FlyBase Gene Report: Dmel\Eno

General Information

Symbol

Dmel\Eno

Species

D. melanogaster

Name

Enolase

Annotation Symbol

CG17654

Feature Type

protein_coding_gene

FlyBase ID

FBgn0000579

Gene Model Status

Current

Stock Availability

18 publicly available

Enzyme Name (EC)

Phosphopyruvate hydratase (4.2.1.11)

Gene Summary

Enolase (Eno) encodes a phosphopyruvate hydratase involved in glucose homeostasis. [Date last reviewed: 2019-09-12] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Red Book
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

22B1-22B1

Sequence location

2L:1,724,768..1,729,636 [-]

Recombination map

2-3

RefSeq locus

NT_033779 REGION:1724768..1729636

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables magnesium ion binding

inferred from electronic annotation with InterPro:IPR000941, InterPro:IPR020809, InterPro:IPR020810, InterPro:IPR020811

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables phosphopyruvate hydratase activity

inferred from sequence or structural similarity with UniProtKB:P06733

(FlyBase Curators, 2008-)

inferred from biological aspect of ancestor with PANTHER:PTN000224401

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in glucose homeostasis

inferred from mutant phenotype

(Ugrankar et al., 2015)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in canonical glycolysis

inferred from sequence or structural similarity with UniProtKB:P06733

(FlyBase Curators, 2008-)

involved_in glycolytic process

inferred from biological aspect of ancestor with PANTHER:PTN000224401

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from high throughput direct assay

(Lee et al., 2014)

located_in plasma membrane

inferred from high throughput direct assay

(Lee et al., 2014)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of phosphopyruvate hydratase complex

inferred from sequence or structural similarity with UniProtKB:P06733

(FlyBase Curators, 2008-)

inferred from biological aspect of ancestor with PANTHER:PTN000224401

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

OTHER HYDRO-LYASES

Protein Family (UniProt)

Belongs to the enolase family. (P15007)

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

Predictions / Assertions

2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O (4.2.1.11)

Summaries

Gene Snapshot

Enolase (Eno) encodes a phosphopyruvate hydratase involved in glucose homeostasis. [Date last reviewed: 2019-09-12]

Gene Group (FlyBase)

OTHER HYDRO-LYASES -: The Other Hydro-lyases is a collection of Hydro-lyases that do not fit into any of the other major Hydro-lyase groups.

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Eno: Enolase

Structural gene for enolase [2-phospho-D-glycerate hydrolase (EC 4.2.1.11)].

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Eno for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P15007)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Eno-RA

FBtr0077909

NM_058073

1754

433

Eno-RB

FBtr0077905

NM_164431

2183

500

Eno-RC

FBtr0077907

NM_164432

2257

500

Eno-RD

FBtr0077908

NM_164433

2198

500

Eno-RE

FBtr0077906

NM_164434

2664

500

Eno-RF

FBtr0302115

NM_001169382

2561

433

Additional Transcript Data and Comments

Reported size (kB)

1.3 (sequence analysis)

(Bishop and Corces, 1990)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Eno-PA

FBpp0077575

46.7

433

6.52

NP_477421

AAN10458

Eno-PB

FBpp0077571

54.3

500

8.85

NP_722721

AAN10455

Eno-PC

FBpp0077573

54.3

500

8.85

NP_722722

AAN10456

Eno-PD

FBpp0077574

54.3

500

8.85

NP_722723

AAN10457

Eno-PE

FBpp0077572

54.3

500

8.85

NP_722724

AAF51344

Eno-PF

FBpp0291325

46.7

433

6.52

NP_001162853

ACZ94145

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

500 aa isoforms: Eno-PB, Eno-PC, Eno-PD, Eno-PE

433 aa isoforms: Eno-PA, Eno-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

433 (aa); 46.563 (kD predicted)

(Bishop and Corces, 1990)

Comments

External Data

Subunit Structure (UniProtKB)

Homodimer.

