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General Information
Symbol
Dmel\CycB
Species
D. melanogaster
Name
Cyclin B
Annotation Symbol
CG3510
Feature Type
FlyBase ID
FBgn0000405
Gene Model Status
Stock Availability
Gene Snapshot
Cyclin B (CycB) encodes a protein that binds to Cdk1, thereby promoting Cdk1's protein kinase activity. The complex CycB-Cdk1 complex induces the start of mitosis. During late metaphase of mitosis and continuing in G1, the product of CycB is rapidly degraded (involving APC/C and the proteasome), resulting in inactivation of Cdk1 protein kinase activity, and allowing termination of mitosis. [Date last reviewed: 2019-03-07]
Also Known As

Cyc B, Cyclin B1

Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:22,803,521..22,806,948 [-]
Recombination map

2-101

RefSeq locus
NT_033778 REGION:22803521..22806948
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (21 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000019791
(assigned by GO_Central )
Biological Process (11 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
Cellular Component (8 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
colocalizes_with mitotic spindle
inferred from direct assay
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (6 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN002468700
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000019791
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000019791
(assigned by GO_Central )
traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000019791
(assigned by GO_Central )
traceable author statement
Protein Family (UniProt)
Belongs to the cyclin family. Cyclin AB subfamily. (P20439)
Summaries
Protein Function (UniProtKB)
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
(UniProt, P20439)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
CycB: Cyclin B
Encodes cyclin, a molecule involved in the cell cycle; the pattern of transcription reflects this. Maternal message uniformly distributed in newly laid egg, but becomes concentrated at the posterior pole at the time of polar-nucleus migration; also evident in the cortex of the syncytial blastoderm. Larval message concentrated in the testis.
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
4

Please see the GBrowse view of Dmel\CycB or the JBrowse view of Dmel\CycB for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.41

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0071911
2633
530
FBtr0071913
2556
524
FBtr0071914
2548
500
FBtr0309858
2627
528
Additional Transcript Data and Comments
Reported size (kB)

2.3 (unknown)

2.7 (northern blot)

2.489 (longest cDNA)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0071822
59.3
530
10.23
FBpp0071824
58.6
524
10.23
FBpp0071825
55.9
500
10.18
FBpp0301592
59.1
528
10.23
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)

530 (aa); 64 (kD)

530 (aa); 65 (kD)

Comments
External Data
Subunit Structure (UniProtKB)

Interacts with the protein kinase Cdk1 to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex.

(UniProt, P20439)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CycB using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

antennal primordium

Comment: reported as procephalic ectoderm primordium

central brain primordium

Comment: reported as procephalic ectoderm primordium

visual primordium

Comment: reported as procephalic ectoderm primordium

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

organism | posterior

Comment: very strong in posterior cap

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

CycB expression in early embryos is diffusely concentrated at the anterior end of the embryo.

High levels of CycB transcripts are observed at times of rapid cell proliferation such as early and late embryo stages and at lower levels at other times. In preblastoderm embryos, CycB transcripts are detected throughout the cytoplasm with a higher concentration at the posterior end. During nuclear migration, CycB transcripts migrate in close association with the nuclei to the periphery and the mRNA at the posterior cap is incorporated into the forming pole cells. In somatic cells, signal decreases during blastoderm stages and increases again at gastrulation, at which time, transcripts are observed throughout the embryo. During germband retraction, CycB trancripts are observed in the developing nervous system but are no longer seen in epidermal cells that have completed their cell divisions.

CycB transcripts are concentrated around nuclei in syncytial embryos and then migrate with them to the cortex. They are concentrated in the regions around nuclei that are rich in microtubules. CycB transcripts were found to be concentrated at the posterior pole of early embryos and are thought to concentrate there during oogenesis. This posterior localization was abolished in mutants of stau, vas, spir, capu, and osk. Posterior localization of CycB transcripts is less striking in tud and vls mutants than in wild type embryos and indistinguishable from wild type in nos and pum mutants. No ectopic anterior expression of CycB was seen in BicD mutants.

