K10, fs(1)M9, EG:30B8.5
Please see the JBrowse view of Dmel\fs(1)K10 for information on other features
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Gene model reviewed during 5.51
Low-frequency RNA-Seq exon junction(s) not annotated.
6, 4, 3.1 (northern blot)
There is only one protein coding transcript and one polypeptide associated with this gene
463 (aa); 51.5 (kD predicted)
The predicted fs(1)K10 protein contains a putative helix-loop-helix DNA binding domain.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\fs(1)K10 using the Feature Mapper tool.
Comment: 0-12 hr AEL
The 3.1 kb transcript is detected at highest levels in ovaries and early embryos. It is detected at 20 to 50-fold lower levels throughout the rest of development.
fs(1)K10 protein is first detected at low levels around the oocyte nucleus in stage S8-S9 egg chambers. The signal subsequently intensifies and becomes nuclear. After stage S12, the signal is no longer detected.
GBrowse - Visual display of RNA-Seq signals
View Dmel\fs(1)K10 in GBrowse 21-0.9
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: fs(1)K10 CG3218
The sdt non-coding regions contains a Transport/localisation sequence (TLS) - residues 52-95, required for normal localisation.
In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
Anterior localisation of RNA during oogenesis is very sensitive to microtubule inhibitors, taxol and other microtubule depolymerising agents. These results, together with colchicine treatment studies, demonstrate that microtubules are required for RNA transport to the oocyte.
Displays epistatic interactions with sqd alleles.
ve expression pattern in embryogenesis is altered in fs(1)K10 mutants.
The mechanism of fs(1)K10 RNA accumulation in the oocyte has been studied.
fs(1)K10 expression patterns were investigated in egl embryos to determine the relationship between fs(1)K10 and egl.
A female germ line mosaic assay using a fs(1)K10 mutation as a marker indicates that sevin (a carbamate insecticide) is mutagenic/recombinogenic in D.melanogaster.
Mutations at fs(1)K10 causes dorsalised embryonic phenotypes and egg chambers.
fs(1)K10 has been cloned by the use of microdissection, chromosome walking and cosmid libraries constructed in a P element vector.
Homozygous females lay eggs with hyperplasia of the anterior chorionic appendages to form a collar around the micropile. Pole-cell-transplantation studies demonstrate chorionic phenotype to depend on germ-line genotype; pattern of overlying follicle cells apparently depends on cues from oocyte. In situ hybridization and antibody staining indicate that expression is confined to the primary oocyte and that the protein product is sequestered in the oocyte nucleus (Prost, Deryckere, Roos, Haenlin, Pantesco and Mohier, 1988). Eggs of mutant females seldom fertilized; those that are exhibit abnormalities of gastrulation with the anterior ends showing dorsal patterns of development both ventrally and literally. Some larvae produced with dorsal cuticular pattern covering entire circumference; last few segments have normal ventral hypoderm pattern. Ventralizing mutants grk and top both epistatic to the dorsalizing effects of fs(1)K10 (Schupbach, 1987). Germ-line mosaics produced by mitotic exchange indicate fs(1)K10 activity required during oogenesis (Marsh et al., 1976). Ovarian clones of homozygous cells useful in investigating kinetics of oogenesis (Wieschaus and Szabad, 1979).
