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Gene Dmel\Eip74EF

General Information
Symbol	Dmel\Eip74EF	Species	D. melanogaster
Name	Ecdysone-induced protein 74EF	Annotation symbol	CG32180
Feature type	protein_coding_gene	FlyBase ID	FBgn0000567
Created / Updated	2005-01-10/2005-01-10
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	74D4-74E2	Sequence location	3L:17,553,258..17,612,407 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Ecdysone-induced protein 74EF is referred to in FlyBase by the symbol Eip74EF (CG32180, FBgn0000567). It has the cytological map location 74D4-74E2. Its sequence location is 3L:17553258..17612407. Its molecular function is described as: transcription factor activity; specific RNA polymerase II transcription factor activity; sequence-specific DNA binding. It is involved in the biological processes: salivary gland cell autophagic cell death; autophagy; cell death; regulation of transcription; regulation of development, heterochronic; regulation of transcription, DNA-dependent; cellular process; oogenesis. 37 alleles are reported. The phenotypes of these alleles are annotated with: larva; cuticle; adult epidermis; salivary gland; air bubble; anterior pupal spiracle; procephalic segment. It has 4 annotated transcripts and 4 annotated polypeptides.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	E74: Ecdysone-inducible gene encoded in region 74 (W.A. Segraves) Encodes an ecdysone-inducible gene associated with the early puff at 74E-F. Periodic expression of the gene follows, by approximately one hour, pulses of ecdysone occurring during development; two periods of expression occur independently of ecdysone pulses, one at the end of embryogenesis and one at the end of pupal development. Return to base line levels of expression requires protein synthesis and presumably results from repression by ecdysone-induced gene products; in situ hybridization to larvae detects transcription of E74 in most tissues, both imaginal and strictly larval.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 74D4-74E2   Limits computationally determined from genome sequence between P{lacW}l(3)j11B2j11B2 and P{PZ}W05014
Experimentally Determined Cytological Location
Cytogenetic map Notes References 74F1-75A1 (determined by in situ hybridisation)   (Spradling et al., 1999) 74F-74F   (Gibson et al., 1999) 74E-74F (determined by in situ hybridisation)   (Drosopoulou et al., 1997) 74F-74F (determined by in situ hybridisation)   (Segarra et al., 1996) 74F1-74A1   (BDGP Project Members, 1994-1999) 74E-74F (determined by in situ hybridisation)   (Burtis et al., 1990) 74E-74F (determined by in situ hybridisation)   (Janknecht et al., 1989) 74F-75A (determined by in situ hybridisation)   (Cooley et al., 1988)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: Jon74E- CycT- Eip74EF- CG6259- CG7510+ (Burtis et al., 1990) Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Eip74EF for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Eip74EF-RA FBtr0075202  NM_168740   5994   829 Eip74EF-RB FBtr0075204  NM_168741   5059   883 Eip74EF-RC FBtr0075203  NM_168739   5994   868 Eip74EF-RD FBtr0100411  NM_001014590   4753   883
Additional Transcript Data & Comments
Reported size (kB)	6.0 (northern blot) (Chen et al., 1992) 6.0, 5.1, 4.8 (northern blot) (Burtis et al., 1990)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Eip74EF-PA   FBpp0074965   87.1   829   6.53     NP_730287   AAF49323 Eip74EF-PB   FBpp0074967   94.8   883   6.90     NP_730288   AAF49324 Eip74EF-PC   FBpp0074966   91.3   868   6.48     NP_730286   AAN11697 Eip74EF-PD   FBpp0099825   94.8   883   6.90     NP_001014590   AAX52740