Marker for
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

Post embryonic mitosis 16 CycB protein is detected primarily in the proliferating nervous system and is absent from most other embryonic tissues.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
colocalizes_with mitotic spindle
inferred from direct assay
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CycB in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 14 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 53 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of CycB
Transgenic constructs containing regulatory region of CycB
Deletions and Duplications ( 6 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
anaphase & condensed nuclear chromosome
anaphase & condensed nuclear chromosome | maternal effect
egg chamber & cell
egg chamber & mitotic cell cycle
onion stage spermatid & nuclear chromosome
onion stage spermatid & nucleus
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (19)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
11 of 15
Yes
Yes
1  
10 of 15
No
Yes
1  
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
No
1  
2 of 15
No
No
1  
2 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1  
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1  
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (18)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
11 of 15
Yes
Yes
10 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1  
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (19)
8 of 13
Yes
Yes
3 of 13
No
No
3 of 13
No
No
3 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
Yes
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (23)
10 of 12
Yes
Yes
7 of 12
No
Yes
6 of 12
No
Yes
3 of 12
No
No
3 of 12
No
No
3 of 12
No
No
3 of 12
No
Yes
2 of 12
No
No
2 of 12
No
No
2 of 12
No
Yes
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
Danio rerio (Zebrafish) (22)
11 of 15
Yes
Yes
6 of 15
No
Yes
4 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
3 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (15)
9 of 15
Yes
Yes
7 of 15
No
Yes
7 of 15
No
Yes
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (12)
7 of 9
Yes
Yes
6 of 9
No
Yes
5 of 9
No
Yes
5 of 9
No
Yes
4 of 9
No
Yes
4 of 9
No
Yes
4 of 9
No
Yes
4 of 9
No
Yes
3 of 9
No
Yes
3 of 9
No
Yes
1 of 9
No
No
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (9)
10 of 15
Yes
Yes
9 of 15
No
Yes
8 of 15
No
Yes
7 of 15
No
Yes
6 of 15
No
Yes
6 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (5)
7 of 12
Yes
Yes
7 of 12
Yes
Yes
7 of 12
Yes
Yes
2 of 12
No
Yes
1 of 12
No
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091905ZW )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915077U )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Glossina morsitans
Tsetse fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W07HL )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0E9B )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (6)
5 of 10
4 of 10
3 of 10
2 of 10
2 of 10
2 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    RNA-protein
    Physical Interaction
    Assay
    References
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Interacts with the protein kinase Cdk1 to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex.
    (UniProt, P20439 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map

    2-101

    Cytogenetic map
    Sequence location
    2R:22,803,521..22,806,948 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    59B2-59B2
    Limits computationally determined from genome sequence between P{PZ}blw03972 and P{EP}EP627
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    59A-59A
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (25)
    Genomic Clones (17)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (312)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: CycB CG3510

    Source for database merge of
    Additional comments
    Other Comments

    CycB promotes germline stem cell abscission.

    Phosphorylation by aurB inhibits CycB activity.

    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

    Temporal coordination of mitotic events involves inhibition of cytokinesis by CycB and CycB3 and punctual relief of inhibition by destruction of these Cyclins.

    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

    CycB destruction triggers changes to kinetochore behaviour essential for successful anaphase.

    Myb induces expression of CycB in the eye imaginal disc.

    CycB destruction is required for anaphase B, cytokinesis and for directional stability of univalent chromosome movements.

    A balance of CycB and CycA proteins seems necessary for proper coordination during the embryonic cycles.

    CycB regulates cytoskeletal changes while CycA regulates the nuclear changes in syncytial preblastoderm embryos.

    CycB3 and CycB are not essential for viability. Double and triple mutant analyses show that CycB3, CycB and CycA cooperate during mitosis.

    aly is required for CycB and twe expression in primary spermatocytes. Accumulation of twe and CycB proteins occurs just before the first meiotic division in wild type.

    Overexpression of a truncated form of CycB using the GAL4 system can produce adults with rough eyes, deformed wings, abnormal bristles and die within hours of emergence. Overexpression of full length CycB produces less severe cuticular defects.

    A deficiency of CycB causes a mitotic cycle delay at metaphase stages during blastoderm. Delay results in precocious expression of the knrl gene which compensates for partial loss of kni activity allowing kni mutant progeny to survive. Heterozygosity for CycB is sufficient for females to produce viable homozygous kni mutant progeny. Double heterozygotes for CycA and CycB increase the efficiency for kni mutant rescue suggesting the cyclins interact synergistically.

    Mutations in fzy result in metaphase arrest, neither CycA, CycB or CycB3 are degraded in this arrest. Comparison of the fzy mutant phenotype with the phenotype resulting from expression of N-terminal truncated CycA, CycB or CycB3 suggests that fzy is not only required for mitotic cyclin degradation because fzy mutations, but not truncated cyclins, block chromosome separation.

