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Gene Dmel\crn

General Information
Symbol	Dmel\crn	Species	D. melanogaster
Name	crooked neck	Annotation symbol	CG3193
Feature type	protein_coding_gene	FlyBase ID	FBgn0000377
Gene Model Status	Current	Stock availability	11 publicly available
Also Known As	yok, l(1)2, EG:30B8.1, l30
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	2F2-2F2	Sequence location	X:2,141,627..2,144,016 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene crooked neck is referred to in FlyBase by the symbol Dmel\crn (CG3193, FBgn0000377). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: oenocyte development; centrosome duplication; Malpighian tubule morphogenesis; glial cell migration; axon ensheathment; regulation of alternative mRNA splicing, via spliceosome; neurogenesis. 45 alleles are reported. The phenotypes of these alleles are annotated with: embryonic/larval oenocyte precursor; Malpighian tubule; glial cell; embryonic/larval glial cell; embryonic Malpighian tubule; peripheral glial cell; embryo. It has one annotated transcript and one annotated polypeptide. Protein features are: RNA-processing protein, HAT helix; Tetratricopeptide repeat-containing domain; Tetratricopeptide-like helical. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1635659_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of crn. Gene sequence location is X:2141627..2144015.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	crn: crooked neck Hemizygous embryos from heterozygous mothers show twisted phenotype; crn3/Df(1)64c18 and crn4/Df(1)64c18 exhibit a similar but more extreme phenotype, suggesting that they are hypomorphic. Both alleles lethal in female germ-line clones. yok: yolky Mutants are embryonic lethals with an undispersed yolk plug and no visible Malpighian tubules. Cuticle phenotype normal.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 2F2-2F2   Limits computationally determined from genome sequence between P{EP}EP1460&P{EP}CG2924EP1596 and P{EP}EP1606
Experimentally Determined Cytological Location
Cytogenetic map Notes References 2E3-2F1   (determined by in situ hybridisation)   (Vieira et al., 1997) 2E2-2F3   (determined by in situ hybridisation)   (Haenlin et al., 1985)
Experimentally Determined Recombination Data
Location
Left of (cM)	l(1)2Fd (Gvozdev et al., 1977)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\crn for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.51
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) crn-RA FBtr0070418  NM_057770   2332   702
Additional Transcript Data & Comments
Reported size (kB)	2.3 (northern blot) (Zhang et al., 1991) 2.1 (unknown) (Haenlin et al., 1985)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein crn-PA   FBpp0070402   84.3   702   5.71     NP_477118   AAF45760
Additional Polypeptide Data & Comments
Reported size (kDa)	702 (aa) (Zhang et al., 1991)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • RNA-processing protein, HAT helix (IPR003107) Tetratricopeptide repeat-containing domain (IPR013026) Tetratricopeptide-like helical (IPR011990)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA735662   AA803385   AE014298 AAF45760   AI259262   AI260996   AI455194   AI513687   AL009195 CAA15705   AY051666 AAK93090   BG636851   BI165319   BI212957   BI213889   BI232992   BI241528   BI243867   BI352913   BI357900   BI487224   BI568708   BI575242   BI575360   BI622429   BX057573   CK657870   CO263801   CO263931   CO290979   EC060815   EC221150   EC230908   EL875565   X58374 CAA41263
UniProtKB/Swiss-Prot	P17886
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference adult stage | female     (Haenlin et al., 1985) in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism | ubiquitous   (Zhang et al., 1991) embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
	crn transcripts are present in adult female RNA. (Haenlin et al., 1985) crn transcripts are expressed at fairly constant levels in all stages of development tested. In situ hybridization shows that it is uniformly distributed in embryos. (Zhang et al., 1991)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism | ubiquitous   (Edenfeld et al., 2006) embryonic stage 11 -- 15   glioblast   (Edenfeld et al., 2006) embryonic stage 16 -- 17   embryonic/larval glial cell   (Edenfeld et al., 2006) larval stage   ventral thoracic disc | restricted   (Edenfeld et al., 2006)
Additional Descriptive Data
	crn is predominantly localized in the nucleus, but is detected in low levels in the cytoplasm. (Edenfeld et al., 2006)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
catalytic step 2 spliceosome inferred from direct assay (Herold et al., 2009) centrosome inferred from direct assay (Müller et al., 2010) mitotic spindle inferred from direct assay (Müller et al., 2010) nuclear speck inferred from direct assay (Raisin-Tani and Leopold, 2002) precatalytic spliceosome inferred from direct assay (Herold et al., 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    281.725 Larval Midgut    80.3 Larval Hindgut    115.5 Larval Malpighian Tubules    135.8 Larval Fat Body    93.8 Larval Salivary Gland    132.2 Larval Trachea    158.35 Larval Carcass    117.125 Adult Head    111.8 Adult Eye    168.7 Adult Brain    143.4 Adult Thoracic-Abdominal Ganglion    143.8 Adult Crop    123.6 Adult Midgut    80.9 Adult Hindgut    119.7 Adult Malpighian Tubules    181.4 Adult Fat Body    125.8 Adult Salivary Gland    134.9 Adult Heart    100.25 Adult VirginFemale Spermatheca    165 Adult InseminatedFemale Spermatheca    176 Adult Ovary    195.4 Adult Testis    30.1 Adult Male Accessory Gland    160.8 Adult Carcass    92.1 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    281.725 Larval Midgut    80.3 Larval Hindgut    115.5 Larval Malpighian Tubules    135.8 Larval Fat Body    93.8 Larval Salivary Gland    132.2 Larval Trachea    158.35 Larval Carcass    117.125 Adult Head    111.8 Adult Eye    168.7 Adult Brain    143.4 Adult Thoracic-Abdominal Ganglion    143.8 Adult Crop    123.6 Adult Midgut    80.9 Adult Hindgut    119.7 Adult Malpighian Tubules    181.4 Adult Fat Body    125.8 Adult Salivary Gland    134.9 Adult Heart    100.25 Adult VirginFemale Spermatheca    165 Adult InseminatedFemale Spermatheca    176 Adult Ovary    195.4 Adult Testis    30.1 Adult Male Accessory Gland    160.8 Adult Carcass    92.