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Gene Dmel\fj

General Information
Symbol	Dmel\fj	Species	D. melanogaster
Name	four-jointed	Annotation symbol	CG10917
Feature type	protein_coding_gene	FlyBase ID	FBgn0000658
Gene Model Status	Current	Stock availability	14 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map	2-81.5
Cytogenetic map	55C1-55C1	Sequence location	2R:14,120,266..14,123,816 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene four-jointed is referred to in FlyBase by the symbol Dmel\fj (CG10917, FBgn0000658). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: Wnt-protein binding; protein kinase activity. There is experimental evidence that it is involved in the biological process: establishment of planar polarity; establishment of imaginal disc-derived wing hair orientation; cell-cell signaling; imaginal disc-derived wing vein specification; establishment of epithelial cell apical/basal polarity; regulation of tube length, open tracheal system; imaginal disc growth; imaginal disc-derived leg joint morphogenesis; Wnt receptor signaling pathway, planar cell polarity pathway. 51 alleles are reported. The phenotypes of these alleles are annotated with: adult segment; organ system subdivision; organ system; appendage segment; thoracic segment; external compound sense organ; imaginal precursor; peripheral nervous system; embryonic/larval imaginal precursor; tracheal system; integumentary specialisation; metatarsus. It has one annotated transcript and one annotated polypeptide. Protein features are: Four-jointed box protein 1/four-jointed protein. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1636091_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of fj. Gene sequence location is 2R:14120266..14123816.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	fj: four jointed fj: four jointed Edith M. Wallace, unpublished. Similar to d. Tarsi four instead of five jointed. Legs short and stocky, owing to failure of joint formation between second and third tarsal segments (Tokunaga and Gerhart, 1976, Genetics 83: s76). Femur, tibia, and tarsae foreshortened [Tokunaga and Gerhart, 1976; Mikuta, 1979, Genetika (Moscow) 15: 624-32]. Probability of joint failure proportional to degree of shortening of second tarsal segment (Tokunaga and Gerhart, 1976). Joint failure phenotype can extend into fj/+ tissue adjacent to fj/fj clones (Tokunaga and Gerhart, 1976). Leg chaetae show irregularities in the relative orientation of sockets and bracts (Held, Duarte, and Derakhshanian, 1986, Wilhelm Roux's Arch. Dev. Biol. 195: 145-57). Enhanced by ssa and ssaB [Villee, 1945, Genetics 30: 26-27; Mglinetz and Ivanov, 1976, Genetika (Moscow) 12: 87-94] and by pb [Kaurov, Ivanov, and Mglinetz, 1978, Genetika (Moscow) 14: 306-12]; also influenced by AntpNs (Mikuta and Mglinetz). fj eyD flies have but three tarsal joints (Postlethwait and Schneiderman, 1975, Ann. Rev. Genet. 7: 381-433). Development similar to that of dachs [Waddington, 1943, J. Genet. 45: 29-43 (fig.)]. Wings shorter and broader with crossveins conspicuously closer together; veins diverge at greater angle (Tokunaga, Michinomae, Sizemore, and Gerhart, 1978, Genetics 88: s98). Effect visible in pupal wing (Waddington, 1940, J. Genet. 41: 75-139). Eyes smaller, ellipsoid, coarse textured; head foreshortened. RK2.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 55C1-55C1   Limits computationally determined from genome sequence between P{PZ}Hsf03091&P{EP}Dgp-1EP731 and P{PZ}sbb04525&P{EP}EP1081EP1081
Experimentally Determined Cytological Location
Cytogenetic map Notes References 55C-55C     (Brodsky, 1996.1.9) 55B-55C   (determined by in situ hybridisation)   (Villano and Katz, 1995) 55C-55C     (Brodsky and Steller, 1995)
Experimentally Determined Recombination Data
Location	2-81.5
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\fj for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) fj-RA FBtr0086754  NM_058135   3551   583
Additional Transcript Data & Comments
Reported size (kB)	3.7 (northern blot) (Villano and Katz, 1995) 3.5 (northern blot) (Brodsky and Steller, 1996)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein fj-PA   FBpp0085933   65.5   583   8.74     NP_477483   AAF57724
Additional Polypeptide Data & Comments
Reported size (kDa)	583 (aa); 66 (kD predicted) (Villano and Katz, 1995) 583 (aa) (Brodsky and Steller, 1996)
Comments	Rabbit antibody raised against amino acids 485-583. Rat antibody raised against amino acids 111-433. (Strutt et al., 2004)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Four-jointed box protein 1/four-jointed protein (IPR024868)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141599   AE013599 AAF57724   AC004295   AQ073374   AY071147 AAL48769   BH759403   BI171790   BI212842   BI366606   CK659658   CO281550   CO288070   CO295606   CO311424   CO322528   CO332936   CZ478040   EC066454   EC075501   EC088115   EC194720   EC195057   EC213682   EC223532   EC228782   EC242008   EC245317   FF579230   FF579413   GH741315   GH741889   GH742972   GH743324   GH743442   GH744290   GH744513   GH745947   GH746494   GH747192   GH762534   GH764269   GH764640   GH764946   GH765220   U28837 AAA69524   U44904 AAB01809
UniProtKB/Swiss-Prot	P54360
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 4 -- 6   central brain anlage in statu nascendi   (Fisher et al., 2012) dorsal ectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi mesoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi ventral ectoderm anlage   (Fisher et al., 2012) Comment:anlage in statu nascendi yolk nucleus   (Fisher et al., 2012) antennal anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi dorsal head epidermis anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi visual anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi embryonic stage 7 -- 8   amnioserosa anlage   (Fisher et al., 2012) antennal anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage central brain anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal head epidermis anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage visual anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal ectoderm   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) embryonic stage 9 -- 10   amnioserosa   (Fisher et al., 2012) amnioserosa primordium   (Fisher et al., 2012) antennal primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium dorsal ectoderm   (Fisher et al., 2012) foregut primordium   (Fisher et al., 2012) embryonic stage 11 -- 12   amnioserosa   (Fisher et al., 2012) dorsal epidermis primordium   (Fisher et al., 2012) dorsal imaginal precursor   (Fisher et al., 2012) Comment:reported as dorsal imaginal precursor specific anlage embryonic optic lobe primordium   (Fisher et al., 2012) gnathal primordium   (Fisher et al., 2012) hindgut proper primordium   (Fisher et al., 