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General Information
Symbol
Dmel\fs(1)K10
Species
D. melanogaster
Name
female sterile (1) K10
Annotation Symbol
CG3218
Feature Type
FlyBase ID
FBgn0000810
Gene Model Status
Stock Availability
Gene Snapshot
female sterile (1) K10 (fs(1)K10) encodes a nuclear protein of unknown biochemical function required during oogenesis for the proper localization of grk mRNA and protein in the oocyte, a key step in the specification of the dorsoventral axis of the oocyte, mature egg, and future embryo. [Date last reviewed: 2019-09-26]
Also Known As

K10, fs(1)M9, EG:30B8.5

Key Links
Genomic Location
Cytogenetic map
Sequence location
X:2,242,138..2,246,041 [+]
Recombination map

1-0.9

RefSeq locus
NC_004354 REGION:2242138..2246041
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (9 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables DNA binding
inferred from sequence or structural similarity
Biological Process (7 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in oogenesis
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Summaries
    Protein Function (UniProtKB)
    May have a regulatory function.
    (UniProt, P13468)
    Phenotypic Description (Red Book; Lindsley and Zimm 1992)
    fs(1)K10
    Homozygous females lay eggs with hyperplasia of the anterior chorionic appendages to form a collar around the micropile. Pole-cell-transplantation studies demonstrate chorionic phenotype to depend on germ-line genotype; pattern of overlying follicle cells apparently depends on cues from oocyte. In situ hybridization and antibody staining indicate that expression is confined to the primary oocyte and that the protein product is sequestered in the oocyte nucleus (Prost, Deryckere, Roos, Haenlin, Pantesco, and Mohier, 1988, Genes Dev. 2: 891-900). Eggs of mutant females seldom fertilized; those that are exhibit abnormalities of gastrulation with the anterior ends showing dorsal patterns of development both ventrally and literally. Some larvae produced with dorsal cuticular pattern covering entire circumference; last few segments have normal ventral hypoderm pattern. Ventralizing mutants grk and top both epistatic to the dorsalizing effects of fs(1)K10 (Schupbach, 1987, Cell 49: 699-707). Germ-line mosaics produced by mitotic exchange indicate fs(1)K10 activity required during oogenesis (Marsh, Wieschaus, and Gehring, 1976, Experientia 32: 803). Ovarian clones of homozygous cells useful in investigating kinetics of oogenesis (Wieschaus and Szabad, 1979, Dev. Biol. 68: 29-46).
    Gene Model and Products
    Number of Transcripts
    1
    Number of Unique Polypeptides
    1

    Please see the JBrowse view of Dmel\fs(1)K10 for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Comments on Gene Model

    Gene model reviewed during 5.51

    Low-frequency RNA-Seq exon junction(s) not annotated.

    Sequence Ontology: Class of Gene
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    Assoc. CDS (aa)
    FBtr0070396
    3052
    463
    Additional Transcript Data and Comments
    Reported size (kB)

    6, 4, 3.1 (northern blot)

    Comments
    External Data
    Crossreferences
    Rfam - A collection of RNA sequence families of structural RNAs including non-coding RNA genes as well as cis-regulatory elements
    Polypeptide Data
    Annotated Polypeptides
    Name
    FlyBase ID
    Predicted MW (kDa)
    Length (aa)
    Theoretical pI
    RefSeq ID
    GenBank
    FBpp0070380
    51.3
    463
    11.04
    Polypeptides with Identical Sequences

    There is only one protein coding transcript and one polypeptide associated with this gene

    Additional Polypeptide Data and Comments
    Reported size (kDa)

    463 (aa); 51.5 (kD predicted)

    Comments

    The predicted fs(1)K10 protein contains a putative helix-loop-helix DNA binding domain.

    External Data
    Crossreferences
    Linkouts
    Sequences Consistent with the Gene Model
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\fs(1)K10 using the Feature Mapper tool.

