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General Information
Symbol
Dmel\EcR
Species
D. melanogaster
Name
Ecdysone receptor
Annotation Symbol
CG1765
Feature Type
FlyBase ID
FBgn0000546
Gene Model Status
Stock Availability
Gene Snapshot
Ecdysone receptor (EcR) encodes a protein that interacts with the product of usp to form the nuclear ecdysone receptor heterodimer, which modulates, in conjunction with co-activators and co-repressors, the activities of hundreds of genes in a tissue- and stage-specific way. EcR is widely expressed in embryonic and larval tissues and in some adult tissues where its activities (modulated by the hormone ecdysone) trigger both molting and metamorphosis. [Date last reviewed: 2019-03-07]
Also Known As

EcR-B1, EcRB1, DmEcR, EcR-A, ecdysteroid receptor

Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:6,087,873..6,169,087 [-]
Recombination map

2-55

RefSeq locus
NT_033778 REGION:6087873..6169087
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (79 terms)
Molecular Function (16 terms)
Terms Based on Experimental Evidence (11 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from physical interaction with FLYBASE:usp; FB:FBgn0003964
inferred from physical interaction with FLYBASE:Utx; FB:FBgn0260749
inferred from physical interaction with FLYBASE:rig; FB:FBgn0250850
inferred from physical interaction with UniProtKB:Q9VCH5
(assigned by UniProt )
Terms Based on Predictions or Assertions (8 terms)
CV Term
Evidence
References
non-traceable author statement
inferred from sequence or structural similarity with MGI:MGI:1352464
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN001182089
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001182089
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001182089
(assigned by GO_Central )
non-traceable author statement
inferred from electronic annotation with InterPro:IPR001723
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN001182089
(assigned by GO_Central )
inferred from electronic annotation with InterPro:IPR001628, InterPro:IPR013088
(assigned by InterPro )
Biological Process (55 terms)
Terms Based on Experimental Evidence (47 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:if; FB:FBgn0001250
inferred from genetic interaction with FLYBASE:mys; FB:FBgn0004657
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:babo; FB:FBgn0011300
inferred from genetic interaction with FLYBASE:Smox; FB:FBgn0025800
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:InR; FB:FBgn0283499
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (12 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001182089
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000637752
(assigned by GO_Central )
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000637752
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000637752
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001182089
(assigned by GO_Central )
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN001182089
(assigned by GO_Central )
Cellular Component (8 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN001182089
(assigned by GO_Central )
Protein Family (UniProt)
Belongs to the nuclear hormone receptor family. NR1 subfamily. (P34021)
Summaries
Gene Group (FlyBase)
NUCLEAR RECEPTOR (LIGAND-DEPENDENT) TRANSCRIPTION FACTORS -
Nuclear receptors (NR) are C4 zinc finger ligand-dependent DNA-binding transcription factors. Members of the NR superfamily are defined by the presence of a highly conserved DNA-binding domain and a less conserved C-terminal ligand-binding domain. (Adapted from FBrf0184203).
Protein Function (UniProtKB)
Receptor for ecdysone (PubMed:1913820, PubMed:30293839). Binds to ecdysone response elements (ECRES) following ecdysone-binding, and recruitment of a complex containing the histone methyltransferase trr, leads to activate transcription of target genes (PubMed:1913820, PubMed:30293839).
(UniProt, P34021)
Summary (Interactive Fly)

transcription factor - nuclear receptor - zinc finger - regulates molting cycles and oogenesis

Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
3

Please see the GBrowse view of Dmel\EcR or the JBrowse view of Dmel\EcR for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0086008
5108
849
FBtr0086011
4092
878
FBtr0086012
4984
669
FBtr0086009
3740
849
FBtr0086010
5233
849
FBtr0302439
8415
878
Additional Transcript Data and Comments
Reported size (kB)

6, 5, 4 (northern blot)

6.0 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0085349
91.2
849
7.71
FBpp0085352
93.9
878
6.88
FBpp0085353
73.3
669
6.76
FBpp0085350
91.2
849
7.71
FBpp0085351
91.2
849
7.71
FBpp0291631
93.9
878
6.88
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

