Gene Dmel\Cg25C

FlyGene Wiki Help Close All Open All

General Information

Symbol

Dmel\Cg25C

Species

D. melanogaster

Name

Collagen type IV

Annotation symbol

CG4145

Feature type

protein_coding_gene

FlyBase ID

FBgn0000299

Gene Model Status

Current

Stock availability

14 publicly available

Also Known As

Dcg1

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

25C1-25C1

Sequence location

2L:5,029,609..5,037,279 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene Collagen type IV is referred to in FlyBase by the symbol Dmel\Cg25C (CG4145, FBgn0000299). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: extracellular matrix structural constituent. There is experimental evidence that it is involved in the biological process: oviduct morphogenesis. 49 alleles are reported. The phenotypes of these alleles are annotated with: intracellular membrane-bounded organelle; embryonic/larval visceral muscle; embryonic/larval brain; embryonic/larval optic lobe; head; embryonic/larval somatic muscle; mitochondrial membrane; embryonic/larval central nervous system. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: C-type lectin fold; Collagen IV, non-collagenous; Collagen triple helix repeat. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 12-24 hour embryonic stages, during early larval stages, during late pupal stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: adult thoracico-abdominal ganglion, adult heart, larval/adult fat body, adult spermathecae, adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, larval hindgut, larval salivary gland, larval trachea, adult ovary, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1630150_s_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of Cg25C. Gene sequence location is 2L:5029609..5037279.

User Contributed Data

Beta version, feedback welcome

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

Cg25C

Structural gene for a type IV basement-membrane collagen. Expression first detected following germ-band shortening in cells of both somatic and visceral mesodermal origin, specifically in mesoblasts (hemocytes) and fat-body cells (Mirre, Cecchini, LeParco, and Knibiehler, 1988, Development 102: 369-76). Translation takes place in embryonic mesoblasts and larval fat-body cells, and is deposited extracellularly in basement membranes surrounding skeletal and visceral muscles (LeParco, Bevic, Knibiehler, Mirre, and Cecchini, 1989, Insect Biochem. 19: 789-802).

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

Update Feed

Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.

FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

25C1-25C1

Limits computationally determined from genome sequence between P{lacW}vkg^k16721&P{lacW}Cg25C^k00405 and P{lacW}l(2)k11206^k11206

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

25C1-25C2

(determined by in situ hybridisation)

(Spradling et al., 1999)

25C5-25C6

(determined by in situ hybridisation)

(Cuenca et al., 1998)

25C-25C

(Gellon et al., 1997)

25C-25C

(determined by in situ hybridisation)

(Rodriguez et al., 1996)

25C-25C

(Kimbrell, 1996.4.28)

25C1-25C2

(BDGP Project Members, 1994-1999)

25C-25C

(determined by in situ hybridisation)

(Blumberg et al., 1988)

25C-25C

(determined by in situ hybridisation)

(Blumberg et al., 1987)

25C-25C

(determined by in situ hybridisation)

(Cecchini et al., 1987)

25C-25C

(determined by in situ hybridisation)

(Le Parco et al., 1986)

25C-25C

(determined by in situ hybridisation)

(Natzle and McCarthy, 1984)

25C-25C

(determined by in situ hybridisation)

(Monson et al., 1982)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\Cg25C for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Cg25C-RA

FBtr0079003

NM_164615

6000

1779

Cg25C-RB

FBtr0079002

NM_164616

6101

1779

Cg25C-RC

FBtr0079001

NM_164617

5635

1779

Additional Transcript Data & Comments

Reported size (kB)

6.3 (northern blot)

(Le Parco et al., 1986)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Cg25C-PA

FBpp0078642

174.3

1779

8.85

NP_723044

AAF52204

Cg25C-PB

FBpp0078641

174.3

1779

8.85

NP_723045

AAN10519

Cg25C-PC

FBpp0078640

174.3

1779

8.85

NP_723046

AAN10520

Additional Polypeptide Data & Comments

Reported size (kDa)

1775 (aa)

(Blumberg et al., 1988)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Collagen IV, non-collagenous (IPR001442)

Collagen triple helix repeat (IPR008160)

C-type lectin fold (IPR016187)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P08120

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

regulatory region

FBsf0000436109
2L:5,027,469..5,030,217

evidence=Reporter construct (in vivo)

(Asha et al., 2003, Halfon, 2012.6.19)

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 12

plasmatocyte primordium

(Fisher et al., 2012)

Comment:reported as plasmatocytes anlage

embryonic stage 13 -- 16

embryonic/larval fat body

(Fisher et al., 2012)

plasmatocyte

(Fisher et al., 2012)

embryonic stage 15 -- 16

embryonic/larval hemocyte

(Yasothornsrikul et al., 1997)

embryonic stage 16 -- 17

embryonic/larval fat body

(Yasothornsrikul et al., 1997)

first instar larval stage

embryonic/larval fat body

(Yasothornsrikul et al., 1997)

Additional Descriptive Data

Cg25C expression initiates later in embryogenesis than LanA and shows a different development profile. Cg25C transcripts are first detected in 6-8hr embryos and reach a plateau by 16-18hr. Levels remain high through the larval instars, decrease in late third instar, and increase throughout the last two hours before eclosion.

(Kusche-Gullberg et al., 1992)

Cg25C and vkg transcripts are both detected in fat bodies and hemocytes in late embryogenesis, and fat bodies in first larval instar.

(Yasothornsrikul et al., 1997)

Detected in embryonic, larval, pupal and adult RNA.

(Le Parco et al., 1986)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage P5

leg & joint

• plasma membrane | distal

(Mirth and Akam, 2002)

Comment:18 hr APF

pupal stage P6

leg & joint | distal

• plasma membrane | distal

(Mirth and Akam, 2002)

Comment:30 hr APF

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Protein is evenly distributed on distal cell surfaces throughout the leg at 18 h after pupation. At 30 h after pupation protein is concentrated on the basal surface of distal joint cells but is much weaker in other leg regions.

