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Gene Dmel\Gapdh2

General Information
Symbol	Dmel\Gapdh2	Species	D. melanogaster
Name	Glyceraldehyde 3 phosphate dehydrogenase 2	Annotation symbol	CG8893
Feature type	protein_coding_gene	FlyBase ID	FBgn0001092
Gene Model Status	Current	Stock availability	7 publicly available
Also Known As	GAPDH, Gapdh-2
Genomic Location
Chromosome (arm)	X	Recombination map	1-
Cytogenetic map	13F17-13F17	Sequence location	X:15,762,178..15,763,694 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Glyceraldehyde 3 phosphate dehydrogenase 2 is referred to in FlyBase by the symbol Dmel\Gapdh2 (CG8893, FBgn0001092). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity. There is experimental evidence that it is involved in the biological process: glycolysis. 9 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Glyceraldehyde 3-phosphate dehydrogenase, NAD(P) binding domain; Glyceraldehyde 3-phosphate dehydrogenase, active site; Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain; Glyceraldehyde-3-phosphate dehydrogenase, type I; Glyceraldehyde/Erythrose phosphate dehydrogenase family; NAD(P)-binding domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of moderate expression. Peak expression observed at stages throughout the larval period. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Gene sequence location is X:15762178..15763694.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Gapdh2: Glyceraldehyde dehydrogenase 2 Another gene that codes for the enzyme glyceraldehyde-3-phosphate dehydrogenase (see Gapdh1). The level of expression of Gapdh2 has been found to be regulated developmentally (Sun et al., 1988).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 13F17-13F17   Limits computationally determined from genome sequence between P{EP}Gβ13FEP1071 and P{EP}EP1458&P{EP}EP1522
Experimentally Determined Cytological Location
Cytogenetic map Notes References 13F-13F   (determined by in situ hybridisation)   (Tso et al., 1985)
Experimentally Determined Recombination Data
Location	1- (Choudhary et al., 1992)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Gapdh2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50 Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.45
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Gapdh2-RA FBtr0074112  NM_080714   1443   332 Gapdh2-RC FBtr0332618  NM_001272655   1377   332
Additional Transcript Data & Comments
Reported size (kB)	1.6 (northern blot) (Tso et al., 1985)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Gapdh2-PA   FBpp0073922   35.4   332   8.72     NP_542445   AAF48531 Gapdh2-PC   FBpp0304864   35.4   332   8.72     NP_001259584   AGB95426
Additional Polypeptide Data & Comments
Reported size (kDa)	332 (aa) (Tso et al., 1985)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Glyceraldehyde-3-phosphate dehydrogenase, type I (IPR006424) Glyceraldehyde 3-phosphate dehydrogenase, NAD(P) binding domain (IPR020828) Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain (IPR020829) Glyceraldehyde 3-phosphate dehydrogenase, active site (IPR020830) Glyceraldehyde/Erythrose phosphate dehydrogenase family (IPR020831) NAD(P)-binding domain (IPR016040)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA246353   AA567429   AA567821   AA696494   AA697448   AA697830   AA698441   AA698635   AA698636   AA698888   AA735624   AA817090   AA941166   AE014298 AAF48531   AC092401   AE014298 AGB95426   AI064388   AI064482   AI064625   AI107120   AI107339   AI107363   AI113922   AI114307   AI135129   AI135254   AI135565   AI135699   AI135783   AI135844   AI256941   AI258249   AI259573   AI260557   AI260691   AI261026   AI292619   AI292630   AI294349   AI386646   AI387385   AI387714   AI387950   AI388071   AI388347   AI389028   AI389255   AI389690   AI390053   AI403191   AI404134   AI404238   AI514003   AI514739   AI516141   AI517745   AI518748   AI531762   AI531893   AI534986   AI543999   AI945061   AK284686   AY089643   