Gene Dmel\Eip75B

75B-75B

(determined by in situ hybridisation)

(Feigl et al., 1989)

75B-75B

(determined by in situ hybridisation)

(Cooley et al., 1988)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\Eip75B for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Low-frequency RNA-Seq exon junction(s) not annotated.

Two sites of polyadenylation have been reported by Eip75B (see GB:X51548, GB:X51549, and GB:15586).

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Eip75B-RA

FBtr0075150

NM_079409

6077

1355

Eip75B-RB

FBtr0075148

NM_168755

4536

1412

Eip75B-RC

FBtr0075149

NM_168756

5769

1199

Eip75B-RD

FBtr0075151

NM_168757

3750

906

Eip75B-RE

FBtr0306813

NM_001259892

5849

1412

Eip75B-RF

FBtr0306814

NM_001259893

8236

1412

Additional Transcript Data & Comments

Reported size (kB)

6, 5.7, 5.2, 4.9 (northern blot)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Eip75B-PA

FBpp0074916

147.2

1355

7.84

NP_524133

AAN11688

Eip75B-PB

FBpp0074914

151.3

1412

9.44

NP_730321

AAF49282

Eip75B-PC

FBpp0074915

128.5

1199

8.37

NP_730322

AAN11687

Eip75B-PD

FBpp0074917

96.4

906

8.30

NP_730323

AAN11689

Eip75B-PE

FBpp0297725

151.3

1412

9.44

NP_001246821

AFH04492

Eip75B-PF

FBpp0297726

151.3

1412

9.44

NP_001246822

AFH04493

Additional Polypeptide Data & Comments

Reported size (kDa)

1394, 1237 (aa); 151, 132 (kD predicted)

Comments

One of a couple of protein products.

(FlyBase, 1992-, FlyBase, 1992-, FlyBase, 1992-)

External Data

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Nuclear hormone receptor, ligand-binding, core (IPR000536)

Zinc finger, nuclear hormone receptor-type (IPR001628)

Steroid hormone receptor (IPR001723)

Nuclear hormone receptor, ligand-binding (IPR008946)

Zinc finger, NHR/GATA-type (IPR013088)

Thyroid hormone receptor (IPR001728)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

(Fisher et al., 2012)

Comment:maternally deposited

embryonic stage 13 -- 16

embryonic/larval midgut

(Fisher et al., 2012)