(Mirth and Akam, 2002)

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

Reporter: P{Cg-GAL4.A}

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

hemocyte

(Yoshida et al., 2001, Harikrishnan et al., 2000, Asha et al., 2003)

anterior lymph gland pair

(Asha et al., 2003)

fat body

(Takata et al., 2004, Harikrishnan et al., 2000, Asha et al., 2003)

Reporter: P{lacW}Cg25C^k13420

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval hemocyte

(Rodriguez et al., 1996)

embryonic stage -- larval stage & adult stage

fat body

(Rodriguez et al., 1996)

larval stage

embryonic/larval central nervous system

(Rodriguez et al., 1996)

Reporter: P{lArB}Cg25C^A109.1F2

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Matthews, 1995.11.17)

embryonic stage 17

embryonic/larval fat body

(Bellen et al., 1989)

hemocyte

(Bellen et al., 1989)

adult stage & oogenesis

follicle cell

(Bellen, 1998.8.10)

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: adult thoracico-abdominal ganglion, adult heart, larval/adult fat body, adult spermathecae, adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, larval hindgut, larval salivary gland, larval trachea, adult ovary, larval carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		280.775
Larval Midgut		41.2
Larval Hindgut		268.5
Larval Malpighian Tubules		55.2
Larval Fat Body		519.2
Larval Salivary Gland		126.9
Larval Trachea		229.325
Larval Carcass		326.75
Adult Head		338
Adult Eye		103.925
Adult Brain		369
Adult Thoracic-Abdominal Ganglion		710
Adult Crop		49.4
Adult Midgut		20.2
Adult Hindgut		64.5
Adult Malpighian Tubules		29.9
Adult Fat Body		1374.6
Adult Salivary Gland		15.2
Adult Heart		1899.825
Adult VirginFemale Spermatheca		658
Adult InseminatedFemale Spermatheca		1035.8
Adult Ovary		155.4
Adult Testis		44.8
Adult Male Accessory Gland		39.6
Adult Carcass		606.9
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		280.775
Larval Midgut		41.2
Larval Hindgut		268.5
Larval Malpighian Tubules		55.2
Larval Fat Body		519.2
Larval Salivary Gland		126.9
Larval Trachea		229.325
Larval Carcass		326.75
Adult Head		338
Adult Eye		103.925
Adult Brain		369
Adult Thoracic-Abdominal Ganglion		(710)
Adult Crop		49.4
Adult Midgut		20.2
Adult Hindgut		64.5
Adult Malpighian Tubules		29.9
Adult Fat Body		(1374.6)
Adult Salivary Gland		15.2
Adult Heart		(1899.825)
Adult VirginFemale Spermatheca		(658)
Adult InseminatedFemale Spermatheca		(1035.8)
Adult Ovary		155.4
Adult Testis		44.8
Adult Male Accessory Gland		39.6
Adult Carcass		(606.9)
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		280.775
Larval Midgut		41.2
Larval Hindgut		268.5
Larval Malpighian Tubules		55.2
Larval Fat Body		519.2
Larval Salivary Gland		126.9
Larval Trachea		229.325
Larval Carcass		326.75
Adult Head		338
Adult Eye		103.925
Adult Brain		369
Adult Thoracic-Abdominal Ganglion		710
Adult Crop		49.4
Adult Midgut		20.2
Adult Hindgut		64.5
Adult Malpighian Tubules		29.9
Adult Fat Body		(1374.6)
Adult Salivary Gland		15.2
Adult Heart		(1899.825)
Adult VirginFemale Spermatheca		658
Adult InseminatedFemale Spermatheca		1035.8
Adult Ovary		155.4
Adult Testis		44.8
Adult Male Accessory Gland		39.6
Adult Carcass		606.9
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		280.775
Larval Midgut		41.2
Larval Hindgut		268.5
Larval Malpighian Tubules		55.2
Larval Fat Body		519.2
Larval Salivary Gland		126.9
Larval Trachea		229.325
Larval Carcass		326.75
Adult Head		338
Adult Eye		103.925
Adult Brain		369
Adult Thoracic-Abdominal Ganglion		710
Adult Crop		49.4
Adult Midgut		20.2
Adult Hindgut		64.5
Adult Malpighian Tubules		29.9
Adult Fat Body		1374.6
Adult Salivary Gland		15.2
Adult Heart		1899.825
Adult VirginFemale Spermatheca		658
Adult InseminatedFemale Spermatheca		1035.8
Adult Ovary		155.4
Adult Testis		44.8
Adult Male Accessory Gland		39.6
Adult Carcass		606.9
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		280.775
Larval Midgut		41.2
Larval Hindgut		268.5
Larval Malpighian Tubules		55.2
Larval Fat Body		519.2
Larval Salivary Gland		126.9
Larval Trachea		229.325
Larval Carcass		326.75
Adult Head		338
Adult Eye		103.925
Adult Brain		369
Adult Thoracic-Abdominal Ganglion		710
Adult Crop		49.4
Adult Midgut		20.2
Adult Hindgut		64.5
Adult Malpighian Tubules		29.9
Adult Fat Body		1374.6
Adult Salivary Gland		15.2
Adult Heart		1899.825
Adult VirginFemale Spermatheca		658
Adult InseminatedFemale Spermatheca		1035.8
Adult Ovary		155.4
Adult Testis		44.8
Adult Male Accessory Gland		39.6
Adult Carcass		606.9
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		280.775
Larval Midgut		41.2
Larval Hindgut		268.5
Larval Malpighian Tubules		55.2
Larval Fat Body		519.2
Larval Salivary Gland		126.9
Larval Trachea		229.325
Larval Carcass		326.75
Adult Head		338
Adult Eye		103.925
Adult Brain		369
Adult Thoracic-Abdominal Ganglion		710
Adult Crop		49.4
Adult Midgut		20.2
Adult Hindgut		64.5
Adult Malpighian Tubules		29.9
Adult Fat Body		(1374.6)
Adult Salivary Gland		15.2
Adult Heart		(1899.825)
Adult VirginFemale Spermatheca		658
Adult InseminatedFemale Spermatheca		(1035.8)
Adult Ovary		155.4
Adult Testis		44.8
Adult Male Accessory Gland		39.6
Adult Carcass		606.9
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		280.775
Larval Midgut		41.2
Larval Hindgut		268.5
Larval Malpighian Tubules		55.2
Larval Fat Body		519.2
Larval Salivary Gland		126.9
Larval Trachea		229.325
Larval Carcass		326.75
Adult Head		338
Adult Eye		103.925
Adult Brain		369
Adult Thoracic-Abdominal Ganglion		710
Adult Crop		49.4
Adult Midgut		20.2
Adult Hindgut		64.5
Adult Malpighian Tubules		29.9
Adult Fat Body		1374.6
Adult Salivary Gland		15.2
Adult Heart		(1899.825)
Adult VirginFemale Spermatheca		658
Adult InseminatedFemale Spermatheca		1035.8
Adult Ovary		155.4
Adult Testis		44.8
Adult Male Accessory Gland		39.6
Adult Carcass		606.9
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		280.775
Larval Midgut		41.2
Larval Hindgut		268.5
Larval Malpighian Tubules		55.2
Larval Fat Body		519.2
Larval Salivary Gland		126.9
Larval Trachea		229.325
Larval Carcass		326.75
Adult Head		338
Adult Eye		103.925
Adult Brain		369
Adult Thoracic-Abdominal Ganglion		710
Adult Crop		49.4
Adult Midgut		20.2
Adult Hindgut		64.5
Adult Malpighian Tubules		29.9
Adult Fat Body		1374.6
Adult Salivary Gland		15.2
Adult Heart		1899.825
Adult VirginFemale Spermatheca		658
Adult InseminatedFemale Spermatheca		1035.8
Adult Ovary		155.4
Adult Testis		44.8
Adult Male Accessory Gland		39.6
Adult Carcass		606.9
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	338
NA	Eye	103.925
NA	Brain	369
280.775	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	710
NA	Crop	49.4
41.2	Midgut	no informative data
268.5	Hindgut	64.5
55.2	Malpighian Tubules	no informative data
519.2	Fat Body	1374.6
126.9	Salivary Gland	no informative data
NA	Heart	1899.825
229.325	Trachea	NA
NA	VirginFemale Spermatheca	658
NA	InseminatedFemale Spermatheca	1035.8
NA	Ovary	155.4
NA	Testis	44.8
NA	Male Accessory Gland	39.6
326.75	Carcass	606.9