(UniProt, P15007)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Eno using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

wing disc

(Santaren et al., 1993)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010, Dorus et al., 2006)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from high throughput direct assay

(Lee et al., 2014)

located_in plasma membrane

inferred from high throughput direct assay

(Lee et al., 2014)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Eno in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Eno

View exonic expression by tissue for Dmel\Eno

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33351/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0000579

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0000579

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0000579

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG17654

Flygut - An atlas of the Drosophila adult midgut

FBgn0000579

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 14 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Eno

Transgenic constructs containing regulatory region of Eno

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(2L)Exel6005

(Ryder, 2004.4.26)

Df(2L)Exel7007

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Eno^CB02039

lethal, with Scer\GAL4^da.PU

Eno^KK100571

lethal - all die before end of P-stage

Eno^e01615

viable

Eno^EY02251

viable, with Scer\GAL4^nSyb.PS

Eno^KK100571

Sterility

Allele

fertile

Eno^EY02251

Other Phenotypes

Allele

abnormal body size | P-stage

Eno^e01615

abnormal locomotor behavior, with Scer\GAL4^repo.PU

Eno^KK100571

abnormal size | adult stage, with Scer\GAL4^eya.enh1+E+2

Eno^HMC05514

decreased size | adult stage, with Scer\GAL4^C-765

Eno^KK100571

flightless, with Scer\GAL4^repo.PU

Eno^KK100571

Phenotype manifest in

Allele

eye, with Scer\GAL4^eya.enh1+E+2

Eno^HMC05514

pupa

Eno^e01615

wing blade, with Scer\GAL4^C-765

Eno^KK100571

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\ENO1

13 of 15

Yes

Hsap\ENO2

12 of 15

Yes

Hsap\ENO3

12 of 15

Yes

Hsap\ENO4

3 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Eno2

13 of 15

Yes

Mmus\Eno1b

12 of 15

Yes

Mmus\Eno3

12 of 15

Yes

Mmus\Eno1

8 of 15

Yes

Mmus\Eno4

3 of 15

Yes

Rattus norvegicus (Norway rat) (6)

Rnor\Eno1

12 of 13

Yes

Rnor\Eno3

11 of 13

Yes

Rnor\LOC100911625

8 of 13

Yes

Rnor\Eno2

7 of 13

Yes

Rnor\Eno4

3 of 13

Yes

Rnor\RGD1559534

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (3)

Xtro\eno1

12 of 12

Yes

Xtro\eno4

3 of 12

Yes

Xtro\eno3

1 of 12

Yes

Danio rerio (Zebrafish) (5)

Drer\eno1a

13 of 15

Yes

Drer\eno1b

12 of 15

Yes

Drer\eno2

12 of 15

Yes

Drer\eno3

11 of 15

Yes

Drer\eno4

3 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\enol-1

12 of 15

Yes

Arabidopsis thaliana (thale-cress) (3)

Atha\ENO1

8 of 9

Yes

Atha\LOS2

3 of 9

Yes

Atha\ENOC

2 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\ENO1

13 of 15

Yes

Scer\ENO2

11 of 15

Yes

Scer\ERR2

11 of 15

Yes

Scer\ERR3

10 of 15

Yes

Scer\ERR1

7 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\eno101

10 of 12

Yes

Spom\eno102

7 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

13 of 15

12 of 15

12 of 15

GLYCOGEN STORAGE DISEASE XIII; GSD13

ENO4; enolase 4

3 of 15

131375

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Eno

x-ray crystallography, predetermined participant

(Sun et al., 2017)

Eno - 14-3-3ε

experimental knowledge based

(Guruharsha et al., 2011)

Eno - ATPsynCF6

experimental knowledge based

(Guruharsha et al., 2011)

Eno - ATPsynβ

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Adh

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Akap200

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Akr1B

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Ald1

experimental knowledge based

(Guruharsha et al., 2011)

Eno - AnxB10

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Argk1

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Bacc

experimental knowledge based

(Guruharsha et al., 2011)

Eno - CG10638

experimental knowledge based

(Guruharsha et al., 2011)

Eno - CG11267

experimental knowledge based

(Guruharsha et al., 2011)

Eno - CG14715

experimental knowledge based

(Guruharsha et al., 2011)

Eno - CG2852

experimental knowledge based

(Guruharsha et al., 2011)

Eno - CG4598

experimental knowledge based

(Guruharsha et al., 2011)

Eno - CG8036

experimental knowledge based

(Guruharsha et al., 2011)

Eno - CG8993

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Calr

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Chd64

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Cyp1

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Df31

experimental knowledge based

(Guruharsha et al., 2011)

