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Gene Dmel\dor

General Information
Symbol	Dmel\dor	Species	D. melanogaster
Name	deep orange	Annotation symbol	CG3093
Feature type	protein_coding_gene	FlyBase ID	FBgn0000482
Gene Model Status	Current	Stock availability	16 publicly available
Also Known As	EG:171E4.1, Vps18
Genomic Location
Chromosome (arm)	X	Recombination map	1-0.4
Cytogenetic map	2B5-2B5	Sequence location	X:1,559,088..1,562,484 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene deep orange is referred to in FlyBase by the symbol Dmel\dor (CG3093, FBgn0000482). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: eye pigment granule organization; compound eye pigmentation; border follicle cell migration; cell division; protein targeting to lysosome; determination of adult lifespan; pteridine biosynthetic process; endosome organization; ommochrome biosynthetic process. 44 alleles are reported. The phenotypes of these alleles are annotated with: organ system; adult; somatic cell; female germline cyst; portion of tissue; organ system subdivision; nervous system; synapse; cytoplasmic part; external compound sense organ; adult segment. It has one annotated transcript and one annotated polypeptide. Protein features are: Clathrin, heavy chain/VPS, 7-fold repeat; Pep3/Vps18/deep orange; Zinc finger, RING-type; Zinc finger, RING/FYVE/PHD-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult salivary gland, adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1623139_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of dor. Gene sequence location is X:1559088..1562484.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	dor: deep orange dor mutants affect a number of developmental processes; severity of effect increases with increasing developmental temperature. Eye color orange, shade depending on allele and temperature. dor reduces eye pigmentation in combination with either cn or v or with bw, indicating reduction in both drosopterins and xanthommatin. Biochemical analyses show xanthommatin and five drosopterins to be reduced to different degrees in dor; levels, but not relative proportions, change according to temperature of development (Counce, 1957, Experientia 13: 354; Puckett and Petty, 1980, Biochem. Genet. 18: 1221-28). Reciprocal transplantation experiments show that eye color is autonomous (Hadorn and Counce). dor females produce no progeny in crosses to dor males at 25, although some allelic combinations able to produce progeny at 18; and a few dor/+ daughters are produced in crosses to + males. Germ-line clones homozygous for the lethal allele dor28 produce collapsed eggs (Perriman, Egstrom, and Mahowald, 1989, Genetics 121: 333-52). The lethal embryos produced by dor mothers reach gastrulation or beyond (Hildreth and Lucchesi, 1967, Dev. Biol. 15: 536-52; Counce, 1969, DIS 44: 101-82). Maternal effect shown to be germ line autonomous by both ovarian (Garen and Gehring, 1972, Proc. Nat. Acad. Sci. USA 69: 2982-85) and pole-cell transplantation (Marsh, van Deusen, Wieschaus, and Gehring, 1977, Dev. Biol. 56: 195-99). Maternal lethal effect rescuable by injections into preblastoderm embryos of cytoplasm from unfertilized eggs of normal females (Garen and Gehring, 1972); dor+ substance present during early stages of vitellogenesis but not detected in yolk of cellular blastoderm embryos (Marsh et al., 1977). Abnormalities of dor cells in culture eliminated by extracts of normal post- but not pregastrulation embryos (Kuroda, 1977, Dev. Growth Differ. 19: 57-66). dor males show variable extents of gonadal dysgenesis depending on culture conditions and genotypic background; abnormalities range from failure of testes to attach to genital ducts to failure of one attached testis to elongate (Lucchesi, Counce, and Hildreth, 1968, J. Exp. Zool. 168: 437-50). Viability and longevity of dor homozygotes and hemizygotes variably reduced depending on allele and temperature; dorl larvae develop melanotic pseudo-tumors (Stark, 1918, J. Exp. Zool. 27: 509-29; Oftedal, 1953, Z. Indukt. Abstamm. Vererbungsl. 85: 408-22) and midgut occlusion (Russell, 1940, J. Exp. Zool. 84: 363-79), dying in late third instar (Bischoff and Lucchesi, 1971). dor/dorl lethal at 29 (Belyaeva, Aizenzon, Semeshin, Kiss, Koczka, Baritcheva, Gorelova, and Zhimulev, 1980, Chromosoma 81: 281-306). dor in combination with ry, ry2 (Lucchesi, 1968, Genetics 59: 37-44) and car (Nash, 1971, DIS 47: 73) causes lethality in pupal stage. Recovery of gynandromorphs with dor car male sectors less than in controls; bilateral gynandromorphs not observed, but distribution of male tissue resembles that of control (Grell, 1976). dor behaves as a semilethal in combination with pd and with cn bw (Lucchesi, 1968).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10	Controlled Vocabulary Terms vesicle-mediated transport References Vidal et al., 2010, J. Cell Sci. 123(20): 3457--3466 Du et al., 2011, PLoS ONE 6(9): e24168
FB2012_01	Sequence features BKN22242 HFA18765 References Williamson et al., 2010, J. Cell Biol. 189(5): 885--899
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 2B5-2B5   Limits computationally determined from genome sequence between P{EP}brEP1515 and P{EP}EP1444&P{EP}CG14818EP1190
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location	1-0.4
Left of (cM)	hfw (Aizenzon and Belyaeva, 1982)
Right of (cM)	br (Aizenzon and Belyaeva, 1982)
Notes	dor has been mapped to the right of su(wa) using a duplication that covers su(wa). (Green, 1958)
Gene Model & Products
Please see the GBrowse view of Dmel\dor for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.39 Gene model reviewed during 5.42
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) dor-RA FBtr0070269  NM_057938   3158   1002
Additional Transcript Data & Comments
Reported size (kB)	3.1 (northern blot) (Shestopal et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein dor-PA   FBpp0070259   115.3   1002   5.52     NP_477286   AAF45652
Additional Polypeptide Data & Comments
