Gene Dmel\crm

l(1)3Bf

(Rayle and Green, 1968)

Notes

Mapping based on crm^sa with w.

No crossing over with w in 254 flies.

(Bateman, 1951)

Gene Model & Products

Please see the GBrowse view of Dmel\crm for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

crm-RA

FBtr0070496

NM_057757

3780

982

crm-RB

FBtr0070495

NM_166951

3837

982

Additional Transcript Data & Comments

Reported size (kB)

3.793 (longest cDNA)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

crm-PA

FBpp0070473

107.4

982

8.23

NP_477105

AAG22402

crm-PB

FBpp0070472

107.4

982

8.23

NP_726829

AAF45822

Additional Polypeptide Data & Comments

Reported size (kDa)

693 (aa); 110 (kD observed); 75 (kD predicted)

Comments

External Data

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

SANT/Myb domain (IPR001005)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

O76906

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment:maternally deposited

embryonic stage 4 -- 6

organism

embryonic stage 7 -- 8

organism | ubiquitous

embryonic stage 9 -- 10

organism | ubiquitous

embryonic stage 11 -- 12

organism | ubiquitous

embryonic stage 13 -- 16

embryonic brain

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage | early

embryonic stage

presumptive embryonic/larval nervous system

gonad

pole cell

third instar larval stage

embryonic/larval salivary gland

Additional Descriptive Data

crm protein localization is cell-cycle dependent. crm protein localizes to the nucleus during S phase and disappears at mitosis.

Marker for

Subcellular Localization (GO Cellular Component)

CV term

References

nucleus

inferred from direct assay

(assigned by UniProtKB)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult ovary.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		110.025
Larval Midgut		47.7
Larval Hindgut		53
Larval Malpighian Tubules		34
Larval Fat Body		33.8
Larval Salivary Gland		57.4
Larval Trachea		50.125
Larval Carcass		51.275
Adult Head		61
Adult Eye		31.475
Adult Brain		112.1
Adult Thoracic-Abdominal Ganglion		89.2
Adult Crop		61.9
Adult Midgut		40
Adult Hindgut		52.6
Adult Malpighian Tubules		29.9
Adult Fat Body		47.8
Adult Salivary Gland		68.6
Adult Heart		14.125
Adult VirginFemale Spermatheca		29.3
Adult InseminatedFemale Spermatheca		32.3
Adult Ovary		350.8
Adult Testis		17.9
Adult Male Accessory Gland		62.8
Adult Carcass		64.6
Expression Level Scale		None Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		110.025
Larval Midgut		47.7
Larval Hindgut		53
Larval Malpighian Tubules		34
Larval Fat Body		33.8
Larval Salivary Gland		57.4
Larval Trachea		50.125
Larval Carcass		51.275
Adult Head		61
Adult Eye		31.475
Adult Brain		112.1
Adult Thoracic-Abdominal Ganglion		89.2
Adult Crop		61.9
Adult Midgut		40
Adult Hindgut		52.6
Adult Malpighian Tubules		29.9
Adult Fat Body		47.8
Adult Salivary Gland		68.6
Adult Heart		14.125
Adult VirginFemale Spermatheca		29.3
Adult InseminatedFemale Spermatheca		32.3
Adult Ovary		350.8
Adult Testis		17.9
Adult Male Accessory Gland		62.8
Adult Carcass		64.6
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		110.025
Larval Midgut		47.7
Larval Hindgut		53
Larval Malpighian Tubules		34
Larval Fat Body		33.8
Larval Salivary Gland		57.4
Larval Trachea		50.125
Larval Carcass		51.275
Adult Head		61
Adult Eye		31.475
Adult Brain		112.1
Adult Thoracic-Abdominal Ganglion		89.2
Adult Crop		61.9
Adult Midgut		40
Adult Hindgut		52.6
Adult Malpighian Tubules		29.9
Adult Fat Body		47.8
Adult Salivary Gland		68.6
Adult Heart		14.125
Adult VirginFemale Spermatheca		29.3
Adult InseminatedFemale Spermatheca		32.3
Adult Ovary		350.8
Adult Testis		17.9
Adult Male Accessory Gland		62.8
Adult Carcass		64.6
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		110.025
Larval Midgut		47.7
Larval Hindgut		53
Larval Malpighian Tubules		34
Larval Fat Body		33.8
Larval Salivary Gland		57.4
Larval Trachea		50.125
Larval Carcass		51.275
Adult Head		61
Adult Eye		31.475
Adult Brain		112.1
Adult Thoracic-Abdominal Ganglion		89.2
Adult Crop		61.9
Adult Midgut		40
Adult Hindgut		52.6
Adult Malpighian Tubules		29.9
Adult Fat Body		47.8
Adult Salivary Gland		68.6
Adult Heart		14.125
Adult VirginFemale Spermatheca		29.3
Adult InseminatedFemale Spermatheca		32.3
Adult Ovary		350.8
Adult Testis		17.9
Adult Male Accessory Gland		62.8
Adult Carcass		64.6
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		110.025
Larval Midgut		47.7
Larval Hindgut		53
Larval Malpighian Tubules		34
Larval Fat Body		33.8
Larval Salivary Gland		57.4
Larval Trachea		50.125
Larval Carcass		51.275
Adult Head		61
Adult Eye		31.475
Adult Brain		112.1
Adult Thoracic-Abdominal Ganglion		89.2
Adult Crop		61.9
Adult Midgut		40
Adult Hindgut		52.6
Adult Malpighian Tubules		29.9
Adult Fat Body		47.8
Adult Salivary Gland		68.6
Adult Heart		14.125
Adult VirginFemale Spermatheca		29.3
Adult InseminatedFemale Spermatheca		32.3
Adult Ovary		350.8
Adult Testis		17.9
Adult Male Accessory Gland		62.8
Adult Carcass		64.6
Expression Level Scale		None Low Moderate High

