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Gene Dmel\Hr46

General Information
Symbol	Dmel\Hr46	Species	D. melanogaster
Name	Hormone receptor-like in 46	Annotation symbol	CG33183
Feature type	protein_coding_gene	FlyBase ID	FBgn0000448
Gene Model Status	Current	Stock availability	35 publicly available
Also Known As	DHR3, NR1F4, HR3
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	46F5-46F7	Sequence location	2R:6,091,617..6,124,853 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Hormone receptor-like in 46 is referred to in FlyBase by the symbol Dmel\Hr46 (CG33183, FBgn0000448). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: RNA polymerase II regulatory region sequence-specific DNA binding; protein binding; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity. There is experimental evidence that it is involved in the biological process: negative regulation of transcription from RNA polymerase II promoter; mushroom body development; positive regulation of transcription from RNA polymerase II promoter. 69 alleles are reported. The phenotypes of these alleles are annotated with: taenidial fold; mushroom body; presumptive embryonic/larval peripheral nervous system; trichogen cell; wing; adult head; puparium. It has 7 annotated transcripts and 7 annotated polypeptides. Protein features are: Nuclear hormone receptor, ligand-binding; Nuclear hormone receptor, ligand-binding, core; Steroid hormone receptor; Thyroid hormone receptor; Zinc finger, NHR/GATA-type; Zinc finger, nuclear hormone receptor-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 12-18 embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1623180_a_at completely aligns to an exonic region common to each of the 4 FlyBase-annotated transcript isoforms of Hr46. Gene sequence location is 2R:6091617..6124853.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 46F5-46F7   Limits computationally determined from genome sequence between P{PZ}Pfk06339&P{PZ}CCS03221 and P{lacW}Hr46k10308
Experimentally Determined Cytological Location
Cytogenetic map Notes References 46F5-46F6   (determined by in situ hybridisation)   (Spradling et al., 1999) 46F-46F     (Gibson et al., 1999) 46F5-46F6   46F5--7   (BDGP Project Members, 1994-1999) 46F-46F   (determined by in situ hybridisation)   (Koelle et al., 1992) 46C8-46F6   Complementation data from unspecified deficiency chromosomes.   (Goldstein et al., 1993)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Hr46 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	gene_with_stop_codon_read_through ; SO:0000697 Stop-codon suppression (UAA) postulated; FBrf0216884 Gene model reviewed during 5.44 Low-frequency RNA-Seq exon junction(s) not annotated. gene_with_dicistronic_mRNA ; SO:0000722 Gene model reviewed during 5.49 Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Hr46-RA FBtr0088368  NM_176123   6071   487 Hr46-RC FBtr0088366  NM_176121   6466   694 Hr46-RD FBtr0112799  NM_001103786   4233   456 Hr46-RE FBtr0302438  NM_176122   6385   874 Hr46-RF FBtr0306345  NM_001259307   7407   487 Hr46-RG FBtr0306346  NM_001259308   4170   372 Hr46-RH FBtr0330609  NM_001273925   6071   529
Additional Transcript Data & Comments
Reported size (kB)	9, 7, 5.5 (northern blot) (Koelle et al., 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Hr46-PA   FBpp0087456   55.3   487   6.58     NP_788303   AAF58826 Hr46-PC   FBpp0087454   76.6   694   5.34     NP_788301   AAO41453 Hr46-PD   FBpp0111711   52.0   456   6.51     NP_001097256   ABV53751 Hr46-PE   FBpp0291630   95.0   874   5.48     NP_788302   AAO41454 Hr46-PF   FBpp0297438   55.3   487   6.58     NP_001246236   AFH07991 Hr46-PG   FBpp0297439   42.4   372   4.87     NP_001246237   AFH07992 Hr46-PH   FBpp0303459   59.5   529   5.37     NP_001260854   AGB93387
Additional Polypeptide Data & Comments
Reported size (kDa)	487 (aa) (Koelle et al., 1992)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Nuclear hormone receptor, ligand-binding, core (IPR000536) Zinc finger, nuclear hormone receptor-type (IPR001628) Steroid hormone receptor (IPR001723) Nuclear hormone receptor, ligand-binding (IPR008946) Zinc finger, NHR/GATA-type (IPR013088) Thyroid hormone receptor (IPR001728)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE013599 AAF58826   AE013599 AAO41453   AE013599 AAO41454   AE013599 ABV53751   AC005469   AC005974   AE013599 AFH07991   AE013599 AFH07992   AE013599 AGB93387   AI110006   AI387277   AI389835   AI402811   AQ025932   AW940332   AW940812   AY094723 AAM11076   BE662219   BH614833   BI372476   BI374666   BI374864   BI375167   BT028778   BT030130   BT072972   BT120080   BT133118   CL526200   CO176929   CO179438   CO179458   CO180764   CO182608   CO185137   CO187113   CO189542   CO190955   CO266231   CO270685   CO271255   CO274278   CO276023   CO278043   CO279577   CO280294   CO282353   CO287817   CO287841   CO288334   CO288342   CO290027   CO295547   CO296738   CO305909   CO306293   CO308128   CO309583   CO311991   CO317952   CO318086   CO318621   CO319915   CO324196   CO325371   CO329795   CO341259   CO342236   CO344030   CZ469921   CZ471512   CZ471537   CZ472590   CZ476438   CZ476453   CZ476454   CZ478426   CZ482609   CZ483800   CZ483801   CZ487353   EC058128   EC058600   EC066712   EC067303   EC069583   EC070141   EC075193   EC080062   EC080394   EC081700   EC082327   EC084178   EC086149   EC087245   EC195935   EC196039   EC198322   EC198423   EC200966   EC205379   EC208916   EC214629   EC215566   EC215642   EC218134   EC227819   EC229095   EC229424   EC235967   EC236281   EC237951   EC242040   EC243210   EC245227   EC247833   EC251222   EC253539   EC257138   EL873480   EL873481   EL875426   EL875539   EL884349   EL884350   EL884466   EL884592   EL884593   GH962366   GH967182   GH979697   GH982048   M90806 AAA28461
UniProtKB/Swiss-Prot	P31396
UniProtKB/TrEMBL	Q8SXA4 A1Z858 A8DY91
