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General Information
Symbol
Dmel\Gapdh1
Species
D. melanogaster
Name
Glyceraldehyde 3 phosphate dehydrogenase 1
Annotation Symbol
CG12055
Feature Type
FlyBase ID
FBgn0001091
Gene Model Status
Stock Availability
Enzyme Name (EC)
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (1.2.1.12)
Gene Snapshot
Glyceraldehyde 3 phosphate dehydrogenase 1 (Gapdh1) encodes a glyceraldehyde-3-phosphate dehydrogenase involved in glycolysis, myoblast fusion and the development of somatic muscle. [Date last reviewed: 2019-09-19]
Also Known As
GAPDH, glyceraldehyde-3-phosphate dehydrogenase, Gapdh-1, GADPH
Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:7,791,901..7,793,380 [-]
Recombination map
2-57
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. (P07486)
Catalytic Activity (EC)
Experimental Evidence
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH (1.2.1.12)
Predictions / Assertions
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH (1.2.1.12)
Summaries
Gene Group (FlyBase)
ALDEHYDE OR OXO OXIDOREDUCTASES, NAD OR NADP AS ACCEPTOR -
Aldehyde or oxo oxidoreductases with NAD or NADP as acceptor, include dehydrogenases that oxidize an aldehyde or ketone (oxo) group with the reduction of NAD or NADP.
PEPTIDYL-CYSTEINE S-NITROSYLASES -
Peptidyl-cysteine S-nitrosylases are transferases that are involved in the transfer of nitric oxide group to a sulfur atom within a cysteine residue of a protein.
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Gapdh1: Glyceraldehyde dehydrogenase 1
One of two genes (Gapdh1 and Gapdh2) that code for the enzyme glyceraldehyde-3-phosphate dehydrogenase [G3PD (EC.1.2.12)]. These genes are expressed in almost all cells, especially those that undergo a high rate of glycolysis.
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\Gapdh1 or the JBrowse view of Dmel\Gapdh1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.49
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0088903
1480
332
FBtr0100479
1330
332
Additional Transcript Data and Comments
Reported size (kB)
1.6 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0087977
35.4
332
8.42
FBpp0099914
35.4
332
8.42
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

332 aa isoforms: Gapdh1-PA, Gapdh1-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)
Homotetramer.
(UniProt, P07486)
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Gapdh1 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Gene Ontology (10 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000089473
(assigned by GO_Central )
inferred from electronic annotation with InterPro:IPR006424
(assigned by InterPro )
inferred from electronic annotation with InterPro:IPR006424
(assigned by InterPro )
inferred from sequence or structural similarity with UniProtKB:P04797
Biological Process (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from expression pattern
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR006424
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN000089473
(assigned by GO_Central )
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000089473
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism | ubiquitous

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Glycolytic enzymes, including Gpdh protein are localized to M bands and Z discs, as visualized in the isolated myofibrils of flight muscles, where immunoreactivity appears as alternating bright and less-intense bands.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Gapdh1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 3 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Gapdh1
Transgenic constructs containing regulatory region of Gapdh1
Deletions and Duplications ( 5 )
Phenotypes
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
 
7 of 15
No
Yes
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
8 of 15
Yes
Yes
8 of 15
Yes
Yes
7 of 15
No
Yes
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (7)
11 of 13
Yes
Yes
7 of 13
No
Yes
6 of 13
No
Yes
6 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (1)
7 of 12
Yes
Yes
Danio rerio (Zebrafish) (3)
13 of 15
Yes
Yes
5 of 15
No
Yes
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (4)
13 of 15
Yes
Yes
12 of 15
No
Yes
12 of 15
No
No
12 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (7)
8 of 9
Yes
Yes
8 of 9
Yes
Yes
7 of 9
No
Yes
6 of 9
No
Yes
1 of 9
No
Yes
1 of 9
No
Yes
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (3)
14 of 15
Yes
Yes
14 of 15
Yes
No
12 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (2)
12 of 12
Yes
Yes
12 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190BN4 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila simulans
Drosophila sechellia
Drosophila sechellia
Drosophila sechellia
Drosophila erecta
Drosophila erecta
Drosophila yakuba
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150831 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W08M6 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X08HR )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0B1Y )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (2)
9 of 10
6 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Homotetramer.
    (UniProt, P07486 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-57
    Cytogenetic map
    Sequence location
    2R:7,791,901..7,793,380 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    43E17-43E17
    Limits computationally determined from genome sequence between P{EP}CanB2EP774 and P{lacW}lin19k01207&P{lacW}rnh1k07624
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    43E-43F
    (determined by in situ hybridisation)
    43E-43E
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (7)
    Genomic Clones (15)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (308)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for database merge of
      Additional comments
      The Gapdh1 gene may have been derived from the Gapdh2 gene by retroposition.
      Other Comments
      Identified with: GH12586 <up>FlyBase curator comment: EST subsequently found to be chimeric</up>.
      At least a substantial fraction of three glycolytic enzymes of flight muscle cells are found colocalised at the Z-discs and M-lines, glycolytic enzyme colocalisation is interdependent. Localisation of Gapdh1, Gapdh2 and Ald at the Z-discs and M-lines requires the presence of at least Gpdh at the Z-discs and M-lines.
      EST GH12586 is chimeric; the 5' portion (AI134984) corresponds to part of CG4461, the 3' portion (BG632702) corresponds to part of Gapdh1.
      The synergistic effects of the Adh locus, or a tightly linked locus, on the quantities of the Mdh1, Gpdh, Pgm, Idh, Men and Gapdh1 gene products in D.melanogaster derived from an African population has been determined.
      The melanogaster and takahashi subgroups of the melanogaster group have small and large subunits of Gapdh, while another 28 species (in 15 subgroups) studied were found to have only either the large or the small subunit of Gapdh.
      Gapdh1 and Gapdh2 RNA expression has been studied.
      One of two genes (Gapdh1 and Gapdh2) that code for the enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH). These genes are expressed in almost all cells, especially those that undergo a high rate of glycolysis.
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 54 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      KEGG Genes - Molecular building blocks of life in the genomic space.
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      modMine - A data warehouse for the modENCODE project
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DPiM - Drosophila Protein interaction map
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyMine - An integrated database for Drosophila genomics
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Synonyms and Secondary IDs (26)
      Reported As
      Symbol Synonym
      BEST:GH12586
      Gapdh43E
      Name Synonyms
      G3-P dehydrogenase
      Glyceraldehyde 3 phosphate dehydrogenase
      Glyceraldehyde 3 phosphate dehydrogenase 1
      Glyceraldehyde-3-phosphate dehydrogenase
      Glyceraldehyde-3-phosphate dehydrogenase1
      glyceraldehyde 3 phosphate dehydrogenase1
      Secondary FlyBase IDs
      • FBgn0043822
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (134)