849 aa isoforms: EcR-PA, EcR-PD, EcR-PE
878 aa isoforms: EcR-PB, EcR-PG
Additional Polypeptide Data and Comments
Reported size (kDa)

878, 849, 669 (aa); 105, 80 (kD)

878 (aa); 105 (kD observed)

Comments

alternatively spliced exon

External Data
Subunit Structure (UniProtKB)

Heterodimer of USP and ECR (PubMed:8247157). Only the heterodimer is capable of high-affinity binding to ecdysone (PubMed:8247157). Interacts with trr in an ecdysone-dependent manner (PubMed:14603321). Upon ecdysone stimulation, interacts with Nup98 (PubMed:28366641).

(UniProt, P34021)
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\EcR using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference

Comment: reference states 0-3 hr AEL

in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference

Comment: reference states >=3 hr AEL

Additional Descriptive Data

The temporal pattern of EcR RNA expression was carefully studied and related to times of ecdysone pulses. Peaks of the 5kb EcR-A RNA occur in mid-embryogenesis and early and late in pupation.

The temporal pattern of EcR RNA expression was carefully studied and related to times of ecdysone pulses. The strongest peak of EcR-B1 RNA expression occurs in late third instar larvae. Smaller peaks are observed during late embryonic, late first and second instar larval, and mid pupal stages.

The 6 kb EcR transcript is detected in all stages except for 0-3 hr embryos. Expression is most abundant in embryos and in late third instar larvae and early pupae, with highest levels seen in wandering third instar larvae.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
neuron | subset

Comment: reference states >=12 hr APF

ventral nervous system | restricted

Comment: reference states >=12 hr APF

western blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

At 0h APF, reticular neuropil associated glial cells (astrocyte-like cells) express "EcR-B1" (EcR-RB), but not "EcR-A" (EcR-RA, EcR-RD) <up>isoform identities obtained from UniProt</up>.

EcR staining is widespread in the adult brain.

EcR isoform "A" is uniformly distributed in egg chambers at all stages. Isoform "B1" is more highly expressed in follicle cells than germline cells and shows a fourfold enrichment in anterior follicle cells at early oogenesis stage S9. The enrichment is reduced by mid stage 9 and absent by stage 10.

EcR is expressed in approximately 20 antennal lobe projection neurons, 18 of which are located in a anterodorsal cluster.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
Reporter: P{EcR.GET-BD-GAL4}
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\EcR in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 114 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 43 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of EcR
Transgenic constructs containing regulatory region of EcR
Deletions and Duplications ( 15 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
gamma-lobe & neuron (with EcR31)
gamma-lobe & neuron (with EcRW53st)
glial cell & brain & pupa | conditional ts, with Scer\GAL4Tab2-201Y
microtubule & dorsal multidendritic neuron ddaC | pupal stage, with Scer\GAL4ppk.PG
neuron & ventral nerve cord (with EcR31)
neuron & ventral nerve cord (with EcR99)
nurse cell & nucleus | conditional ts
spermatid & nucleus
Tv neuron & filopodium, with Scer\GAL4FMRFa.PS
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (17)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
10 of 15
Yes
Yes
1  
9 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
1  
1 of 15
No
No
1 of 15
No
No
1  
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (18)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
9 of 15
Yes
Yes
9 of 15
Yes
Yes
5 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (18)
8 of 13
Yes
Yes
8 of 13
Yes
Yes
5 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
No
2 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (15)
9 of 12
Yes
Yes
6 of 12
No
Yes
2 of 12
No
No
2 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (25)
8 of 15
Yes
Yes
4 of 15
No
Yes
4 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (191)
3 of 15
Yes
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
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Yes
1 of 15
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Yes
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Yes
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Yes
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Yes
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Yes
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Yes
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Yes
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Yes
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Yes
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Yes
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Yes
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Yes
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Yes
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Yes
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Yes