FlyBase Gene Report: Dmel\e(y)1

(Fisher et al., 2012)

embryonic stage 7 -- 8

(Fisher et al., 2012)

embryonic stage 9 -- 10

(Fisher et al., 2012)

embryonic stage 11 -- 12

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | faint | ubiquitous

(Fisher et al., 2012)

transcription factor TFIID complex

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

testis

(Metcalf and Wassarman, 2007)

male accessory gland

(Metcalf and Wassarman, 2007)

seminal vesicle

(Metcalf and Wassarman, 2007)

spermatocyte

(Metcalf and Wassarman, 2007)

Additional Descriptive Data

e(y)1 protein was isolated from nuclear extracts.

(Goodrich et al., 1993)

Marker for

Subcellular Localization

CV Term

Evidence

References

nucleus

inferred from direct assay

(Weake et al., 2011, Guelman et al., 2006, Wright et al., 2006, Hansen and Tjian, 1995, Goodrich et al., 1993)

SAGA complex

inferred from direct assay

(Weake et al., 2011, Guelman et al., 2006)

inferred from physical interaction with FLYBASE:Ada3; FB:FBgn0030891

(Kusch et al., 2003)

inferred from physical interaction with FLYBASE:Ada2b; FB:FBgn0037555

(Kusch et al., 2003)

inferred from physical interaction with FLYBASE:Spt3; FB:FBgn0037981

(Kusch et al., 2003)

inferred from physical interaction with FLYBASE:Taf4; FB:FBgn0010280

(Wright et al., 2006)

inferred from physical interaction with FLYBASE:Taf6; FB:FBgn0010417

(Wright et al., 2006)

inferred from direct assay

(Georgieva et al., 2001, Hansen and Tjian, 1995)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\e(y)1 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\e(y)1

View exonic expression by tissue for Dmel\e(y)1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0000617

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0000617

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG6474-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6474

Flygut - An atlas of the Drosophila adult midgut

FBgn0000617

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of e(y)1

Transgenic constructs containing regulatory region of e(y)1

Deletions and Duplications ( 2 )

Duplicated in

Dp(1;3)DC335

(Xie et al., 2014)

Dp(1;3)DC336

(Xie et al., 2014)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of larval stage

e(y)1¹⁹⁰

lethal - all die before end of prepupal stage

viable

e(y)1¹

Sterility

Allele

female fertile

e(y)1^P1

female sterile | recessive

Other Phenotypes

Allele

visible, with Scer\GAL4^bbg-C96

e(y)1^{dsRNA.UAS.cUa}

visible | male | recessive

e(y)1^P1

visible | recessive | somatic clone

e(y)1¹⁹⁰

wild-type

e(y)1^+tSa

Phenotype manifest in

Allele

embryonic/larval fat body | wandering third instar larval stage

embryonic/larval fat body | wandering third instar larval stage, with Scer\GAL4^ppl.PP

e(y)1^HMS00336

lipid droplet | wandering third instar larval stage

lipid droplet | wandering third instar larval stage, with Scer\GAL4^ppl.PP

e(y)1^HMS00336

macrochaeta

e(y)1^P1

oocyte

e(y)1¹

ovary

e(y)1¹

wing margin, with Scer\GAL4^bbg-C96

e(y)1^{dsRNA.UAS.cUa}

wing margin | somatic clone

e(y)1¹⁹⁰

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\TAF9B

12 of 15

Yes

Hsap\TAF9

12 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Taf9b

13 of 15

Yes

Mmus\Taf9

10 of 15

Yes

Mmus\Ak6

1 of 15

Rattus norvegicus (Norway rat) (2)

Rnor\Taf9b

8 of 13

Yes

Rnor\Taf9

8 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\taf9b

7 of 12

Yes

Danio rerio (Zebrafish) (1)

Drer\taf9

12 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\taf-9

11 of 15

Yes

Arabidopsis thaliana (thale-cress) (1)

Atha\TAFII21

6 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\TAF9

9 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\taf9

7 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190ESM )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA26884

Drosophila persimilis

Dper\GL14751

Drosophila willistoni

Dwil\GK25325

Drosophila virilis

Dvir\GJ23848

Drosophila mojavensis

Dmoj\GI22602

Drosophila grimshawi

Dgri\GH19399

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150ARH )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17010170

Mayetiola destructor

Hessian fly

Mdes\Mdes013526

Mayetiola destructor

Hessian fly

Mdes\Mdes010316

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL006789

Anopheles darlingi

American malaria mosquito

Adar\ADAC008615

Anopheles gambiae

Malaria mosquito

Agam\AGAP003094

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ001398

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0H50 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA007359

Danaus plexippus

Monarch butterfly

Dple\DPOGS213170-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL002988

Apis florea

Little honeybee

Aflo\2524053620

Apis mellifera

Western honey bee

Amel\GB52516

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP21149

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP21143

Bombus terrestris

Buff-tailed bumblebee

Bter\15213772

Bombus terrestris

Buff-tailed bumblebee

Bter\15213765

Linepithema humile

Argentine ant

Lhum\LH18010

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002404

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG002195

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG002199

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_02652

Tribolium castaneum

Red flour beetle

Tcas\TC004292

Pediculus humanus

Human body louse

Phum\PHUM076550

Rhodnius prolixus

Kissing bug

Rpro\RPRC012661

Rhodnius prolixus

Kissing bug

Rpro\RPRC004922

Cimex lectularius

Bed bug

Clec\CLEC001914

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI060797

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI002736

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI071209

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI30466

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI073872

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI085994

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI43415

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_15908

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0H6J )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR008609

