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Gene Dmel\E(bx)

General Information
Symbol	Dmel\E(bx)	Species	D. melanogaster
Name	Enhancer of bithorax	Annotation symbol	CG32346
Feature type	protein_coding_gene	FlyBase ID	FBgn0000541
Gene Model Status	Current	Stock availability	16 publicly available
Also Known As	Nurf301, NURF, CG17135, p301, dNURF, NURF-215, NURF-301
Genomic Location
Chromosome (arm)	3L	Recombination map	3-
Cytogenetic map	61B2-61B3	Sequence location	3L:233,926..246,912 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Enhancer of bithorax is referred to in FlyBase by the symbol Dmel\E(bx) (CG32346, FBgn0000541). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: ligand-dependent nuclear receptor binding. There is experimental evidence for 11 unique biological process terms, many of which group under: cellular component organization or biogenesis; organelle organization; chromosome organization; biological regulation; single-organism developmental process; cellular process; gene expression; cell cycle; cell fate commitment; prepupal development. 30 alleles are reported. The phenotypes of these alleles are annotated with: dendrite; polytene chromosome; mesothoracic tergum; melanotic mass. It has 9 annotated transcripts and 9 annotated polypeptides. Protein features are: AT hook, DNA-binding motif; Bromodomain; Bromodomain, conserved site; DDT domain; DDT domain superfamily; DDT domain, subgroup; Zinc finger, FYVE/PHD-type; Zinc finger, PHD-finger; Zinc finger, PHD-type; Zinc finger, PHD-type, conserved site; Zinc finger, RING/FYVE/PHD-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, larval salivary gland, larval trachea, adult ovary, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1625243_a_at completely aligns to an exonic region of only a subset of 3 of the 5 FlyBase-annotated transcript isoforms of E(bx). Gene sequence location is 3L:233926..246912.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	E(bx): Enhancer of bithorax Enhances expression of bx34e, bx3, and Ubx/+. Lethal homozygous. RK2.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03	Controlled Vocabulary Terms regulation of transcription from RNA polymerase II promoter
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 61B2-61B3   Limits computationally determined from genome sequence between P{PZ}trh10512 and P{EP}EP3104EP3104
Experimentally Determined Cytological Location
Cytogenetic map Notes References 61A-61A   (determined by in situ hybridisation)   (Berg and Spradling, 1991)
Experimentally Determined Recombination Data
Location	3-
Left of (cM)	R
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\E(bx) for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.45 Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters. Annotated transcripts do not represent all supported alternative splices within 5' UTR. Low-frequency RNA-seq exon junction(s) not annotated. Apparent introns in second long intron not annotated: probable artifact due to repetitive sequence.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) E(bx)-RA FBtr0072521  NM_167819   9020   2669 E(bx)-RB FBtr0072522  NM_167817   8960   2649 E(bx)-RC FBtr0072523  NM_206224   7834   2159 E(bx)-RE FBtr0301348  NM_001169833   8975   2669 E(bx)-RF FBtr0301349  NM_001169834   7362   2139 E(bx)-RG FBtr0332101  NM_001274258   8652   2163 E(bx)-RH FBtr0332102  NM_001274259   9296   2761 E(bx)-RI FBtr0332103  NM_001274260   8605   2668 E(bx)-RJ FBtr0332104  NM_001274261   8517   2669
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein E(bx)-PA   FBpp0072421   300.7   2669   8.68     NP_728507   AAN11431 E(bx)-PB   FBpp0072422   298.5   2649   8.66     NP_728505   AAF47361 E(bx)-PC   FBpp0089425   241.6   2159   9.56     NP_995946   AAS64922 E(bx)-PE   FBpp0290562   300.7   2669   8.68     NP_001163304   ACZ94576 E(bx)-PF   FBpp0290563   239.5   2139   9.54     NP_001163305   ACZ94577 E(bx)-PG   FBpp0304411   242.2   2163   9.58     NP_001261187   AGB93882 E(bx)-PH   FBpp0304412   311.4   2761   8.37     NP_001261188   AGB93883 E(bx)-PI   FBpp0304413   300.6   2668   8.71     NP_001261189   AGB93884 E(bx)-PJ   FBpp0304414   300.7   2669   8.68     NP_001261190   AGB93885
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • AT hook, DNA-binding motif (IPR017956) Bromodomain, conserved site (IPR018359) DDT domain, subgroup (IPR018500) DDT domain superfamily (IPR018501) Bromodomain (IPR001487) Zinc finger, PHD-type (IPR001965) DDT domain (IPR004022) Zinc finger, FYVE/PHD-type (IPR011011) Zinc finger, PHD-type, conserved site (IPR019786) Zinc finger, PHD-finger (IPR019787) Zinc finger, RING/FYVE/PHD-type (IPR013083)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA539876   AA950197   AE014296 AAF47361   AE014296 AAN11431   AE014296 AAS64922   AE014296 ACZ94576   AE014296 ACZ94577   AF417921 AAL16644   AE014296 AGB93882   AE014296 AGB93883   AE014296 AGB93884   AE014296 AGB93885   AI543653   AI945603   AW943010   AY051776 AAK93200   AY075509   BG638187   BI214618   BI370969   BP540762   BP554029   BP554973   BQ102644   BT022131   BT022285   BT023142   BT024260   CO262787   CO266706   CO272145   CO272165   CO278275   CO278718   CO287623   CO289843   CO291106   CO291240   CO291764   CO292046   CO292743   CO294338   CO294546   CO299866   CO300253   CO301886   CO305254   CO305961   CO306463   CO310000   CO311073   CO312312   CO318870   CO319497   CO323944   CO324763   CO324949   CO326459   CO327368   CO327385   CO327762   CO327857   CO328232   CO329206   CO329626   CO339868   CO343342   CO343356   CZ469293   CZ483064   CZ484742   CZ484743   CZ485558   CZ489516   CZ489517   EC057166   EC057608   EC058596   EC058709   EC060329   EC061096   EC061806   EC062705   EC063985   EC067868   EC071806   EC076180   EC077313   EC078834   EC080153   EC082017   EC195184   EC196719   EC198095   EC199090   EC199091   EC201166   EC201354   EC204457   EC212091   EC212225   EC212393   EC219425   EC221545   EC223331   EC225188   EC226194   EC227746   EC234760   EC235465   EC236501   EC237228   EC242471   EC250259   EC251832   EC251938   EC252404   EC252530   EC258104   EC258472   EC259981   EC260224   EL879098   EL884312   EL884438   EL884673   EV585284   EV595926   GH883343   GH929599   GH941260   GH941314   GH941974   GH942847   GH945483   GH947143   GH948733   GH953563   GH953792   GH957404   GH957644   GH957819   GH958232   GH960416   GH960808   GH961342   GH962108   GH962398   GH964306   GH964616   GH964717   GH969789   GH970110   GH970187   GH970949   GH971477   GH974016   GH975084   GH975554   GH976747   GH978157   GH979005   GH979307   GH982589
UniProtKB/Swiss-Prot	Q9W0T1
UniProtKB/TrEMBL	E1JHV7 E1JHV6
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism | ubiquitous   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | intense & ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | intense & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | intense & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | intense & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   organism | intense & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
