FlyBase Gene Report: Dmel\Eip74EF

General Information

Symbol

Dmel\Eip74EF

Species

D. melanogaster

Name

Ecdysone-induced protein 74EF

Annotation Symbol

CG32180

Feature Type

protein_coding_gene

FlyBase ID

FBgn0000567

Gene Model Status

Current

Stock Availability

56 publicly available

Gene Summary

Ecdysone-induced protein 74EF (Eip74EF) encodes a transcription factor that responds to different concentrations of 20-hydroxyecdysone. It contributes to puparium formation and autophagy. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Red Book
Interactive Fly

Also Known As

E74, E74A, E74B, Eip74, ECIP

Key Links

Genomic Location

Cytogenetic map

74D4-74E2

Sequence location

3L:17,558,672..17,619,325 [-]

Recombination map

3-45

RefSeq locus

NT_037436 REGION:17558672..17619325

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (14 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables DNA-binding transcription activator activity, RNA polymerase II-specific

inferred from direct assay

(Urness and Thummel, 1995)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables DNA-binding transcription factor activity, RNA polymerase II-specific

inferred from biological aspect of ancestor with PANTHER:PTN000218930

(GO Reference Genome Project, 2011-)

enables DNA-binding transcription factor activity

inferred from electronic annotation with InterPro:IPR000418, InterPro:IPR046328

(InterPro Project Members, 2004-)

enables sequence-specific DNA binding

inferred from electronic annotation with InterPro:IPR000418

(InterPro Project Members, 2004-)

Biological Process (9 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in autophagic cell death

inferred from mutant phenotype

(Jiang et al., 2000)

involved_in autophagy

inferred from mutant phenotype

(Lee and Baehrecke, 2001)

involved_in positive regulation of transcription by RNA polymerase II

inferred from mutant phenotype

(Urness and Thummel, 1995)

involved_in spermatogenesis

inferred from mutant phenotype

(Chebbo et al., 2021)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in cell differentiation

inferred from biological aspect of ancestor with PANTHER:PTN000218930

(GO Reference Genome Project, 2011-)

involved_in oogenesis

non-traceable author statement

(Kozlova and Thummel, 2000)

involved_in regulation of development, heterochronic

traceable author statement

(Thummel, 2001)

involved_in regulation of DNA-templated transcription

inferred from electronic annotation with InterPro:IPR000418

(InterPro Project Members, 2004-)

involved_in regulation of transcription by RNA polymerase II

inferred from electronic annotation with InterPro:IPR046328

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000218930

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Ihry and Bashirullah, 2014, Burtis et al., 1990)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000218930

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

ETS DOMAIN TRANSCRIPTION FACTORS

Protein Family (UniProt)

Belongs to the ETS family. (P11536 P20105)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

ETS DOMAIN TRANSCRIPTION FACTORS -: The E26 transformation specific (ETS) domain transcription factors are sequence-specific DNA-binding proteins that regulate transcription. These factors are characterized by a conserved 85 amino acid DNA binding ETS domain that binds specifically to purine-rich DNA motif GGAA/T. (Adapted from PMID:11715049 and PMID:9570133).

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

E74: Ecdysone-inducible gene encoded in region 74 (W.A. Segraves)

Encodes an ecdysone-inducible gene associated with the early puff at 74E-F. Periodic expression of the gene follows, by approximately one hour, pulses of ecdysone occurring during development; two periods of expression occur independently of ecdysone pulses, one at the end of embryogenesis and one at the end of pupal development. Return to base line levels of expression requires protein synthesis and presumably results from repression by ecdysone-induced gene products; in situ hybridization to larvae detects transcription of E74 in most tissues, both imaginal and strictly larval.

Summary (Interactive Fly)

ETS domain transcription factor that encodes two isoforms that control the timing of gene induction during molting

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Eip74EF for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P11536)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Unconventional translation start (CUG) postulated; FBrf0051390, FBrf0064374.