    During development changing levels of cell cycle regulators alters the rate limiting step and the mechanism governing progress of the cell cycle. Three phases of developmental progression of cell cycle regulation have been defined. The first seven cycles run in the presence of constitutively active cdc2. Later during cycles 8-13 increasing mitotic destruction of cyclins drives oscillations in cdc2 activity, cyclin accumulation becomes the rate limiting step for mitosis. Degradation of maternally supplied stg causes tyrosine dephosphorylation of cdc2 to become rate limiting for mitosis beginning in cycle 14.

    Translation of CycB RNA in the pole cells appears to be repressed until pole cells begin proliferation in the gonad.

    Maternal mRNA localises to the pole region of the embryo.

    polo gene product immunoprecipitated from single Drosophila embryos can phosphorylate casein in vitro, and the kinase activity peaks cyclically at late anaphase/telophase. This contrasts with the cycling of CycB associated p34cdc2 histone H1 kinase, which is maximal upon entry into mitosis during the rapid syncitial mitoses.

    The CycB product acts synergistically with CycA during the G2-M transition, and though not essential for mitosis, it is required for normal spindle formation.

    As part of an investigation into the effects of 37oC heat shock on G2-phase cycle 14 embryos, the degradation of cyclin A and cyclin B in the resulting synchronised mitotic domains was shown to be delayed. 37oC heat shock applied at S phase of cycle 14 causes cell cycle arrest with microtubules in an interphase-like state, and nuclei showing unusual chromatin condensation.

    Oocyte-specific accumulation of osk, CycB and 65F mRNAs is blocked by microtubule assembly inhibitors.

    Ecol\lacZ reporter gene constructs carrying the promoter of CycA and CycB have been used to identify a posterior localization sequence in CycB mRNA.

    Cyclin B distribution studied in Kc line where it is detected in prophase and metaphase, localised to 2 spots, then disappears during anaphase. In an acentriolar cell line made from the maternal haploid (mh) female sterile mutant Cyclin B does not localise to 2 spots per metaphase.

    CycA and CycB behaviour in syncytial embryos has been studied.

    Immunocytological approach is used to address the behaviour of essential mitotic gene products in embryonic cell cycles.

    CycB is not sufficient alone for mitosis. CycA and CycB are coexpressed in all proliferating cells throughout development. The regulation of CycB gene transcription is not affected by a premature stop to cell division in mutant embryos.

    Two independent mechanisms act to concentrate CycB transcripts at the posterior pole of the oocyte and at the cortex of the syncytial embryo. Posterior localization of CycB transcript is disrupted in mutant embryos that fail to form pole cells.

    Arrest of the cell cycle with microtubule destabilizing drugs demonstrated that CycB degradation is the key requirement for entry into anaphase.

    Encodes cyclin, a molecule involved in the cell cycle; the pattern of transcription reflects this. Maternal message uniformly distributed in newly laid egg, but becomes concentrated at the posterior pole at the time of polar-nucleus migration; also evident in the cortex of the syncytial blastoderm. Larval message concentrated in the testis.