NURF complex inferred from direct assay (Xiao et al., 2001)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, larval salivary gland, larval trachea, adult ovary, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    446.075 Larval Midgut    48.2 Larval Hindgut    80.5 Larval Malpighian Tubules    54.8 Larval Fat Body    61.5 Larval Salivary Gland    118 Larval Trachea    107.525 Larval Carcass    99.875 Adult Head    101.3 Adult Eye    51.925 Adult Brain    291.6 Adult Thoracic-Abdominal Ganglion    159.1 Adult Crop    75.1 Adult Midgut    57 Adult Hindgut    64.3 Adult Malpighian Tubules    45.9 Adult Fat Body    65.7 Adult Salivary Gland    74.9 Adult Heart    37.1 Adult VirginFemale Spermatheca    54.5 Adult InseminatedFemale Spermatheca    57 Adult Ovary    458.6 Adult Testis    180.5 Adult Male Accessory Gland    82.4 Adult Carcass    53.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    446.075 Larval Midgut    48.2 Larval Hindgut    80.5 Larval Malpighian Tubules    54.8 Larval Fat Body    61.5 Larval Salivary Gland    118 Larval Trachea    107.525 Larval Carcass    99.875 Adult Head    101.3 Adult Eye    51.925 Adult Brain    291.6 Adult Thoracic-Abdominal Ganglion    159.1 Adult Crop    75.1 Adult Midgut    57 Adult Hindgut    64.3 Adult Malpighian Tubules    45.9 Adult Fat Body    65.7 Adult Salivary Gland    74.9 Adult Heart    37.1 Adult VirginFemale Spermatheca    54.5 Adult InseminatedFemale Spermatheca    57 Adult Ovary    458.6 Adult Testis    180.5 Adult Male Accessory Gland    82.4 Adult Carcass    53.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    446.075 Larval Midgut    48.2 Larval Hindgut    80.5 Larval Malpighian Tubules    54.8 Larval Fat Body    61.5 Larval Salivary Gland    118 Larval Trachea    107.525 Larval Carcass    99.875 Adult Head    101.3 Adult Eye    51.925 Adult Brain    291.6 Adult Thoracic-Abdominal Ganglion    159.1 Adult Crop    75.1 Adult Midgut    57 Adult Hindgut    64.3 Adult Malpighian Tubules    45.9 Adult Fat Body    65.7 Adult Salivary Gland    74.9 Adult Heart    37.1 Adult VirginFemale Spermatheca    54.5 Adult InseminatedFemale Spermatheca    57 Adult Ovary    458.6 Adult Testis    180.5 Adult Male Accessory Gland    82.4 Adult Carcass    53.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    446.075 Larval Midgut    48.2 Larval Hindgut    80.5 Larval Malpighian Tubules    54.8 Larval Fat Body    61.5 Larval Salivary Gland    118 Larval Trachea    107.525 Larval Carcass    99.875 Adult Head    101.3 Adult Eye    51.925 Adult Brain    291.6 Adult Thoracic-Abdominal Ganglion    159.1 Adult Crop    75.1 Adult Midgut    57 Adult Hindgut    64.3 Adult Malpighian Tubules    45.9 Adult Fat Body    65.7 Adult Salivary Gland    74.9 Adult Heart    37.1 Adult VirginFemale Spermatheca    54.5 Adult InseminatedFemale Spermatheca    57 Adult Ovary    458.6 Adult Testis    180.5 Adult Male Accessory Gland    82.4 Adult Carcass    53.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    446.075 Larval Midgut    48.2 Larval Hindgut    80.5 Larval Malpighian Tubules    54.8 Larval Fat Body    61.5 Larval Salivary Gland    118 Larval Trachea    107.525 Larval Carcass    99.875 Adult Head    101.3 Adult Eye    51.925 Adult Brain    291.6 Adult Thoracic-Abdominal Ganglion    159.1 Adult Crop    75.1 Adult Midgut    57 Adult Hindgut    64.3 Adult Malpighian Tubules    45.9 Adult Fat Body    65.7 Adult Salivary Gland    74.9 Adult Heart    37.1 Adult VirginFemale Spermatheca    54.5 Adult InseminatedFemale Spermatheca    57 Adult Ovary    458.6 Adult Testis    180.5 Adult Male Accessory Gland    82.4 Adult Carcass    53.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    446.075 Larval Midgut    48.2 Larval Hindgut    80.5 Larval Malpighian Tubules    54.8 Larval Fat Body    61.5 Larval Salivary Gland    118 Larval Trachea    107.525 Larval Carcass    99.875 Adult Head    101.3 Adult Eye    51.925 Adult Brain    291.6 Adult Thoracic-Abdominal Ganglion    159.1 Adult Crop    75.1 Adult Midgut    57 Adult Hindgut    64.3 Adult Malpighian Tubules    45.9 Adult Fat Body    65.7 Adult Salivary Gland    74.9 Adult Heart    37.1 Adult VirginFemale Spermatheca    54.5 Adult InseminatedFemale Spermatheca    57 Adult Ovary    458.6 Adult Testis    180.5 Adult Male Accessory Gland    82.4 Adult Carcass    53.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    446.075 Larval Midgut    48.2 Larval Hindgut    80.5 Larval Malpighian Tubules    54.8 Larval Fat Body    61.5 Larval Salivary Gland    118 Larval Trachea    107.525 Larval Carcass    99.875 Adult Head    101.3 Adult Eye    51.925 Adult Brain    291.6 Adult Thoracic-Abdominal Ganglion    159.1 Adult Crop    75.1 Adult Midgut    57 Adult Hindgut    64.3 Adult Malpighian Tubules    45.9 Adult Fat Body    65.7 Adult Salivary Gland    74.9 Adult Heart    37.1 Adult VirginFemale Spermatheca    54.5 Adult InseminatedFemale Spermatheca    57 Adult Ovary    458.6 Adult Testis    180.5 Adult Male Accessory Gland    82.4 Adult Carcass    53.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    446.075 Larval Midgut    48.2 Larval Hindgut    80.5 Larval Malpighian Tubules    54.8 Larval Fat Body    61.5 Larval Salivary Gland    118 Larval Trachea    107.525 Larval Carcass    99.875 Adult Head    101.3 Adult Eye    51.925 Adult Brain    291.6 Adult Thoracic-Abdominal Ganglion    159.1 Adult Crop    75.1 Adult Midgut    57 Adult Hindgut    64.3 Adult Malpighian Tubules    45.9 Adult Fat Body    65.7 Adult Salivary Gland    74.9 Adult Heart    37.1 Adult VirginFemale Spermatheca    54.5 Adult InseminatedFemale Spermatheca    57 Adult Ovary    458.6 Adult Testis    180.5 Adult Male Accessory Gland    82.4 Adult Carcass    53.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    101.3   NA  Eye    51.925   NA  Brain    291.6   446.075  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    159.1   NA  Crop    75.1   48.2  Midgut    57   80.5  Hindgut    64.3   54.8  Malpighian Tubules    45.9   61.5  Fat Body    65.7   118  Salivary Gland    74.9   NA  Heart    37.1   107.525  Trachea    NA   NA  VirginFemale Spermatheca    54.5   NA  InseminatedFemale Spermatheca    57   NA  Ovary    458.6   NA  Testis    180.5   NA  Male Accessory Gland    82.4   99.875  Carcass    53.8 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    53 central nervous system, pupae P8    31 head, virgin 1-day female    15 head, virgin 4-day female    14 head, virgin 20-day female    10 head, mated 1-day female    15 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    21 head, mated 4-day male    20 head, mated 20-day male    21 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    15 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    5 digestive system, 4-day adult    10 digestive system, 20-day adult    6 fat body, larvae L3 wandering    4 fat body, white prepupae    14 fat body, pupae P8    10 carcass, larvae L3 wandering    24 carcass, 1-day adult    9 carcass, 4-day adult    12 carcass, 20-day adult    9 ovary, virgin 4-day female    119 ovary, mated 4-day female    97 testis, mated 4-day male    7 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (35) central nervous system, larvae L3  (53) central nervous system, pupae P8  (31) head, virgin 1-day female    15 head, virgin 4-day female    14 head, virgin 20-day female    10 head, mated 1-day female    15 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    21 head, mated 4-day male    20 head, mated 20-day male    21 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    