HomeGene Dmel\Est-6
| General Information | ||||
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| Symbol | Dmel\Est-6 | Species | D. melanogaster | |
| Name | Esterase 6 | Annotation symbol | CG6917 | |
| Feature type | protein_coding_gene | FlyBase ID | FBgn0000592 | |
| Created / Updated | 2003-12-01/2003-12-01 | |||
| Genomic Location | ||||
| Chromosome (arm) | 3L | Recombination map | 3-35.9 | |
| Cytogenetic map | 69A1-69A1 | Sequence location | 3L:12,181,935..12,183,681 [+] | |
| Map ( GBrowse ) | 
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Summary Information
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Automatically generated summary
See sections below for more information | The gene Esterase 6 is referred to in FlyBase by the symbol Est-6 (CG6917, FBgn0000592). It has the cytological map location 69A1. Its sequence location is 3L:12181935..12183681. Its molecular function is described as carboxylesterase activity. It is involved in the biological processes: sperm competition; mating; ovulation; regulation of oviposition; sperm storage; courtship behavior; pheromone biosynthetic process; regulation of female receptivity, post-mating; sexual reproduction. 84 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. | |||
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Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
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| Gene/Allele symbols may differ from current usage | Est-6: Esterase 6
The structural gene for the nonspecific carboxylesterase, [EST-6 (EC 3.1.1.1)]. One of the ten positively
migrating esterases demonstrable with α-naphthyl acetate and
Fast Blue BB after starch gel electrophoresis of homogenates.
Molecular weight determinations on purified enzyme indicate
that the active enzyme is a 62,000 to 65,000 dalton glycoprotein monomer (Mane, Tepper, and Richmond, 1983, Biochem.
Genet. 21: 1019-40). Substrate specificities to different
esters explored by Danford and Beardmore (1979). Specific
EST-6 activity during development shows a prominant transient
peak during second larval instar and shows a male-specific
rise in activity beginning 36-48 hr after eclosion; males
reach level double that of females (Sheehan, Richmond, and
Cochrane, 1979, Insect Biochem. 9: 443-50); similar high levels found in male-like triploid intersexes (Aronshtam and
Kuzin, 1974, Zh. Obsch. 35: 926-33) and X/X;tra/tra (Johnson,
1964, Genetics 50: 259). High concentrations of EST-6 found
in ejaculatory duct; at mating, male level depleted and female
level enhanced; transfer of activity from male to Est-60
female detected early during copulation, prior to the transfer
of sperm, indicating that EST-6 is a component of seminal
fluid (Richmond, Gilbert, Sheehan, Gromko, and Butterworth,
1980, Science 207: 1483-85). Enzyme levels in flies carrying
different X chromosomes from natural populations in combination with constant autosomal complement vary suggesting X-linked modifiers (Tepper, Richmond, and Terry, 1981, Genetics
97: s103). In D. melanogaster X D. simulans hybrid males
that carry a D. melanogaster X chromosome, the level of the
D. melanogaster enzyme is reduced compared to that of the
D. simulans enzyme; hybrid females have equivalent activities
of the two enzymes (Korchkin, Aronshtam, and Matveeva, 1974,
Biochem. Genet. 12: 9-24). Electrophoretic mobility reduced
modestly from 1.10 to 1.08 for Est-6F and from 1.0 to 0.98 for
Est-6S by m(Est-6) on the third chromosome (Cochrane and Richmond, 1979, Biochem. Genet. 17: 167-83).
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Detailed Mapping Data
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| FlyBase Computed Cytological Location | ||||
Cytogenetic map Evidence for location 69A1-69A1
Limits computationally determined from genome sequence between P{PZ}rols08232&P{lacW}l(3)j2D3j2D3 and P{PZ}l(3)0508805088
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| Experimentally Determined Cytological Location | ||||
Cytogenetic map Notes References 69A1-69A3 (determined by in situ hybridisation)
69A-69A (determined by in situ hybridisation)
69A1-69A1 (determined by in situ hybridisation)
69A1-69A3 (determined by in situ hybridisation)
69A1-69A1 (determined by in situ hybridisation)
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| Experimentally Determined Recombination Data | ||||
| Location | 3-35.9 3-36.0 | |||
| Left of (cM) | ||||
| Right of (cM) | ||||
| Notes | ||||
| Molecular Map Data | ||||
Gene Order (in direction of increasing cytology)
References In direction of increasing cytology: Est-6+ Est-P+ Gene Order (overall orientation not stated) References Overall orientation not stated: anon-69Aa+ anon-69Ab+ Est-6+ Est-P+ anon-69Ac+ Overall orientation not stated: anon-69Aa+ anon-69Ab+ Est-6+ Est-P+ anon-69Ad- | ||||
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Gene Model & Products
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Please see the
GBrowse view of
Dmel\Est-6
for information on other features
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| Comments on Gene Model | ||||
DGC clone appears problematic: unspliced intron (RH59357) | ||||
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Transcript Data
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| Annotated Transcripts | ||||
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) | ||||
| Additional Transcript Data & Comments | ||||
| Reported size (kB) | 1.83, 1.68 (northern blot) 1.83, 1.68 (unknown) | |||
| Comments | ||||
| External Data | ||||
| Crossreferences | ||||
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Polypeptide Data
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| Annotated Polypeptides | ||||
Name FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
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| Additional Polypeptide Data & Comments | ||||
| Reported size (kD) | 548 (aa); 59.4 (kD predicted) 544 (aa) | |||
| Comments | ||||
| External Data | ||||
| Linkouts | PANTHER
- Protein classification by function, families, and pathways
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| Crossreferences | InterPro
domains - A database of protein families, domains, and functional sites
• Carboxylesterase, type B (IPR002018)
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Sequences Consistent with the Gene Model
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| DDBJ
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EMBL / GenBank | DNA sequence Protein sequence Name | |||
| UniProtKB/Swiss-Prot | ||||
| UniProtKB/TrEMBL | ||||
| Maps to | ||||
| Does NOT map to | ||||
| Identified with | ||||
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Mapped Features & Mutations
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Please see
GBrowse
or insertion reports for information on insertions of transgenic
constructs and features not listed here
Type Symbol & Location Additional Notes References rescue fragment Est-6[+t3.9.P1] 3L:12,180,745..12,184,694 comment=Position of restriction fragment on reference sequence inferred by FlyBase curator. evidence=experimental linked_to=HindIII-HindIII_rfrag rescue fragment Est-6[+t3.9.P7] 3L:12,180,745..12,184,694 comment=Position of restriction fragment on reference sequence inferred by FlyBase curator. evidence=experimental linked_to=HindIII-HindIII_rfrag | ||||
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External Data
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| Linkouts | DEDB
- Drosophila exon database: splicing graphs
• | |||
| Crossreferences | ||||
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Expression Data
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| FlyBase-Curated Data | ||||
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Transcript and Protein data | Please see the FlyBase Gene Expression Report for details of gene expression from the literature. | |||
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Summary of Transcript Expression
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Stage Tissue/Position
Reference
adult stage | male
embryonic stage-adult stage
pupal stage-adult stage | ||||
| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
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Summary of Polypeptide Expression
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Stage Tissue/Position
Reference
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| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
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Microarray Data
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Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) 
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External Data & Images
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| Linkouts | FLIGHT
- Cell culture data for RNAi and other high-throughput technologies
GEO (NCBI)
- Gene expression data: microarray and other high-throughput technologies
FlyExpress
- Embryonic expression images (BDGP data)
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Alleles & Phenotypes
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Summary of Allele Phenotypes
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Other Phenotypes | ||||
Stage(s)
11-12
Stage(s)
13-16