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    281.725 Larval Midgut    80.3 Larval Hindgut    115.5 Larval Malpighian Tubules    135.8 Larval Fat Body    93.8 Larval Salivary Gland    132.2 Larval Trachea    158.35 Larval Carcass    117.125 Adult Head    111.8 Adult Eye    168.7 Adult Brain    143.4 Adult Thoracic-Abdominal Ganglion    143.8 Adult Crop    123.6 Adult Midgut    80.9 Adult Hindgut    119.7 Adult Malpighian Tubules    181.4 Adult Fat Body    125.8 Adult Salivary Gland    134.9 Adult Heart    100.25 Adult VirginFemale Spermatheca    165 Adult InseminatedFemale Spermatheca    176 Adult Ovary    195.4 Adult Testis    30.1 Adult Male Accessory Gland    160.8 Adult Carcass    92.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    281.725 Larval Midgut    80.3 Larval Hindgut    115.5 Larval Malpighian Tubules    135.8 Larval Fat Body    93.8 Larval Salivary Gland    132.2 Larval Trachea    158.35 Larval Carcass    117.125 Adult Head    111.8 Adult Eye    168.7 Adult Brain    143.4 Adult Thoracic-Abdominal Ganglion    143.8 Adult Crop    123.6 Adult Midgut    80.9 Adult Hindgut    119.7 Adult Malpighian Tubules    181.4 Adult Fat Body    125.8 Adult Salivary Gland    134.9 Adult Heart    100.25 Adult VirginFemale Spermatheca    165 Adult InseminatedFemale Spermatheca    176 Adult Ovary    195.4 Adult Testis    30.1 Adult Male Accessory Gland    160.8 Adult Carcass    92.1 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    281.725 Larval Midgut    80.3 Larval Hindgut    115.5 Larval Malpighian Tubules    135.8 Larval Fat Body    93.8 Larval Salivary Gland    132.2 Larval Trachea    158.35 Larval Carcass    117.125 Adult Head    111.8 Adult Eye    168.7 Adult Brain    143.4 Adult Thoracic-Abdominal Ganglion    143.8 Adult Crop    123.6 Adult Midgut    80.9 Adult Hindgut    119.7 Adult Malpighian Tubules    181.4 Adult Fat Body    125.8 Adult Salivary Gland    134.9 Adult Heart    100.25 Adult VirginFemale Spermatheca    165 Adult InseminatedFemale Spermatheca    176 Adult Ovary    195.4 Adult Testis    30.1 Adult Male Accessory Gland    160.8 Adult Carcass    92.1 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    281.725 Larval Midgut    80.3 Larval Hindgut    115.5 Larval Malpighian Tubules    135.8 Larval Fat Body    93.8 Larval Salivary Gland    132.2 Larval Trachea    158.35 Larval Carcass    117.125 Adult Head    111.8 Adult Eye    168.7 Adult Brain    143.4 Adult Thoracic-Abdominal Ganglion    143.8 Adult Crop    123.6 Adult Midgut    80.9 Adult Hindgut    119.7 Adult Malpighian Tubules    181.4 Adult Fat Body    125.8 Adult Salivary Gland    134.9 Adult Heart    100.25 Adult VirginFemale Spermatheca    165 Adult InseminatedFemale Spermatheca    176 Adult Ovary    195.4 Adult Testis    30.1 Adult Male Accessory Gland    160.8 Adult Carcass    92.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    281.725 Larval Midgut    80.3 Larval Hindgut    115.5 Larval Malpighian Tubules    135.8 Larval Fat Body    93.8 Larval Salivary Gland    132.2 Larval Trachea    158.35 Larval Carcass    117.125 Adult Head    111.8 Adult Eye    168.7 Adult Brain    143.4 Adult Thoracic-Abdominal Ganglion    143.8 Adult Crop    123.6 Adult Midgut    80.9 Adult Hindgut    119.7 Adult Malpighian Tubules    181.4 Adult Fat Body    125.8 Adult Salivary Gland    134.9 Adult Heart    100.25 Adult VirginFemale Spermatheca    165 Adult InseminatedFemale Spermatheca    176 Adult Ovary    195.4 Adult Testis    30.1 Adult Male Accessory Gland    160.8 Adult Carcass    92.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    281.725 Larval Midgut    80.3 Larval Hindgut    115.5 Larval Malpighian Tubules    135.8 Larval Fat Body    93.8 Larval Salivary Gland    132.2 Larval Trachea    158.35 Larval Carcass    117.125 Adult Head    111.8 Adult Eye    168.7 Adult Brain    143.4 Adult Thoracic-Abdominal Ganglion    143.8 Adult Crop    123.6 Adult Midgut    80.9 Adult Hindgut    119.7 Adult Malpighian Tubules    181.4 Adult Fat Body    125.8 Adult Salivary Gland    134.9 Adult Heart    100.25 Adult VirginFemale Spermatheca    165 Adult InseminatedFemale Spermatheca    176 Adult Ovary    195.4 Adult Testis    30.1 Adult Male Accessory Gland    160.8 Adult Carcass    92.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    111.8   NA  Eye    168.7   NA  Brain    143.4   281.725  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    143.8   NA  Crop    123.6   80.3  Midgut    80.9   115.5  Hindgut    119.7   135.8  Malpighian Tubules    181.4   93.8  Fat Body    125.8   132.2  Salivary Gland    134.9   NA  Heart    100.25   158.35  Trachea    NA   NA  VirginFemale Spermatheca    165   NA  InseminatedFemale Spermatheca    176   NA  Ovary    195.4   NA  Testis    30.1   NA  Male Accessory Gland    160.8   117.125  Carcass    92.1 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    16 central nervous system, larvae L3    23 central nervous system, pupae P8    8 head, virgin 1-day female    9 head, virgin 4-day female    9 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    8 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    13 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    1 carcass, larvae L3 wandering    17 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    6 ovary, virgin 4-day female    21 ovary, mated 4-day female    20 testis, mated 4-day male    7 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    16 central nervous system, larvae L3    23 central nervous system, pupae P8    8 head, virgin 1-day female    9 head, virgin 4-day female    9 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    8 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    13 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    1 