Identified as a candidate gene for hypoxia-specific selection (via an experimental evolution paradigm) that is also differentially expressed between control and hypoxia-adapted larvae.

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

Eip74EF appears to regulate the timing of hormone-induced cell responses.

Eip74EF is required for salivary gland, autophagic, cell death.

Eip74EF isoforms can positively regulate one another. Ectopic expression of Eip74EF can partially repress Hr46 and Eip78C and efficiently repress Eip4F and the late L71 genes. Eip74EFA is both necessary and sufficient for Eip78CB induction, supporting a key role for Eip74EFA in Eip78CB expression. Ectopic Eip74EFA is sufficient to induce only low levels of transcription in some later genes, even in an Eip74EFB mutant background. The ecdysone-triggered isoform switch from an Eip74EFB repressor to an Eip74EFA inducer is necessary, but not sufficient, for the proper timing of late gene induction at puparium formation.

Eip74EF is one of a class of genes with TATA-less promoters that have a subset of the conserved DPE sequence.

Reduced dosage of the early puff genes Eip74EF and Eip75B reduces the size of the late puffs and increasing the dosage increases the sizes.

Analysis of morphological and molecular phenotypes of double mutants between alleles of br and Eip74EF reveals that br and Eip74EF share functions in puparium formation, pupation and early gene induction.

The br and Eip74EF transcription factors may directly interact to regulate the expression of salivary gland glue and late genes.

Loss of function mutations of Eip74EF have been used to study the the roles of E74A and E74B proteins during metamorphosis and their roles in salivary gland polytene chromosome puffing hierarchy.

Four strong Eip74EF binding sites lie within the shared regulatory sequences of Eig71Ef and Eig71Eg. Results from Ecol\lacZ reporter gene constructs demonstrate these sites are required for the proper timing of Eig71Eg induction.

Comparison of the nucleotide sequence of the 5' leader sequences from Eip74EF, Dpse\Eip74EF and Dvir\Eip74EF has identified 3 exons that specify the leader sequence and identification and characterisation of elements important in controlling expression.

Eip74EF is one of the sites of chromosomal binding of Trl protein.

Ecdysteroid-regulated gene.

DNA transfections into Drosophila culture cells have been used to define regions of the Eip74EF mRNA required for proper translation initiation. Translation of the Eip74EF protein uses at least three initiator codons: two minor codons (AUG and CUG) and one major CUG codon. Features such as RNA secondary and tertiary structures may play a role in initiator codon selection.

The function of br is required for the complete ecdysone induction of early mRNAs from Eip74EF, Eip75B and the broad complex itself.

The sequential-temporal expression of steroid hormone-responsive genes in imaginal discs may be important in organizing cellular mechanisms involved in morphogenesis of the epithelium.

The developmentally regulated peak of arginine kinase at pupal stage P3 is affected by altering the copy number of Eip75B and BRC, but not Eip74EF.

An ecdysone-inducible gene associated with the early puff at 74E-F.

Periodic expression of the gene follows, by approximately one hour, pulses of ecdysone occurring during development; two periods of expression occur independently of ecdysone pulses, one at the end of embryogenesis and one at the end of pupal development. Return to base line levels of expression requires protein synthesis and presumably results from repression by ecdysone-induced gene products; in situ hybridisation to larvae detects transcription of E74 in most tissues, both imaginal and strictly larval.

Comparison of Dpse\Eip74EF, Dvir\Eip74EF and Eip74EF shows that several characteristics of the gene have been conserved between the species, including ecdysone inducibility, localisation to ecdysone-induced polytene chromosome puffs, and gene size.

Splicing of the Eip74EF E74A transcript occurs before polyadenylation.