Additional Polypeptide Data & Comments
Reported size (kD)	883, 829 (aa); 94.7, 87.1 (kD predicted) (Burtis et al., 1990) 883 (aa) (Lautenberger et al., 1992)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0000567
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Ets (IPR000418) Winged helix repressor DNA-binding (IPR011991) TRANSFAC - Eukaryotic transcription factors, their genomic binding sites, and DNA-binding profiles • R01862 R01863 R01864 R01865 R01866 R01867 R01868 R01869 R02386 R02387 R02388 T00208 T00210
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE003523 AAF49323   AE003523 AAF49324   AE003523 AAN11697   AI257604   AI258329   AI258330   AI532175   AI533771   AI541952   AL008814   AQ034100   AW943973   AW944316   AY060446 AAL25485   BF503334   BI483176   BI611587   BT003745   BT029430   CO264520   CO278632   CO280885   CO285872   CO292571   CO302879   CO313874   CO318057   CO321886   CO330450   CZ467470   CZ471672   CZ471715   CZ471883   CZ471948   CZ472767   CZ473568   CZ474048   CZ475029   CZ475493   CZ475509   CZ475515   CZ476278   CZ476498   CZ477540   CZ477736   CZ478831   CZ480169   CZ482180   CZ482181   CZ485400   CZ485401   CZ489498   CZ489709   DN153951   EC060973   EC063014   EC067469   EC068154   EC071297   EC072684   EC074471   EC087115   EC194431   EC201149   EC202660   EC202779   EC209802   EC214368   EC226279   EC229404   EC233902   EC234055   EC234911   EC238738   EC241804   EC260383   EL884302   EL884303   EL884431   EL884432   EL884667   M37082 AAA28493   M37083 AAA28494   X15087 CAA33195
UniProtKB/Swiss-Prot	P11536 P20105
UniProtKB/TrEMBL	Q95SX3
Maps to
Does NOT map to
Identified with	LP01487 SD03570 (BDGP Project Members, 2000-) LP01488 Dm4321 (Spradling et al., 1999) D1239 (BDGP Project Members, 1994-1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References protein binding site Eip74EF-protein_bind-1 3L:17,612,519..17,612,536 bound_moiety=Trl-XP evidence=experimental (Thummel, 1989, Bergman et al., 2004) protein binding site Eip74EF-protein_bind-10 3L:17,612,597..17,612,622 bound_moiety=Trl-XP evidence=experimental (Bergman et al., 2004, Thummel, 1989) protein binding site Eip74EF-protein_bind-11 3L:17,580,748..17,580,767 bound_moiety=Eip74EF-XP evidence=experimental (Urness and Thummel, 1990, Bergman et al., 2004) protein binding site Eip74EF-protein_bind-2 3L:17,580,638..17,580,660 bound_moiety=Eip74EF-XP evidence=experimental (Bergman et al., 2004, Urness and Thummel, 1990) protein binding site Eip74EF-protein_bind-3 3L:17,580,841..17,580,861 bound_moiety=Eip74EF-XP evidence=experimental (Urness and Thummel, 1990, Bergman et al., 2004) protein binding site Eip74EF-protein_bind-4 3L:17,612,506..17,612,523 bound_moiety=z-XP evidence=experimental (Thummel, 1989, Bergman et al., 2004) protein binding site Eip74EF-protein_bind-5 3L:17,612,455..17,612,470 bound_moiety=z-XP evidence=experimental (Thummel, 1989, Bergman et al., 2004) protein binding site Eip74EF-protein_bind-6 3L:17,612,569..17,612,584 bound_moiety=Trl-XP evidence=experimental (Thummel, 1989, Bergman et al., 2004) protein binding site Eip74EF-protein_bind-7 3L:17,612,503..17,612,513 bound_moiety=Trl-XP evidence=experimental (Bergman et al., 2004, Thummel, 1989) protein binding site Eip74EF-protein_bind-8 3L:17,612,455..17,612,472 bound_moiety=Trl-XP evidence=experimental (Bergman et al., 2004, Thummel, 1989) protein binding site Eip74EF-protein_bind-9 3L:17,612,549..17,612,564 bound_moiety=Trl-XP evidence=experimental (Thummel, 1989, Bergman et al., 2004)
