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Gene Dmel\Cp16

General Information
Symbol	Dmel\Cp16	Species	D. melanogaster
Name	Chorion protein 16	Annotation symbol	CG6533
Feature type	protein_coding_gene	FlyBase ID	FBgn0000356
Gene Model Status	Current	Stock availability	3 publicly available
Also Known As	s16
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	66D12-66D12	Sequence location	3L:8,725,044..8,725,699 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Chorion protein 16 is referred to in FlyBase by the symbol Dmel\Cp16 (CG6533, FBgn0000356). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: structural constituent of chorion. It is reported to be involved in the biological process: eggshell chorion assembly. 4 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Chorion S16. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of no expression detected. Peak expression observed in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1629380_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Cp16. Gene sequence location is 3L:8725044..8725699.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Cp16 The fourth of four chorion-protein genes in a 6-kb sequence; encodes S16-1, a 16,000-dalton chorion protein. Temporal and spatial distribution of expression described by Park and Spradling (1987, Genes Dev. 1: 497-509).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 66D12-66D12   Limits computationally determined from genome sequence between P{PZ}SrpRβrK561&P{lacW}l(3)j5B6j5B6 and P{PZ}l(3)0162901629&P{PZ}mRpL1210534
Experimentally Determined Cytological Location
Cytogenetic map Notes References 66D11-66D15   (determined by in situ hybridisation)   (Griffin-Shea et al., 1982)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Cp16 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.46
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Cp16-RA FBtr0076574  NM_079257   522   138
Additional Transcript Data & Comments
Reported size (kB)	0.65 (northern blot) (Griffin-Shea et al., 1982)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Cp16-PA   FBpp0076301   14.3   138   8.00     NP_523981   AAF50372
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Chorion S16 (IPR008450)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014296 AAF50372   BT030865   BT030872   BT030887   BT030893   BT032911   DQ062774 AAY56647   EV575709   EV582320   EV582655   EV586667   EV595218   EV600449   X16715 CAA34686
UniProtKB/Swiss-Prot	P22977
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference adult stage | female     (Griffin-Shea et al., 1982) oogenesis stage S11 -- S14     (Griffin-Shea et al., 1982) radioisotope in situ Stage Tissue/Position (including subcellular localization) Reference adult stageoogenesis stage S13 -- S14   follicle cell   (Parks and Spradling, 1987)
Additional Descriptive Data
	Cp16 is expressed in all follicle cells, except for those associated with nurse cell remnants, and those at the tip of the micropyle. (Parks and Spradling, 1987) Cp16 transcript first appears in stage S11 of oogenesis, and expression peaks at stage S13-S14. (Griffin-Shea et al., 1982)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference adult stage | female   ovary   (Komitopoulou et al., 1988) mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
	The six major chorion bands are dramatically reduced in follicles of Mcm6[K1214], mus101K451 and fs(1)A1059K1 mutants. No reduction of major chorion bands is seen in swa6 although some affects are seen in higher molecular weight components. (Komitopoulou et al., 1988)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    0.95 Larval Midgut    5 Larval Hindgut    1 Larval Malpighian Tubules    1.5 Larval Fat Body    2.3 Larval Salivary Gland    1.5 Larval Trachea    3.575 Larval Carcass    1.675 Adult Head    1.1 Adult Eye    3.225 Adult Brain    0.9 Adult Thoracic-Abdominal Ganglion    1.5 Adult Crop    1.3 Adult Midgut    1.9 Adult Hindgut    1.3 Adult Malpighian Tubules    1.4 Adult Fat Body    16.7 Adult Salivary Gland    4.3 Adult Heart    2.75 Adult VirginFemale Spermatheca    5.7 Adult InseminatedFemale Spermatheca    0.9 Adult Ovary    2891.5 Adult Testis    2.3 Adult Male Accessory Gland    2.5 Adult Carcass    29.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    0.95 Larval Midgut    5 Larval Hindgut    1 Larval Malpighian Tubules    1.5 Larval Fat Body    2.3 Larval Salivary Gland    1.5 Larval Trachea    3.575 Larval Carcass    1.675 Adult Head    1.1 Adult Eye    3.225 Adult Brain    0.9 Adult Thoracic-Abdominal Ganglion    1.5 Adult Crop    1.3 Adult Midgut    1.9 Adult Hindgut    1.3 Adult Malpighian Tubules    1.4 Adult Fat Body    16.7 Adult Salivary Gland    4.3 Adult Heart    2.75 Adult VirginFemale Spermatheca    5.7 Adult InseminatedFemale Spermatheca    0.9 Adult Ovary  (2891.5) Adult Testis    2.3 Adult Male Accessory Gland    2.5 Adult Carcass    29.