lune, lun, Eygone, BK27
Pax family transcription factor that acts as a transcriptional repressor - promotes cell proliferation in the larval eye disc through activation of Jak/STAT pathway - plays both positive and negative roles in head vortex development
Gene model reviewed during 5.50
2.655 (longest cDNA)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eyg using the Feature Mapper tool.
eyg transcripts are first detected at embryonic stage 9 in the salivary gland precursor and a small group of cells in the dorsal head. The salivary gland precursor expression ends after embryonic development and is reinitiated later. At stage 10, transcripts are detected in the posterior spiracles and in a cluster of cells at the anterior edge of each thoracic and abdominal segment. By stage 12, expression is observed in the larval antennal organ and the leg disc primordium. In later stages, transcripts are found in the presumptive eye-antennal imaginal disc. In larvae, transcripts are observed at multiple sites. They are present in the medial and distal regions of the antennal disc and anterior to the morphogenetic furrow in eye discs in a narrow domain of cells that straddles the dorsal-ventral boundary. In wing discs, transcripts are expressed broadly in the notal region and in two discrete regions in the presumptive wing. eyg transcripts are also found in leg discs and in anterior duct cells of the salivary gland. This pattern of expression is nearly identical to that of toe and differs mainly in the persistance of expression in the salivary gland precursors.
Eye disc primordia express eyg in embryos. Expression of eyg in eye discs is detected at least as early as late second instar. eyg transcript is detected in the anterior of early larval eye discs and is strongly expressed dorsally and anterior to the morphogenetic furrow in late eye discs. Expression also occurs in the central region of antennal discs, the anterior notum, dorsal hinge and a region of the posterior periphery in the wing disc and in several restricted areas of the leg disc.
eyg is expressed in the salivary gland placode from stage 11. Expression is uniform at first in the placode and later fades in some areas such that a crescent pattern is formed. Expression is also observed in the subantennal region of the head and in a segmentally repeated pattern in the trunk. The segmental pattern begins as a single cell in each segment and develops into a ventrally located patch that fades to a narrow stripe during germ band retraction. Expression is also observed in the region around the posterior spiracle.
GBrowse - Visual display of RNA-Seq signalsView Dmel\eyg in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: eyg CG10488
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
eyg is essential for eye growth but is dispensible for specification of the eye.
Most metazoan homeodomains share a preference for the TAAT motif, they can differ from each other in their preference for the bases immediately 3' to this core. This preference is determined, in part, by the identity of amino acid position 50. Because homeodomain sequences have been identified that possess at least 10 different amino acids at position 50 it is investigated whether multiple DNA binding specificities can be conferred by changing this position to a variety of amino acid side chains.
Ives, 20th July 1940.