-XP is excluded from the calyx of the larval mushroom body, but not from the lobes.
protein is strongly upregulated just posterior to the morphogenetic furrow in third instar eye discs. After this initial strong expression in the preclusters, Fas2
expression is low in distinct ommatidial cluster patterns in the cell membranes of most photoreceptors. Eventually, elevated levels of Fas2
protein are seen just in one of the photoreceptor cells, namely R7.
is used a marker for immature larval photorecetor cells of Bolwig organ.
In stage 12 embryos, Fas2
protein is detected in the primordium of the pars lateralis. In the larva, it labels the axons of the dorsomedial and anteriolateral neurosecretory cells which form the pars intercerebralis and pars lateralis, respectively.
is a marker for neural cell membranes
Fas2 is present in the periactive zone of the presynapse, but does not precisely colocalize with Dap-160, endoA, or dynamin.
In the adult prothorax and neck, Fas2
immunoreactivity is observed in the type II boutons of motorneurons innervating ventral cervical muscle 27 and prothoracic sternal anterior rotator muscle 31, but is undetectable in terminals located along the cuticle of the coxal midline. Fas2
is not detected at type I boutons of adult prothoracic neuromuscular junctions, unlike the pattern observed in larval neuromuscular junctions. Fas2
immunoreactivity is also observed in the trunk and branches of the cervical nerve, and in the glial cells surrounding the cervical nerve.
protein is expressed in the three longitudinal fascicles of the embryonic longitudinal connectives, and in peripheral motor neurons.
In the developing germarium, Fas2
protein is expressed in all follicle cells during early oogenesis. At stage 7, Fas2
protein becomes apically localized in follicle cells, then is lost in migrating border follicle cells, while being localized to the leading edge of polar follicle cells.
is found in the more mature mature, non-core neurons of the pedunculus of the 3rd instar larvae.
Expression in procephalic neuroblasts stage 9-11: tritocerebrum - d1, d2, d6, v1-3; deuterocerebrum - d9, d11, v2, v8
protein is strongly expressed in the mushroom body alpha and beta lobes, and weakly in the mushroom body gamma-lobe and spur.
At stage 12, two neurons in each hemisphere express Fas2
protein, aCC and pCC. At this stage, the pCC growth cone which pioneers the MP1 pathway expresses Fas2
protein. By stage 13, it is expressed in other cells along the MP1 pathway. By stage 14, the MP1 pathway is continuous from segment to segment and Fas2
protein is expressed along its length as well as in some axons of the intersegmental nerve root including aCC. By stage 16, Fas2
protein is expressed in other longitudinal axon pathways. Outside of the CNS, it is expressed in the periphery in several motoneuron growth cones in each hemisegment including the aCC growth cone. In the PNS, it is expressed in some sensory neurons in most segments. It is expressed in patches in the ectoderm that will give rise to the spiracles, in Malpighian tubules, in the part of the hindgut bordering the Malpighian tubules and in ectodermal patches in the head.