    External Data
    Crossreferences
    Linkouts
    Expression Data
    Expression Summary Ribbons
    Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
    For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
    Transcript Expression
    No Assay Recorded
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    in situ
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    northern blot
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    radioisotope in situ
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    Additional Descriptive Data

    The 3.1 kb transcript is detected at highest levels in ovaries and early embryos. It is detected at 20 to 50-fold lower levels throughout the rest of development.

    Marker for
     
    Subcellular Localization
    CV Term
    Polypeptide Expression
    immunolocalization
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    Additional Descriptive Data

    fs(1)K10 protein is first detected at low levels around the oocyte nucleus in stage S8-S9 egg chambers. The signal subsequently intensifies and becomes nuclear. After stage S12, the signal is no longer detected.

    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    located_in nucleus
    inferred from direct assay
    Expression Deduced from Reporters
    High-Throughput Expression Data
    Associated Tools

    GBrowse - Visual display of RNA-Seq signals

    View Dmel\fs(1)K10 in GBrowse 2
    RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    Images
    Alleles, Insertions, Transgenic Constructs, and Aberrations
    Classical and Insertion Alleles ( 20 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 62 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of fs(1)K10
    Transgenic constructs containing regulatory region of fs(1)K10
    Aberrations (Deficiencies and Duplications) ( 19 )
    Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Lethality
    Allele
    Sterility
    Allele
    Other Phenotypes
    Allele
    Phenotype manifest in
    Allele
    Orthologs
    Human Orthologs (via DIOPT v8.0)
    Homo sapiens (Human) (1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    1 of 15
    Yes
    No
    Model Organism Orthologs (via DIOPT v8.0)
    Mus musculus (laboratory mouse) (1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    1 of 15
    Yes
    No
    Rattus norvegicus (Norway rat) (0)
    No records found.
    Xenopus tropicalis (Western clawed frog) (0)
    No records found.
    Danio rerio (Zebrafish) (0)
    No records found.
    Caenorhabditis elegans (Nematode, roundworm) (2)
    1 of 15
    Yes
    Yes
    1 of 15
    Yes
    Yes
    Arabidopsis thaliana (thale-cress) (0)
    No records found.
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    No records found.
    Schizosaccharomyces pombe (Fission yeast) (0)
    No records found.
    Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190HNM )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila suzukii
    Spotted wing Drosophila
    Drosophila simulans
    Drosophila sechellia
    Drosophila erecta
    Drosophila yakuba
    Drosophila ananassae
    Drosophila pseudoobscura pseudoobscura
    Drosophila persimilis
    Drosophila willistoni
    Drosophila willistoni
    Drosophila virilis
    Drosophila mojavensis
    Drosophila grimshawi
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150JLO )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Musca domestica
    House fly
    Musca domestica
    House fly
    Glossina morsitans
    Tsetse fly
    Mayetiola destructor
    Hessian fly
    Anopheles darlingi
    American malaria mosquito
    Anopheles gambiae
    Malaria mosquito
    Culex quinquefasciatus
    Southern house mosquito
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0PFR )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Dendroctonus ponderosae
    Mountain pine beetle
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
    No non-Insect Arthropod orthologies identified
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G15PH )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Strongylocentrotus purpuratus
    Purple sea urchin
    Paralogs
    Paralogs (via DIOPT v8.0)
    Drosophila melanogaster (Fruit fly) (1)
    1 of 10
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Model Summary Ribbon
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Interactions Browser

      Please see the Physical Interaction reports below for full details
      RNA-protein
      Physical Interaction
      Assay
      References
      RNA-RNA
      Physical Interaction
      Assay
      References
      protein-protein
      Physical Interaction
      Assay
      References
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement

      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      External Data
      Linkouts
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      X
      Recombination map

      1-0.9

      Cytogenetic map
      Sequence location
      X:2,242,138..2,246,041 [+]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      2F1-2F2
      Limits computationally determined from genome sequence between P{EP}EP1460&P{EP}CG2924EP1596 and P{EP}EP1606
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      2F1-2F2
      (determined by in situ hybridisation)
      Determined by deficiency mapping (details unspecified).
      Experimentally Determined Recombination Data
      Location
      Left of (cM)
      Right of (cM)
      Notes

      Mapping based on 191 recombinants between y and w.