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference first instar larval stage     (Koelle et al., 1992) third instar larval stage     (Koelle et al., 1992) in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) foregut primordium   (Fisher et al., 2012) hindgut proper primordium   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) posterior spiracle primordium   (Fisher et al., 2012) Comment:reported as posterior spiracle specific anlage embryonic stage 13 -- 16   embryonic dorsal epidermis   (Fisher et al., 2012) embryonic epipharynx   (Fisher et al., 2012) embryonic foregut   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic hypopharynx   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval posterior spiracle   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) adult stage   adult mushroom body | restricted   (Kobayashi et al., 2006) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage | mid     (Koelle et al., 1992) second instar larval stage     (Koelle et al., 1992) pupal stage     (Koelle et al., 1992)
Additional Descriptive Data
	Peaks of Hr46 expression are observed at midembryogenesis, during larval instars L1 and L2, at the end of L3 extending into the prepupal stage, and during pupal development. Timing suggests that transcription is ecdysone induced. The 9kb Hr46 transcript is observed only during pupal development. (Koelle et al., 1992) Hr46 expression was studied in staged third instar larvae and prepupae collected at two hour intervals. Hr46 transcripts can be detected at low levels in late third instar larvae (116-120hr after egg laying). Expression peaks in 2-4hr prepupae and declines significantly by 8hr after puparium formation. In staged prepupal salivary glands, Hr46 transcripts are abundant in 0-2hr prepupal glands, decrease in 4hr glands, and are undetectable at later stages. Hr46 transcripts are rapidly induced by ecdysone. (Horner et al., 1995)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 14 -- 16   embryonic/larval gut   • nucleus (Lam et al., 1997) presumptive embryonic salivary gland   • nucleus (Lam et al., 1997) embryonic epidermis   • nucleus (Lam et al., 1997) late third instar larval stage   imaginal disc   • nucleus | faint (Lam et al., 1997) Comment:low levels embryonic/larval salivary gland | restricted   • nucleus (Lam et al., 1997) late third instar larval stage -- prepupal stage P2   salivary gland imaginal ring   • nucleus (Lam et al., 1997) embryonic/larval Malpighian tubule   • nucleus (Lam et al., 1997) prepupal stage P1   embryonic/larval salivary gland   • nucleus | faint (Lam et al., 1997) prepupal stage P1 -- P2   imaginal disc   • nucleus | intense (Lam et al., 1997) embryonic/larval fat body   • nucleus (Lam et al., 1997) peripodial epithelium   • nucleus (Lam et al., 1997) embryonic/larval midgut   • nucleus (Lam et al., 1997) embryonic/larval garland cell   • nucleus (Lam et al., 1997) embryonic/larval trachea   • nucleus (Lam et al., 1997) embryonic/larval lymph gland   • nucleus (Lam et al., 1997) ring gland   • nucleus (Lam et al., 1997) embryonic/larval hemocyte   • nucleus (Lam et al., 1997) embryonic/larval pericardial cell   • nucleus (Lam et al., 1997) prepupal stage P2   embryonic/larval salivary gland   • nucleus | intense (Lam et al., 1997) western blot Stage Tissue/Position (including subcellular localization) Reference prepupal stage P1   organism   (Lam et al., 1997) prepupal stage P3   organism   (Lam et al., 1997) Comment:4-6 hr APF
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Kageyama et al., 1997, Lam et al., 1997)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    8.1 Larval Midgut    0.8 Larval Hindgut    2.5 Larval Malpighian Tubules    13.2 Larval Fat Body    7.1 Larval Salivary Gland    4.2 Larval Trachea    2.575 Larval Carcass    1.55 Adult Head    12.8 Adult Eye    27.575 Adult Brain    90.9 Adult Thoracic-Abdominal Ganglion    37.8 Adult Crop    5.3 Adult Midgut    1.3 Adult Hindgut    2.6 Adult Malpighian Tubules    2.3 Adult Fat Body    12 Adult Salivary Gland    1.3 Adult Heart    3.1 Adult VirginFemale Spermatheca    9.2 Adult InseminatedFemale Spermatheca    8.4 Adult Ovary    1.1 Adult Testis    0.3 Adult Male Accessory Gland    3.3 Adult Carcass    5.5 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    8.1 Larval Midgut    0.8 Larval Hindgut    2.5 Larval Malpighian Tubules    13.2 Larval Fat Body    7.1 Larval Salivary Gland    4.2 Larval Trachea    2.575 Larval Carcass    1.55 Adult Head    12.8 Adult Eye    27.575 Adult Brain    90.9 Adult Thoracic-Abdominal Ganglion    37.8 Adult Crop    5.3 Adult Midgut    1.3 Adult Hindgut    2.6 Adult Malpighian Tubules    2.3 Adult Fat Body    12 Adult Salivary Gland    1.3 Adult Heart    3.1 Adult VirginFemale Spermatheca    9.2 Adult InseminatedFemale Spermatheca    8.4 Adult Ovary    1.1 Adult Testis    0.3 Adult Male Accessory Gland    3.3 Adult Carcass    5.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    8.1 Larval Midgut    0.8 Larval Hindgut    2.5 Larval Malpighian Tubules    13.2 Larval Fat Body    7.1 Larval Salivary Gland    4.2 Larval Trachea    2.575 Larval Carcass    1.55 Adult Head    12.8 Adult Eye    27.575 Adult Brain    90.9 Adult Thoracic-Abdominal Ganglion    37.8 Adult Crop    5.3 Adult Midgut    1.3 Adult Hindgut    2.6 Adult Malpighian Tubules    2.3 Adult Fat Body    12 Adult Salivary Gland    1.3 Adult Heart    3.1 Adult VirginFemale Spermatheca    9.2 Adult InseminatedFemale Spermatheca    8.4 Adult Ovary    1.1 Adult Testis    0.3 Adult Male Accessory Gland    3.3 Adult Carcass    5.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    8.1 Larval Midgut    0.8 Larval Hindgut    2.5 Larval Malpighian Tubules    13.2 Larval Fat Body    7.1 Larval Salivary Gland    4.2 Larval Trachea    2.575 Larval Carcass    1.55 Adult Head    12.8 Adult Eye    27.575 Adult Brain    90.9 Adult Thoracic-Abdominal Ganglion    37.8 Adult Crop    5.3 Adult Midgut    1.3 Adult Hindgut    2.6 Adult Malpighian Tubules    2.3 Adult Fat Body    12 Adult Salivary Gland    1.3 Adult Heart    3.1 Adult VirginFemale Spermatheca    9.2 Adult InseminatedFemale Spermatheca    8.4 Adult Ovary    1.1 Adult Testis    0.3 Adult Male Accessory Gland    3.3 Adult Carcass    5.5 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    8.1 Larval Midgut    0.8 Larval Hindgut    2.5 Larval Malpighian Tubules    13.2 Larval Fat Body    7.1 Larval Salivary Gland    4.2 Larval Trachea    2.575 Larval Carcass    1.55 Adult Head    12.8 Adult Eye    27.575 Adult Brain    90.9 Adult Thoracic-Abdominal Ganglion    37.8 Adult Crop    5.3 Adult Midgut    1.3 Adult Hindgut    2.6 Adult Malpighian Tubules    2.3 Adult Fat Body    12 Adult Salivary Gland    1.3 Adult Heart    3.1 Adult VirginFemale Spermatheca    9.2 Adult InseminatedFemale Spermatheca    8.4 Adult Ovary    1.1 Adult Testis    0.3 Adult Male Accessory Gland    3.3 Adult Carcass    5.5 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    8.1 Larval Midgut    0.8 Larval Hindgut    2.5 Larval Malpighian Tubules    13.2 Larval Fat Body    7.1 Larval Salivary Gland    4.2 Larval Trachea    2.575 Larval Carcass    1.55 Adult Head    12.8 Adult Eye    27.575 Adult Brain    90.9 Adult Thoracic-Abdominal Ganglion    37.8 Adult Crop    5.3 Adult Midgut    1.3 Adult Hindgut    2.6 Adult Malpighian Tubules    2.3 Adult Fat Body    12 Adult Salivary Gland    1.3 Adult Heart    3.1 Adult VirginFemale Spermatheca    9.2 Adult InseminatedFemale Spermatheca    8.4 Adult Ovary    1.1 Adult Testis    0.3 Adult Male Accessory Gland    3.3 Adult Carcass    5.