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General Information
Symbol
Dmel\EcR
Species
D. melanogaster
Name
Ecdysone receptor
Annotation Symbol
CG1765
Feature Type
FlyBase ID
FBgn0000546
Gene Model Status
Stock Availability
Gene Snapshot
Ecdysone receptor (EcR) encodes a protein that interacts with the product of usp to form the nuclear ecdysone receptor heterodimer, which modulates, in conjunction with co-activators and co-repressors, the activities of hundreds of genes in a tissue- and stage-specific way. EcR is widely expressed in embryonic and larval tissues and in some adult tissues where its activities (modulated by the hormone ecdysone) trigger both molting and metamorphosis. [Date last reviewed: 2019-03-07]
Also Known As
EcR-B1, EcRB1, DmEcR, EcR-A, ecdysteroid receptor
Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:6,087,873..6,169,087 [-]
Recombination map
2-55
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
Belongs to the nuclear hormone receptor family. NR1 subfamily. (P34021)
Summaries
Gene Group (FlyBase)
NUCLEAR RECEPTOR (LIGAND-DEPENDENT) TRANSCRIPTION FACTORS -
Nuclear receptors (NR) are C4 zinc finger ligand-dependent DNA-binding transcription factors. Members of the NR superfamily are defined by the presence of a highly conserved DNA-binding domain and a less conserved C-terminal ligand-binding domain. (Adapted from FBrf0184203).
Protein Function (UniProtKB)
Receptor for ecdysone (PubMed:1913820, PubMed:30293839). Binds to ecdysone response elements (ECRES) following ecdysone-binding, and recruitment of a complex containing the histone methyltransferase trr, leads to activate transcription of target genes (PubMed:1913820, PubMed:30293839).
(UniProt, P34021)
Summary (Interactive Fly)
transcription factor - nuclear receptor - zinc finger - regulates molting cycles and oogenesis
Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
3

Please see the GBrowse view of Dmel\EcR or the JBrowse view of Dmel\EcR for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0086008
5108
849
FBtr0086011
4092
878
FBtr0086012
4984
669
FBtr0086009
3740
849
FBtr0086010
5233
849
FBtr0302439
8415
878
Additional Transcript Data and Comments
Reported size (kB)
6, 5, 4 (northern blot)
6.0 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0085349
91.2
849
7.71
FBpp0085352
93.9
878
6.88
FBpp0085353
73.3
669
6.76
FBpp0085350
91.2
849
7.71
FBpp0085351
91.2
849
7.71
FBpp0291631
93.9
878
6.88
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