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		12
central nervous system, larvae L3		14
central nervous system, pupae P8		57
head, virgin 1-day female		186
head, virgin 4-day female		88
head, virgin 20-day female		43
head, mated 1-day female		170
head, mated 4-day female		90
head, mated 20-day female		55
head, mated 1-day male		152
head, mated 4-day male		95
head, mated 20-day male		54
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		30
digestive system, 4-day adult		13
digestive system, 20-day adult		6
fat body, larvae L3 wandering		313
fat body, white prepupae		40
fat body, pupae P8		122
carcass, larvae L3 wandering		24
carcass, 1-day adult		188
carcass, 4-day adult		111
carcass, 20-day adult		77
ovary, virgin 4-day female		23
ovary, mated 4-day female		22
testis, mated 4-day male		5
accessory gland, mated 4-day male		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		12
central nervous system, larvae L3		14
central nervous system, pupae P8		(57)
head, virgin 1-day female		(186)
head, virgin 4-day female		(88)
head, virgin 20-day female		(43)
head, mated 1-day female		(170)
head, mated 4-day female		(90)
head, mated 20-day female		(55)
head, mated 1-day male		(152)
head, mated 4-day male		(95)
head, mated 20-day male		(54)
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		(30)
digestive system, 4-day adult		13
digestive system, 20-day adult		6
fat body, larvae L3 wandering		(313)
fat body, white prepupae		(40)
fat body, pupae P8		(122)
carcass, larvae L3 wandering		24
carcass, 1-day adult		(188)
carcass, 4-day adult		(111)
carcass, 20-day adult		(77)
ovary, virgin 4-day female		23
ovary, mated 4-day female		22
testis, mated 4-day male		5
accessory gland, mated 4-day male		7
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		12
central nervous system, larvae L3		14
central nervous system, pupae P8		57
head, virgin 1-day female		(186)
head, virgin 4-day female		88
head, virgin 20-day female		43
head, mated 1-day female		(170)
head, mated 4-day female		90
head, mated 20-day female		55
head, mated 1-day male		(152)
head, mated 4-day male		95
head, mated 20-day male		54
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		30
digestive system, 4-day adult		13
digestive system, 20-day adult		6
fat body, larvae L3 wandering		(313)
fat body, white prepupae		40
fat body, pupae P8		(122)
carcass, larvae L3 wandering		24
carcass, 1-day adult		(188)
carcass, 4-day adult		(111)
carcass, 20-day adult		77
ovary, virgin 4-day female		23
ovary, mated 4-day female		22
testis, mated 4-day male		5
accessory gland, mated 4-day male		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		12
central nervous system, larvae L3		14
central nervous system, pupae P8		57
head, virgin 1-day female		186
head, virgin 4-day female		88
head, virgin 20-day female		43
head, mated 1-day female		170
head, mated 4-day female		90
head, mated 20-day female		55
head, mated 1-day male		152
head, mated 4-day male		95
head, mated 20-day male		54
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		30
digestive system, 4-day adult		13
digestive system, 20-day adult		6
fat body, larvae L3 wandering		313
fat body, white prepupae		40
fat body, pupae P8		122
carcass, larvae L3 wandering		24
carcass, 1-day adult		188
carcass, 4-day adult		111
carcass, 20-day adult		77
ovary, virgin 4-day female		23
ovary, mated 4-day female		22
testis, mated 4-day male		5
accessory gland, mated 4-day male		7
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		12
central nervous system, larvae L3		14
central nervous system, pupae P8		57
head, virgin 1-day female		186
head, virgin 4-day female		88
head, virgin 20-day female		43
head, mated 1-day female		170
head, mated 4-day female		90
head, mated 20-day female		55
head, mated 1-day male		152
head, mated 4-day male		95
head, mated 20-day male		54
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		30
digestive system, 4-day adult		13
digestive system, 20-day adult		6
fat body, larvae L3 wandering		313
fat body, white prepupae		40
fat body, pupae P8		122
carcass, larvae L3 wandering		24
carcass, 1-day adult		188
carcass, 4-day adult		111
carcass, 20-day adult		77
ovary, virgin 4-day female		23
ovary, mated 4-day female		22
testis, mated 4-day male		5
accessory gland, mated 4-day male		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		12
central nervous system, larvae L3		14
central nervous system, pupae P8		(57)
head, virgin 1-day female		(186)
head, virgin 4-day female		(88)
head, virgin 20-day female		(43)
head, mated 1-day female		(170)
head, mated 4-day female		(90)
head, mated 20-day female		(55)
head, mated 1-day male		(152)
head, mated 4-day male		(95)
head, mated 20-day male		(54)
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		30
digestive system, 4-day adult		13