2012) posterior spiracle primordium   (Fisher et al., 2012) Comment:reported as posterior spiracle specific anlage tracheal primordium   (Fisher et al., 2012) ventral apodeme anlage   (Fisher et al., 2012) ventral epidermis primordium   (Fisher et al., 2012) ventral imaginal precursor   (Fisher et al., 2012) Comment:reported as ventral imaginal precursor specific anlage ventral sensory complex anlage   (Fisher et al., 2012) visceral branch primordium   (Fisher et al., 2012) Comment:reported as visceral branch specific anlage embryonic stage 13 -- 16   amnioserosa   (Fisher et al., 2012) atrium   (Fisher et al., 2012) embryonic anal pad   (Fisher et al., 2012) embryonic dorsal epidermis   (Fisher et al., 2012) embryonic epipharynx   (Fisher et al., 2012) embryonic foregut   (Fisher et al., 2012) embryonic head epidermis   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic hypopharynx   (Fisher et al., 2012) embryonic proventriculus intermediate layer   (Fisher et al., 2012) embryonic small intestine   (Fisher et al., 2012) embryonic ventral apodeme   (Fisher et al., 2012) embryonic ventral epidermis   (Fisher et al., 2012) embryonic/larval posterior spiracle   (Fisher et al., 2012) third instar larval stage   eye-antennal disc | gradient   (Brodsky and Steller, 1996) ventral thoracic disc | restricted   (Villano and Katz, 1995) ventral thoracic disc   (Brodsky and Steller, 1996) larval outer optic anlage   (Villano and Katz, 1995) eye-antennal disc | restricted   (Villano and Katz, 1995) lamina   (Brodsky and Steller, 1996) wing disc | restricted   (Villano and Katz, 1995) wing disc   (Brodsky and Steller, 1996) pupal stage   ventral thoracic disc | restricted   (Villano and Katz, 1995) tibia   (Villano and Katz, 1995)
Additional Descriptive Data
	The fj transcript expression pattern closely resembles the enhancer trap staining pattern. Transcripts are expressed in concentric circles in leg discs from third instar larvae. At 4-6hrs after puparium formation, the disc has everted and the future tarsal segment boundaries are visible. A line of positive cells is seen adjacent to each tarsal furrow, with additional strong staining in the first tarsal segment and in the tibia. Weak expression in semicircular arcs is seen in the third instar antennal disc. Expression in the wing disc is concentrated in the prospective wing blade region. In eye discs, the strongest expression is observed in the central portion of the disc just anterior to the morphogenetic furrow, with no expression in the lateral portions of the disc. A gradient of expression is seen declining toward the posterior tip of the disc. In the larval brain, expression is concentrated in the outer optic anlage (Villano and Katz, 1995) fj transcripts are expressed at the highest level near the equator of the wing disc, are lower in intermediate regions, and are lowest near the dorsal and ventral poles. In the larval brain, they are expressed in laminar neuronal precursor cells and are again present in a gradient. In the wing disc, fj transcripts are expressed in a large central region including the wing pouch and in the leg disc, they are found in two concentric circles. (Brodsky and Steller, 1996)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference third instar larval stage   wing pouch   (Strutt et al., 2004) morphogenetic furrow | anterior to & gradient   (Strutt et al., 2004) ventral thoracic disc | restricted   (Strutt et al., 2004) Comment:concentric rings prepupal stage P3   wing pouch   (Strutt et al., 2004) Comment:at 4.5 hr APF metatarsus   (Strutt et al., 2004) Comment:at 4.5 hr APF
Additional Descriptive Data
	fj protein is observed to be localized in a gradient ahead of the morphogenetic furrow in the eye disc, with highest expression near the dorsal-ventral midline. fj protein expression in the leg disc is observed in weak concentric rings, possibly corresponding to segment boundaries. Expression is weak in the pupal leg disc, with a stripe at the presumptive tarsal segment 1. Wing disc expression of fj protein is observed in the wing pouch in larval and pupal discs. (Strutt et al., 2004)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
plasma membrane inferred from direct assay AND inferred from sequence or structural similarity (Villano and Katz, 1995)
Expression Deduced from Reporters
Reporter: P{GawB}fjVG1 Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism | restricted   (Tang and Sun, 2002) third instar larval stage   ventral thoracic disc | restricted   (Tang and Sun, 2002) wing disc | restricted   (Tang and Sun, 2002) eye-antennal disc | restricted   (Tang and Sun, 2002) Reporter: P{lacW}fjp1 Stage Tissue/Position (including subcellular localization) Reference larval stage   embryonic/larval brain   (Brodsky and Steller, 1996) third instar larval stage   eye-antennal disc | gradient   (Brodsky and Steller, 1996) ventral thoracic disc | restricted   (Rauskolb and Irvine, 1999) Reporter: P{lacW}fjp2 Stage Tissue/Position (including subcellular localization) Reference larval stage   embryonic/larval brain   (Brodsky and Steller, 1996) third instar larval stage   eye-antennal disc | gradient   (Brodsky and Steller, 1996) Reporter: P{lacW}fjp3 Stage Tissue/Position (including subcellular localization) Reference larval stage   embryonic/larval brain   (Brodsky and Steller, 1996) third instar larval stage   eye-antennal disc | gradient   (Brodsky and Steller, 1996) Reporter: P{lacW}fjp4 Stage Tissue/Position (including subcellular localization) Reference larval stage   embryonic/larval brain   (Brodsky and Steller, 1996) third instar larval stage   eye-antennal disc | gradient   (Brodsky and Steller, 1996) Reporter: P{lacW}fjp5 Stage Tissue/Position (including subcellular localization) Reference larval stage   embryonic/larval brain   (Brodsky and Steller, 1996) third instar larval stage   eye-antennal disc | gradient   (Brodsky and Steller, 1996) Reporter: P{lacW}fjp6 Stage Tissue/Position (including subcellular localization) Reference larval stage   embryonic/larval brain   (Brodsky and Steller, 1996) third instar larval stage   eye-antennal disc | gradient   (Brodsky and Steller, 1996) Reporter: P{lacW}P55Cg4 Stage Tissue/Position (including subcellular localization) Reference larval stage   embryonic/larval brain   (Brodsky and Steller, 1996) third instar larval stage   eye-antennal disc | gradient   (Brodsky and Steller, 1996) Reporter: P{lacW}P55Ci10 Stage Tissue/Position (including subcellular localization) Reference larval stage   embryonic/larval brain   (Brodsky and Steller, 1996) third instar larval stage   eye-antennal disc | gradient   (Brodsky and Steller, 1996) Reporter: P{lacW}P55Ci20 Stage Tissue/Position (including subcellular localization) Reference larval stage   embryonic/larval brain   (Brodsky and Steller, 1996) third instar larval stage   eye-antennal disc | gradient   (Brodsky and Steller, 1996)