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    0.95 Larval Midgut    5 Larval Hindgut    1 Larval Malpighian Tubules    1.5 Larval Fat Body    2.3 Larval Salivary Gland    1.5 Larval Trachea    3.575 Larval Carcass    1.675 Adult Head    1.1 Adult Eye    3.225 Adult Brain    0.9 Adult Thoracic-Abdominal Ganglion    1.5 Adult Crop    1.3 Adult Midgut    1.9 Adult Hindgut    1.3 Adult Malpighian Tubules    1.4 Adult Fat Body    16.7 Adult Salivary Gland    4.3 Adult Heart    2.75 Adult VirginFemale Spermatheca    5.7 Adult InseminatedFemale Spermatheca    0.9 Adult Ovary  (2891.5) Adult Testis    2.3 Adult Male Accessory Gland    2.5 Adult Carcass    29.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    0.95 Larval Midgut    5 Larval Hindgut    1 Larval Malpighian Tubules    1.5 Larval Fat Body    2.3 Larval Salivary Gland    1.5 Larval Trachea    3.575 Larval Carcass    1.675 Adult Head    1.1 Adult Eye    3.225 Adult Brain    0.9 Adult Thoracic-Abdominal Ganglion    1.5 Adult Crop    1.3 Adult Midgut    1.9 Adult Hindgut    1.3 Adult Malpighian Tubules    1.4 Adult Fat Body    16.7 Adult Salivary Gland    4.3 Adult Heart    2.75 Adult VirginFemale Spermatheca    5.7 Adult InseminatedFemale Spermatheca    0.9 Adult Ovary    2891.5 Adult Testis    2.3 Adult Male Accessory Gland    2.5 Adult Carcass    29.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    0.95 Larval Midgut    5 Larval Hindgut    1 Larval Malpighian Tubules    1.5 Larval Fat Body    2.3 Larval Salivary Gland    1.5 Larval Trachea    3.575 Larval Carcass    1.675 Adult Head    1.1 Adult Eye    3.225 Adult Brain    0.9 Adult Thoracic-Abdominal Ganglion    1.5 Adult Crop    1.3 Adult Midgut    1.9 Adult Hindgut    1.3 Adult Malpighian Tubules    1.4 Adult Fat Body    16.7 Adult Salivary Gland    4.3 Adult Heart    2.75 Adult VirginFemale Spermatheca    5.7 Adult InseminatedFemale Spermatheca    0.9 Adult Ovary    2891.5 Adult Testis    2.3 Adult Male Accessory Gland    2.5 Adult Carcass    29.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    0.95 Larval Midgut    5 Larval Hindgut    1 Larval Malpighian Tubules    1.5 Larval Fat Body    2.3 Larval Salivary Gland    1.5 Larval Trachea    3.575 Larval Carcass    1.675 Adult Head    1.1 Adult Eye    3.225 Adult Brain    0.9 Adult Thoracic-Abdominal Ganglion    1.5 Adult Crop    1.3 Adult Midgut    1.9 Adult Hindgut    1.3 Adult Malpighian Tubules    1.4 Adult Fat Body    16.7 Adult Salivary Gland    4.3 Adult Heart    2.75 Adult VirginFemale Spermatheca    5.7 Adult InseminatedFemale Spermatheca    0.9 Adult Ovary  (2891.5) Adult Testis    2.3 Adult Male Accessory Gland    2.5 Adult Carcass    29.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    0.95 Larval Midgut    5 Larval Hindgut    1 Larval Malpighian Tubules    1.5 Larval Fat Body    2.3 Larval Salivary Gland    1.5 Larval Trachea    3.575 Larval Carcass    1.675 Adult Head    1.1 Adult Eye    3.225 Adult Brain    0.9 Adult Thoracic-Abdominal Ganglion    1.5 Adult Crop    1.3 Adult Midgut    1.9 Adult Hindgut    1.3 Adult Malpighian Tubules    1.4 Adult Fat Body    16.7 Adult Salivary Gland    4.3 Adult Heart    2.75 Adult VirginFemale Spermatheca    5.7 Adult InseminatedFemale Spermatheca    0.9 Adult Ovary  (2891.5) Adult Testis    2.3 Adult Male Accessory Gland    2.5 Adult Carcass    29.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    0.95 Larval Midgut    5 Larval Hindgut    1 Larval Malpighian Tubules    1.5 Larval Fat Body    2.3 Larval Salivary Gland    1.5 Larval Trachea    3.575 Larval Carcass    1.675 Adult Head    1.1 Adult Eye    3.225 Adult Brain    0.9 Adult Thoracic-Abdominal Ganglion    1.5 Adult Crop    1.3 Adult Midgut    1.9 Adult Hindgut    1.3 Adult Malpighian Tubules    1.4 Adult Fat Body    16.7 Adult Salivary Gland    4.3 Adult Heart    2.75 Adult VirginFemale Spermatheca    5.7 Adult InseminatedFemale Spermatheca    0.9 Adult Ovary    2891.5 Adult Testis    2.3 Adult Male Accessory Gland    2.5 Adult Carcass    29.