digestive system, 20-day adult		6
fat body, larvae L3 wandering		(313)
fat body, white prepupae		(40)
fat body, pupae P8		(122)
carcass, larvae L3 wandering		24
carcass, 1-day adult		(188)
carcass, 4-day adult		(111)
carcass, 20-day adult		(77)
ovary, virgin 4-day female		23
ovary, mated 4-day female		22
testis, mated 4-day male		5
accessory gland, mated 4-day male		7
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		12
central nervous system, larvae L3		14
central nervous system, pupae P8		57
head, virgin 1-day female		(186)
head, virgin 4-day female		88
head, virgin 20-day female		43
head, mated 1-day female		(170)
head, mated 4-day female		90
head, mated 20-day female		55
head, mated 1-day male		(152)
head, mated 4-day male		95
head, mated 20-day male		54
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		30
digestive system, 4-day adult		13
digestive system, 20-day adult		6
fat body, larvae L3 wandering		(313)
fat body, white prepupae		40
fat body, pupae P8		122
carcass, larvae L3 wandering		24
carcass, 1-day adult		(188)
carcass, 4-day adult		111
carcass, 20-day adult		77
ovary, virgin 4-day female		23
ovary, mated 4-day female		22
testis, mated 4-day male		5
accessory gland, mated 4-day male		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		12
central nervous system, larvae L3		14
central nervous system, pupae P8		57
head, virgin 1-day female		186
head, virgin 4-day female		88
head, virgin 20-day female		43
head, mated 1-day female		170
head, mated 4-day female		90
head, mated 20-day female		55
head, mated 1-day male		152
head, mated 4-day male		95
head, mated 20-day male		54
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		30
digestive system, 4-day adult		13
digestive system, 20-day adult		6
fat body, larvae L3 wandering		313
fat body, white prepupae		40
fat body, pupae P8		122
carcass, larvae L3 wandering		24
carcass, 1-day adult		188
carcass, 4-day adult		111
carcass, 20-day adult		77
ovary, virgin 4-day female		23
ovary, mated 4-day female		22
testis, mated 4-day male		5
accessory gland, mated 4-day male		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 12-24 hour embryonic stages, during early larval stages, during late pupal stages, in adult female stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		2
embryo 02-04hr		2
embryo 04-06hr		2
embryo 06-08hr		10
embryo 08-10hr		18
embryo 10-12hr		52
embryo 12-14hr		99
embryo 14-16hr		425
embryo 16-18hr		727
embryo 18-20hr		1194
embryo 20-22hr		869
embryo 22-24hr		699
larva L1		566
larva L2		616
larva L3 12hr old		675
larva L3 puffstage 1-2		85
larva L3 puffstage 3-6		55
larva L3 puffstage 7-9		22
white prepupae new		22
white prepupae 12hr		25
white prepupae 24hr		20
pupae 2d postWPP		47
pupae 3d postWPP		165
pupae 4d postWPP		117
adult male 01day		105
adult male 05day		42
adult male 30day		16
adult female 01day		131
adult female 05day		47
adult female 30day		43
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		2
embryo 02-04hr		2
embryo 04-06hr		2
embryo 06-08hr		10
embryo 08-10hr		18
embryo 10-12hr		(52)
embryo 12-14hr		(99)
embryo 14-16hr		(425)
embryo 16-18hr		(727)
embryo 18-20hr		(1194)
embryo 20-22hr		(869)
embryo 22-24hr		(699)
larva L1		(566)
larva L2		(616)
larva L3 12hr old		(675)
larva L3 puffstage 1-2		(85)
larva L3 puffstage 3-6		(55)
larva L3 puffstage 7-9		22
white prepupae new		22
white prepupae 12hr		25
white prepupae 24hr		20
pupae 2d postWPP		(47)
pupae 3d postWPP		(165)
pupae 4d postWPP		(117)
adult male 01day		(105)
adult male 05day		(42)
adult male 30day		16
adult female 01day		(131)
adult female 05day		(47)
adult female 30day		(43)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		2
embryo 02-04hr		2
embryo 04-06hr		2
embryo 06-08hr		10
embryo 08-10hr		18
embryo 10-12hr		52
embryo 12-14hr		99
embryo 14-16hr		(425)
embryo 16-18hr		(727)
embryo 18-20hr		(1194)
embryo 20-22hr		(869)
embryo 22-24hr		(699)
larva L1		(566)
larva L2		(616)
larva L3 12hr old		(675)
larva L3 puffstage 1-2		85
larva L3 puffstage 3-6		55
larva L3 puffstage 7-9		22
white prepupae new		22
white prepupae 12hr		25
white prepupae 24hr		20
pupae 2d postWPP		47
pupae 3d postWPP		(165)
pupae 4d postWPP		(117)
adult male 01day		105
adult male 05day		42
adult male 30day		16
adult female 01day		(131)
adult female 05day		47
adult female 30day		43
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		2
embryo 02-04hr		2
embryo 04-06hr		2
embryo 06-08hr		10
embryo 08-10hr		18
embryo 10-12hr		52
embryo 12-14hr		99
embryo 14-16hr		425
embryo 16-18hr		727
embryo 18-20hr		1194
embryo 20-22hr		869
embryo 22-24hr		699
larva L1		566
larva L2		616
larva L3 12hr old		675
larva L3 puffstage 1-2		85
larva L3 puffstage 3-6		55
larva L3 puffstage 7-9		22
white prepupae new		22
white prepupae 12hr		25