External Data
Linkouts
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  prepupal stage   (Bashirullah et al., 2003)  pupal stage | 6-10 h   (Bashirullah et al., 2003)  prepupal stage   (Bashirullah et al., 2003)  pupal stage | 0-6 h   (Bashirullah et al., 2003)  pupal stage | 10-12 h   (Bashirullah et al., 2003)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term	nucleus (Burtis et al., 1990)
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI258329 AI532175
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000567 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0000567 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0000567
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Eip74EF2 lethal | recessive Eip74EFDL-1 Eip74EFneo24 lethal | larval stage Eip74EFDL-1 lethal | pharate adult stage Eip74EFneo24 lethal | pharate adult stage | recessive Eip74EF1 Other Phenotypes Allele cell death decrease Eip74EFneo24 Phenotype manifest in Allele larva Eip74EF2 cuticle Eip74EF2 air bubble Eip74EFDL-1 anterior pupal spiracle Eip74EFDL-1 procephalic segment Eip74EFDL-1 larval midgut & prepupa (with Df(3L)81k19) Eip74EFDL-1 Eip74EFneo24 adult epidermis Eip74EFneo24 larval salivary gland & embryo Eip74EFneo24 salivary gland Eip74EFneo24
Classical Alleles ( 33 )
For All Classical Alleles Show
Allele of Eip74EF Class Mutagen Stocks Known lesion Eip74EF1 X ray 0 Yes Eip74EF2 ethyl methanesulfonate 0 -- Eip74EFBG01805 P-element activity 1 -- Eip74EFBG02525 P-element activity 1 -- Eip74EFDG12412 P-element activity 1 -- Eip74EFDL-1 loss of function ethyl methanesulfonate 2 Yes Eip74EFPA-9 0 -- Eip74EFc01811 piggyBac transposase 1 -- Eip74EFd00570 P-element activity 1 -- Eip74EFd00633 P-element activity 1 -- Eip74EFd00907 P-element activity 1 -- Eip74EFd00990 P-element activity 1 -- Eip74EFd02346 P-element activity 1 -- Eip74EFd03802 P-element activity 0 -- Eip74EFd04647 P-element activity 1 -- Eip74EFd06283 P-element activity 1 -- Eip74EFd07032 P-element activity 1 -- Eip74EFd07054 P-element activity 1 -- Eip74EFd07067 P-element activity 0 -- Eip74EFd08528 P-element activity 1 -- Eip74EFd08971 P-element activity 1 -- Eip74EFd11079 P-element activity 0 -- Eip74EFd11456 P-element activity 1 -- Eip74EFe01002 piggyBac transposase 1 -- Eip74EFe02347 piggyBac transposase 1 -- Eip74EFe04421 piggyBac transposase 0 -- Eip74EFe04422 piggyBac transposase 1 -- Eip74EFf02844 piggyBac transposase 1 -- Eip74EFf06771 piggyBac transposase 1 -- Eip74EFf06965 piggyBac transposase 1 -- Eip74EFneo24 amorph, loss of function P-element activity 1 Yes Eip74EFunspecified 0 -- Eip74EFv4 2 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Eip74EF Class Mutagen Stocks Known lesion Eip74EFA.dsRNA.cKa in vitro construct | RNAi 0 Yes Eip74EFA.hs in vitro construct | regulatory fusion 0 Yes Eip74EFB.dsRNA.cKa in vitro construct | RNAi 0 Yes Eip74EFB.hs in vitro construct | regulatory fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(3L)81k19
Useful duplication	Dp(3;3)st+g18
Disrupted in	Df(3L)81k19 (Halsell and Kiehart, 1998, Spradling et al., 1999, Lee and Baehrecke, 2001, James and Collier, 1992, BDGP Project Members, 1994-1999, Russell et al., 1996) Df(3L)BSC415 (Christensen et al., 2008.4.15) Df(3L)BSC432 (Christensen et al., 2008.4.15) Df(3L)BSC444 (Christensen et al., 2008.4.15) Df(3L)BSC8 (Deal and Cook, 2001.12.6) Df(3L)Exel9005 (Ryder, 2004.4.26)