organism | faint & ubiquitous

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval salivary gland

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

References

nucleus

inferred from direct assay

(The modENCODE Consortium, 2010, Graveley et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval midgut, larval hindgut, larval Malpighian tubules, larval/adult fat body, larval/adult salivary gland, larval trachea, adult ovary, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		71
central nervous system, pupae P8		72
head, virgin 1-day female		11
head, virgin 4-day female		11
head, virgin 20-day female		11
head, mated 1-day female		11
head, mated 4-day female		13
head, mated 20-day female		12
head, mated 1-day male		19
head, mated 4-day male		18
head, mated 20-day male		17
salivary gland, larvae L3 wandering		8
salivary gland, white prepupae		12
digestive system, larvae L3 wandering		15
digestive system, 1-day adult		5
digestive system, 4-day adult		8
digestive system, 20-day adult		9
fat body, larvae L3 wandering		14
fat body, white prepupae		34
fat body, pupae P8		31
carcass, larvae L3 wandering		34
carcass, 1-day adult		8
carcass, 4-day adult		9
carcass, 20-day adult		10
ovary, virgin 4-day female		4
ovary, mated 4-day female		4
testis, mated 4-day male		1
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		(71)
central nervous system, pupae P8		(72)
head, virgin 1-day female		11
head, virgin 4-day female		11
head, virgin 20-day female		11
head, mated 1-day female		11
head, mated 4-day female		13
head, mated 20-day female		12
head, mated 1-day male		19
head, mated 4-day male		18
head, mated 20-day male		17
salivary gland, larvae L3 wandering		8
salivary gland, white prepupae		12
digestive system, larvae L3 wandering		15
digestive system, 1-day adult		5
digestive system, 4-day adult		8
digestive system, 20-day adult		9
fat body, larvae L3 wandering		14
fat body, white prepupae		(34)
fat body, pupae P8		(31)
carcass, larvae L3 wandering		(34)
carcass, 1-day adult		8
carcass, 4-day adult		9
carcass, 20-day adult		10
ovary, virgin 4-day female		4
ovary, mated 4-day female		4
testis, mated 4-day male		1
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		71
central nervous system, pupae P8		72
head, virgin 1-day female		11
head, virgin 4-day female		11
head, virgin 20-day female		11
head, mated 1-day female		11
head, mated 4-day female		13
head, mated 20-day female		12
head, mated 1-day male		19
head, mated 4-day male		18
head, mated 20-day male		17
salivary gland, larvae L3 wandering		8
salivary gland, white prepupae		12
digestive system, larvae L3 wandering		15
digestive system, 1-day adult		5
digestive system, 4-day adult		8
digestive system, 20-day adult		9
fat body, larvae L3 wandering		14
fat body, white prepupae		34
fat body, pupae P8		31
carcass, larvae L3 wandering		34
carcass, 1-day adult		8
carcass, 4-day adult		9
carcass, 20-day adult		10
ovary, virgin 4-day female		4
ovary, mated 4-day female		4
testis, mated 4-day male		1
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		71
central nervous system, pupae P8		72
head, virgin 1-day female		11
head, virgin 4-day female		11
head, virgin 20-day female		11
head, mated 1-day female		11
head, mated 4-day female		13
head, mated 20-day female		12
head, mated 1-day male		19
head, mated 4-day male		18
head, mated 20-day male		17
salivary gland, larvae L3 wandering		8
salivary gland, white prepupae		12
digestive system, larvae L3 wandering		15
digestive system, 1-day adult		5
digestive system, 4-day adult		8
digestive system, 20-day adult		9
fat body, larvae L3 wandering		14
fat body, white prepupae		34
fat body, pupae P8		31
carcass, larvae L3 wandering		34
carcass, 1-day adult		8
carcass, 4-day adult		9
carcass, 20-day adult		10
ovary, virgin 4-day female		4
ovary, mated 4-day female		4
testis, mated 4-day male		1
accessory gland, mated 4-day male		4
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		71
central nervous system, pupae P8		72
head, virgin 1-day female		11
head, virgin 4-day female		11
head, virgin 20-day female		11
head, mated 1-day female		11
head, mated 4-day female		13
head, mated 20-day female		12
head, mated 1-day male		19
head, mated 4-day male		18
head, mated 20-day male		17
salivary gland, larvae L3 wandering		8
salivary gland, white prepupae		12
digestive system, larvae L3 wandering		15
digestive system, 1-day adult		5
digestive system, 4-day adult		8
digestive system, 20-day adult		9
fat body, larvae L3 wandering		14
fat body, white prepupae		34
fat body, pupae P8		31
carcass, larvae L3 wandering		34
carcass, 1-day adult		8
carcass, 4-day adult		9
carcass, 20-day adult		10
ovary, virgin 4-day female		4
ovary, mated 4-day female		4
testis, mated 4-day male		1
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		(71)
central nervous system, pupae P8		(72)
head, virgin 1-day female		11
head, virgin 4-day female		11
head, virgin 20-day female		11
head, mated 1-day female		11
head, mated 4-day female		13
head, mated 20-day female		12
head, mated 1-day male		19
head, mated 4-day male		18
head, mated 20-day male		17
salivary gland, larvae L3 wandering		8
salivary gland, white prepupae		12
digestive system, larvae L3 wandering		15
digestive system, 1-day adult		5
digestive system, 4-day adult		8
digestive system, 20-day adult		9
fat body, larvae L3 wandering		14
fat body, white prepupae		(34)
fat body, pupae P8		31
carcass, larvae L3 wandering		(34)
carcass, 1-day adult		8
carcass, 4-day adult		9
carcass, 20-day adult		10
ovary, virgin 4-day female		4
ovary, mated 4-day female		4
testis, mated 4-day male		1
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		71
central nervous system, pupae P8		72
head, virgin 1-day female		11
head, virgin 4-day female		11
head, virgin 20-day female		11
head, mated 1-day female		11
head, mated 4-day female		13
head, mated 20-day female		12
head, mated 1-day male		19
head, mated 4-day male		18
head, mated 20-day male		17
salivary gland, larvae L3 wandering		8
salivary gland, white prepupae		12
digestive system, larvae L3 wandering		15
digestive system, 1-day adult		5
digestive system, 4-day adult		8
digestive system, 20-day adult		9
fat body, larvae L3 wandering		14
fat body, white prepupae		34
fat body, pupae P8		31
carcass, larvae L3 wandering		34
carcass, 1-day adult		8
carcass, 4-day adult		9
carcass, 20-day adult		10
ovary, virgin 4-day female		4
ovary, mated 4-day female		4
testis, mated 4-day male		1
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		71
central nervous system, pupae P8		72
head, virgin 1-day female		11
head, virgin 4-day female		11
head, virgin 20-day female		11