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		110.025
Larval Midgut		47.7
Larval Hindgut		53
Larval Malpighian Tubules		34
Larval Fat Body		33.8
Larval Salivary Gland		57.4
Larval Trachea		50.125
Larval Carcass		51.275
Adult Head		61
Adult Eye		31.475
Adult Brain		112.1
Adult Thoracic-Abdominal Ganglion		89.2
Adult Crop		61.9
Adult Midgut		40
Adult Hindgut		52.6
Adult Malpighian Tubules		29.9
Adult Fat Body		47.8
Adult Salivary Gland		68.6
Adult Heart		14.125
Adult VirginFemale Spermatheca		29.3
Adult InseminatedFemale Spermatheca		32.3
Adult Ovary		350.8
Adult Testis		17.9
Adult Male Accessory Gland		62.8
Adult Carcass		64.6
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		110.025
Larval Midgut		47.7
Larval Hindgut		53
Larval Malpighian Tubules		34
Larval Fat Body		33.8
Larval Salivary Gland		57.4
Larval Trachea		50.125
Larval Carcass		51.275
Adult Head		61
Adult Eye		31.475
Adult Brain		112.1
Adult Thoracic-Abdominal Ganglion		89.2
Adult Crop		61.9
Adult Midgut		40
Adult Hindgut		52.6
Adult Malpighian Tubules		29.9
Adult Fat Body		47.8
Adult Salivary Gland		68.6
Adult Heart		14.125
Adult VirginFemale Spermatheca		29.3
Adult InseminatedFemale Spermatheca		32.3
Adult Ovary		350.8
Adult Testis		17.9
Adult Male Accessory Gland		62.8
Adult Carcass		64.6
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		110.025
Larval Midgut		47.7
Larval Hindgut		53
Larval Malpighian Tubules		34
Larval Fat Body		33.8
Larval Salivary Gland		57.4
Larval Trachea		50.125
Larval Carcass		51.275
Adult Head		61
Adult Eye		31.475
Adult Brain		112.1
Adult Thoracic-Abdominal Ganglion		89.2
Adult Crop		61.9
Adult Midgut		40
Adult Hindgut		52.6
Adult Malpighian Tubules		29.9
Adult Fat Body		47.8
Adult Salivary Gland		68.6
Adult Heart		14.125
Adult VirginFemale Spermatheca		29.3
Adult InseminatedFemale Spermatheca		32.3
Adult Ovary		350.8
Adult Testis		17.9
Adult Male Accessory Gland		62.8
Adult Carcass		64.6
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	61
NA	Eye	31.475
NA	Brain	112.1
110.025	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	89.2
NA	Crop	61.9
47.7	Midgut	40
53	Hindgut	52.6
34	Malpighian Tubules	no informative data
33.8	Fat Body	47.8
57.4	Salivary Gland	68.6
NA	Heart	14.125
50.125	Trachea	NA
NA	VirginFemale Spermatheca	29.3
NA	InseminatedFemale Spermatheca	32.3
NA	Ovary	350.8
NA	Testis	no informative data
NA	Male Accessory Gland	62.8
51.275	Carcass	64.6

