• Home
• Tools
  • BLAST
  • GBrowse
  • QueryBuilder
  • TermLink
  • ImageBrowse
  • Interactions Browser
  • Apollo
  • CytoSearch
  • QuickSearch
  • Batch Download
  • Coordinate Converter
  • Google™ FlyBase
  • Find A Person
  • Update An Address
  • Add A New Person
• Files
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases FTP
  • Genomes FTP
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Synteny Table
  • Drosophilidae
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All resources
  • Interactive Fly
  • Textpresso for Fly
  • AAA site
  • BDGP
  • DHGP
  • DGRC
  • Model Organisms
  • Stock Collections:
  • Bloomington
  • DrosDel
  • Ehime
  • Exelixis (Harvard)
  • GDP (Baylor)
  • Kyoto
  • NIG-Fly
  • Szeged
  • Tucson
  • VDRC
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Known Problems
  • Contact FlyBase
• Archives
  • Prior Home Page
  • R4.3 BLAST
  • R4.3 GBrowse
  • Older Archives

Gene Dmel\Cp190

General Information
Symbol	Dmel\Cp190	Species	D. melanogaster
Name	Centrosomal protein 190kD	Annotation symbol	CG6384
Feature type	protein_coding_gene	FlyBase ID	FBgn0000283
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	88E9-88E11	Sequence location	3R:11,096,146..11,100,927 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Centrosomal protein 190kD is referred to in FlyBase by the symbol Cp190 (CG6384, FBgn0000283). It has the cytological map location 88E9-88E11. Its sequence location is 3R:11096146..11100927. Its molecular function is described as: microtubule binding; zinc ion binding. It is involved in the biological processes: microtubule-based process; nuclear axial expansion. 31 alleles are reported. The phenotypes of these alleles are annotated with: cleavage nucleus; centrosome; microtubule. It has 2 annotated transcripts and 2 annotated polypeptides.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 88E9-88E11   Limits computationally determined from genome sequence between P{lacW}Hsc70-4L3929 and P{lacW}MRG15j6A3
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Cp190 for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Cp190-RA FBtr0083126  NM_079635   4086   1096 Cp190-RB FBtr0083127  NM_169632   4184   1096
Additional Transcript Data & Comments
Reported size (kB)	4.0 (northern blot) (Whitfield et al., 1988)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Cp190-PA   FBpp0082580   121.7   1096   4.29     NP_524359   AAF55159 Cp190-PB   FBpp0082581   121.7   1096   4.29     NP_731998   AAN13643
Additional Polypeptide Data & Comments
Reported size (kD)	185 (kD) (Whitfield et al., 1988)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • BTB/POZ-like (IPR000210) Endoplasmic reticulum, targeting sequence (IPR000886) Zinc finger, C2H2-type (IPR007087) BTB/POZ (IPR013069) BTB/POZ fold (IPR011333) Zinc finger, C2H2-like (IPR015880)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201553   AA201898   AA202220   AA202620   AA263327   AA390436   AA390543   AA392344   AA439310   AA440189   AA440701   AA567674   AA695050   AA735482   AA816931   AA817460   AA942454   AA942461   AA948851   AA949342   AA950047   AA951062   AE003708 AAF55159   AE003708 AAN13643   AI456879   AI456997   AI457075   AI511687   AI511693   AI515569   AI515602   AI518148   AI531076   AI531180   AI532177   AI532674   AI533381   AI533383   AI541906   AI543869   AI546252   AI546487   AW941502   BG636326   BI164646   BI165847   BI353370   BI358341   BI367338   BI369885   BI371155   BI373641   BI485527   BI579101   BT010090 AAQ22559   CO154999   CO298517   CO318462   CO319369   DN153949   EC057343   EC065398   EC067496   EC084780   EC216218   EC218152   EC218826   EC220081   EC225743   EC227516   EC233510   EC250137   EC261119   EC261969   Z50021 CAA90324
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q24478
Maps to
Does NOT map to
Identified with	LD02352 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 9497
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  adult stage | female   (Whitfield et al., 1988)  embryonic stage | 0-16 hr   (Whitfield et al., 1988)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference  embryonic stage | blastoderm   (Whitfield et al., 1988)
Marker for
Subcellular Localization
CV Term	nucleus centrosome (Whitfield et al., 1988)
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000283 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0000283 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0000283
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Cp190CB04933 Cp190H4-1 lethal Cp190H31-2 Cp190H53-2 lethal | partially | pupal stage | recessive Cp190Ubi-p63E.PB lethal | larval stage | partially (with Df(3R)P280-NR27) Cp1901 Cp1902 lethal | larval stage | partially (with Cp1902) Cp1901 lethal | larval stage | partially (with Cp1901) Cp1902 lethal | larval stage | partially | recessive Cp1901 Cp1902 lethal | pupal stage | recessive Cp190ΔM.Ubi-p63E lethal | pharate adult stage (with Df(3R)P280-NR27) Cp1901 Cp1902 lethal | pharate adult stage (with Cp1902) Cp1901 lethal | pharate adult stage (with Cp1901) Cp1902 lethal | pharate adult stage | recessive Cp1901 Cp1902 Phenotype manifest in Allele centrosome Cp190ΔM.Ubi-p63E microtubule Cp190ΔM.Ubi-p63E cleavage nucleus Cp1902
Classical Alleles ( 7 )
For All Classical Alleles Show