849 aa isoforms: EcR-PA, EcR-PD, EcR-PE
878 aa isoforms: EcR-PB, EcR-PG
Additional Polypeptide Data and Comments
Reported size (kDa)
878, 849, 669 (aa); 105, 80 (kD)
878 (aa); 105 (kD observed)
Comments
Three EcR isoforms exist that differ in their N-terminal sequences. They have 652 C-terminal amino acids in common that contain the DNA- and hormone-binding domains. All were shown to function as ecdysone receptors in S2 cells. Monoclonal antibodies have been produced that specifically recognize two of the three EcR isoforms (all but EcR-B2). Monoclonal antibodies have also been produced against the region common to all of the EcR proteins.
Three EcR isoforms exist that differ in their N-terminal sequences. They have 652 C-terminal amino acids in common that contain the DNA- and hormone-binding domains. All were shown to function as ecdysone receptors in S2 cells. Monoclonal antibodies have been produced that specifically recognize two of the three EcR isoforms (all but EcR-B2 protein). Monoclonal antibodies have also been produced against the region common to all of the EcR proteins.
alternatively spliced exon
Monoclonal antibody was generated against amino acids 67-878 of this protein. Polyclonal antibody was generated against amino acids 335-447 of this protein. EcR protein expressed in Drosophila Schneider 2 cells is able to bind the ecdysone analog iodoponasterone A, and binding activity is lost by treatment with EcR antibody. Embryonic extracts also show ecdysone analog binding which is removable by antibody treatment. EcR protein is able to specifically bind ecdysone response element (EcRE) DNA.
External Data
Subunit Structure (UniProtKB)
Heterodimer of USP and ECR (PubMed:8247157). Only the heterodimer is capable of high-affinity binding to ecdysone (PubMed:8247157). Interacts with trr in an ecdysone-dependent manner (PubMed:14603321). Upon ecdysone stimulation, interacts with Nup98 (PubMed:28366641).
(UniProt, P34021)
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\EcR using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (82 terms)
Molecular Function (17 terms)
Terms Based on Experimental Evidence (11 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from physical interaction with FLYBASE:usp; FB:FBgn0003964
inferred from physical interaction with FLYBASE:Utx; FB:FBgn0260749
inferred from physical interaction with FLYBASE:rig; FB:FBgn0250850
inferred from physical interaction with UniProtKB:Q9VCH5
(assigned by UniProt )
inferred from physical interaction with FLYBASE:usp; FB:FBgn0003964
inferred from direct assay
Terms Based on Predictions or Assertions (11 terms)
CV Term
Evidence
References
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000637751
(assigned by GO_Central )
inferred from sequence or structural similarity with MGI:MGI:1352464
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000637751
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000637751
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000637751
(assigned by GO_Central )
non-traceable author statement
inferred from electronic annotation with InterPro:IPR001723
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN000637751
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000637751
(assigned by GO_Central )
inferred from electronic annotation with InterPro:IPR001628, InterPro:IPR013088
(assigned by InterPro )
Biological Process (57 terms)
Terms Based on Experimental Evidence (47 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:if; FB:FBgn0001250
inferred from genetic interaction with FLYBASE:mys; FB:FBgn0004657
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:babo; FB:FBgn0011300
inferred from genetic interaction with FLYBASE:Smox; FB:FBgn0025800
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:InR; FB:FBgn0283499
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (14 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000637751
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000637752
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000637752
(assigned by GO_Central )
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000637752
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000637752
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000637751
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000637752
(assigned by GO_Central )
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000637751
(assigned by GO_Central )
Cellular Component (8 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000637751
(assigned by GO_Central )
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000637751
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference

Comment: reference states 0-3 hr AEL

in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference

Comment: reference states >=3 hr AEL

Additional Descriptive Data
The temporal pattern of EcR RNA expression was carefully studied and related to times of ecdysone pulses. Peaks of the 5kb EcR-A RNA occur in mid-embryogenesis and early and late in pupation.
The temporal pattern of EcR RNA expression was carefully studied and related to times of ecdysone pulses. The strongest peak of EcR-B1 RNA expression occurs in late third instar larvae. Smaller peaks are observed during late embryonic, late first and second instar larval, and mid pupal stages.
The 6 kb EcR transcript is detected in all stages except for 0-3 hr embryos. Expression is most abundant in embryos and in late third instar larvae and early pupae, with highest levels seen in wandering third instar larvae.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
neuron | subset