15 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    5 digestive system, 4-day adult    10 digestive system, 20-day adult    6 fat body, larvae L3 wandering    4 fat body, white prepupae    14 fat body, pupae P8    10 carcass, larvae L3 wandering    24 carcass, 1-day adult    9 carcass, 4-day adult    12 carcass, 20-day adult    9 ovary, virgin 4-day female  (119) ovary, mated 4-day female  (97) testis, mated 4-day male    7 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    53 central nervous system, pupae P8    31 head, virgin 1-day female    15 head, virgin 4-day female    14 head, virgin 20-day female    10 head, mated 1-day female    15 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    21 head, mated 4-day male    20 head, mated 20-day male    21 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    15 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    5 digestive system, 4-day adult    10 digestive system, 20-day adult    6 fat body, larvae L3 wandering    4 fat body, white prepupae    14 fat body, pupae P8    10 carcass, larvae L3 wandering    24 carcass, 1-day adult    9 carcass, 4-day adult    12 carcass, 20-day adult    9 ovary, virgin 4-day female  (119) ovary, mated 4-day female    97 testis, mated 4-day male    7 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    53 central nervous system, pupae P8    31 head, virgin 1-day female    15 head, virgin 4-day female    14 head, virgin 20-day female    10 head, mated 1-day female    15 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    21 head, mated 4-day male    20 head, mated 20-day male    21 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    15 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    5 digestive system, 4-day adult    10 digestive system, 20-day adult    6 fat body, larvae L3 wandering    4 fat body, white prepupae    14 fat body, pupae P8    10 carcass, larvae L3 wandering    24 carcass, 1-day adult    9 carcass, 4-day adult    12 carcass, 20-day adult    9 ovary, virgin 4-day female    119 ovary, mated 4-day female    97 testis, mated 4-day male    7 accessory gland, mated 4-day male    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    53 central nervous system, pupae P8    31 head, virgin 1-day female    15 head, virgin 4-day female    14 head, virgin 20-day female    10 head, mated 1-day female    15 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    21 head, mated 4-day male    20 head, mated 20-day male    21 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    15 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    5 digestive system, 4-day adult    10 digestive system, 20-day adult    6 fat body, larvae L3 wandering    4 fat body, white prepupae    14 fat body, pupae P8    10 carcass, larvae L3 wandering    24 carcass, 1-day adult    9 carcass, 4-day adult    12 carcass, 20-day adult    9 ovary, virgin 4-day female    119 ovary, mated 4-day female    97 testis, mated 4-day male    7 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (35) central nervous system, larvae L3  (53) central nervous system, pupae P8  31 head, virgin 1-day female    15 head, virgin 4-day female    14 head, virgin 20-day female    10 head, mated 1-day female    15 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    21 head, mated 4-day male    20 head, mated 20-day male    21 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    15 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    5 digestive system, 4-day adult    10 digestive system, 20-day adult    6 fat body, larvae L3 wandering    4 fat body, white prepupae    14 fat body, pupae P8    10 carcass, larvae L3 wandering    24 carcass, 1-day adult    9 carcass, 4-day adult    12 carcass, 20-day adult    9 ovary, virgin 4-day female  (119) ovary, mated 4-day female  (97) testis, mated 4-day male    7 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    53 central nervous system, pupae P8    31 head, virgin 1-day female    15 head, virgin 4-day female    14 head, virgin 20-day female    10 head, mated 1-day female    15 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    21 head, mated 4-day male    20 head, mated 20-day male    21 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    15 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    5 digestive system, 4-day adult    10 digestive system, 20-day adult    6 fat body, larvae L3 wandering    4 fat body, white prepupae    14 fat body, pupae P8    10 carcass, larvae L3 wandering    24 carcass, 1-day adult    9 carcass, 4-day adult    12 carcass, 20-day adult    9 ovary, virgin 4-day female  119 ovary, mated 4-day female    97 testis, mated 4-day male    7 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    53 central nervous system, pupae P8    31 head, virgin 1-day female    15 head, virgin 4-day female    14 head, virgin 20-day female    10 head, mated 1-day female    15 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    21 head, mated 4-day male    20 head, mated 20-day male    21 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    15 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    5 digestive system, 4-day adult    10 digestive system, 20-day adult    6 fat body, larvae L3 wandering    4 fat body, white prepupae    14 fat body, pupae P8    10 carcass, larvae L3 wandering    24 carcass, 1-day adult    9 carcass, 4-day adult    12 carcass, 20-day adult    9 ovary, virgin 4-day female    119 ovary, mated 4-day female    97 testis, mated 4-day male    7 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    54 embryo 02-04hr    41 embryo 04-06hr    52 embryo 06-08hr    53 embryo 08-10hr    50 embryo 10-12hr    22 embryo 12-14hr    42 embryo 14-16hr    21 embryo 16-18hr    23 embryo 18-20hr    14 embryo 20-22hr    25 embryo 22-24hr    28 larva L1    11 larva L2    10 larva L3 12hr old    8 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    22 white prepupae new    23 white prepupae 12hr    22 white prepupae 24hr    28 pupae 2d postWPP    21 pupae 3d postWPP    13 pupae 4d postWPP    6 adult male 01day    12 adult male 05day    11 adult male 30day    9 adult female 01day    18 adult female 05day    50 adult female 30day    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (54) embryo 02-04hr  (41) embryo 04-06hr  (52) embryo 06-08hr  (53) embryo 08-10hr  (50) embryo 10-12hr    22 embryo 12-14hr  (42) embryo 14-16hr    21 embryo 16-18hr    23 embryo 18-20hr    14 embryo 20-22hr    25 embryo 22-24hr  (28) larva L1    11 larva L2    10 larva L3 12hr old    8 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    22 white prepupae new    23 white prepupae 12hr    22 white prepupae 24hr  (28) pupae 2d postWPP    21 pupae 3d postWPP    13 pupae 4d postWPP    6 adult male 01day    12 adult male 05day    11 adult male 30day    9 adult female 01day    18 adult female 05day  (50) adult female 30day  (47) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    54 embryo 02-04hr    41 embryo 04-06hr    52 embryo 06-08hr    53 embryo 08-10hr    50 embryo 10-12hr    22 embryo 12-14hr    42 embryo 14-16hr    21 embryo 16-18hr    23 embryo 18-20hr    14 embryo 20-22hr    25 embryo 22-24hr    28 larva L1    11 larva L2    10 larva L3 12hr old    8 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    22 white prepupae new    23 white