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    8.1 Larval Midgut    0.8 Larval Hindgut    2.5 Larval Malpighian Tubules    13.2 Larval Fat Body    7.1 Larval Salivary Gland    4.2 Larval Trachea    2.575 Larval Carcass    1.55 Adult Head    12.8 Adult Eye    27.575 Adult Brain    90.9 Adult Thoracic-Abdominal Ganglion    37.8 Adult Crop    5.3 Adult Midgut    1.3 Adult Hindgut    2.6 Adult Malpighian Tubules    2.3 Adult Fat Body    12 Adult Salivary Gland    1.3 Adult Heart    3.1 Adult VirginFemale Spermatheca    9.2 Adult InseminatedFemale Spermatheca    8.4 Adult Ovary    1.1 Adult Testis    0.3 Adult Male Accessory Gland    3.3 Adult Carcass    5.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    8.1 Larval Midgut    0.8 Larval Hindgut    2.5 Larval Malpighian Tubules    13.2 Larval Fat Body    7.1 Larval Salivary Gland    4.2 Larval Trachea    2.575 Larval Carcass    1.55 Adult Head    12.8 Adult Eye    27.575 Adult Brain    90.9 Adult Thoracic-Abdominal Ganglion    37.8 Adult Crop    5.3 Adult Midgut    1.3 Adult Hindgut    2.6 Adult Malpighian Tubules    2.3 Adult Fat Body    12 Adult Salivary Gland    1.3 Adult Heart    3.1 Adult VirginFemale Spermatheca    9.2 Adult InseminatedFemale Spermatheca    8.4 Adult Ovary    1.1 Adult Testis    0.3 Adult Male Accessory Gland    3.3 Adult Carcass    5.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    12.8   NA  Eye    27.575   NA  Brain    90.9   8.1  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    37.8   NA  Crop    5.3   0.8  Midgut    1.3   2.5  Hindgut    2.6   13.2  Malpighian Tubules    2.3   7.1  Fat Body    no informative data   4.2  Salivary Gland    1.3   NA  Heart    3.1   2.575  Trachea    NA   NA  VirginFemale Spermatheca    9.2   NA  InseminatedFemale Spermatheca    8.4   NA  Ovary    1.1   NA  Testis    0.3   NA  Male Accessory Gland    3.3   1.55  Carcass    5.5 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    5 central nervous system, pupae P8    159 head, virgin 1-day female    11 head, virgin 4-day female    11 head, virgin 20-day female    5 head, mated 1-day female    12 head, mated 4-day female    9 head, mated 20-day female    6 head, mated 1-day male    17 head, mated 4-day male    19 head, mated 20-day male    12 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    117 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    32 fat body, pupae P8    47 carcass, larvae L3 wandering    11 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    5 central nervous system, pupae P8  (159) head, virgin 1-day female    11 head, virgin 4-day female    11 head, virgin 20-day female    5 head, mated 1-day female    12 head, mated 4-day female    9 head, mated 20-day female    6 head, mated 1-day male    17 head, mated 4-day male    19 head, mated 20-day male    12 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae  (117) digestive system, larvae L3 wandering    4 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae  (32) fat body, pupae P8  (47) carcass, larvae L3 wandering    11 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    5 central nervous system, pupae P8  (159) head, virgin 1-day female    11 head, virgin 4-day female    11 head, virgin 20-day female    5 head, mated 1-day female    12 head, mated 4-day female    9 head, mated 20-day female    6 head, mated 1-day male    17 head, mated 4-day male    19 head, mated 20-day male    12 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae  (117) digestive system, larvae L3 wandering    4 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    32 fat body, pupae P8    47 carcass, larvae L3 wandering    11 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    5 central nervous system, pupae P8    159 head, virgin 1-day female    11 head, virgin 4-day female    11 head, virgin 20-day female    5 head, mated 1-day female    12 head, mated 4-day female    9 head, mated 20-day female    6 head, mated 1-day male    17 head, mated 4-day male    19 head, mated 20-day male    12 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    117 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    32 fat body, pupae P8    47 carcass, larvae L3 wandering    11 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    5 central nervous system, pupae P8    159 head, virgin 1-day female    11 head, virgin 4-day female    11 head, virgin 20-day female    5 head, mated 1-day female    12 head, mated 4-day female    9 head, mated 20-day female    6 head, mated 1-day male    17 head, mated 4-day male    19 head, mated 20-day male    12 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    117 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    32 fat body, pupae P8    47 carcass, larvae L3 wandering    11 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    5 central nervous system, pupae P8  (159) head, virgin 1-day female    11 head, virgin 4-day female    11 head, virgin 20-day female    5 head, mated 1-day female    12 head, mated 4-day female    9 head, mated 20-day female    6 head, mated 1-day male    17 head, mated 4-day male    19 head, mated 20-day male    12 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae  (117) digestive system, larvae L3 wandering    4 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae  32 fat body, pupae P8  (47) carcass, larvae L3 wandering    11 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    5 central nervous system, pupae P8  (159) head, virgin 1-day female    11 head, virgin 4-day female    11 head, virgin 20-day female    5 