FlyBase Gene Report: Dmel\Cp38

General Information

Symbol

Dmel\Cp38

Species

D. melanogaster

Name

Chorion protein 38

Annotation Symbol

CG11213

Feature Type

protein_coding_gene

FlyBase ID

FBgn0000360

Gene Model Status

Current

Stock Availability

2 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Red Book
Interactive Fly

Also Known As

s38, s38-1

Key Links

Genomic Location

Cytogenetic map

7F1-7F1

Sequence location

X:8,481,837..8,483,311 [+]

Recombination map

1-23

RefSeq locus

NC_004354 REGION:8481837..8483311

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (3 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables structural constituent of egg chorion

inferred from mutant phenotype

(Velentzas et al., 2018)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables structural constituent of egg chorion

inferred by curator from GO:0007304

(Niepielko et al., 2014)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in chorion-containing eggshell formation

inferred from mutant phenotype

(Velentzas et al., 2018)

inferred from expression pattern

(Niepielko et al., 2014)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in egg chorion

inferred from mutant phenotype

(Velentzas et al., 2018)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in egg chorion

inferred by curator from GO:0007304

(Niepielko et al., 2014)

Gene Group (FlyBase)

CHORION PROTEINS

Protein Family (UniProt)

Protein Signatures (InterPro)

Chorion S36/S38

Summaries

Gene Group (FlyBase)

CHORION PROTEINS -: Chorion proteins (Cp) are encoded by gene clusters on the X and 3rd chromosome that undergo selective gene application in ovarian follicle cells during oogenesis. The Cp genes encode the major structural components of the eggshell chorion. (Adapted from FBrf0046094, FBrf0053248 and FBrf0227210.)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Cp38

Structural gene for s38-1, a 38,000-dalton chorion protein. Synthesis primarily during stages 12 and 13 (Waring and Mahowald, 1979, Cell 16: 599-607) and during stages 11 and 12 (Petri, Wyman, and Kafatos, 1976, Dev. Biol. 49: 185-199). 2,000 to 3,000 daltons cleaved from primary translation product to yield mature protein (Spradling et al., 1980). Temporal and spatial distribution of expression described by Park and Spradling (1987, Genes Dev. 1: 497-509).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Cp38 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P07183)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Cp38-RA

FBtr0071204

NM_078533

1112

306

Cp38-RB

FBtr0300448

NM_001169232

1249

306

Additional Transcript Data and Comments

Reported size (kB)

1.4 (northern blot)

(Parks et al., 1986)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Cp38-PA

FBpp0071151

30.4

306

9.43

FBpp0289676

30.4

306

9.43

P07183

NP_001162703

ACZ95238

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

306 aa isoforms: Cp38-PA, Cp38-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Chorion S36/S38

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P07183

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cp38 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.35

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis stage S10B -- S11 & adult stage | female

main body follicle cell | anterior | dorsal

(Niepielko et al., 2014)

floor cell

(Niepielko et al., 2014)

roof cell

(Niepielko et al., 2014)

oogenesis stage S11 & adult stage | female

main body follicle cell | anterior | ventral

(Niepielko et al., 2014)

main body follicle cell | posterior

(Niepielko et al., 2014)

main body follicle cell

Comment: minus midline cells

(Niepielko et al., 2014)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis stage S10 -- S14

(Parks et al., 1986)

radioisotope in situ

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage oogenesis stage S10B -- S14

follicle cell

(Parks and Spradling, 1987)

Additional Descriptive Data

Cp38 and Cp36 are initially expressed in a dorsal subpopulation of stage S10B follicle cells. Later, they are expressed in most follicle cells.

(Parks and Spradling, 1987)

Expression of the 1.4 kb Cp38 transcript peaks at stages S12-S13 of oogenesis.

(Parks et al., 1986)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

ovary

(Komitopoulou et al., 1988)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

The six major chorion bands are dramatically reduced in follicles of Mcm6^K1214, mus101^K451 and fs(1)A1059^K1 mutants. No reduction of major chorion bands is seen in swa⁶ although some affects are seen in higher molecular weight components.