prepupae 12hr    22 white prepupae 24hr    28 pupae 2d postWPP    21 pupae 3d postWPP    13 pupae 4d postWPP    6 adult male 01day    12 adult male 05day    11 adult male 30day    9 adult female 01day    18 adult female 05day    50 adult female 30day    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    54 embryo 02-04hr    41 embryo 04-06hr    52 embryo 06-08hr    53 embryo 08-10hr    50 embryo 10-12hr    22 embryo 12-14hr    42 embryo 14-16hr    21 embryo 16-18hr    23 embryo 18-20hr    14 embryo 20-22hr    25 embryo 22-24hr    28 larva L1    11 larva L2    10 larva L3 12hr old    8 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    22 white prepupae new    23 white prepupae 12hr    22 white prepupae 24hr    28 pupae 2d postWPP    21 pupae 3d postWPP    13 pupae 4d postWPP    6 adult male 01day    12 adult male 05day    11 adult male 30day    9 adult female 01day    18 adult female 05day    50 adult female 30day    47 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    54 embryo 02-04hr    41 embryo 04-06hr    52 embryo 06-08hr    53 embryo 08-10hr    50 embryo 10-12hr    22 embryo 12-14hr    42 embryo 14-16hr    21 embryo 16-18hr    23 embryo 18-20hr    14 embryo 20-22hr    25 embryo 22-24hr    28 larva L1    11 larva L2    10 larva L3 12hr old    8 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    22 white prepupae new    23 white prepupae 12hr    22 white prepupae 24hr    28 pupae 2d postWPP    21 pupae 3d postWPP    13 pupae 4d postWPP    6 adult male 01day    12 adult male 05day    11 adult male 30day    9 adult female 01day    18 adult female 05day    50 adult female 30day    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (54) embryo 02-04hr  (41) embryo 04-06hr  (52) embryo 06-08hr  (53) embryo 08-10hr  (50) embryo 10-12hr    22 embryo 12-14hr  (42) embryo 14-16hr    21 embryo 16-18hr    23 embryo 18-20hr    14 embryo 20-22hr    25 embryo 22-24hr  28 larva L1    11 larva L2    10 larva L3 12hr old    8 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    22 white prepupae new    23 white prepupae 12hr    22 white prepupae 24hr  28 pupae 2d postWPP    21 pupae 3d postWPP    13 pupae 4d postWPP    6 adult male 01day    12 adult male 05day    11 adult male 30day    9 adult female 01day    18 adult female 05day  (50) adult female 30day  (47) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    54 embryo 02-04hr    41 embryo 04-06hr    52 embryo 06-08hr    53 embryo 08-10hr    50 embryo 10-12hr    22 embryo 12-14hr    42 embryo 14-16hr    21 embryo 16-18hr    23 embryo 18-20hr    14 embryo 20-22hr    25 embryo 22-24hr    28 larva L1    11 larva L2    10 larva L3 12hr old    8 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    22 white prepupae new    23 white prepupae 12hr    22 white prepupae 24hr    28 pupae 2d postWPP    21 pupae 3d postWPP    13 pupae 4d postWPP    6 adult male 01day    12 adult male 05day    11 adult male 30day    9 adult female 01day    18 adult female 05day    50 adult female 30day    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    54 embryo 02-04hr    41 embryo 04-06hr    52 embryo 06-08hr    53 embryo 08-10hr    50 embryo 10-12hr    22 embryo 12-14hr    42 embryo 14-16hr    21 embryo 16-18hr    23 embryo 18-20hr    14 embryo 20-22hr    25 embryo 22-24hr    28 larva L1    11 larva L2    10 larva L3 12hr old    8 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    22 white prepupae new    23 white prepupae 12hr    22 white prepupae 24hr    28 pupae 2d postWPP    21 pupae 3d postWPP    13 pupae 4d postWPP    6 adult male 01day    12 adult male 05day    11 adult male 30day    9 adult female 01day    18 adult female 05day    50 adult female 30day    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    33 Schneider line 2 Sg4    10 embryonic 1182-4H    24 embryonic GM2    25 embryonic Kc167    14 embryonic S1    14 embryonic S3    35 leg disc CME L1    15 wing disc CME-W2    26 wing disc ML-DmD8    36 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    4 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1    27 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    21 ovary fGS/OSS    40 ovary OSC    40 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (33) Schneider line 2 Sg4    10 embryonic 1182-4H    24 embryonic GM2    25 embryonic Kc167    14 embryonic S1    14 embryonic S3  (35) leg disc CME L1    15 wing disc CME-W2  26 wing disc ML-DmD8  (36) wing disc ML-DmD9    10 wing disc ML-DmD16-c3  (30) wing disc ML-DmD21    4 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1  (27) CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    21 ovary fGS/OSS  (40) ovary OSC  (40) ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    33 Schneider line 2 Sg4    10 embryonic 1182-4H    24 embryonic GM2    25 embryonic Kc167    14 embryonic S1    14 embryonic S3    35 leg disc CME L1    15 wing disc CME-W2    26 wing disc ML-DmD8    36 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    4 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1    27 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    21 ovary fGS/OSS    40 ovary OSC    40 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    33 Schneider line 2 Sg4    10 embryonic 1182-4H    24 embryonic GM2    25 embryonic Kc167    14 embryonic S1    14 embryonic S3    35 leg disc CME L1    15 wing disc CME-W2    26 wing disc ML-DmD8    36 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    4 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1    27 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    21 ovary fGS/OSS    40 ovary OSC    40 ovary OSS    24 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    33 Schneider line 2 Sg4    10 embryonic 1182-4H    24 embryonic GM2    25 embryonic Kc167    14 embryonic S1    14 embryonic S3    35 leg disc CME L1    15 wing disc CME-W2    26 wing disc ML-DmD8    36 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    4 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1    27 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    21 ovary fGS/OSS    40 ovary OSC    40 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (33) Schneider line 2 Sg4    10 embryonic 1182-4H    24 embryonic GM2    25 embryonic Kc167    14 embryonic S1    14 embryonic S3  (35) leg disc CME L1    15 wing disc CME-W2  26 wing disc ML-DmD8  (36) wing disc ML-DmD9    10 wing disc ML-DmD16-c3  30 wing disc ML-DmD21    4 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1  27 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    21 ovary fGS/OSS  (40) ovary OSC  (40) ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    33 Schneider line 2 Sg4    10 embryonic 1182-4H    24 embryonic GM2    25 embryonic Kc167    14 embryonic S1    14 embryonic S3    35 leg disc CME L1    15 wing disc CME-W2    26 wing disc ML-DmD8    36 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    4 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1    27 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    21 ovary fGS/OSS    40 ovary OSC    40 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    33 Schneider line 2 Sg4    10 embryonic 1182-4H    24 embryonic GM2    25 embryonic Kc167    14 embryonic S1    14 embryonic S3    35 leg disc CME L1    15 wing disc CME-W2    26 wing disc ML-DmD8    36 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    