head, mated 1-day female    12 head, mated 4-day female    9 head, mated 20-day female    6 head, mated 1-day male    17 head, mated 4-day male    19 head, mated 20-day male    12 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae  117 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    32 fat body, pupae P8    47 carcass, larvae L3 wandering    11 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    5 central nervous system, pupae P8    159 head, virgin 1-day female    11 head, virgin 4-day female    11 head, virgin 20-day female    5 head, mated 1-day female    12 head, mated 4-day female    9 head, mated 20-day female    6 head, mated 1-day male    17 head, mated 4-day male    19 head, mated 20-day male    12 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    117 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    0 fat body, larvae L3 wandering    1 fat body, white prepupae    32 fat body, pupae P8    47 carcass, larvae L3 wandering    11 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 12-18 embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    4 embryo 08-10hr    8 embryo 10-12hr    34 embryo 12-14hr    52 embryo 14-16hr    18 embryo 16-18hr    7 embryo 18-20hr    4 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    2 larva L2    10 larva L3 12hr old    2 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    10 white prepupae new    32 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    35 pupae 3d postWPP    30 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    4 embryo 08-10hr    8 embryo 10-12hr  (34) embryo 12-14hr  (52) embryo 14-16hr    18 embryo 16-18hr    7 embryo 18-20hr    4 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    2 larva L2    10 larva L3 12hr old    2 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    10 white prepupae new  (32) white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP  (35) pupae 3d postWPP  (30) pupae 4d postWPP    2 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    4 embryo 08-10hr    8 embryo 10-12hr    34 embryo 12-14hr    52 embryo 14-16hr    18 embryo 16-18hr    7 embryo 18-20hr    4 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    2 larva L2    10 larva L3 12hr old    2 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    10 white prepupae new    32 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    35 pupae 3d postWPP    30 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    4 embryo 08-10hr    8 embryo 10-12hr    34 embryo 12-14hr    52 embryo 14-16hr    18 embryo 16-18hr    7 embryo 18-20hr    4 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    2 larva L2    10 larva L3 12hr old    2 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    10 white prepupae new    32 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    35 pupae 3d postWPP    30 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    4 embryo 08-10hr    8 embryo 10-12hr    34 embryo 12-14hr    52 embryo 14-16hr    18 embryo 16-18hr    7 embryo 18-20hr    4 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    2 larva L2    10 larva L3 12hr old    2 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    10 white prepupae new    32 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    35 pupae 3d postWPP    30 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    4 embryo 08-10hr    8 embryo 10-12hr  (34) embryo 12-14hr  (52) embryo 14-16hr    18 embryo 16-18hr    7 embryo 18-20hr    4 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    2 larva L2    10 larva L3 12hr old    2 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    10 white prepupae new  32 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP  (35) pupae 3d postWPP  30 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    4 embryo 08-10hr    8 embryo 10-12hr    34 embryo 12-14hr    52 embryo 14-16hr    18 embryo 16-18hr    7 embryo 18-20hr    4 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    2 larva L2    10 larva L3 12hr old    2 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    10 white prepupae new    32 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    35 pupae 3d postWPP    30 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    4 embryo 08-10hr    8 embryo 10-12hr    34 embryo 12-14hr    52 embryo 14-16hr    18 embryo 16-18hr    7 embryo 18-20hr    4 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    2 larva L2    10 larva L3 12hr old    2 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    10 white prepupae new    32 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    35 pupae 3d postWPP    30 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    1 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    9 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    9 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    9 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    9 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    9 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    9 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    9 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    9 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_07
External Data & Images
Linkouts	BDGP expression data • GH21112 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000448 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG33183-RC FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Hr46 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4ap-md544 Hr46RA.Scer\UAS Hr46RB.Scer\UAS lethal, with Scer\GAL4pnr-MD237 Hr46GD4692 lethal (with Df(2R)12) Hr46TH21 Hr46TH29 Hr46TH31 lethal (with Df(2R)BSC281) Hr46TH29 lethal (with Df(2R)X1) Hr4612-6 Hr4614-4 Hr4614-14 Hr4622-12 Hr4622-24 Hr4622-35 Hr4624-28 Hr4625-74 Hr4630-45 Hr4651-10 Hr4666-20 Hr4691-9 Hr46k01017 Hr46k07705 Hr46k09242 Hr46k09838 Hr46k10308 Hr46k12305a Hr46TH29 lethal | embryonic stage Hr4622-35 Hr46G60S Hr46K243X Hr46R107G Hr46W284X lethal | polyphasic, with Hr4622-35 Hr46hs.PL lethal | polyphasic, with Hr46G60S Hr46hs.PL lethal | polyphasic, with Hr46hs.PL Hr4622-35 Hr46G60S lethal | recessive Hr4612-6 Hr4614-4 Hr4622-12 Hr4622-35 Hr4630-45 Hr4651-10 Hr4691-9 Hr46G60S Hr46k01017 Hr46k09838 Hr46k10308 Hr46R107G Hr46TH21 Hr46TH29 Hr46TH31 partially lethal - majority die | larval stage