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Eip74EF-RA

FBtr0075202

NM_168740

6012

829

Eip74EF-RB

FBtr0075204

NM_168741

5059

883

Eip74EF-RC

FBtr0075203

NM_168739

6012

868

Eip74EF-RD

FBtr0100411

NM_001014590

6239

883

Eip74EF-RE

FBtr0305796

NM_001259887

6012

844

Additional Transcript Data and Comments

Reported size (kB)

6.0 (northern blot)

(Chen et al., 1992)

6.0, 5.1, 4.8 (northern blot)

(Burtis et al., 1990)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Eip74EF-PA

FBpp0074965

87.1

829

6.53

FBpp0074967

94.8

883

6.90

FBpp0074966

91.3

868

6.48

FBpp0099825

94.8

883

6.90

FBpp0297063

88.5

844

6.53

M9NED0

NP_001246816

AFH04487

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

883 aa isoforms: Eip74EF-PB, Eip74EF-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

883 (aa)

(Lautenberger et al., 1992)

883, 829 (aa); 94.7, 87.1 (kD predicted)

(Burtis et al., 1990)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Eip74EF using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.82

Transcript Expression

dissected tissue

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval salivary gland

Comment: from -12 hr APF

(Ihry and Bashirullah, 2014)

prepupal stage P4

embryonic/larval salivary gland

(Ihry and Bashirullah, 2014)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 15 -- 17

organism

Comment: 12-24 hr AEL

(Chen et al., 1992)

first instar larval stage

organism | faint

(Chen et al., 1992)

late third instar larval stage

organism

(Chen et al., 1992)

embryonic/larval salivary gland

Comment: 4 hr before puparium formation

(Lehmann et al., 2002)

wandering third instar larval stage

organism

(Ritter and Beckstead, 2010)

prepupal stage

(Bashirullah et al., 2003)

organism | faint

(Chen et al., 1992)

prepupal stage P1 -- P3

organism

Comment: 0-6 hr APF

(Bashirullah et al., 2003)

prepupal stage P1 -- prepupal stage P4(ii)

organism

(Ritter and Beckstead, 2010)

prepupal stage P4

organism

Comment: 10-12 hr APF

(Bashirullah et al., 2003)

prepupal stage P4(ii)

embryonic/larval salivary gland

Comment: 12 hr APF

(Lehmann et al., 2002)

pupal stage

organism

Comment: Assay specific to Eip74EF transcripts Eip74EF-RA (E74A) and Eip74EF-RB (E74B).

(Liu et al., 2012)

pupal stage P5

embryonic/larval salivary gland

(Cao et al., 2007)

day 1 of adulthood

organism | faint

(Chen et al., 1992)

day 2 of adulthood

organism

(Chen et al., 1992)

day 3 of adulthood | male

adult head

Comment: Assay specific to Eip74EF transcript Eip74EF-RA (E74A) and Eip74EF-RB (E74B). Only Eip74EF-RA is expressed.

(Liu et al., 2012)

day 30 of adulthood | male

adult head

Comment: Assay specific to Eip74EF transcript Eip74EF-RA (E74A).

(Liu et al., 2012)

Additional Descriptive Data

Expression increases after the late larval (-4 hr APF) and prepupal (+10 hr APF) ecdysone pulses.

(Ihry and Bashirullah, 2014)

Eip74EF transcript Eip74EF-RA is expressed in pupa and adult males. Transcript Eip74EF-RB is absent from adult males.

(Liu et al., 2012)

Expression of Eip74EF coincides with a pulse of ecdysone shortly before puparium formation, and a second wave at 10 hours after pupation.

(Ritter and Beckstead, 2010)

Eip74EF transcript is strongly expressed in salivary glands at the late larval and late prepupal ecdysone pulses.

(Lehmann et al., 2002)

RNA blots were carried out on RNA extracted from staged larval and prepupal salivary glands. Eip74EF transcripts are induced in apparent response to the late-larval and prepupal ecdysone pulses paralleling the puffing response at 74EF. This response is enhanced in response to ectopic ftz-f1 expression.

(Woodard et al., 1994)

Peaks of Eip74EF expression are seen in late embryos, late third instar larvae, and mid pupae.