4 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    21 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    14 CNS ML-DmBG1-c1    27 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    21 ovary fGS/OSS    40 ovary OSC    40 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    12 heat shock, 4-day adult    27 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    75 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    63 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    23 Ethanol 2.5% 3 hrs, larvae L3    57 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    58 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    36 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    12 heat shock, 4-day adult  (27) Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult  (30) Cadmium 100 mM 48 hrs, 4-day adult  (75) Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult  (63) Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    23 Ethanol 2.5% 3 hrs, larvae L3  (57) Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (58) Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult  (48) Paraquat 10 mM 48 hrs, 4-day adult  (36) Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    12 heat shock, 4-day adult    27 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    75 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    63 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    23 Ethanol 2.5% 3 hrs, larvae L3    57 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    58 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    36 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    12 heat shock, 4-day adult    27 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    75 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    63 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    23 Ethanol 2.5% 3 hrs, larvae L3    57 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    58 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    36 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    12 heat shock, 4-day adult    27 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    75 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    63 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    23 Ethanol 2.5% 3 hrs, larvae L3    57 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    58 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    36 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    12 heat shock, 4-day adult  27 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult  30 Cadmium 100 mM 48 hrs, 4-day adult  (75) Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult  (63) Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    23 Ethanol 2.5% 3 hrs, larvae L3  (57) Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (58) Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult  (48) Paraquat 10 mM 48 hrs, 4-day adult  (36) Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    12 heat shock, 4-day adult    27 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    75 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    63 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    23 Ethanol 2.5% 3 hrs, larvae L3    57 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    58 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    36 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    12 heat shock, 4-day adult    27 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    75 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    63 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    23 Ethanol 2.5% 3 hrs, larvae L3    57 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    58 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    36 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_17 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • LD30146 RE57757 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000541 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32346-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal E(bx)GD10909 lethal | germline clone | maternal effect (with Df(3L)3643) E(bx)ry122 lethal | larval stage | recessive E(bx)2 E(bx)Nurf301-2 E(bx)Nurf301-3 E(bx)Nurf301-5 E(bx)Nurf301-6 E(bx)Nurf301-7 E(bx)Nurf301-8 E(bx)Nurf301-9 lethal | recessive E(bx)1 E(bx)ry122 viable, with Scer\GAL4pnr-MD237 E(bx)GD10909 E(bx)GD15892 Other Phenotypes Allele tumorigenic E(bx)ry122 visible, with Scer\GAL4pnr-MD237 E(bx)GD10909 E(bx)GD15892 Phenotype manifest in Allele chaeta | supernumerary, with Scer\GAL4pnr-MD237 E(bx)GD10909 dendrite E(bx)ry122 embryonic/larval brain | larval stage E(bx)GD10909 embryonic/larval hemocyte | supernumerary E(bx)ry122 hemocyte | supernumerary E(bx)Nurf301-2 E(bx)Nurf301-4 melanotic mass E(bx)Nurf301-2 melanotic mass (with E(bx)Nurf301-2) E(bx)Nurf301-3 E(bx)Nurf301-4 E(bx)ry122 melanotic mass (with E(bx)Nurf301-3) E(bx)Nurf301-2 E(bx)ry122 melanotic mass (with E(bx)Nurf301-4) E(bx)Nurf301-2 melanotic mass (with E(bx)ry122) E(bx)Nurf301-2 E(bx)Nurf301-3 mesothoracic tergum, with Scer\GAL4pnr-MD237 E(bx)GD10909 E(bx)GD15892 polytene chromosome E(bx)Nurf301-2 polytene chromosome (with Df(3L)3643) E(bx)Nurf301-10 E(bx)Nurf301-12 E(bx)Nurf301-2 E(bx)Nurf301-3 E(bx)Nurf301-4 E(bx)Nurf301-5 polytene chromosome (with E(bx)Nurf301-2) E(bx)Nurf301-3 polytene chromosome (with E(bx)Nurf301-3) E(bx)Nurf301-2
Classical Alleles ( 25 )
For All Classical Alleles Show
Allele of E(bx) Class Mutagen Stocks Known lesion E(bx)c04498 piggyBac transposase 1 -- E(bx)CB-5678-3 1 -- E(bx)d02664 1 -- E(bx)f00603 piggyBac transposase 1 -- E(bx)f03002 piggyBac transposase 1 -- E(bx)G4403 P-element activity 1 -- E(bx)MI02553 1 -- E(bx)NP5364 1 -- E(bx)Nurf301-3 ethyl methanesulfonate 1 Yes E(bx)Nurf301-4 ethyl methanesulfonate 1 Yes E(bx)ry122 P-element activity 1 Yes E(bx)1 X ray 0 -- E(bx)2 spontaneous 0 -- E(bx)f02236 piggyBac transposase 0 -- E(bx)f06789 piggyBac transposase 0 -- E(bx)Nurf301-10 ethyl methanesulfonate 0 Yes E(bx)Nurf301-11 ethyl methanesulfonate 0 Yes E(bx)Nurf301-12 ethyl methanesulfonate 0 Yes E(bx)Nurf301-13 ethyl methanesulfonate 0 Yes E(bx)Nurf301-2 ethyl methanesulfonate 0 Yes E(bx)Nurf301-5 ethyl methanesulfonate 0 Yes E(bx)Nurf301-6 ethyl methanesulfonate 0 Yes E(bx)Nurf301-7 ethyl methanesulfonate 0 Yes E(bx)Nurf301-8 ethyl methanesulfonate 0 Yes E(bx)Nurf301-9 ethyl methanesulfonate 0 Yes
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of E(bx) Class Mutagen Stocks Known lesion E(bx)GD10909 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes E(bx)GD15892 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes E(bx)GL00265 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes E(bx)HMS00065 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes E(bx)JF01709 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)3643 (Badenhorst et al., 2002, Badenhorst, 2003.3.11) Df(3L)Exel6084 (Ryder, 2004.4.26) Df(3L)rH321 (Badenhorst et al., 2002, Badenhorst, 2003.3.11, Badenhorst, 2003.3.11) Df(3L)XZB970 (Badenhorst et al., 2002, Badenhorst, 2003.3.11)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10909} NA P{GD15892} NA P{TRiP.GL00265} NA P{TRiP.HMS00065} NA P{TRiP.JF01709} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}E(bx)G4403 NA P{EP}EP637 NA P{EP}EP3495 NA P{EP}EP3643 NA P{EP}mthl14EP3410 NA P{GSV1}EP-945 NA P{GSV1}GSd066GSd066 NA P{GSV1}GSd451GSd451 NA P{GSV1}GSd492GSd492 NA P{XP}d02664 NA miscellaneous insertions Mi{MIC}E(bx)MI02553 NA P{RS3}E(bx)CB-5678-3 NA P{ry11}E(bx)ry122 NA P{SUPor-P}KG03686 NA P{Switch2}GSG4102 NA PBac{PB}E(bx)c04498 NA PBac{WH}E(bx)f00603 NA PBac{WH}E(bx)f02236 NA PBac{WH}E(bx)f03002 NA PBac{WH}E(bx)f06789 NA insertion of enhancer trap binary system P{GawB}E(bx)NP5364 No insertion of enhancer trap P{PZ}02281b No
