FB2021_06, released December 14, 2021
Gene: Dmel\Fas3
Gene: Dmel\Fas3
FasIII, Fasciclin III, Fas III, FasciclinIII, Fas-III
transmembrane - cam - novel - aids in cell adhesion, axon pathfinding and fasciculation ensuring proper muscle innervation
Please see the JBrowse view of Dmel\Fas3 for information on other features
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Gene model reviewed during 5.51
Alternative translation stop created by use of multiphasic reading frames within coding region.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.55
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Fas3 using the Feature Mapper tool.
Expression of Fas3 in wild-type embryos is higher on the inside of the hindgut curve.
Comment: localization restricted from cell membrane directly abutting the amnioserosa
Comment: low level
Fas3 expression is restricted to the inside of the hindgut curve, along the entire lateral margin of the curve.
Fas2 protein concentrates at hub cell junctions.
Fas3 is a marker for hub cells. It is expressed in the anterior region of male gonads from embryonic stage 15 on.
Fas3 is expressed in the anterior cluster of somatic gonadal precursor cells in the stage 17 embryonic gonad. It is also expressed in the adult hub.
Fas3 protein is expressed 4-5 polar follicle cell precursor cells in stage 1-4 egg chambers, and later in the 2 polar cells that emerge from the prepolar group.
Fas3 protein is localized to the basolateral surface of cells of the wing disc.
Fas3 protein is expressed in the visceral mesoderm, and strongly in muscle founder cells.
GBrowse - Visual display of RNA-Seq signals
View Dmel\Fas3 in GBrowse 2
2-53
2-53.3
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
monoclonal antibody
monoclonal
Source for identity of: Fas3 CG5803
Not required for viability.
Fas3 protein mediates synaptic target recognition through homophilic interaction.
Some of the proteins of apico-lateral junctions are required both for apico-basal cell polarity and for the signalling mechanisms controlling cell proliferation, whereas others are required more specifically in cell-cell signalling.
The primary target genes of Egfr are pnt, vnd and Fas3, these are induced in different ectodermal domains. Secondary target genes oc, argos and trn are activated by pnt in response to Egfr signalling. The proper induction of these genes requires the concomitant inactivation of aop, mediated by Egfr signalling.
Regions important for homophilic specificity are defined and the crystal structure determined.
In pros mutants, where innervation of muscles fails, connectin and Fas3 are expressed on muscle surface begins in same developmental pattern as in wild type. However Fas3 expression declines towards the end of embryogenesis, whereas it persists in wild type.
The phenotypes of Fas1, Fas2, Fas3 and nac mutants were analysed in the developing wing: the axon tracts in the CNS for the most part remain unaltered, and none of the phenotypes are 100% penetrant, indicating a fine-tuning role for these molecules in both the PNS and CNS.
Analysis of Fas3 localisation suggest that the protein plays a role in growth cone guidance.
Identified by screening a cDNA expression library with monoclonal antibodies directed against embryonic CNS and shown to identify proteins expressed on the cell surface of a restricted subset of cells of the embryo, including specific cells of the developing central nervous system. Monoclonal-antibody staining reveals a complex sequence of temporal and spatial expression of Fas3. It is expressed on segmentally repeated patches of cells during neurogenesis and outside the developing CNS on segmentally repeated stripes in the epidermis at the segmental grooves, on patches of epithelial cells near the stomodeal and proctodeal invaginations, on the visceral but not the somatic mesoderm, and on the luminal surface of the salivary gland epithelium; expression is highest where fasciclin-III-positive cells contact one another. After germ-band extension transiently expressed on segmentally repeated patches of neuroepithelial cells and specific underlying neuronal lineages; by the end of germ band retraction fasciclin-III is expressed in repeated stripes across all body segments. At hour 12 fasciclin is detected on a subset of three axon bundles (fascicles) in the anterior commissure and two in the posterior commissure of each segment; generally, however it's not expressed on the axons of these neurons that exit CNS, specifically on the longitudinal fascicles. Expression of Fas3 in transfected cultured cells promotes their adhesion to each other, but not to nonexpressing cells (Snow, Bieber and Goodman, 1989). The monoclonal antibodies used in the isolation of fasciclin identify four closely related membrane glycoproteins of 80, 66, 59 and 46kD molecular weight; all depend on the presence of Fas3+ for their presence.
