FB2020_03, released Jun 16, 2020

Gene: Dmel\crn

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General Information
Symbol
Dmel\crn
Species
D. melanogaster
Name
crooked neck
Annotation Symbol
CG3193
Feature Type
protein_coding_gene
FlyBase ID
FBgn0000377
Gene Model Status
Current
Stock Availability
16 publicly available
Gene Snapshot
crooked neck (crn) encodes a component of the splicing complex involved in axon ensheathment, glial cell migration and Malpighian tubule morphogenesis. [Date last reviewed: 2018-09-06]
Other Summaries
Also Known As

yok, l(1)2, EG:30B8.1 , yolky, l30

Key Links
Genomic Location
Cytogenetic map
2F2-2F2
Sequence location
X:2,247,594..2,249,983 [-]
Recombination map

1-0.9

RefSeq locus
NC_004354 REGION:2247594..2249983
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (16 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
RNA binding
inferred from sequence or structural similarity with HGNC:15762
(FlyBase Curators, 2008--)
Biological Process (7 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
axon ensheathment
inferred from mutant phenotype
(Edenfeld et al., 2006)
glial cell migration
inferred from mutant phenotype
(Edenfeld et al., 2006)
Malpighian tubule morphogenesis
inferred from mutant phenotype
(Jack and Myette, 1999)
oenocyte development
inferred from mutant phenotype
(Edenfeld et al., 2006)
regulation of alternative mRNA splicing, via spliceosome
inferred from high throughput mutant phenotype
(Park et al., 2004)
inferred from mutant phenotype
(Katzenberger et al., 2009, Volohonsky et al., 2007)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
mRNA splicing, via spliceosome
inferred by curator from GO:0071011,GO:0071013
(Herold et al., 2009)
inferred from biological aspect of ancestor with PANTHER:PTN000133723
(assigned by GO_Central )
(Gaudet et al., 2011)
spliceosomal complex assembly
inferred from biological aspect of ancestor with PANTHER:PTN000133724
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (8 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
catalytic step 2 spliceosome
inferred from high throughput direct assay
(Herold et al., 2009)
cytosol
inferred from direct assay
(Liu et al., 2014)
nuclear speck
inferred from direct assay
(Raisin-Tani and Leopold, 2002)
nucleus
inferred from direct assay
(Liu et al., 2014)
precatalytic spliceosome
inferred from high throughput direct assay
(Herold et al., 2009)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
catalytic step 2 spliceosome
inferred from biological aspect of ancestor with PANTHER:PTN000133724
(assigned by GO_Central )
(Gaudet et al., 2011)
nucleus
inferred by curator from GO:0000381
(Park et al., 2004)
post-mRNA release spliceosomal complex
inferred from biological aspect of ancestor with PANTHER:PTN000133724
(assigned by GO_Central )
(Gaudet et al., 2011)
Prp19 complex
inferred from biological aspect of ancestor with PANTHER:PTN000133724
(assigned by GO_Central )
(Gaudet et al., 2011)
U2-type catalytic step 2 spliceosome
inferred from biological aspect of ancestor with PANTHER:PTN000133724
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the crooked-neck family. (P17886)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
SPLICEOSOME COMPLEX B -
Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. After the assembly of complex A on pre-mRNA, the U4-U6 and U5 snRNPs are recruited as a preassembled tri-snRNP to form complex B. U5 snRNP binds exons at the 5' site. (Adapted from PMID:24452469 and PMID:23118483).
SPLICEOSOME COMPLEX C -
Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex B undergoes a number of structural rearrangements and U4 and U1 snRNPs dissociate to generate complex C, the catalytic spliceosome. (Adapted from PMID:24452469 and PMID:23118483).
SPLICEOSOME COMPLEX P -
Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex P, the post-spliceosomal complex, is formed after the catalytic removal of the intron by complex C. Complex P contains the lariat intron and spliced exons. (Adapted from PMID:24452469 and PMID:23118483).
Protein Function (UniProtKB)
May be involved in pre-mRNA splicing process (PubMed:12163015, PubMed:2044955). Involved in embryonic neurogenesis and cell rearrangement during Malpighian tubule morphogenesis (PubMed:2044955, PubMed:10502111).
(UniProt, P17886)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
crn: crooked neck
Hemizygous embryos from heterozygous mothers show twisted phenotype; crn3/Df(1)64c18 and crn4/Df(1)64c18 exhibit a similar but more extreme phenotype, suggesting that they are hypomorphic. Both alleles lethal in female germ-line clones.
yok: yolky
Mutants are embryonic lethals with an undispersed yolk plug and no visible Malpighian tubules. Cuticle phenotype normal.
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\crn or the JBrowse view of Dmel\crn for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.51

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
crn-RA
FBtr0070418
NM_057770
2332
702
Additional Transcript Data and Comments
Reported size (kB)

2.3 (northern blot)

(Zhang et al., 1991)

2.1 (unknown)

(Haenlin et al., 1985)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
crn-PA
FBpp0070402
84.3
702
5.71
NP_477118
AAF45760
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

702 (aa)

(Zhang et al., 1991)
Comments
External Data
Subunit Structure (UniProtKB)

Colocalizes with a complex containing snRNP proteins.

