Gene Dmel\Est-6

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General Information

Symbol

Dmel\Est-6

Species

D. melanogaster

Name

Esterase 6

Annotation symbol

CG6917

Feature type

protein_coding_gene

FlyBase ID

FBgn0000592

Gene Model Status

Current

Stock availability

9 publicly available

Also Known As

Est6, Est, EST-5, Est-D

Genomic Location

Chromosome (arm)

Recombination map

3-35.9

Cytogenetic map

69A1-69A1

Sequence location

3L:12,181,935..12,183,805 [+]

Genomic Maps

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modENCODE GBrowse

Summary Information

Automatically generated summary

See sections below for more information

The gene Esterase 6 is referred to in FlyBase by the symbol Dmel\Est-6 (CG6917, FBgn0000592). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: carboxylesterase activity. There is experimental evidence that it is involved in the biological process: pheromone biosynthetic process; courtship behavior; multicellular organism reproduction. 84 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Carboxylesterase type B, active site; Carboxylesterase type B, conserved site; Carboxylesterase, type B. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of extremely low expression. Peak expression observed in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, adult crop, adult heart, adult fat body, adult salivary gland, adult spermathecae, adult male accessory gland, adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult central nervous system, adult hindgut, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1631224_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Est-6. Gene sequence location is 3L:12181935..12183805.

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

Est-6: Esterase 6

The structural gene for the nonspecific carboxylesterase, [EST-6 (EC 3.1.1.1)]. One of the ten positively migrating esterases demonstrable with α-naphthyl acetate and Fast Blue BB after starch gel electrophoresis of homogenates. Molecular weight determinations on purified enzyme indicate that the active enzyme is a 62,000 to 65,000 dalton glycoprotein monomer (Mane, Tepper, and Richmond, 1983, Biochem. Genet. 21: 1019-40). Substrate specificities to different esters explored by Danford and Beardmore (1979). Specific EST-6 activity during development shows a prominant transient peak during second larval instar and shows a male-specific rise in activity beginning 36-48 hr after eclosion; males reach level double that of females (Sheehan, Richmond, and Cochrane, 1979, Insect Biochem. 9: 443-50); similar high levels found in male-like triploid intersexes (Aronshtam and Kuzin, 1974, Zh. Obsch. 35: 926-33) and X/X;tra/tra (Johnson, 1964, Genetics 50: 259). High concentrations of EST-6 found in ejaculatory duct; at mating, male level depleted and female level enhanced; transfer of activity from male to Est-60 female detected early during copulation, prior to the transfer of sperm, indicating that EST-6 is a component of seminal fluid (Richmond, Gilbert, Sheehan, Gromko, and Butterworth, 1980, Science 207: 1483-85). Enzyme levels in flies carrying different X chromosomes from natural populations in combination with constant autosomal complement vary suggesting X-linked modifiers (Tepper, Richmond, and Terry, 1981, Genetics 97: s103). In D. melanogaster X D. simulans hybrid males that carry a D. melanogaster X chromosome, the level of the D. melanogaster enzyme is reduced compared to that of the D. simulans enzyme; hybrid females have equivalent activities of the two enzymes (Korchkin, Aronshtam, and Matveeva, 1974, Biochem. Genet. 12: 9-24). Electrophoretic mobility reduced modestly from 1.10 to 1.08 for Est-6F and from 1.0 to 0.98 for Est-6S by m(Est-6) on the third chromosome (Cochrane and Richmond, 1979, Biochem. Genet. 17: 167-83).

Recent Updates

Description

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FB2011_10

FB2012_01

Sequence features

BKN31421

HFA11162

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

69A1-69A1

Limits computationally determined from genome sequence between P{PZ}rols⁰⁸²³²&P{lacW}l(3)j2D3^j2D3 and P{PZ}l(3)05088⁰⁵⁰⁸⁸

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

69A1-69A3