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    56.8 Larval Midgut    2.1 Larval Hindgut    29.7 Larval Malpighian Tubules    2.4 Larval Fat Body    4.8 Larval Salivary Gland    5.1 Larval Trachea    80.525 Larval Carcass    59.55 Adult Head    8.1 Adult Eye    15.45 Adult Brain    3.8 Adult Thoracic-Abdominal Ganglion    2.5 Adult Crop    20.9 Adult Midgut    1 Adult Hindgut    6.9 Adult Malpighian Tubules    3.4 Adult Fat Body    1.3 Adult Salivary Gland    1.4 Adult Heart    1.725 Adult VirginFemale Spermatheca    4.3 Adult InseminatedFemale Spermatheca    3.5 Adult Ovary    0.8 Adult Testis    1.3 Adult Male Accessory Gland    0.9 Adult Carcass    5.4 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    56.8 Larval Midgut    2.1 Larval Hindgut    29.7 Larval Malpighian Tubules    2.4 Larval Fat Body    4.8 Larval Salivary Gland    5.1 Larval Trachea    80.525 Larval Carcass    59.55 Adult Head    8.1 Adult Eye    15.45 Adult Brain    3.8 Adult Thoracic-Abdominal Ganglion    2.5 Adult Crop    20.9 Adult Midgut    1 Adult Hindgut    6.9 Adult Malpighian Tubules    3.4 Adult Fat Body    1.3 Adult Salivary Gland    1.4 Adult Heart    1.725 Adult VirginFemale Spermatheca    4.3 Adult InseminatedFemale Spermatheca    3.5 Adult Ovary    0.8 Adult Testis    1.3 Adult Male Accessory Gland    0.9 Adult Carcass    5.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    56.8 Larval Midgut    2.1 Larval Hindgut    29.7 Larval Malpighian Tubules    2.4 Larval Fat Body    4.8 Larval Salivary Gland    5.1 Larval Trachea    80.525 Larval Carcass    59.55 Adult Head    8.1 Adult Eye    15.45 Adult Brain    3.8 Adult Thoracic-Abdominal Ganglion    2.5 Adult Crop    20.9 Adult Midgut    1 Adult Hindgut    6.9 Adult Malpighian Tubules    3.4 Adult Fat Body    1.3 Adult Salivary Gland    1.4 Adult Heart    1.725 Adult VirginFemale Spermatheca    4.3 Adult InseminatedFemale Spermatheca    3.5 Adult Ovary    0.8 Adult Testis    1.3 Adult Male Accessory Gland    0.9 Adult Carcass    5.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    56.8 Larval Midgut    2.1 Larval Hindgut    29.7 Larval Malpighian Tubules    2.4 Larval Fat Body    4.8 Larval Salivary Gland    5.1 Larval Trachea    80.525 Larval Carcass    59.55 Adult Head    8.1 Adult Eye    15.45 Adult Brain    3.8 Adult Thoracic-Abdominal Ganglion    2.5 Adult Crop    20.9 Adult Midgut    1 Adult Hindgut    6.9 Adult Malpighian Tubules    3.4 Adult Fat Body    1.3 Adult Salivary Gland    1.4 Adult Heart    1.725 Adult VirginFemale Spermatheca    4.3 Adult InseminatedFemale Spermatheca    3.5 Adult Ovary    0.8 Adult Testis    1.3 Adult Male Accessory Gland    0.9 Adult Carcass    5.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    56.8 Larval Midgut    2.1 Larval Hindgut    29.7 Larval Malpighian Tubules    2.4 Larval Fat Body    4.8 Larval Salivary Gland    5.1 Larval Trachea    80.525 Larval Carcass    59.55 Adult Head    8.1 Adult Eye    15.45 Adult Brain    3.8 Adult Thoracic-Abdominal Ganglion    2.5 Adult Crop    20.9 Adult Midgut    1 Adult Hindgut    6.9 Adult Malpighian Tubules    3.4 Adult Fat Body    1.3 Adult Salivary Gland    1.4 Adult Heart    1.725 Adult VirginFemale Spermatheca    4.3 Adult InseminatedFemale Spermatheca    3.5 Adult Ovary    0.8 Adult Testis    1.3 Adult Male Accessory Gland    0.9 Adult Carcass    5.4 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    56.8 Larval Midgut    2.1 Larval Hindgut    29.7 Larval Malpighian Tubules    2.4 Larval Fat Body    4.8 Larval Salivary Gland    5.1 Larval Trachea    80.525 Larval Carcass    59.55 Adult Head    8.1 Adult Eye    15.45 Adult Brain    3.8 Adult Thoracic-Abdominal Ganglion    2.5 Adult Crop    20.9 Adult Midgut    1 Adult Hindgut    6.9 Adult Malpighian Tubules    3.4 Adult Fat Body    1.3 Adult Salivary Gland    1.4 Adult Heart    1.725 Adult VirginFemale Spermatheca    4.3 Adult InseminatedFemale Spermatheca    3.5 Adult Ovary    0.8 Adult Testis    1.3 Adult Male Accessory Gland    0.9 Adult Carcass    5.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    56.8 Larval Midgut    2.1 Larval Hindgut    29.7 Larval Malpighian Tubules    2.4 Larval Fat Body    4.8 Larval Salivary Gland    5.1 Larval Trachea    80.525 Larval Carcass    59.55 Adult Head    8.1 Adult Eye    15.45 Adult Brain    3.8 Adult Thoracic-Abdominal Ganglion    2.5 Adult Crop    20.9 Adult Midgut    1 Adult Hindgut    6.9 Adult Malpighian Tubules    3.4 Adult Fat Body    1.3 Adult Salivary Gland    1.4 Adult Heart    1.725 Adult VirginFemale Spermatheca    4.3 Adult InseminatedFemale Spermatheca    3.5 Adult Ovary    0.8 Adult Testis    1.3 Adult Male Accessory Gland    0.9 Adult Carcass    5.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    56.8 Larval Midgut    2.1 Larval Hindgut    29.7 Larval Malpighian Tubules    2.4 Larval Fat Body    4.8 Larval Salivary Gland    5.1 Larval Trachea    80.525 Larval Carcass    59.55 Adult Head    8.1 Adult Eye    15.45 Adult Brain    3.8 Adult Thoracic-Abdominal Ganglion    2.5 Adult Crop    20.9 Adult Midgut    1 Adult Hindgut    6.9 Adult Malpighian Tubules    3.4 Adult Fat Body    1.3 Adult Salivary Gland    1.4 Adult Heart    1.725 Adult VirginFemale Spermatheca    4.3 Adult InseminatedFemale Spermatheca    3.5 Adult Ovary    0.8 Adult Testis    1.3 Adult Male Accessory Gland    0.9 Adult Carcass    5.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    8.1   NA  Eye    no informative data   NA  Brain    3.8   56.8  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    2.5   NA  Crop    no informative data   2.1  Midgut    1   29.7  Hindgut    6.9   2.4  Malpighian Tubules    3.4   4.8  Fat Body    1.3   5.1  Salivary Gland    1.4   NA  Heart    1.725   80.525  Trachea    NA   NA  VirginFemale Spermatheca    4.3   NA  InseminatedFemale Spermatheca    3.5   NA  Ovary    0.8   NA  Testis    1.3   NA  Male Accessory Gland    0.9   59.55  Carcass    5.4 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    10 central nervous system, pupae P8    3 head, virgin 1-day female    4 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    3 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    3 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    5 fat body, pupae P8    4 carcass, larvae L3 wandering    28 carcass, 1-day adult    2 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (35) central nervous system, larvae L3    10 central nervous system, pupae P8    3 head, virgin 1-day female    4 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    3 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    3 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    5 fat body, pupae P8    4 carcass, larvae L3 wandering  (28) carcass, 1-day adult    2 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    10 central nervous system, pupae P8    3 