    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 74 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DPiM - Drosophila Protein interaction map
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (24)
    Reported As
    Symbol Synonym
    CycB
    (Hinnant et al., 2020, Kohzaki et al., 2020, Okazaki et al., 2020, Archambault, 2019.12.8, Blake-Hedges and Megraw, 2019, Campbell, 2019.12.4, Duy Binh et al., 2019, Greenblatt et al., 2019, Grendler et al., 2019, Liao et al., 2019, Mouawad et al., 2019, Pavlova et al., 2019, Qiu et al., 2019, Rivera et al., 2019, Rotelli et al., 2019, Sechi et al., 2019, Aydogan et al., 2018, Cong et al., 2018, Drechsler et al., 2018, Eusebio et al., 2018, Flora et al., 2018, Flora et al., 2018, Hao et al., 2018, Kim et al., 2018, Otsuki and Brand, 2018, Vedelek et al., 2018, Wu et al., 2018, Hara et al., 2017, Li et al., 2017, Neuert et al., 2017, Romero-Pozuelo et al., 2017, Transgenic RNAi Project members, 2017-, Chandra et al., 2016, Guo et al., 2016, Kong et al., 2016, Sysoev et al., 2016, Varadarajan et al., 2016, Baker et al., 2015, Bergman et al., 2015, Blythe and Wieschaus, 2015, Défachelles et al., 2015, Liu and Lasko, 2015, Wang and Baker, 2015, Ayeni et al., 2014, Bischof, 2014.10.20, Gómez-Lamarca et al., 2014, Günesdogan et al., 2014, Wong et al., 2014, Zhang et al., 2014, Bonke et al., 2013, Chang et al., 2013, Kanda et al., 2013, Lu et al., 2013, Marzuk et al., 2013, Mathieu et al., 2013, Mishra et al., 2013, Neumüller et al., 2013, Schertel et al., 2013, Webber et al., 2013, Zhou and Luo, 2013, Zoranovic et al., 2013, Althoff et al., 2012, Domanitskaya and Schüpbach, 2012, Farrell et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Liu et al., 2012, Meier et al., 2012, Reich and Papoulas, 2012, San Juan et al., 2012, Xu et al., 2012, Chen et al., 2011, Doggett et al., 2011, Miles et al., 2011, Moon et al., 2011, Poulton et al., 2011, Richter et al., 2011, Beam and Moberg, 2010, Firth et al., 2010, Gan et al., 2010, Herranz et al., 2010, Li et al., 2010, Li et al., 2010, Li et al., 2010, Lopes and Casares, 2010, Monk et al., 2010, Roignant and Treisman, 2010, Silies and Klämbt, 2010, Swaminathan et al., 2010, Althoff et al., 2009, Chau et al., 2009, Glavic et al., 2009, Kurusu et al., 2009, Kwon et al., 2009, McCleland et al., 2009, Ozdowski et al., 2009, Peng et al., 2009, Rahmani et al., 2009, Shyu et al., 2009, Yu et al., 2009, Zsindely et al., 2009, Benoit et al., 2008, Braid and Verheyen, 2008, Chang et al., 2008, Chau et al., 2008, Chen et al., 2008, Christensen et al., 2008.9.3, Franciscovich et al., 2008, Hsu et al., 2008, Mitchell et al., 2008, Miura et al., 2008, Nomie and Wharton, 2008, O'Farrell and Kylsten, 2008, Oktaba et al., 2008, Reynolds et al., 2008, Royou et al., 2008, Shimizu et al., 2008, Yang and Fan, 2008, Ambrus et al., 2007, Baker and Fuller, 2007, Buszczak et al., 2007, Calzone et al., 2007, Cheerambathur et al., 2007, Gawlinski et al., 2007, Gawlinski et al., 2007, Georlette et al., 2007, Griffiths et al., 2007, Hong et al., 2007, Hueber et al., 2007, Kadyrova et al., 2007, LaRocque et al., 2007, LaRocque et al., 2007, Lecuyer et al., 2007, Magalhaes et al., 2007, Matsuno et al., 2007, Minakhina et al., 2007, Ou et al., 2007, Ramachandran et al., 2007, Sandmann et al., 2007, Song et al., 2007, Sun and Deng, 2007, Tanaka-Matakatsu et al., 2007, Tyler and Baker, 2007, Bartolome and Charlesworth, 2006, Bello et al., 2006, D'Costa et al., 2006, Delcros et al., 2006, Jones et al., 2006, Jordan et al., 2006, Khurana et al., 2006, Mukherjee et al., 2006, Sugimura and Lilly, 2006, Vrailas and Moses, 2006, Baonza and Freeman, 2005, Firth and Baker, 2005, Greene et al., 2005, Loop et al., 2004, Stanyon et al., 2004, Huang and Raff, 2002)
    chr2R:18312808..18312907
    Name Synonyms
    CycB PRE/TRE element
    Cyclin B