(UniProt, P17886)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\crn using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | female
(Haenlin et al., 1985)
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
organism | ubiquitous
(Zhang et al., 1991)
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
organism | ubiquitous
(Fisher et al., 2012)
Additional Descriptive Data

crn transcripts are expressed at fairly constant levels in all stages of development tested. In situ hybridization shows that it is uniformly distributed in embryos.

(Zhang et al., 1991)

crn transcripts are present in adult female RNA.

(Haenlin et al., 1985)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
ventral thoracic disc | restricted
(Edenfeld et al., 2006)
embryonic stage
organism | ubiquitous
(Edenfeld et al., 2006)
embryonic stage 11 -- 15
glioblast
(Edenfeld et al., 2006)
embryonic stage 16 -- 17
embryonic/larval glial cell
(Edenfeld et al., 2006)
Additional Descriptive Data

crn is predominantly localized in the nucleus, but is detected in low levels in the cytoplasm.

(Edenfeld et al., 2006)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
catalytic step 2 spliceosome
inferred from high throughput direct assay
(Herold et al., 2009)
cytosol
inferred from direct assay
(Liu et al., 2014)
nuclear speck
inferred from direct assay
(Raisin-Tani and Leopold, 2002)
nucleus
inferred from direct assay
(Liu et al., 2014)
precatalytic spliceosome
inferred from high throughput direct assay
(Herold et al., 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\crn in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 28 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 21 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of crn
Transgenic constructs containing regulatory region of crn
Deletions and Duplications ( 41 )
Disrupted in
Df(1)2F1-3A4
(Haenlin et al., 1985)
Df(1)64c18
(Alatortsev and Tolchkov, 1985, Perrimon et al., 1985, Perrimon et al., 1984, Gvozdev et al., 1977, Gvozdev et al., 1975)
Df(1)278.4B1
(Perrimon et al., 1985, Perrimon et al., 1984)
Df(1)Pgd-kz
(Kramers et al., 1991, Alatortsev and Tolchkov, 1985, Perrimon et al., 1985, Perrimon et al., 1984, Kramers et al., 1983, Gvozdev et al., 1977)
Df(1)pn7a
(Alatortsev and Tolchkov, 1985, Perrimon et al., 1985, Perrimon et al., 1984)
Df(1)TEM1
(Kramers et al., 1991, Alatortsev and Tolchkov, 1985, Gvozdev et al., 1977, Gvozdev et al., 1975)
Df(1)TEM304
(Kramers et al., 1991, Alatortsev and Tolchkov, 1985, Perrimon et al., 1985, Perrimon et al., 1984, Kramers et al., 1983, Gvozdev et al., 1977, Gvozdev et al., 1975)
Df(1)TEM501
(Kramers et al., 1991, Alatortsev and Tolchkov, 1985, Kramers et al., 1983, Gvozdev et al., 1977, Gvozdev et al., 1975)
In(1)C99Lwm4R
(Perrimon et al., 1985)
Duplicated in
Dp(1;2;Y)w+
(Edenfeld et al., 2006, Kramers et al., 1991, Eberl and Hilliker, 1988, Kramers et al., 1983)
Dp(1;3)DC039
(Popodi et al., 2010-, Venken et al., 2010)
Dp(1;3)wvco
(Perrimon et al., 1985)
Dp(1;Y)w+303
(Perrimon et al., 1985)
Dp(1;Y)y2sc
(Eberl and Hilliker, 1988)
Not disrupted in
Df(1)2F1-3A4
(Perrimon et al., 1985, Perrimon et al., 1984)
Df(1)62g18
(Perrimon et al., 1984)
Df(1)dor2T
(Alatortsev and Tolchkov, 1985)
Df(1)JA52
(Perrimon et al., 1985)
Df(1)JC19
(Perrimon et al., 1984)
Df(1)JC105
(Perrimon et al., 1985, Perrimon et al., 1984)
Df(1)l24
(Perrimon et al., 1985, Perrimon et al., 1984)
Df(1)L271
(Perrimon et al., 1985, Perrimon et al., 1984)
Df(1)N-8
(Kramers et al., 1991)
Df(1)N-54l9
(Kramers et al., 1991)
Df(1)N-264-105
(Kramers et al., 1991)
Df(1)Pgd35
(Perrimon et al., 1984)
Df(1)pn38
(Alatortsev and Tolchkov, 1985, Perrimon et al., 1985)
Df(1)TEM7
(Kramers et al., 1991)
Df(1)TEM75
(Perrimon et al., 1984)
Df(1)TEM202
(Kramers et al., 1991)
Df(1)w67k30
(Kramers et al., 1991)
Df(1)w258-48
(Kramers et al., 1991)
Df(1)w-rJ1
(Kramers et al., 1991, Kramers et al., 1983)
Df(1)X12
(Perrimon et al., 1984)
In(1)pn45
(Alatortsev and Tolchkov, 1985)
T(1;3)pn12
(Alatortsev and Tolchkov, 1985)
Not duplicated in
Dp(1;2)51b
(Kramers et al., 1991)
Dp(1;2)w+64b
(Eberl and Hilliker, 1988)
Dp(1;f)101
(Eberl and Hilliker, 1988)
Dp(1;Y)dor3T
(Alatortsev and Tolchkov, 1985)
Dp(1;Y)y243T
(Alatortsev and Tolchkov, 1985)
Dp(1;Y)y267g19.1
(Eberl and Hilliker, 1988)
Dp(1;Y)y2sc
(Eberl and Hilliker, 1988)
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
cell lethal | germline clone
lethal
lethal, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
lethal, with Scer\GAL4pnr-MD237
lethal, with Scer\GAL4tin.CΔ4
lethal | dominant
lethal | germline clone
lethal | pupal stage, with Scer\GAL4pnr-MD237
lethal | recessive
lethal - all die before end of embryonic stage | recessive
lethal - all die before end of pupal stage, with Scer\GAL4pnr-MD237
lethal - all die during larval stage, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
lethal - all die during larval stage, with Scer\GAL4Mef2.PR
lethal - all die during P-stage, with Scer\GAL4Mef2.PR
lethal - all die during pupal stage, with Scer\GAL4tin.CΔ4
partially lethal, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
partially lethal, with Scer\GAL4tin.CΔ4
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4pnr-MD237
Other Phenotypes
Allele