Reported size (kDa)	1002 (aa); 115.3 (kD predicted) (Shestopal et al., 1997) 115 (kD observed) (Sevrioukov et al., 1999)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, RING-type (IPR001841) Pep3/Vps18/deep orange (IPR007810) Clathrin, heavy chain/VPS, 7-fold repeat (IPR000547) Zinc finger, RING/FYVE/PHD-type (IPR013083)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202506   AA390515   AA391097   AA439289   AA440898   AA540534   AA816263   AA816871   AA978607   AE014298 AAF45652   AI064408   AI455721   AI456009   AI456499   AI511675   AI516214   AI518691   AI519911   AI531026   AI532069   AI532702   AI533486   AI542612   AI546648   AL021726 CAA16809   AW944354   AY061592 AAL29140   BG639521   BI164517   BI166222   BI166852   BI170650   BI238509   BI353005   BI355377   BI484759   BI484885   BI636546   BI637350   BI638138   BT044436   CO177492   CO188424   CO268328   CO271915   CO292069   CO315967   EC060136   EC082480   EC224052   EC241501   EC249780   X86683 CAA60382   X86684
UniProtKB/Swiss-Prot	Q24314
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Shestopal et al., 1997)
Additional Descriptive Data
	The dor transcript is detected in all developmental stages, from the embryo to the adult. (Shestopal et al., 1997)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Sevrioukov et al., 1999) larval stage     (Sevrioukov et al., 1999) pupal stage     (Sevrioukov et al., 1999) adult stage | female   ovary   (Sevrioukov et al., 1999)   (Sevrioukov et al., 1999) adult stage | male     (Sevrioukov et al., 1999)
Additional Descriptive Data
	dor protein colocalizes with internalized dextran beads for a limited time in endocytic compartments in third larval instar garland cells. dor protein partially colocalizes with hk protein and with internalized dextran beads to endosomes in Schneider2:Sev cells. hk protein is confined to the perimeter of the large endosomes while dor protein is found in the interior as well. (Sevrioukov et al., 1999)
Marker for
Subcellular Localization
CV Term	endosome (Sevrioukov et al., 1999)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0000482    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0000482 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-18 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0000482 expression level Developmental Stage   Expression Level embryo 00-02hr    1546 embryo 02-04hr    1564 embryo 04-06hr    1701 embryo 06-08hr    2174 embryo 08-10hr    1985 embryo 10-12hr    1797 embryo 12-14hr    1304 embryo 14-16hr    1547 embryo 16-18hr    1032 embryo 18-20hr    803 embryo 20-22hr    998 embryo 22-24hr    890 larva L1    716 larva L2    527 larva L3 12hr old    493 larva L3 puffstage 1-2    590 larva L3 puffstage 3-6    892 larva L3 puffstage 7-9    898 white prepupae new    847 white prepupae 12hr    1021 white prepupae 24hr    1132 pupae 2d postWPP    696 pupae 3d postWPP    511 pupae 4d postWPP    482 adult male 01day    584 adult male 05day    637 adult male 30day    653 adult female 01day    830 adult female 05day    977 adult female 30day    983 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1546) embryo 02-04hr  (1564) embryo 04-06hr  (1701) embryo 06-08hr  (2174) embryo 08-10hr  (1985) embryo 10-12hr  (1797) embryo 12-14hr  (1304) embryo 14-16hr  (1547) embryo 16-18hr  (1032) embryo 18-20hr  (803) embryo 20-22hr  (998) embryo 22-24hr  (890) larva L1  (716) larva L2  (527) larva L3 12hr old  (493) larva L3 puffstage 1-2  (590) larva L3 puffstage 3-6  (892) larva L3 puffstage 7-9  (898) white prepupae new  (847) white prepupae 12hr  (1021) white prepupae 24hr  (1132) pupae 2d postWPP  (696) pupae 3d postWPP  (511) pupae 4d postWPP  (482) adult male 01day  (584) adult male 05day  (637) adult male 30day  (653) adult female 01day  (830) adult female 05day  (977) adult female 30day  (983) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (1546) embryo 02-04hr  (1564) embryo 04-06hr  (1701) embryo 06-08hr  (2174) embryo 08-10hr  (1985) embryo 10-12hr  (1797) embryo 12-14hr    1304 embryo 14-16hr  (1547) embryo 16-18hr    1032 embryo 18-20hr    803 embryo 20-22hr    998 embryo 22-24hr    890 larva L1    716 larva L2    527 larva L3 12hr old    493 larva L3 puffstage 1-2    590 larva L3 puffstage 3-6    892 larva L3 puffstage 7-9    898 white prepupae new    847 white prepupae 12hr    1021 white prepupae 24hr    1132 pupae 2d postWPP    696 pupae 3d postWPP    511 pupae 4d postWPP    482 adult male 01day    584 adult male 05day    637 adult male 30day    653 adult female 01day    830 adult female 05day    977 adult female 30day    983 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1546 embryo 02-04hr    1564 embryo 04-06hr    1701 embryo 06-08hr    2174 embryo 08-10hr    1985 embryo 10-12hr    1797 embryo 12-14hr    1304 embryo 14-16hr    1547 embryo 16-18hr    1032 embryo 18-20hr    803 embryo 20-22hr    998 embryo 22-24hr    890 larva L1    716 larva L2    527 larva L3 12hr old    493 larva L3 puffstage 1-2    590 larva L3 puffstage 3-6    892 larva L3 puffstage 7-9    898 white prepupae new    847 white prepupae 12hr    1021 white prepupae 24hr    1132 pupae 2d postWPP    696 pupae 3d postWPP    511 pupae 4d postWPP    482 adult male 01day    584 adult male 05day    637 adult male 30day    653 adult female 01day    830 adult female 05day    977 adult female 30day    983 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1546 embryo 02-04hr    1564 embryo 04-06hr    1701 embryo 06-08hr    2174 embryo 08-10hr    1985 embryo 10-12hr    1797 embryo 12-14hr    1304 embryo 14-16hr    1547 embryo 16-18hr    1032 embryo 18-20hr    803 embryo 20-22hr    998 embryo 22-24hr    890 larva L1    716 larva L2    527 larva L3 12hr old    493 larva L3 puffstage 1-2    590 larva L3 puffstage 3-6    892 larva L3 puffstage 7-9    898 white prepupae new    847 white prepupae 12hr    1021 white prepupae 24hr    1132 pupae 2d postWPP    696 pupae 3d postWPP    511 pupae 4d postWPP    482 adult male 01day    584 adult male 05day    637 adult male 30day    653 adult female 01day    830 adult female 05day    977 adult female 30day    983 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0000482 expression level Developmental Stage   Expression Level embryo 00-02hr    1546 embryo 02-04hr    1564 embryo 04-06hr    1701 embryo 06-08hr    2174 embryo 08-10hr    1985 embryo 