carcass, larvae L3 wandering    17 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    6 ovary, virgin 4-day female    21 ovary, mated 4-day female    20 testis, mated 4-day male    7 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    16 central nervous system, larvae L3    23 central nervous system, pupae P8    8 head, virgin 1-day female    9 head, virgin 4-day female    9 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    8 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    13 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    1 carcass, larvae L3 wandering    17 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    6 ovary, virgin 4-day female    21 ovary, mated 4-day female    20 testis, mated 4-day male    7 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    16 central nervous system, larvae L3    23 central nervous system, pupae P8    8 head, virgin 1-day female    9 head, virgin 4-day female    9 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    8 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    13 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    1 carcass, larvae L3 wandering    17 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    6 ovary, virgin 4-day female    21 ovary, mated 4-day female    20 testis, mated 4-day male    7 accessory gland, mated 4-day male    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    16 central nervous system, larvae L3    23 central nervous system, pupae P8    8 head, virgin 1-day female    9 head, virgin 4-day female    9 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    8 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    13 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    1 carcass, larvae L3 wandering    17 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    6 ovary, virgin 4-day female    21 ovary, mated 4-day female    20 testis, mated 4-day male    7 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    16 central nervous system, larvae L3    23 central nervous system, pupae P8    8 head, virgin 1-day female    9 head, virgin 4-day female    9 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    8 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    13 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    1 carcass, larvae L3 wandering    17 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    6 ovary, virgin 4-day female    21 ovary, mated 4-day female    20 testis, mated 4-day male    7 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    16 central nervous system, larvae L3    23 central nervous system, pupae P8    8 head, virgin 1-day female    9 head, virgin 4-day female    9 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    8 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    13 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    1 carcass, larvae L3 wandering    17 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    6 ovary, virgin 4-day female    21 ovary, mated 4-day female    20 testis, mated 4-day male    7 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    16 central nervous system, larvae L3    23 central nervous system, pupae P8    8 head, virgin 1-day female    9 head, virgin 4-day female    9 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    8 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    13 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    1 carcass, larvae L3 wandering    17 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    6 ovary, virgin 4-day female    21 ovary, mated 4-day female    20 testis, mated 4-day male    7 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    65 embryo 04-06hr    58 embryo 06-08hr    55 embryo 08-10hr    45 embryo 10-12hr    36 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    23 embryo 22-24hr    19 larva L1    15 larva L2    14 larva L3 12hr old    11 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    18 white prepupae 24hr    20 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    7 adult male 01day    9 adult male 05day    9 adult male 30day    10 adult female 01day    18 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (39) embryo 02-04hr  (65) embryo 04-06hr  (58) embryo 06-08hr  (55) embryo 08-10hr  (45) embryo 10-12hr  (36) embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    23 embryo 22-24hr    19 larva L1    15 larva L2    14 larva L3 12hr old    11 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    18 white prepupae 24hr    20 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    7 adult male 01day    9 adult male 05day    9 adult male 30day    10 adult female 01day    18 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    65 embryo 04-06hr    58 embryo 06-08hr    55 embryo 08-10hr    45 embryo 10-12hr    36 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    23 embryo 22-24hr    19 larva L1    15 larva L2    14 larva L3 12hr old    11 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    18 white prepupae 24hr    20 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    7 adult male 01day    9 adult male 05day    9 adult male 30day    10 adult female 01day    18 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    65 embryo 04-06hr    58 embryo 06-08hr    55 embryo 08-10hr    45 embryo 10-12hr    36 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    23 embryo 22-24hr    19 larva L1    15 larva L2    14 larva L3 12hr old    11 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    18 white prepupae 24hr    20 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    7 adult male 01day    9 adult male 05day    9 adult male 30day    10 adult female 01day    18 adult female 05day    22 adult female 30day    23 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    65 embryo 04-06hr    58 embryo 06-08hr    55 embryo 08-10hr    45 embryo 10-12hr    36 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    23 embryo 22-24hr    19 larva L1    15 larva L2    14 larva L3 12hr old    11 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    