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		10
central nervous system, larvae L3		11
central nervous system, pupae P8		5
head, virgin 1-day female		3
head, virgin 4-day female		7
head, virgin 20-day female		6
head, mated 1-day female		4
head, mated 4-day female		4
head, mated 20-day female		6
head, mated 1-day male		7
head, mated 4-day male		7
head, mated 20-day male		10
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		4
digestive system, 20-day adult		4
fat body, larvae L3 wandering		1
fat body, white prepupae		2
fat body, pupae P8		2
carcass, larvae L3 wandering		6
carcass, 1-day adult		3
carcass, 4-day adult		5
carcass, 20-day adult		6
ovary, virgin 4-day female		64
ovary, mated 4-day female		65
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		10
central nervous system, larvae L3		11
central nervous system, pupae P8		5
head, virgin 1-day female		3
head, virgin 4-day female		7
head, virgin 20-day female		6
head, mated 1-day female		4
head, mated 4-day female		4
head, mated 20-day female		6
head, mated 1-day male		7
head, mated 4-day male		7
head, mated 20-day male		10
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		4
digestive system, 20-day adult		4
fat body, larvae L3 wandering		1
fat body, white prepupae		2
fat body, pupae P8		2
carcass, larvae L3 wandering		6
carcass, 1-day adult		3
carcass, 4-day adult		5
carcass, 20-day adult		6
ovary, virgin 4-day female		(64)
ovary, mated 4-day female		(65)
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		10
central nervous system, larvae L3		11
central nervous system, pupae P8		5
head, virgin 1-day female		3
head, virgin 4-day female		7
head, virgin 20-day female		6
head, mated 1-day female		4
head, mated 4-day female		4
head, mated 20-day female		6
head, mated 1-day male		7
head, mated 4-day male		7
head, mated 20-day male		10
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		4
digestive system, 20-day adult		4
fat body, larvae L3 wandering		1
fat body, white prepupae		2
fat body, pupae P8		2
carcass, larvae L3 wandering		6
carcass, 1-day adult		3
carcass, 4-day adult		5
carcass, 20-day adult		6
ovary, virgin 4-day female		64
ovary, mated 4-day female		65
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		10
central nervous system, larvae L3		11
central nervous system, pupae P8		5
head, virgin 1-day female		3
head, virgin 4-day female		7
head, virgin 20-day female		6
head, mated 1-day female		4
head, mated 4-day female		4
head, mated 20-day female		6
head, mated 1-day male		7
head, mated 4-day male		7
head, mated 20-day male		10
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		4
digestive system, 20-day adult		4
fat body, larvae L3 wandering		1
fat body, white prepupae		2
fat body, pupae P8		2
carcass, larvae L3 wandering		6
carcass, 1-day adult		3
carcass, 4-day adult		5
carcass, 20-day adult		6
ovary, virgin 4-day female		64
ovary, mated 4-day female		65
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		10
central nervous system, larvae L3		11
central nervous system, pupae P8		5
head, virgin 1-day female		3
head, virgin 4-day female		7
head, virgin 20-day female		6
head, mated 1-day female		4
head, mated 4-day female		4
head, mated 20-day female		6
head, mated 1-day male		7
head, mated 4-day male		7
head, mated 20-day male		10
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		4
digestive system, 20-day adult		4
fat body, larvae L3 wandering		1
fat body, white prepupae		2
fat body, pupae P8		2
carcass, larvae L3 wandering		6
carcass, 1-day adult		3
carcass, 4-day adult		5
carcass, 20-day adult		6
ovary, virgin 4-day female		64
ovary, mated 4-day female		65
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		10
central nervous system, larvae L3		11
central nervous system, pupae P8		5
head, virgin 1-day female		3
head, virgin 4-day female		7
head, virgin 20-day female		6
head, mated 1-day female		4
head, mated 4-day female		4
head, mated 20-day female		6
head, mated 1-day male		7
head, mated 4-day male		7
head, mated 20-day male		10
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		4
digestive system, 20-day adult		4
fat body, larvae L3 wandering		1
fat body, white prepupae		2
fat body, pupae P8		2
carcass, larvae L3 wandering		6
carcass, 1-day adult		3
carcass, 4-day adult		5
carcass, 20-day adult		6
ovary, virgin 4-day female		(64)
ovary, mated 4-day female		(65)
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		10
central nervous system, larvae L3		11
central nervous system, pupae P8		5
head, virgin 1-day female		3
head, virgin 4-day female		7
head, virgin 20-day female		6
head, mated 1-day female		4
head, mated 4-day female		4
head, mated 20-day female		6
head, mated 1-day male		7
head, mated 4-day male		7
head, mated 20-day male		10
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		4
digestive system, 20-day adult		4
fat body, larvae L3 wandering		1
fat body, white prepupae		2
fat body, pupae P8		2
carcass, larvae L3 wandering		6
carcass, 1-day adult		3
carcass, 4-day adult		5
carcass, 20-day adult		6
ovary, virgin 4-day female		64
ovary, mated 4-day female		65
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		10
central nervous system, larvae L3		11
central nervous system, pupae P8		5
head, virgin 1-day female		3
head, virgin 4-day female		7
head, virgin 20-day female		6
head, mated 1-day female		4
head, mated 4-day female		4
head, mated 20-day female		6
head, mated 1-day male		7
head, mated 4-day male		7
head, mated 20-day male		10
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		2
digestive system, 4-day adult		4
digestive system, 20-day adult		4
fat body, larvae L3 wandering		1
fat body, white prepupae		2
fat body, pupae P8		2
carcass, larvae L3 wandering		6
carcass, 1-day adult		3
carcass, 4-day adult		5
carcass, 20-day adult		6
ovary, virgin 4-day female		64
ovary, mated 4-day female		65
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		98
embryo 02-04hr		63
embryo 04-06hr		34
embryo 06-08hr		34
embryo 08-10hr		36
embryo 10-12hr		25
embryo 12-14hr		20
embryo 14-16hr		28
embryo 16-18hr		20
embryo 18-20hr		13