10-12hr    1797 embryo 12-14hr    1304 embryo 14-16hr    1547 embryo 16-18hr    1032 embryo 18-20hr    803 embryo 20-22hr    998 embryo 22-24hr    890 larva L1    716 larva L2    527 larva L3 12hr old    493 larva L3 puffstage 1-2    590 larva L3 puffstage 3-6    892 larva L3 puffstage 7-9    898 white prepupae new    847 white prepupae 12hr    1021 white prepupae 24hr    1132 pupae 2d postWPP    696 pupae 3d postWPP    511 pupae 4d postWPP    482 adult male 01day    584 adult male 05day    637 adult male 30day    653 adult female 01day    830 adult female 05day    977 adult female 30day    983 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1546) embryo 02-04hr  (1564) embryo 04-06hr  (1701) embryo 06-08hr  (2174) embryo 08-10hr  (1985) embryo 10-12hr  (1797) embryo 12-14hr  (1304) embryo 14-16hr  (1547) embryo 16-18hr  (1032) embryo 18-20hr  (803) embryo 20-22hr  (998) embryo 22-24hr  (890) larva L1  (716) larva L2  (527) larva L3 12hr old  (493) larva L3 puffstage 1-2  (590) larva L3 puffstage 3-6  (892) larva L3 puffstage 7-9  (898) white prepupae new  (847) white prepupae 12hr  (1021) white prepupae 24hr  (1132) pupae 2d postWPP  (696) pupae 3d postWPP  (511) pupae 4d postWPP  (482) adult male 01day  (584) adult male 05day  (637) adult male 30day  (653) adult female 01day  (830) adult female 05day  (977) adult female 30day  (983) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  1546 embryo 02-04hr  1564 embryo 04-06hr  1701 embryo 06-08hr  2174 embryo 08-10hr  1985 embryo 10-12hr  1797 embryo 12-14hr    1304 embryo 14-16hr  1547 embryo 16-18hr    1032 embryo 18-20hr    803 embryo 20-22hr    998 embryo 22-24hr    890 larva L1    716 larva L2    527 larva L3 12hr old    493 larva L3 puffstage 1-2    590 larva L3 puffstage 3-6    892 larva L3 puffstage 7-9    898 white prepupae new    847 white prepupae 12hr    1021 white prepupae 24hr    1132 pupae 2d postWPP    696 pupae 3d postWPP    511 pupae 4d postWPP    482 adult male 01day    584 adult male 05day    637 adult male 30day    653 adult female 01day    830 adult female 05day    977 adult female 30day    983 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1546 embryo 02-04hr    1564 embryo 04-06hr    1701 embryo 06-08hr    2174 embryo 08-10hr    1985 embryo 10-12hr    1797 embryo 12-14hr    1304 embryo 14-16hr    1547 embryo 16-18hr    1032 embryo 18-20hr    803 embryo 20-22hr    998 embryo 22-24hr    890 larva L1    716 larva L2    527 larva L3 12hr old    493 larva L3 puffstage 1-2    590 larva L3 puffstage 3-6    892 larva L3 puffstage 7-9    898 white prepupae new    847 white prepupae 12hr    1021 white prepupae 24hr    1132 pupae 2d postWPP    696 pupae 3d postWPP    511 pupae 4d postWPP    482 adult male 01day    584 adult male 05day    637 adult male 30day    653 adult female 01day    830 adult female 05day    977 adult female 30day    983 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1546 embryo 02-04hr    1564 embryo 04-06hr    1701 embryo 06-08hr    2174 embryo 08-10hr    1985 embryo 10-12hr    1797 embryo 12-14hr    1304 embryo 14-16hr    1547 embryo 16-18hr    1032 embryo 18-20hr    803 embryo 20-22hr    998 embryo 22-24hr    890 larva L1    716 larva L2    527 larva L3 12hr old    493 larva L3 puffstage 1-2    590 larva L3 puffstage 3-6    892 larva L3 puffstage 7-9    898 white prepupae new    847 white prepupae 12hr    1021 white prepupae 24hr    1132 pupae 2d postWPP    696 pupae 3d postWPP    511 pupae 4d postWPP    482 adult male 01day    584 adult male 05day    637 adult male 30day    653 adult female 01day    830 adult female 05day    977 adult female 30day    983 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0000482    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0000482 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult salivary gland, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0000482 expression level Tissue   Expression Level Larval Central Nervous System    99.55 Larval Midgut    51.6 Larval Hindgut    45.6 Larval Malpighian Tubules    91.1 Larval Fat Body    62.8 Larval Salivary Gland    145.3 Larval Trachea    68.875 Larval Carcass no informative data Adult Head    39.9 Adult Eye    60.125 Adult Brain    63.9 Adult Thoracic-Abdominal Ganglion    55.4 Adult Crop    53.1 Adult Midgut    46.9 Adult Hindgut    49.5 Adult Malpighian Tubules    43.5 Adult Fat Body no informative data Adult Salivary Gland    109.6 Adult Heart    62.175 Adult VirginFemale Spermatheca    33.4 Adult InseminatedFemale Spermatheca    36.4 Adult Ovary    57.6 Adult Testis no informative data Adult Male Accessory Gland    107.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    99.55 Larval Midgut    51.6 Larval Hindgut    45.6 Larval Malpighian Tubules    91.1 Larval Fat Body    62.8 Larval Salivary Gland    145.3 Larval Trachea    68.875 Larval Carcass no informative data Adult Head    39.9 Adult Eye    60.125 Adult Brain    63.9 Adult Thoracic-Abdominal Ganglion    55.4 Adult Crop    53.1 Adult Midgut    46.9 Adult Hindgut    49.5 Adult Malpighian Tubules    43.5 Adult Fat Body no informative data Adult Salivary Gland    109.6 Adult Heart    62.175 Adult VirginFemale Spermatheca    33.4 Adult InseminatedFemale Spermatheca    36.4 Adult Ovary    57.6 Adult Testis no informative data Adult Male Accessory Gland    107.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    99.55 Larval Midgut    51.6 Larval Hindgut    45.6 Larval Malpighian Tubules    91.1 Larval Fat Body    62.8 Larval Salivary Gland    145.3 Larval Trachea    68.875 Larval Carcass no informative data Adult Head    39.9 Adult Eye    60.125 Adult Brain    63.9 Adult Thoracic-Abdominal Ganglion    55.4 Adult Crop    53.1 Adult Midgut    46.9 Adult Hindgut    49.5 Adult Malpighian Tubules    43.5 Adult Fat Body no informative data Adult Salivary Gland    109.6 Adult Heart    62.175 Adult VirginFemale Spermatheca    33.4 Adult InseminatedFemale Spermatheca    36.4 Adult Ovary    57.6 Adult Testis no informative data Adult Male Accessory Gland    107.