FB2019_05, released Oct 15, 2019

Gene: Dmel\Eh

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General Information
Symbol
Dmel\Eh
Species
D. melanogaster
Name
Eclosion hormone
Annotation Symbol
CG5400
Feature Type
protein_coding_gene
FlyBase ID
FBgn0000564
Gene Model Status
Current
Stock Availability
5 publicly available
Gene Snapshot
Eclosion hormone (Eh) encodes a neurohormone involved in the control of ecdysis. At each ecdysis, the neurohormone is released into the hemolymph where it serves to potentiate the release of the product of ETH through a positive endocrine feedback loop. The product of Eh is also released into the nervous system where it contributes to turning on ecdysis. [Date last reviewed: 2018-09-13]
Other Summaries
Also Known As
DMEHAB, Drm-EH
Key Links
Genomic Location
Cytogenetic map
90B1-90B1
Sequence location
3R:17,446,642..17,448,858 [-]
Recombination map
3-61
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the insect eclosion hormone family. (Q07892)
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
-
Predictions / Assertions
ecdysis-triggering hormone activity; eclosion hormone activity; neuropeptide hormone activity
Summaries
Gene Group (FlyBase)
NEUROPEPTIDES -
Neuropeptides are secreted into the extracellular space where they interact with cell surface receptors (usually G protein coupled receptors). They are extremely diverse, acting as neurotransmitters, neuromodulators, hormones or growth factors. (Adapted from FBrf0211443 and PMID:27813667).
Protein Function (UniProtKB)
Neuropeptide that triggers the performance of ecdysis behaviors at the end of a molt. It triggers adult behavior patterns: larval, pupal and adult ecdysis, and plasticization during the molt.
(UniProt, Q07892)
Summary (Interactive Fly)
neuropeptide - acts directly on the CNS to release the stereotyped motor programs that trigger the shedding of the old cuticle at the end of each molt
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\Eh or the JBrowse view of Dmel\Eh for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.47
Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Eh-RA
FBtr0083444
NM_079662
704
97
Eh-RB
FBtr0334930
NM_001275739
700
97
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Eh-PA
FBpp0082885
10.6
97
7.22
NP_524386
AAF55423
Eh-PB
FBpp0306950
10.6
97
7.22
NP_001262668
AGB96048
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

97 aa isoforms: Eh-PA, Eh-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Eh using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (8 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
ecdysis-triggering hormone activity
inferred from electronic annotation with InterPro:IPR006825
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
eclosion hormone activity
non-traceable author statement
(Hewes and Taghert, 2001)
inferred from sequence or structural similarity
(Horodyski et al., 1993)
neuropeptide hormone activity
traceable author statement
(vanden Broeck, 2001)
Biological Process (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
ecdysis, chitin-based cuticle
inferred from mutant phenotype
(Krüger et al., 2015)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
eclosion
traceable author statement
(vanden Broeck, 2001)
neuropeptide signaling pathway
inferred from electronic annotation with InterPro:IPR006825
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
traceable author statement
(vanden Broeck, 2001)
regulation of eclosion
inferred from sequence or structural similarity
(Horodyski et al., 1993)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
extracellular space
inferred from sequence model
(Horodyski et al., 1993)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 13 -- 16
embryonic central brain neuron
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Eh in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 8 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Eh
Transgenic constructs containing regulatory region of Eh
Deletions and Duplications ( 1 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal (with Df(3R)Eh)
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
Other Phenotypes
Allele
majority die during larval stage (with Df(3R)Eh)
Phenotype manifest in
Allele
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (0)
No records found.
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (0)
No records found.
Rattus norvegicus (Norway rat) (0)
No records found.
Xenopus tropicalis (Western clawed frog) (0)
No records found.
Danio rerio (Zebrafish) (0)
No records found.
Caenorhabditis elegans (Nematode, roundworm) (4)
Cele\clec-251
1 of 15
Yes
Yes
Cele\clec-253
1 of 15
Yes
Yes
Cele\clec-256
1 of 15
Yes
Yes
Cele\F11D11.6
1 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190JZL )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\Eh
Drosophila simulans
Dsim\Eh
Drosophila sechellia
Dsec\Eh
Drosophila erecta
Dere\GG16772
Drosophila yakuba
Dyak\GE25269
Drosophila ananassae
Dana\GF18611
Drosophila pseudoobscura pseudoobscura
Dpse\GA18853
Drosophila persimilis
Dper\GL23932
Drosophila willistoni
Dwil\GK11451
Drosophila virilis
Dvir\GJ22822
Drosophila mojavensis
Dmoj\GI22821
Drosophila grimshawi
Dgri\GH17491
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150I4C )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17019751
Glossina morsitans
Tsetse fly
Gmor\GMOY012086
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_02039
Mayetiola destructor
Hessian fly
Mdes\Mdes009519
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL011228
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL011229
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL015552
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL006722
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL014409
Anopheles darlingi
American malaria mosquito
Adar\ADAC002990
Anopheles gambiae
Malaria mosquito
Agam\ECH
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ013610
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ013611
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ011911
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0Q0Q )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA006291
Danaus plexippus
Monarch butterfly
Dple\DPOGS213566-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS210673-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL007691
Heliconius melpomene
Postman butterfly
Hmel\HMEL009449
Heliconius melpomene
Postman butterfly
Hmel\HMEL017369
Apis florea
Little honeybee
Aflo\2722210532
Apis mellifera
Western honey bee
Amel\GB49648
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP17811
Bombus terrestris
Buff-tailed bumblebee
Bter\15216963
Linepithema humile
Argentine ant
Lhum\LH19695
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0021ef
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG003184
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_02807
Tribolium castaneum
Red flour beetle
Tcas\Eh
Pediculus humanus
Human body louse
Phum\PHUM580410
Rhodnius prolixus
Kissing bug
Rpro\RPRC014242
Cimex lectularius
Bed bug
Clec\CLEC002327
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI007357
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI42083
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI085492
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_16998
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0TO3 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR011657
Strigamia maritima
European centipede
Smar\SMAR011656
Ixodes scapularis
Black-legged tick
Isca\ISCW001941
Stegodyphus mimosarum
African social velvet spider
Smim\KFM67953.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM81769.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM77838.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM81770.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur08g04650
Tetranychus urticae
Two-spotted spider mite
Turt\tetur11g03800
Tetranychus urticae
Two-spotted spider mite
Turt\tetur02g09290
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_240157
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_48309
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Interactions Browser
    Summary of Genetic Interactions
    esyN Network Diagram
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map
    3-61
    Cytogenetic map
    90B1-90B1
    Sequence location
    3R:17,446,642..17,448,858 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    90B1-90B1
    Limits computationally determined from genome sequence between P{EP}CG5208EP562&P{EP}DadEP3196 and P{PZ}l(3)0788207882
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    90B1-90B2
    (determined by in situ hybridisation)
    (Horodyski et al., 1993, Horodyski, 1992)
    Experimentally Determined Recombination Data
    Location
    3-61
    (FlyBase, 2016)
    3-59.6
    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (5)
    Genomic Clones (31)
    cDNA Clones (3)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of
        Source for database merge of
        Additional comments
        Other Comments
        In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
        (Kliman and Eyre-Walker, 1998)
        Isolation and characterisation of Eh.
        (Horodyski et al., 1993)
        Eh gene structure and expression has been characterised.
        (Horodyski et al., 1990)
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 27 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyMine - An integrated database for Drosophila genomics
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        Synonyms and Secondary IDs (9)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (82)