AY094940 AAM11293   AY231704   AY441207   BF504437   BG635829   BG640301   BG640376   BI164868   BI166314   BI167488   BI216649   BI230953   BI232089   BI232874   BI234054   BI234589   BI235851   BI239065   BI242570   BI352139   BI353313   BI356035   BI369980   BI372409   BI485202   BI563722   BI563926   BI563986   BI564364   BI564563   BI564814   BI565277   BI565690   BI567123   BI567331   BI567389   BI567409   BI567752   BI567895   BI567902   BI568183   BI568251   BI568322   BI568734   BI569698   BI569759   BI569918   BI570007   BI570164   BI570545   BI570605   BI570614   BI570624   BI570792   BI570861   BI571395   BI571746   BI572513   BI572536   BI572682   BI574742   BI574885   BI575161   BI575941   BI576889   BI579196   BI581536   BI581859   BI581895   BI581936   BI582109   BI582439   BI583112   BI583670   BI583745   BI584075   BI585541   BI585678   BI586719   BI586817   BI586986   BI587369   BI587746   BI588660   BI588737   BI588902   BI589416   BI589574   BI590381   BI590717   BI591558   BI592065   BI592153   BI592194   BI592333   BI592363   BI593393   BI605163   BI605291   BI605762   BI606108   BI606264   BI606592   BI607324   BI607335   BI607998   BI608478   BI608501   BI608537   BI609312   BI609330   BI609530   BI609757   BI610077   BI610136   BI610594   BI610762   BI610770   BI610885   BI611909   BI612526   BI613293   BI613622   BI614195   BI614201   BI614531   BI614571   BI614711   BI614995   BI616132   BI616244   BI616431   BI617527   BI618226   BI618355   BI618741   BI618795   BI618875   BI619151   BI619186   BI619199   BI619211   BI619439   BI619624   BI619898   BI620018   BI620701   BI620977   BI621010   BI621640   BI621933   BI622026   BI622166   BI622330   BI622369   BI622508   BI622568   BI623576   BI623912   BI624927   BI625336   BI625371   BI625484   BI625731   BI626482   BI626499   BI626504   BI626634   BI626861   BI626969   BI627053   BI627134   BI627208   BI627299   BI627489   BI627895   BI628149   BI628268   BI628444   BI628587   BI629276   BI630822   BI631176   BI631712   BI634098   BI634173   BI634179   BI634457   BI634524   BI634782   BI636868   BP542698   BP548633   BP549350   BP549351   BP550050   BP550051   BP551357   BP551358   BP553004   BQ103499   BT004485   BX008333   BX012207   BX014553   BX015882   BX015886   BX022772   BX031723   BX032235   BX032633   BX033087   BX033362   BX034622   BX057023   BX057309   BX066162   BX068467   CA803176   CA803253   CA803435   CA806692   CK659320   CK659957   CK662321   CO152748   CO180375   CO181836   CO182117   CO192692   CO264529   CO267119   CO270028   CO274240   CO300572   CO321781   CO322922   EC059578   EC060418   EC060931   EC074587   EC082672   EC197992   EC223294   EC225840   EC246073   EC246965   EC262618   EL881867   EL881868   EL881983   EL881984   EL882096   EL882218   EL882219   EL883899   ES691512   ES691945   ES691984   ES694175   ES694723   ES694740   ES694765   ES696502   ES696504   EV577440   EV579021   EV579291   EV579691   EV580676   EV582305   EV583203   EV583833   EV584222   EV585023   EV585343   EV587017   EV587071   EV588124   EV590624   EV596565   EV600668   GH747714   GH749159   GH750438   GH750625   GH750682   GH750932   GH753029   GH753624   GH755204   GH756017   GH757135   GH757778   GH758365   GH758656   GH761143   GH770250   GH770336   GH772196   GH772660   GH773303   GH777132   GH777575   GH778132   GH778601   GH779092   GH780050   GH780634   GH781556   GH782158   GH786888   GH789435   GH792543   GH793263   GH794010   GH795778   GH795795   GH796129   GH796527   GH796985   GH797291   GH799288   GH799442   GH799781   GH800413   GH800447   GH803913   GH806640   GH806790   GH809409   GH810458   GH813748   GH817787   GH818238   GH819865   GH821523   GH824013   GH824501   GH825072   GH827928   GH828664   GH831618   GH831730   GH833667   GH834467   GH835126   GH836384   GH837466   GH838100   GH838956   GH840245   GH840824   GH845170   GH845733   GH846252   GH849060   GH849475   GH851015   GH852802   GH854103   GH854578   