Hr46EY05451 Hr46EY10268 partially lethal - majority die | larval stage (with Hr46EY05451) Hr46EY10268 partially lethal - majority die | larval stage (with Hr46EY10268) Hr46EY05451 viable Hr46EY10268 viable, with Scer\GAL4pnr-MD237 Hr46GD7529 Sterility Allele female sterile Hr46EY10268 female sterile (with Hr46EY05451) Hr46EY10268 female sterile (with Hr46EY10268) Hr46EY05451 fertile Hr46EY10268 Other Phenotypes Allele decreased cell number | cell non-autonomous Hr46dsRNA.Scer\UAS Hr46EY05451 Hr46EY10268 decreased cell number | cell non-autonomous (with Hr46EY05451) Hr46EY10268 decreased cell number | cell non-autonomous (with Hr46EY10268) Hr46EY05451 developmental rate defective Hr46EY05451 Hr46EY10268 developmental rate defective (with Hr46EY05451) Hr46EY10268 developmental rate defective (with Hr46EY10268) Hr46EY05451 developmental rate defective | larval stage, with Scer\GAL4phm.PO Hr46NIG.12208R increased cell death | conditional, with Scer\GAL4GMR.PU Hr46RA.Scer\UAS Hr46RB.Scer\UAS large body | larval stage, with Scer\GAL4phm.PO Hr46NIG.12208R neuroanatomy defective, with Scer\GAL4ey-OK107 Hr46dsRNA.Scer\UAS visible, with Scer\GAL4pnr-MD237 Hr46GD7529 visible | heat sensitive Hr46hs.PL visible | somatic clone Hr4622-35 Hr46G60S Hr46R107G Phenotype manifest in Allele adult cuticle | somatic clone Hr4622-35 Hr46R107G adult head Hr4622-35 Hr46G60S embryonic/larval salivary gland | embryonic stage Hr4622-35 Hr46G60S eye disc | conditional, with Scer\GAL4GMR.PU Hr46RA.Scer\UAS Hr46RB.Scer\UAS longitudinal connective | posterior Hr46R107G macrochaeta & adult abdomen Hr46R107G macrochaeta & adult abdomen | somatic clone Hr4622-35 macrochaeta & adult head | somatic clone Hr4622-35 Hr46G60S macrochaeta & adult thorax | somatic clone Hr4622-35 Hr46G60S macrochaeta | somatic clone Hr4622-35 mushroom body, with Scer\GAL4ey-OK107 Hr46dsRNA.Scer\UAS presumptive embryonic/larval peripheral nervous system Hr4612-6 Hr4691-9 Hr46R107G puparium Hr4622-35 Hr46G60S taenidial fold Hr4622-35 Hr46G60S trachea & cuticle Hr4622-35 Hr46G60S trichogen cell, with Scer\GAL4pnr-MD237 Hr46GD7529 wing, with Scer\GAL4ap-md544 Hr46dsRNA.Scer\UAS Hr46ΔDBD.Scer\UAS wing | somatic clone Hr4622-35 Hr46G60S Hr46R107G wing hair | heat sensitive Hr46hs.PL
Classical Alleles ( 48 )
For All Classical Alleles Show
Allele of Hr46 Class Mutagen Stocks Known lesion Hr46k10308 P-element activity 2 -- Hr4612-6 ethyl methanesulfonate diepoxybutane gamma ray 1 -- Hr4614-14 ethyl methanesulfonate diepoxybutane gamma ray 1 -- Hr46BG00585 P-element activity 1 -- Hr46c05372 piggyBac transposase 1 -- Hr46CB-5644-3 1 -- Hr46d00299 P-element activity 1 -- Hr46d02054 P-element activity 1 -- Hr46d08848 P-element activity 1 -- Hr46d08874 P-element activity 1 -- Hr46e00549 piggyBac transposase 1 -- Hr46EY05451 P-element activity 1 -- Hr46EY10268 P-element activity 1 -- Hr46f00121 piggyBac transposase 1 -- Hr46f01327 piggyBac transposase 1 -- Hr46f04790 piggyBac transposase 1 -- Hr46k01017 P-element activity 1 -- Hr46k09242 P-element activity 1 -- Hr46k12305a 1 -- Hr46KG00850 P-element activity 1 -- Hr46KG02638 1 -- Hr46MI00362 1 -- Hr46MI02021 1 -- Hr46MI04877 1 -- Hr46NP0039 1 -- Hr46NP1234 1 -- Hr4614-4 ethyl methanesulfonate diepoxybutane gamma ray 0 -- Hr4622-12 ethyl methanesulfonate diepoxybutane gamma ray 0 -- Hr4622-24 ethyl methanesulfonate diepoxybutane gamma ray 0 -- Hr4622-35 ethyl methanesulfonate diepoxybutane gamma ray 0 -- Hr4624-28 ethyl methanesulfonate diepoxybutane gamma ray 0 -- Hr4625-74 ethyl methanesulfonate diepoxybutane gamma ray 0 -- Hr4630-45 ethyl methanesulfonate diepoxybutane gamma ray 0 -- Hr4651-10 ethyl methanesulfonate diepoxybutane gamma ray 0 -- Hr4666-20 ethyl methanesulfonate diepoxybutane gamma ray 0 -- Hr4691-9 ethyl methanesulfonate diepoxybutane gamma ray 0 -- Hr46d00344 P-element activity 0 -- Hr46G60S 0 Yes Hr46k07705 0 -- Hr46k09838 P-element activity 0 -- Hr46K243X ethyl methanesulfonate 0 -- Hr46R107G 0 Yes Hr46SH0829 0 -- Hr46TH21 ethyl methanesulfonate 0 -- Hr46TH29 ethyl methanesulfonate 0 -- Hr46TH31 ethyl methanesulfonate 0 -- Hr46unspecified 0 -- Hr46W284X ethyl methanesulfonate 0 --
Alleles Carried on Transgenic Constructs ( 21 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Hr46 Class Mutagen Stocks Known lesion Hr46hs.T:Scer\GAL4.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 2 Yes Hr46GD4692 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hr46GD7529 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hr46JF02542 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hr46JF02543 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hr46KK109115 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hr46T:Avic\GFP-SF,T:Zzzz\FLAG in vitro construct - coding region fusion 1 Yes Hr46dsRNA.Scer\UAS 0 Yes Hr46hs.PL in vitro construct - regulatory fusion 0 Yes Hr46hs.PW in vitro construct - regulatory fusion 0 Yes Hr46MtnA.PR in vitro construct - regulatory fusion 0 Yes Hr46MtnA.PW in vitro construct - regulatory fusion 0 Yes Hr46NIG.12208R in vitro construct - regulatory fusion in vitro construct - RNAi 0 -- Hr46RA.Scer\UAS in vitro construct - regulatory fusion 0 Yes Hr46RB.Scer\UAS in vitro construct - regulatory fusion 0 Yes Hr46Scer\UAS.cTa in vitro construct - regulatory fusion 0 Yes Hr46tCH321-23L02 in vitro construct - genomic fragment 0 Yes Hr46Δ105.MtnA in vitro construct - deletion 0 Yes Hr46Δ83.hs in vitro construct - deletion 0 Yes Hr46Δ83.MtnA in vitro construct - deletion 0 Yes Hr46ΔDBD.Scer\UAS in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Not disrupted in	Ab(2R)55-20 (Goldstein et al., 2001) Ab(2R)89-22 (Goldstein et al., 2001) Df(2R)27 (Otte et al., 2009) Df(2R)61-25 (Goldstein et al., 2001) Df(2R)71-81 (Goldstein et al., 2001) Df(2R)520 (Goldstein et al., 2001) Df(2R)BSC350 (Christensen and Cook, 2007.10.29) Df(2R)BSC595 (Otte et al., 2009) Df(2R)ED2076 (Otte et al., 2009) Df(2R)ED2098 (Otte et al., 2009) Df(2R)eve (Goldstein et al., 2001) Df(2R)X3 (Goldstein et al., 2001, Carney et al., 1997)