Gene Ontology: Function, Process & Cellular Component ( 17 unique terms )
Terms Based on Experimental Evidence ( 14 terms )
Molecular Function
CV term Evidence References
ligand-dependent nuclear receptor binding inferred from physical interaction with EcR (Badenhorst et al., 2005)
Biological Process
CV term Evidence References
chromatin organization inferred from mutant phenotype (Bai et al., 2007) chromatin remodeling inferred from mutant phenotype (Badenhorst et al., 2002) dendrite morphogenesis inferred from mutant phenotype (Parrish et al., 2006) ecdysone receptor-mediated signaling pathway inferred from genetic interaction with EcR (Badenhorst et al., 2005) hemopoiesis inferred from mutant phenotype (Badenhorst et al., 2002) lateral inhibition inferred from mutant phenotype (Mummery-Widmer et al., 2009) mitotic G2 DNA damage checkpoint inferred from genetic interaction with nbs (Kondo and Perrimon, 2011) nucleosome mobilization inferred from direct assay (Hamiche et al., 1999, Xiao et al., 2001) positive regulation of Notch signaling pathway inferred from genetic interaction with N (Kugler and Nagel, 2010) inferred from mutant phenotype (Kugler and Nagel, 2010) pupariation inferred from mutant phenotype (Badenhorst et al., 2005) regulation of transcription from RNA polymerase II promoter inferred from mutant phenotype (Li et al., 2010) transcription, DNA-dependent inferred from direct assay (Mizuguchi et al., 2001) inferred from mutant phenotype (Badenhorst et al., 2002)
Cellular Component
CV term Evidence References
NURF complex inferred from direct assay (Xiao et al., 2001)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from electronic annotation with InterPro:IPR017956 (FlyBase Curators et al., 2004-) zinc ion binding inferred from electronic annotation with InterPro:IPR001965 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
nucleosome assembly non-traceable author statement (Xiao, 2001.9.7) nucleosome mobilization traceable author statement (Lusser and Kadonaga, 2003)
Cellular Component
CV term Evidence References
NURF complex non-traceable author statement (Xiao, 2001.9.7) traceable author statement (Badenhorst et al., 2002)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
E(bx) - CG10496 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) E(bx) - CG11455 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions E(bx) allele Gene References E(bx)Nurf301-2 hop (Badenhorst et al., 2002) os (Badenhorst et al., 2002) Ubx (Badenhorst et al., 2002) unspecified EcR (Badenhorst et al., 2005) roX1 (Bai et al., 2007) roX2 (Bai et al., 2007) Ubx (Bhosekar and Babu, 1987, Lindsley and Zimm, 1992)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 69297 DroID - A comprehensive database of gene and protein interactions. • FBgn0000541 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 44811
Orthologs
OrthoDB Orthologs (53) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6PG6K5 Dipteran inclusive ortholog search EOG66WZHB Insect inclusive ortholog search EOG60RZ9F Arthropod inclusive ortholog search EOG61JWSX Metazoa inclusive ortholog search EOG683RX4
Orthologs in Drosophila Species (EOG6PG6K5)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\E(bx)     Drosophila simulans   Dsim\GD13529 Y   Drosophila sechellia   Dsec\GM14291     Drosophila sechellia   Dsec\GM14290     Drosophila erecta   Dere\GG14675 Y   Drosophila yakuba   Dyak\GE21036 Y   Drosophila ananassae   Dana\GF24755 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA16840 Y   Drosophila persimilis   Dper\GL16133 Y   Drosophila willistoni   Dwil\GK17657 Y   Drosophila virilis   Dvir\GJ16046     Drosophila virilis   Dvir\GJ16047     Drosophila mojavensis   Dmoj\GI12529 Y   Drosophila grimshawi   Dgri\GH15750 Y
Orthologs in non-Drosophila Dipterans (EOG66WZHB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL012607   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL015015   Anopheles gambiae Malaria mosquito  Agam\AGAP006133   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ011284   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ011285
Orthologs in non-Dipteran Insects (EOG60RZ9F)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB49172   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG004919   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH13583   Atta cephalotes Leafcutter ant  Acep\ACEP11718   Camponotus floridanus Florida carpenter ant  Cflo\CFLO13740   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL19148   Linepithema humile Argentine ant  Lhum\LH23880   Pogonomyrmex barbatus Red harvester ant  Pbar\PB26099   Solenopsis invicta Red fire ant  Sinv\SINV10466   Solenopsis invicta Red fire ant  Sinv\SINV10483   Acyrthosiphon pisum Pea aphid  Apim\ACYPI006222   Bombyx mori Silkmoth  Bmor\BGIBMGA006818   Pediculus humanus Human body louse  Phum\PHUM320320   Tribolium castaneum Red flour beetle  Tcas\TC012345
Orthologs in non-Insect Arthropods (EOG61JWSX)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_264419   Daphnia pulex Water flea  Dpux\Prkab2   Daphnia pulex Water flea  Dpux\JGI_V11_37793   Daphnia pulex Water flea  Dpux\JGI_V11_247464   Daphnia pulex Water flea  Dpux\rbcL   Daphnia pulex Water flea  Dpux\otp   Daphnia pulex Water flea  Dpux\JGI_V11_1432   Daphnia pulex Water flea  Dpux\MAP4K4   Daphnia pulex Water flea  Dpux\Aar2   Daphnia pulex Water flea  Dpux\petB   Ixodes scapularis Deer tick  Isca\ISCW023060   Ixodes scapularis Deer tick  Isca\ISCW012439
Orthologs in non-Arthropod Metazoa (EOG683RX4)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\F26H11.2   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_025832gn   Danio rerio Zebrafish  Drer\BX085195.1   Xenopus tropicalis Western clawed frog  Xtro\bptf   Gallus gallus Domestic chicken  Ggal\BPTF   Mus musculus House mouse  Mmus\Bptf   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000015626   Homo sapiens Human  Hsap\ENSG00000171634
Human Orthologs (1)
Gene OMIM HGNC Hsap\ENSG00000171634
AAA Orthologs (9) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD13529 Drosophila erecta Dere\GG14675 Drosophila yakuba Dyak\GE21036 Drosophila ananassae Dana\GF24755 Drosophila pseudoobscura pseudoobscura Dpse\GA16840 Drosophila persimilis Dper\GL16133 Drosophila willistoni Dwil\GK17657 Drosophila mojavensis Dmoj\GI12529 Drosophila grimshawi Dgri\GH15750
Stocks & Reagents
Stocks Listed in FlyBase ( 16 )
Bloomington	27471 w*; P{EP}E(bx)G4403 31193 y1 v1; P{TRiP.JF01709}attP2 9904 w1118; E(bx)Nurf301-4/TM6B, Tb1 35353 y1 sc* v1; P{TRiP.GL00265}attP2 33658 y1 sc* v1; P{TRiP.HMS00065}attP2
Harvard	- P{XP}d02664 - PBac{PB}E(bx)c04498 - PBac{WH}E(bx)f00603 - PBac{WH}E(bx)f03002
Kyoto	123751 w1118; P{RS3}E(bx)CB-5678-3
VDRC	v46645 w1118 P{GD15892}v46645 v24740 w1118; P{GD10909}v24740
	More stocks available...