head, virgin 1-day female    4 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    3 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    3 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    5 fat body, pupae P8    4 carcass, larvae L3 wandering    28 carcass, 1-day adult    2 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    10 central nervous system, pupae P8    3 head, virgin 1-day female    4 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    3 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    3 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    5 fat body, pupae P8    4 carcass, larvae L3 wandering    28 carcass, 1-day adult    2 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    10 central nervous system, pupae P8    3 head, virgin 1-day female    4 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    3 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    3 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    5 fat body, pupae P8    4 carcass, larvae L3 wandering    28 carcass, 1-day adult    2 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (35) central nervous system, larvae L3    10 central nervous system, pupae P8    3 head, virgin 1-day female    4 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    3 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    3 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    5 fat body, pupae P8    4 carcass, larvae L3 wandering  28 carcass, 1-day adult    2 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    10 central nervous system, pupae P8    3 head, virgin 1-day female    4 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    3 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    3 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    5 fat body, pupae P8    4 carcass, larvae L3 wandering    28 carcass, 1-day adult    2 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    10 central nervous system, pupae P8    3 head, virgin 1-day female    4 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    3 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    3 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    5 fat body, pupae P8    4 carcass, larvae L3 wandering    28 carcass, 1-day adult    2 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    19 embryo 04-06hr    52 embryo 06-08hr    83 embryo 08-10hr    69 embryo 10-12hr    49 embryo 12-14hr    35 embryo 14-16hr    16 embryo 16-18hr    13 embryo 18-20hr    12 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    14 white prepupae new    14 white prepupae 12hr    24 white prepupae 24hr    28 pupae 2d postWPP    16 pupae 3d postWPP    10 pupae 4d postWPP    12 adult male 01day    4 adult male 05day    1 adult male 30day    0 adult female 01day    2 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    19 embryo 04-06hr  (52) embryo 06-08hr  (83) embryo 08-10hr  (69) embryo 10-12hr  (49) embryo 12-14hr  (35) embryo 14-16hr    16 embryo 16-18hr    13 embryo 18-20hr    12 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    14 white prepupae new    14 white prepupae 12hr    24 white prepupae 24hr  (28) pupae 2d postWPP    16 pupae 3d postWPP    10 pupae 4d postWPP    12 adult male 01day    4 adult male 05day    1 adult male 30day    0 adult female 01day    2 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    19 embryo 04-06hr    52 embryo 06-08hr    83 embryo 08-10hr    69 embryo 10-12hr    49 embryo 12-14hr    35 embryo 14-16hr    16 embryo 16-18hr    13 embryo 18-20hr    12 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    14 white prepupae new    14 white prepupae 12hr    24 white prepupae 24hr    28 pupae 2d postWPP    16 pupae 3d postWPP    10 pupae 4d postWPP    12 adult male 01day    4 adult male 05day    1 adult male 30day    0 adult female 01day    2 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    19 embryo 04-06hr    52 embryo 06-08hr    83 embryo 08-10hr    69 embryo 10-12hr    49 embryo 12-14hr    35 embryo 14-16hr    16 embryo 16-18hr    13 embryo 18-20hr    12 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    14 white prepupae new    14 white prepupae 12hr    24 white prepupae 24hr    28 pupae 2d postWPP    16 pupae 3d postWPP    10 pupae 4d postWPP    12 adult male 01day    4 adult male 05day    1 adult male 30day    0 adult female 01day    2 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    19 embryo 04-06hr    52 embryo 06-08hr    83 embryo 08-10hr    69 embryo 10-12hr    49 embryo 12-14hr    35 embryo 14-16hr    16 embryo 16-18hr    13 embryo 18-20hr    12 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    14 white prepupae new    14 white prepupae 12hr    24 white prepupae 24hr    28 pupae 2d postWPP    16 pupae 3d postWPP    10 pupae 4d postWPP    12 adult male 01day    4 adult male 05day    1 adult male 30day    0 adult female 01day    2 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    19 embryo 04-06hr  (52) embryo 06-08hr  (83) embryo 08-10hr  (69) embryo 10-12hr  (49) embryo 12-14hr  (35) embryo 14-16hr    16 embryo 16-18hr    13 embryo 18-20hr    12 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    14 white prepupae new    14 white prepupae 12hr    24 white prepupae 24hr  28 pupae 2d postWPP    16 pupae 3d postWPP    10 pupae 4d postWPP    12 adult male 01day    4 adult male 05day    1 adult male 30day    0 adult female 01day    2 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    19 embryo 04-06hr    52 embryo 06-08hr    83 embryo 08-10hr    69 embryo 10-12hr    49 embryo 12-14hr    35 embryo 14-16hr    16 embryo 16-18hr    13 embryo 18-20hr    12 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    14 white prepupae new    14 white prepupae 12hr    24 white prepupae 24hr    28 pupae 2d postWPP    16 pupae 3d postWPP    10 pupae 4d postWPP    12 adult male 01day    4 adult male 05day    1 adult male 30day    0 adult female 01day    2 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    19 embryo 04-06hr    52 embryo 06-08hr    83 embryo 08-10hr    69 embryo 10-12hr    49 embryo 12-14hr    35 embryo 14-16hr    16 embryo 16-18hr    13 embryo 18-20hr    12 embryo 20-22hr    8 embryo 22-24hr    9 larva L1    7 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    14 white prepupae new    14 white prepupae 12hr    24 white prepupae 24hr    28 pupae 2d postWPP    16 pupae 3d postWPP    10 pupae 4d postWPP    12 adult male 01day    4 adult male 05day    1 adult male 30day    0 adult female 01day    2 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    8 ovary OSC    2 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    8 ovary OSC    2 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    8 ovary OSC    2 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    8 ovary OSC    2 ovary OSS    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    8 