18 white prepupae 24hr    20 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    7 adult male 01day    9 adult male 05day    9 adult male 30day    10 adult female 01day    18 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (39) embryo 02-04hr  (65) embryo 04-06hr  (58) embryo 06-08hr  (55) embryo 08-10hr  (45) embryo 10-12hr  (36) embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    23 embryo 22-24hr    19 larva L1    15 larva L2    14 larva L3 12hr old    11 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    18 white prepupae 24hr    20 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    7 adult male 01day    9 adult male 05day    9 adult male 30day    10 adult female 01day    18 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    65 embryo 04-06hr    58 embryo 06-08hr    55 embryo 08-10hr    45 embryo 10-12hr    36 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    23 embryo 22-24hr    19 larva L1    15 larva L2    14 larva L3 12hr old    11 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    18 white prepupae 24hr    20 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    7 adult male 01day    9 adult male 05day    9 adult male 30day    10 adult female 01day    18 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    65 embryo 04-06hr    58 embryo 06-08hr    55 embryo 08-10hr    45 embryo 10-12hr    36 embryo 12-14hr    23 embryo 14-16hr    23 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    23 embryo 22-24hr    19 larva L1    15 larva L2    14 larva L3 12hr old    11 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    18 white prepupae 24hr    20 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    7 adult male 01day    9 adult male 05day    9 adult male 30day    10 adult female 01day    18 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    86 Schneider line 2 Sg4    34 embryonic 1182-4H    38 embryonic GM2    20 embryonic Kc167    23 embryonic S1    41 embryonic S3    25 leg disc CME L1    32 wing disc CME-W2    20 wing disc ML-DmD8    28 wing disc ML-DmD9    35 wing disc ML-DmD16-c3    39 wing disc ML-DmD21    47 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    47 eye-antennal disc ML-DmD11    29 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    33 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    44 tumorous blood cells mbn2    22 ovary fGS/OSS    33 ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (86) Schneider line 2 Sg4  (34) embryonic 1182-4H  (38) embryonic GM2    20 embryonic Kc167    23 embryonic S1  (41) embryonic S3    25 leg disc CME L1  (32) wing disc CME-W2    20 wing disc ML-DmD8  (28) wing disc ML-DmD9  (35) wing disc ML-DmD16-c3  (39) wing disc ML-DmD21  (47) wing disc ML-DmD32    25 haltere disc ML-DmD17-c3  (47) eye-antennal disc ML-DmD11  (29) antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1  (33) CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2  (44) tumorous blood cells mbn2    22 ovary fGS/OSS  (33) ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    86 Schneider line 2 Sg4    34 embryonic 1182-4H    38 embryonic GM2    20 embryonic Kc167    23 embryonic S1    41 embryonic S3    25 leg disc CME L1    32 wing disc CME-W2    20 wing disc ML-DmD8    28 wing disc ML-DmD9    35 wing disc ML-DmD16-c3    39 wing disc ML-DmD21    47 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    47 eye-antennal disc ML-DmD11    29 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    33 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    44 tumorous blood cells mbn2    22 ovary fGS/OSS    33 ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    86 Schneider line 2 Sg4    34 embryonic 1182-4H    38 embryonic GM2    20 embryonic Kc167    23 embryonic S1    41 embryonic S3    25 leg disc CME L1    32 wing disc CME-W2    20 wing disc ML-DmD8    28 wing disc ML-DmD9    35 wing disc ML-DmD16-c3    39 wing disc ML-DmD21    47 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    47 eye-antennal disc ML-DmD11    29 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    33 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    44 tumorous blood cells mbn2    22 ovary fGS/OSS    33 ovary OSC    17 ovary OSS    13 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    86 Schneider line 2 Sg4    34 embryonic 1182-4H    38 embryonic GM2    20 embryonic Kc167    23 embryonic S1    41 embryonic S3    25 leg disc CME L1    32 wing disc CME-W2    20 wing disc ML-DmD8    28 wing disc ML-DmD9    35 wing disc ML-DmD16-c3    39 wing disc ML-DmD21    47 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    47 eye-antennal disc ML-DmD11    29 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    33 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    44 tumorous blood cells mbn2    22 ovary fGS/OSS    33 ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (86) Schneider line 2 Sg4  (34) embryonic 1182-4H  (38) embryonic GM2    20 embryonic Kc167    23 embryonic S1  (41) embryonic S3    25 leg disc CME L1  32 wing disc CME-W2    20 wing disc ML-DmD8  28 wing disc ML-DmD9  (35) wing disc ML-DmD16-c3  (39) wing disc ML-DmD21  (47) wing disc ML-DmD32    25 haltere disc ML-DmD17-c3  (47) eye-antennal disc ML-DmD11  29 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1  (33) CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2  (44) tumorous blood cells mbn2    22 ovary fGS/OSS  (33) ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    86 Schneider line 2 Sg4    34 embryonic 1182-4H    38 embryonic GM2    20 embryonic Kc167    23 embryonic S1    41 embryonic S3    25 leg disc CME L1    32 wing disc CME-W2    20 wing disc ML-DmD8    28 wing disc ML-DmD9    35 wing disc ML-DmD16-c3    39 wing disc ML-DmD21    47 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    47 eye-antennal disc ML-DmD11    29 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    33 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    44 tumorous blood cells mbn2    22 ovary fGS/OSS    33 ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    