embryo 20-22hr		19
embryo 22-24hr		20
larva L1		6
larva L2		3
larva L3 12hr old		3
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		7
white prepupae new		6
white prepupae 12hr		8
white prepupae 24hr		10
pupae 2d postWPP		6
pupae 3d postWPP		3
pupae 4d postWPP		4
adult male 01day		4
adult male 05day		5
adult male 30day		6
adult female 01day		27
adult female 05day		48
adult female 30day		55
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(98)
embryo 02-04hr		(63)
embryo 04-06hr		(34)
embryo 06-08hr		(34)
embryo 08-10hr		(36)
embryo 10-12hr		25
embryo 12-14hr		20
embryo 14-16hr		(28)
embryo 16-18hr		20
embryo 18-20hr		13
embryo 20-22hr		19
embryo 22-24hr		20
larva L1		6
larva L2		3
larva L3 12hr old		3
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		7
white prepupae new		6
white prepupae 12hr		8
white prepupae 24hr		10
pupae 2d postWPP		6
pupae 3d postWPP		3
pupae 4d postWPP		4
adult male 01day		4
adult male 05day		5
adult male 30day		6
adult female 01day		(27)
adult female 05day		(48)
adult female 30day		(55)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		98
embryo 02-04hr		63
embryo 04-06hr		34
embryo 06-08hr		34
embryo 08-10hr		36
embryo 10-12hr		25
embryo 12-14hr		20
embryo 14-16hr		28
embryo 16-18hr		20
embryo 18-20hr		13
embryo 20-22hr		19
embryo 22-24hr		20
larva L1		6
larva L2		3
larva L3 12hr old		3
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		7
white prepupae new		6
white prepupae 12hr		8
white prepupae 24hr		10
pupae 2d postWPP		6
pupae 3d postWPP		3
pupae 4d postWPP		4
adult male 01day		4
adult male 05day		5
adult male 30day		6
adult female 01day		27
adult female 05day		48
adult female 30day		55
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		98
embryo 02-04hr		63
embryo 04-06hr		34
embryo 06-08hr		34
embryo 08-10hr		36
embryo 10-12hr		25
embryo 12-14hr		20
embryo 14-16hr		28
embryo 16-18hr		20
embryo 18-20hr		13
embryo 20-22hr		19
embryo 22-24hr		20
larva L1		6
larva L2		3
larva L3 12hr old		3
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		7
white prepupae new		6
white prepupae 12hr		8
white prepupae 24hr		10
pupae 2d postWPP		6
pupae 3d postWPP		3
pupae 4d postWPP		4
adult male 01day		4
adult male 05day		5
adult male 30day		6
adult female 01day		27
adult female 05day		48
adult female 30day		55
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		98
embryo 02-04hr		63
embryo 04-06hr		34
embryo 06-08hr		34
embryo 08-10hr		36
embryo 10-12hr		25
embryo 12-14hr		20
embryo 14-16hr		28
embryo 16-18hr		20
embryo 18-20hr		13
embryo 20-22hr		19
embryo 22-24hr		20
larva L1		6
larva L2		3
larva L3 12hr old		3
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		7
white prepupae new		6
white prepupae 12hr		8
white prepupae 24hr		10
pupae 2d postWPP		6
pupae 3d postWPP		3
pupae 4d postWPP		4
adult male 01day		4
adult male 05day		5
adult male 30day		6
adult female 01day		27
adult female 05day		48
adult female 30day		55
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(98)
embryo 02-04hr		(63)
embryo 04-06hr		(34)
embryo 06-08hr		(34)
embryo 08-10hr		(36)
embryo 10-12hr		25
embryo 12-14hr		20
embryo 14-16hr		28
embryo 16-18hr		20
embryo 18-20hr		13
embryo 20-22hr		19
embryo 22-24hr		20
larva L1		6
larva L2		3
larva L3 12hr old		3
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		7
white prepupae new		6
white prepupae 12hr		8
white prepupae 24hr		10
pupae 2d postWPP		6
pupae 3d postWPP		3
pupae 4d postWPP		4
adult male 01day		4
adult male 05day		5
adult male 30day		6
adult female 01day		27
adult female 05day		(48)
adult female 30day		(55)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		98
embryo 02-04hr		63
embryo 04-06hr		34
embryo 06-08hr		34
embryo 08-10hr		36
embryo 10-12hr		25
embryo 12-14hr		20
embryo 14-16hr		28
embryo 16-18hr		20
embryo 18-20hr		13
embryo 20-22hr		19
embryo 22-24hr		20
larva L1		6
larva L2		3
larva L3 12hr old		3
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		7
white prepupae new		6
white prepupae 12hr		8
white prepupae 24hr		10
pupae 2d postWPP		6
pupae 3d postWPP		3
pupae 4d postWPP		4
adult male 01day		4
adult male 05day		5
adult male 30day		6
adult female 01day		27
adult female 05day		48
adult female 30day		55
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		98
embryo 02-04hr		63
embryo 04-06hr		34
embryo 06-08hr		34
embryo 08-10hr		36
embryo 10-12hr		25
embryo 12-14hr		20
embryo 14-16hr		28
embryo 16-18hr		20
embryo 18-20hr		13
embryo 20-22hr		19
embryo 22-24hr		20
larva L1		6
larva L2		3
larva L3 12hr old		3
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		7
larva L3 puffstage 7-9		7
white prepupae new		6
white prepupae 12hr		8
white prepupae 24hr		10
pupae 2d postWPP		6
pupae 3d postWPP		3
pupae 4d postWPP		4
adult male 01day		4
adult male 05day		5
adult male 30day		6
adult female 01day		27
adult female 05day		48
adult female 30day		55
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		12
Schneider line 2 Sg4		7
embryonic 1182-4H		11
embryonic GM2		8
embryonic Kc167		8
embryonic S1		11
embryonic S3		6
leg disc CME L1		8
wing disc CME-W2		5
wing disc ML-DmD8		8
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		11
wing disc ML-DmD21		10
wing disc ML-DmD32		21
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		7
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		9
tumorous blood cells mbn2		7
ovary fGS/OSS		19
ovary OSC		7
ovary OSS		4
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		12
Schneider line 2 Sg4		7
embryonic 1182-4H		11
embryonic GM2		8
embryonic Kc167		8
embryonic S1		11
embryonic S3		6
leg disc CME L1		8
wing disc CME-W2		5
wing disc ML-DmD8		8
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		11
wing disc ML-DmD21		10
wing disc ML-DmD32		21
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		7
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		9
tumorous blood cells mbn2		7
ovary fGS/OSS		19
ovary OSC		7
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		12
Schneider line 2 Sg4		7
embryonic 1182-4H		11
embryonic GM2		8
embryonic Kc167		8
embryonic S1		11
embryonic S3		6
leg disc CME L1		8
wing disc CME-W2		5