(Chen et al., 1992)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

day 0 of adulthood | male

adult head | intense

Comment: Adults younger than 12h. Assay specific to Eip74EF isoform Eip74EF-PA (E74A).

(Liu et al., 2012)

day 1 of adulthood | male

adult head

Comment: Assay specific to Eip74EF isoform Eip74EF-PA (E74A).

(Liu et al., 2012)

day 3 of adulthood -- day 60 of adulthood & male

adult head | faint

Comment: Assay specific to Eip74EF isoform Eip74EF-PA (E74A).

(Liu et al., 2012)

Additional Descriptive Data

Eip74EF protein isoform Eip74EF-PA is strongly expressed in the head of adults younger than 12h. Expression decreases rapidly and from day 1 onwards, weak expression is observed.

(Liu et al., 2012)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Ihry and Bashirullah, 2014, Burtis et al., 1990)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Eip74EF in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Eip74EF

View exonic expression by tissue for Dmel\Eip74EF

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0000567

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39962/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0000567

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0000567

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0000567

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG32180

Flygut - An atlas of the Drosophila adult midgut

FBgn0000567

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 41 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Eip74EF

Transgenic constructs containing regulatory region of Eip74EF

Aberrations (Deficiencies and Duplications) ( 8 )

Inferred from experimentation ( 8 )

Gene partially disrupted in

Df(3R)ED4688

(Keleman et al., 2005)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Eip74EF²

lethal | recessive

lethal - all die before end of larval stage

Eip74EF^DL-1

lethal - all die before end of P-stage

Eip74EF^neo24

lethal - all die before end of P-stage | recessive

Eip74EF¹

viable

Eip74EF^BG01805

Other Phenotypes

Allele

abnormal neuroanatomy | heat sensitive

Eip74EF^A.hs

decreased cell death

Eip74EF^neo24

short lived | heat sensitive

Eip74EF^A.hs

some die during larval stage

Eip74EF^DL-1

some die during pharate adult stage

Eip74EF^neo24

some die during pharate adult stage | recessive

Eip74EF¹

some die during P-stage

Eip74EF^neo24

Phenotype manifest in

Allele

adult brain | heat sensitive

Eip74EF^A.hs

adult epidermis

Eip74EF^neo24

air bubble

Eip74EF^DL-1

anterior pupal spiracle

Eip74EF^DL-1

cuticle

Eip74EF²

embryonic/larval midgut | prepupal stage (with Df(3L)81k19)

embryonic/larval salivary gland

Eip74EF^neo24

embryonic/larval salivary gland | embryonic stage

Eip74EF^neo24

larva

Eip74EF²

procephalic segment

Eip74EF^DL-1

salivary gland

Eip74EF^neo24

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (29)

Hsap\ELF2

7 of 14

Yes

Hsap\ELF4

5 of 14

Yes

Hsap\ELF1

4 of 14

Yes

Hsap\EHF

2 of 14

Yes

Hsap\ELF5

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (26)

Rnor\Elf2

7 of 14

Yes

Rnor\Elf1

5 of 14

Yes

Rnor\Elf4

5 of 14

Yes

Rnor\Ehf

2 of 14

Yes

Rnor\Elf3

2 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (27)

Mmus\Elf2

6 of 14

Yes

Mmus\Elf4

5 of 14

Yes

Mmus\Elf1

4 of 14

Yes

Mmus\Ehf

2 of 14

Yes

Mmus\Elf3

2 of 14

Yes

Mmus\Elf5

2 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (15)

Xtro\saxo2

5 of 13

Yes

Xtro\elf2

4 of 13

Xtro\elf1

3 of 13

Yes

Xtro\ehf

1 of 13

Yes

Xtro\elf5

1 of 13

Yes

Xtro\elk4

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (27)

Drer\elf2a

6 of 14

Yes

Drer\elf2b

6 of 14

Yes

Drer\elf1

5 of 14

Yes

Drer\ehf

2 of 14

Yes

Drer\etv7

2 of 14

Drer\spdef

2 of 14

Drer\elf3

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (11)