Comment: reference states >=12 hr APF

ventral nervous system | restricted

Comment: reference states >=12 hr APF

western blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
EcR staining is widespread in the adult brain.
EcR protein is located in the nucleus in embryonic, late third instar larval and pupal tissues. EcR protein localizes to the nuclei of the cell lines in which it is overexpressed, even in the absence of ecdysone.
EcR is expressed in approximately 20 antennal lobe projection neurons, 18 of which are located in a anterodorsal cluster.
EcR-A and EcR-B1 protein staining is observed in follicle cells and nurse cells throughout egg chamber development.
Most neurons (type I neurons) express the "EcR-A" isoform at low levels throughout adult development. Approximately 300 neurons (type II neurons) in the pupal ventral CNS express "EcR-A", at about 10-fold higher levels than found in type I neurons. Expression is detected in about 29 cells at 12 hr APF, increases to about 300 neurons by 42 hr APF, and continues to be detected through the rest of pupal stages. The majority of the neurons are located in the abdominal and third thoracic neuromeres, with about 24 in in neuromeres T1 and T2. The neurons which express "EcR-A" at high levels undergo rapid degeneration in newly eclosed adults. The death of the type II neurons can be delayed by 20-hydroxyecdysone treatment.
At the onset of metamorphosis, the EcR-A and EcR-B1 protein isoforms are expressed in most tissues and are found in approximately complimentary patterns. EcR-A protein is expressed predominantly in large imaginal discs and rings that achieve their size by cell division during metamorphosis. EcR-B1 protein is found mainly in tissues that are strictly larval and in small cell clusters that respond to ecdysone by extensive cell multiplication, such as histoblast nests and midgut imaginal islands. A temporal study of EcR-B1 protein expression through development shows that it is present from mid to late embryogenesis, is at low levels in larval stages and is higher again during pupal stages.
At the onset of metamorphosis, the EcR-A and EcR-B1 protein isoforms are expressed in most tissues and are found in approximately complimentary patterns. EcR-A protein is expressed predominantly in large imaginal discs and rings that achieve their size by cell division during metamorphosis. EcR-B1 protein is found mainly in tissues that are strictly larval and in small cell clusters that respond to ecdysone by extensive cell multiplication, such as histoblast nests and midgut imaginal islands. A temporal study of EcR-A protein expression through development shows that it is present throughout embryonic and pupal stages and is present at low levels in first instar larvae.
A 105 kD EcR protein is detected in embryos, in late third instar larvae and in early pupae. Low levels of protein are also detected in first instar larvae and adults, but no protein is detected in second and early third instar larvae. EcR protein is distributed throughout embryonic, late third instar larval, and prepupal tissues and localizes to the nucleus.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\EcR in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 114 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 39 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of EcR
Transgenic constructs containing regulatory region of EcR
Deletions and Duplications ( 15 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
gamma-lobe & neuron (with EcR31)
gamma-lobe & neuron (with EcRW53st)
glial cell & brain & pupa | conditional ts, with Scer\GAL4Tab2-201Y
microtubule & dorsal multidendritic neuron ddaC | pupal stage, with Scer\GAL4ppk.PG
neuron & ventral nerve cord (with EcR31)
neuron & ventral nerve cord (with EcR99)
nurse cell & nucleus | conditional ts
spermatid & nucleus
Tv neuron & filopodium, with Scer\GAL4FMRFa.PS
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (11)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
6 of 15
Yes
Yes
6 of 15
Yes
Yes
4 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (12)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
6 of 15
Yes
Yes
6 of 15
Yes
Yes
5 of 15
No
Yes
4 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
Rattus norvegicus (Norway rat) (12)
5 of 13
Yes
Yes
5 of 13
Yes
Yes
5 of 13
Yes
Yes
3 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (8)
5 of 12
Yes
Yes
4 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
Danio rerio (Zebrafish) (13)
6 of 15
Yes
Yes
5 of 15
No
Yes
4 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (23)
1 of 15
Yes
No
1 of 15
Yes
No
1 of 15
Yes
No
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
No
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190507 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502N6 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W06F1 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X079F )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (2)
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 1 )
Modifiers Based on Experimental Evidence ( 4 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
RNA-protein
Physical Interaction
Assay
References
RNA-RNA
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