wing disc ML-DmD8		8
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		11
wing disc ML-DmD21		10
wing disc ML-DmD32		21
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		7
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		9
tumorous blood cells mbn2		7
ovary fGS/OSS		19
ovary OSC		7
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		12
Schneider line 2 Sg4		7
embryonic 1182-4H		11
embryonic GM2		8
embryonic Kc167		8
embryonic S1		11
embryonic S3		6
leg disc CME L1		8
wing disc CME-W2		5
wing disc ML-DmD8		8
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		11
wing disc ML-DmD21		10
wing disc ML-DmD32		21
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		7
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		9
tumorous blood cells mbn2		7
ovary fGS/OSS		19
ovary OSC		7
ovary OSS		4
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		12
Schneider line 2 Sg4		7
embryonic 1182-4H		11
embryonic GM2		8
embryonic Kc167		8
embryonic S1		11
embryonic S3		6
leg disc CME L1		8
wing disc CME-W2		5
wing disc ML-DmD8		8
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		11
wing disc ML-DmD21		10
wing disc ML-DmD32		21
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		7
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		9
tumorous blood cells mbn2		7
ovary fGS/OSS		19
ovary OSC		7
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		12
Schneider line 2 Sg4		7
embryonic 1182-4H		11
embryonic GM2		8
embryonic Kc167		8
embryonic S1		11
embryonic S3		6
leg disc CME L1		8
wing disc CME-W2		5
wing disc ML-DmD8		8
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		11
wing disc ML-DmD21		10
wing disc ML-DmD32		21
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		7
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		9
tumorous blood cells mbn2		7
ovary fGS/OSS		19
ovary OSC		7
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		12
Schneider line 2 Sg4		7
embryonic 1182-4H		11
embryonic GM2		8
embryonic Kc167		8
embryonic S1		11
embryonic S3		6
leg disc CME L1		8
wing disc CME-W2		5
wing disc ML-DmD8		8
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		11
wing disc ML-DmD21		10
wing disc ML-DmD32		21
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		7
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		9
tumorous blood cells mbn2		7
ovary fGS/OSS		19
ovary OSC		7
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		12
Schneider line 2 Sg4		7
embryonic 1182-4H		11
embryonic GM2		8
embryonic Kc167		8
embryonic S1		11
embryonic S3		6
leg disc CME L1		8
wing disc CME-W2		5
wing disc ML-DmD8		8
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		11
wing disc ML-DmD21		10
wing disc ML-DmD32		21
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		7
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		11
CNS ML-DmBG2-c2		9
tumorous blood cells mbn2		7
ovary fGS/OSS		19
ovary OSC		7
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		12
cold shock, 4-day adult		6
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		47
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		37
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		40
Caffeine 25 mg/ml 48 hrs, 4-day adult		12
Paraquat 5 mM 48 hrs, 4-day adult		31
Paraquat 10 mM 48 hrs, 4-day adult		26
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		12
cold shock, 4-day adult		6
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		(47)
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		(37)
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(40)
Caffeine 25 mg/ml 48 hrs, 4-day adult		12
Paraquat 5 mM 48 hrs, 4-day adult		(31)
Paraquat 10 mM 48 hrs, 4-day adult		26
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		12
cold shock, 4-day adult		6
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		47
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		37
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		40
Caffeine 25 mg/ml 48 hrs, 4-day adult		12
Paraquat 5 mM 48 hrs, 4-day adult		31
Paraquat 10 mM 48 hrs, 4-day adult		26
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		12
cold shock, 4-day adult		6
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		47
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		37
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		40
Caffeine 25 mg/ml 48 hrs, 4-day adult		12
Paraquat 5 mM 48 hrs, 4-day adult		31
Paraquat 10 mM 48 hrs, 4-day adult		26
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		12
cold shock, 4-day adult		6
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		47
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		37
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		40
Caffeine 25 mg/ml 48 hrs, 4-day adult		12
Paraquat 5 mM 48 hrs, 4-day adult		31
Paraquat 10 mM 48 hrs, 4-day adult		26
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		12
cold shock, 4-day adult		6
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		(47)
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		(37)
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(40)
Caffeine 25 mg/ml 48 hrs, 4-day adult		12
Paraquat 5 mM 48 hrs, 4-day adult		31
Paraquat 10 mM 48 hrs, 4-day adult		26
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		12
cold shock, 4-day adult		6
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		47
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		37
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		40
Caffeine 25 mg/ml 48 hrs, 4-day adult		12
Paraquat 5 mM 48 hrs, 4-day adult		31
Paraquat 10 mM 48 hrs, 4-day adult		26
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		12
cold shock, 4-day adult		6
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		47
Copper 0.5 mM 12 hrs, larvae L3		1
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		10
Ethanol 2.5% 3 hrs, larvae L3		37
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		40
Caffeine 25 mg/ml 48 hrs, 4-day adult		12
Paraquat 5 mM 48 hrs, 4-day adult		31
Paraquat 10 mM 48 hrs, 4-day adult		26
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(Graveley et al., 2011)