    (Costa and Ohkura, 2019, Von Stetina et al., 2018, Yao et al., 2018, Cruz-Becerra et al., 2016, Kotov et al., 2016, Buckalew et al., 2015, Défachelles et al., 2015, Derive et al., 2015, Maiato et al., 2015, Afonso et al., 2014, Ayeni et al., 2014, Hainline et al., 2014, Karandikar et al., 2014, Kuang et al., 2014, Li et al., 2014, Rubin et al., 2014, Sitaram et al., 2014, Wong et al., 2014, Zielke et al., 2014, Conde et al., 2013, Lu et al., 2013, Pankotai et al., 2013, Whitfield et al., 2013, Zhou and Luo, 2013, Zoranovic et al., 2013, Deshpande et al., 2012, Kanakousaki and Gibson, 2012, Lim et al., 2012, Loh et al., 2012, Ruggiero et al., 2012, Miles et al., 2011, Moon et al., 2011, Poulton et al., 2011, Rangone et al., 2011, Beam and Moberg, 2010, Firth et al., 2010, Günesdogan et al., 2010, Li et al., 2010, Li et al., 2010, Monk et al., 2010, Nishiyama et al., 2010, Papoulas et al., 2010, Althoff et al., 2009, Chau et al., 2009, Glavic et al., 2009, Ho et al., 2009, Martins et al., 2009, Peng et al., 2009, Rahmani et al., 2009, Chang et al., 2008, Colombié et al., 2008, Lee et al., 2008, Miura et al., 2008, Narbonne-Reveau et al., 2008, Pauli et al., 2008, Somma et al., 2008, Von Stetina et al., 2008, Zielke et al., 2008, Cheerambathur et al., 2007, Chien-Hsiang et al., 2007, Firth and Baker, 2007, Garcia et al., 2007, Gregory et al., 2007, Jiang et al., 2007, Kadyrova et al., 2007, Kaplow et al., 2007, Manak et al., 2007, Matsuno et al., 2007, Pfleger et al., 2007, Stitzel and Seydoux, 2007, Sun and Deng, 2007, Tyler and Baker, 2007, Vied et al., 2007, Khurana et al., 2006, Pines, 2006, Reber et al., 2006, Roignant et al., 2006, Silva, 2006, Silva et al., 2006, Vrailas and Moses, 2006, Zhang et al., 2006, Clarke et al., 2005, Harbison et al., 2005, Jager et al., 2005, Mandal et al., 2005, Savvidou et al., 2005, Yang et al., 2005, Furukawa et al., 2003, Leismann and Lehner, 2003, Schubiger et al., 1997)
    cyclin B
    (Eichler et al., 2020, Otsuki and Brand, 2020, Liao et al., 2019, Wang et al., 2018, Liu and Grosshans, 2017, Eichhorn et al., 2016, Gupte, 2015, Blanchard et al., 2014, Pushpavalli et al., 2014, Cui et al., 2013, Kanda et al., 2013, Wang et al., 2013, Althoff et al., 2012, Batiha and Swan, 2012, Mohd-Sarip et al., 2012, Andersen et al., 2010, Li et al., 2010, Lopes and Casares, 2010, Oliveira et al., 2010, Schneiderman et al., 2010, Anderson et al., 2009, Baldinger and Gossen, 2009, Benoit et al., 2009, Chen et al., 2009, Kwon et al., 2009, Lim et al., 2009, Rangan et al., 2009, Schaaf et al., 2009, Shyu et al., 2009, Ueishi et al., 2009, Zsindely et al., 2009, Braid and Verheyen, 2008, Chia et al., 2008, McCleland and O'Farrell, 2008, Rangan et al., 2008, Rencus-Lazar et al., 2008, Royou et al., 2008, Shields et al., 2008, Shimizu et al., 2008, Yang and Fan, 2008, Basto et al., 2007, Brunk et al., 2007, Buffin et al., 2007, Gregory et al., 2007, Huang et al., 2007, Huang et al., 2007, Kaplow et al., 2007, Ou et al., 2007, Pal et al., 2007, Pesin and Orr-Weaver, 2007, Rickmyre et al., 2007, Roignant et al., 2007, Sakaguchi and Steward, 2007, Sakaguchi and Steward, 2007, Swan and Schüpbach, 2007, Tadros et al., 2007, Vardy and Orr-Weaver, 2007, Williams et al., 2007, Delcros et al., 2006, Horner et al., 2006, Hyun et al., 2006, Yang and Fan, 2006, Benoit et al., 2005, Crest and Schubiger, 2005, Cullen et al., 2005, Findlay et al., 2005, Frolov et al., 2005, Lopes et al., 2005, May et al., 2005, Onischenko et al., 2005, Pearson et al., 2005, Douglas and Haddad, 2003, Echard and O'Farrell, 2003, Fung et al., 2003, Hickson et al., 2003, Leatherman and Jongens, 2003, Lee and Orr-Weaver, 2003, Huang and Raff, 2002, Mihaylov et al., 2002, Nieduszynski et al., 2002, Wickens et al., 2002, Deng and Lin, 2001, Mahowald, 2001, O'Farrell, 2001, Jaklevic et al., 2000, Carmena et al., 1998, Harper and Elledge, 1998, Secombe et al., 1998, Su and O'Farrell, 1997, Brown et al., 1996)
    Secondary FlyBase IDs
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (711)