GH855435   GH856031   GH856567   GH858870   GH858996   GH859972   GH860357   GH860667   GH862618   GH862898   GH863367   GH864821   GH865650   GH866491   GH867055   GH867148   GH867795   GH868032   GH868041   GH869166   GH871026   GH872040   GH875766   GH875939   GH876535   GH877579   GH878813   GH879578   GH882162   GH885520   GH886496   GH886870   GH887514   GH888066   GH888975   GH892834   GH893161   GH895976   GH897687   GH897784   GH899364   GH899796   GH900172   GH900432   GH900593   GH901295   GH901310   GH907492   GH908762   GH909204   GH909857   GH910793   GH911134   GH912310   GH912919   GH912982   GH914633   GH915377   GH917898   GH918207   GH919772   GH920376   GH923637   GH923885   GH925920   GH926324   GH926496   GH928023   GH930530   GH931660   GH931672   GH932305   GH932779   GH933677   GH935741   GH936052   GH936260   GH938351   GH938762   GH942306   M11255 AAA28561
UniProtKB/Swiss-Prot	P07487
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences	EPD - Eukarytoic Promoter Database, an annotated collection of POL II promoters • 23032
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference adult stage     (Tso et al., 1985)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Dorus et al., 2006) day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
lipid particle inferred from direct assay (Beller et al., 2006, Beller et al., 2006) microtubule associated complex inferred from direct assay (Hughes et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    277 central nervous system, larvae L3    224 central nervous system, pupae P8    185 head, virgin 1-day female    797 head, virgin 4-day female    445 head, virgin 20-day female    419 head, mated 1-day female    533 head, mated 4-day female    312 head, mated 20-day female    306 head, mated 1-day male    741 head, mated 4-day male    437 head, mated 20-day male    596 salivary gland, larvae L3 wandering    46 salivary gland, white prepupae    92 digestive system, larvae L3 wandering    133 digestive system, 1-day adult    399 digestive system, 4-day adult    284 digestive system, 20-day adult    338 fat body, larvae L3 wandering    235 fat body, white prepupae    289 fat body, pupae P8    136 carcass, larvae L3 wandering    418 carcass, 1-day adult    920 carcass, 4-day adult    463 carcass, 20-day adult    349 ovary, virgin 4-day female    1027 ovary, mated 4-day female    1014 testis, mated 4-day male    92 accessory gland, mated 4-day male    127 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (277) central nervous system, larvae L3  (224) central nervous system, pupae P8  (185) head, virgin 1-day female  (797) head, virgin 4-day female  (445) head, virgin 20-day female  (419) head, mated 1-day female  (533) head, mated 4-day female  (312) head, mated 20-day female  (306) head, mated 1-day male  (741) head, mated 4-day male  (437) head, mated 20-day male  (596) salivary gland, larvae L3 wandering  (46) salivary gland, white prepupae  (92) digestive system, larvae L3 wandering  (133) digestive system, 1-day adult  (399) digestive system, 4-day adult  (284) digestive system, 20-day adult  (338) fat body, larvae L3 wandering  (235) fat body, white prepupae  (289) fat body, pupae P8  (136) carcass, larvae L3 wandering  (418) carcass, 1-day adult  (920) carcass, 4-day adult  (463) carcass, 20-day adult  (349) ovary, virgin 4-day female  (1027) ovary, mated 4-day female  (1014) testis, mated 4-day male  (92) accessory gland, mated 4-day male  (127) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (277) central nervous system, larvae L3  (224) central nervous system, pupae P8  (185) head, virgin 1-day female  (797) head, virgin 4-day female  (445) head, virgin 20-day female  (419) head, mated 1-day female  (533) head, mated 4-day female  (312) head, mated 20-day female  (306) head, mated 1-day male  (741) head, mated 4-day male  (437) head, mated 20-day male  (596) salivary gland, larvae L3 wandering    46 salivary gland, white