mE1_20_mRNA_expression_cluster_04

External Data & Images

partially lethal - majority die

BDGP expression data

•

LD29481

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0000376

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG2714-RB

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

cell lethal | germline clone

crm⁷

lethal | larval stage | recessive

lethal | maternal effect | polyphasic

crm⁹

lethal | pupal stage | recessive

lethal | recessive

viable

crm^EY05302

viable, with Scer\GAL4^pnr-MD237

crm^GD1409

viable | female | poor

crm²

viable | male | poor

crm^swa

viable | male | reduced

crm²

Sterility

Allele

female fertile | partially

crm²

female fertile | poor

crm²

female sterile

fertile

crm^EY05302

male fertile

crm^swa

male fertile | partially

crm²

male fertile | reduced

crm²

male sterile

sterile

crm¹

Other Phenotypes

Allele

body color defective

crm^swa

eye color defective

crm^swa

size defective, with Scer\GAL4^bi-omb-Gal4

crm^{Del-V.Scer\UAS.T:Avic\GFP-YFP.Venus}

suppressor of variegation, with Df(4)ED6366

crm³²

suppressor of variegation, with P{hsp26-pt-T}118E-10

crm³²

suppressor of variegation | dominant, with In(1)w^m4h

crm^sa

visible

visible | adult stage, with Scer\GAL4^da.G32

visible | adult stage, with Scer\GAL4^pnr-MD237

crm^{Del-V.Scer\UAS.T:Avic\GFP-YFP.Venus}

visible | adult stage | temperature conditional

crm³²

visible | recessive

crm²

wild-type

crm^+t14

Phenotype manifest in

Allele

adult abdomen, with Scer\GAL4^da.G32

crm^{Del-V.Scer\UAS.T:Avic\GFP-YFP.Venus}

adult abdomen, with Scer\GAL4^pnr-MD237

crm^{Del-V.Scer\UAS.T:Avic\GFP-YFP.Venus}

adult abdomen | temperature conditional

crm³²

adult thorax, with Scer\GAL4^pnr-MD237

crm^{Del-V.Scer\UAS.T:Avic\GFP-YFP.Venus}

crm^swa

mesothoracic leg

crm⁷

metathoracic leg

crm⁷

ovariole | germline clone

crm⁹

crm⁷

prothoracic tarsal segment 2

crm^swa

sex comb

sex comb & mesothoracic tarsal segment 2

sex comb & prothoracic tarsal segment 2

sex comb | ectopic

crm⁷

sex comb | supernumerary, with Scer\GAL4^da.G32

sex comb | supernumerary, with Scer\GAL4^pnr-MD237

crm^{Del-V.Scer\UAS.T:Avic\GFP-YFP.Venus}

sex comb | supernumerary | temperature conditional

crm³²

tarsal segment 2

crm²

third segment of antenna

wing

wing disc, with Scer\GAL4^bi-omb-Gal4

crm^{Del-V.Scer\UAS.T:Avic\GFP-YFP.Venus}

wing margin | temperature conditional

crm³²

Classical Alleles ( 30 )

For All Classical Alleles Show

Allele of crm	Mutagen	Stocks	Known lesion
crm⁷	X ray	2	--
crm^EY05302	P-element activity	1	--
crm^f01351	piggyBac transposase	1	--
crm^sa2	spontaneous	1	--
crm¹⁰	ethyl methanesulfonate 2-chloroethyl methanesulfonate	0	--
crm¹¹	2-chloroethyl methanesulfonate	0	--
crm¹²	p-N,N-di-(2-chloroethyl)aminophenylethylamine	0	--
crm¹³	p-N,N-di-(2-chloroethyl)amino-L-phenylalanine	0	--
crm¹⁴	p-N,N-di-(2-chloroethyl)amino-DL-phenylalanine	0	--
crm¹⁵	S-2-chloroethylcysteine	0	--
crm¹⁶	methyl methanesulfonate	0	--
crm¹	32P	0	--
crm²	p-N,N-di-(2-chloroethyl)amino-L-phenylalanine	0	--
crm³²	P-element activity	0	Yes
crm³	X ray	0	--
crm⁴	X ray	0	--
crm⁵	X ray	0	--
crm⁶	X ray	0	--
crm⁸	spontaneous	0	--
crm⁹	ethyl methanesulfonate	0	--
crm^e51	ethyl methanesulfonate	0	--
crm^GG01400		0	--
crm^m25	methyl methanesulfonate	0	--
crm^M37	methyl methanesulfonate	0	--
crm^m45	methyl methanesulfonate	0	--
crm^m55	methyl methanesulfonate	0	--
crm^NKu1	urethane	0	--
crm^sa	spontaneous	0	--
crm^swa2	ethyl methanesulfonate	0	--
crm^swa	2-chloroethyl methanesulfonate	0	--

Alleles Carried on Transgenic Constructs ( 18 )