Cele\elf-1

6 of 14

Yes

Cele\ets-7

2 of 14

Yes

Cele\F19F10.1

2 of 14

Yes

Cele\ast-1

1 of 14

Cele\C52B9.19

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\lin-1

1 of 14

Yes

Cele\svh-5

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (8)

Agam\AgaP_AGAP007327

7 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Eip74EF. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (8)

Ets98B

4 of 13

aop

3 of 13

Ets21C

3 of 13

Ets96B

3 of 13

Ets97D

3 of 13

pnt

3 of 13

Ets65A

2 of 13

edl

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Eip74EF^JF02515

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 6

modeled by TER94^R152H.UAS.R

(Chalmers et al., 2023)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ELF2; E74 like ETS transcription factor 2

7 of 14

619798

ELF4; E74 like ETS transcription factor 4

5 of 14

300775

AUTOINFLAMMATORY SYNDROME, FAMILIAL, X-LINKED, BEHCET-LIKE 2; AIFBL2

ELF1; E74 like ETS transcription factor 1

4 of 14

189973

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

Eip74EF - bel

anti tag coimmunoprecipitation, primer specific pcr

(Ihry et al., 2012)

RNA-RNA

Physical Interaction

Assay

References

Eip74EF - mir-34

luminiscence technology, necessary binding region

(Lai et al., 2016, Liu et al., 2012, Burgler and Macdonald, 2005)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Kim et al., 2010)

suppressible

(Liu et al., 2012)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0000567

MIST (protein-protein) - An integrated Molecular Interaction Database

39962

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Apoptosis - fly

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012)

gene_with_start_codon_CUG

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-45

Cytogenetic map

74D4-74E2

Sequence location

3L:17,558,672..17,619,325 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

74D4-74E2

Limits computationally determined from genome sequence between P{lacW}l(3)j11B2^j11B2 and P{PZ}W⁰⁵⁰¹⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

74F1-75A1

(determined by in situ hybridisation)

(Spradling et al., 1999)

74F-74F

(Gibson et al., 1999)

74E-74F

(determined by in situ hybridisation)

(Drosopoulou et al., 1997, Burtis et al., 1990, Janknecht et al., 1989)

74F-74F

(determined by in situ hybridisation)

(Segarra et al., 1996)

74F1-74A1

(BDGP Project Members, 1994-1999)

74F-75A

(determined by in situ hybridisation)

(Cooley et al., 1988)

Experimentally Determined Recombination Data

Location

3-45

(FlyBase, 2016)

3-41.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (56)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Korea

12940

P{VT032333-GAL4}attP2

12941

P{VT032343-GAL4}attP2

13285

P{VT032352-GAL4}attP2

13484

P{VT032349-GAL4}attP2

13763

P{VT032336-GAL4}attP2

14870

P{VT032337-GAL4}attP2

11319

6273R-1

6273R-3

6285-1R-1

v105301

v45900

w¹¹¹⁸ P{GD1433}v45900

More stocks available...

Genomic Clones (37)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (41)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI19001

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0000567

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0000567

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Identified as a candidate gene for hypoxia-specific selection (via an experimental evolution paradigm) that is also differentially expressed between control and hypoxia-adapted larvae.

(Jha et al., 2016)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Eip74EF appears to regulate the timing of hormone-induced cell responses.

(Lee et al., 2002)

Eip74EF is required for salivary gland, autophagic, cell death.

(Lee and Baehrecke, 2001)

Eip74EF isoforms can positively regulate one another. Ectopic expression of Eip74EF can partially repress Hr46 and Eip78C and efficiently repress Eip4F and the late L71 genes. Eip74EFA is both necessary and sufficient for Eip78CB induction, supporting a key role for Eip74EFA in Eip78CB expression. Ectopic Eip74EFA is sufficient to induce only low levels of transcription in some later genes, even in an Eip74EFB mutant background. The ecdysone-triggered isoform switch from an Eip74EFB repressor to an Eip74EFA inducer is necessary, but not sufficient, for the proper timing of late gene induction at puparium formation.

