E74, E74A, E74B, Eip74, ECIP
ETS domain transcription factor that encodes two isoforms that control the timing of gene induction during molting
Please see the JBrowse view of Dmel\Eip74EF for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Unconventional translation start (CUG) postulated; FBrf0051390, FBrf0064374.
Gene model reviewed during 5.46
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
6.0 (northern blot)
6.0, 5.1, 4.8 (northern blot)
The group(s) of polypeptides indicated below share identical sequence to each other.
883 (aa)
883, 829 (aa); 94.7, 87.1 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Eip74EF using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: from -12 hr APF
Comment: maternally deposited
Comment: 12-24 hr AEL
Comment: 4 hr before puparium formation
Comment: 0-6 hr APF
Comment: 10-12 hr APF
Comment: 12 hr APF
Comment: Assay specific to Eip74EF transcripts Eip74EF-RA (E74A) and Eip74EF-RB (E74B).
Comment: Assay specific to Eip74EF transcript Eip74EF-RA (E74A) and Eip74EF-RB (E74B). Only Eip74EF-RA is expressed.
Comment: Assay specific to Eip74EF transcript Eip74EF-RA (E74A).
Expression increases after the late larval (-4 hr APF) and prepupal (+10 hr APF) ecdysone pulses.
Eip74EF transcript Eip74EF-RA is expressed in pupa and adult males. Transcript Eip74EF-RB is absent from adult males.
Expression of Eip74EF coincides with a pulse of ecdysone shortly before puparium formation, and a second wave at 10 hours after pupation.
Eip74EF transcript is strongly expressed in salivary glands at the late larval and late prepupal ecdysone pulses.
RNA blots were carried out on RNA extracted from staged larval and prepupal salivary glands. Eip74EF transcripts are induced in apparent response to the late-larval and prepupal ecdysone pulses paralleling the puffing response at 74EF. This response is enhanced in response to ectopic ftz-f1 expression.
Peaks of Eip74EF expression are seen in late embryos, late third instar larvae, and mid pupae.
Comment: Adults younger than 12h. Assay specific to Eip74EF isoform Eip74EF-PA (E74A).
Comment: Assay specific to Eip74EF isoform Eip74EF-PA (E74A).
Eip74EF protein isoform Eip74EF-PA is strongly expressed in the head of adults younger than 12h. Expression decreases rapidly and from day 1 onwards, weak expression is observed.
JBrowse - Visual display of RNA-Seq signals
View Dmel\Eip74EF in JBrowse3-45
3-41.1
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Identified as a candidate gene for hypoxia-specific selection (via an experimental evolution paradigm) that is also differentially expressed between control and hypoxia-adapted larvae.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Eip74EF appears to regulate the timing of hormone-induced cell responses.
Eip74EF is required for salivary gland, autophagic, cell death.
Eip74EF isoforms can positively regulate one another. Ectopic expression of Eip74EF can partially repress Hr46 and Eip78C and efficiently repress Eip4F and the late L71 genes. Eip74EFA is both necessary and sufficient for Eip78CB induction, supporting a key role for Eip74EFA in Eip78CB expression. Ectopic Eip74EFA is sufficient to induce only low levels of transcription in some later genes, even in an Eip74EFB mutant background. The ecdysone-triggered isoform switch from an Eip74EFB repressor to an Eip74EFA inducer is necessary, but not sufficient, for the proper timing of late gene induction at puparium formation.
Eip74EF is one of a class of genes with TATA-less promoters that have a subset of the conserved DPE sequence.
Loss of function mutations of Eip74EF have been used to study the the roles of E74A and E74B proteins during metamorphosis and their roles in salivary gland polytene chromosome puffing hierarchy.
Comparison of the nucleotide sequence of the 5' leader sequences from Eip74EF, Dpse\Eip74EF and Dvir\Eip74EF has identified 3 exons that specify the leader sequence and identification and characterisation of elements important in controlling expression.
Ecdysteroid-regulated gene.
DNA transfections into Drosophila culture cells have been used to define regions of the Eip74EF mRNA required for proper translation initiation. Translation of the Eip74EF protein uses at least three initiator codons: two minor codons (AUG and CUG) and one major CUG codon. Features such as RNA secondary and tertiary structures may play a role in initiator codon selection.
The sequential-temporal expression of steroid hormone-responsive genes in imaginal discs may be important in organizing cellular mechanisms involved in morphogenesis of the epithelium.
An ecdysone-inducible gene associated with the early puff at 74E-F.
Periodic expression of the gene follows, by approximately one hour, pulses of ecdysone occurring during development; two periods of expression occur independently of ecdysone pulses, one at the end of embryogenesis and one at the end of pupal development. Return to base line levels of expression requires protein synthesis and presumably results from repression by ecdysone-induced gene products; in situ hybridisation to larvae detects transcription of E74 in most tissues, both imaginal and strictly larval.
Comparison of Dpse\Eip74EF, Dvir\Eip74EF and Eip74EF shows that several characteristics of the gene have been conserved between the species, including ecdysone inducibility, localisation to ecdysone-induced polytene chromosome puffs, and gene size.
Splicing of the Eip74EF E74A transcript occurs before polyadenylation.
Source for merge of: Eip74EF CG6273
Annotations CG6273 and CG6285 merged as CG32180 in release 3 of the genome annotation.