86 Schneider line 2 Sg4    34 embryonic 1182-4H    38 embryonic GM2    20 embryonic Kc167    23 embryonic S1    41 embryonic S3    25 leg disc CME L1    32 wing disc CME-W2    20 wing disc ML-DmD8    28 wing disc ML-DmD9    35 wing disc ML-DmD16-c3    39 wing disc ML-DmD21    47 wing disc ML-DmD32    25 haltere disc ML-DmD17-c3    47 eye-antennal disc ML-DmD11    29 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    33 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    44 tumorous blood cells mbn2    22 ovary fGS/OSS    33 ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    21 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    22 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_17 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • LD21701 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000377 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3193-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele cell lethal | germline clone crn4 lethal crn5 crn6 crn7 crn8 crn9 crnGD13667 lethal, with Scer\GAL4pnr-MD237 crnGD3204 crnGD10412 crnGD13667 lethal | dominant crn3 lethal | embryonic stage | recessive crn3 crn4 crnyok-EH160 crnyok-EH272 crnyok-EH328 crnyok-EH352 lethal | germline clone crn3 crn4 lethal | pupal stage, with Scer\GAL4pnr-MD237 crnGD13667 crnGD15799 lethal | recessive crn1 crn3 crn4 crn5 crn6 crn7 crn8 crn9 crn10 crn11 crn12 crn13 crn14 crn15 crn16 crn17 crn18 crn19 viable, with Scer\GAL4pnr-MD237 crnGD9664 Other Phenotypes Allele neuroanatomy defective crn8H7 crn15H6 crn26H8 crnunspecified neuroanatomy defective | larval stage crnGD13667 wild-type crn+tcos9 crn+tKpn13 crn+tXba15 Phenotype manifest in Allele embryo crn1 embryonic/larval glial cell crn26H8 crn8H7 embryonic/larval neuroblast | larval stage crnGD13667 embryonic/larval oenocyte precursor crn15H6 embryonic Malpighian tubule crnEH3 ganglion mother cell | larval stage crnGD13667 glial cell crnunspecified Malpighian tubule crnyok-EH160 crnyok-EH272 crnyok-EH328 peripheral glial cell crn15H6
Classical Alleles ( 28 )
For All Classical Alleles Show
Allele of crn Class Mutagen Stocks Known lesion crnyok-EH352 ethyl methanesulfonate 2 -- crn10 ethyl methanesulfonate 0 -- crn11 ethyl methanesulfonate 0 -- crn12 ethyl methanesulfonate 0 -- crn13 ethyl methanesulfonate 0 -- crn14 ethyl methanesulfonate 0 -- crn15 ethyl methanesulfonate 0 -- crn15H6 ethyl methanesulfonate 0 Yes crn16 gamma ray 0 -- crn17 hycanthon methanesulfonate 0 -- crn18 hycanthon methanesulfonate 0 -- crn19 hycanthon methanesulfonate 0 -- crn1 X ray 0 -- crn26H8 ethyl methanesulfonate 0 Yes crn2 X ray 0 -- crn3 hypomorphic allele - genetic evidence X ray 0 Yes crn4 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- crn5 ethyl methanesulfonate 0 -- crn6 ethyl methanesulfonate 0 -- crn7 ethyl methanesulfonate 0 -- crn8 ethyl methanesulfonate 0 -- crn8H7 ethyl methanesulfonate 0 -- crn9 ethyl methanesulfonate 0 -- crnEH3 0 -- crnunspecified 0 -- crnyok-EH160 ethyl methanesulfonate 0 -- crnyok-EH272 ethyl methanesulfonate 0 -- crnyok-EH328 ethyl methanesulfonate 0 --
Alleles Carried on Transgenic Constructs ( 17 )
For All Alleles Carried on Transgenic Constructs Show
Allele of crn Class Mutagen Stocks Known lesion crnGD10412 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes crnGD13667 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes crnGD15799 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes crnGD3204 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes crnGD9664 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes crnHM05215 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes crnKK100412 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes crn+tcos9 in vitro construct - genomic fragment 0 Yes crn+tKpn13 in vitro construct - genomic fragment 0 Yes crn+tXba15 in vitro construct - genomic fragment 0 Yes crndsRNA.WIZ.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes crnNIG.3193R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes crnScer\UAS.cEa in vitro construct - regulatory fusion 0 Yes crnScer\UAS.T:Myr-Src64B in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes crntHa in vitro construct - genomic fragment 0 Yes crntHb in vitro construct - genomic fragment 0 Yes crntHc in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)2F1-3A4 (Haenlin et al., 1985) Df(1)64c18 (Gvozdev et al., 1975, Gvozdev et al., 1977, Perrimon et al., 1984, Alatortsev and Tolchkov, 1985, Perrimon et al., 1985) Df(1)278.4B1 (Perrimon et al., 1984, Perrimon et al., 1985) Df(1)Pgd-kz (Gvozdev et al., 1977, Kramers et al., 1983, Perrimon et al., 1984, Alatortsev and Tolchkov, 1985, Perrimon et al., 1985, Kramers et al., 1991) Df(1)pn7a (Perrimon et al., 1984, Alatortsev and Tolchkov, 1985, Perrimon et al., 1985) Df(1)TEM1 (Gvozdev et al., 1975, Gvozdev et al., 1977, Alatortsev and Tolchkov, 1985, Kramers et al., 1991) Df(1)TEM304 (Gvozdev et al., 1975, Gvozdev et al., 1977, Kramers et al., 1983, Perrimon et al., 1984, Alatortsev and Tolchkov, 1985, Perrimon et al., 1985, Kramers et al., 1991) Df(1)TEM501 (Gvozdev et al., 1975, Gvozdev et al., 1977, Kramers et al., 1983, Alatortsev and Tolchkov, 1985, Kramers et al., 1991) In(1)C99Lwm4R (Perrimon et al., 1985)
Duplicated in	Dp(1;2;Y)w+ (Kramers et al., 1983, Eberl and Hilliker, 1988, Kramers et al., 1991, Edenfeld et al., 2006) Dp(1;3)DC039 (Popodi et al., 2010-, Venken et al., 2010) Dp(1;3)wvco (Perrimon et al., 1985) Dp(1;Y)w+303 (Perrimon et al., 1985) Dp(1;Y)y2sc (Eberl and Hilliker, 1988)
Not disrupted in	Df(1)2F1-3A4 (Perrimon et al., 1984, Perrimon et al., 1985) Df(1)62g18 (Perrimon et al., 1984) Df(1)dor2T (Alatortsev and Tolchkov, 1985) Df(1)JA52 (Perrimon et al., 1985) Df(1)JC19 (Perrimon et al., 1984) Df(1)JC105 (Perrimon et al., 1984, Perrimon et al., 1985) Df(1)l24 (Perrimon et al., 1984, Perrimon et al., 1985) Df(1)L271 (Perrimon et al., 1984, Perrimon et al., 1985) Df(1)N-8 (Kramers et al., 1991) Df(1)N-54l9 (Kramers et al., 1991) Df(1)N-264-105 (Kramers et al., 1991) Df(1)Pgd35 (Perrimon et al., 1984) Df(1)pn38 (Alatortsev and Tolchkov, 1985, Perrimon et al., 1985) Df(1)TEM7 (Kramers et al., 1991) Df(1)TEM75 (Perrimon et al., 1984) Df(1)TEM202 (Kramers et al., 1991) Df(1)w67k30 (Kramers et al., 1991) Df(1)w258-48 (Kramers et al., 1991) Df(1)w-rJ1 (Kramers et al., 1983, Kramers et al., 1991) Df(1)X12 (Perrimon et al., 1984) In(1)pn45 (Alatortsev and Tolchkov, 1985) T(1;3)pn12 (Alatortsev and Tolchkov, 1985)
