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General Information
Symbol
Dmel\eyg
Species
D. melanogaster
Name
eyegone
Annotation Symbol
CG10488
Feature Type
FlyBase ID
FBgn0000625
Gene Model Status
Stock Availability
Gene Snapshot
eyegone (eyg) encodes a Pax family transcription factor that acts as a transcriptional repressor. In eye development, the product of eyg promotes cell proliferation in the larval eye disc through activation of Jak/STAT pathway. It also plays both positive and negative roles in head vortex development. [Date last reviewed: 2019-03-07]
Also Known As

lune, lun, BK27, Eygone

Key Links
Genomic Location
Cytogenetic map
Sequence location
3L:12,462,537..12,468,021 [-]
Recombination map

3-38

RefSeq locus
NT_037436 REGION:12462537..12468021
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Summaries
Gene Group (FlyBase)
PAIRED HOMEOBOX TRANSCRIPTION FACTORS -
Paired homeobox transcription factors are sequence-specific DNA binding proteins that regulate transcription. They have a characteristic paired domain in association with the homeodomain. (Adapted from FBrf0135231 and PMID:1672661).
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
eyg: eye gone
Eyes and head much smaller than normal. Considerable pupal mortality, probably from inability to push open pupa cases. Adults normal in viability and productivity. Character subject to genetic modifiers and possibly environmental influences. Expression varies from complete absence of facets to formation of about 100 facets. Homozygotes show reduced larval and pupal survival (Inoue, 1980, DIS 55: 206). Lethal in combination with ey; phenotype described under ey [Hunt, 1970, Genet. Res. 15: 29-34 (fig.)]. RK2.
Summary (Interactive Fly)

Pax family transcription factor that acts as a transcriptional repressor - promotes cell proliferation in the larval eye disc through activation of Jak/STAT pathway - plays both positive and negative roles in head vortex development

Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\eyg or the JBrowse view of Dmel\eyg for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0075979
2691
670
FBtr0100170
2624
670
FBtr0339921
3148
670
Additional Transcript Data and Comments
Reported size (kB)

2.655 (longest cDNA)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0075711
70.1
670
8.11
FBpp0099525
70.1
670
8.11
FBpp0308953
70.1
670
8.11
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

670 aa isoforms: eyg-PA, eyg-PB, eyg-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eyg using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (12 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
Biological Process (8 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
eye disc

Comment: late second/early third instar

eye disc | anterior

Comment: late second/early third instar

Additional Descriptive Data

eyg transcripts are first detected at embryonic stage 9 in the salivary gland precursor and a small group of cells in the dorsal head. The salivary gland precursor expression ends after embryonic development and is reinitiated later. At stage 10, transcripts are detected in the posterior spiracles and in a cluster of cells at the anterior edge of each thoracic and abdominal segment. By stage 12, expression is observed in the larval antennal organ and the leg disc primordium. In later stages, transcripts are found in the presumptive eye-antennal imaginal disc. In larvae, transcripts are observed at multiple sites. They are present in the medial and distal regions of the antennal disc and anterior to the morphogenetic furrow in eye discs in a narrow domain of cells that straddles the dorsal-ventral boundary. In wing discs, transcripts are expressed broadly in the notal region and in two discrete regions in the presumptive wing. eyg transcripts are also found in leg discs and in anterior duct cells of the salivary gland. This pattern of expression is nearly identical to that of toe and differs mainly in the persistance of expression in the salivary gland precursors.

Eye disc primordia express eyg in embryos. Expression of eyg in eye discs is detected at least as early as late second instar. eyg transcript is detected in the anterior of early larval eye discs and is strongly expressed dorsally and anterior to the morphogenetic furrow in late eye discs. Expression also occurs in the central region of antennal discs, the anterior notum, dorsal hinge and a region of the posterior periphery in the wing disc and in several restricted areas of the leg disc.

eyg is expressed in the salivary gland placode from stage 11. Expression is uniform at first in the placode and later fades in some areas such that a crescent pattern is formed. Expression is also observed in the subantennal region of the head and in a segmentally repeated pattern in the trunk. The segmental pattern begins as a single cell in each segment and develops into a ventrally located patch that fades to a narrow stripe during germ band retraction. Expression is also observed in the region around the posterior spiracle.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

eyg protein can first be detected in third instar wing discs and is restricted to the anterior compartment in a region destined to become the anterior central region of the mesothorax.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\eyg in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 28 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 33 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of eyg
Transgenic constructs containing regulatory region of eyg
Deletions and Duplications ( 21 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
macrochaeta & scutum, with Scer\GAL4455.2
macrochaeta & scutum, with Scer\GAL4MD-638
macrochaeta & scutum, with Scer\GAL4MS248
macrochaeta & scutum, with Scer\GAL4pnr-MD237
macrochaeta & scutum, with Scer\GAL4ush-MD751
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (9)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
1 of 15
Yes
Yes
 
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
No
1 of 15
Yes
No
 
1 of 15
Yes
No
1 of 15
Yes
No
 
1 of 15
Yes
No
 
1 of 15
Yes
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (12)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
No
1 of 15
Yes
No
 
