The temporal pattern of EcR RNA expression was carefully studied and related to times of ecdysone pulses. Peaks of the 5kb EcR-A RNA occur in mid-embryogenesis and early and late in pupation.

(Talbot et al., 1993)

The temporal pattern of EcR RNA expression was carefully studied and related to times of ecdysone pulses. The strongest peak of EcR-B1 RNA expression occurs in late third instar larvae. Smaller peaks are observed during late embryonic, late first and second instar larval, and mid pupal stages.

(Talbot et al., 1993)

The 6 kb EcR transcript is detected in all stages except for 0-3 hr embryos. Expression is most abundant in embryos and in late third instar larvae and early pupae, with highest levels seen in wandering third instar larvae.

(Koelle et al., 1991)

EcR staining is widespread in the adult brain.

(Ishimoto et al., 2009)

EcR protein is located in the nucleus in embryonic, late third instar larval and pupal tissues. EcR protein localizes to the nuclei of the cell lines in which it is overexpressed, even in the absence of ecdysone.

(Jang et al., 2009, Koelle et al., 1991)

EcR is expressed in approximately 20 antennal lobe projection neurons, 18 of which are located in a anterodorsal cluster.

(Marin et al., 2005)

EcR-A and EcR-B1 protein staining is observed in follicle cells and nurse cells throughout egg chamber development.

(Carney and Bender, 2000)

Most neurons (type I neurons) express the "EcR-A" isoform at low levels throughout adult development. Approximately 300 neurons (type II neurons) in the pupal ventral CNS express "EcR-A", at about 10-fold higher levels than found in type I neurons. Expression is detected in about 29 cells at 12 hr APF, increases to about 300 neurons by 42 hr APF, and continues to be detected through the rest of pupal stages. The majority of the neurons are located in the abdominal and third thoracic neuromeres, with about 24 in in neuromeres T1 and T2. The neurons which express "EcR-A" at high levels undergo rapid degeneration in newly eclosed adults. The death of the type II neurons can be delayed by 20-hydroxyecdysone treatment.

(Robinow et al., 1993)

At the onset of metamorphosis, the EcR-A and EcR-B1 protein isoforms are expressed in most tissues and are found in approximately complimentary patterns. EcR-A protein is expressed predominantly in large imaginal discs and rings that achieve their size by cell division during metamorphosis. EcR-B1 protein is found mainly in tissues that are strictly larval and in small cell clusters that respond to ecdysone by extensive cell multiplication, such as histoblast nests and midgut imaginal islands. A temporal study of EcR-B1 protein expression through development shows that it is present from mid to late embryogenesis, is at low levels in larval stages and is higher again during pupal stages.

(Talbot et al., 1993)

At the onset of metamorphosis, the EcR-A and EcR-B1 protein isoforms are expressed in most tissues and are found in approximately complimentary patterns. EcR-A protein is expressed predominantly in large imaginal discs and rings that achieve their size by cell division during metamorphosis. EcR-B1 protein is found mainly in tissues that are strictly larval and in small cell clusters that respond to ecdysone by extensive cell multiplication, such as histoblast nests and midgut imaginal islands. A temporal study of EcR-A protein expression through development shows that it is present throughout embryonic and pupal stages and is present at low levels in first instar larvae.

(Talbot et al., 1993)

A 105 kD EcR protein is detected in embryos, in late third instar larvae and in early pupae. Low levels of protein are also detected in first instar larvae and adults, but no protein is detected in second and early third instar larvae. EcR protein is distributed throughout embryonic, late third instar larval, and prepupal tissues and localizes to the nucleus.

(Koelle et al., 1991)