K10, EG:30B8.5 , fs(1)M9
Gene model reviewed during 5.51
Low-frequency RNA-Seq exon junction(s) not annotated.
6, 4, 3.1 (northern blot)
There is only one protein coding transcript and one polypeptide associated with this gene
463 (aa); 51.5 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\fs(1)K10 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\fs(1)K10 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: fs(1)K10 CG3218
The sdt non-coding regions contains a Transport/localisation sequence (TLS) - residues 52-95, required for normal localisation.
In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
Transport and early localisation activities of fs(1)K10, bcd and osk mRNAs are remarkably similar to each other suggesting the mRNAs interact with a common set of microtubule based motor proteins and associated factors.
Anterior localisation of RNA during oogenesis is very sensitive to microtubule inhibitors, taxol and other microtubule depolymerising agents. These results, together with colchicine treatment studies, demonstrate that microtubules are required for RNA transport to the oocyte.
The cis regulatory regions of fs(1)K10 mRNA localisation are identified and characterised. A 44 nucleotide sequence, the transport/localisation sequence (TLS), is essential to fs(1)K10 function and is necessary and sufficient for mRNA transport into and anterior localisation within the oocyte.
ve expression pattern in embryogenesis is altered in fs(1)K10 mutants.
fs(1)K10 expression patterns were investigated in egl embryos to determine the relationship between fs(1)K10 and egl.
Mutations at fs(1)K10 causes dorsalised embryonic phenotypes and egg chambers.
fs(1)K10 has been cloned by the use of microdissection, chromosome walking and cosmid libraries constructed in a P element vector.
Homozygous females lay eggs with hyperplasia of the anterior chorionic appendages to form a collar around the micropile. Pole-cell-transplantation studies demonstrate chorionic phenotype to depend on germ-line genotype; pattern of overlying follicle cells apparently depends on cues from oocyte. In situ hybridization and antibody staining indicate that expression is confined to the primary oocyte and that the protein product is sequestered in the oocyte nucleus (Prost, Deryckere, Roos, Haenlin, Pantesco and Mohier, 1988). Eggs of mutant females seldom fertilized; those that are exhibit abnormalities of gastrulation with the anterior ends showing dorsal patterns of development both ventrally and literally. Some larvae produced with dorsal cuticular pattern covering entire circumference; last few segments have normal ventral hypoderm pattern. Ventralizing mutants grk and top both epistatic to the dorsalizing effects of fs(1)K10 (Schupbach, 1987). Germ-line mosaics produced by mitotic exchange indicate fs(1)K10 activity required during oogenesis (Marsh et al., 1976). Ovarian clones of homozygous cells useful in investigating kinetics of oogenesis (Wieschaus and Szabad, 1979).