Not duplicated in	Dp(1;2)51b (Kramers et al., 1991) Dp(1;2)w+64b (Eberl and Hilliker, 1988) Dp(1;f)101 (Eberl and Hilliker, 1988) Dp(1;Y)dor3T (Alatortsev and Tolchkov, 1985) Dp(1;Y)y243T (Alatortsev and Tolchkov, 1985) Dp(1;Y)y267g19.1 (Eberl and Hilliker, 1988) Dp(1;Y)y2sc (Eberl and Hilliker, 1988)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD3204} NA P{GD9664} NA P{GD10412} NA P{GD13667} NA P{GD15799} NA P{KK100412} NA P{NIG.3193R} NA P{TRiP.HM05215} NA P{UAS-crn.E} NA P{UAS-crn.Myr} NA P{UAS-crn.RNAi} NA characterization construct P{fs(1)K10+tHb} NA P{fs(1)K10+tHd} NA P{fs(1)K10tHa} NA P{Kpn13} NA P{Xba15} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 19 unique terms )
Terms Based on Experimental Evidence ( 12 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
axon ensheathment inferred from mutant phenotype (Edenfeld et al., 2006) centrosome duplication inferred from mutant phenotype (Müller et al., 2010) glial cell migration inferred from mutant phenotype (Edenfeld et al., 2006) Malpighian tubule morphogenesis inferred from mutant phenotype (Jack and Myette, 1999) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) oenocyte development inferred from mutant phenotype (Edenfeld et al., 2006) regulation of alternative mRNA splicing, via spliceosome inferred from mutant phenotype (Park et al., 2004, Volohonsky et al., 2007, Katzenberger et al., 2009)
Cellular Component
CV term Evidence References
catalytic step 2 spliceosome inferred from direct assay (Herold et al., 2009) centrosome inferred from direct assay (Müller et al., 2010) mitotic spindle inferred from direct assay (Müller et al., 2010) nuclear speck inferred from direct assay (Raisin-Tani and Leopold, 2002) precatalytic spliceosome inferred from direct assay (Herold et al., 2009)
Terms Based on Predictions or Assertions ( 7 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
central nervous system development non-traceable author statement (Swiss-Prot Project Members, 1992.3.1) mRNA splicing, via spliceosome inferred by curator from GO:0071011|GO:0071013 (Herold et al., 2009) inferred from sequence or structural similarity with NCBI:NP_013218 (Mount and Salz, 2000) neuroblast proliferation non-traceable author statement (Swiss-Prot Project Members, 1992.3.1) neuron fate commitment non-traceable author statement (Swiss-Prot Project Members, 1992.3.1) peripheral nervous system development non-traceable author statement (Swiss-Prot Project Members, 1992.3.1)
Cellular Component
CV term Evidence References
nucleus inferred by curator from GO:0000381 (Park et al., 2004) non-traceable author statement (Swiss-Prot Project Members, 1992.3.1) spliceosomal complex inferred from sequence or structural similarity with NCBI:NP_013218 (Mount and Salz, 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
crn - Ars2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Atx2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - B52 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Bx42 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) crn - CG10077 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG10418 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG10628 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG10641 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG1091 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG11107 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG11266 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG11505 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG12128 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG13298 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG13900 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG14786 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG15084 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG15161 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG15525 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG15784 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG16940 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG17768 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG1785 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG2021 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG3091 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG31368 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG3224 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG3436 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) crn - CG3605 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG3689 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG3817 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG3862 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG4611 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG4612 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG4849 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) crn - CG5746 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG6015 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) crn - CG6197 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) crn - CG6674 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG6701 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG7137 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG7146 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG7185 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG7878 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG8021 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG8728 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG9667 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) crn - CG9684 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - CG9775 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Cbp80 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Cdc5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Clbn anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Cypl anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Dp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Jupiter anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - LRP1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Map205 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Mtr3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - NHP2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Pitslre anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Prosα5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Prp8 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) crn - Rbp1-like anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - RpS11 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - RpS27A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Rrp45 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Rrp46 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - SC35 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Saf-B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - SmD2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - SmD3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Srp54 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Syp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Tango4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Thiolase anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - U2A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Upf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Wbp2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - Zn72D anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - bsf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - btz anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - cora anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - eIF4AIII anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - exu anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - fs(1)K10 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - l(1)10Bb experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) crn - l(1)G0020 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - l(3)72Ab anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - mRpL3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - mTerf3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - nito anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - noi anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - pUf68 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - qkr58E-3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - snRNP-U1-70K anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - snf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - spoon anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) crn - vig anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions crn allele Gene References crn3 Ubx (Burnette et al., 1999) crn4 Ubx (Burnette et al., 1999)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 57745 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0000377 DroID - A comprehensive database of gene and protein interactions. • FBgn0000377 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31208
Orthologs
OrthoDB Orthologs (41) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG60023R Dipteran inclusive ortholog search EOG6KD6V5 Insect inclusive ortholog search EOG6GXDGS Arthropod inclusive ortholog search EOG6KWH7T Metazoa inclusive ortholog search EOG6FZ41G
Orthologs in Drosophila Species (EOG60023R)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\crn     Drosophila simulans   Dsim\GD24613 Y   Drosophila sechellia   Dsec\GM18901 Y   Drosophila erecta   Dere\GG12634 Y   Drosophila yakuba   Dyak\GE16963 Y   Drosophila ananassae   Dana\GF22206 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA22854 Y   Drosophila persimilis   Dper\GL13392 Y   Drosophila willistoni   Dwil\GK16333 Y   Drosophila virilis   Dvir\GJ16476 Y   Drosophila mojavensis   Dmoj\GI21455 Y   Drosophila grimshawi   Dgri\GH12311 Y
Orthologs in non-Drosophila Dipterans (EOG6KD6V5)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL009383   Anopheles gambiae Malaria mosquito  Agam\AGAP001879   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ008391
Orthologs in non-Dipteran Insects (EOG6GXDGS)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\crn   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG009514   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH26718   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH16286   Atta cephalotes Leafcutter ant  Acep\ACEP16662   Camponotus floridanus Florida carpenter ant  Cflo\CFLO19088   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21732   Linepithema humile Argentine ant  Lhum\LH10403   Pogonomyrmex barbatus Red harvester ant  Pbar\PB11632   Solenopsis invicta Red fire ant  Sinv\SINV13228   Acyrthosiphon pisum Pea aphid  Apim\ACYPI007623   Bombyx mori Silkmoth  Bmor\BGIBMGA008459   Pediculus humanus Human body louse  Phum\PHUM169990   Tribolium castaneum Red flour beetle  Tcas\TC004395
Orthologs in non-Insect Arthropods (EOG6KWH7T)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_201152   Ixodes scapularis Deer tick  Isca\ISCW011304
Orthologs in non-Arthropod Metazoa (EOG6FZ41G)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\M03F8.3   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_012748gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_018535gn   Danio rerio Zebrafish  Drer\crnkl1   Xenopus tropicalis Western clawed frog  Xtro\crnkl1   Gallus gallus Domestic chicken  Ggal\CRNKL1   Mus musculus House mouse  Mmus\Gm4782   Mus musculus House mouse  Mmus\Crnkl1   Rattus norvegicus Norway rat  Rnor\Crnkl1   Homo sapiens Human  Hsap\CRNKL1
Human Orthologs (1)
Gene OMIM HGNC Hsap\CRNKL1