head, mated 1-day female		11
head, mated 4-day female		13
head, mated 20-day female		12
head, mated 1-day male		19
head, mated 4-day male		18
head, mated 20-day male		17
salivary gland, larvae L3 wandering		8
salivary gland, white prepupae		12
digestive system, larvae L3 wandering		15
digestive system, 1-day adult		5
digestive system, 4-day adult		8
digestive system, 20-day adult		9
fat body, larvae L3 wandering		14
fat body, white prepupae		34
fat body, pupae P8		31
carcass, larvae L3 wandering		34
carcass, 1-day adult		8
carcass, 4-day adult		9
carcass, 20-day adult		10
ovary, virgin 4-day female		4
ovary, mated 4-day female		4
testis, mated 4-day male		1
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed during late pupal stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		3
embryo 02-04hr		1
embryo 04-06hr		1
embryo 06-08hr		7
embryo 08-10hr		14
embryo 10-12hr		15
embryo 12-14hr		16
embryo 14-16hr		18
embryo 16-18hr		3
embryo 18-20hr		13
embryo 20-22hr		32
embryo 22-24hr		23
larva L1		11
larva L2		7
larva L3 12hr old		6
larva L3 puffstage 1-2		3
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		32
white prepupae new		27
white prepupae 12hr		26
white prepupae 24hr		24
pupae 2d postWPP		71
pupae 3d postWPP		17
pupae 4d postWPP		41
adult male 01day		6
adult male 05day		8
adult male 30day		7
adult female 01day		8
adult female 05day		5
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		3
embryo 02-04hr		1
embryo 04-06hr		1
embryo 06-08hr		7
embryo 08-10hr		14
embryo 10-12hr		15
embryo 12-14hr		16
embryo 14-16hr		18
embryo 16-18hr		3
embryo 18-20hr		13
embryo 20-22hr		(32)
embryo 22-24hr		23
larva L1		11
larva L2		7
larva L3 12hr old		6
larva L3 puffstage 1-2		3
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		(32)
white prepupae new		(27)
white prepupae 12hr		26
white prepupae 24hr		24
pupae 2d postWPP		(71)
pupae 3d postWPP		17
pupae 4d postWPP		(41)
adult male 01day		6
adult male 05day		8
adult male 30day		7
adult female 01day		8
adult female 05day		5
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		3
embryo 02-04hr		1
embryo 04-06hr		1
embryo 06-08hr		7
embryo 08-10hr		14
embryo 10-12hr		15
embryo 12-14hr		16
embryo 14-16hr		18
embryo 16-18hr		3
embryo 18-20hr		13
embryo 20-22hr		32
embryo 22-24hr		23
larva L1		11
larva L2		7
larva L3 12hr old		6
larva L3 puffstage 1-2		3
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		32
white prepupae new		27
white prepupae 12hr		26
white prepupae 24hr		24
pupae 2d postWPP		71
pupae 3d postWPP		17
pupae 4d postWPP		41
adult male 01day		6
adult male 05day		8
adult male 30day		7
adult female 01day		8
adult female 05day		5
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		3
embryo 02-04hr		1
embryo 04-06hr		1
embryo 06-08hr		7
embryo 08-10hr		14
embryo 10-12hr		15
embryo 12-14hr		16
embryo 14-16hr		18
embryo 16-18hr		3
embryo 18-20hr		13
embryo 20-22hr		32
embryo 22-24hr		23
larva L1		11
larva L2		7
larva L3 12hr old		6
larva L3 puffstage 1-2		3
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		32
white prepupae new		27
white prepupae 12hr		26
white prepupae 24hr		24
pupae 2d postWPP		71
pupae 3d postWPP		17
pupae 4d postWPP		41
adult male 01day		6
adult male 05day		8
adult male 30day		7
adult female 01day		8
adult female 05day		5
adult female 30day		6
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		3
embryo 02-04hr		1
embryo 04-06hr		1
embryo 06-08hr		7
embryo 08-10hr		14
embryo 10-12hr		15
embryo 12-14hr		16
embryo 14-16hr		18
embryo 16-18hr		3
embryo 18-20hr		13
embryo 20-22hr		32
embryo 22-24hr		23
larva L1		11
larva L2		7
larva L3 12hr old		6
larva L3 puffstage 1-2		3
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		32
white prepupae new		27
white prepupae 12hr		26
white prepupae 24hr		24
pupae 2d postWPP		71
pupae 3d postWPP		17
pupae 4d postWPP		41
adult male 01day		6
adult male 05day		8
adult male 30day		7
adult female 01day		8
adult female 05day		5
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		3
embryo 02-04hr		1
embryo 04-06hr		1
embryo 06-08hr		7
embryo 08-10hr		14
embryo 10-12hr		15
embryo 12-14hr		16
embryo 14-16hr		18
embryo 16-18hr		3
embryo 18-20hr		13
embryo 20-22hr		32
embryo 22-24hr		23
larva L1		11
larva L2		7
larva L3 12hr old		6
larva L3 puffstage 1-2		3
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		32
white prepupae new		27
white prepupae 12hr		26
white prepupae 24hr		24
pupae 2d postWPP		(71)
pupae 3d postWPP		17
pupae 4d postWPP		(41)
adult male 01day		6
adult male 05day		8
adult male 30day		7
adult female 01day		8
adult female 05day		5
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		3
embryo 02-04hr		1
embryo 04-06hr		1
embryo 06-08hr		7
embryo 08-10hr		14
embryo 10-12hr		15
embryo 12-14hr		16
embryo 14-16hr		18
embryo 16-18hr		3
embryo 18-20hr		13
embryo 20-22hr		32
embryo 22-24hr		23
larva L1		11
larva L2		7
larva L3 12hr old		6
larva L3 puffstage 1-2		3
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		32
white prepupae new		27
white prepupae 12hr		26
white prepupae 24hr		24
pupae 2d postWPP		71
pupae 3d postWPP		17
pupae 4d postWPP		41
adult male 01day		6
adult male 05day		8
adult male 30day		7
adult female 01day		8
adult female 05day		5
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		3
embryo 02-04hr		1
embryo 04-06hr		1
embryo 06-08hr		7
embryo 08-10hr		14
embryo 10-12hr		15
embryo 12-14hr		16
embryo 14-16hr		18
embryo 16-18hr		3
embryo 18-20hr		13
embryo 20-22hr		32
embryo 22-24hr		23
larva L1		11
larva L2		7
larva L3 12hr old		6
larva L3 puffstage 1-2		3
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		32
white prepupae new		27
white prepupae 12hr		26
white prepupae 24hr		24
pupae 2d postWPP		71
pupae 3d postWPP		17
pupae 4d postWPP		41
adult male 01day		6
adult male 05day		8
adult male 30day		7
adult female 01day		8
adult female 05day		5
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		9
Schneider line 2 Sg4		9
embryonic 1182-4H		8
embryonic GM2		7
embryonic Kc167		9
embryonic S1		15
embryonic S3		8
leg disc CME L1		8
wing disc CME-W2		8
wing disc ML-DmD8		4
wing disc ML-DmD9		7
wing disc ML-DmD16-c3		10
wing disc ML-DmD21		3
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		6
eye-antennal disc ML-DmD11		7
antennal disc ML-DmD20-c5		3
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		7
tumorous blood cells mbn2		15
ovary fGS/OSS		52
ovary OSC		2
ovary OSS		14
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		9
Schneider line 2 Sg4		9
embryonic 1182-4H		8
embryonic GM2		7
embryonic Kc167		9
embryonic S1		15
embryonic S3		8
leg disc CME L1		8
wing disc CME-W2		8
wing disc ML-DmD8		4
wing disc ML-DmD9		7