white prepupae 24hr		20
pupae 2d postWPP		47
pupae 3d postWPP		165
pupae 4d postWPP		117
adult male 01day		105
adult male 05day		42
adult male 30day		16
adult female 01day		131
adult female 05day		47
adult female 30day		43
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		2
embryo 02-04hr		2
embryo 04-06hr		2
embryo 06-08hr		10
embryo 08-10hr		18
embryo 10-12hr		52
embryo 12-14hr		99
embryo 14-16hr		425
embryo 16-18hr		727
embryo 18-20hr		1194
embryo 20-22hr		869
embryo 22-24hr		699
larva L1		566
larva L2		616
larva L3 12hr old		675
larva L3 puffstage 1-2		85
larva L3 puffstage 3-6		55
larva L3 puffstage 7-9		22
white prepupae new		22
white prepupae 12hr		25
white prepupae 24hr		20
pupae 2d postWPP		47
pupae 3d postWPP		165
pupae 4d postWPP		117
adult male 01day		105
adult male 05day		42
adult male 30day		16
adult female 01day		131
adult female 05day		47
adult female 30day		43
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		2
embryo 02-04hr		2
embryo 04-06hr		2
embryo 06-08hr		10
embryo 08-10hr		18
embryo 10-12hr		(52)
embryo 12-14hr		(99)
embryo 14-16hr		(425)
embryo 16-18hr		(727)
embryo 18-20hr		(1194)
embryo 20-22hr		(869)
embryo 22-24hr		(699)
larva L1		(566)
larva L2		(616)
larva L3 12hr old		(675)
larva L3 puffstage 1-2		(85)
larva L3 puffstage 3-6		(55)
larva L3 puffstage 7-9		22
white prepupae new		22
white prepupae 12hr		25
white prepupae 24hr		20
pupae 2d postWPP		(47)
pupae 3d postWPP		(165)
pupae 4d postWPP		(117)
adult male 01day		(105)
adult male 05day		(42)
adult male 30day		16
adult female 01day		(131)
adult female 05day		(47)
adult female 30day		(43)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		2
embryo 02-04hr		2
embryo 04-06hr		2
embryo 06-08hr		10
embryo 08-10hr		18
embryo 10-12hr		52
embryo 12-14hr		99
embryo 14-16hr		(425)
embryo 16-18hr		(727)
embryo 18-20hr		(1194)
embryo 20-22hr		(869)
embryo 22-24hr		(699)
larva L1		(566)
larva L2		(616)
larva L3 12hr old		(675)
larva L3 puffstage 1-2		85
larva L3 puffstage 3-6		55
larva L3 puffstage 7-9		22
white prepupae new		22
white prepupae 12hr		25
white prepupae 24hr		20
pupae 2d postWPP		47
pupae 3d postWPP		(165)
pupae 4d postWPP		117
adult male 01day		105
adult male 05day		42
adult male 30day		16
adult female 01day		131
adult female 05day		47
adult female 30day		43
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		2
embryo 02-04hr		2
embryo 04-06hr		2
embryo 06-08hr		10
embryo 08-10hr		18
embryo 10-12hr		52
embryo 12-14hr		99
embryo 14-16hr		425
embryo 16-18hr		727
embryo 18-20hr		1194
embryo 20-22hr		869
embryo 22-24hr		699
larva L1		566
larva L2		616
larva L3 12hr old		675
larva L3 puffstage 1-2		85
larva L3 puffstage 3-6		55
larva L3 puffstage 7-9		22
white prepupae new		22
white prepupae 12hr		25
white prepupae 24hr		20
pupae 2d postWPP		47
pupae 3d postWPP		165
pupae 4d postWPP		117
adult male 01day		105
adult male 05day		42
adult male 30day		16
adult female 01day		131
adult female 05day		47
adult female 30day		43
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		229
Schneider line 2 Sg4		2492
embryonic 1182-4H		465
embryonic GM2		216
embryonic Kc167		98
embryonic S1		23
embryonic S3		45
leg disc CME L1		2496
wing disc CME-W2		144
wing disc ML-DmD8		344
wing disc ML-DmD9		338
wing disc ML-DmD16-c3		511
wing disc ML-DmD21		109
wing disc ML-DmD32		2011
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		1149
antennal disc ML-DmD20-c5		144
mixed discs ML-DmD4-c1		1241
CNS ML-DmBG1-c1		6
CNS ML-DmBG2-c2		128
tumorous blood cells mbn2		9
ovary fGS/OSS		240
ovary OSC		12
ovary OSS		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(229)
Schneider line 2 Sg4		(2492)
embryonic 1182-4H		(465)
embryonic GM2		(216)
embryonic Kc167		(98)
embryonic S1		23
embryonic S3		(45)
leg disc CME L1		(2496)
wing disc CME-W2		(144)
wing disc ML-DmD8		(344)
wing disc ML-DmD9		(338)
wing disc ML-DmD16-c3		(511)
wing disc ML-DmD21		(109)
wing disc ML-DmD32		(2011)
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		(1149)
antennal disc ML-DmD20-c5		(144)
mixed discs ML-DmD4-c1		(1241)
CNS ML-DmBG1-c1		6
CNS ML-DmBG2-c2		(128)
tumorous blood cells mbn2		9
ovary fGS/OSS		(240)
ovary OSC		12
ovary OSS		9
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		(229)
Schneider line 2 Sg4		(2492)
embryonic 1182-4H		(465)
embryonic GM2		(216)
embryonic Kc167		98
embryonic S1		23
embryonic S3		45
leg disc CME L1		(2496)
wing disc CME-W2		(144)
wing disc ML-DmD8		(344)
wing disc ML-DmD9		(338)
wing disc ML-DmD16-c3		(511)
wing disc ML-DmD21		(109)
wing disc ML-DmD32		(2011)
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		(1149)
antennal disc ML-DmD20-c5		(144)
mixed discs ML-DmD4-c1		(1241)
CNS ML-DmBG1-c1		6
CNS ML-DmBG2-c2		(128)
tumorous blood cells mbn2		9
ovary fGS/OSS		(240)
ovary OSC		12