Genomic Clones ( 2 )
	BACR11J11 BACR13F06 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 141 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP04685 IP08836 IP08936 IP15833 LD30146 RE57757
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE20584 SD10523
Other clones	8036 8068 11940 17282 18178 36541 36565 39687 57411 58982 60125 65398 67089 70435 71027 72389 74907 76730 97467 100578 101395 107608 125602 144969 174102 190246 190759 214217.26 214221.30 214221.31 214315.28 214385.25 214409.31 214437.33 214513.28 277642 bs14f11 CK01.9C.F8 CK01.14B.E7 CK01.34C.D11 CK01.40A.F3 CK01.83A.G3 CK01.88A.E11 CK01.98C.D6 CK01.99D.G8 CK01.102C.E6 Dmel_dig_012847_0583_2182 Dmel_neb_010116_0703_0450 EK066326 EK072218 EK076136 EK076860 EK086351 EK106014 EK106045 EK129622 EK131324 EK147311 EK176760 EK177748 EK182513 EK192019 EK194804 EK195225 EK198127 EK199053 EK200854 EK206612 EK207155 EK220227 EK223261 EK228416 EK247755 EK249908 EK251117 EK274558 EK278368 EK292248 EK293256 EP03947 EP06847 EP06901 EP08003 EP08147 EP08808 EP09537 EP16620 EP16636 FGA007G03 FGM227F03 FGM232G03 IP09036 IP15633 IP15733 IP15933 LD19161 RP003445091 RP003465340 RP004039697 RP004049553 RP004053984 RP004058371 RP004124572 RP004127400 RP004194245 RP004212739 RP004219322 RP004229975 RP004251761 RP004255548 RP004256920 RP004261032 RP004263412 RP004267405 RP004269581 RP004311738 RP004340169 RP004350617 RP004371978 RP004411671 RP004420037 RP004430706 RP004431971 RP004436665 RP004501295 RP004501661 RP004505301 RP004525555 RP004528268 RP004535873 RP004541894 RP004545387 UUGC0018
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0000541 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 44811
Antibody Information
Other Information
Discoverer
	E. B. Lewis.
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: E(bx) Nurf301 (FlyBase, 1996-)
Source for database merge of	Source for merge of: E(bx) CG10894 (Badenhorst and Wu, 2001, FlyBase Genome Annotators, 2002-2003) Source for merge of: E(bx) l(3)ry122 (Badenhorst et al., 2002) Source for merge of: Nurf301 CG17135 CG7022 (FlyBase, 1996-)
Additional comments	Annotations CG10894, CG17135 and CG7022 merged as CG32346 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) "p230", "p250" and "p300" do not correspond to "E(bx)". (Hochheimer et al., 2002) Source for merge of Nurf301 CG17135 CG7022 was sequence comparison (date:011022). (Badenhorst and Wu, 2001, FlyBase, 1996-)
Other Comments
	dsRNA has been made from templates generated with primers directed against this gene. RNAi of E(bx) results in increased arborization of ddaD and ddaE neurons, defects in dendrite morphogenesis and reproducible defects in da dendrite development. (Parrish et al., 2006) E(bx) protein binds EcR protein in an ecdysone-dependent manner. (Badenhorst et al., 2005) E(bx) is required for induction of the heat shock genes and has additional functions during larval blood development. (Badenhorst et al., 2002) The NURF complex (composed of Caf1, E(bx), Iswi and Nurf-38 proteins) can remodel chromatin and stimulate transcription in vitro irrespective of the acetylation status of histones. (Mizuguchi et al., 2001) E(bx) is a component of the NURF complex that is both necessary and sufficient for accurate and efficient nucleosome sliding. It interacts with sequence-specific transcription factors, providing a basis for targeted recruitment of the NURF complex to specific genes. (Xiao et al., 2001) NURF, a protein complex of four subunits Caf1, Nurf-38, E(bx) and Iswi, is able to facilitate transcription mediated by a Scer\GAL4 derivative carrying a constitutively activating region from Hsf. Once nucleosome remodelling by the DNA binding factor is accomplished a high level of NURF activity is not continuously required for recruitment of the general transcription machinery and transcription initiation. NURF is able to assist gene activation in a chromatin context: remodel nucleosomes for gene activation in chromatin. (Mizuguchi et al., 1997) Nucleosome remodelling factor is composed of at least four polypeptides that act in concert with the Trl transcription factor at the Hsp70 promoter. Nucleosome remodelling factor acts directly on the nucleosome to perturb its structure. (Tsukiyama and Wu, 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_167817 NM_167819 NM_206224 NM_001169833 NM_001169834 NM_001274258 NM_001274259 NM_001274260 NM_001274261 RefSeq (Proteins) • NP_728505 NP_728507 NP_995946 NP_001163304 NP_001163305 NP_001261187 NP_001261188 NP_001261189 NP_001261190 DDBJ / EMBL / GenBank • AA950197 AAF47361 AAK93200 AAL16644 AAN11431 AAS64922 ACZ94576 ACZ94577 AF417921 AGB93882 AGB93883 AGB93884 AGB93885 AI945603 AW943010 AY051776 AY075509 BI370969 CZ469293 CZ483064 CZ484742 CZ484743 CZ485558 CZ489516 CZ489517 Entrez Gene - A searchable database of RefSeq genes. • 44811 UniProtKB/Swiss-Prot • Q9W0T1 UniProtKB/TrEMBL • E1JHV6 E1JHV7
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • AT hook, DNA-binding motif (IPR017956) Bromodomain, conserved site (IPR018359) DDT domain, subgroup (IPR018500) DDT domain superfamily (IPR018501) Bromodomain (IPR001487) Zinc finger, PHD-type (IPR001965) DDT domain (IPR004022) Zinc finger, FYVE/PHD-type (IPR011011) Zinc finger, PHD-type, conserved site (IPR019786) Zinc finger, PHD-finger (IPR019787) Zinc finger, RING/FYVE/PHD-type (IPR013083) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD30146 RE57757
Linkouts
	BioGRID - A database of protein and genetic interactions • 69297 DroID - A comprehensive database of gene and protein interactions. • FBgn0000541 DRSC - Results from RNAi screens. • FBgn0000541 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000541 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32346-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0000541 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 44811 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /polycomb/nurf3011.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 44811 modMine - Data generated by the modENCODE project. • FBgn0000541
