Rb188, DMAP190, Cen190, CP-190, Centrosomal Protein 190
Gene model reviewed during 5.47
Low-frequency RNA-Seq exon junction(s) not annotated.
Component of the gypsy chromatin insulator complex, composed of Cp190, mod(mdg4) and su(Hw) (PubMed:15574329). The gypsy chromatin insulator complex interacts with Topors via mod(mdg4) and su(Hw) (PubMed:15574329). Interacts with Cp60 and microtubules (PubMed:8590797, PubMed:9700165, PubMed:8491775, PubMed:8522588, PubMed:14996941). Interacts with inv (PubMed:25561495). Interacts with Nup98 (PubMed:28366641).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cp190 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Cp190 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Cen190 CG6384
DNA-protein interactions: genome-wide binding profile assayed for Cp190 protein in 0-12 hr embryos; see Insulator_Class_I.mE01 collection report. Individual protein-binding experiments listed under "Samples" at GEO: 16245 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE16245).
The introduction of dsRNA made from templates generated with primers directed against this gene into S2 cells prevents the accumulation of Map60 to mitotic centrosomes.
Cp190 plays an essential role in flies that is independent of its association with centrosomes or microtubules.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Fluorescently labelled bacterially expressed Cp190 is injected into living embryos and protein behaviour during the rapid syncytial blastoderm divisions (nuclear cycles 10-13) is followed.
The localisation of Cp190 protein within the fertilised egg has been studied.
The region between amino acids 385 and 508 of the Cp190 protein is sufficient for good centrosomal localisation. A nuclear localisation domain is located between amino acids 201 and 271.
Cp190 is associated with the centrosome during mitosis and during interphase the protein is associated with a large number of specific chromosomal sites. Cp190 binds to chromatin in association with another centrosomal protein, Map60.
By using monoclonal antibodies that recognize protein components of the mitotic apparatus to screen expression libraries, Cen185, which encodes a protein associated with the centrosome, was cloned.