ovary OSS		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		229
Schneider line 2 Sg4		2492
embryonic 1182-4H		465
embryonic GM2		216
embryonic Kc167		98
embryonic S1		23
embryonic S3		45
leg disc CME L1		2496
wing disc CME-W2		144
wing disc ML-DmD8		344
wing disc ML-DmD9		338
wing disc ML-DmD16-c3		511
wing disc ML-DmD21		109
wing disc ML-DmD32		2011
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		1149
antennal disc ML-DmD20-c5		144
mixed discs ML-DmD4-c1		1241
CNS ML-DmBG1-c1		6
CNS ML-DmBG2-c2		128
tumorous blood cells mbn2		9
ovary fGS/OSS		240
ovary OSC		12
ovary OSS		9
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		229
Schneider line 2 Sg4		2492
embryonic 1182-4H		465
embryonic GM2		216
embryonic Kc167		98
embryonic S1		23
embryonic S3		45
leg disc CME L1		2496
wing disc CME-W2		144
wing disc ML-DmD8		344
wing disc ML-DmD9		338
wing disc ML-DmD16-c3		511
wing disc ML-DmD21		109
wing disc ML-DmD32		2011
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		1149
antennal disc ML-DmD20-c5		144
mixed discs ML-DmD4-c1		1241
CNS ML-DmBG1-c1		6
CNS ML-DmBG2-c2		128
tumorous blood cells mbn2		9
ovary fGS/OSS		240
ovary OSC		12
ovary OSS		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(229)
Schneider line 2 Sg4		(2492)
embryonic 1182-4H		(465)
embryonic GM2		(216)
embryonic Kc167		(98)
embryonic S1		23
embryonic S3		(45)
leg disc CME L1		(2496)
wing disc CME-W2		(144)
wing disc ML-DmD8		(344)
wing disc ML-DmD9		(338)
wing disc ML-DmD16-c3		(511)
wing disc ML-DmD21		(109)
wing disc ML-DmD32		(2011)
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		(1149)
antennal disc ML-DmD20-c5		(144)
mixed discs ML-DmD4-c1		(1241)
CNS ML-DmBG1-c1		6
CNS ML-DmBG2-c2		(128)
tumorous blood cells mbn2		9
ovary fGS/OSS		(240)
ovary OSC		12
ovary OSS		9
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		(229)
Schneider line 2 Sg4		(2492)
embryonic 1182-4H		(465)
embryonic GM2		(216)
embryonic Kc167		98
embryonic S1		23
embryonic S3		45
leg disc CME L1		(2496)
wing disc CME-W2		(144)
wing disc ML-DmD8		(344)
wing disc ML-DmD9		(338)
wing disc ML-DmD16-c3		(511)
wing disc ML-DmD21		109
wing disc ML-DmD32		(2011)
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		(1149)
antennal disc ML-DmD20-c5		(144)
mixed discs ML-DmD4-c1		(1241)
CNS ML-DmBG1-c1		6
CNS ML-DmBG2-c2		128
tumorous blood cells mbn2		9
ovary fGS/OSS		(240)
ovary OSC		12
ovary OSS		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		229
Schneider line 2 Sg4		2492
embryonic 1182-4H		465
embryonic GM2		216
embryonic Kc167		98
embryonic S1		23
embryonic S3		45
leg disc CME L1		2496
wing disc CME-W2		144
wing disc ML-DmD8		344
wing disc ML-DmD9		338
wing disc ML-DmD16-c3		511
wing disc ML-DmD21		109
wing disc ML-DmD32		2011
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		1149
antennal disc ML-DmD20-c5		144
mixed discs ML-DmD4-c1		1241
CNS ML-DmBG1-c1		6
CNS ML-DmBG2-c2		128
tumorous blood cells mbn2		9
ovary fGS/OSS		240
ovary OSC		12
ovary OSS		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		37
cold shock, 4-day adult		32
heat shock, 4-day adult		93
Cadmium 50 mM 6 hrs, larvae L3		107
Cadmium 50 mM 12 hrs, larvae L3		21
Cadmium 50 mM 48 hrs, 4-day adult		119
Cadmium 100 mM 48 hrs, 4-day adult		18
Copper 0.5 mM 12 hrs, larvae L3		180
Copper 15 mM 48 hrs, 4-day adult		37
Zinc 5 mM 12 hrs, larvae L3		127
Zinc 4.5 mM 48 hrs, 4-day adult		72
Ethanol 2.5% 3 hrs, larvae L3		22
Ethanol 5% 3 hrs, larvae L3		108
Ethanol 10% 3 hrs, larvae L3		121
Caffeine 1.5 mg/ml 4 hrs, larvae L3		71
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		30
Caffeine 25 mg/ml 48 hrs, 4-day adult		48
Paraquat 5 mM 48 hrs, 4-day adult		51
Paraquat 10 mM 48 hrs, 4-day adult		59
Rotenone 2 μg 12 hrs, larvae L3		86
Rotenone 8 μg 12 hrs, larvae L3		242
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(37)
cold shock, 4-day adult		(32)
heat shock, 4-day adult		(93)
Cadmium 50 mM 6 hrs, larvae L3		(107)
Cadmium 50 mM 12 hrs, larvae L3		21
Cadmium 50 mM 48 hrs, 4-day adult		(119)
Cadmium 100 mM 48 hrs, 4-day adult		18
Copper 0.5 mM 12 hrs, larvae L3		(180)
Copper 15 mM 48 hrs, 4-day adult		(37)
Zinc 5 mM 12 hrs, larvae L3		(127)
Zinc 4.5 mM 48 hrs, 4-day adult		(72)
Ethanol 2.5% 3 hrs, larvae L3		22
Ethanol 5% 3 hrs, larvae L3		(108)
Ethanol 10% 3 hrs, larvae L3		(121)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(71)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(30)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(48)
Paraquat 5 mM 48 hrs, 4-day adult		(51)
Paraquat 10 mM 48 hrs, 4-day adult		(59)
Rotenone 2 μg 12 hrs, larvae L3		(86)
Rotenone 8 μg 12 hrs, larvae L3		(242)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		37
cold shock, 4-day adult		32
heat shock, 4-day adult		93
Cadmium 50 mM 6 hrs, larvae L3		107