ovary OSC    2 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    8 ovary OSC    2 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    8 ovary OSC    2 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    8 ovary OSC    2 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    1 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    1 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    1 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    1 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    1 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    1 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    1 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    1 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    1 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_11 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • RE18087 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000658 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10917-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for fj Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal (with fjd3) fjVG1 lethal (with fjVG1) fjd3 lethal | recessive fjVG1 viable fj2 fj3 fj4 fj9-II fjCB04634 fjd1 viable, with Scer\GAL4pnr-MD237 fjGD430 Other Phenotypes Allele cell polarity defective, with Scer\GAL4αTub84B.PL fjScer\UAS.T:SV5\V5 cell polarity defective | cell non-autonomous | somatic clone fjd1 cell polarity defective | cell non-autonomous | somatic clone, with Scer\GAL4Ubx.PdC fjScer\UAS.cZa cell polarity defective | cell non-autonomous | somatic clone, with Scer\GAL4αTub84B.PL fjScer\UAS.cZa cell polarity defective | somatic clone fjN7 planar polarity defective fjd1 planar polarity defective, with Scer\GAL4ap-md544 fjScer\UAS.cZa planar polarity defective, with Scer\GAL4bi-omb3-GAL4 fjScer\UAS.cZa planar polarity defective, with Scer\GAL4bi-omb-Gal4 fj102-583.Scer\UAS.T:Rnor\CD2 fj109-583.Scer\UAS.T:Hsap\GALNT3 fjScer\UAS.cZa fjUn.Scer\UAS planar polarity defective, with Scer\GAL4en-e16E fjScer\UAS.cZa planar polarity defective, with Scer\GAL4ptc-559.1 fjScer\UAS.cZa planar polarity defective, with Scer\GAL4salm-459.2 fjScer\UAS.cZa planar polarity defective, with Scer\GAL4Scer\FRT.Act5C fjScer\UAS.cZa planar polarity defective (with Df(2R)Pcl11B) fjd1 planar polarity defective | cell autonomous | somatic clone fjd1 planar polarity defective | cell non-autonomous | somatic clone fjd1 planar polarity defective | cell non-autonomous | somatic clone, with fjd1 fjt14.T:Hsap\GALNT3 planar polarity defective | cell non-autonomous | somatic clone, with fjt14.T:Hsap\GALNT3 fjd1 planar polarity defective | cell non-autonomous | somatic clone, with Scer\GAL4Act5C.PI fj102-583.Scer\UAS.T:Rnor\CD2 fj109-583.Scer\UAS.T:Hsap\GALNT3 fjScer\UAS.cZa planar polarity defective | cell non-autonomous | somatic clone, with Scer\GAL4αTub84B.PL fjScer\UAS.cZa planar polarity defective | recessive fjd1 planar polarity defective | somatic clone, with fjd1 fjt14.T:Hsap\GALNT3 planar polarity defective | somatic clone, with fjt14.T:Hsap\GALNT3 fjd1 visible fj2 fj3 fj4 fj40e fjhs.PB fjN7 fjp1 visible, with Scer\GAL469B fjScer\UAS.cZa visible, with Scer\GAL4dpp.blk1 fjScer\UAS.cZa visible, with Scer\GAL4en-e16E fjScer\UAS.cZa visible, with Scer\GAL4ptc-559.1 fjScer\UAS.cZa visible | dominant fjVG1 visible | recessive fj1 fjd1 fjd2 fjp1 fjp2 fjp3 fjp4 fjp5 fjp6 visible | somatic clone, with Scer\GAL4Act5C.PI fjScer\UAS.cZa Phenotype manifest in Allele abdomen fjp1 abdominal segment 2 & macrochaeta fjd1 abdominal segment 2 & microchaeta fjd1 abdominal tergite 2 fjunspecified adult cuticle | cell non-autonomous | somatic clone fjd1 adult cuticle | cell non-autonomous | somatic clone, with Scer\GAL4Ubx.PdC fjScer\UAS.cZa adult cuticle | cell non-autonomous | somatic clone, with Scer\GAL4αTub84B.PL fjScer\UAS.cZa anterior crossvein, with Scer\GAL4ptc-559.1 fjScer\UAS.cZa anterior crossvein, with Scer\GAL4Scer\FRT.Act5C fjScer\UAS.cZa arista fjunspecified coxa fjunspecified crossvein fjt14.T:Hsap\GALNT3 crossvein, with Scer\GAL4bi-omb-Gal4 fj102-583.Scer\UAS.T:Rnor\CD2 fj109-583.Scer\UAS.T:Hsap\GALNT3 fjScer\UAS.cZa fjUn.Scer\UAS embryonic/larval dorsal trunk fjd1 eye fjd1 fjd2 fjhs.PB fjp1 eye, with Scer\GAL4bi-omb-Gal4 fjScer\UAS.cZa eye, with Scer\GAL4Scer\FRT.Act5C fjScer\UAS.cZa eye (with Df(2R)Pcl11B) fjd1 eye | dorsal, with Scer\GAL4bi-omb-Gal4 fj102-583.Scer\UAS.T:Rnor\CD2 fj109-583.Scer\UAS.T:Hsap\GALNT3 fjScer\UAS.cZa fjUn.Scer\UAS eye | somatic clone fjd1 eye | ventral, with Scer\GAL4bi-omb-Gal4 fj102-583.Scer\UAS.T:Rnor\CD2 fj109-583.Scer\UAS.T:Hsap\GALNT3 fjScer\UAS.cZa fjUn.Scer\UAS eye disc, with Scer\GAL4bi-omb-Gal4 fjScer\UAS.cZa femur fj2 fj3 joint fj2 fj4 fjN7 joint, with Scer\GAL469B fjScer\UAS.cZa joint | ectopic fjhs.PB joint | ectopic, with Scer\GAL469B fjScer\UAS.cZa leg fj1 fjd1 fjd2 fjhs.PB fjp1 fjp2 fjp3 fjp4 fjp5 fjp6 fjunspecified leg, with Scer\GAL4dpp.blk1 fjScer\UAS.cZa leg, with Scer\GAL4en-e16E fjScer\UAS.cZa leg, with Scer\GAL4Scer\FRT.Act5C fjScer\UAS.cZa leg, with Scer\GAL4αTub84B.PL fjScer\UAS.T:SV5\V5 leg | distal, with Scer\GAL4Scer\FRT.Act5C fjScer\UAS.cZa lens fj2 fj3 macrochaeta | cell non-autonomous | somatic clone fjd1 macrochaeta | cell non-autonomous | somatic clone, with Scer\GAL4Ubx.PdC fjScer\UAS.cZa macrochaeta | cell non-autonomous | somatic clone, with Scer\GAL4αTub84B.PL fjScer\UAS.cZa mesothoracic tergum fjVG1 metatarsus fj2 fj3 fj4 fjd1 microchaeta | cell non-autonomous | somatic clone fjd1 microchaeta | cell non-autonomous | somatic clone, with Scer\GAL4Ubx.PdC fjScer\UAS.cZa microchaeta | cell non-autonomous | somatic clone, with Scer\GAL4αTub84B.PL fjScer\UAS.cZa ommatidium fj2 fj3 ommatidium, with Scer\GAL4bi-omb3-GAL4 fjScer\UAS.cZa ommatidium, with Scer\GAL4bi-omb-Gal4 fj102-583.Scer\UAS.T:Rnor\CD2 fj109-583.Scer\UAS.T:Hsap\GALNT3 fjScer\UAS.cZa fjUn.Scer\UAS ommatidium, with Scer\GAL4Scer\FRT.Act5C fjScer\UAS.cZa ommatidium (with Df(2R)Pcl11B) fjd1 ommatidium | cell non-autonomous | somatic clone, with fjd1 fjt14.T:Hsap\GALNT3 ommatidium | cell non-autonomous | somatic clone, with fjt14.T:Hsap\GALNT3 fjd1 ommatidium | cell non-autonomous | somatic clone, with Scer\GAL4Act5C.PI fj102-583.Scer\UAS.T:Rnor\CD2 fj109-583.Scer\UAS.T:Hsap\GALNT3 fjScer\UAS.cZa ommatidium | somatic clone fjN7 fjd1 ommatidium | somatic clone, with fjd1 fjt14.T:Hsap\GALNT3 ommatidium | somatic clone, with fjt14.T:Hsap\GALNT3 fjd1 photoreceptor cell R3 | somatic clone fjN7 photoreceptor cell R4 | ectopic | somatic clone fjN7 posterior crossvein, with Scer\GAL4Scer\FRT.Act5C fjScer\UAS.cZa scutellum fjunspecified tarsal segment fj1 fjd2 fjp1 tarsal segment 2 fj2 fj3 fj4 fjN7 fjd1 fjhs.PB fjp5 fjp6 fjunspecified tarsal segment 2, with Scer\GAL469B fjScer\UAS.cZa tarsal segment 3 fj2 fj3 fj4 fjN7 fjd1 fjhs.PB fjp5 fjp6 fjunspecified tarsal segment 3, with