spalt, sal, sal-r
Gene model reviewed during 5.51
5 (northern blot)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\salr using the Feature Mapper tool.
Comment: reported as posterior spiracle specific anlage
salr is expressed at low levels in embryos, larvae, and pupae, and even lower levels in adults. Using RT-PCR, no salr transcript is detected in ovary or in S2 tissue culture cells. salr is present in older embryos, decreases in abundance by second larval instar, increases in third instar and pupae, and decreases again in the adult.
salr and salm transcripts and proteins are distributed in identicals patterns in the third larval instar wing disc. salr transcript expression in the wing disc is restricted to a band 4-6 cells wide, perpendicular to the dorsoventral boundary. The band is first detected in the dorsal compartment, and expands ventrally. In late third-instar larval discs expression of salr and salm occurs in a broader domain centered on the dpp stripe.
salr protein is not detected in early embryogenesis. Later in embryogenesis, expression is detected in a limited number of tissues, most notably the CNS. In larvae, expression continues to be detected in the CNS and is also detected in imaginal discs. salr and salm protein expression patterns were compared. salm and salr proteins have identical staining patterns in the wing imaginal disc (FBrf0087811), but have different patterns in the embryonic CNS and spiracles. salr is more abundant in the embryonic brain, while salm is more abundant in the posterior spiracle. The two proteins have overlapping but non-identical patterns in the ventral nerve cord.
GBrowse - Visual display of RNA-Seq signalsView Dmel\salr in GBrowse 2
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: salr CG4881
salm and salr participate in the development of sensory organs in the thorax, mainly in the positioning of specific proneural clusters. They may belong to a category of transcriptional regulators that subdivide the thorax into expression domains (prepattern) required for the localised activation of proneural genes.
Isolation and characterisation of salr, a gene arisen by reduplication and sequence diversification from the same ancestral gene as salm, that is still located close to salm in the genome. Sequence comparisons reveal a pattern of regional structural diversification of salm and salr. Electrophoretic mobility shift and footprinting assays demonstrate specific DNA binding of a conserved set of zinc fingers. The genes have extensive overlapping but not coincident patterns of expression in structures, such as the nervous system, indicating significant regulatory diversification.
salr and salm are expressed in response to dpp in a central territory of the wing imaginal disc, where they are required for the patterning of the wing. The phenotypic consequences of misexpression of salr and salm suggest that an important outcome of dpp activity is the subdivision of the wing disc into territories smaller than lineage compartments through the regulation of salr and salm.