Cadmium 50 mM 12 hrs, larvae L3		21
Cadmium 50 mM 48 hrs, 4-day adult		(119)
Cadmium 100 mM 48 hrs, 4-day adult		18
Copper 0.5 mM 12 hrs, larvae L3		(180)
Copper 15 mM 48 hrs, 4-day adult		37
Zinc 5 mM 12 hrs, larvae L3		(127)
Zinc 4.5 mM 48 hrs, 4-day adult		72
Ethanol 2.5% 3 hrs, larvae L3		22
Ethanol 5% 3 hrs, larvae L3		(108)
Ethanol 10% 3 hrs, larvae L3		(121)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		71
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		30
Caffeine 25 mg/ml 48 hrs, 4-day adult		48
Paraquat 5 mM 48 hrs, 4-day adult		51
Paraquat 10 mM 48 hrs, 4-day adult		59
Rotenone 2 μg 12 hrs, larvae L3		86
Rotenone 8 μg 12 hrs, larvae L3		(242)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		37
cold shock, 4-day adult		32
heat shock, 4-day adult		93
Cadmium 50 mM 6 hrs, larvae L3		107
Cadmium 50 mM 12 hrs, larvae L3		21
Cadmium 50 mM 48 hrs, 4-day adult		119
Cadmium 100 mM 48 hrs, 4-day adult		18
Copper 0.5 mM 12 hrs, larvae L3		180
Copper 15 mM 48 hrs, 4-day adult		37
Zinc 5 mM 12 hrs, larvae L3		127
Zinc 4.5 mM 48 hrs, 4-day adult		72
Ethanol 2.5% 3 hrs, larvae L3		22
Ethanol 5% 3 hrs, larvae L3		108
Ethanol 10% 3 hrs, larvae L3		121
Caffeine 1.5 mg/ml 4 hrs, larvae L3		71
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		30
Caffeine 25 mg/ml 48 hrs, 4-day adult		48
Paraquat 5 mM 48 hrs, 4-day adult		51
Paraquat 10 mM 48 hrs, 4-day adult		59
Rotenone 2 μg 12 hrs, larvae L3		86
Rotenone 8 μg 12 hrs, larvae L3		242
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		37
cold shock, 4-day adult		32
heat shock, 4-day adult		93
Cadmium 50 mM 6 hrs, larvae L3		107
Cadmium 50 mM 12 hrs, larvae L3		21
Cadmium 50 mM 48 hrs, 4-day adult		119
Cadmium 100 mM 48 hrs, 4-day adult		18
Copper 0.5 mM 12 hrs, larvae L3		180
Copper 15 mM 48 hrs, 4-day adult		37
Zinc 5 mM 12 hrs, larvae L3		127
Zinc 4.5 mM 48 hrs, 4-day adult		72
Ethanol 2.5% 3 hrs, larvae L3		22
Ethanol 5% 3 hrs, larvae L3		108
Ethanol 10% 3 hrs, larvae L3		121
Caffeine 1.5 mg/ml 4 hrs, larvae L3		71
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		30
Caffeine 25 mg/ml 48 hrs, 4-day adult		48
Paraquat 5 mM 48 hrs, 4-day adult		51
Paraquat 10 mM 48 hrs, 4-day adult		59
Rotenone 2 μg 12 hrs, larvae L3		86
Rotenone 8 μg 12 hrs, larvae L3		242
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(37)
cold shock, 4-day adult		32
heat shock, 4-day adult		(93)
Cadmium 50 mM 6 hrs, larvae L3		(107)
Cadmium 50 mM 12 hrs, larvae L3		21
Cadmium 50 mM 48 hrs, 4-day adult		(119)
Cadmium 100 mM 48 hrs, 4-day adult		18
Copper 0.5 mM 12 hrs, larvae L3		(180)
Copper 15 mM 48 hrs, 4-day adult		(37)
Zinc 5 mM 12 hrs, larvae L3		(127)
Zinc 4.5 mM 48 hrs, 4-day adult		(72)
Ethanol 2.5% 3 hrs, larvae L3		22
Ethanol 5% 3 hrs, larvae L3		(108)
Ethanol 10% 3 hrs, larvae L3		(121)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(71)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		30
Caffeine 25 mg/ml 48 hrs, 4-day adult		(48)
Paraquat 5 mM 48 hrs, 4-day adult		(51)
Paraquat 10 mM 48 hrs, 4-day adult		(59)
Rotenone 2 μg 12 hrs, larvae L3		(86)
Rotenone 8 μg 12 hrs, larvae L3		(242)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		37
cold shock, 4-day adult		32
heat shock, 4-day adult		93
Cadmium 50 mM 6 hrs, larvae L3		107
Cadmium 50 mM 12 hrs, larvae L3		21
Cadmium 50 mM 48 hrs, 4-day adult		119
Cadmium 100 mM 48 hrs, 4-day adult		18
Copper 0.5 mM 12 hrs, larvae L3		(180)
Copper 15 mM 48 hrs, 4-day adult		37
Zinc 5 mM 12 hrs, larvae L3		127
Zinc 4.5 mM 48 hrs, 4-day adult		72
Ethanol 2.5% 3 hrs, larvae L3		22
Ethanol 5% 3 hrs, larvae L3		108
Ethanol 10% 3 hrs, larvae L3		121
Caffeine 1.5 mg/ml 4 hrs, larvae L3		71
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		30
Caffeine 25 mg/ml 48 hrs, 4-day adult		48
Paraquat 5 mM 48 hrs, 4-day adult		51
Paraquat 10 mM 48 hrs, 4-day adult		59
Rotenone 2 μg 12 hrs, larvae L3		86
Rotenone 8 μg 12 hrs, larvae L3		(242)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		37
cold shock, 4-day adult		32
heat shock, 4-day adult		93
Cadmium 50 mM 6 hrs, larvae L3		107
Cadmium 50 mM 12 hrs, larvae L3		21
Cadmium 50 mM 48 hrs, 4-day adult		119
Cadmium 100 mM 48 hrs, 4-day adult		18
Copper 0.5 mM 12 hrs, larvae L3		180
Copper 15 mM 48 hrs, 4-day adult		37
Zinc 5 mM 12 hrs, larvae L3		127
Zinc 4.5 mM 48 hrs, 4-day adult		72
Ethanol 2.5% 3 hrs, larvae L3		22
Ethanol 5% 3 hrs, larvae L3		108
Ethanol 10% 3 hrs, larvae L3		121
Caffeine 1.5 mg/ml 4 hrs, larvae L3		71
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		30
Caffeine 25 mg/ml 48 hrs, 4-day adult		48
Paraquat 5 mM 48 hrs, 4-day adult		51
Paraquat 10 mM 48 hrs, 4-day adult		59
Rotenone 2 μg 12 hrs, larvae L3		86
Rotenone 8 μg 12 hrs, larvae L3		242
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0000299