wing disc ML-DmD16-c3		10
wing disc ML-DmD21		3
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		6
eye-antennal disc ML-DmD11		7
antennal disc ML-DmD20-c5		3
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		7
tumorous blood cells mbn2		15
ovary fGS/OSS		(52)
ovary OSC		2
ovary OSS		14
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		9
Schneider line 2 Sg4		9
embryonic 1182-4H		8
embryonic GM2		7
embryonic Kc167		9
embryonic S1		15
embryonic S3		8
leg disc CME L1		8
wing disc CME-W2		8
wing disc ML-DmD8		4
wing disc ML-DmD9		7
wing disc ML-DmD16-c3		10
wing disc ML-DmD21		3
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		6
eye-antennal disc ML-DmD11		7
antennal disc ML-DmD20-c5		3
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		7
tumorous blood cells mbn2		15
ovary fGS/OSS		52
ovary OSC		2
ovary OSS		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		9
Schneider line 2 Sg4		9
embryonic 1182-4H		8
embryonic GM2		7
embryonic Kc167		9
embryonic S1		15
embryonic S3		8
leg disc CME L1		8
wing disc CME-W2		8
wing disc ML-DmD8		4
wing disc ML-DmD9		7
wing disc ML-DmD16-c3		10
wing disc ML-DmD21		3
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		6
eye-antennal disc ML-DmD11		7
antennal disc ML-DmD20-c5		3
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		7
tumorous blood cells mbn2		15
ovary fGS/OSS		52
ovary OSC		2
ovary OSS		14
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		9
Schneider line 2 Sg4		9
embryonic 1182-4H		8
embryonic GM2		7
embryonic Kc167		9
embryonic S1		15
embryonic S3		8
leg disc CME L1		8
wing disc CME-W2		8
wing disc ML-DmD8		4
wing disc ML-DmD9		7
wing disc ML-DmD16-c3		10
wing disc ML-DmD21		3
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		6
eye-antennal disc ML-DmD11		7
antennal disc ML-DmD20-c5		3
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		7
tumorous blood cells mbn2		15
ovary fGS/OSS		52
ovary OSC		2
ovary OSS		14
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		9
Schneider line 2 Sg4		9
embryonic 1182-4H		8
embryonic GM2		7
embryonic Kc167		9
embryonic S1		15
embryonic S3		8
leg disc CME L1		8
wing disc CME-W2		8
wing disc ML-DmD8		4
wing disc ML-DmD9		7
wing disc ML-DmD16-c3		10
wing disc ML-DmD21		3
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		6
eye-antennal disc ML-DmD11		7
antennal disc ML-DmD20-c5		3
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		7
tumorous blood cells mbn2		15
ovary fGS/OSS		(52)
ovary OSC		2
ovary OSS		14
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		9
Schneider line 2 Sg4		9
embryonic 1182-4H		8
embryonic GM2		7
embryonic Kc167		9
embryonic S1		15
embryonic S3		8
leg disc CME L1		8
wing disc CME-W2		8
wing disc ML-DmD8		4
wing disc ML-DmD9		7
wing disc ML-DmD16-c3		10
wing disc ML-DmD21		3
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		6
eye-antennal disc ML-DmD11		7
antennal disc ML-DmD20-c5		3
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		7
tumorous blood cells mbn2		15
ovary fGS/OSS		52
ovary OSC		2
ovary OSS		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		9
Schneider line 2 Sg4		9
embryonic 1182-4H		8
embryonic GM2		7
embryonic Kc167		9
embryonic S1		15
embryonic S3		8
leg disc CME L1		8
wing disc CME-W2		8
wing disc ML-DmD8		4
wing disc ML-DmD9		7
wing disc ML-DmD16-c3		10
wing disc ML-DmD21		3
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		6
eye-antennal disc ML-DmD11		7
antennal disc ML-DmD20-c5		3
mixed discs ML-DmD4-c1		2
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		7
tumorous blood cells mbn2		15
ovary fGS/OSS		52
ovary OSC		2
ovary OSS		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		23
cold shock, 4-day adult		19
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		8
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		9
Zinc 4.5 mM 48 hrs, 4-day adult		9
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		7
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		8
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		23
cold shock, 4-day adult		19
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		8
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		9
Zinc 4.5 mM 48 hrs, 4-day adult		9
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		7
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		8
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		23
cold shock, 4-day adult		19
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		8
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		9
Zinc 4.5 mM 48 hrs, 4-day adult		9
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		7
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		8
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		23
cold shock, 4-day adult		19
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		8
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		9
Zinc 4.5 mM 48 hrs, 4-day adult		9
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		7
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		8
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		23
cold shock, 4-day adult		19
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		8
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		9
Zinc 4.5 mM 48 hrs, 4-day adult		9
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		7
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		8
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		23
cold shock, 4-day adult		19
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		8
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		9
Zinc 4.5 mM 48 hrs, 4-day adult		9
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		7
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		8
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		23
cold shock, 4-day adult		19
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		8
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		9
Zinc 4.5 mM 48 hrs, 4-day adult		9
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		7
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		8
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		23
cold shock, 4-day adult		19
heat shock, 4-day adult		6
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		8
Copper 0.5 mM 12 hrs, larvae L3		8
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		9
Zinc 4.5 mM 48 hrs, 4-day adult		9
Ethanol 2.5% 3 hrs, larvae L3		8
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		3
Caffeine 1.5 mg/ml 4 hrs, larvae L3		3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		7
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		8
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