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    99.55 Larval Midgut    51.6 Larval Hindgut    45.6 Larval Malpighian Tubules    91.1 Larval Fat Body    62.8 Larval Salivary Gland    145.3 Larval Trachea    68.875 Larval Carcass no informative data Adult Head    39.9 Adult Eye    60.125 Adult Brain    63.9 Adult Thoracic-Abdominal Ganglion    55.4 Adult Crop    53.1 Adult Midgut    46.9 Adult Hindgut    49.5 Adult Malpighian Tubules    43.5 Adult Fat Body no informative data Adult Salivary Gland    109.6 Adult Heart    62.175 Adult VirginFemale Spermatheca    33.4 Adult InseminatedFemale Spermatheca    36.4 Adult Ovary    57.6 Adult Testis no informative data Adult Male Accessory Gland    107.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0000482 expression level Tissue   Expression Level Larval Central Nervous System    99.55 Larval Midgut    51.6 Larval Hindgut    45.6 Larval Malpighian Tubules    91.1 Larval Fat Body    62.8 Larval Salivary Gland    145.3 Larval Trachea    68.875 Larval Carcass no informative data Adult Head    39.9 Adult Eye    60.125 Adult Brain    63.9 Adult Thoracic-Abdominal Ganglion    55.4 Adult Crop    53.1 Adult Midgut    46.9 Adult Hindgut    49.5 Adult Malpighian Tubules    43.5 Adult Fat Body no informative data Adult Salivary Gland    109.6 Adult Heart    62.175 Adult VirginFemale Spermatheca    33.4 Adult InseminatedFemale Spermatheca    36.4 Adult Ovary    57.6 Adult Testis no informative data Adult Male Accessory Gland    107.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    99.55 Larval Midgut    51.6 Larval Hindgut    45.6 Larval Malpighian Tubules    91.1 Larval Fat Body    62.8 Larval Salivary Gland    145.3 Larval Trachea    68.875 Larval Carcass no informative data Adult Head    39.9 Adult Eye    60.125 Adult Brain    63.9 Adult Thoracic-Abdominal Ganglion    55.4 Adult Crop    53.1 Adult Midgut    46.9 Adult Hindgut    49.5 Adult Malpighian Tubules    43.5 Adult Fat Body no informative data Adult Salivary Gland    109.6 Adult Heart    62.175 Adult VirginFemale Spermatheca    33.4 Adult InseminatedFemale Spermatheca    36.4 Adult Ovary    57.6 Adult Testis no informative data Adult Male Accessory Gland    107.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    99.55 Larval Midgut    51.6 Larval Hindgut    45.6 Larval Malpighian Tubules    91.1 Larval Fat Body    62.8 Larval Salivary Gland    145.3 Larval Trachea    68.875 Larval Carcass no informative data Adult Head    39.9 Adult Eye    60.125 Adult Brain    63.9 Adult Thoracic-Abdominal Ganglion    55.4 Adult Crop    53.1 Adult Midgut    46.9 Adult Hindgut    49.5 Adult Malpighian Tubules    43.5 Adult Fat Body no informative data Adult Salivary Gland    109.6 Adult Heart    62.175 Adult VirginFemale Spermatheca    33.4 Adult InseminatedFemale Spermatheca    36.4 Adult Ovary    57.6 Adult Testis no informative data Adult Male Accessory Gland    107.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    99.55 Larval Midgut    51.6 Larval Hindgut    45.6 Larval Malpighian Tubules    91.1 Larval Fat Body    62.8 Larval Salivary Gland    145.3 Larval Trachea    68.875 Larval Carcass no informative data Adult Head    39.9 Adult Eye    60.125 Adult Brain    63.9 Adult Thoracic-Abdominal Ganglion    55.4 Adult Crop    53.1 Adult Midgut    46.9 Adult Hindgut    49.5 Adult Malpighian Tubules    43.5 Adult Fat Body no informative data Adult Salivary Gland    109.6 Adult Heart    62.175 Adult VirginFemale Spermatheca    33.4 Adult InseminatedFemale Spermatheca    36.4 Adult Ovary    57.6 Adult Testis no informative data Adult Male Accessory Gland    107.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass no informative data Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body no informative data Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis no informative data Adult Male Accessory Gland     Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    39.9   NA  Eye    60.125   NA  Brain    63.9   99.55  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    55.4   NA  Crop    53.1   51.6  Midgut    46.9   45.6  Hindgut    49.5   91.1  Malpighian Tubules    43.5   62.8  Fat Body    no informative data   145.3  Salivary Gland    109.6   NA  Heart    62.175   68.875  Trachea    NA   NA  VirginFemale Spermatheca    33.4   NA  InseminatedFemale Spermatheca    36.4   NA  Ovary    57.6   NA  Testis    no informative data   NA  Male Accessory Gland    107.8   no informative data  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_17
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000482 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3093-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal dor8 dor11 dor12 dor13 dor14 dor15 dor16 dor17 dor18 dor19 dor20 dor21 dor22 dor23 dor24 dor25 dor26 dor27 dor28 dorlt148 lethal | larval stage dor8 dor22 dor29 lethal | larval stage | recessive dor1 dor8 dor12 dor19 dor20 dor21 dor22 dor23 dor28 dorlt148 lethal | male limited | third instar larval stage dor8 dorC107 dorD185 lethal | prepupal stage dor22 lethal | pupal stage, with Scer\GAL4pnr-MD237 dorGD10124 lethal | pupal stage | heat sensitive dor1 lethal | recessive dor8 dor9 dor10 dor29 viable dor1 dor3 dor4 dor5 dor6 Sterility Allele female fertile | reduced dor4 male fertile dor4 dor5 male sterile dor3 dor6 dor9 dor10 sterile dor8 Other Phenotypes Allele cell death defective | oogenesis stage S14 dor4 eye color defective dor1 dor3 dor4 dor5 dor6 dor8 dor9 dor10 dorC107 dorunspecified eye color defective | recessive | somatic clone dor8 melanotic mass phenotype dor8 dor9 dor10 short lived | female dor1 short lived | male dor1 dor4 visible | cold sensitive dor1 dor20 dor23 visible | heat sensitive dor1 dor20 dor23 visible | somatic clone dor8 Phenotype manifest in Allele adult foregut dor8 adult hindgut dor8 adult midgut dor8 border follicle cell, with Scer\GAL4slbo.2.6 dor1-1002.Scer\UAS bouton dor4 egg | germline clone dor28 egg chamber | oogenesis stage S14 dor4 eye dor1 dor4 eye | heat sensitive dor1 dor20 dor23 eye photoreceptor cell | somatic clone dor8 late endosome dor1 dor4 Malpighian tubule dor8 nurse cell | oogenesis stage S14 dor4 ovariole | germline clone dor28 pigment cell dor10 dor3 dor4 dor5 dor6 dor8 dor9 dorunspecified pigment cell | somatic clone dor8 polar follicle cell, with Scer\GAL4slbo.2.6 dor1-1002.Scer\UAS synapse dor4 thorax & macrochaeta | conditional cs dor1 dor20 dor23 wing dor1 dor20 dor23