Eno - EndoGI

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Fkbp12

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Fkbp14

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Gapdh2

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Gdi

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Gpdh1

experimental knowledge based

(Guruharsha et al., 2011)

Eno - GstE6

experimental knowledge based

(Guruharsha et al., 2011)

Eno - GstO3

experimental knowledge based

(Guruharsha et al., 2011)

Eno - GstS1

experimental knowledge based

(Guruharsha et al., 2011)

Eno - HINT1

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Hsc70Cb

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Inos

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Mdh2

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Mer

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Eno - Mlc1

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Mlc2

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Moe

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Nacα

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Nlp

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Nph

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Ntf-2

experimental knowledge based

(Guruharsha et al., 2011)

Eno - PCNA

experimental knowledge based

(Guruharsha et al., 2011)

Eno - PHGPx

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Pdi

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Pgd

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Pgk

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Pglym78

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Prosα1

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Prosβ5

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Prx2540-2

experimental knowledge based

(Guruharsha et al., 2011)

Eno - RhoGDI

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Scp1

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Sh3β

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Sod1

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Sod2

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Stip1

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Sumo

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Tctp

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Tm2

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Trx-2

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Trxr-1

experimental knowledge based

(Guruharsha et al., 2011)

Eno - Uch

experimental knowledge based

(Guruharsha et al., 2011)

Eno - aub

anti tag coimmunoprecipitation, cross-linking study, Identification by mass spectrometry

(Zheng et al., 2016)

Eno - awd

experimental knowledge based

(Guruharsha et al., 2011)

Eno - capt

experimental knowledge based

(Guruharsha et al., 2011)

Eno - chic

experimental knowledge based

(Guruharsha et al., 2011)

Eno - cib

experimental knowledge based

(Guruharsha et al., 2011)

Eno - dUTPase

experimental knowledge based

(Guruharsha et al., 2011)

Eno - eEF1δ

experimental knowledge based

(Guruharsha et al., 2011)

Eno - eEF2

experimental knowledge based

(Guruharsha et al., 2011)

Eno - eEF5

experimental knowledge based

(Guruharsha et al., 2011)

Eno - fabp

experimental knowledge based

(Guruharsha et al., 2011)

Eno - kdn

experimental knowledge based

(Guruharsha et al., 2011)

Eno - mAcon1

experimental knowledge based

(Guruharsha et al., 2011)

Eno - porin

experimental knowledge based

(Guruharsha et al., 2011)

Eno - regucalcin

experimental knowledge based

(Guruharsha et al., 2011)

Eno - scf

experimental knowledge based

(Guruharsha et al., 2011)

Eno - scu

experimental knowledge based

(Guruharsha et al., 2011)

Eno - tud

anti tag coimmunoprecipitation, cross-linking study, Identification by mass spectrometry

(Zheng et al., 2016)

Eno - yip2

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Homodimer.

(UniProt, P15007 )

Linkouts

BioGRID - A database of protein and genetic interactions.

59591

DroID - A comprehensive database of gene and protein interactions.

FBgn0000579

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0000579

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-3

Cytogenetic map

22B1-22B1

Sequence location

2L:1,724,768..1,729,636 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

22B1-22B1

Limits computationally determined from genome sequence between P{PZ}l(2)04111⁰⁴¹¹¹ and P{lacW}CG17646^k11021

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

22A-22A

(determined by in situ hybridisation)

(Bishop and Corces, 1990)

Experimentally Determined Recombination Data

Location

2-3

(FlyBase, 2016)

2-3.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (18)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

26300

y¹ v¹; P{TRiP.JF02070}attP2

17972

w¹¹¹⁸; PBac{RB}Eno^e01615/CyO

19078

w¹¹¹⁸; PBac{WH}Eno^f07543/CyO

19899

y¹ w^67c23; P{EPgy2}Eno^EY02251

22610

y¹ w^67c23; P{EPgy2}Eno^EY23161

13701

y¹ w^67c23; P{SUPor-P}Eno^KG01162

41647

y¹ v¹; P{TRiP.GL01229}attP2/TM3, Sb¹

Kyoto

124392

w¹¹¹⁸; P{RS3}Eno^UM-8172-3

126281

w¹¹¹⁸; P{RS5}Eno^5-SZ-4004

104167

w^*; P{GawB}Eno^NP2353 / CyO

VDRC

v330201

P{VSH330201}attP40

v110090

P{KK100571}VIE-260B

More stocks available...