Synonyms & Secondary IDs ( 30 )
Reported As
Symbol Synonym	CG7022   CG10894 (Oliver, 2001.8.16, ) CG17135 (Doerks et al., 2001, Sanjuan and Marin, 2001, Molnar et al., 2006) CG32346 (Klebes et al., 2005, Bienz, 2006, Ni et al., 2008, Ni et al., 2010.12.1, Ni et al., 2011.7.19, Lowery et al., 2010) CG32478   dNURF (Fry and Peterson, 2001, Boyer et al., 2000, Peterson, 2000) dNURF301 (Kessler et al., 2009) E(Bx) (Lewis et al., 2004) E(bx) (Wysocka et al., 2006, Cavalli, 2006, Hoskins et al., 2005, Parrish et al., 2006, Nicholson et al., 2008, Englund et al., 2006, Arancio et al., 2010, Molnar et al., 2006, Minidorff et al., 2007, Xing et al., 2007, Kallappagoudar et al., 2010, Blard et al., 2007, Zhou et al., 2008, Burgio et al., 2008, Wasbrough et al., 2010, Lowery et al., 2010, Popkova et al., 2012, Korenjak et al., 2012, Cole et al., 2012.7.6) E(BX) (Kondo and Perrimon, 2011) Ebx (Li et al., 2010) En-bx   l(3)122 (Bloomington Drosophila Stock Center, 2000.1.31, ) l(3)ry122 (Berg and Spradling, 1991, ) NURF (Varga-Weisz, 2006, Baumann et al., 2003, Shen et al., 2003, Wu, 2001, Kang et al., 2002, Ridgway and Almouzni, 2001, Reeves, 2001, Mizuguchi et al., 2001, Jones and Kadonaga, 2000, Farkas et al., 2000, Glass and Rosenfeld, 2000, Tyler and Kadonaga, 1999, Guschin and Wolffe, 1999, Di Croce et al., 1999, Kornberg and Lorch, 1999, Wu et al., 1998, Reinberg et al., 1998, Jacobson and Pillus, 1999, Travers, 1999, John and Workman, 1998, Pollard and Peterson, 1998, Gregory and Horz, 1998, Armstrong and Emerson, 1998, Luger and Richmond, 1998, Mizuguchi et al., 1997, Kadonaga, 1998, Gregory and Horz, 1998, Ito et al., 1997, Tsukiyama and Wu, 1997, Peterson, 1996, Kingston et al., 1996, Wysocka et al., 2006) NURF215 (Xiao et al., 2001) NURF-215 (Hamiche et al., 1999) Nurf-215 (Bjorklund et al., 1999, Tsukiyama and Wu, 1995, ) Nurf301 (Badenhorst et al., 2005, Jacquier et al., 2007, Bai et al., 2007, Kugler et al., 2011, Petesch and Lis, 2008, Kwon et al., 2008, Carré et al., 2008, Nakayama et al., 2012, Kugler and Nagel, 2010, Li et al., 2010) NURF301 (Eberharter and Becker, 2004, Lewis et al., 2004, Schwanbeck et al., 2004, Lusser and Kadonaga, 2003, Xiao et al., 2001, Emerson, 2002, Wysocka et al., 2006, Bienz, 2006, Cherry and Matunis, 2008, Kharchenko et al., 2011, Larschan et al., 2012, Kwon et al., 2009, Shi et al., 2006, Ferreira et al., 2007, Wang et al., 2013, Sakoparnig et al., 2012, Stephens et al., 2006, Demakov et al., 2011, Li et al., 2010) nurf301 (Lehmann, 2004, Evans et al., 2003, Badenhorst, 2003.3.11, Badenhorst et al., 2002, Song et al., 2009, Ables and Drummond-Barbosa, 2010) NURF-301 (Hochheimer et al., 2002, Cavalli, 2006) NuRF301 (Kaeser and Emerson, 2006) Nurf 301 (Kugler and Nagel, 2010) p215 (Neely and Workman, 2002) p301 (Langst and Becker, 2001, Badenhorst and Wu, 2001, Badenhorst et al., 2000)
Name Synonym	Enhancer of bithorax (Xing et al., 2007, Blard et al., 2007) Nucleosome remodeling factor (Kingston et al., 1996) nucleosome remodeling factor (Kwon et al., 2008) Nucleosome remodeling factor - 215kD (Tsukiyama and Wu, 1995)
Secondary FlyBase IDs
	FBgn0013553 FBgn0016686 FBgn0035116 FBgn0035117 FBgn0035118 FBgn0061199
References ( 137 )
Generate a list of	All references relating to this gene ( 137 )
List References by type	Research paper  ( 63 ) Supplementary material  ( 8 ) Review  ( 34 ) Personal communication to FlyBase  ( 15 ) Abstract  ( 5 ) FlyBase analysis  ( 4 ) Conference report  ( 2 ) DNA/RNA sequence record  ( 1 ) Website  ( 1 ) Book  ( 1 ) Curated genome annotation  ( 3 )
Recent research papers ( 12 )
	Wang et al., 2013, Nat. Struct. Mol. Biol. 20(2): 202--209 Chromatin proteins captured by ChIP-mass spectrometry are linked to dosage compensation in Drosophila. [FBrf0220693] Acharya et al., 2012, G3 (Bethesda) 2(11): 1459--1472 Evidence for autoregulation and cell signaling pathway regulation from genome-wide binding of the Drosophila retinoblastoma protein. [FBrf0220009] Korenjak et al., 2012, Mol. Cell. Biol. 32(21): 4375--4387 RBF Binding to both Canonical E2F Targets and Noncanonical Targets Depends on Functional dE2F/dDP Complexes. [FBrf0219643] Larschan et al., 2012, PLoS Genet. 8(7): e1002830 Identification of Chromatin-Associated Regulators of MSL Complex Targeting in Drosophila Dosage Compensation. [FBrf0219020] Nakayama et al., 2012, Development 139(24): 4582--4590 The PBAP remodeling complex is required for histone H3.3 replacement at chromatin boundaries and for boundary functions. [FBrf0219981] Popkova et al., 2012, PLoS Genet. 8(12): e1003159 Polycomb controls gliogenesis by regulating the transient expression of the gcm/glide fate determinant. [FBrf0220515] Sakoparnig et al., 2012, J. Comput. Biol. 19(2): 126--138 Binding profiles of chromatin-modifying proteins are predictive for transcriptional activity and promoter-proximal pausing. [FBrf0217402] Demakov et al., 2011, BMC Genomics 12: 566 Protein composition of interband regions in polytene and cell line chromosomes of Drosophila melanogaster. [FBrf0216974] Kharchenko et al., 2011, Nature 471(7339): 480--485 Comprehensive analysis of the chromatin landscape in Drosophila melanogaster. [FBrf0213294] Kirilly et al., 2011, Neuron 72(1): 86--100 Intrinsic epigenetic factors cooperate with the steroid hormone ecdysone to govern dendrite pruning in Drosophila. [FBrf0216319] Kondo and Perrimon, 2011, Sci. Signal. 4(154): rs1 A Genome-Wide RNAi Screen Identifies Core Components of the G2-M DNA Damage Checkpoint. [FBrf0212700] Kugler et al., 2011, Genetics 188(1): 127--139 The Putzig-NURF Nucleosome Remodeling Complex Is Required for Ecdysone Receptor Signaling and Innate Immunity in Drosophila melanogaster. [FBrf0213648] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...