l(1)114, 114, l114, M67
secreted - novel protein - regulates gastrulation via GPCRs - necessary for coordination of cell shape changes throughout the invaginating primordia - regulates motor axon guidance, glial organization and morphogenesis
Gene model reviewed during 5.53
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
Gene model reviewed during 5.56
3.7 (longest cDNA)
May be highly O-glycosylated in its Ser/Thr-rich C-terminal part.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\fog using the Feature Mapper tool.
The pattern of fog transcription precisely precedes the pattern of apical constrictions in the ventral furrow and in the posterior midgut primordia. Zygotic fog transcription begins in the ventral furrow primordium during the beginning of cellularization, about 30 min. before the start of constrictions. The region of fog transcription encompasses a 12-14 cell wide region of the mesoderm primordia. This corresponds to the subset of ventral furrow cells that make up the initial invagination. During invagination, fog transcripts disappear rapidly from the furrow. fog is also expressed in the invagination primordia of the posterior midgut before invagination begins and disappear as the invagination closes.
GBrowse - Visual display of RNA-Seq signalsView Dmel\fog in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: fog CR41108 CR41109 CR41110
fog signal is both necessary and sufficient to trigger the relocalisation of myosin to the apical side of the cell.
New annotation (CR41108) in release 3.2 of the genome annotation. New annotation (CR41109) in release 3.2 of the genome annotation. New annotation (CR41110) in release 3.2 of the genome annotation.
Activation of the fog/cta pathway results in ectopic cell shape changes in the gastrula. The normal location of the ventral furrow in embryos with uniformly expressed fog suggests the existence of a fog-independent pathway determining mesoderm-specific cell behaviours and invagination. Epistasis experiments indicate this pathway requires sna but not twi expression.
The fog gene product is required during gastrulation for two morphogenetic movements, formation of the ventral furrow and invagination of the posterior midgut primordium, and coordinates cell shape changes by inducing apical constriction of cells in a spatially and temporally defined manner. The maternal contribution of fog product can supplement but not substitute for the zygotic activity. Over-expression of fog can induce ectopic constrictions, and hasten the onset of posterior midgut invagination. fog seems to function as a secreted signal that activates the G protein α subunit encoded by cta in neighboring cells.
In fog mutant embryos the early constricting cells in the ventral furrow are scattered throughout most of the mesoderm primordium. As a result of the reduced number of appropriate cell shape changes the ventral furrow invaginates later than wild type and with a much less regular appearance.
Muscle phenotype of mutants studied using polarised light microscopy and antibody staining to detect Mhc-lacZ reporter gene expression in muscles.
Hemizygous lethal; the cellular blastoderm apparently normal and gastrulation begins at normal time. No posterior midgut formed; germ band does not elongate onto dorsal side of embryo but it is thrown into a series of transverse ventral folds. Older embryos often twist around the longitudinal axis for one complete turn; many exhibit an anterodorsal hole through which the brain protrudes and a split in the posterior CNS and protrusion of the midgut. Hemizygous deficiency gives same phenotype. No effect of maternal genotype; homozygous germ line clones make eggs capable of supporting normal embryonic development. Fate mapping localizes fog lethal focus to the posterior pole most likely in presumptive posterior-midgut or proctodaeum cells.
Zygotically active locus involved in the terminal developmental program in the embryo.
Mutations in fog block the transition from the first slow stochastic phase of gastrulation to the second rapid phase of constriction in the ventral furrow and the posterior midgut invaginations.