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (299)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0000579

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0000579

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33351

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: Eno BEST:LD15491

(Rana, 2004.7.8)

Source for merge of: Eno anon-WO0153538.76

(FlyBase, 1996-)

Additional comments

Source for merge of Eno anon-WO0153538.76 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Other Comments

Identified in 2D gels of CMW W2 wing imaginal disc cell proteins.

(Santaren et al., 1993)

Structural gene for enolase (2-phospho-D-glycerate hydrolase).

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 81 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33351

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P15007

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P15007

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

E1JHR5

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P15007

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0000579

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33351/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0000579

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0000579

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0000579

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG17654

Flygut - An atlas of the Drosophila adult midgut

FBgn0000579

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0000579

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33351

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG17654

MARRVEL_MODEL - MARRVEL (model organism gene)

33351

modMine - A data warehouse for the modENCODE project

FBgn0000579

Linkouts

BioGRID - A database of protein and genetic interactions.

59591

DroID - A comprehensive database of gene and protein interactions.

FBgn0000579

DRSC - Results frm RNAi screens

FBgn0000579

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0000579

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

FlyMine - An integrated database for Drosophila genomics

FBgn0000579

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (protein-protein) - An integrated Molecular Interaction Database

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

BEST:LD15491

CG17654

(Meadows, 2017.1.11, Buescher et al., 2013, Jensen et al., 2013, Cammarato et al., 2011, Sun et al., 2011, Ni et al., 2010.12.1, Thornton et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009, Perkins et al., 2009.3.31, Reumer et al., 2009, Terhzaz et al., 2006, Nakahara et al., 2005, Karlsson et al., 2004, Vierstraete et al., 2004, Pile et al., 2003, Vierstraete et al., 2003)

CT32526

(Xie and Ding, 2000)

DmENO

(Sun et al., 2017)

ENOA

(Mushegian et al., 1998)

Eno

(Hunt and Demontis, 2022, Anqueira-González et al., 2021, Pang et al., 2021, Solana-Manrique et al., 2021, Li et al., 2020, Solana-Manrique et al., 2020, Deshpande and Meller, 2018, Park et al., 2018, Ugrankar et al., 2018, Sen et al., 2017, Transgenic RNAi Project members, 2017-, Elgart et al., 2016, Green et al., 2016, Cogni et al., 2015, Kwon et al., 2015, Ugrankar et al., 2015, Chen et al., 2014, de la Cova et al., 2014, Lavington et al., 2014, Lee et al., 2014, Luck et al., 2014, Mukherjee et al., 2014, Vinayagam et al., 2014, Buescher et al., 2013, Colinet et al., 2013, Kwon et al., 2013, Kwon et al., 2013, Nirala et al., 2013, O'Sullivan et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Eanes, 2011, Franco et al., 2011, Friedman et al., 2011, Kallappagoudar et al., 2010, Wasbrough et al., 2010, Reumer et al., 2009, Liu and Lehmann, 2008, Buszczak et al., 2007, Flowers et al., 2007, Beller et al., 2006, Dorus et al., 2006)

Eno2

(Harr and Schlotterer, 2000)

T18

(Vierstraete et al., 2004)

anon-WO0153538.76

eno

(Terhzaz et al., 2006)

Name Synonyms

Enolase

(Rice et al., 2020, Ding et al., 2018, Zheng et al., 2016, Volkenhoff et al., 2015, Felix et al., 2012, Cammarato et al., 2011, Anholt and Williams, 2010, Robinson et al., 2010, Reumer et al., 2009, Lindsay et al., 2008, Cho et al., 2005)

enolase

(Vinayagam et al., 2014, Buescher et al., 2013, Schriner et al., 2013, Sun et al., 2011)

phosphopyruvate hydratase

(Zhang et al., 2004)

Secondary FlyBase IDs

FBgn0045789
FBgn0045874

Datasets (8)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (8)

Result

Result Type

Title

scRNAseq_2020_Cattenoz_NI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from non-infested third instar larvae

scRNAseq_2020_Cattenoz_WI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from wasp-infested third instar larvae

scRNAseq_2020_Cho_NI72LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying

scRNAseq_2020_Cho_NI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying

scRNAseq_2020_Cho_WI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI96HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying

References (133)

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