Classical Alleles ( 36 )
For All Classical Alleles Show
Allele of dor Class Mutagen Stocks Known lesion dor8 spontaneous 4 Yes dor16 ethyl methanesulfonate 2 -- dor1 hypomorphic allele - genetic evidence X ray 2 Yes dor4 ethyl methanesulfonate 2 Yes dor84k 2 -- dor+ 1 -- dor10 spontaneous 0 -- dor11 ethyl methanesulfonate 0 -- dor12 ethyl methanesulfonate 0 -- dor13 ethyl methanesulfonate 0 -- dor14 ethyl methanesulfonate 0 -- dor15 ethyl methanesulfonate 0 -- dor17 ethyl methanesulfonate 0 -- dor18 ethyl methanesulfonate 0 -- dor19 ethyl methanesulfonate 0 -- dor20 ethyl methanesulfonate 0 -- dor21 ethyl methanesulfonate 0 -- dor22 ethyl methanesulfonate 0 -- dor23 ethyl methanesulfonate 0 -- dor24 ethyl methanesulfonate X ray 0 -- dor25 X ray 0 -- dor26 X ray 0 -- dor27 X ray 0 -- dor28 ethyl methanesulfonate 0 -- dor29 MR P-element activity 0 Yes dor2 0 -- dor3 spontaneous 0 -- dor5 ethyl methanesulfonate 0 -- dor6 ethyl methanesulfonate 0 -- dor7 ethyl methanesulfonate 0 -- dor9 X ray 0 -- dorC107 ethyl methanesulfonate 0 Yes dorD185 ethyl methanesulfonate 0 Yes dorlt148 ethyl methanesulfonate 0 -- dorSt479 0 -- dorunspecified 0 --
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of dor Class Mutagen Stocks Known lesion dorGD10124 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes dorKK102176 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes dor1-1002.Scer\UAS in vitro construct - regulatory fusion 0 Yes dor1-802.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes dor400-1002.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes dortSWI1 in vitro construct - genomic fragment 0 Yes dortSWI3 in vitro construct - genomic fragment 0 Yes dorΔ401-800.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Duplicated in	Dp(1;3)DC029 (Popodi et al., 2010-) Dp(1;3)DC104 (Popodi et al., 2010-) Dp(1;f)1337 (Rayle and Hoar, 1969, Hawley et al., 1992) Dp(1;f)1337D (Hawley et al., 1992) Dp(1;f)R (Eeken et al., 1985) Dp(1;Y)1E (Narayanan et al., 2000, Sriram et al., 2003, Masterson, 1968) Dp(1;Y)2E (Masterson, 1968, Aizenzon and Belyaeva, 1982, Kramers et al., 1983, Kramers et al., 1991) Dp(1;Y)2E-C (Rayle and Hoar, 1969) Dp(1;Y)Sz280 (Belyaeva et al., 1987) Dp(1;Y)y221T (Belyaeva et al., 1980) Dp(1;Y)y240T (Belyaeva et al., 1980) Dp(1;Y)y253T (Belyaeva et al., 1980, Aizenzon and Belyaeva, 1982) Dp(1;Y)y267g19.1 (Belyaeva et al., 1980, Lefevre, 1981, Aizenzon and Belyaeva, 1982, Eeken et al., 1985, Schalet, 1986, Pokholkova et al., 1993) Dp(1;Y)y267g24.2 (Belyaeva et al., 1980, Belyaeva et al., 1982, Aizenzon and Belyaeva, 1982, Protopopov et al., 1991, Pokholkova et al., 1993, Shestopal et al., 1997) Dp(1;Y)y2su(s)51c15 (Voelker et al., 1989) Ts(1Lt;4Lt)4C3 (Hawley et al., 1992) Ts(1Lt;4Lt)N264-12 (Hawley et al., 1992) Ts(1Lt;4Lt)wm5 (Hawley et al., 1992) Ts(1Lt;4Lt)wm258-18 (Hawley et al., 1992) Ts(1Lt;4Lt)wm258-21 (Hawley et al., 1992)
Disrupted in	Df(1)A52.3 (Prudhommeau and Proust, 1990) Df(1)A94 (Craymer and Roy, 1980, Kramers et al., 1983, Eeken et al., 1985, Kramers et al., 1991) Df(1)Exel8196 (Ryder, 2004.4.26, Chi et al., 2010) Df(1)H27.2 (Prudhommeau and Proust, 1990) Df(1)P154 (Belyaeva et al., 1987, Shestopal, 1997.2.6, Shestopal et al., 1997) Df(1)RA19 (Belyaeva et al., 1980, Belyaeva et al., 1982, Aizenzon and Belyaeva, 1982) Df(1)Sp(br-dor) (Schalet, 1986) Df(1)St472 (Belyaeva et al., 1982, Aizenzon and Belyaeva, 1982, Pokholkova et al., 1993, Shestopal et al., 1997) Dp(1;f)R (Chi et al., 2010) Dp(1;Y)y267g19.1 (Chi et al., 2010) Ts(1Lt;YLt)G20 (Belyaeva et al., 1987) Ts(1Lt;YSt)B131 (Belyaeva et al., 1987)
Not duplicated in	Dp(1;3)sta (Kramers et al., 1983, Kramers et al., 1991) Dp(1;3)wvco (Rayle and Hoar, 1969) Dp(1;f)3 (Rayle and Hoar, 1969) Dp(1;f)18 (Rayle and Hoar, 1969) Dp(1;f)52-LS (Hawley et al., 1992) Dp(1;f)101 (Rayle and Hoar, 1969, Belyaeva et al., 1980, Aizenzon and Belyaeva, 1982) Dp(1;f)856 (Hawley et al., 1992) Dp(1;f)1346 (Hawley et al., 1992) Dp(1;Y)59k9-4 (Kramers et al., 1983, Kramers et al., 1991) Dp(1;Y)Sz280 (Belyaeva et al., 1982, Aizenzon and Belyaeva, 1982, Protopopov et al., 1991) Dp(1;Y)y59b (Belyaeva et al., 1980, Aizenzon and Belyaeva, 1982) Dp(1;Y)y+mal+ (Eeken et al., 1985)
Not disrupted in	Df(1)403 (Kramers et al., 1991) Df(1)At127 (Aizenzon and Belyaeva, 1982) Df(1)br25 (Belyaeva et al., 1980, Belyaeva et al., 1987) Df(1)br-rv1 (Belyaeva et al., 1987) Df(1)cin-arth (Kramers et al., 1991) Df(1)HA18 (Schalet, 1986) Df(1)S39 (Belyaeva et al., 1980, Craymer and Roy, 1980, Belyaeva et al., 1982, Aizenzon and Belyaeva, 1982, Kramers et al., 1983, Belyaeva et al., 1987, Protopopov et al., 1991, Kramers et al., 1991, Shestopal et al., 1997) Df(1)Sp(J1a) (Schalet, 1986) Df(1)Sp(J1b) (Schalet, 1986) Df(1)Sp(J1c) (Schalet, 1986) Df(1)St490 (Belyaeva et al., 1987) Df(1)sta (Belyaeva et al., 1980, Aizenzon and Belyaeva, 1982, Kramers et al., 1983, Eeken et al., 1985, Belyaeva et al., 1987, Kramers et al., 1991) Df(1)svr (Kramers et al., 1991) Df(1)y74k24.1 (Eeken et al., 1985, Kramers et al., 1991) In(1)br103 (Belyaeva et al., 1987)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10124} NA P{KK102176} NA P{UAS-dor.1-802} NA P{UAS-dor.1-1002} NA P{UAS-dor.400-1002} NA P{UAS-dor.Δ401-800} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{}dor29 NA
Gene Ontology: Function, Process & Cellular Component ( 16 unique terms )
Terms Based on Experimental Evidence ( 12 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with car (assigned by UniProtKB) (Sevrioukov et al., 1999)
Biological Process
CV term Evidence References
border follicle cell migration inferred from mutant phenotype (Medioni and Noselli, 2005) cell division inferred from mutant phenotype (Chi et al., 2010) compound eye pigmentation inferred from mutant phenotype (Chi et al., 2010) determination of adult lifespan inferred from mutant phenotype (Simonsen et al., 2007) endosome organization inferred from mutant phenotype (Sriram et al., 2003) eye pigment granule organization inferred from mutant phenotype (assigned by UniProtKB) (Sevrioukov et al., 1999) ommochrome biosynthetic process inferred from mutant phenotype (Tearle, 1991) protein targeting to lysosome inferred from mutant phenotype (assigned by UniProtKB) (Sevrioukov et al., 1999) pteridine biosynthetic process inferred from mutant phenotype (Tearle, 1991)