      Stocks and Reagents
      Stocks (7)
      Genomic Clones (15)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (60)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       

      polyclonal

      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for database merge of

      Source for merge of: fs(1)K10 CG3218

      Additional comments
      Other Comments

      The sdt non-coding regions contains a Transport/localisation sequence (TLS) - residues 52-95, required for normal localisation.

      In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.

      Ectopic expression of Cf2 counteracts the dorsalising effect of fs(1)K10 mutants.

      Transport and early localisation activities of fs(1)K10, bcd and osk mRNAs are remarkably similar to each other suggesting the mRNAs interact with a common set of microtubule based motor proteins and associated factors.

      Anterior localisation of RNA during oogenesis is very sensitive to microtubule inhibitors, taxol and other microtubule depolymerising agents. These results, together with colchicine treatment studies, demonstrate that microtubules are required for RNA transport to the oocyte.

      The cis regulatory regions of fs(1)K10 mRNA localisation are identified and characterised. A 44 nucleotide sequence, the transport/localisation sequence (TLS), is essential to fs(1)K10 function and is necessary and sufficient for mRNA transport into and anterior localisation within the oocyte.

      capu and spir are not required for the synthesis or nuclear localisation of fs(1)K10 protein and they mediate dorsoventral patterning independently of fs(1)K10 mRNA localisation.

      grk behaves as a dominant partial suppressor of fs(1)K10.

      Displays epistatic interactions with sqd alleles.

      ve expression pattern in embryogenesis is altered in fs(1)K10 mutants.

      The mechanism of fs(1)K10 RNA accumulation in the oocyte has been studied.

      fs(1)K10 expression patterns were investigated in egl embryos to determine the relationship between fs(1)K10 and egl.

      A female germ line mosaic assay using a fs(1)K10 mutation as a marker indicates that sevin (a carbamate insecticide) is mutagenic/recombinogenic in D.melanogaster.

      Nuclear localisation of fs(1)K10 protein and the putative DNA-binding motif within the protein suggest fs(1)K10 might possess a regulatory function.

      P-element transformation experiments determine the functional fs(1)K10 gene resides between position -1.2 and +3.4.

      Mutations at fs(1)K10 causes dorsalised embryonic phenotypes and egg chambers.

      fs(1)K10 has been cloned by the use of microdissection, chromosome walking and cosmid libraries constructed in a P element vector.

      Homozygous females lay eggs with hyperplasia of the anterior chorionic appendages to form a collar around the micropile. Pole-cell-transplantation studies demonstrate chorionic phenotype to depend on germ-line genotype; pattern of overlying follicle cells apparently depends on cues from oocyte. In situ hybridization and antibody staining indicate that expression is confined to the primary oocyte and that the protein product is sequestered in the oocyte nucleus (Prost, Deryckere, Roos, Haenlin, Pantesco and Mohier, 1988). Eggs of mutant females seldom fertilized; those that are exhibit abnormalities of gastrulation with the anterior ends showing dorsal patterns of development both ventrally and literally. Some larvae produced with dorsal cuticular pattern covering entire circumference; last few segments have normal ventral hypoderm pattern. Ventralizing mutants grk and top both epistatic to the dorsalizing effects of fs(1)K10 (Schupbach, 1987). Germ-line mosaics produced by mitotic exchange indicate fs(1)K10 activity required during oogenesis (Marsh et al., 1976). Ovarian clones of homozygous cells useful in investigating kinetics of oogenesis (Wieschaus and Szabad, 1979).

      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 38 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      Other crossreferences
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      Rfam - A collection of RNA sequence families of structural RNAs including non-coding RNA genes as well as cis-regulatory elements
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DPiM - Drosophila Protein interaction map
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyMine - An integrated database for Drosophila genomics
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Synonyms and Secondary IDs (10)
      Reported As
      Symbol Synonym
      Name Synonyms
      female sterile (1) K10
      Secondary FlyBase IDs
      • FBgn0029607
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (223)