prepupae    92 digestive system, larvae L3 wandering  (133) digestive system, 1-day adult  (399) digestive system, 4-day adult  (284) digestive system, 20-day adult  (338) fat body, larvae L3 wandering  (235) fat body, white prepupae  (289) fat body, pupae P8  (136) carcass, larvae L3 wandering  (418) carcass, 1-day adult  (920) carcass, 4-day adult  (463) carcass, 20-day adult  (349) ovary, virgin 4-day female  (1027) ovary, mated 4-day female  (1014) testis, mated 4-day male    92 accessory gland, mated 4-day male  (127) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    277 central nervous system, larvae L3    224 central nervous system, pupae P8    185 head, virgin 1-day female    797 head, virgin 4-day female    445 head, virgin 20-day female    419 head, mated 1-day female    533 head, mated 4-day female    312 head, mated 20-day female    306 head, mated 1-day male    741 head, mated 4-day male    437 head, mated 20-day male    596 salivary gland, larvae L3 wandering    46 salivary gland, white prepupae    92 digestive system, larvae L3 wandering    133 digestive system, 1-day adult    399 digestive system, 4-day adult    284 digestive system, 20-day adult    338 fat body, larvae L3 wandering    235 fat body, white prepupae    289 fat body, pupae P8    136 carcass, larvae L3 wandering    418 carcass, 1-day adult    920 carcass, 4-day adult    463 carcass, 20-day adult    349 ovary, virgin 4-day female    1027 ovary, mated 4-day female    1014 testis, mated 4-day male    92 accessory gland, mated 4-day male    127 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    277 central nervous system, larvae L3    224 central nervous system, pupae P8    185 head, virgin 1-day female    797 head, virgin 4-day female    445 head, virgin 20-day female    419 head, mated 1-day female    533 head, mated 4-day female    312 head, mated 20-day female    306 head, mated 1-day male    741 head, mated 4-day male    437 head, mated 20-day male    596 salivary gland, larvae L3 wandering    46 salivary gland, white prepupae    92 digestive system, larvae L3 wandering    133 digestive system, 1-day adult    399 digestive system, 4-day adult    284 digestive system, 20-day adult    338 fat body, larvae L3 wandering    235 fat body, white prepupae    289 fat body, pupae P8    136 carcass, larvae L3 wandering    418 carcass, 1-day adult    920 carcass, 4-day adult    463 carcass, 20-day adult    349 ovary, virgin 4-day female    1027 ovary, mated 4-day female    1014 testis, mated 4-day male    92 accessory gland, mated 4-day male    127 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (277) central nervous system, larvae L3  (224) central nervous system, pupae P8  (185) head, virgin 1-day female  (797) head, virgin 4-day female  (445) head, virgin 20-day female  (419) head, mated 1-day female  (533) head, mated 4-day female  (312) head, mated 20-day female  (306) head, mated 1-day male  (741) head, mated 4-day male  (437) head, mated 20-day male  (596) salivary gland, larvae L3 wandering  (46) salivary gland, white prepupae  (92) digestive system, larvae L3 wandering  (133) digestive system, 1-day adult  (399) digestive system, 4-day adult  (284) digestive system, 20-day adult  (338) fat body, larvae L3 wandering  (235) fat body, white prepupae  (289) fat body, pupae P8  (136) carcass, larvae L3 wandering  (418) carcass, 1-day adult  (920) carcass, 4-day adult  (463) carcass, 20-day adult  (349) ovary, virgin 4-day female  (1027) ovary, mated 4-day female  (1014) testis, mated 4-day male  (92) accessory gland, mated 4-day male  (127) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (277) central nervous system, larvae L3  (224) central nervous system, pupae P8  (185) head, virgin 1-day female  (797) head, virgin 4-day female  (445) head, virgin 20-day female  (419) head, mated 1-day female  (533) head, mated 4-day female  (312) head, mated 20-day female  (306) head, mated 1-day male  (741) head, mated 4-day male  (437) head, mated 20-day male  (596) salivary gland, larvae L3 wandering    46 salivary gland, white prepupae    92 digestive system, larvae L3 wandering  133 digestive system, 1-day adult  (399) digestive system, 4-day adult  (284) digestive system, 20-day adult  (338) fat body, larvae L3 wandering  (235) fat body, white prepupae  (289) fat body, pupae P8  136 carcass, larvae L3 wandering  (418) carcass, 1-day adult  (920) carcass, 4-day adult  (463) carcass, 20-day adult  (349) ovary, virgin 4-day female  (1027) ovary, mated 4-day female  (1014) testis, mated 4-day male    92 accessory gland, mated 4-day male  127 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    277 central nervous system, larvae L3    224 central nervous system, pupae P8    185 head, virgin 1-day female    797 head, virgin 4-day female    445 head, virgin 20-day female    419 head, mated 1-day female    533 head, mated 4-day female    312 head, mated 20-day female    306 head, mated 1-day male    741 head, mated 4-day male    437 head, mated 20-day male    596 salivary gland, larvae L3 wandering    46 salivary gland, white prepupae    92 digestive system, larvae L3 wandering    133 digestive system, 1-day adult    399 digestive system, 4-day adult    284 digestive system, 20-day adult    338 fat body, larvae L3 wandering    235 fat body, white prepupae    289 fat body, pupae P8    136 carcass, larvae L3 wandering    418 carcass, 1-day adult    920 carcass, 4-day adult    463 carcass, 20-day adult    349 ovary, virgin 4-day female    1027 ovary, mated 4-day female    1014 testis, mated 4-day male    92 accessory gland, mated 4-day male    127 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of moderate expression. Peak expression observed at stages throughout the larval period. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    986 embryo 02-04hr    30 embryo 04-06hr    14 embryo 06-08hr    9 embryo 08-10hr    13 embryo 10-12hr    33 embryo 12-14hr    72 embryo 14-16hr    374 embryo 16-18hr    515 embryo 18-20hr    627 embryo 20-22hr    822 embryo 22-24hr    805 larva L1    1674 larva L2    1537 larva L3 12hr old    1521 larva L3 puffstage 1-2    1356 larva L3 puffstage 3-6    762 larva L3 puffstage 7-9    343 white prepupae new    355 white prepupae 12hr    208 white prepupae 24hr    158 pupae 2d postWPP    309 pupae 3d postWPP    664 pupae 4d postWPP    628 adult male 01day    786 adult male 05day    543 adult male 30day    383 adult female 01day    1119 adult female 05day    918 adult female 30day    869 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (986) embryo 02-04hr  (30) embryo 04-06hr    14 embryo 06-08hr    9 embryo 08-10hr    13 embryo 10-12hr  (33) embryo 12-14hr  (72) embryo 14-16hr  (374) embryo 16-18hr  (515) embryo 18-20hr  (627) embryo 20-22hr  (822) embryo 22-24hr  (805) larva L1  (1674) larva L2  (1537) larva L3 12hr old  (1521) larva L3 puffstage 1-2  (1356) larva L3 puffstage 3-6  (762) larva L3 puffstage 7-9  (343) white prepupae new  (355) white prepupae 12hr  (208) white prepupae 24hr  (158) pupae 2d postWPP  (309) pupae 3d postWPP  (664) pupae 4d postWPP  (628) adult male 01day  (786) adult male 05day  (543) adult male 30day  (383) adult female 01day  (1119) adult female 05day  (918) adult female 30day  (869) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (986) embryo 02-04hr    30 embryo 04-06hr    14 embryo 06-08hr    9 embryo 08-10hr    13 embryo 10-12hr    33 embryo 12-14hr    72 embryo 14-16hr  (374) embryo 16-18hr  (515) embryo 18-20hr  (627) embryo 20-22hr  (822) embryo 22-24hr  (805) larva L1  (1674) larva L2  (1537) larva L3 12hr old  (1521) larva L3 puffstage 1-2  (1356) larva L3 puffstage 3-6  (762) larva L3 puffstage 7-9  (343) white prepupae new  (355) white prepupae 12hr  (208) white prepupae 24hr  (158) pupae 2d postWPP  (309) pupae 3d postWPP  (664) pupae 4d postWPP  (628) adult male 01day  (786) adult male 05day  (543) adult male 30day  (383) adult female 01day  (1119) adult female 