Heterodimer of USP and ECR (PubMed:8247157). Only the heterodimer is capable of high-affinity binding to ecdysone (PubMed:8247157). Interacts with trr in an ecdysone-dependent manner (PubMed:14603321). Upon ecdysone stimulation, interacts with Nup98 (PubMed:28366641).
(UniProt, P34021 )
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2R
Recombination map
2-55
Cytogenetic map
Sequence location
2R:6,087,873..6,169,087 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
42A9-42A12
Limits computationally determined from genome sequence between P{lacW}l(2)k09848k09848&P{EP}EP407 and P{lacW}geminink14019&P{PZ}Adf101349
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
42A10-42A12
(determined by in situ hybridisation) 42A1--19 (determined by in situ hybridisation)
42A1-42A19
42A1--2 42A10--12 42A10--16
42A-42A
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (94)
Genomic Clones (61)
cDNA Clones (198)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
Other clones
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
Commercially Available Antibodies
Other Information
Relationship to Other Genes
Source for database identify of
Source for identity of: EcR CG1765
Source for database merge of
Source for merge of: snt EcR
Source for merge of: EcR CG8347
Source for merge of: EcR lie
Source for merge of: EcR anon-WO0229075.1
Additional comments
Source for merge of EcR anon-WO0229075.1 was sequence comparison ( date:051113 ).
Other Comments
Ortholog of B. mori juvenile-hormone-related gene (involved in JH biosynthesis, metabolism or signaling).
crc is an EcR receptor coactivator that is specific for the B2 isoform.
DNA-protein interactions: genome-wide binding profile assayed for EcR protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069).
The EcR-A isoform is not a signal transducer for programmed cell death in Crz-expressing ventral nerve cord neurons. EcR-B isoforms play significant roles in Crz-expressing ventral nerve cord neuron cell death.
dsRNA has been made from templates generated with primers directed against this gene. RNAi of EcR reduces the primary dendrite outgrowth of ddaD and ddaE neurons, but causes only modest reduction of lateral branching and lateral branch outgrowth. RNAi also causes defects in muscle, alterations in the number of MD neurons, defects in dendrite morphogenesis and reproducible defects in da dendrite development.
ChEST reveals this is a target of Mef2.
Ecdysone signalling cell-autonomously downregulates PI3K activity in the fat body.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
babo/Smox mediated TGFΒ/Activin signalling mediates remodeling of mushroom body neurons via controlling EcR-B1 expression.
EcR is not required for morphogenetic furrow formation in the developing eye.
The EcR/usp heterodimer DNA binding activity requires activation by a chaperone heterocomplex of six proteins including the products of Hsp83 and the Hsc70 genes. The gene products of Hsp83 and the Hsc70 genes are also required in vivo for EcR activity and EcR is the primary target of the chaperone complex.
Genetic interaction between EcRE261st and Sin3Ak07401 reported in FBrf0111507 is not evident when EcRC300Y is combined with Sin3A08269, suggesting that the original observed interaction could be due to background effects.
EcR function is required maternally for normal oogenesis.
EcR together with crol controls wing morphogenesis and cell adhesion by regulating integrin expression during metamorphosis.
EcR has a cell autonomous role in controlling neuronal remodelling.
EcR is required for hatching, at each larval moult and for the initiation of metamorphosis.
EcR/usp may play an essential role as a hormonal timer.
The ng-EcRE, located within the coding region of ng1 and ng2, binds the EcR/usp heterodimer through its widely spaced half sites.
Binding site selection procedure demonstrates that the EcR/usp heterodimer binds to the core recognition motif (consensus sequence consists of a perfect palindrome of the heptameric half-site sequence GAGGTCA that is separated by a single A/T base pair).
Mutations in EcR reveal functional differences among the receptor isoforms encoded by the EcR gene.
Identified in a screen for modifiers of the Dfd13/Dfd3 mutant phenotype. Mutants weakly interact with Pc.
The EcR/usp heterodimer binds in vitro to direct repeats and these repeats can confer ecdysteroid responsiveness on minimal promoters in a cell transfection assay. The natural pseudopalindromic EcR/usp binding site in the Fbp1 enhancer is required to mediate a fat body-specific ecdysteroid response. The profound differences in structure between these two types of EcR/usp binding site do not dictate a spatial and temporal specificity of the transcriptional response they mediate.
Interaction of the DNA binding domain with a 20-hydroxyecdysone palindromic response element from the promoter region of Hsp27 has been studied.
DNA-blotting assay has identified a high affinity ecdysone receptor binding site within the ng1 and ng2 coding sequence. EMSA assay demonstrates the 93bp 'ng element' is able to bind an EcR/usp heterodimer and usp alone.
Expression throughout the onset of metamorphosis is not affected by Eip74EF mutations.
EcR and usp interact as a dimer.