Cellular Component
CV term Evidence References
lysosome inferred from direct assay (assigned by UniProtKB) (Sevrioukov et al., 1999) synapse inferred from mutant phenotype (Narayanan et al., 2000)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
zinc ion binding inferred from electronic annotation with InterPro:IPR001841 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
endosome to lysosome transport non-traceable author statement (Gonzalez-Gaitan, 2003) traceable author statement (Stimson and Ramaswami, 1999, Luzio et al., 2000, Narayanan and Ramaswami, 2001) eye pigment biosynthetic process traceable author statement (Perrimon et al., 1986) vesicle-mediated transport inferred from electronic annotation with InterPro:IPR000547 (FlyBase Curators et al., 2004-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions dor allele Gene References dor1 car (Sriram et al., 2003) lt (Warner et al., 1998) dor4 hk (Narayanan et al., 2000) dorunspecified w (Lloyd et al., 2002) unspecified bchs (Simonsen et al., 2007) Vps16A (Zucker et al., 2000)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 57669 DroID - A comprehensive database of gene and protein interactions. • FBgn0000482 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31118
Orthologs
Genome-wide drosophilid orthologs	Dana\GF22166 Dere\dor Dgri\GH17958 Dmoj\GI16496 Dper\GL14267 Dpse\GA15921 Dsec\GM19158 Dsim\dor Dvir\GJ16039 Dwil\GK25025 Dyak\GE16701
Curated drosophilid orthologs	Dere\dor (Ko et al., 2006, Ko et al., 2005.8.29) Dfun\dor   Dhyd\dor   Dkan\dor   Dmer\dor   Dmir\CG3093 (Thornton, 2005.12.20) Dore\dor (Ko et al., 2006, Ko et al., 2005.8.29) Dpar\dor   Dpse\GA15921   Dpse\dor   Drep\dor   Dsim\dor (Ko et al., 2006, Ko et al., 2005.8.29) Dtei\dor (Ko et al., 2006, Ko et al., 2005.8.29) Dvir\dor   Dyak\dor (Ko et al., 2006)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL004237-PA Aedes aegypti (mosquito) AGAP000983-PA Anopheles gambiae (mosquito) XP_396968.2 Apis mellifera (honey bee) XP_001945645.1 Acyrthosiphon pisum (pea aphid) At1g12470.1 Arabidopsis thaliana (wall cress) 24236 Batrachochytrium dendrobatidis (chytrid fungus) 115190 Branchiostoma floridae (lancelet) 117206 Branchiostoma floridae (lancelet) BGIBMGA007137-PA Bombyx mori (silk worm) ENSBTAP00000015368 Bos taurus (bovine) CAL0001382 Candida albicans (a form of yeast) CAL0006163 Candida albicans (a form of yeast) CAL0006258 Candida albicans (a form of yeast) CN02029 Caenorhabditis brenneri (small nematode) CBP22862 Caenorhabditis briggsae (nematode) CE32071 Caenorhabditis elegans (worm) ENSCAFP00000013817 Canis familiaris (dog) CAGL0M04653g Candida glabrata (haploid yeast) Chro.80110 Cryptosporidium hominis (obligate parasite) JA17982 Caenorhabditis japonica (nematode) cn03614 Cryptococcus neoformans (yeast-like fungus) cgd8_910 Cryptosporidium parvum (protozoan parasite) CPIJ005021 Culex pipens (mosquito) ENSCPOP00000016587 Cavia porcellus (guinea pig) RP36498 Caenorhabditis remanei (nematode) ENSCSAVP00000016437 Ciona savignyi (sedentary tunicate) 223579 Capitella spl (polychaete worm) DDB0234134 Dictyostelium discoideum (amoeba) DEHA2E19668g Debaryomyces Hansenii (yeast) ENSDARP00000094129 Danio rerio (zebra fish) ENSECAP00000014132 Equus caballus (horse) ENSGACP00000017148 Gymnopilus aculeatus (mushroom) ENSGALP00000013826 Gallus gallus (chicken) 109889 Hypsipyla robusta (cedar shoot borer) ENSP00000220509 Homo sapiens (human) ISCW004080-PA Ixodes scapularis (deer tick) KLLA0D17182g Kluyveromyces lactis (yeast) 214111 Lottia gigantea (owl limpet) LmjF27.0910 Leishmania major (virus) 33267 Monocosta brevicollis (choanoflagellate) ENSMODP00000000235 Monodelphis domestica (gray opossum) ENSMMUP00000019091 Macaca mulatta (rhesus monkey) ENSMUSP00000036915 Mus musculus (house mouse) 85536 Nematostella vectensis (starlet anemone) XP_001600368.1 Nasonia vitripennis (wasp) ENSORLP00000022583 Oryzias latipes (japanese killifish) LOC_Os08g08060.1 Oryza sativa (asian rice) 34701 Ostreococcus tauri (green alga) PF13_0053 Plasmodium falciparum (protozoal parasite) PHUM000194-PA Pediculus humanus (human body louse) PP07692 Pristionchus pacificus (nematode) 153664 Physcomitrella patens (moss) ENSPPYP00000007209 Pongo pygmaeus (orangutan) 72870 Phytophthora ramorum (sudden oak death pathogen) 140966 Phytophthora sojae (root rot pathogen) 730530 Populus trichocarpa (black cottonwood) ENSPTRP00000011838 Pan troglodytes (chimpanzee) ENSRNOP00000018717 Rattus norvegicus (norway rat) 4890346 Sorghum bicolor (sorghum) YLR148W Saccharomyces cerevisiae (budding yeast) Smp_142210 Schistosoma mansoni (trematode parasite) SPCC790.02 Schizosaccharomyces pombe (fission yeast) XP_001179427.1 Strongylocentrotus purpuratus (purple sea urchin) 55631 Trichoplax adhaerens (metazoa) TA14400 Theileria annulata (apicomplexan parasite) XP_974055.1 Tribolium castaneum (red flour beetle) Tc00.1047053511445.30 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000013896 Tetraodon nigroviridis (green spotted pufferfish) TP02_0075 Theileria parva (parasitic protozoan) ENSTRUP00000033149 Takifugu rubripes (pufferfish Takifugu) 3714.m00056 Tetrahymena thermophila (ciliate protozoa) ENSXETP00000046616 Xenopus tropicalis (frog) YALI0A19008g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB10937 NV17240 LH16157 ISCW004080 FBgn0234198 FBgn0226984 FBgn0203224 FBgn0085986 FBgn0174060 FBgn0075937 FBgn0151872 FBgn0139244 FBgn0125426 FBgn0086020 FBgn0099165 CPIJ005021 BGIBMGA007137 ACYPI007668 GB10262 AGAP000983 AAEL004237 PHUM429310 GLEAN_06604
Stocks & Reagents
Stocks Listed in FlyBase ( 16 )
Bloomington	28 dor8/FM6 751 dor1/C(1)DX, y1 f1 761 Dp(1;f)R, y+/y1 dor8 35 dor4/C(1)RM, y1 w1 f1
Kyoto	105686 dor8/FM6 106062 dor1/C(1)DX, y1 f1 106068 Dp(1;f)R, y+/y1 dor8 105769 dor16/C(1)DX, y1 f1/Dp(1;Y)1E 105692 dor4/C(1)RM, y1 w1 f1
VDRC	v107053 P{KK102176}VIE-260B v33733 w1118 P{GD10124}v33733 v33734 w1118 P{GD10124}v33734
	More stocks available...