05day  (918) adult female 30day  (869) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    986 embryo 02-04hr    30 embryo 04-06hr    14 embryo 06-08hr    9 embryo 08-10hr    13 embryo 10-12hr    33 embryo 12-14hr    72 embryo 14-16hr    374 embryo 16-18hr    515 embryo 18-20hr    627 embryo 20-22hr    822 embryo 22-24hr    805 larva L1    1674 larva L2    1537 larva L3 12hr old    1521 larva L3 puffstage 1-2    1356 larva L3 puffstage 3-6    762 larva L3 puffstage 7-9    343 white prepupae new    355 white prepupae 12hr    208 white prepupae 24hr    158 pupae 2d postWPP    309 pupae 3d postWPP    664 pupae 4d postWPP    628 adult male 01day    786 adult male 05day    543 adult male 30day    383 adult female 01day    1119 adult female 05day    918 adult female 30day    869 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    986 embryo 02-04hr    30 embryo 04-06hr    14 embryo 06-08hr    9 embryo 08-10hr    13 embryo 10-12hr    33 embryo 12-14hr    72 embryo 14-16hr    374 embryo 16-18hr    515 embryo 18-20hr    627 embryo 20-22hr    822 embryo 22-24hr    805 larva L1    1674 larva L2    1537 larva L3 12hr old    1521 larva L3 puffstage 1-2    1356 larva L3 puffstage 3-6    762 larva L3 puffstage 7-9    343 white prepupae new    355 white prepupae 12hr    208 white prepupae 24hr    158 pupae 2d postWPP    309 pupae 3d postWPP    664 pupae 4d postWPP    628 adult male 01day    786 adult male 05day    543 adult male 30day    383 adult female 01day    1119 adult female 05day    918 adult female 30day    869 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (986) embryo 02-04hr  30 embryo 04-06hr    14 embryo 06-08hr    9 embryo 08-10hr    13 embryo 10-12hr  (33) embryo 12-14hr  (72) embryo 14-16hr  (374) embryo 16-18hr  (515) embryo 18-20hr  (627) embryo 20-22hr  (822) embryo 22-24hr  (805) larva L1  (1674) larva L2  (1537) larva L3 12hr old  (1521) larva L3 puffstage 1-2  (1356) larva L3 puffstage 3-6  (762) larva L3 puffstage 7-9  (343) white prepupae new  (355) white prepupae 12hr  (208) white prepupae 24hr  (158) pupae 2d postWPP  (309) pupae 3d postWPP  (664) pupae 4d postWPP  (628) adult male 01day  (786) adult male 05day  (543) adult male 30day  (383) adult female 01day  (1119) adult female 05day  (918) adult female 30day  (869) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (986) embryo 02-04hr    30 embryo 04-06hr    14 embryo 06-08hr    9 embryo 08-10hr    13 embryo 10-12hr    33 embryo 12-14hr    72 embryo 14-16hr  (374) embryo 16-18hr  (515) embryo 18-20hr  (627) embryo 20-22hr  (822) embryo 22-24hr  (805) larva L1  (1674) larva L2  (1537) larva L3 12hr old  (1521) larva L3 puffstage 1-2  (1356) larva L3 puffstage 3-6  (762) larva L3 puffstage 7-9  (343) white prepupae new  (355) white prepupae 12hr  (208) white prepupae 24hr  (158) pupae 2d postWPP  (309) pupae 3d postWPP  (664) pupae 4d postWPP  (628) adult male 01day  (786) adult male 05day  (543) adult male 30day  (383) adult female 01day  (1119) adult female 05day  (918) adult female 30day  (869) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    986 embryo 02-04hr    30 embryo 04-06hr    14 embryo 06-08hr    9 embryo 08-10hr    13 embryo 10-12hr    33 embryo 12-14hr    72 embryo 14-16hr    374 embryo 16-18hr    515 embryo 18-20hr    627 embryo 20-22hr    822 embryo 22-24hr    805 larva L1    1674 larva L2    1537 larva L3 12hr old    1521 larva L3 puffstage 1-2    1356 larva L3 puffstage 3-6    762 larva L3 puffstage 7-9    343 white prepupae new    355 white prepupae 12hr    208 white prepupae 24hr    158 pupae 2d postWPP    309 pupae 3d postWPP    664 pupae 4d postWPP    628 adult male 01day    786 adult male 05day    543 adult male 30day    383 adult female 01day    1119 adult female 05day    918 adult female 30day    869 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    39 Schneider line 2 Sg4    268 embryonic 1182-4H    252 embryonic GM2    398 embryonic Kc167    1062 embryonic S1    801 embryonic S3    199 leg disc