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    1.1   NA  Eye    3.225   NA  Brain    0.9   0.95  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1.5   NA  Crop    1.3   5  Midgut    1.9   1  Hindgut    1.3   1.5  Malpighian Tubules    1.4   2.3  Fat Body    no informative data   1.5  Salivary Gland    4.3   NA  Heart    2.75   3.575  Trachea    NA   NA  VirginFemale Spermatheca    5.7   NA  InseminatedFemale Spermatheca    0.9   NA  Ovary    2891.5   NA  Testis    2.3   NA  Male Accessory Gland    2.5   1.675  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    10 digestive system, 4-day adult    7 digestive system, 20-day adult    16 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    12 carcass, 4-day adult    51 carcass, 20-day adult    19 ovary, virgin 4-day female    47 ovary, mated 4-day female    107 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    10 digestive system, 4-day adult    7 digestive system, 20-day adult    16 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    12 carcass, 4-day adult  (51) carcass, 20-day adult    19 ovary, virgin 4-day female  (47) ovary, mated 4-day female  (107) testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    10 digestive system, 4-day adult    7 digestive system, 20-day adult    16 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    12 carcass, 4-day adult    51 carcass, 20-day adult    19 ovary, virgin 4-day female    47 ovary, mated 4-day female  107 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    10 digestive system, 4-day adult    7 digestive system, 20-day adult    16 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    12 carcass, 4-day adult    51 carcass, 20-day adult    19 ovary, virgin 4-day female    47 ovary, mated 4-day female    107 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    10 digestive system, 4-day adult    7 digestive system, 20-day adult    16 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    12 carcass, 4-day adult    51 carcass, 20-day adult    19 ovary, virgin 4-day female    47 ovary, mated 4-day female    107 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    10 digestive system, 4-day adult    7 digestive system, 20-day adult    16 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    12 carcass, 4-day adult  (51) carcass, 20-day adult    19 ovary, virgin 4-day female  (47) ovary, mated 4-day female  (107) testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    10 digestive system, 4-day adult    7 digestive system, 20-day adult    16 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    12 carcass, 4-day adult    51 carcass, 20-day adult    19 ovary, virgin 4-day female    47 ovary, mated 4-day female  107 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    10 digestive system, 4-day adult    7 digestive system, 20-day adult    16 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    12 carcass, 4-day adult    51 carcass, 20-day adult    19 ovary, virgin 4-day female    47 ovary, mated 4-day female    107 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of no expression detected. Peak expression observed in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    457 adult female 05day    1246 adult female 30day    1376 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day  (457) adult female 05day  (1246) adult female 30day  (1376) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day  (457) adult female 05day  (1246) adult female 30day  (1376) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    457 adult female 05day    1246 adult female 30day    1376 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    457 adult female 05day    1246 adult female 30day    1376 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day  (457) adult female 05day  (1246) adult female 30day  (1376) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day  (457) adult female 05day  (1246) adult female 30day  (1376) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    457 adult female 05day    1246 adult female 30day    1376 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    74 cold shock, 4-day adult    81 heat shock, 4-day adult    187 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    95 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    54 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (74) cold shock, 4-day adult  (81) heat shock, 4-day adult  (187) Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3  (95) Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult  (54) Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    74 cold shock, 4-day adult    81 heat shock, 4-day adult  (187) Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    95 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    54 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    74 cold shock, 4-day adult    81 heat shock, 4-day adult    187 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    95 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    54 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    74 cold shock, 4-day adult    81 heat shock, 4-day adult    187 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    95 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    54 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (74) cold shock, 4-day adult  (81) heat shock, 4-day adult  (187) Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3  (95) Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult  (54) Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    74 cold shock, 4-day adult    81 heat shock, 4-day adult  (187) Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    95 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    54 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    74 cold shock, 4-day adult    81 heat shock, 4-day adult    187 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    95 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    54 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000356 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6533-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 Cp16GD5021 Phenotype manifest in Allele
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of Cp16 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Cp16 Class Mutagen Stocks Known lesion Cp16GD5021 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Cp16KK112398 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Cp16NIG.6533R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Cp16R3.9 in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD5021} NA P{KK112398} NA P{NIG.6533R} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
structural constituent of chorion non-traceable author statement (FlyBase, 1992-)
Biological Process
CV term Evidence References
eggshell chorion assembly non-traceable author statement (FlyBase, 1992-) multicellular organismal development inferred from electronic annotation with InterPro:IPR008450 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
chorion inferred from electronic annotation with InterPro:IPR008450 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Cp16 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 64407 DroID - A comprehensive database of gene and protein interactions. • FBgn0000356 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39001
Orthologs
OrthoDB Orthologs (12) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG67PXSP Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG67PXSP)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Cp16     Drosophila simulans   Dsim\Cp16 Y   Drosophila sechellia   Dsec\GM25088 Y   Drosophila erecta   Dere\GG14345 Y   Drosophila yakuba   Dyak\Cp16 Y   Drosophila ananassae   Dana\GF24350 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA19666 Y   Drosophila persimilis   Dper\GL10338 Y   Drosophila willistoni   Dwil\GK16736 Y   Drosophila virilis   Dvir\Cp16 Y   Drosophila mojavensis   Dmoj\GI12926 Y   Drosophila grimshawi   Dgri\Cp16 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\Cp16 Drosophila sechellia Dsec\GM25088 Drosophila erecta Dere\GG14345 Drosophila yakuba Dyak\Cp16 Drosophila ananassae Dana\GF24350 Drosophila pseudoobscura pseudoobscura Dpse\GA19666 Drosophila persimilis Dper\GL10338 Drosophila willistoni Dwil\GK16736 Drosophila virilis Dvir\Cp16 Drosophila mojavensis Dmoj\GI12926 Drosophila grimshawi Dgri\Cp16
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
VDRC	v104999 P{KK112398}VIE-260B v19870 w1118; P{GD5021}v19870 v19872 w1118; P{GD5021}v19872/CyO
Genomic Clones ( 1 )
	BACR26I03 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 11 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones	IP19070 IP19164 IP19470 IP19564 IP19670
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	Dmel_dig_012744_0643_1483 Dmel_dig_014597_0631_2597 Dmel_dig_020594_0645_2355 Dmel_neb_002823_0696_0388 Dmel_neb_013381_0168_2457 Dmel_neb_016051_0957_0860
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0000356 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39001
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Primarily regulated at the transcriptional level. (Romano et al., 1991) Two dimensional gels have been used to map the replication sites within the third chromosome chorion domain during amplification. (Heck and Spradling, 1990) 5' flanking sequence conservation of Cp16 is very strong between species and extends further upstream than that for Cp15, Cp18 or Cp19. (Swimmer et al., 1990) The Cp15, Cp16, Cp18 and Cp19 genes of the chorion cluster have been characterized in D.melanogaster, D.subobscura, D.virilis and D.grimshawi. The temporally specific follicular expression of Cp15, Cp16, Cp18 and Cp19 and gene organization has been conserved. A highly conserved DNA region exists downstream of Cp16 and in the 5' flanking DNA. (Fenerjian et al., 1989) mus101K451, Mcm6K1214 and fs(1)A1059K1 mutants interfere with the amplification of the major chorion genes Cp15, Cp16, Cp18, Cp19, Cp36 and Cp38. (Komitopoulou et al., 1988) The fourth of four chorion-protein genes in a 6-kb sequence; encodes S16-1, a 16,000-dalton chorion protein. Temporal and spatial distribution of expression described (Parks and Spradling, 1987).