Genomic Clones ( 1 )
	BACR33A08 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 47 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	SD04291
Other clones	FI09617
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH04821 GM21762 GM30568 LD01106 LD01985 LD09713 LD10054 LD12589 LD13816 LD20053 LD20251 LD32069 LD35374 LD35819 LD36538 LD38368 LD39755 LD42010 LD43155 RE04216 RE06283 RE07079 RE12173 RE34991 RE67910 RE68049 SD03433 SD05328 SD09028 SD09818 SD18258 SD19336 SD20353
Other clones	71249 160377 CK01.66A.F12 CK01.119B.C5 CK01.170D.C10 EK040112 EK053656 EK091011 EK105141 EK199119 EK263229 SD01881
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0000482 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31118
Antibody Information
	polyclonal (Sevrioukov et al., 1999, Pulipparacharuvil et al., 2005)
Other Information
Discoverer
	E.D. King.
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments	Mutants can be arranged in a linear sequence of increasing strengths; heterozygotes between different pairs of alleles show intermediate phenotypes; no evidence of interallelic complementation (Bischoff and Lucchesi, 1971). Recombinational mapping by Bischoff (1973) established the order illustrated in Lindsley and Zimm (1992) p.164.
Other Comments
	dsRNA has been made from templates generated with primers directed against this gene. (Rives et al., 2006) dsRNA made from templates generated with primers directed against dor that is transfected into S2 treated with Listeria monocytogenes reveals a vacuolar escape phenotype in which the bacterial protein listeriolysin O is not required. (Cheng et al., 2005) dor is required for normal labels of degradation of hh and ptc proteins in the wing disc, but hh signalling is unaffected by this. (Torroja et al., 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) dor mutations alter trafficking to lysosomes. (Sevrioukov et al., 1999) dor is part of a protein complex which includes car. (Sevrioukov et al., 1999) Molecular characterisation of dor. (Shestopal et al., 1997) Localisation of hfw, dor and br are clustered in the X chromosomes of taxonomically distant Drosophila species, suggesting an evolutionary significance of such an organisation. (Kokoza et al., 1992) dor has been located within a puff on the telomeric chromosome of D.funebris, D.virilis, D.hydei, D.repleta, D.mercatorum and D.paranaensis, within a puff on the distal part of the X chromosome of D.kanekoi and within a puff on the proximal portion of the X chromosome of D.pseudoobscura.pseudoobscura. (Kokoza et al., 1991) Lesions in dor reduce or eliminate pigmentation in the eyes and ocelli and block pigmentation of the fat body and tubules: dor is required for normal pigmentation of all four tissues. (Tearle, 1991) The dor locus is subject to T(1;2)dorvar7 position effect variegation. (Demakova et al., 1988) Lethal dor allele belongs to the class of ecdysone deficient mutations. (Biyasheva et al., 1985) dor mutants affect a number of developmental processes; severity of effect increases with increasing developmental temperature. Eye color orange, shade depending on allele and temperature. dor reduces eye pigmentation in combination with either cn or v or with bw, indicating reduction in both drosopterins and xanthommatin. Biochemical analyses show xanthommatin and five drosopterins to be reduced to different degrees in dor; levels, but not relative proportions, change according to temperature of development (Counce, 1957) (Puckett and Petty, 1980). Reciprocal transplantation experiments show that eye color is autonomous (Hadorn and Counce). dor females produce no progeny in crosses to dor males at 25oC, although some allelic combinations able to produce progeny at 18oC; and a few dor/+ daughters are produced in crosses to + males. Germ-line clones homozygous for the lethal allele dor28 produce collapsed eggs (Perriman, Egstrom and Mahowald, 1989). The lethal embryos produced by dor mothers reach gastrulation or beyond (Hildreth and Lucchesi, 1967; Counce, 1969). Maternal effect shown to be germ line autonomous by both ovarian (Garen and Gehring, 1972) and pole-cell transplantation (Marsh, van Deusen, Wieschaus and Gehring, 1977). Maternal lethal effect rescuable by injections into preblastoderm embryos of cytoplasm from unfertilized eggs of normal females (Garen and Gehring, 1972); dor+ substance present during early stages of vitellogenesis but not detected in yolk of cellular blastoderm embryos (Marsh et al., 1977). Abnormalities of dor cells in culture eliminated by extracts of normal post- but not pregastrulation embryos (Kuroda, 1977). dor males show variable extents of gonadal dysgenesis depending on culture conditions and genotypic background; abnormalities range from failure of testes to attach to genital ducts to failure of one attached testis to elongate (Lucchesi, Counce and Hildreth, 1968). Viability and longevity of dor homozygotes and hemizygotes variably reduced depending on allele and temperature; dor8 larvae develop melanotic pseudotumors (Stark, 1918; Oftedal, 1953) and midgut occlusion (Russell, 1940), dying in late third instar (Bischoff and Lucchesi, 1971). dor/dor8 lethal at 29oC (Belyaeva, Aizenzon, Semeshin, Kiss, Koczka, Baritcheva, Gorelova, and Zhimulev, 1980). dor in combination with ry, ry2 (Lucchesi, 1968) and car (Nash, 1971) causes lethality in pupal stage. Recovery of gynandromorphs with dor car male sectors less than in controls; bilateral gynandromorphs not observed, but distribution of male tissue resembles that of control (Grell, 1976). dor behaves as a semi-lethal in combination with pd and with cn bw (Lucchesi, 1968).