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG4145-RC

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Cg25C

Stages(s) 11-12

Stages(s) 13-16

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal (with Cg25C^b-9)

lethal (with Cg25C^b-29)

Cg25C^b-9

lethal (with Cg25C^h-13)

Cg25C^b-9

lethal (with Df(2L)Exel7022)

Cg25C^k00405

lethal | dominant | cold sensitive

lethal | dominant | heat sensitive

lethal | dominant | partially | heat sensitive

lethal | larval stage, with Scer\GAL4^Mef2.PR

Cg25C^KK109933

partially lethal - majority die | dominant | embryonic stage | heat sensitive

viable

Cg25C^EY15460

viable, with Scer\GAL4^pnr-MD237

Cg25C^GD12784

viable | partially

Cg25C^del.hs

Sterility

Allele

female sterile | dominant | heat sensitive

Cg25C^DTS-L3

fertile

Cg25C^EY15460

Other Phenotypes

Allele

locomotor behavior defective | dominant | third instar larval stage | heat sensitive

Cg25C^DTS-L3

neuroanatomy defective | larval stage

Cg25C^del.hs

small body | larval stage, with Scer\GAL4^Mef2.PR

Cg25C^KK109933

Phenotype manifest in

Allele

apodeme | embryonic stage

Cg25C^del.hs

common oviduct circular muscle | heat sensitive

Cg25C^DTS-L3

embryonic/larval brain

Cg25C^del.hs

embryonic/larval central nervous system

Cg25C^del.hs

embryonic/larval cuticle | dorsal

Cg25C^del.hs

embryonic/larval optic lobe

Cg25C^del.hs

embryonic/larval somatic muscle

Cg25C^del.hs

embryonic/larval visceral muscle

Cg25C^del.hs

head

Cg25C^del.hs

intracellular membrane-bounded organelle

Cg25C^DTS-L11

lateral oviduct circular muscle | heat sensitive

Cg25C^DTS-L3

leg & adult apodeme

Cg25C^del.hs

mitochondrial membrane

Cg25C^DTS-L11

muscle attachment site | embryonic stage

Cg25C^del.hs

ovarian basement membrane | heat sensitive

Cg25C^DTS-L3

somatic muscle | larval stage

Cg25C^del.hs

Classical Alleles ( 40 )

For All Classical Alleles Show

Allele of Cg25C	Mutagen	Stocks	Known lesion
Cg25C^A109.1F2	P-element activity	3	Yes
Cg25C^k00405	P-element activity	2	--
Cg25C^DTS-L2		1	--
Cg25C^EY15460	P-element activity	1	--
Cg25C^k03009	P-element activity	1	--
Cg25C^k03102	P-element activity	1	--
Cg25C^k09824	P-element activity	1	--
Cg25C^k13420	P-element activity	1	--
Cg25C^k16812	P-element activity	1	--
Cg25C^04518a	P-element activity	0	--
Cg25C²³⁴		0	--
Cg25C⁴¹¹		0	--
Cg25C⁴¹²		0	--
Cg25C⁴⁴		0	--
Cg25C^a-30		0	--
Cg25C^b-17		0	--
Cg25C^b-29		0	--
Cg25C^b-32		0	--
Cg25C^b-9		0	--
Cg25C^DCS-L11		0	--
Cg25C^DCS-L16		0	--
Cg25C^DCS-L20		0	--
Cg25C^DCS-L23		0	--
Cg25C^DTS-L10		0	--
Cg25C^DTS-L11		0	--
Cg25C^DTS-L12		0	--
Cg25C^DTS-L13		0	--
Cg25C^DTS-L14		0	--
Cg25C^DTS-L16		0	--
Cg25C^DTS-L3		0	Yes
Cg25C^DTS-L4	ethyl methanesulfonate	0	--
Cg25C^DTS-L5		0	--
Cg25C^h-13		0	--
Cg25C^h-30		0	--
Cg25C^hsneo10		0	--
Cg25C^hsneo119		0	--
Cg25C^ry-17		0	--
Cg25C^ry-1		0	--
Cg25C^ry-70		0	--
Cg25C^sz-74		0	--

Alleles Carried on Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Cg25C	Mutagen	Stocks	Known lesion
Cg25C^GD12784	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Cg25C^KK109933	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Cg25C^a.hs	in vitro construct - regulatory fusion	0	Yes
Cg25C^col.hs	in vitro construct - regulatory fusion	0	Yes
Cg25C^del.hs	in vitro construct - deletion	0	Yes
Cg25C^hs.9.7	in vitro construct - regulatory fusion	0	Yes
Cg25C^hs.PB	in vitro construct - regulatory fusion	0	Yes
Cg25C^{T:Disc\RFP-DsRed2}	gene targeting by homologous recombination SCEI endonuclease gene conversion	0	Yes
Cg25C^{TARG.T:Disc\RFP-DsRed2}	in vitro construct - genomic fragment in vitro construct - coding region fusion	0	Yes