(Komitopoulou et al., 1988)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in egg chorion

inferred from mutant phenotype

(Velentzas et al., 2018)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Cp38 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Cp38

View exonic expression by tissue for Dmel\Cp38

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31788/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0000360

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0000360

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0000360

Flygut - An atlas of the Drosophila adult midgut

FBgn0000360

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 29 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Cp38

Transgenic constructs containing regulatory region of Cp38

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(1)otu-PΔ1

(Sass et al., 1993)

Inferred from location ( 16 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Cp38^GD6312

viable, with Scer\GAL4^Mef2.PR

Cp38^GD6312

viable, with Scer\GAL4^pnr-MD237

Cp38^GD6312

Other Phenotypes

Allele

abnormal memory | adult stage, with Scer\GAL4^GMR13F02

Cp38^KK112005

decreased size | embryonic stage, with Scer\GAL4^c355

electrophoretic variant

increased mortality during development, with Scer\GAL4^c355

Phenotype manifest in

Allele

chorion, with Scer\GAL4^c355

Cp38^KK112005

dorsal appendage, with Scer\GAL4^c355

egg, with Scer\GAL4^c355

Cp38^KK112005

micropyle, with Scer\GAL4^c355

Cp38^KK112005

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (0)

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Cp38. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

58247

DroID - A comprehensive database of gene and protein interactions.

FBgn0000360

MIST (protein-protein) - An integrated Molecular Interaction Database

31788

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-23

Cytogenetic map

7F1-7F1

Sequence location

X:8,481,837..8,483,311 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

7F1-7F1

Limits computationally determined from genome sequence between P{EP}CG1632^EP1583 and P{EP}Moe^EP1652

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-23

(FlyBase, 2016)

1-23.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (2)

Aberrations including deletions of this gene Aberrations including duplications of this gene

VDRC

v106008

P{KK112005}VIE-260B

v41344

w¹¹¹⁸; P{GD6312}v41344

Genomic Clones (7)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (21)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0000360

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0000360

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Valina et al., 2024)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.

(Kliman and Eyre-Walker, 1998)

Primarily regulated at the transcriptional level.

(Romano et al., 1991)

The structure and functional features of Cp36 and Cp38 have been determined.

(Hamodrakas et al., 1989)

mus101^K451, Mcm6^K1214 and fs(1)A1059^K1 mutants interfere with the amplification of the major chorion genes Cp15, Cp16, Cp18, Cp19, Cp36 and Cp38.

(Komitopoulou et al., 1988)

Transcription termination sites of Cp36 and Cp38 have been mapped using EM analysis. Results demonstrate that Cp36 and Cp38 terminate more closely to the poly(A) addition sites and in a shorter region than many other polyadenylation genes examined to date.

(Osheim et al., 1986)

Tissue and stage specific expression of Cp15 and Cp38 are retained when the genes are removed from the remaining genes of the cluster, and in the absence of amplification. Deletion analysis has delimited sequences regulating Cp38 transcription to within a portion of its upstream spacer and 5' transcribed region.

(Wakimoto et al., 1986)

Structural gene for s38-1, a 38,000-dalton chorion protein. Synthesis primarily during stages 12 and 13 (Waring and Mahowald) and during stages 11 and 12 (Petri, Wyman and Kafatos, 1976). 2,000 to 3,000 daltons cleaved from primary translation product to yield mature protein (Spradling, Digan, Mahowald, Scott and Craig, 1980). Temporal and spatial distribution of expression described by Parks and Spradling (1987).

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

ACE1

CG11213

(Guven-Ozkan et al., 2016, Cammarato et al., 2011, Keleman et al., 2009.8.5, Bhutkar et al., 2008, Fakhouri et al., 2006)

CP38

(Pletcher et al., 2002)

Cp38

(Valina et al., 2024, Cridland et al., 2020, Ekhteraei-Tousi et al., 2020, Hua and Orr-Weaver, 2017, Alexander et al., 2016, Guven-Ozkan et al., 2016, Brown et al., 2014, Baker and Russell, 2009, Bhutkar et al., 2008, Hackney et al., 2007, Lai et al., 2007, Reiter et al., 2007, Parisi et al., 2004)

Dms38

(Vlachou and Komitopoulou, 2001)

(Fargnoli and Waring, 1984)

S38

(Spradling, 1997.11.8)

cp38

(Jagadeeshan and Singh, 2007)

s38

(Valina et al., 2024, Velentzas et al., 2018, Velentzas et al., 2016, Cavaliere et al., 2008, Fakhouri et al., 2006, Mazina et al., 1991, Orr-Weaver, 1991, Romano et al., 1991, Hamodrakas et al., 1989)

s38-1

(Osheim et al., 1986, Parks et al., 1986, Wakimoto et al., 1986, Wakimoto et al., 1986)

Name Synonyms

Chorion prot 38

(Carpenter et al., 2009)

Chorion protein 38

(Cammarato et al., 2011, McGraw et al., 2008, Fakhouri et al., 2006, Harbison et al., 2005)

Shell-38

Secondary FlyBase IDs

FBgn0004940

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 27 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31788

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

X05245

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P07183

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P07183

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.