Ixodes scapularis

Black-legged tick

Isca\ISCW005230

Ixodes scapularis

Black-legged tick

Isca\ISCW002952

Ixodes scapularis

Black-legged tick

Isca\ISCW012450

Stegodyphus mimosarum

African social velvet spider

Smim\KFM65977.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur23g01020

Tetranychus urticae

Two-spotted spider mite

Turt\tetur23g01040

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_303843

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0RPE )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Taf9

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000013825

Gallus gallus

Domestic chicken

Ggal\TAF9

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (1)

Gene Symbol

Score

Source

Alignment

Ak6

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TAF9B; TATA-box binding protein associated factor 9b

12 of 15

300754

TAF9; TATA-box binding protein associated factor 9

12 of 15

600822

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

e(y)1 - Ada1-2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2009)

e(y)1 - Ada2a

anti tag coimmunoprecipitation, peptide massfingerprinting, anti bait coimmunoprecipitation, western blot, ion exchange chromatography, molecular sieving

(Muratoglu et al., 2003)

e(y)1 - Ada2b

(Weake et al., 2011, Weake et al., 2009, Kusch et al., 2003, Muratoglu et al., 2003)

e(y)1 - Ada3

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kusch et al., 2003)

e(y)1 - Atxn7

(Mohan et al., 2014)

e(y)1 - Bap60

ion exchange chromatography, molecular sieving, western blot

(Vorobyeva et al., 2009)

e(y)1 - CG4962

ion exchange chromatography, molecular sieving, western blot

(Guruharsha et al., 2011)

e(y)1 - Gcn5

(Muratoglu et al., 2003)

e(y)1 - His2B:CG33868

(Guruharsha et al., 2011)

e(y)1 - His4r

anti tag coimmunoprecipitation, anti tag western blot

(Guruharsha et al., 2011)

e(y)1 - N

(Xie et al., 2014)

e(y)1 - Nplp2

(Guruharsha et al., 2011)

e(y)1 - Pc

(Saurin et al., 2001)

e(y)1 - Psc

anti tag coimmunoprecipitation, peptide massfingerprinting, pull down, molecular weight estimation by staining

(Saurin et al., 2001)

e(y)1 - Saf6

(Weake et al., 2009)

e(y)1 - Sf3b5

anti tag coimmunoprecipitation, peptide massfingerprinting

(Stegeman et al., 2016)

e(y)1 - Sgf29

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2009)

e(y)1 - Spt20

anti tag coimmunoprecipitation, peptide massfingerprinting

(Stegeman et al., 2016)

e(y)1 - Spt3

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, peptide massfingerprinting

(Stegeman et al., 2016, Kusch et al., 2003)

e(y)1 - Su(H)

anti tag coimmunoprecipitation, anti tag western blot

(Xie et al., 2014)

e(y)1 - Taf1

anti bait coimmunoprecipitation, western blot, ion exchange chromatography, molecular sieving, affinity chromatography technology

(Vorobyeva et al., 2009, Santoso and Kadonaga, 2006, Goodrich et al., 1993)

e(y)1 - Taf10

(Muratoglu et al., 2003, Georgieva et al., 2001)

e(y)1 - Taf10b

(Muratoglu et al., 2003)

e(y)1 - Taf11

(Yokomori et al., 1993)

e(y)1 - Taf12

anti bait coimmunoprecipitation, western blot, ion exchange chromatography, molecular sieving

(Yokomori et al., 1993)

e(y)1 - Taf4

(Vorobyeva et al., 2009, Wright et al., 2006, Muratoglu et al., 2003, Saurin et al., 2001)

e(y)1 - Taf5

pull down, anti tag coimmunoprecipitation, molecular weight estimation by staining, anti bait coimmunoprecipitation, western blot, peptide massfingerprinting

(Saurin et al., 2001)

e(y)1 - Taf6

(Weake et al., 2009, Wright et al., 2006, Goodrich et al., 1993)

e(y)1 - Tbp

anti bait coimmunoprecipitation, molecular weight estimation by staining, western blot, ion exchange chromatography, molecular sieving

(Vorobyeva et al., 2009, Georgieva et al., 2001, Saurin et al., 2001, Chen et al., 1994, Verrijzer et al., 1994, Goodrich et al., 1993, Yokomori et al., 1993)

e(y)1 - TfIIB

pull down, western blot

(Goodrich et al., 1993)

e(y)1 - apolpp

(Guruharsha et al., 2011)

e(y)1 - e(y)2

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, ion exchange chromatography, molecular sieving, Identification by mass spectrometry

(Georgieva et al., 2001)

e(y)1 - e(y)3

(Vorobyeva et al., 2009, Vorobyeva et al., 2008)

e(y)1 - mor

anti bait coimmunoprecipitation, western blot, ion exchange chromatography, molecular sieving

(Vorobyeva et al., 2009)

e(y)1 - ph-p

anti tag coimmunoprecipitation, ion exchange chromatography, molecular sieving, western blot, anti bait coimmunoprecipitation, peptide massfingerprinting

(Saurin et al., 2001)

e(y)1 - polybromo

ion exchange chromatography, molecular sieving, western blot, anti bait coimmunoprecipitation

(Vorobyeva et al., 2009)

e(y)1 - wda

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2009)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

Su(H)

(Xie et al., 2014)

w|z|e(y)1

enhanceable

su(Hw)

(Soldatov et al., 1999)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Xie et al., 2014)

suppressible

(Gause et al., 1996)

su(Hw)|w|z

enhanceable

(Soldatov et al., 1999)

enhanceable

(Soldatov et al., 1999, Georgiev, 1994)

w|z

enhanceable

(Soldatov et al., 1999, Georgiev, 1994)

enhanceable

(Soldatov et al., 1999, Georgiev and Gerasimova, 1989)

enhanceable