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD24613 Drosophila sechellia Dsec\GM18901 Drosophila erecta Dere\GG12634 Drosophila yakuba Dyak\GE16963 Drosophila ananassae Dana\GF22206 Drosophila pseudoobscura pseudoobscura Dpse\GA22854 Drosophila persimilis Dper\GL13392 Drosophila willistoni Dwil\GK16333 Drosophila virilis Dvir\GJ16476 Drosophila mojavensis Dmoj\GI21455 Drosophila grimshawi Dgri\GH12311
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Bloomington	29535 y1 sc* v1; P{TRiP.HM05215}attP2 4292 y1 crnyok-EH352/C(1)DX, y1 f1/Dp(1;Y)y2sc
Kyoto	107673 y1 crnyok-EH352/C(1)DX, y1 f1/Dp(1;Y)y2sc
VDRC	v103393 P{KK100412}VIE-260B v24340 w1118 P{GD9664}v24340 v6932 w1118; P{GD3204}v6932 v25918 w1118; P{GD10412}v25918 v25919 w1118; P{GD10412}v25919 v39335 w1118; P{GD15799}v39335 v28815 w1118; P{GD13667}v28815/CyO v28816 w1118; P{GD13667}v28816/CyO
Genomic Clones ( 2 )
	BACR13K19 BACR48O22 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 28 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD21701
Other clones	DPiM_LD21701
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH26740 GM10758 GM21268 LP02638 LP05055 RE05174 RE18240 RE19525 RE29102 RE29253 RE38621 RE41669 RE44522 RE70746 RH29789 RH30930 RH40383 RH54302 SD14656
Other clones	28543 28815 CK01.97D.D7 EK075716 EK136510 EK136914 IP05561
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0000377 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31208
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: crn CG3193 (Deng, 1999.11)
Source for database merge of
Additional comments	Release 2 annotation CG18842 split into CG3193 (crn) and CG3191 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
Other Comments
	Identified as a potential component of the hh signalling pathway in a genome-wide RNAi screen. dsRNA made from templates generated with primers directed affects the extent of expression of a hh signaling reporter construct in Clone 8 cells. (Nybakken et al., 2005) Muscle phenotype of mutants studied using polarised light microscopy and antibody staining to detect Mhc-lacZ reporter gene expression in muscles. (Drysdale et al., 1993) Molecular analysis of crn indicates it is related to a family of genes required for normal progression through the cell cycle. Sequence similarity and mutant phenotype suggest crn encodes a protein required for cell division. Zygotic expression of crn is required to complete late rounds of cell division in the embryonic nervous system. (Zhang et al., 1991) A screen for X-linked genes that affect embryo morphology revealed yok. (Eberl and Hilliker, 1988) A saturation analysis of the 2D3--2F5 region detected the crn complementation group. (Alatortsev and Tolchkov, 1985) Germline clonal analysis indicates that this locus is required for female germ line viability. (Perrimon et al., 1984) crn3/Df(1)64c18 and crn4/Df(1)64c18 exhibit a similar but more extreme phenotype, suggesting that they are hypomorphic. Both alleles lethal in female germ-line clones
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057770 RefSeq (Proteins) • NP_477118 DDBJ / EMBL / GenBank • AA735662 AAF45760 AAK93090 AI260996 AI455194 AL009171 AL009195 AY051666 CAA15705 CAA41263 X58374 Entrez Gene - A searchable database of RefSeq genes. • 31208 UniProtKB/Swiss-Prot • P17886
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • RNA-processing protein, HAT helix (IPR003107) Tetratricopeptide repeat-containing domain (IPR013026) Tetratricopeptide-like helical (IPR011990) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD21701
Linkouts
	BioGRID - A database of protein and genetic interactions • 57745 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0000377 DroID - A comprehensive database of gene and protein interactions. • FBgn0000377 DRSC - Results from RNAi screens. • FBgn0000377 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000377 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3193-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0000377 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31208 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/crookedneck.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31208 modMine - Data generated by the modENCODE project. • FBgn0000377
Synonyms & Secondary IDs ( 18 )
Reported As
Symbol Synonym	62D9.a (Lai et al., 2001) CG3193 (Benos, 2000.12.13, Mount and Salz, 2000, Nybakken et al., 2005, Keleman et al., 2009.8.5, Perkins et al., 2009, Herold et al., 2009, McIntyre et al., 2006) CG18842   crn (Kruger, 2005, Lecuyer et al., 2007, Edenfeld et al., 2006, Liu et al., 2009, Herold et al., 2009, Popodi et al., 2010-, McIntyre et al., 2006, Neumüller et al., 2011, Park et al., 2011, Japanese National Institute of Genetics, 2012.5.21, Venken et al., 2010) Crn (Nybakken et al., 2005, Volohonsky et al., 2007, Katzenberger et al., 2009) EG:30B8.1 (Benos et al., 2001, Benos, 2000.12.13, ) l30 (Kramers et al., 1991, Kramers et al., 1983) l(1)2 (Mechler, 1990, Gateff and Mechler, 1989, Gateff, 1978) l(1)2Fa   l(1)CRN (Perrimon et al., 1985) l(1)crn   l(1)N7   N5 (Gvozdev et al., 1975) N10 (Alatortsev and Tolchkov, 1985) RC63 (Kramers et al., 1991) yok (Harbecke and Lengyel, 1995, Drysdale et al., 1993, Merriam et al., 1991, )
Name Synonym	crooked neck (Park et al., 2011) yolky (Skaer, 1993, )
Secondary FlyBase IDs
	FBgn0004044 FBgn0042162
References ( 73 )
Generate a list of	All references relating to this gene ( 73 )
List References by type	Research paper  ( 38 ) Supplementary material  ( 6 ) Review  ( 9 ) Personal communication to FlyBase  ( 8 ) FlyBase analysis  ( 3 ) Stock list  ( 1 ) Curated genome annotation  ( 3 ) Protein sequence record  ( 1 ) Website  ( 1 ) Book  ( 1 ) Spoof  ( 1 ) DNA/RNA sequence record  ( 1 )
Recent research papers ( 2 )
	Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Park et al., 2011, PLoS Genet. 7(8): e1002241 Specification of Drosophila corpora cardiaca neuroendocrine cells from mesoderm is regulated by Notch signaling. [FBrf0215231] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...