FB2021_01, released Feb 18, 2021

Gene: Dmel\Cp16

Open Close
General Information
Symbol
Dmel\Cp16
Species
D. melanogaster
Name
Chorion protein 16
Annotation Symbol
CG6533
Feature Type
protein_coding_gene
FlyBase ID
FBgn0000356
Gene Model Status
Current
Stock Availability
4 publicly available
Gene Snapshot
Contributions welcome
Other Summaries
Also Known As

s16

Key Links
Genomic Location
Cytogenetic map
66D12-66D12
Sequence location
3L:8,731,944..8,732,599 [+]
Recombination map

3-26

RefSeq locus
NT_037436 REGION:8731944..8732599
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (3 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in dorsal appendage formation
inferred from mutant phenotype
(Peters et al., 2013)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in multicellular organism development
inferred from electronic annotation with InterPro:IPR008450
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
located_in egg chorion
inferred from electronic annotation with InterPro:IPR008450
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the chorion protein S16 family. (P22977)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
CHORION PROTEINS -
Chorion proteins (Cp) are encoded by gene clusters on the X and 3rd chromosome that undergo selective gene application in ovarian follicle cells during oogenesis. The Cp genes encode the major structural components of the eggshell chorion. (Adapted from FBrf0046094, FBrf0053248 and FBrf0227210.)
Protein Function (UniProtKB)
Chorion membrane (egg shell) protein; plays a role in protecting the egg from the environment.
(UniProt, P22977)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Cp16
The fourth of four chorion-protein genes in a 6-kb sequence; encodes S16-1, a 16,000-dalton chorion protein. Temporal and spatial distribution of expression described by Park and Spradling (1987, Genes Dev. 1: 497-509).
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Cp16 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Cp16-RA
FBtr0076574
NM_079257
522
138
Additional Transcript Data and Comments
Reported size (kB)

0.65 (northern blot)

(Griffin-Shea et al., 1982)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Cp16-PA
FBpp0076301
14.3
138
8.00
NP_523981
AAF50372
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cp16 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
oogenesis stage S11 -- S12 & adult stage | female
main body follicle cell | anterior | dorsal
(Niepielko et al., 2014)
oogenesis stage S12 & adult stage | female
main body follicle cell | anterior | ventral
(Niepielko et al., 2014)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
oogenesis stage S11 -- S14
(Griffin-Shea et al., 1982)
adult stage | female
(Griffin-Shea et al., 1982)
radioisotope in situ
Stage
Tissue/Position (including subcellular localization)
Reference
adult stageoogenesis stage S13 -- S14
follicle cell
(Parks and Spradling, 1987)
Additional Descriptive Data

Cp16 is expressed in all follicle cells, except for those associated with nurse cell remnants, and those at the tip of the micropyle.

(Parks and Spradling, 1987)

Cp16 transcript first appears in stage S11 of oogenesis, and expression peaks at stage S13-S14.

(Griffin-Shea et al., 1982)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | female
ovary
(Komitopoulou et al., 1988)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
Additional Descriptive Data

The six major chorion bands are dramatically reduced in follicles of Mcm6K1214, mus101K451 and fs(1)A1059K1 mutants. No reduction of major chorion bands is seen in swa6 although some affects are seen in higher molecular weight components.

(Komitopoulou et al., 1988)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Cp16 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 0 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Cp16
Transgenic constructs containing regulatory region of Cp16
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation
Inferred from location
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
dorsal appendage, with Scer\GAL4CY2
dorsal appendage, with Scer\GAL4Vm26Aa.F
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (0)
No records found.
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (0)
No records found.
Rattus norvegicus (Norway rat) (0)
No records found.
Xenopus tropicalis (Western clawed frog) (0)
No records found.
Danio rerio (Zebrafish) (0)
No records found.
Caenorhabditis elegans (Nematode, roundworm) (0)
No records found.
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190JLX )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00003771
Drosophila simulans
Dsim\Cp16
Drosophila sechellia
Dsec\GM25088
Drosophila erecta
Dere\GG14345
Drosophila yakuba
Dyak\Cp16
Drosophila ananassae
Dana\GF24350
Drosophila pseudoobscura pseudoobscura
Dpse\GA19666
Drosophila persimilis
Dper\GL10338
Drosophila willistoni
Dwil\GK16736
Drosophila virilis
Dvir\Cp16
Drosophila mojavensis
Dmoj\GI12926
Drosophila grimshawi
Dgri\Cp16
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150I1V )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17011032
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_09132
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Interactions Browser
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement
    View in Interactions Browser
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Cp16
    enhanceable
    ttk
    (Peters et al., 2013)
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3L
    Recombination map

    3-26

    Cytogenetic map
    66D12-66D12
    Sequence location
    3L:8,731,944..8,732,599 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    66D12-66D12
    Limits computationally determined from genome sequence between P{PZ}SrpRβrK561&P{lacW}l(3)j5B6j5B6 and P{PZ}l(3)0162901629&P{PZ}mRpL1210534
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    66D11-66D15
    (determined by in situ hybridisation)
    (Griffin-Shea et al., 1982)
    Experimentally Determined Recombination Data
    Location

    3-26

    (FlyBase, 2016)

    3-20.9

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (4)
    Genomic Clones (18)
    cDNA Clones (11)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of
        Source for database merge of
        Additional comments
        Other Comments

        Primarily regulated at the transcriptional level.

        (Romano et al., 1991)

        Two dimensional gels have been used to map the replication sites within the third chromosome chorion domain during amplification.

        (Heck and Spradling, 1990)

        5' flanking sequence conservation of Cp16 is very strong between species and extends further upstream than that for Cp15, Cp18 or Cp19.

        (Swimmer et al., 1990)

        The Cp15, Cp16, Cp18 and Cp19 genes of the chorion cluster have been characterized in D.melanogaster, D.subobscura, D.virilis and D.grimshawi. The temporally specific follicular expression of Cp15, Cp16, Cp18 and Cp19 and gene organization has been conserved. A highly conserved DNA region exists downstream of Cp16 and in the 5' flanking DNA.

        (Fenerjian et al., 1989)

        mus101K451, Mcm6K1214 and fs(1)A1059K1 mutants interfere with the amplification of the major chorion genes Cp15, Cp16, Cp18, Cp19, Cp36 and Cp38.

        (Komitopoulou et al., 1988)

        The fourth of four chorion-protein genes in a 6-kb sequence; encodes S16-1, a 16,000-dalton chorion protein. Temporal and spatial distribution of expression described (Parks and Spradling, 1987).

        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 24 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        Other crossreferences
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        Synonyms and Secondary IDs (14)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (80)