External Data & Images

Show all FlyExpress processed images for Eip75B

BDGP expression data

•

GM04985

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0000568

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG8127-RB;GM02640;CG8127-RC

FlyExpress - Embryonic expression images (BDGP data)

•

Stages(s) 1-3

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal

lethal | larval stage

Eip75B⁵

lethal | larval stage | recessive

Eip75B^Δ51

lethal | pharate adult stage

lethal | pharate adult stage (with Eip75B^A81)

Eip75B^Δ51

lethal | pharate adult stage (with Eip75B^Δ51)

Eip75B^A81

lethal | pupal stage

partially lethal - majority die | larval stage (with Eip75B^A81)

Eip75B^Δ51

partially lethal - majority die | larval stage (with Eip75B^Δ51)

Eip75B^A81

partially lethal - majority die | pharate adult stage (with Eip75B¹)

Eip75B^Δ51

partially lethal - majority die | pharate adult stage (with Eip75B^Δ51)

Eip75B¹

partially lethal - majority die | prepupal stage (with Eip75B^A81)

Eip75B^Δ51

partially lethal - majority die | prepupal stage (with Eip75B^Δ51)

Eip75B^A81

partially lethal - majority live | adult stage (with Eip75B¹)

Eip75B^Δ51

partially lethal - majority live | adult stage (with Eip75B^Δ51)

Eip75B¹

viable

viable (with Eip75B^Δ1)

Eip75B^Δ51

viable (with Eip75B^Δ51)

Eip75B^Δ1

Sterility

Allele

fertile

fertile (with Eip75B^Δ1)

Eip75B^Δ51

fertile (with Eip75B^Δ51)

Eip75B^Δ1

Other Phenotypes

Allele

developmental rate defective (with Eip75B^A81)

Eip75B^Δ51

developmental rate defective (with Eip75B^Δ51)

Eip75B^A81

developmental rate defective | larval stage, with Scer\GAL4^phm.PO

Eip75B^{A.Scer\UAS.cCa}

developmental rate defective | larval stage | recessive

Eip75B^Δ51

flightless (with Eip75B¹)

Eip75B^Δ51

flightless (with Eip75B^Δ51)

Eip75B¹

large body | larval stage, with Scer\GAL4^phm.PO

Eip75B^{A.Scer\UAS.cCa}

long lived

Eip75B^BG01345

starvation stress response defective | male limited

Eip75B^BG01345

uncoordinated (with Eip75B¹)

Eip75B^Δ51

uncoordinated (with Eip75B^Δ51)

Eip75B¹

visible, with Scer\GAL4^bbg-C96

Eip75B^d08364

Phenotype manifest in

Allele

egg | germline clone

Eip75B¹⁸

embryonic/larval anterior spiracle (with Eip75B^A81)

Eip75B^Δ51

embryonic/larval anterior spiracle (with Eip75B^Δ51)

Eip75B^A81

embryonic/larval midgut | embryonic stage

Eip75B¹⁸

eye (with Eip75B¹)

Eip75B^Δ51

eye (with Eip75B^Δ51)

Eip75B¹

follicle cell | germline clone

Eip75B¹⁸

germline cell | female | germline clone

Eip75B¹⁸

midgut chamber 3

Eip75B¹⁸

midgut constriction 1

Eip75B¹⁸

mouth hooks (with Eip75B^A81)

Eip75B^Δ51

mouth hooks (with Eip75B^Δ51)

Eip75B^A81

oocyte | germline clone

Eip75B¹⁸

wing, with Scer\GAL4^bbg-C96

Eip75B^d08364

Classical Alleles ( 125 )