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079257 RefSeq (Proteins) • NP_523981 DDBJ / EMBL / GenBank • AAF50372 AAY56647 CAA34686 DQ062774 X16715 Entrez Gene - A searchable database of RefSeq genes. • 39001 UniProtKB/Swiss-Prot • P22977
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Chorion S16 (IPR008450)
Linkouts
	BioGRID - A database of protein and genetic interactions • 64407 DroID - A comprehensive database of gene and protein interactions. • FBgn0000356 DRSC - Results from RNAi screens. • FBgn0000356 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000356 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6533-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0000356 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39001 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39001 modMine - Data generated by the modENCODE project. • FBgn0000356
Synonyms & Secondary IDs ( 14 )
Reported As
Symbol Synonym	CG6533 (Fakhouri et al., 2006, Jagadeeshan and Singh, 2005, Keleman et al., 2009.8.5, Cammarato et al., 2011) Cp16 (Baker and Russell, 2009, Reiter et al., 2007, Domazet-Loso and Tautz, 2003, Hackney et al., 2007, Haerty et al., 2007, Lai et al., 2007, Japanese National Institute of Genetics, 2012.5.21) CP16 (Pletcher et al., 2002) cp16 (McVean and Vieira, 1999, Jagadeeshan and Singh, 2005, Calvi et al., 2007, Kim et al., 2011, Jagadeeshan and Singh, 2007) Dms16 (Vlachou and Komitopoulou, 2001) melcp16 (Jagadeeshan and Singh, 2007) Ov31 (Jagadeeshan and Singh, 2005) s16 (Claycomb and Orr-Weaver, 2005, Lu et al., 2001, Romano et al., 1991, Orr-Weaver, 1991, Swimmer et al., 1990, Heck and Spradling, 1990, Fenerjian et al., 1989, Cavaliere et al., 2008) S16   s16-1 (de Cicco and Spradling, 1984) X16715 (Ptak and Petrov, 2002)
Name Synonym	Chorion prot 16 (Carpenter et al., 2009) Chorion protein 16 (Fakhouri et al., 2006, Cammarato et al., 2011) Chorion Protein 16 (Dopman and Hartl, 2007)
Secondary FlyBase IDs
References ( 55 )
Generate a list of	All references relating to this gene ( 55 )
List References by type	Research paper  ( 38 ) Supplementary material  ( 3 ) Review  ( 2 ) Personal communication to FlyBase  ( 4 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Curated genome annotation  ( 2 ) Book  ( 1 ) DNA/RNA sequence record  ( 1 ) Protein sequence record  ( 1 )
Recent research papers ( 2 )
	Liu et al., 2012, Mol. Biol. Cell 23(1): 200--212 Analysis of model replication origins in Drosophila reveals new aspects of the chromatin landscape and its relationship to origin activity and the prereplicative complex. [FBrf0217098] Kim et al., 2011, Genes Dev. 25(13): 1384--1398 Integrative analysis of gene amplification in Drosophila follicle cells: parameters of origin activation and repression. [FBrf0214067] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...