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057938 RefSeq (Proteins) • NP_477286 DDBJ / EMBL / GenBank • AA391097 AA440898 AAF45652 AAL29140 AI532702 AL009146 AL021726 AW944354 AY061592 CAA16809 CAA60382 X86683 X86684 Entrez Gene - A searchable database of RefSeq genes. • 31118 UniProtKB/Swiss-Prot • Q24314
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, RING-type (IPR001841) Pep3/Vps18/deep orange (IPR007810) Clathrin, heavy chain/VPS, 7-fold repeat (IPR000547) Zinc finger, RING/FYVE/PHD-type (IPR013083)
Linkouts
	BioGRID - A database of protein and genetic interactions • 57669 DroID - A comprehensive database of gene and protein interactions. • FBgn0000482 DRSC - Results from RNAi screens. • FBgn0000482 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000482 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3093-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0000482 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31118 InParanoid A subset of ortholog calls from InParanoid. • AAEL004237-PA Aedes aegypti (mosquito) AGAP000983-PA Anopheles gambiae (mosquito) XP_396968.2 Apis mellifera (honey bee) XP_001945645.1 Acyrthosiphon pisum (pea aphid) At1g12470.1 Arabidopsis thaliana (wall cress) 24236 Batrachochytrium dendrobatidis (chytrid fungus) 115190 Branchiostoma floridae (lancelet) 117206 Branchiostoma floridae (lancelet) BGIBMGA007137-PA Bombyx mori (silk worm) ENSBTAP00000015368 Bos taurus (bovine) CAL0001382 Candida albicans (a form of yeast) CAL0006163 Candida albicans (a form of yeast) CAL0006258 Candida albicans (a form of yeast) CN02029 Caenorhabditis brenneri (small nematode) CBP22862 Caenorhabditis briggsae (nematode) CE32071 Caenorhabditis elegans (worm) ENSCAFP00000013817 Canis familiaris (dog) CAGL0M04653g Candida glabrata (haploid yeast) Chro.80110 Cryptosporidium hominis (obligate parasite) JA17982 Caenorhabditis japonica (nematode) cn03614 Cryptococcus neoformans (yeast-like fungus) cgd8_910 Cryptosporidium parvum (protozoan parasite) CPIJ005021 Culex pipens (mosquito) ENSCPOP00000016587 Cavia porcellus (guinea pig) RP36498 Caenorhabditis remanei (nematode) ENSCSAVP00000016437 Ciona savignyi (sedentary tunicate) 223579 Capitella spl (polychaete worm) DDB0234134 Dictyostelium discoideum (amoeba) DEHA2E19668g Debaryomyces Hansenii (yeast) ENSDARP00000094129 Danio rerio (zebra fish) ENSECAP00000014132 Equus caballus (horse) ENSGACP00000017148 Gymnopilus aculeatus (mushroom) ENSGALP00000013826 Gallus gallus (chicken) 109889 Hypsipyla robusta (cedar shoot borer) ENSP00000220509 Homo sapiens (human) ISCW004080-PA Ixodes scapularis (deer tick) KLLA0D17182g Kluyveromyces lactis (yeast) 214111 Lottia gigantea (owl limpet) LmjF27.0910 Leishmania major (virus) 33267 Monocosta brevicollis (choanoflagellate) ENSMODP00000000235 Monodelphis domestica (gray opossum) ENSMMUP00000019091 Macaca mulatta (rhesus monkey) ENSMUSP00000036915 Mus musculus (house mouse) 85536 Nematostella vectensis (starlet anemone) XP_001600368.1 Nasonia vitripennis (wasp) ENSORLP00000022583 Oryzias latipes (japanese killifish) LOC_Os08g08060.1 Oryza sativa (asian rice) 34701 Ostreococcus tauri (green alga) PF13_0053 Plasmodium falciparum (protozoal parasite) PHUM000194-PA Pediculus humanus (human body louse) PP07692 Pristionchus pacificus (nematode) 153664 Physcomitrella patens (moss) ENSPPYP00000007209 Pongo pygmaeus (orangutan) 72870 Phytophthora ramorum (sudden oak death pathogen) 140966 Phytophthora sojae (root rot pathogen) 730530 Populus trichocarpa (black cottonwood) ENSPTRP00000011838 Pan troglodytes (chimpanzee) ENSRNOP00000018717 Rattus norvegicus (norway rat) 4890346 Sorghum bicolor (sorghum) YLR148W Saccharomyces cerevisiae (budding yeast) Smp_142210 Schistosoma mansoni (trematode parasite) SPCC790.02 Schizosaccharomyces pombe (fission yeast) XP_001179427.1 Strongylocentrotus purpuratus (purple sea urchin) 55631 Trichoplax adhaerens (metazoa) TA14400 Theileria annulata (apicomplexan parasite) XP_974055.1 Tribolium castaneum (red flour beetle) Tc00.1047053511445.30 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000013896 Tetraodon nigroviridis (green spotted pufferfish) TP02_0075 Theileria parva (parasitic protozoan) ENSTRUP00000033149 Takifugu rubripes (pufferfish Takifugu) 3714.m00056 Tetrahymena thermophila (ciliate protozoa) ENSXETP00000046616 Xenopus tropicalis (frog) YALI0A19008g Yarrowia lipolytica (nonconventional yeast) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31118 modMine - Data generated by the modENCODE project. • FBgn0000482 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB10937 NV17240 LH16157 ISCW004080 FBgn0234198 FBgn0226984 FBgn0203224 FBgn0085986 FBgn0174060 FBgn0075937 FBgn0151872 FBgn0139244 FBgn0125426 FBgn0086020 FBgn0099165 CPIJ005021 BGIBMGA007137 ACYPI007668 GB10262 AGAP000983 AAEL004237 PHUM429310 GLEAN_06604
Synonyms & Secondary IDs ( 14 )
Reported As
Symbol Synonym	CG3093 (Benos, 2000.12.13, Keleman et al., 2009.8.5, Vidal et al., 2010) Deep-orange (Gonzalez-Gaitan, 2003) dor (Falcon-Perez et al., 2007, Ko et al., 2006, Du et al., 2011, Simonsen et al., 2007, Sevrioukov et al., 2005, Burgess et al., 2008, Callejo et al., 2006, Wilkin et al., 2008, Pulipparacharuvil et al., 2005, Devergne et al., 2007, Strutt and Strutt, 2008, Akbar et al., 2009, Anaka et al., 2008, Soukup et al., 2009, Massarwa et al., 2009, Popodi et al., 2010-, Cherry and Perrimon, 2004, Bass et al., 2009, Chi et al., 2010) Dor (Gonzalez-Gaitan, 2003, Jaekel and Klein, 2006, Miura et al., 2008, Kanwar and Fortini, 2008, Kim et al., 2010, Vidal et al., 2010, Williamson et al., 2010) dor1 (Gateff, 1994) EG:171E4.1 (Benos et al., 2001, Benos, 2000.12.13, ) l(1)2Be   l(1)2Bw   l(1)7 (Gateff, 1994, ) l(1)76   unnamed (Georgel et al., 1991) Vps18 (Strutt and Strutt, 2008, Kim et al., 2010)
Name Synonym	deep orange (Falcon-Perez et al., 2007, Du et al., 2011, Simonsen et al., 2007, Wilkin et al., 2008, Pulipparacharuvil et al., 2005, Devergne et al., 2007, Strutt and Strutt, 2008, Anaka et al., 2008, Massarwa et al., 2009, Cherry and Perrimon, 2004, Bass et al., 2009, Chi et al., 2010) Deep orange (Rives et al., 2006, Nagaraj and Banerjee, 2009)
Secondary FlyBase IDs
References ( 165 )
Generate a list of	All references relating to this gene ( 165 )
List References by type	Research paper  ( 103 ) Supplementary material  ( 4 ) Review  ( 24 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 15 ) FlyBase analysis  ( 2 ) Computer file  ( 1 ) DNA/RNA sequence record  ( 5 ) Stock list  ( 1 ) Automatic genome annotation  ( 1 ) Book  ( 1 )
Recent research papers ( 5 )
	Du et al., 2011, PLoS ONE 6(9): e24168 In Vivo RNAi Screen Reveals Neddylation Genes as Novel Regulators of Hedgehog Signaling. [FBrf0215574] Chi et al., 2010, J. Biol. Chem. 285(28): 21817--21823 Disruption of lysosome function promotes tumor growth and metastasis in Drosophila. [FBrf0211186] Kim et al., 2010, J. Cell Biol. 188(5): 717--734 The novel endosomal membrane protein Ema interacts with the class C Vps-HOPS complex to promote endosomal maturation. [FBrf0210177] Vidal et al., 2010, J. Cell Sci. 123(20): 3457--3466 Negative regulation of Drosophila JAK-STAT signalling by endocytic trafficking. [FBrf0211993] Williamson et al., 2010, J. Cell Biol. 189(5): 885--899 A dual function of V0-ATPase a1 provides an endolysosomal degradation mechanism in Drosophila melanogaster photoreceptors. [FBrf0210877] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...