1 of 15
Yes
No
1 of 15
Yes
No
 
1 of 15
Yes
No
1 of 15
Yes
No
 
Rattus norvegicus (Norway rat) (7)
1 of 13
Yes
Yes
1 of 13
Yes
Yes
1 of 13
Yes
No
1 of 13
Yes
No
1 of 13
Yes
No
1 of 13
Yes
No
1 of 13
Yes
No
Xenopus tropicalis (Western clawed frog) (9)
1 of 12
Yes
Yes
1 of 12
Yes
Yes
1 of 12
Yes
Yes
1 of 12
Yes
No
1 of 12
Yes
No
1 of 12
Yes
No
1 of 12
Yes
No
1 of 12
Yes
No
1 of 12
Yes
Yes
Danio rerio (Zebrafish) (11)
1 of 15
Yes
Yes
1 of 15
Yes
Yes
1 of 15
Yes
No
1 of 15
Yes
No
1 of 15
Yes
No
1 of 15
Yes
No
1 of 15
Yes
No
1 of 15
Yes
No
1 of 15
Yes
No
1 of 15
Yes
No
1 of 15
Yes
No
Caenorhabditis elegans (Nematode, roundworm) (8)
7 of 15
Yes
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190987 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091506AU )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0K12 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0K6X )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G09JE )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (9)
5 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
2 of 10
2 of 10
2 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    RNA-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    suppressible
    suppressible
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3L
    Recombination map

    3-38

    Cytogenetic map
    Sequence location
    3L:12,462,537..12,468,021 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    69C2-69C2
    Limits computationally determined from genome sequence between P{PZ}l(3)0508805088 and P{PZ}l(3)0692406924
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Left of (cM)
    Notes
    Stocks and Reagents
    Stocks (18)
    Genomic Clones (18)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (4)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of

      Source for identity of: eyg CG10488

      Source for database merge of
      Additional comments
      Other Comments

      eyg induces expression of dac.

      The DNA binding activity of eyg relies on the L, RED and HD domains.

      RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

      Loss of N signaling in the eye can be compensated by overexpressing eyg, whereas loss of eyg function blocks effects of N signaling in the eye.

      Regulation of eyg by N involves Dl and Ser and requires the activity of Su(H) but is independent of E(spl)-mediated repression of genes.

      eyg is essential for eye growth but is dispensible for specification of the eye.

      eyg and ey act co-operatively in promoting eye development.

      eyg is required for embryonic salivary duct development.

      lune is stated to be the same as eyg demonstrated by lune rescue of the eyg mutant phenotype. There are no mutations of lune so allelism cannot be tested.

      Most metazoan homeodomains share a preference for the TAAT motif, they can differ from each other in their preference for the bases immediately 3' to this core. This preference is determined, in part, by the identity of amino acid position 50. Because homeodomain sequences have been identified that possess at least 10 different amino acids at position 50 it is investigated whether multiple DNA binding specificities can be conferred by changing this position to a variety of amino acid side chains.

      Origin and Etymology
      Discoverer

      Ives, 20th July 1940.

      Etymology
      Identification
      External Crossreferences and Linkouts ( 56 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyMine - An integrated database for Drosophila genomics
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Synonyms and Secondary IDs (14)
      Reported As
      Symbol Synonym
      eyg
      (Chai et al., 2019, Gaspar et al., 2019, Shokri et al., 2019, Kumar, 2018, Transgenic RNAi Project members, 2017-, Zhu et al., 2017, Bürglin and Affolter, 2016, Sarov et al., 2016, Rodriguez-Fernandez and Dell'Angelica, 2015, Schertel et al., 2015, Tanaka-Matakatsu et al., 2015, Liang et al., 2014, Luck et al., 2014, Ibrahim et al., 2013, Yu et al., 2013, Del Signore et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Salvany et al., 2012, Spokony and White, 2012.5.22, Chatterjee et al., 2011, Chopra et al., 2011, Datta et al., 2011, Devineni et al., 2011, Friedrich and Caravas, 2011, Gehring, 2011, McNeil et al., 2011, Vallejo et al., 2011, Blanco et al., 2010, Estella and Mann, 2010, Kong et al., 2010, Popov et al., 2010, Roignant et al., 2010, Salzer et al., 2010, Swaminathan et al., 2010, Wang et al., 2010, Bao and Friedrich, 2009, Christensen et al., 2009.2.28, Classen et al., 2009, Dworkin et al., 2009, Grieder et al., 2009, Gutierrez-Aviño et al., 2009, Jiang et al., 2009, Martinez et al., 2009, Wang and Huang, 2009, Anderson and Kumar, 2008, Brás-Pereira and Casares, 2008, Chattopadhyay et al., 2008, Duong et al., 2008, Wang and Sun, 2008, Wang et al., 2008, Yao et al., 2008, Christensen et al., 2007.10.29, de Navascués and Modolell, 2007, Kankel et al., 2007, Kumar and Datta, 2007, Kumar and Luhur, 2007, Kumar and Salzer, 2007, Luque and Milan, 2007, Salvany et al., 2007, Sato and Tomlinson, 2007, Anderson et al., 2006, Rusten et al., 2006, Brown and Feder, 2005, Moberg et al., 2005, Roederer et al., 2005)
      Secondary FlyBase IDs
      • FBgn0011647
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (196)