(Fletcher et al., 1997)

Eip74EF is one of a class of genes with TATA-less promoters that have a subset of the conserved DPE sequence.

(Burke and Kadonaga, 1996)

Reduced dosage of the early puff genes Eip74EF and Eip75B reduces the size of the late puffs and increasing the dosage increases the sizes.

(Russell et al., 1996)

Analysis of morphological and molecular phenotypes of double mutants between alleles of br and Eip74EF reveals that br and Eip74EF share functions in puparium formation, pupation and early gene induction.

(Fletcher and Thummel, 1995)

The br and Eip74EF transcription factors may directly interact to regulate the expression of salivary gland glue and late genes.

(Fletcher and Thummel, 1995)

Loss of function mutations of Eip74EF have been used to study the the roles of E74A and E74B proteins during metamorphosis and their roles in salivary gland polytene chromosome puffing hierarchy.

(Fletcher et al., 1995)

Four strong Eip74EF binding sites lie within the shared regulatory sequences of Eig71Ef and Eig71Eg. Results from Ecol\lacZ reporter gene constructs demonstrate these sites are required for the proper timing of Eig71Eg induction.

(Urness and Thummel, 1995)

Comparison of the nucleotide sequence of the 5' leader sequences from Eip74EF, Dpse\Eip74EF and Dvir\Eip74EF has identified 3 exons that specify the leader sequence and identification and characterisation of elements important in controlling expression.

(Jones et al., 1994)

Eip74EF is one of the sites of chromosomal binding of Trl protein.

(Tsukiyama et al., 1994)

Ecdysteroid-regulated gene.

(Andres et al., 1993)

DNA transfections into Drosophila culture cells have been used to define regions of the Eip74EF mRNA required for proper translation initiation. Translation of the Eip74EF protein uses at least three initiator codons: two minor codons (AUG and CUG) and one major CUG codon. Features such as RNA secondary and tertiary structures may play a role in initiator codon selection.

(Boyd and Thummel, 1993)

The function of br is required for the complete ecdysone induction of early mRNAs from Eip74EF, Eip75B and the broad complex itself.

(Karim et al., 1993)

The sequential-temporal expression of steroid hormone-responsive genes in imaginal discs may be important in organizing cellular mechanisms involved in morphogenesis of the epithelium.

(Natzle, 1993)

The developmentally regulated peak of arginine kinase at pupal stage P3 is affected by altering the copy number of Eip75B and BRC, but not Eip74EF.

(James and Collier, 1992)

An ecdysone-inducible gene associated with the early puff at 74E-F.

(Lindsley and Zimm, 1992)

Periodic expression of the gene follows, by approximately one hour, pulses of ecdysone occurring during development; two periods of expression occur independently of ecdysone pulses, one at the end of embryogenesis and one at the end of pupal development. Return to base line levels of expression requires protein synthesis and presumably results from repression by ecdysone-induced gene products; in situ hybridisation to larvae detects transcription of E74 in most tissues, both imaginal and strictly larval.

(Lindsley and Zimm, 1992)

Comparison of Dpse\Eip74EF, Dvir\Eip74EF and Eip74EF shows that several characteristics of the gene have been conserved between the species, including ecdysone inducibility, localisation to ecdysone-induced polytene chromosome puffs, and gene size.

(Jones et al., 1991)

Splicing of the Eip74EF E74A transcript occurs before polyadenylation.

(le Maire and Thummel, 1990)

Relationship to Other Genes

Source for database merge of

Source for merge of: Eip74EF CG6273

(FlyBase Genome Annotators, 2002-2003)

Additional comments

Annotations CG6273 and CG6285 merged as CG32180 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (33)

Reported As

Symbol Synonym

3.3

(Gibson et al., 1999)

74EF

(Tsurumi et al., 2013)

CG32180

(Meadows, 2014.12.12, Baltzer et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009, Lecci et al., 2008)

CG6273

(Boutros et al., 2004, Boutros et al., 2004)

CG6285

(Kanao and Miyachi, 2006)

