FlyBase Gene Report: Dmel\Edg78E

General Information

Symbol

Dmel\Edg78E

Species

D. melanogaster

Name

Ecdysone-dependent gene 78E

Annotation Symbol

CG7673

Feature Type

protein_coding_gene

FlyBase ID

FBgn0000551

Gene Model Status

Current

Stock Availability

2 publicly available

Gene Summary

Ecdysone-dependent gene 78E (Edg78E) encodes a pupal cuticle protein that is induced by ecdysone during prepupal development. It is only found in the pupal exocuticle where it binds chitin and serves as part of the structural basis of the cuticle. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

EDG78

Key Links

Genomic Location

Cytogenetic map

78C6-78C6

Sequence location

3L:21,296,763..21,297,803 [-]

Recombination map

3-47

RefSeq locus

NT_037436 REGION:21296763..21297803

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (4 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables structural constituent of chitin-based larval cuticle

inferred from biological aspect of ancestor with PANTHER:PTN002571557

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence model

(Karouzou et al., 2007)

enables structural constituent of pupal chitin-based cuticle

traceable author statement

(Andres et al., 1993, Apple and Fristrom, 1991)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in chitin-based cuticle development

inferred from sequence model

(Karouzou et al., 2007)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in chitin-based extracellular matrix

inferred from biological aspect of ancestor with PANTHER:PTN002571557

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in chitin-based extracellular matrix

inferred from sequence model

(Karouzou et al., 2007)

Gene Group (FlyBase)

CPR CUTICLE PROTEIN FAMILY

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

CPR CUTICLE PROTEIN FAMILY -: The largest cuticle protein family, the CPR family, contain a 35 amino acid R&R consensus (named after Rebers and Riddiford, PMID:2462055), which in an extended form has been shown to bind chitin (PMID:15475300). (Adapted from FBrf0242484.)

Protein Function (UniProtKB)

Component of the cuticle of the pupa of fruit fly.

(UniProt, P27779)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Edg78E for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P27779)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.45

Gene model reviewed during 5.54

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Edg78E-RA

FBtr0078408

NM_079474

967

122

Edg78E-RB

FBtr0344634

NM_001300211

546

122

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Edg78E-PA

FBpp0078062

13.3

122

4.48

NP_524198

AAF51702

Edg78E-PB

FBpp0310951

13.3

122

4.48

NP_001287140

AHN58165

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

122 aa isoforms: Edg78E-PA, Edg78E-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Edg78E using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Edg78E_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

prepupal stage

epidermis

(Fechtel et al., 1989)

Additional Descriptive Data

Edg78E transcripts are present in equal abundance in imaginal and larval epidermis. Transcripts are detected in 8hr pupae and reach maximal accumulation in 10hr prepupae. None are detectable in 0-6hr prepupae or 14hr pupae.

(Fechtel et al., 1989)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Edg78E in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Edg78E

View exonic expression by tissue for Dmel\Edg78E

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40354/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0000551

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0000551

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0000551

Flygut - An atlas of the Drosophila adult midgut

FBgn0000551

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Edg78E

Transgenic constructs containing regulatory region of Edg78E

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Edg78E^GD6772

viable, with Scer\GAL4^Mef2.PR

Edg78E^GD6772

viable, with Scer\GAL4^pnr-MD237

Edg78E^GD6772

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (0)

No records found.

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (0)

No records found.

Rattus norvegicus (Norway rat) (0)

No records found.

Xenopus tropicalis (Western clawed frog) (0)

No records found.

Danio rerio (Zebrafish) (0)

No records found.

Caenorhabditis elegans (Nematode, roundworm) (0)

No records found.

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (62)

Gene Symbol

Score

Source

Alignment

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

Pcp

3 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

65602

DroID - A comprehensive database of gene and protein interactions.

FBgn0000551

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-47

Cytogenetic map

78C6-78C6

Sequence location

3L:21,296,763..21,297,803 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

78C6-78C6

Limits computationally determined from genome sequence between P{lacW}l(3)j1B10^j1B10 and P{lacW}l(3)j2C4^j2C4

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

78E-78E

(determined by in situ hybridisation)

(Fechtel et al., 1988)

Experimentally Determined Recombination Data

Location

3-47

(FlyBase, 2016)

3-43.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (2)

Aberrations including deletions of this gene Aberrations including duplications of this gene

VDRC

v107367

P{KK106712}VIE-260B

v31133

w¹¹¹⁸; P{GD6772}v31133

Genomic Clones (9)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (2)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI14131

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0000551

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

LP03489

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0000551

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40354

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Additional comments

Other Comments

In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.

(Kliman and Eyre-Walker, 1998)

Temporal profile of gene expression is altered in Eip74EF mutant background.

(Fletcher and Thummel, 1995)

Premature expression of ftz-f1 induces the Edg78E and Edg84A genes which have strong ftz-f1 binding sites upstream of their transcription start sites.

(Ueda et al., 1995)

Ecdysteroid-regulated gene.

(Andres et al., 1993)

Initial characterization of Edg78E suggests the gene encodes a cuticle protein.

(Apple and Fristrom, 1991)

The spatial and temporal expression is as expected for genes that encode pupal cuticle proteins.

(Fechtel et al., 1989)

mRNA on Northerns: 0.6 kb Identification: Differential screen of a genomic library with cDNA isolated from imaginal discs pulsed with 20-hydroxyecdysone, 20-HE (Fechtel, Natzle, Brown and Fristrom, 1988). Transcripts in imaginal disc tissue, beginning at six to nine hours, following a six-hour 20-HE pulse, but not in the absence or continual presence of 20-HE; mRNA's enriched in membrane-bound polysome fractions of imaginal discs. Translation product from hybrid selected mRNA of Edg78E reacts with polyclonal antibodies prepared against purified pupal cuticle proteins.

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 44 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40354

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P27779

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P27779

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

X2JGZ5

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P27779

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0000551

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40354/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0000551

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Edg78E_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0000551

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0000551

Flygut - An atlas of the Drosophila adult midgut

FBgn0000551

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40354

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG7673

MARRVEL_MODEL - MARRVEL (model organism gene)

40354

modMine - A data warehouse for the modENCODE project

FBgn0000551

Linkouts

BioGRID - A database of protein and genetic interactions.

65602

DroID - A comprehensive database of gene and protein interactions.

FBgn0000551

DRSC - Results frm RNAi screens

FBgn0000551

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0000551

FlyMine - An integrated database for Drosophila genomics

FBgn0000551

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

CG7673

(Hosono et al., 2015, Keleman et al., 2009.8.5)

DMLCP78

(Iconomidou et al., 1999)

DmelEdg78E

(Karouzou et al., 2007)

EDG-78

(Fristrom and Fristrom, 1993)

EDG78

(Andersen et al., 1995, Ueda et al., 1995, Apple and Fristrom, 1991)

EDG78E

(Zhou and Riddiford, 2001, Kawasaki et al., 1998)

Edg78

(Bayer et al., 1996)

Edg78E

(Yue et al., 2020, Xie et al., 2015, Vanden Broeck et al., 2013, Blanco et al., 2010, Blanco et al., 2008, Zhou and Riddiford, 2008, Karouzou et al., 2007, Karouzou et al., 2007, Willis, 2007.1.3, Beckstead et al., 2005, Kawasaki et al., 2002)

Edg78e

(Rifkin et al., 2003)

Name Synonyms

Ecdysone-dependent gene 78E

Secondary FlyBase IDs

Datasets (4)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (4)