Scer\GAL469B fjScer\UAS.cZa tarsal segment 4 fjhs.PB tarsal segment, with Scer\GAL469B fjScer\UAS.cZa tarsal segment, with Scer\GAL4dpp.blk1 fjScer\UAS.cZa tarsal segment, with Scer\GAL4ptc-559.1 fjScer\UAS.cZa tarsal segment, with Scer\GAL4Scer\FRT.Act5C fjScer\UAS.cZa tarsal segment (with fjd3) fjVG1 tarsal segment (with fjVG1) fjd3 tarsal segment | somatic clone, with Scer\GAL4Act5C.PI fjScer\UAS.cZa tergite | cell non-autonomous | somatic clone fjd1 tergite | cell non-autonomous | somatic clone, with Scer\GAL4Ubx.PdC fjScer\UAS.cZa tergite | cell non-autonomous | somatic clone, with Scer\GAL4αTub84B.PL fjScer\UAS.cZa third segment of antenna fjunspecified tibia fj2 fj3 trichome & tergite | cell non-autonomous | somatic clone fjd1 trichome & tergite | cell non-autonomous | somatic clone, with Scer\GAL4αTub84B.PL fjScer\UAS.cZa wing fj2 fj3 fj4 fjd1 fjd2 fjhs.PB fjp1 fjt14.T:Hsap\GALNT3 fjunspecified wing, with Scer\GAL4bi-omb-Gal4 fj102-583.Scer\UAS.T:Rnor\CD2 fj109-583.Scer\UAS.T:Hsap\GALNT3 fjScer\UAS.cZa fjUn.Scer\UAS wing, with Scer\GAL4αTub84B.PL fjScer\UAS.T:SV5\V5 wing | anterior/posterior compartment boundary, with Scer\GAL4Scer\FRT.Act5C fjScer\UAS.cZa wing | distal fjd1 wing | distal, with Scer\GAL4Scer\FRT.Act5C fjScer\UAS.cZa wing blade fjd1 wing hair fjd1 wing hair, with Scer\GAL4ap-md544 fjScer\UAS.cZa wing hair, with Scer\GAL4ptc-559.1 fjScer\UAS.cZa wing hair, with Scer\GAL4salm-459.2 fjScer\UAS.cZa wing hair, with Scer\GAL4Scer\FRT.Act5C fjScer\UAS.cZa wing hair | cell autonomous | somatic clone fjd1 wing hair | cell non-autonomous, with Scer\GAL4ptc-559.1 fjScer\UAS.cZa wing hair | cell non-autonomous | somatic clone fjd1 wing hair | posterior compartment, with Scer\GAL4en-e16E fjScer\UAS.cZa wing vein fj40e wing vein, with Scer\GAL4Scer\FRT.Act5C fjScer\UAS.cZa wing vein | cell autonomous | somatic clone fjd1 wing vein | cell non-autonomous | ectopic | somatic clone fjd1 wing vein | cell non-autonomous | somatic clone fjd1 wing vein | ectopic fjp1 wing vein L4, with Scer\GAL4Scer\FRT.Act5C fjScer\UAS.cZa
Classical Alleles ( 23 )
For All Classical Alleles Show
Allele of fj Class Mutagen Stocks Known lesion fj1 spontaneous 5 -- fj4 hypomorphic allele - genetic evidence Delta2-3 1 Yes fj9-II P-element activity 1 Yes fjd1 loss of function allele Delta2-3 1 Yes fje00145 piggyBac transposase 1 -- fjKG03419 P-element activity 1 -- fjN7 hypomorphic allele - genetic evidence P-element activity 1 Yes fj2 amorphic allele - genetic evidence Delta2-3 0 Yes fj333a P-element activity 0 -- fj3 hypomorphic allele - genetic evidence Delta2-3 0 Yes fj40e 0 -- fjCB04634 P-element activity 0 Yes fjd2 Delta2-3 0 Yes fjd3 Delta2-3 0 Yes fjEP2183 P-element activity 0 -- fjp1 Delta2-3 0 Yes fjp2 Delta2-3 0 Yes fjp3 Delta2-3 0 Yes fjp4 Delta2-3 0 Yes fjp5 Delta2-3 0 Yes fjp6 Delta2-3 0 Yes fjunspecified 0 -- fjVG1 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 28 )
For All Alleles Carried on Transgenic Constructs Show
Allele of fj Class Mutagen Stocks Known lesion fjGD430 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes fjHMS01310 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes fjJF02843 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes fj+t14 in vitro construct - genomic fragment 0 Yes fj+t24 in vitro construct - genomic fragment 0 Yes fj102-583.MtnA.T:Rnor\CD2 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes fj102-583.Scer\UAS.T:Rnor\CD2 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes fj109-583.MtnA.T:Hsap\GALNT3,T:Zzzz\FLAG in vitro construct - coding region fusion 0 Yes fj109-583.MtnA.T:Hsap\GALNT3 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes fj109-583.Scer\UAS.T:Hsap\GALNT3,T:Zzzz\FLAG in vitro construct - coding region fusion 0 Yes fj109-583.Scer\UAS.T:Hsap\GALNT3 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes fjD447Q.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes fjD454Q.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes fjD490Q.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes fjecd-1.Scer\UAS in vitro construct - regulatory fusion 0 Yes fjecd.Scer\UAS in vitro construct - regulatory fusion 0 Yes fjGGG.Scer\UAS.T:SV5\V5 in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis in vitro construct - coding region fusion 0 Yes fjhs.PB in vitro construct - regulatory fusion 0 Yes fjScer\UAS.cZa in vitro construct - regulatory fusion 0 Yes fjScer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes fjScer\UAS.T:SV5\V5 in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis in vitro construct - coding region fusion 0 Yes fjt14.T:Hsap\GALNT3 in vitro construct 0 Yes fjt14.T:Rnor\CD2 in vitro construct 0 Yes fjUn.MtnA.T:Zzzz\FLAG in vitro construct - coding region fusion 0 Yes fjUn.MtnA in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes fjUn.Scer\UAS.T:Zzzz\FLAG in vitro construct - coding region fusion 0 Yes fjUn.Scer\UAS in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes fjUn.t14 in vitro construct 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)BSC334 (Christensen et al., 2007.10.29) Df(2R)PC4 (Zeidler et al., 1999, Deng and Rizki, 1988, Villano and Katz, 1995, Brodsky and Steller, 1996) Df(2R)Pcl11B (Zeidler et al., 1999, Deng and Rizki, 1988) Df(2R)Pcl-W5 (Deng and Rizki, 1988)
Not disrupted in	In(2R)Pcl11 (Doane, 2000)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD430} NA P{TRiP.HMS01310} NA P{TRiP.JF02843} NA P{UAS-fj::CD2} NA P{UAS-fj.109-583::GNT} NA P{UAS-fj.109-583.GNT.FLAG} NA P{UAS-fj.ecd-1} NA P{UAS-fj.ecd} NA P{UAS-fj.GFP} NA P{UAS-fj.Un::FLAG} NA P{UAS-fj.Un} NA P{UAS-fj} NA P{UAS-fj-V5.GGG} NA P{UAS-fj-V5} NA reporter construct P{fj.14-lacZ} No heat-shock construct P{hsp70-fj.B} NA characterization construct P{fj.+t14} NA P{fj.+t24} NA P{fj.t14::CD2} NA P{fj.t14::GNT} NA P{fj.Un.t14} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}fjEP2183 NA insertion of enhancer trap P{lacW}fj9-II No P{lacW}fjp1 No P{lacW}fjp2 No P{lacW}fjp3 No P{lacW}fjp4 No P{lacW}fjp5 No P{lacW}fjp6 No P{lacW}l(2)SH2292SH2292 No P{MTW}fj333a No miscellaneous insertions P{?lacW}fjN7 NA P{PTT-GB}fjCB04634 NA P{SUPor-P}fjKG03419 NA PBac{RB}fje00145 NA insertion of enhancer trap binary system P{GawB}fjVG1 No
Gene Ontology: Function, Process & Cellular Component ( 16 unique terms )
Terms Based on Experimental Evidence ( 12 terms )
Molecular Function
CV term Evidence References
protein kinase activity inferred from direct assay (Ishikawa et al., 2008) Wnt-protein binding inferred from physical interaction with Wnt4 (Lim et al., 2005)
Biological Process
CV term Evidence References