neuroanatomy defective
neuroanatomy defective | larval stage
short lived | heat sensitive, with Scer\GAL4tin.CΔ4
size defective | third instar larval stage, with Scer\GAL4Cg.PA
some die during embryonic stage | recessive
some die during pupal stage, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
some die during pupal stage, with Scer\GAL4pnr-MD237
wild-type
Phenotype manifest in
Allele
embryo
embryonic/larval fat body | third instar larval stage, with Scer\GAL4Cg.PA
embryonic/larval glial cell
embryonic/larval oenocyte precursor
embryonic/larval somatic muscle, with Scer\GAL4Mef2.PR
embryonic Malpighian tubule
ganglion mother cell | larval stage
glial cell
larval neuroblast | larval stage
Malpighian tubule
mitochondrion | third instar larval stage, with Scer\GAL4Cg.PA
peripheral glial cell
sarcomere, with Scer\GAL4Mef2.PR
Z disc, with Scer\GAL4Mef2.PR
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\CRNKL1
14 of 15
Yes
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Crnkl1
14 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (3)
Rnor\LOC102548514
12 of 13
Yes
Yes
Rnor\Crnkl1
5 of 13
No
Yes
Rnor\Crnkl1-ps1
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\crnkl1
9 of 12
Yes
Yes
Danio rerio (Zebrafish) (1)
Drer\crnkl1
9 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\M03F8.3
14 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (4)
Atha\AT5G45990
9 of 9
Yes
Yes
Atha\AT3G13210
8 of 9
No
Yes
Atha\AT5G41770
8 of 9
No
Yes
Atha\AT3G51110
3 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\CLF1
13 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\cwf4
11 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091903V3 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00005234
Drosophila simulans
Dsim\GD24613
Drosophila sechellia
Dsec\GM18901
Drosophila erecta
Dere\GG12634
Drosophila yakuba
Dyak\GE16963
Drosophila ananassae
Dana\GF22206
Drosophila pseudoobscura pseudoobscura
Dpse\GA22854
Drosophila persimilis
Dper\GL13392
Drosophila willistoni
Dwil\GK16333
Drosophila virilis
Dvir\GJ16476
Drosophila mojavensis
Dmoj\GI21455
Drosophila grimshawi
Dgri\GH12311
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501VB )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17006322
Glossina morsitans
Tsetse fly
Gmor\GMOY005795
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_03060
Mayetiola destructor
Hessian fly
Mdes\Mdes003696
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL009383
Anopheles darlingi
American malaria mosquito
Adar\ADAC010014
Anopheles gambiae
Malaria mosquito
Agam\AGAP001879
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ008391
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W029U )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA008459
Danaus plexippus
Monarch butterfly
Dple\DPOGS211365-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL002788
Apis florea
Little honeybee
Aflo\4500495636
Apis mellifera
Western honey bee
Amel\crn
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP22597
Bombus terrestris
Buff-tailed bumblebee
Bter\15201838
Linepithema humile
Argentine ant
Lhum\LH10403
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:002553
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG009514
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_08918
Tribolium castaneum
Red flour beetle
Tcas\TC004395
Pediculus humanus
Human body louse
Phum\PHUM169990
Rhodnius prolixus
Kissing bug
Rpro\RPRC005125
Cimex lectularius
Bed bug
Clec\CLEC007715
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI007623
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_02705
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01R1 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR005047
Strigamia maritima
European centipede
Smar\SMAR001165
Strigamia maritima
European centipede
Smar\SMAR005565
Ixodes scapularis
Black-legged tick
Isca\ISCW011304
Stegodyphus mimosarum
African social velvet spider
Smim\KFM59299.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur06g05280
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_201152
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G040G )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Crnkl1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Crnkl1_1
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000002709
Gallus gallus
Domestic chicken
Ggal\CRNKL1
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (2)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
CG6841
2 of 10
fand
2 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    CRNKL1; crooked neck pre-mRNA splicing factor 1
    14 of 15
    610952
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement
        View in Interactions Browser
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        how
        suppressible
        crn
        (Edenfeld et al., 2006)
        how
        enhanceable
        crn
        (Edenfeld et al., 2006)
        Ubx
        enhanceable
        crn
        (Burnette et al., 1999)
        External Data
        Subunit Structure (UniProtKB)
        Colocalizes with a complex containing snRNP proteins.
        (UniProt, P17886 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        X
        Recombination map