For All Classical Alleles Show

Allele of Eip75B	Mutagen	Stocks	Known lesion
Eip75B⁰²		1	Yes
Eip75B⁰⁷⁰⁴¹	P-element activity	1	--
Eip75B¹	X ray gamma ray	1	Yes
Eip75B^5-HA-1089		1	--
Eip75B^5-HA-2025		1	--
Eip75B^5-HA-2054		1	--
Eip75B^A81	P-element activity	1	Yes
Eip75B^BG02576	P-element activity	1	--
Eip75B^c03760	piggyBac transposase	1	--
Eip75B^CB-6290-3		1	--
Eip75B^CB-6439-3		1	--
Eip75B^d00390	P-element activity	1	--
Eip75B^d01304	P-element activity	1	--
Eip75B^d02450	P-element activity	1	--
Eip75B^d05288	P-element activity	1	--
Eip75B^d06814	P-element activity	1	--
Eip75B^d07841	P-element activity	1	--
Eip75B^d08184	P-element activity	1	--
Eip75B^d08364	P-element activity	1	--
Eip75B^d09801	P-element activity	1	--
Eip75B^d10232	P-element activity	1	--
Eip75B^e00951	piggyBac transposase	1	--
Eip75B^e01229	piggyBac transposase	1	--
Eip75B^e03140	piggyBac transposase	1	--
Eip75B^e03676	piggyBac transposase	1	--
Eip75B^e03833	piggyBac transposase	1	--
Eip75B^e04407	piggyBac transposase	1	--
Eip75B^EY01971	P-element activity	1	Yes
Eip75B^EY20010	P-element activity	1	--
Eip75B^f01050	piggyBac transposase	1	--
Eip75B^f02837	piggyBac transposase	1	--
Eip75B^f03790	piggyBac transposase	1	--
Eip75B^f07630	piggyBac transposase	1	--
Eip75B^f07855	piggyBac transposase	1	--
Eip75B^KG00139	P-element activity	1	--
Eip75B^KG03025	P-element activity	1	--
Eip75B^KG04491	P-element activity	1	--
Eip75B^KG09026	P-element activity	1	--
Eip75B^MI03665		1	--
Eip75B^MI04192		1	--
Eip75B^MI04895		1	--
Eip75B^MI05153		1	--
Eip75B^NP0549		1	--
Eip75B^NP1349		1	--
Eip75B^NP2395		1	--
Eip75B^NP3429		1	--
Eip75B^NP3547		1	--
Eip75B^NP4646		1	--
Eip75B^NP5473		1	--
Eip75B^NP7019		1	--
Eip75B^Δ1	P-element activity	1	Yes
Eip75B^Δ51	P-element activity	1	Yes
Eip75B⁰⁰²²⁵	P-element activity	0	--
Eip75B⁰⁰⁶⁸³	P-element activity	0	--
Eip75B⁰¹¹⁵⁵	P-element activity	0	--
Eip75B⁰²⁰⁹³	P-element activity	0	--
Eip75B⁰²⁵⁴⁸	P-element activity	0	--
Eip75B⁰³²⁴⁷	P-element activity	0	--
Eip75B⁰³³³⁸	P-element activity	0	--
Eip75B⁰³⁵⁵⁴	P-element activity	0	--
Eip75B⁰⁴⁸⁷¹	P-element activity	0	--
Eip75B⁰⁵⁵³⁸	P-element activity	0	--
Eip75B⁰⁵⁸⁷⁰	P-element activity	0	--
Eip75B⁰⁷³⁴³	P-element activity	0	--
Eip75B⁰⁷⁷¹⁶	P-element activity	0	--
Eip75B¹⁰⁴⁵⁰	P-element activity	0	--
Eip75B¹⁰	ethyl methanesulfonate	0	--
Eip75B¹¹	ethyl methanesulfonate	0	--
Eip75B¹²	ethyl methanesulfonate	0	--
Eip75B¹³	ethyl methanesulfonate	0	--
Eip75B¹⁴	ethyl methanesulfonate	0	--
Eip75B¹⁵	ethyl methanesulfonate	0	--
Eip75B¹⁶	ethyl methanesulfonate	0	--
Eip75B¹⁷	ethyl methanesulfonate	0	--
Eip75B¹⁸	ethyl methanesulfonate	0	--
Eip75B¹⁹	ethyl methanesulfonate	0	--
Eip75B²⁰	ethyl methanesulfonate	0	Yes
Eip75B²¹	ethyl methanesulfonate	0	--
Eip75B²²	ethyl methanesulfonate	0	--
Eip75B²³	ethyl methanesulfonate	0	--
Eip75B²⁴	ethyl methanesulfonate	0	--
Eip75B²⁵	ethyl methanesulfonate	0	--
Eip75B²⁶	ethyl methanesulfonate	0	--
Eip75B²⁷	ethyl methanesulfonate	0	--
Eip75B²⁸	ethyl methanesulfonate	0	--
Eip75B²	X ray	0	Yes
Eip75B^333.15	P-element activity	0	--
Eip75B³	X ray	0	Yes
Eip75B⁴	X ray	0	Yes
Eip75B⁵	X ray	0	--
Eip75B⁶	ethyl methanesulfonate	0	--
Eip75B⁷²		0	--
Eip75B⁷	ethyl methanesulfonate	0	--
Eip75B⁸	ethyl methanesulfonate	0	--
Eip75B⁹	ethyl methanesulfonate	0	--
Eip75B^BG01345	P-element activity	0	--
Eip75B^BG02737	P-element activity	0	--
Eip75B^CB05160	P-element activity	0	Yes
Eip75B^d00617	P-element activity	0	--
Eip75B^d00623	P-element activity	0	--
Eip75B^d01769		0	--
Eip75B^d02032		0	--
Eip75B^d02186	P-element activity	0	--
Eip75B^d04010	P-element activity	0	--
Eip75B^d04941	P-element activity	0	--
Eip75B^d07962	P-element activity	0	--
Eip75B^d08027	P-element activity	0	--
Eip75B^d08901	P-element activity	0	--
Eip75B^DG04611	P-element activity	0	--
Eip75B^EP1121b	P-element activity	0	--
Eip75B^EP3380	P-element activity	0	--
Eip75B^EP3388	P-element activity	0	--
Eip75B^EP3401	P-element activity	0	--
Eip75B^EP3500	P-element activity	0	--
Eip75B^EY10168	P-element activity	0	--
Eip75B^EY14885	P-element activity	0	--
Eip75B^j11A6	P-element activity	0	--
Eip75B^j12E8	P-element activity	0	--
Eip75B^j3A6	P-element activity	0	--
Eip75B^j5E1	P-element activity	0	--
Eip75B^j6B3	P-element activity	0	--
Eip75B^L5242b	P-element activity	0	--
Eip75B^{MtnA.T:SV5\V5}		0	Yes
Eip75B^neo25	P-element activity	0	--
Eip75B^rH705	P-element activity	0	--