Disrupted in	Df(2R)12 (Lam et al., 1999, Spradling et al., 1999, BDGP Project Members, 1994-1999, Lam et al., 1997, Carney et al., 1997, Otte et al., 2009) Df(2R)64-10 (Goldstein et al., 2001) Df(2R)BSC281 (Christensen and Cook, 2007.5.8, Otte et al., 2009) Df(2R)stan2 (Goldstein et al., 2001) Df(2R)X1 (Goldstein et al., 1998, Spradling et al., 1999, Goldstein et al., 2001, BDGP Project Members, 1994-1999, Carney et al., 1997, Otte et al., 2009)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4692} NA P{GD7529} NA P{KK109115} NA P{TRiP.JF02542} NA P{TRiP.JF02543} NA P{UAS-Hr46.dsRNA} NA P{UAS-Hr46.RA} NA P{UAS-Hr46.RB} NA P{UAS-Hr46.T} NA P{UAS-Hr46.ΔDBD} NA P{UAS-Hr46-RNAi} NA GAL4 construct P{hs-GAL4-DHR3.LBD.HA} No characterization construct P{NIG.12208R} NA PBac{CH321-23L02} NA PBac{Hr46-GFP.FLAG} NA heat-shock construct P{hs-DHR3.L} NA P{hsDHR3} NA P{hs-GAL4-DHR3.LBD.HA} NA P{hsΔ83DHR3} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}Hr46EY05451 NA P{EPgy2}Hr46EY10268 NA P{XP}Hr46d00299 NA P{XP}Hr46d00344 NA P{XP}Hr46d02054 NA P{XP}Hr46d08848 NA P{XP}Hr46d08874 NA miscellaneous insertions Mi{MIC}Hr46MI00362 NA Mi{MIC}Hr46MI02021 NA Mi{MIC}Hr46MI04877 NA P{GT1}Hr46BG00585 NA P{RS3}Hr46CB-5644-3 NA P{SUPor-P}Hr46KG00850 NA P{SUPor-P}Hr46KG02638 NA PBac{PB}Hr46c05372 NA PBac{RB}Hr46e00549 NA PBac{WH}Hr46f00121 NA PBac{WH}Hr46f01327 NA PBac{WH}Hr46f04790 NA insertion of enhancer trap binary system P{GawB}Hr46NP0039 No P{GawB}Hr46NP1234 No insertion of enhancer trap P{lacW}Hr46k01017 No P{lacW}Hr46k09242 No P{lacW}Hr46k09838 No P{lacW}Hr46k10308 No P{lacW}Hr46k12305a No P{lacW}Hr46SH0829 No P{lacW}l(2)SH1218SH1218 No
Gene Ontology: Function, Process & Cellular Component ( 14 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function
CV term Evidence References
ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity inferred from direct assay (Kageyama et al., 1997) protein binding inferred from physical interaction with rig (Gates et al., 2004) RNA polymerase II regulatory region sequence-specific DNA binding inferred from direct assay (Lam et al., 1997)
Biological Process
CV term Evidence References
mushroom body development inferred from mutant phenotype (Kobayashi et al., 2006) negative regulation of transcription from RNA polymerase II promoter inferred from direct assay (Lam et al., 1997) positive regulation of transcription from RNA polymerase II promoter inferred from direct assay (Lam et al., 1997)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Kageyama et al., 1997, Lam et al., 1997)
Terms Based on Predictions or Assertions ( 9 terms )
Molecular Function
CV term Evidence References
ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity inferred from sequence or structural similarity with UniProtKB:Q8R1B8 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1993.7.1, Maglich et al., 2001) sequence-specific DNA binding transcription factor activity inferred from sequence or structural similarity with mouse Rora (FlyBase, 1992-) steroid hormone receptor activity inferred from electronic annotation with InterPro:IPR000536, InterPro:IPR001723, InterPro:IPR008946 (FlyBase Curators et al., 2004-) thyroid hormone receptor activity inferred from electronic annotation with InterPro:IPR001728 (FlyBase Curators et al., 2004-) zinc ion binding inferred from electronic annotation with InterPro:IPR001628, InterPro:IPR013088 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
metamorphosis traceable author statement (Thummel, 2001) regulation of development, heterochronic traceable author statement (Thummel, 2001) steroid hormone mediated signaling pathway inferred from electronic annotation with InterPro:IPR000536, InterPro:IPR008946 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity with UniProtKB:Q8R1B8 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1993.7.1)
Sequence Ontology: Class of Gene
	gene_with_dicistronic_mRNA (FlyBase Genome Annotators, 2012) gene_with_stop_codon_read_through (Jungreis et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Hr46 - Atx2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - CG10993 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - CG11107 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - CG11563 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - CG12163 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - CG12264 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - CG15735 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - CG6701 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - CG7878 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - Cg25C anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - Gapdh2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - LanB1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - Mp20 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - Pgi experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Hr46 - Prosβ7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - RpLP0 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - SC35 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - Syp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - YL-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - isoQC anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - kst anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - mRpL23 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - mRpL43 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - mod anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - nop5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Hr46 - shrb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Hr46 allele Gene References Hr46k09242 N (Rottgen et al., 1998) unspecified l(1)sc (Rottgen et al., 1998)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61895 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0000448 DroID - A comprehensive database of gene and protein interactions. • FBgn0000448 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36073
Orthologs
OrthoDB Orthologs (43) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6XD49V Dipteran inclusive ortholog search EOG6STSD0 Insect inclusive ortholog search EOG6HMH5N Arthropod inclusive ortholog search EOG6BRV21 Metazoa inclusive ortholog search EOG657J0X
Orthologs in Drosophila Species (EOG6XD49V)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Hr46     Drosophila simulans   Dsim\GD26001 Y   Drosophila sechellia   Dsec\GM20545 Y   Drosophila erecta   Dere\GG25225 Y   Drosophila yakuba   Dyak\GE21721 Y   Drosophila ananassae   Dana\GF12134 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA17350 Y   Drosophila persimilis   Dper\GL10487 Y   Drosophila willistoni   Dwil\GK18019 Y   Drosophila virilis   Dvir\GJ20161 Y   Drosophila mojavensis   Dmoj\GI20211 Y   Drosophila grimshawi   Dgri\GH20117 Y