cell-cell signaling inferred from direct assay AND inferred from sequence or structural similarity (Villano and Katz, 1995) inferred from expression pattern AND inferred from mutant phenotype (Brodsky and Steller, 1996) establishment of epithelial cell apical/basal polarity inferred from mutant phenotype (Ma et al., 2003) establishment of imaginal disc-derived wing hair orientation inferred from genetic interaction with Wnt4 (Lim et al., 2005) inferred from mutant phenotype (Hogan et al., 2011) establishment of planar polarity inferred from mutant phenotype (Bosveld et al., 2012) imaginal disc-derived leg joint morphogenesis inferred from mutant phenotype (Buckles et al., 2001) imaginal disc-derived wing vein specification inferred from mutant phenotype (Zeidler et al., 2000) imaginal disc growth inferred from mutant phenotype (Villano and Katz, 1995) regulation of tube length, open tracheal system inferred from mutant phenotype (Chung et al., 2009) Wnt receptor signaling pathway, planar cell polarity pathway inferred from genetic interaction with Wnt4 (Lim et al., 2005)
Cellular Component
CV term Evidence References
plasma membrane inferred from direct assay AND inferred from sequence or structural similarity (Villano and Katz, 1995)
Terms Based on Predictions or Assertions ( 7 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cell-cell signaling non-traceable author statement (Swiss-Prot Project Members, 1996.10.1) establishment of ommatidial planar polarity traceable author statement (Strutt and Strutt, 2002, Tree et al., 2002) establishment of planar polarity traceable author statement (Mlodzik, 2002) imaginal disc development non-traceable author statement (Swiss-Prot Project Members, 1996.10.1) proximal/distal pattern formation, imaginal disc non-traceable author statement (Swiss-Prot Project Members, 1996.10.1)
Cellular Component
CV term Evidence References
integral to membrane non-traceable author statement (Swiss-Prot Project Members, 1996.10.1) plasma membrane inferred from sequence or structural similarity (Brodsky and Steller, 1996) non-traceable author statement (Villano, 1995.6.8)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
fj - CG10077 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) fj - CG4611 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) fj - CG6701 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) fj - CG8412 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) fj - Chc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) fj - KdelR anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) fj - Mtpα anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) fj - Psa anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) fj - sec63 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions fj allele Gene References fj4 d (Buckles et al., 2001) fj333a ph-p (Faucheux et al., 2001) fjd1 ds (Strutt et al., 2004, Simon, 2004) ft (Strutt et al., 2004, Matakatsu and Blair, 2004) fjN7 d (Buckles et al., 2001) ds (Yang et al., 2002, Simon, 2004) ena (Buckles et al., 2001) Ser (Buckles et al., 2001) fjt14.T:Hsap\GALNT3 ds (Strutt et al., 2004) ft (Strutt et al., 2004) fjunspecified cg (Waddington, 1953) unspecified Abl (Buckles et al., 2001) rho (Sturtevant and Bier, 1995)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62770 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0000658 DroID - A comprehensive database of gene and protein interactions. • FBgn0000658 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37089
Orthologs
OrthoDB Orthologs (29) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG62Z590 Dipteran inclusive ortholog search EOG6C881V Insect inclusive ortholog search EOG65QG99 Arthropod inclusive ortholog search EOG60ZPDM Metazoa inclusive ortholog search EOG6G7QRS
Orthologs in Drosophila Species (EOG62Z590)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\fj     Drosophila simulans   Dsim\GD23059 Y   Drosophila sechellia   Dsec\GM21858 Y   Drosophila erecta   Dere\GG21859 Y   Drosophila yakuba   Dyak\GE11939 Y   Drosophila ananassae   Dana\GF12147 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA10641 Y   Drosophila persimilis   Dper\GL17757 Y   Drosophila willistoni   Dwil\GK22970 Y   Drosophila virilis   Dvir\GJ19878 Y   Drosophila mojavensis   Dmoj\GI20109 Y   Drosophila grimshawi   Dgri\GH21655 Y
Orthologs in non-Drosophila Dipterans (EOG6C881V)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL010745   Anopheles gambiae Malaria mosquito  Agam\AGAP008093   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ801666
Orthologs in non-Dipteran Insects (EOG65QG99)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Bombyx mori Silkmoth  Bmor\BGIBMGA011114   Pediculus humanus Human body louse  Phum\PHUM596540   Tribolium castaneum Red flour beetle  Tcas\GLEAN_15492
Orthologs in non-Insect Arthropods (EOG60ZPDM)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_209341   Daphnia pulex Water flea  Dpux\C21orf62   Daphnia pulex Water flea  Dpux\Fdft1   Ixodes scapularis Deer tick  Isca\ISCW017722
Orthologs in non-Arthropod Metazoa (EOG6G7QRS)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009250gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_018848gn   Danio rerio Zebrafish  Drer\ENSDARG00000035873   Xenopus tropicalis Western clawed frog  Xtro\fjx1   Mus musculus House mouse  Mmus\Fjx1   Rattus norvegicus Norway rat  Rnor\Fjx1   Homo sapiens Human  Hsap\FJX1
Human Orthologs (1)
Gene OMIM HGNC Hsap\FJX1
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD23059 Drosophila sechellia Dsec\GM21858 Drosophila erecta Dere\GG21859 Drosophila yakuba Dyak\GE11939 Drosophila ananassae Dana\GF12147 Drosophila pseudoobscura pseudoobscura Dpse\GA10641 Drosophila persimilis Dper\GL17757 Drosophila willistoni Dwil\GK22970 Drosophila virilis Dvir\GJ19878 Drosophila mojavensis Dmoj\GI20109 Drosophila grimshawi Dgri\GH21655
Stocks & Reagents
Stocks Listed in FlyBase ( 14 )
Bloomington	6371 w1118; fj4 298 fj1 wt1/SM5 6373 w1118; fjd1/CyO 1036 Bc1 fj1 wt1 6391 w*; fjN7/CyO, P{sevRas1.V12}FK1 13782 y1 w67c23; P{SUPor-P}fjKG03419 34323 y1 sc* v1; P{TRiP.HMS01310}attP2
Harvard	- PBac{RB}fje00145
Kyoto	105844 fj1 wt1/SM5 105843 fj1 l(2)Su(H)1/SM5 106179 Bc1 fj1 wt1
VDRC	v6774 w1118; P{GD430}v6774/TM3
	More stocks available...
Genomic Clones ( 2 )
	BACR14N05 BACR15G20 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 39 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE18087
Other clones	DPiM_RE18087
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LP17570 RE13641 RE52180
Other clones	42692 62778 77668 102877 107332 160381 Cauquenes_24_C10 CK01.2C.F8 CK01.13D.E3 CK01.32C.D7 CK01.85C.F10 CK02269 EK067655 EK157427 EK188321 EK210931 EK224527 EK305204 Elena_26_E03 RP001004527 RP001012040 RP001012613 RP001014313 RP001015496 RP001017513 RP001017517 RP001026165 RP001034828 RP001037984 RP001047230 RP001047841 RP001049300 RP001052787 RP001053924
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0000658 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37089
Antibody Information
	polyclonal antibody (Strutt et al., 2004)
Other Information
Discoverer
	Schultz, 1st April 1931.