Alleles Carried on Transgenic Constructs ( 19 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Eip75B	Mutagen	Stocks	Known lesion
Eip75B^{hs.T:Scer\GAL4.T:Ivir\HA1}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	2	Yes
Eip75B^GD1434	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Eip75B^GLC01418	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Eip75B^HMS01530	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Eip75B^JF02257	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Eip75B^KK108982	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Eip75B^A.hs	in vitro construct - regulatory fusion	0	Yes
Eip75B^A.MtnA	in vitro construct - regulatory fusion	0	Yes
Eip75B^{A.Scer\UAS.cCa}	in vitro construct - regulatory fusion	0	Yes
Eip75B^B.hs	in vitro construct - regulatory fusion	0	Yes
Eip75B^B.MtnA	in vitro construct - regulatory fusion	0	Yes
Eip75B^dsRNA.A.cKa	in vitro construct - RNAi	0	Yes
Eip75B^dsRNA.B.cKa	in vitro construct - RNAi	0	Yes
Eip75B^dsRNA.cDa	in vitro construct - RNAi	0	Yes
Eip75B^dsRNA.cDb	in vitro construct - RNAi	0	Yes
Eip75B^HM05203	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
Eip75B^hs.PP	in vitro construct - regulatory fusion	0	Yes
Eip75B^hs.PW	in vitro construct - regulatory fusion	0	Yes
Eip75B^{LBD.hs.T:Saur\A,T:Zzzz\TAP,T:Zzzz\FLAG,T:Zzzz\His6}	in vitro construct - coding region fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(3L)W4

(BDGP Project Members, 1994-1999)

Df(3L)W10

(Spradling et al., 1999, Segraves and Hogness, 1984, BDGP Project Members, 1994-1999)

Df(3L)x48

(Bilder and Scott, 1995)

Not disrupted in

Df(3L)81k19

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(3L)Cat

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

heat-shock construct

P{hs-GAL4-Eip75B.LBD.HA}

GAL4 construct

P{hs-GAL4-Eip75B.LBD.HA}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

insertion of enhancer trap binary system

Gene Ontology: Function, Process & Cellular Component ( 19 unique terms )

Terms Based on Experimental Evidence ( 9 terms )

Molecular Function

CV term

References

DNA binding

inferred from direct assay

heme binding

inferred from direct assay

(Marvin et al., 2009)

Biological Process

CV term

antimicrobial humoral response

References

inferred from mutant phenotype

(Kleino et al., 2005)

ecdysis, chitin-based cuticle

inferred from mutant phenotype

molting cycle, chitin-based cuticle

inferred from mutant phenotype

regulation of ecdysteroid metabolic process

inferred from mutant phenotype

regulation of gene expression

inferred from direct assay

response to ecdysone

inferred from mutant phenotype

Cellular Component

CV term

References

nucleus

inferred from direct assay

Terms Based on Predictions or Assertions ( 12 terms )

Molecular Function

CV term

ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity

References

inferred from sequence or structural similarity with EMBL:U09504

(FlyBase, 1992-)

non-traceable author statement

(Swiss-Prot Project Members, 1990.1.1, Swiss-Prot Project Members, 1990.8.1, Swiss-Prot Project Members, 1990.8.1, Maglich et al., 2001)

sequence-specific DNA binding

inferred from electronic annotation with InterPro:IPR001628

sequence-specific DNA binding transcription factor activity

inferred from sequence or structural similarity with EMBL:U09504

(FlyBase, 1992-)

steroid hormone receptor activity

inferred from electronic annotation with InterPro:IPR000536, InterPro:IPR001723, InterPro:IPR008946

thyroid hormone receptor activity

inferred from electronic annotation with InterPro:IPR001728

zinc ion binding

inferred from electronic annotation with InterPro:IPR001628, InterPro:IPR013088

Biological Process

CV term

ecdysone-mediated induction of salivary gland cell autophagic cell death

References

non-traceable author statement

(Buszczak and Segraves, 2000)

molting cycle, chitin-based cuticle

non-traceable author statement

(Swiss-Prot Project Members, 1990.1.1, Swiss-Prot Project Members, 1990.8.1, Swiss-Prot Project Members, 1990.8.1)

oogenesis

non-traceable author statement

(Kozlova and Thummel, 2000)

regulation of transcription, DNA-dependent

inferred from electronic annotation with InterPro:IPR000536, InterPro:IPR001628, InterPro:IPR001723, InterPro:IPR001728, InterPro:IPR008946, InterPro:IPR013088

(Swiss-Prot Project Members, 1990.1.1)

non-traceable author statement

steroid hormone mediated signaling pathway

inferred from electronic annotation with InterPro:IPR000536, InterPro:IPR008946

Cellular Component

CV term

(Swiss-Prot Project Members, 1990.1.1, Swiss-Prot Project Members, 1990.8.1, Swiss-Prot Project Members, 1990.8.1)

References

nucleus

inferred from sequence or structural similarity with EMBL:U09504

(FlyBase, 1992-)

non-traceable author statement

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Eip75B allele

Gene

References

Eip75B^333.15

ph-p

(Faucheux et al., 2001)