For All Alleles Carried on Transgenic Constructs Show

Allele of crm	Mutagen	Stocks	Known lesion
crm^GD1409	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
crm^GL00306	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
crm^JF01296	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
crm^KK107246	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
crm^+t14	in vitro construct - genomic fragment	0	Yes
crm^AM	in vitro construct - genomic fragment in vitro construct - site directed mutagenesis	0	Yes
crm^AT	in vitro construct - genomic fragment in vitro construct - site directed mutagenesis	0	Yes
crm^{Del-I.Scer\UAS.T:Avic\GFP-YFP.Venus}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion	0	Yes
crm^{Del-II.Scer\UAS.T:Avic\GFP-YFP.Venus}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion	0	Yes
crm^{Del-III.Scer\UAS.T:Avic\GFP-YFP.Venus}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion	0	Yes
crm^{Del-IV.Scer\UAS.T:Avic\GFP-YFP.Venus}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion	0	Yes
crm^{Del-V.Scer\UAS.T:Avic\GFP-YFP.Venus}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion	0	Yes
crm^{Del-VI.Scer\UAS.T:Avic\GFP-YFP.Venus}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion	0	Yes
crm^{Del-VII.Scer\UAS.T:Avic\GFP-YFP.Venus}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion	0	Yes
crm^GM	in vitro construct - genomic fragment in vitro construct - site directed mutagenesis	0	Yes
crm^GT	in vitro construct - genomic fragment in vitro construct - site directed mutagenesis	0	Yes
crm^NIG.2714R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
crm^rescue-	in vitro construct - deletion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(1)62d18

Df(1)64c4

Df(1)202

(Thierry-Mieg, 1982, Lin and Wolfner, 1989)

Df(1)HM3

(Kramers et al., 1983)

Df(1)HM406

(Kramers et al., 1983)

Df(1)LCD

(Yamamoto et al., 2000)

Df(1)N-8

Df(1)TEM75

(Lim and Snyder, 1974)

Df(1)TEM202

(Liu and Lim, 1975, Paradi et al., 1983, Kramers et al., 1983, Kramers et al., 1991)

Df(1)TEM501

(Lim and Snyder, 1974, Kramers et al., 1983, Kramers et al., 1991)

Df(1)w68a

Df(1)w70j

Df(1)w70k

Df(1)w71b

Df(1)w-72C2

(Pastink et al., 1987)

Df(1)w258-11

Df(1)w258-42

(Rayle and Green, 1968, Judd et al., 1972, Thierry-Mieg, 1982, Lin and Wolfner, 1989)

Df(1)w258-45

Df(1)w258-48

(Kramers et al., 1983, Kramers et al., 1991)

Df(1)w-DZL-1

Df(1)w-DZL-3

Df(1)w-N71a

(Judd et al., 1972, Lim and Snyder, 1974, Liu and Lim, 1975, Thierry-Mieg, 1982, Paradi et al., 1983, Kramers et al., 1983, Kramers et al., 1991, Nomura and Kurokawa, 1997)

Df(1)w-rJ1

Df(1)w-rJ2

Df(1)z-deb65

Df(1)z-deb72

In(1)w^-70L26.3

(Judd et al., 1972, Kramers et al., 1983, Kramers et al., 1991)

Duplicated in

Dp(1;2;Y)w⁺

Dp(1;2)w⁺70h

Dp(1;3)N^264-58

Dp(1;3)w⁺67k

Dp(1;3)w^m49a

Dp(1;3)w^m76

(Green, 2005.9.1)

Dp(1;3)w^vco

(Rayle and Green, 1968)

Dp(1;4)w^m65g

Dp(1;Y)y²67g19.1

(Lefevre, 1981)

Not disrupted in

Df(1)7

Df(1)62g18

(Judd et al., 1972, Thierry-Mieg, 1982, Lin and Wolfner, 1989)

Df(1)64f1

Df(1)64j4

(Judd et al., 1972, Thierry-Mieg, 1982, Lin and Wolfner, 1989)

Df(1)65j26

Df(1)304

Df(1)K95

Df(1)L126

(Lim and Snyder, 1974, Kramers et al., 1991)

Df(1)N-8

(Kramers et al., 1983)

Df(1)N-54l9

(Kramers et al., 1991)

Df(1)N-264-105

(Kramers et al., 1991)

Df(1)Pgd-kz

(Kramers et al., 1991)

Df(1)Sp2F-3A

(Schalet, 1986)

Df(1)TEM1

Df(1)TEM6

(Lim and Snyder, 1974)

Df(1)TEM7

(Lim and Snyder, 1974, Liu and Lim, 1975, Thierry-Mieg, 1982, Paradi et al., 1983, Kramers et al., 1983, Kramers et al., 1991)

Df(1)TEM304

(Lim and Snyder, 1974, Liu and Lim, 1975, Paradi et al., 1983, Kramers et al., 1983, Kramers et al., 1991)

Df(1)w55j

(Lin and Wolfner, 1989)

Df(1)w67k30

(Kramers et al., 1983, Kramers et al., 1991)

Df(1)w70e

Df(1)w-DZL-2

Df(1)w-DZL-14

(Montgomery et al., 1991)

Df(1)w-EM22

Df(1)X12

(Judd et al., 1972, Thierry-Mieg, 1982, Yamamoto et al., 1997)

Df(1)X22

Df(1)z-deb3

Df(1)z-deb42

Df(1)z-deb54

Df(1)z-deb59

Df(1)z-deb62

Df(1)z-deb81

Df(1)z-deb85

Df(1)z-deb87

Df(1)z-deb92

Df(1)z-deb311

Df(1)z-deb511

Df(1)z-deb910

(Kramers et al., 1983, Kramers et al., 1991)

Not duplicated in

Dp(1;2)51b

Dp(1;3)w^vco

(Schalet, 1986)