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Mutation of the DNE motif in fj abolishes its biological activity. (Ishikawa et al., 2008) fj regulates ft signaling by phosphorylating cadherin domains of ft and ds as they transit through the golgi. (Ishikawa et al., 2008) Gradients of fj and ds expression appear to provide partially redundant positional information essential for specifying the polarisation axis in the eye. The fidelity of planar cell polarity in the eye appears to result from the combined action of the fj and ds gradients acting through ft. (Simon, 2004) In the wing, as well as in the eye, fj, ds and ft orient the direction of the fz-mediated intercellular feedback loop. (Ma et al., 2003) fj play an important role in the regulation planar polarity in the compound eye. (Yang et al., 2002) fj can direct ectopic growth and joint initiation in the leg when its normal pattern of expression is disturbed. (Buckles et al., 2001) fj has roles in anterior posterior growth of the abdominal segments and patterning of the wing veins. (Zeidler et al., 2000) fj appears to act in ommatidial polarity as a second signal downstream of wg,hop and wg. (Zeidler et al., 1999) The fj gene was identified in a screen for genes expressed in a non-uniform pattern along the dorsal-ventral axis of the eye. fj is required for proper leg, wing and eye development. Analysis of mutant phenotypes and expression patterns suggests that fj is required for cell-cell signalling during disc development. (Brodsky and Steller, 1996) fj plays a role in a polar coordinate system of positional values in leg tissue. (Brodsky and Steller, 1995) Analysis of the fj phenotype, expression pattern, molecular identity and biochemistry strongly supports previous work that suggests fj functions as a regional signalling molecule regulating growth and differentiation in specific portions of the leg and wing proximal distal axis. An in vitro transcription-translation-coupled translocation assay indicates that fj encodes a type II membrane glycoprotein and that in this in vitro system fj is synthesised in both a membrane bound and a secreted form. (Villano and Katz, 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_058135 RefSeq (Proteins) • NP_477483 DDBJ / EMBL / GenBank • AAA69524 AAB01809 AAF57724 AAL48769 AC004295 AQ073374 AY071147 BH759403 BI212842 CZ478040 U28837 U44904 Entrez Gene - A searchable database of RefSeq genes. • 37089 UniProtKB/Swiss-Prot • P54360
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Four-jointed box protein 1/four-jointed protein (IPR024868) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE18087
Linkouts
	BioGRID - A database of protein and genetic interactions • 62770 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0000658 DroID - A comprehensive database of gene and protein interactions. • FBgn0000658 DRSC - Results from RNAi screens. • FBgn0000658 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000658 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10917-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0000658 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37089 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /dbzhnsky/fourjt1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37089 modMine - Data generated by the modENCODE project. • FBgn0000658
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	CG10917 (Perkins et al., 2009, Ni et al., 2010.12.1) fj (Lawrence et al., 2007, Strutt et al., 2004, Tyler et al., 2007, Strutt and Strutt, 2005, Richter et al., 2011, Tsai and Sun, 2004, Arbouzova and McNeill, 2007, Matakatsu and Blair, 2012, Christensen et al., 2007.10.29, Buszczak et al., 2007, Casal et al., 2006, Lim et al., 2005, Fanto and McNeill, 2004, Slattery et al., 2011, Frise et al., 2010, Fanto et al., 2003, Rogulja et al., 2008, Terriente et al., 2008, Bosveld et al., 2012, Aerts et al., 2009, Feng and Irvine, 2009, Willecke et al., 2008, Sopko et al., 2009, Cho et al., 2006, Willecke et al., 2006, Chung et al., 2009, Grzeschik et al., 2010, Brittle et al., 2010, Shao et al., 2010, Li et al., 2010, Zecca and Struhl, 2010, Herz et al., 2010, Reddy et al., 2010, Beltran et al., 2007, Hogan et al., 2011, Boggiano et al., 2011, Brittle et al., 2012, Bosveld et al., 2012) Fj (Brittle et al., 2007, Simon et al., 2010)
Name Synonym	four-jointed (Lawrence et al., 2007, Strutt and Strutt, 2005, Ishikawa et al., 2008, Le Garrec and Kerszberg, 2008, Rogulja et al., 2008, Feng and Irvine, 2009, Gutierrez-Aviño et al., 2009, Grzeschik et al., 2010, Brittle et al., 2010, Li et al., 2010, Zecca and Struhl, 2010, Hogan et al., 2011, Boggiano et al., 2011) Four-jointed (Strutt, 2005, Mao et al., 2006, Brittle et al., 2007, Collier et al., 2008, Lim et al., 2005, Kerszberg and Wolpert, 2007, Ishikawa et al., 2008, Bosveld et al., 2012, Cho et al., 2006, Reddy et al., 2010) Four-Jointed (Fanto and McNeill, 2004, Bosveld et al., 2012, Neto-Silva et al., 2010) four jointed (Willecke et al., 2006, Shao et al., 2010)
Secondary FlyBase IDs
References ( 143 )
Generate a list of	All references relating to this gene ( 143 )
List References by type	Research paper  ( 73 ) Supplementary material  ( 6 ) Review  ( 22 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 21 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 2 ) Protein sequence record  ( 1 ) Website  ( 1 ) Book  ( 2 ) Curated genome annotation  ( 2 ) Conference report  ( 1 )
Recent research papers ( 11 )
	Ambegaonkar et al., 2012, Curr. Biol. 22(14): 1302--1308 Propagation of Dachsous-Fat planar cell polarity. [FBrf0220288] Bosveld et al., 2012, Science 336(6082): 724--727 Mechanical control of morphogenesis by Fat/Dachsous/Four-jointed planar cell polarity pathway. [FBrf0218281] Brittle et al., 2012, Curr. Biol. 22(10): 907--914 Planar polarity specification through asymmetric subcellular localization of Fat and Dachsous. [FBrf0219618] Matakatsu and Blair, 2012, Development 139(8): 1498--1508 Separating planar cell polarity and Hippo pathway activities of the protocadherins Fat and Dachsous. [FBrf0217773] Rodrigues et al., 2012, Development 139(21): 4051--4061 Activated STAT regulates growth and induces competitive interactions independently of Myc, Yorkie, Wingless and ribosome biogenesis. [FBrf0219660] Boggiano et al., 2011, Dev. Cell 21(5): 888--895 Tao-1 phosphorylates Hippo/MST kinases to regulate the Hippo-Salvador-Warts tumor suppressor pathway. [FBrf0217346] Donoughe and Dinardo, 2011, Development 138(13): 2751--2759 dachsous and frizzled contribute separately to planar polarity in the Drosophila ventral epidermis. [FBrf0213896] Hogan et al., 2011, PLoS Genet. 7(2): e1001305 Two frizzled planar cell polarity signals in the Drosophila wing are differentially organized by the fat/dachsous pathway. [FBrf0213159] Richter et al., 2011, Nat. Cell Biol. 13(9): 1029--1039 The tumour suppressor L(3)mbt inhibits neuroepithelial proliferation and acts on insulator elements. [FBrf0215050] Schwank et al., 2011, Dev. Cell 20(1): 123--130 Antagonistic growth regulation by dpp and fat drives uniform cell proliferation. [FBrf0212765] Slattery et al., 2011, PLoS ONE 6(4): e14686 Genome-wide tissue-specific occupancy of the hox protein ultrabithorax and hox cofactor homothorax in Drosophila. [FBrf0213426] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...