaction networks of Wnt and Hedgehog signaling in Drosophila melanogaster. [FBrf0216393]
Vatolina et al., 2011, Genetika, Moscow 47(5): 597--609
[Identification and molecular genetic characterization of the polytene chromosome interbands in Drosophila melanogaster]. [FBrf0214456]
Vatolina et al., 2011, Russ. J. Genet. 47(5): 521--532
Identification and molecular genetic characterization of the polytene chromosome interbands in Drosophila melanogaster. [FBrf0216692]
Wood et al., 2011, Mol. Cell 44(1): 29--38
Regulation of chromatin organization and inducible gene expression by a Drosophila insulator. [FBrf0216321]