DmE74

(Lin et al., 2023)

E-74

(Lopez, 1995)

E74

(Umargamwala et al., 2024, Krämer et al., 2023, Carlson et al., 2022, Fu et al., 2022, Li et al., 2022, Pascual-Garcia et al., 2022, Yue et al., 2022, Finger et al., 2021, Tilly et al., 2021, Gao et al., 2020, Song and Zhou, 2020, Jeong et al., 2019, Swevers, 2019, Yoshinari et al., 2019, Ignesti et al., 2018, Lee et al., 2018, Liu et al., 2018, Ables and Drummond-Barbosa, 2017, Yun et al., 2017, Sengupta et al., 2016, Wang et al., 2016, Zheng et al., 2016, Lengil et al., 2015, Xie et al., 2015, Bernardo et al., 2014, Claudius et al., 2014, Kim et al., 2014, Liu et al., 2014, Deng and Kerppola, 2013, Hill et al., 2013, Hyun, 2013, Chauhan et al., 2012, Jin et al., 2012, Liu et al., 2012, Ou et al., 2011, Dialynas et al., 2010, Fraichard et al., 2010, Francis et al., 2010, Rendina et al., 2010, Ritter and Beckstead, 2010, Bernardo et al., 2009, Kozlova et al., 2009, Chen et al., 2008, Costantino et al., 2008, Lecci et al., 2008, Wang et al., 2008, Bashirullah et al., 2007, Huang et al., 2007, Martin et al., 2007, McBrayer et al., 2007, Varghese and Cohen, 2007, Basso et al., 2006, Kanao and Miyachi, 2006, King-Jones et al., 2005, Kon et al., 2005, Lim et al., 2004, McCall, 2004, Westwood et al., 2004, Kumar et al., 2003, Li and White, 2003, Yamazaki et al., 2003, Lee et al., 2002, Pokholkova and Zhimulev, 2002, Thummel and Chory, 2002, Gu et al., 2001, Lee et al., 2001, Thummel, 2001, Venkatesh et al., 2001, Baehrecke, 2000, Hsu and Schulz, 2000, Kas et al., 2000, Kozlova and Thummel, 2000, Lafont, 2000, Lee and Baehrecke, 2000, Lee et al., 2000, Lee et al., 2000, Lee et al., 2000, Pecasse et al., 2000, Reid et al., 2000, Baehrecke and Lee, 1999, Buszczak et al., 1999, Lam et al., 1999, Oettgen et al., 1999, Pastorcic and Das, 1999, Reid et al., 1999, Thummel et al., 1999, Baehrecke and Lee, 1998, Buszczak et al., 1998, D'Avino and Thummel, 1998, Hall and Thummel, 1998, Halsell and Kiehart, 1998, Mo et al., 1998, Reid and Thummel, 1998, Bender et al., 1997, Dhulipal, 1997, Drosopoulou et al., 1997, Dubrovsky and Berger, 1997, Fletcher et al., 1997, Lam et al., 1997, Wilkins and Lis, 1997, Baehrecke, 1996, Bayer et al., 1996, Burke and Kadonaga, 1996, Lehmann, 1996, Segarra et al., 1996, Bender, 1995, Fletcher and Thummel, 1995, Fletcher et al., 1995, Granok et al., 1995, Huet et al., 1995, Andrew and Scott, 1994, Atkinson, 1994, Jindra, 1994, Tsukiyama et al., 1994, Fristrom and Fristrom, 1993, Natzle, 1993, Soeller et al., 1993, Truman et al., 1993, Andres and Thummel, 1992, Arkhipova and Ilyin, 1992, Chen et al., 1992, Oro et al., 1992, Read and Manley, 1992, Thummel, 1992, Arkhipova and Ilyin, 1991, Jarrell and Meselson, 1991, Karim and Thummel, 1991, Ashburner, 1990, Thummel, 1990, Thummel et al., 1990, Urness and Thummel, 1990, Thummel, 1989)

E74-B

(Karlin and Burge, 1996)