        1-0.9

        Cytogenetic map
        2F2-2F2
        Sequence location
        X:2,247,594..2,249,983 [-]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        2F2-2F2
        Limits computationally determined from genome sequence between P{EP}EP1460&P{EP}CG2924EP1596 and P{EP}EP1606
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        2E3-2F1
        (determined by in situ hybridisation)
        (Vieira et al., 1997)
        2E2-2F3
        (determined by in situ hybridisation)
        (Haenlin et al., 1985)
        Experimentally Determined Recombination Data
        Location

        1-0.9

        (FlyBase, 2016)

        1-0.2

        (Comeron et al., 2012)
        Left of (cM)
        (Gvozdev et al., 1977)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (16)
        Genomic Clones (15)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (29)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: crn CG3193

        (Deng, 1999.11)
        Source for database merge of
        Additional comments

        Release 2 annotation CG18842 split into CG3193 (crn) and CG3191 in release 3 of the genome annotation.

        (FlyBase Genome Annotators, 2002-2003)
        Other Comments

        Identified as a potential component of the hh signalling pathway in a genome-wide RNAi screen. dsRNA made from templates generated with primers directed affects the extent of expression of a hh signaling reporter construct in Clone 8 cells.

        (Nybakken et al., 2005)

        Muscle phenotype of mutants studied using polarised light microscopy and antibody staining to detect Mhc-lacZ reporter gene expression in muscles.

        (Drysdale et al., 1993)

        Molecular analysis of crn indicates it is related to a family of genes required for normal progression through the cell cycle. Sequence similarity and mutant phenotype suggest crn encodes a protein required for cell division. Zygotic expression of crn is required to complete late rounds of cell division in the embryonic nervous system.

        (Zhang et al., 1991)

        A screen for X-linked genes that affect embryo morphology revealed yok.

        (Eberl and Hilliker, 1988)

        A saturation analysis of the 2D3--2F5 region detected the crn complementation group.

        (Alatortsev and Tolchkov, 1985)

        Germline clonal analysis indicates that this locus is required for female germ line viability.

        (Perrimon et al., 1984)

        crn3/Df(1)64c18 and crn4/Df(1)64c18 exhibit a similar but more extreme phenotype, suggesting that they are hypomorphic. Both alleles lethal in female germ-line clones

        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 40 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DPiM - Drosophila Protein interaction map
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Synonyms and Secondary IDs (18)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (98)