The usp product acts as an allosteric effector to enhance steroid binding by the EcR product. Under some conditions, DNA binding by EcR/usp product is highly steroid-dependent.
The EcR product binds to two sites, element I and element II, in the regulatory region of Sgs4. Element II appears to be of no importance for the expression of Sgs4 while element I is an ecdysone response element necessary, but not sufficient, for Sgs4 induction.
EcR has been stably transfected into CHO cells. Nuclear extracts from the line exhibit specific binding activity for the Hsp27 ecdysone response element in mobility shift analyses.
The in vitro characterisation of the EcR/usp binding site its ecdysone- dependent in vivo occupancy in different genetic backgrounds support the conclusion that the Fbp1 enhancer is a primary target of the EcR.
Neurons show qualitative and quantitative changes in EcR expression during their life history and these differences correlate with distinct patterns of ecdysteroid response.
Ecdysteroid-regulated gene.
Postmeiotic differentiation defect.
The correlation of a unique pattern of ecdysone receptor isoform A expression in the CNS of the emerging adult with a particular steroid regulated cell death fate suggests that variations in the pattern of receptor isoform expression may serve as important switches during development.
The ecdysone receptor gene encodes three receptor isoforms with common DNA and hormone binding domains but different N-terminal regions. Different isoforms predominate at different developmental stages that are marked by a pulse of ecdysone.
Transient cotransfection experiments in HeLa cells demonstrated that EcR must heterodimerize with usp (the homolog of the mammalian retinoid X receptor) for DNA binding and transactivation. EcR/usp gene product DNA binding activity is unaffected by ecdysteroid and 9-cis-retinoic acid.
EcR and usp native gene products co-localise on ecdysone-responsive loci. Physical associations in the presence and absence of ecdysone redefine the ecdysone receptor as a dynamic complex whose activity may be altered by combinatorial interactions among subunits and ligand.
EcR protein can function as a ligand-dependent transcription factor in mammalian cells.
Cotransfection of both usp and EcR is required to render cultured mammalian cells ecdysone responsive.
usp is a Drosophila partner of EcR. Together usp and EcR bind DNA in a highly cooperative pattern.
Identification: EcR was identified in a screen for members of the steroid receptor superfamily.
The DNA binding properties of the ecdysone receptor protein have been defined. In vitro binding studies demonstrate that ecdysterone causes activation or repression of the receptor DNA binding domain via an irreversible change in conformation.
EcR binds as a dimer to an imperfect palindromic sequence (GGTTCAATGCACT) in the Hsp27 promoter region.
The effect of pyridoxal 5'-phosphate on the binding of EcR from a nuclear extract to DNA-cellulose is studied: application inhibits EcR binding to DNA.
A novel bromoacetly ecdysteroid IV reacts with partially purified EcR rapidly and almost quantitatively.
Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 114 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
Other crossreferences
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
KEGG Genes - Molecular building blocks of life in the genomic space.
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
modMine - A data warehouse for the modENCODE project
SignaLink - A signaling pathway resource with multi-layered regulatory networks.
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyMine - An integrated database for Drosophila genomics
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Synonyms and Secondary IDs (44)
Reported As
Symbol Synonym
Dhr23
EcR
(Ke and Hsu, 2019, Lee et al., 2019, Meltzer et al., 2019, Moreno et al., 2019, Pahl et al., 2019, Shokri et al., 2019, Xu et al., 2019, Zheng et al., 2019, Zhu et al., 2019, Ameku et al., 2018, Baron et al., 2018, Bischof et al., 2018, Bohère et al., 2018, Bovier et al., 2018, Buhler et al., 2018, Gáliková and Klepsatel, 2018, Gene Disruption Project members, 2018-, Geronikolou et al., 2018, Jiang et al., 2018, Lee et al., 2018, Lehmann, 2018, Lim et al., 2018, Liu et al., 2018, Meiselman et al., 2018, Sharma et al., 2018, Zou et al., 2018, Ables and Drummond-Barbosa, 2017, Busto et al., 2017, Doe, 2017, He et al., 2017, Herzmann et al., 2017, Jia et al., 2017, Kang et al., 2017, Kreher et al., 2017, Manning et al., 2017, Mazina et al., 2017, Misra et al., 2017, Pascual-Garcia et al., 2017, Rohde et al., 2017, Takayanagi-Kiya et al., 2017, Tomita et al., 2017, Transgenic RNAi Project members, 2017-, Zheng et al., 2017, Brookheart and Duncan, 2016, Chiang et al., 2016, Clandinin and 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anon-WO0229075.1
ms(2)06410
Name Synonyms
Ecdyson Receptor 1b
Ecdysone-R
ecdysone receptor complex
ecdysone-Receptor
ecdysterone receptor
long island expressway
male sterile(2)42A
snaggletooth
Secondary FlyBase IDs
  • FBgn0011242
  • FBgn0021762
  • FBgn0033064
  • FBgn0062534
Datasets (1)
Study focus (1)
Experimental Role
Project
Project Type
Title
  • transgene_used
Protein profiling reveals five principal chromatin types in Drosophila cells.
References (860)