E74A

(Ostalé et al., 2024, Chebbo et al., 2021, Xu et al., 2020, Kang et al., 2017, Zheng et al., 2017, Valzania et al., 2016, Nicolson et al., 2015, Yan et al., 2015, Gündner et al., 2014, Ihry and Bashirullah, 2014, Mulakkal et al., 2014, Neuman et al., 2014, Chauhan et al., 2013, Denton et al., 2013, Morozov and Ioshikhes, 2013, Anhezini et al., 2012, Ihry et al., 2012, Akagi and Ueda, 2011, Ou et al., 2011, Walker et al., 2011, Capelson et al., 2010, Ruaud et al., 2010, van der Knaap et al., 2010, Wang et al., 2010, Chen et al., 2008, Beckstead et al., 2007, Cao et al., 2007, Huang et al., 2007, McBrayer et al., 2007, Thummel, 2007, Yoshikane et al., 2007, Caldwell et al., 2006, Ciurciu et al., 2006, Kraft et al., 2006, Sun and Song, 2006, Wegman et al., 2006, Beckstead et al., 2005, Caldwell et al., 2005, Kilpatrick et al., 2005, Kumar and Cakouros, 2004, Martin and Baehrecke, 2004, Bashirullah et al., 2003, Daish et al., 2003, Basso et al., 2002, Bialecki et al., 2002, Lee et al., 2002, Lee et al., 2002, Lee et al., 2002, Lehmann et al., 2002, Pile et al., 2002, Beckstead et al., 2001, Biyasheva et al., 2001, Gibson and Schubiger, 2001, Lee and Baehrecke, 2001, Lee et al., 2001, Simon et al., 2001, Thummel, 2001, Hock et al., 2000, Jiang et al., 2000, Kutach and Kadonaga, 2000, Lafont, 2000, Li and Bender, 2000, Broadus et al., 1999, Burden and Osheroff, 1999, Richards et al., 1999, Burke et al., 1998, Buszczak and Segraves, 1998, Fisk and Thummel, 1998, Lam et al., 1997, Thummel, 1997, Venkatesh and Hasan, 1997, White et al., 1997, Huet et al., 1996, Meister and Richards, 1996, Baehrecke and Thummel, 1995, D'Avino et al., 1995, Henrich and Brown, 1995, Urness and Thummel, 1995, Jones et al., 1994, Woodard et al., 1994, Andres et al., 1993, Bornschein et al., 1993, Boyd and Thummel, 1993, Karim et al., 1993, Riddiford, 1993, Wasylyk et al., 1993, Chen et al., 1992, Karim and Thummel, 1992, Boyd et al., 1991, Burtis et al., 1990, le Maire and Thummel, 1990)

E74A/B

(Badenhorst et al., 2005)

E74B

(Li et al., 2023, Mirzoyan et al., 2023, Dong et al., 2022, Heredia et al., 2021, Ma et al., 2020, Valenza et al., 2018, Hindle et al., 2017, Jaszczak et al., 2015, Yan et al., 2015, Charroux and Royet, 2014, Mirth et al., 2014, Neuman et al., 2014, Ihry et al., 2012, Lin et al., 2011, Ou et al., 2011, Storelli et al., 2011, Parry et al., 2010, Slaidina et al., 2009, Walkiewicz and Stern, 2009, Chen et al., 2008, Juven-Gershon et al., 2008, Layalle et al., 2008, Beckstead et al., 2007, McBrayer et al., 2007, Caldwell et al., 2006, Sun and Song, 2006, Caldwell et al., 2005, Kilpatrick et al., 2005, Bashirullah et al., 2003, Basso et al., 2002, Bialecki et al., 2002, Lee et al., 2002, Beckstead et al., 2001, Hock et al., 2000, Kutach and Kadonaga, 2000, Lafont, 2000, Broadus et al., 1999, Lam et al., 1999, Richards et al., 1999, Schubiger and Truman, 1999, Burke et al., 1998, Buszczak and Segraves, 1998, Fisk and Thummel, 1998, Lam et al., 1997, Venkatesh and Hasan, 1997, Huet et al., 1996, Meister and Richards, 1996, D'Avino et al., 1995, Henrich and Brown, 1995, Andres et al., 1993, Riddiford, 1993, Karim and Thummel, 1992, Burtis et al., 1990)