Tp(1;2)w⁺1

Tp(1;2)w⁺4

Tp(1;2)w⁺5

Tp(1;2)w⁺6

Tp(1;3)w⁺2

Tp(1;3)w⁺3

Tp(1;3)w⁺7

P{Mae-UAS.6.11}crm^GG01400

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{UAS-crm.Del-I.Venus}

P{UAS-crm.Del-II.Venus}

P{UAS-crm.Del-III.Venus}

P{UAS-crm.Del-IV.Venus}

P{UAS-crm.Del-V.Venus}

P{UAS-crm.Del-VI.Venus}

P{UAS-crm.Del-VII.Venus}

characterization construct

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EPgy2}crm^EY05302

miscellaneous insertions

PBac{WH}crm^f01351

Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )

Terms Based on Experimental Evidence ( 3 terms )

Molecular Function

CV term

References

chromatin binding

inferred from direct assay

Biological Process

CV term

References

segment specification

inferred from mutant phenotype

(assigned by UniProtKB)

Cellular Component

CV term

References

nucleus

inferred from direct assay

(assigned by UniProtKB)

Terms Based on Predictions or Assertions ( 2 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

regulation of transcription, DNA-dependent

References

DNA replication

non-traceable author statement

(Harr, 2001.3.24)

non-traceable author statement

(assigned by UniProtKB)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

crm allele

Gene

References

crm⁷

mus209

External Data

y¹ P{EPgy2}crm^EY05302 w^67c23

BioGRID - A database of protein and genetic interactions

•

57799

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0000376

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

31268

Orthologs

OrthoDB Orthologs (38) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6KKZNG

Dipteran inclusive ortholog search

EOG66T38S

Insect inclusive ortholog search

EOG6G1KBF

Arthropod inclusive ortholog search

EOG6VHHNG

Metazoa inclusive ortholog search

EOG671697

Orthologs in Drosophila Species (EOG6KKZNG)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA15444

Drosophila persimilis

Dper\GL19806

Drosophila willistoni

Dwil\GK25402

Drosophila virilis

Dvir\GJ18529

Drosophila mojavensis

Dmoj\GI10971

Orthologs in non-Drosophila Dipterans (EOG66T38S)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL014683

Anopheles gambiae

Malaria mosquito

Agam\AGAP001029

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ014021

Orthologs in non-Dipteran Insects (EOG6G1KBF)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB45695

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG012147

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH11172

Atta cephalotes

Leafcutter ant

Acep\ACEP18306

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO19623

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL16775

Linepithema humile

Argentine ant

Lhum\LH20377

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB17764

Solenopsis invicta

Red fire ant

Sinv\SINV23275

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000215

Bombyx mori

Silkmoth

Bmor\BGIBMGA014363

Pediculus humanus

Human body louse

Phum\PHUM117620

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_07439

Orthologs in non-Insect Arthropods (EOG6VHHNG)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\Efcab2

Ixodes scapularis

Deer tick

Isca\ISCW017333

Orthologs in non-Arthropod Metazoa (EOG671697)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\R151.8

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_003831gn

Danio rerio

Zebrafish

Drer\si:dkey-91d10.8

Xenopus tropicalis

Western clawed frog

Xtro\cramp1l

Gallus gallus

Domestic chicken

Ggal\CRAMP1L

Mus musculus

House mouse

Mmus\Cramp1l

Rattus norvegicus

Norway rat

Rnor\Cramp1l

Homo sapiens

Human

Hsap\CRAMP1L

Homo sapiens

Human

Hsap\ENSG00000261732

Human Orthologs (2)

Gene

OMIM

HGNC

Hsap\CRAMP1L

Hsap\ENSG00000261732

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA15444

Drosophila persimilis

Dper\GL19806

Drosophila willistoni

Dwil\GK25402

Drosophila virilis

Dvir\GJ18529

Drosophila mojavensis

Dmoj\GI10971

Drosophila grimshawi

Dgri\GH12275

Stocks & Reagents

Stocks Listed in FlyBase ( 9 )

Bloomington

16650

35387

y¹ sc^* v¹; P{TRiP.GL00306}attP2

9144

y¹ crm^sa2 sn³; Dp(1;3)w^m76

31338

y¹ v¹; P{TRiP.JF01296}attP2/TM3, Ser¹

4281

crm⁷ N^spl-1 sn³/FM7a/Dp(1;2;Y)w⁺

Harvard

PBac{WH}crm^f01351

Kyoto

107666

crm⁷, N^spl-1 sn³/FM7a/Dp(1;2;Y)w⁺

VDRC

v100245

P{KK107246}VIE-260B

v6308

w¹¹¹⁸; P{GD1409}v6308

Genomic Clones ( 1 )

BACR30B01

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 38 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

LD29481

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

DRSC - Results from RNAi screens.

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FBgn0000376

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

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31268

Antibody Information

polyclonal

Other Information

Discoverer

Bateman.

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: crm CG2714

(Deng, 1999.11)

Source for database merge of

Additional comments

l(1)zw9, sa: sparse arista and swa: swollen antennae. These are now designated alleles of crm.

Other Comments

Mutants exhibit typical Pc-group phenotypes, including homeotic transformations and modification of PEV.

A member of the Pc group. The genetic interaction between crm and mus209 highlight possible interactions between Pc-group mediated gene silencing and DNA replication.

crm is allelic with 'sa'.