Orthologs in non-Drosophila Dipterans (EOG6STSD0)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL015043   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL009588   Anopheles gambiae Malaria mosquito  Agam\AGAP009002   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ015542   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ018862
Orthologs in non-Dipteran Insects (EOG6HMH5N)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB47513   Apis mellifera Western honey bee  Amel\GB47512   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG006375   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH22019   Atta cephalotes Leafcutter ant  Acep\ACEP21705   Camponotus floridanus Florida carpenter ant  Cflo\CFLO14180   Camponotus floridanus Florida carpenter ant  Cflo\CFLO14181   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14038   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14037   Linepithema humile Argentine ant  Lhum\LH24981   Linepithema humile Argentine ant  Lhum\LH24982   Pogonomyrmex barbatus Red harvester ant  Pbar\PB17464   Pogonomyrmex barbatus Red harvester ant  Pbar\PB17461   Solenopsis invicta Red fire ant  Sinv\SINV21764   Solenopsis invicta Red fire ant  Sinv\SINV21767   Acyrthosiphon pisum Pea aphid  Apim\ACYPI56793   Bombyx mori Silkmoth  Bmor\BGIBMGA009688   Pediculus humanus Human body louse  Phum\PHUM053030   Tribolium castaneum Red flour beetle  Tcas\Hr46
Orthologs in non-Insect Arthropods (EOG6BRV21)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_311954   Ixodes scapularis Deer tick  Isca\ISCW008693
Orthologs in non-Arthropod Metazoa (EOG657J0X)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\nhr-23   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_021289gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_011576gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_018845gn   Danio rerio Zebrafish  Drer\rorab
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD26001 Drosophila sechellia Dsec\GM20545 Drosophila erecta Dere\GG25225 Drosophila yakuba Dyak\GE21721 Drosophila ananassae Dana\GF12134 Drosophila pseudoobscura pseudoobscura Dpse\GA17350 Drosophila persimilis Dper\GL10487 Drosophila willistoni Dwil\GK18019 Drosophila virilis Dvir\GJ20161 Drosophila mojavensis Dmoj\GI20211 Drosophila grimshawi Dgri\GH20117
Stocks & Reagents
Stocks Listed in FlyBase ( 35 )
Harvard	- P{XP}Hr46d00299 - P{XP}Hr46d02054 - P{XP}Hr46d08848 - P{XP}Hr46d08874 - PBac{PB}Hr46c05372 - PBac{RB}Hr46e00549 - PBac{WH}Hr46f00121 - PBac{WH}Hr46f01327 - PBac{WH}Hr46f04790
VDRC	v106837 P{KK109115}VIE-260B v12044 w1118; P{GD4692}v12044 v20157 w1118; P{GD7529}v20157
	More stocks available...
Genomic Clones ( 2 )
	BACR09J12 BACR48F07 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 102 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH21112 IP05237
Other clones	DPiM_GH21112 FI17818 IP15960 MIP09676 MIP15316
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH17764 GH22109 RE59445 RE62314 RE62549 RE62914
Other clones	19325 21119 29205 40554 64601 69271 73082 77287 81563 81656 83819 144324 146904 165316 165829 172453 190052 191244 214257.35 214449.37 281052 281580 bs31g11 CK01.11C.C10 CK01.11D.G7 CK01.29D.F7 CK01.30B.E4 CK01.54B.D11 CK01.63B.D4 CK01.76A.C8 CK01.78D.H2 CK01.84A.A8 CK01.86B.E12 CK01.86B.G12 CK01.93C.D5 CK01.109D.D12 CK01.128D.H12 CK01.145B.D6 EC05801 EC23201 EC29501 EK026316 EK044516 EK050235 EK051635 EK059408 EK059444 EK066902 EK066936 EK068250 EK068258 EK072915 EK099764 EK102706 EK113708 EK119447 EK128609 EK135414 EK145504 EK153639 EK160303 EK192947 EK196327 EK210655 EK214931 EK218449 EK227602 EK249658 EK250722 EK256804 EK269856 EK270268 EK273227 EK281407 EK293857 EP12129 EP14246 EP18542 GH09429 IP05337 IP05437 IP05537 IP15660 IP15760 IP15860 RP004225837 RP004248105 RP004471381 RP004526447
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0000448 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36073
Antibody Information
	polyclonal (Vorobyeva et al., 2012, Lam et al., 1997, Montagne et al., 2010, Vorobyeva et al., 2011)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Hr46 CG12208 (FlyBase Genome Annotators, 2002-2003) Source for merge of: Hr46 l(2)46CFi (Goldstein, 2004.3.15)
Additional comments	Annotations CG11823 and CG12208 merged as CG33183 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
Other Comments
	RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells. (Boutros et al., 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Hr46 is an essential regulator of the ftz-f1 midprepupal competence factor, providing a functional link between the late larval and prepupal responses to ecdysone. (Lam et al., 1999) Loss of Hr46 activity is not cell lethal. (Lam et al., 1999) Hr46 protein binds to the cis-regulatory region of the ftz-f1 gene, and these Hr46 protein binding sites are important for high level expression of ftz-f1. (Kageyama et al., 1997) Hr46 receptor represses the early genes activated by the late-larval ecdysone pulse and with the help of Eip75B, provides a temporal linkage between the two ecdysone responses by controlling the expression of ftz-f1. (White et al., 1997) Hr46 expression is temporally shifted throughout the onset of metamorphosis by Eip74EF mutations. (Fletcher and Thummel, 1995) Hr39 and Hr46 contribute to the regulatory hierarchy activated by the steroid hormone 20E at the onset of metamorphosis. (Horner et al., 1995) See Koelle, Seagraves and Hogness, PNAS 89:6167--6171. (Ayer et al., 1993) Complementation group identified in an EMS and DEB screen to isolate deficiencies that uncover Jra. (Goldstein et al., 1993) Structural similarity to the M.sexta early gene and the expression similarities to the known Drosophila early genes suggest that the Hr46 gene may also belong to the early class. (Koelle et al., 1992)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_176121 NM_176122 NM_176123 NM_001103786 NM_001259307 NM_001259308 NM_001273925 RefSeq (Proteins) • NP_788301 NP_788302 NP_788303 NP_001097256 NP_001246236 NP_001246237 NP_001260854 DDBJ / EMBL / GenBank • AAA28461 AAF58826 AAM11076 AAO41453 AAO41454 ABV53751 AC005469 AC005974 AFH07991 AFH07992 AGB93387 AI110006 AI389835 AQ025932 AW940332 AW940812 AY094723 BH614833 CL526200 CZ469921 CZ471512 CZ471537 CZ472590 CZ476438 CZ476453 CZ476454 CZ478426 CZ482609 CZ483800 CZ483801 CZ487353 M90806 Entrez Gene - A searchable database of RefSeq genes. • 36073 UniProtKB/Swiss-Prot • P31396 UniProtKB/TrEMBL • A1Z858 A8DY91 Q8SXA4