ECIP

(Bondinas et al., 2002, Bondinas et al., 2001)

Eip74

(Shlyueva et al., 2014, Slattery et al., 2014)

Eip74 A

(Ciurciu et al., 2006)

Eip74A

(Beckstead et al., 2005)

Eip74E

(Sonnenfeld et al., 2005)

Eip74EF

(McPherson et al., 2024, Keith, 2023, Sebastian et al., 2022, Wang et al., 2022, Chebbo et al., 2021, DeAngelis et al., 2021, Cho et al., 2020, Khoroshko et al., 2020, Kinser and Pincus, 2020, Theis et al., 2020, Zraly et al., 2020, Meltzer et al., 2019, Geronikolou et al., 2018, Yao et al., 2017, Jha et al., 2016, Kwon et al., 2016, Lai et al., 2016, Xiong et al., 2016, Hsu et al., 2015, Kern et al., 2015, Lengil et al., 2015, Xie et al., 2015, Ashwal-Fluss et al., 2014, Boyle et al., 2014, Rogers et al., 2014, Toshima et al., 2014, Chauhan et al., 2013, Li and Gilmour, 2013, Song et al., 2013, Webber et al., 2013, Bodai et al., 2012, Chauhan et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Liu et al., 2012, Lv et al., 2012, Rodriguez et al., 2012, Spokony and White, 2012.5.22, Stern et al., 2012, Gancz et al., 2011, Turner et al., 2011, Walker et al., 2011, Kim et al., 2010, Pauli et al., 2010, Sawatsubashi et al., 2010, Baltzer et al., 2009, Schaaf et al., 2009, Christensen et al., 2008.4.15, Christensen et al., 2008.4.15, Christensen et al., 2008.4.15, Gilchrist et al., 2008, Hoopfer et al., 2008, Lecci et al., 2008, Weake et al., 2008, Curtis et al., 2007, Huang et al., 2007, Martin et al., 2007, Zeitouni et al., 2007, Burgler and Macdonald, 2005, Copley, 2005, Hoskins et al., 2005)

e74

(Bergman and Kreitman, 2001, Karlin et al., 1990)

e74B

(Koyama et al., 2014)

e74b

(Kannangara et al., 2020)

eip74EF

(Koyama et al., 2014)

eip74ef

(Krasnov et al., 2023, Foglietti et al., 2006)

l(3)24

l(3)neo24

mE74

(Jones et al., 1991)

Name Synonyms

Ecdysone-induced prot 74EF

(Carpenter et al., 2009)

Ecdysone-induced protein 74EF

(Jha et al., 2016, Toshima et al., 2014, Zeitouni et al., 2007)

Ecdysone-induced-protein 74EF

(Huang et al., 2007)

ecdysone E74

(Chetverina et al., 2021)

ecdysone induced protein 74EF

(Kim et al., 2010)

ecdysone inducible protein

(Bondinas et al., 2001)

ecdysone-induced protein 74EF

(Mirth et al., 2014)

Secondary FlyBase IDs

FBgn0011459
FBgn0026806
FBgn0036739
FBgn0052180

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 93 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39962

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P11536

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P11536

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0000567

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39962/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0000567

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0000567

FlyMine - An integrated database for Drosophila genomics

FBgn0000567

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG32180

MARRVEL_MODEL - MARRVEL (model organism gene)

39962

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0000567

DroID - A comprehensive database of gene and protein interactions.

FBgn0000567

DRSC - Results frm RNAi screens

FBgn0000567

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0000567

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0000567

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG32180

Flygut - An atlas of the Drosophila adult midgut

FBgn0000567

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0000567

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC031968

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Apoptosis - fly

MIST (protein-protein) - An integrated Molecular Interaction Database

39962

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (407)

The following external sites may use different assemblies or annotations than FlyBase.

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