Allele of Cp190 Class Mutagen Stocks Known lesion Cp1901 loss of function ethyl methanesulfonate 0 -- Cp1902 loss of function ethyl methanesulfonate 0 -- Cp190CB04933 P-element activity 0 Yes Cp190EY20997 P-element activity 1 -- Cp190H31-2 ethyl methanesulfonate 0 Yes Cp190H4-1 hypomorph ethyl methanesulfonate 0 Yes Cp190H53-2 ethyl methanesulfonate 0 Yes
Alleles Carried on Transgenic Constructs ( 24 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Cp190 Class Mutagen Stocks Known lesion Cp190167-1090.T:Zzzz\His6 in vitro construct | coding region fusion 0 Yes Cp190167-271 in vitro construct | other 0 Yes Cp190167-321 in vitro construct | other 0 Yes Cp190167-468 in vitro construct | other 0 Yes Cp190167-608 in vitro construct | other 0 Yes Cp190207-321 in vitro construct | other 0 Yes Cp190207-608 in vitro construct | other 0 Yes Cp190240-321 in vitro construct | other 0 Yes Cp190240-608 in vitro construct | other 0 Yes Cp190266-608 in vitro construct | other 0 Yes Cp190309-427 in vitro construct | other 0 Yes Cp190309-456 in vitro construct | other 0 Yes Cp190309-608 in vitro construct | other 0 Yes Cp190385-508 in vitro construct | other 0 Yes Cp190421-608 in vitro construct | other 0 Yes Cp190428-608 in vitro construct | other 0 Yes Cp190451-608 in vitro construct | other 0 Yes Cp190504-789 in vitro construct | other 0 Yes Cp190646-937 in vitro construct | other 0 Yes Cp190784-1090 in vitro construct | other 0 Yes Cp190GD11908 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes Cp190Ubi-p63E.PB in vitro construct | regulatory fusion 0 Yes Cp190Δ175 in vitro construct | other 0 Yes Cp190ΔM.Ubi-p63E in vitro construct | regulatory fusion in vitro construct | deletion 0 Yes
Aneuploid Aberrations
Useful duplication	Dp(3;3)C123.3
Disrupted in	Df(3R)E(mod)4-1P11 (Pai et al., 2004) Df(3R)E(mod)4-1P1 (Pai et al., 2004) Df(3R)Exel7323 (Ryder, 2004.4.26) Df(3R)P280-NR27 (Butcher et al., 2004)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11908} NA characterization construct P{Ubi-Cp190.D} NA P{Ubi-Cp190.ΔM} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{PTT-GB}Cp190CB04933 NA insertion of mobile activating element P{EPgy2}Cp190EY20997 NA
Related Comments
	Please look at the allele reports for the complete phenotype data Cp190 mutations act as recessive suppressors of gypsy-induced phenotypes, suggesting that Cp190 is an essential component of the gypsy insulator. (Pai et al., 2004) By using monoclonal antibodies that recognize protein components of the mitotic apparatus to screen expression libraries, Cen185, which encodes a protein associated with the centrosome, was cloned. (Whitfield et al., 1988) Cp190 is associated with the centrosome during mitosis and during interphase the protein is associated with a large number of specific chromosomal sites. Cp190 binds to chromatin in association with another centrosomal protein, Map60. (Whitfield et al., 1995) The region between amino acids 385 and 508 of the Cp190 protein is sufficient for good centrosomal localisation. A nuclear localisation domain is located between amino acids 201 and 271. (Oegema et al., 1995) Map60 (Butcher et al., 2004)
Gene Ontology: Function, Process & Cellular Component ( 8 )
Molecular Function
CV term Evidence References
microtubule binding inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) inferred from direct assay (Butcher et al., 2004)
zinc ion binding inferred from electronic annotation with InterPro:IPR007087, InterPro:IPR015880 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
microtubule-based process inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000)
nuclear axial expansion inferred from mutant phenotype (Chodagam et al., 2005)
Cellular Component
CV term Evidence References
centrosome inferred from direct assay (Whitfield et al., 1988) non-traceable author statement (Whitfield, 1995.7.6) inferred from direct assay (Prigent et al., 2005) inferred from direct assay (Butcher et al., 2004) inferred from direct assay (Chodagam et al., 2005)
endoplasmic reticulum inferred from electronic annotation with InterPro:IPR000886 (FlyBase Curators et al., 2004-)
microtubule associated complex inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000)
nucleus inferred from direct assay (Butcher et al., 2004)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Cp190 allele Gene References unspecified sqh (Chodagam et al., 2005) mod(mdg4) (Pai et al., 2004)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 66912 Drosophila PIMRider - The Drosophila Protein Interaction map • 521499
Orthologs
Genome-wide drosophilid orthologs	Dana\GF11747 Dere\GG20737 Dgri\GH18747 Dmoj\GI24664 Dper\GL12069 Dpse\GA19554 Dsec\GM11488 Dsim\Cen190 Dvir\GJ24037 Dwil\GK13490 Dyak\Cen190
Curated drosophilid orthologs	Dmer\Cp190   Dpse\GA19554   Dsim\Cen190 (Begun, 2000.4.5) Dyak\Cen190 (Begun, 2000.4.7)
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000018397 Anopheles gambiae (Ensembl) ENSCINP00000014756 Ciona intestinalis (Ensembl)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
VDRC	v35077 w1118; P{GD11908}v35077 v35078 w1118; P{GD11908}v35078
Bloomington	22440 y1 w67c23; P{EPgy2}Cp190EY20997
Genomic Clones ( 2 )
	BACR13A02 BACR32A03
cDNA Clones ( 56 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD02352
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	SD01963 LD11188 LD09366 RE73077 RE56219 LD26683 LD30001 GM23328 RE04358 RE68874 SD13965 RE45084 LD28204 LD21770 LD06517 LD04376 LD27517 LD14803 LD21393 LD48072 LD47491 LD37194 LD13843 LD26695 HL01616 LD15565 SD02115 LD02848 SD05168 GM02009 LD47777 RE53080 RE05828 SD05172 RE5