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Nuclear hormone receptor, ligand-binding, core (IPR000536) Zinc finger, nuclear hormone receptor-type (IPR001628) Steroid hormone receptor (IPR001723) Nuclear hormone receptor, ligand-binding (IPR008946) Zinc finger, NHR/GATA-type (IPR013088) Thyroid hormone receptor (IPR001728) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH21112
Linkouts
	BioGRID - A database of protein and genetic interactions • 61895 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0000448 DroID - A comprehensive database of gene and protein interactions. • FBgn0000448 DRSC - Results from RNAi screens. • FBgn0000448 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000448 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG33183-RC FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0000448 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36073 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /newgene/hr46-1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36073 modMine - Data generated by the modENCODE project. • FBgn0000448 REDfly - A database of transcriptional regulatory elements. • FBgn0000448
Synonyms & Secondary IDs ( 23 )
Reported As
Symbol Synonym	2.4 (Gibson et al., 1999) 12.6 (Currie and Sullivan, 1994) CG11823 (Fulton et al., 2001, ) CG12208 (Boutros et al., 2004, ) CG33183 (Chavoshi et al., 2010, Mosallanejad et al., 2010, Keleman et al., 2009.8.5, Montagne et al., 2010) D (Carney et al., 1997) DHR3 (Thummel, 2005, King-Jones et al., 2005, Kon et al., 2005, Li and White, 2003, Kumar et al., 2004, Gates et al., 2004, Schubiger et al., 2003, Bialecki et al., 2002, Thummel, 2001, Lafont, 2000, Sullivan and Thummel, 2003, Thummel and Chory, 2002, Beckstead et al., 2001, Robinow et al., 2001, Li and Bender, 2000, Pecasse et al., 2000, Schubiger and Truman, 2000, White et al., 1999, Lam et al., 1999, Sundaram et al., 1998, Richards et al., 1999, Dressel et al., 1999, Broadus et al., 1999, Schubiger and Truman, 1999, Lam et al., 1999, D'Avino and Thummel, 1998, Ueda et al., 1998, Kageyama et al., 1997, Carney et al., 1997, Thummel, 1997, Atkinson, 1994, Lam et al., 1997, Fletcher et al., 1997, White et al., 1997, Gilbert et al., 1997, Lam et al., 1997, Henrich and Brown, 1995, Fisk and Thummel, 1995, Horner et al., 1995, Huet et al., 1995, Fletcher and Thummel, 1995, Segraves, 1994, Jindra, 1994, Reinking et al., 2005, Palanker et al., 2006, King-Jones and Thummel, 2005, Ruaud et al., 2010, Monnier et al., 2007, Parvy and Montagne, 2007, Stabell et al., 2007, Rendina et al., 2010, Zeitouni et al., 2007, Johnston et al., 2011, Sun and Song, 2006, Bernardo et al., 2009, Kethidi et al., 2005, Beckstead et al., 2005, Ritter and Beckstead, 2010, Montagne et al., 2010, Rewitz et al., 2010, Francis et al., 2010, Vorobyeva et al., 2011, Cáceres et al., 2011, Dubrovsky et al., 2011, Kim et al., 2011, Akagi et al., 2013) Dhr3 (Goldstein et al., 1998, Thummel, 1995, Koelle et al., 1992, Palli et al., 1992, ) dHR3 (Laudet and Adelmant, 1995) DmRORα (Kim et al., 2011) HR3 (Nuclear Receptors Nomenclature Committee, 1999) Hr3 (Ayer et al., 1993) Hr46 (Christensen and Cook, 2007.5.8, Kobayashi et al., 2006, Chavoshi et al., 2010, Liebl et al., 2006, Zeitouni et al., 2007, Jungreis et al., 2011, Weake et al., 2011, Perkins et al., 2009.8.10, Venken et al., 2009, Otte et al., 2009, Spokony and White, 2012.11.14, FlyBase Genome Annotators, 2012) hr46 (Hong et al., 2006) l(2)46CFi   l(2)k09242 (Rottgen et al., 1998) NR1F4 (Nuclear Receptors Nomenclature Committee, 1999, King-Jones and Thummel, 2005) unnamed (Goldstein et al., 1993)
Name Synonym	Complementation group D (Goldstein et al., 2001, Carney et al., 1997) Hormone receptor 3 (Ayer et al., 1993, Montagne et al., 2010) hormone receptor 3 (Kethidi et al., 2005, Montagne et al., 2010, Cáceres et al., 2011) Hormone receptor-like 46 (Wang et al., 2006) Hormone receptor-like in 46 (Kobayashi et al., 2006, Zeitouni et al., 2007, Otte et al., 2009)
Secondary FlyBase IDs
	FBgn0019719 FBgn0026805 FBgn0033496
References ( 132 )
Generate a list of	All references relating to this gene ( 132 )
List References by type	Research paper  ( 73 ) Supplementary material  ( 9 ) Review  ( 13 ) Personal communication to FlyBase  ( 17 ) Abstract  ( 11 ) FlyBase analysis  ( 5 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 9 )
	Akagi et al., 2013, J. Mol. Biol. 425(1): 71--81 The Binding of Multiple Nuclear Receptors to a Single Regulatory Region Is Important for the Proper Expression of EDG84A in Drosophila melanogaster. [FBrf0220406] Fabian et al., 2012, Mol. Ecol. 21(19): 4748--4769 Genome-wide patterns of latitudinal differentiation among populations of Drosophila melanogaster from North America. [FBrf0219505] Vorobyeva et al., 2012, Nucleic Acids Res. 40(15): 7319--7331 SAYP and Brahma are important for 'repressive' and 'transient' Pol II pausing. [FBrf0219302] Cáceres et al., 2011, Genes Dev. 25(14): 1476--1485 Nitric oxide coordinates metabolism, growth, and development via the nuclear receptor E75. [FBrf0214403] Dubrovsky et al., 2011, J. Biol. Chem. 286(38): 33689--33700 The Drosophila FTZ-F1 Nuclear Receptor Mediates Juvenile Hormone Activation of E75A Gene Expression through an Intracellular Pathway. [FBrf0215576] Johnston et al., 2011, Mol. Cell 44(1): 51--61 Ecdysone- and NO-Mediated Gene Regulation by Competing EcR/Usp and E75A Nuclear Receptors during Drosophila Development. [FBrf0216342] Kim et al., 2011, Mol. Cell 44(5): 797--810 DNA Damage-Induced RORα Is Crucial for p53 Stabilization and Increased Apoptosis. [FBrf0216893] Vorobyeva et al., 2011, Cell Cycle 10(11): 1821--1827 SAYP interacts with DHR3 nuclear receptor and participates in ecdysone-dependent transcription regulation. [FBrf0213948] Weake et al., 2011, Genes Dev. 25(14): 1499--1509 Post-transcription initiation function of the ubiquitous SAGA complex in tissue-specific gene activation. [FBrf0214372] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...