Eip75B⁰⁷⁰⁴¹

(Carrera et al., 1998, Abrell et al., 2000)

External Data

BioGRID - A database of protein and genetic interactions

•

65284

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0000568

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

39999

Orthologs

OrthoDB Orthologs (39) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG69S6S0

Dipteran inclusive ortholog search

EOG61RQ35

Insect inclusive ortholog search

EOG6TB36R

Arthropod inclusive ortholog search

EOG62RBPQ

Metazoa inclusive ortholog search

EOG6XWTR9

Orthologs in Drosophila Species (EOG69S6S0)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA23402

Drosophila pseudoobscura pseudoobscura

Dpse\GA23401

Drosophila persimilis

Dper\GL22731

Drosophila willistoni

Dwil\GK20573

Drosophila virilis

Dvir\GJ13918

Drosophila virilis

Dvir\GJ13915

Drosophila mojavensis

Dmoj\GI13580

Drosophila grimshawi

Dgri\GH14445

Drosophila grimshawi

Dgri\GH14852

Orthologs in non-Drosophila Dipterans (EOG61RQ35)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\e75B

Anopheles gambiae

Malaria mosquito

Agam\AGAP012223

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ007963

Orthologs in non-Dipteran Insects (EOG6TB36R)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\E75

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG037152

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH17941

Atta cephalotes

Leafcutter ant

Acep\ACEP12737

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO17721

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL13001

Linepithema humile

Argentine ant

Lhum\LH20627

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB21797

Solenopsis invicta

Red fire ant

Sinv\SINV25420

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007773

Bombyx mori

Silkmoth

Bmor\BGIBMGA006839

Pediculus humanus

Human body louse

Phum\PHUM233230

Tribolium castaneum

Red flour beetle

Tcas\E75

Orthologs in non-Insect Arthropods (EOG62RBPQ)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\COI

Ixodes scapularis

Deer tick

Isca\ISCW000630

Orthologs in non-Arthropod Metazoa (EOG6XWTR9)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\nhr-85

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_017491gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_017492gn

Danio rerio

Zebrafish

Drer\pparaa

Danio rerio

Zebrafish

Drer\nr1d4b

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (8) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA23402

Drosophila willistoni

Dwil\GK20573

Drosophila virilis

Dvir\GJ13918

Drosophila mojavensis

Dmoj\GI13580

Stocks & Reagents

Stocks Listed in FlyBase ( 59 )

PBac{PB}Eip75B^c03760

VDRC

v108399

P{KK108982}VIE-260B

More stocks available...

Genomic Clones ( 3 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 249 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

DRSC - Results from RNAi screens.

•

FBgn0000568

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

39999

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: Eip75B anon-WO0172774.31 anon-WO0172774.32

Source for merge of: Eip75B BcDNA:GM02640

Source for merge of: Eip75B l(3)neo25

(Spradling et al., 1999)

Additional comments

Source for merge of Eip75B anon-WO0172774.31 anon-WO0172774.32 was sequence comparison (date:051113).

Source for merge of Eip75B BcDNA:GM02640 was a shared cDNA (date:030728).

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a greater than three-fold increase in AttA activity in response to heat-killed E.coli after ecdysone treatment in S2 cells.

(Kleino et al., 2005)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Mutations affecting isoform A cause a reduced ecdysteroid titer during larval development, resulting in developmental delays, developmental arrests, and molting defects. These defects are rescuable by ecdysone treatment.

Ecdysteroid-induced Eip75B expression amplifies or maintains ecdysteroid titer during larval development, ensuring proper temporal progression through the life cycle.

(Burke and Kadonaga, 1996)

Hr46 receptor represses the early genes activated by the late-larval ecdysone pulse and with the help of Eip75B, provides a temporal linkage between the two ecdysone responses by controlling the expression of ftz-f1.

(White et al., 1997)

One of a class of genes with TATA-less promoters that have a subset of the conserved DPE sequence.

Reduced dosage of the early puff genes Eip74EF and Eip75B reduces the size of the late puffs and increasing the dosage increases the sizes.

(Russell et al., 1996)

A fragment of a Eip75B cDNA has been used to clone a homologous gene from the wax moth Galleria mellonella.

(Jindra et al., 1994)

Ecdysteroid-regulated gene.

(Andres et al., 1993)

Eip75B protein binds to discrete sites on polytene chromosomes, correlating with early and late ecdysone-induced puffs.

(Hill et al., 1993)

Random cDNAs from subtracted libraries subjected to a rapid and sensitive screen for their ability to detect mRNAs induced by ecdysone in the presence of cycloheximide identified the ecdysone inducible gene Eip75B.

(Hurban and Thummel, 1993)

The function of br is required for the complete ecdysone induction of early mRNAs from Eip74EF, Eip75B and the broad complex itself.

(Karim et al., 1993)

Developmentally regulated peak of arginine kinase at pupal stage P3 is affected by altering copy number of Eip75B and br, but not Eip74EF.

(James and Collier, 1992)

Encodes an ecdysone-inducible gene associated with the early puff at polytene position 75B. Periodic expression occurs in response to ecdysone pulses occurring at specific times during development; regression of puff and reduction of Eip75B expression in third instar does not occur in the presence of cycloheximide, and presumably depends on ecdysone-induced gene products.

(Lindsley and Zimm, 1992)

Eip75B encodes two proteins that contain sequences homologous to the DNA-binding and hormone-binding domains of proteins in the steroid receptor superfamily.