CME L1    336 wing disc CME-W2    574 wing disc ML-DmD8    663 wing disc ML-DmD9    667 wing disc ML-DmD16-c3    453 wing disc ML-DmD21    285 wing disc ML-DmD32    197 haltere disc ML-DmD17-c3    361 eye-antennal disc ML-DmD11    515 antennal disc ML-DmD20-c5    295 mixed discs ML-DmD4-c1    475 CNS ML-DmBG1-c1    644 CNS ML-DmBG2-c2    431 tumorous blood cells mbn2    462 ovary fGS/OSS    498 ovary OSC    372 ovary OSS    200 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (39) Schneider line 2 Sg4  (268) embryonic 1182-4H  (252) embryonic GM2  (398) embryonic Kc167  (1062) embryonic S1  (801) embryonic S3  (199) leg disc CME L1  (336) wing disc CME-W2  (574) wing disc ML-DmD8  (663) wing disc ML-DmD9  (667) wing disc ML-DmD16-c3  (453) wing disc ML-DmD21  (285) wing disc ML-DmD32  (197) haltere disc ML-DmD17-c3  (361) eye-antennal disc ML-DmD11  (515) antennal disc ML-DmD20-c5  (295) mixed discs ML-DmD4-c1  (475) CNS ML-DmBG1-c1  (644) CNS ML-DmBG2-c2  (431) tumorous blood cells mbn2  (462) ovary fGS/OSS  (498) ovary OSC  (372) ovary OSS  (200) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    39 Schneider line 2 Sg4  (268) embryonic 1182-4H  (252) embryonic GM2  (398) embryonic Kc167  (1062) embryonic S1  (801) embryonic S3  (199) leg disc CME L1  (336) wing disc CME-W2  (574) wing disc ML-DmD8  (663) wing disc ML-DmD9  (667) wing disc ML-DmD16-c3  (453) wing disc ML-DmD21  (285) wing disc ML-DmD32  (197) haltere disc ML-DmD17-c3  (361) eye-antennal disc ML-DmD11  (515) antennal disc ML-DmD20-c5  (295) mixed discs ML-DmD4-c1  (475) CNS ML-DmBG1-c1  (644) CNS ML-DmBG2-c2  (431) tumorous blood cells mbn2  (462) ovary fGS/OSS  (498) ovary OSC  (372) ovary OSS  (200) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    39 Schneider line 2 Sg4    268 embryonic 1182-4H    252 embryonic GM2    398 embryonic Kc167    1062 embryonic S1    801 embryonic S3    199 leg disc CME L1    336 wing disc CME-W2    574 wing disc ML-DmD8    663 wing disc ML-DmD9    667 wing disc ML-DmD16-c3    453 wing disc ML-DmD21    285 wing disc ML-DmD32    197 haltere disc ML-DmD17-c3    361 eye-antennal disc ML-DmD11    515 antennal disc ML-DmD20-c5    295 mixed discs ML-DmD4-c1    475 CNS ML-DmBG1-c1    644 CNS ML-DmBG2-c2    431 tumorous blood cells mbn2    462 ovary fGS/OSS    498 ovary OSC    372 ovary OSS    200 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    39 Schneider line 2 Sg4    268 embryonic 1182-4H    252 embryonic GM2    398 embryonic Kc167    1062 embryonic S1    801 embryonic S3    199 leg disc CME L1    336 wing disc CME-W2    574 wing disc ML-DmD8    663 wing disc ML-DmD9    667 wing disc ML-DmD16-c3    453 wing disc ML-DmD21    285 wing disc ML-DmD32    197 haltere disc ML-DmD17-c3    361 eye-antennal disc ML-DmD11    515 antennal disc ML-DmD20-c5    295 mixed discs ML-DmD4-c1    475 CNS ML-DmBG1-c1    644 CNS ML-DmBG2-c2    431 tumorous blood cells mbn2    462 ovary fGS/OSS    498 ovary OSC    372 ovary OSS    200 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (39) Schneider line 2 Sg4  (268) embryonic 1182-4H  (252) embryonic GM2  (398) embryonic Kc167  (1062) embryonic S1  (801) embryonic S3  (199) leg disc CME L1  (336) wing disc CME-W2  (574) wing disc ML-DmD8  (663) wing disc ML-DmD9  (667) wing disc ML-DmD16-c3  (453) wing disc ML-DmD21  (285) wing disc ML-DmD32  (197) haltere disc ML-DmD17-c3  (361) eye-antennal disc ML-DmD11  (515) antennal disc ML-DmD20-c5  (295) mixed discs ML-DmD4-c1  (475) CNS ML-DmBG1-c1  (644) CNS ML-DmBG2-c2  (431) tumorous blood cells mbn2  (462) ovary fGS/OSS  (498) ovary OSC  (372) ovary OSS  (200) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    39 Schneider line 2 Sg4  (268) embryonic 1182-4H  (252) embryonic GM2  (398) embryonic Kc167  (1062) embryonic S1  (801) embryonic S3  (199) leg disc CME L1  (336) wing disc CME-W2  (574) wing disc ML-DmD8  (663) wing disc ML-DmD9  (667) wing disc ML-DmD16-c3  (453) wing disc ML-DmD21  (285) wing disc ML-DmD32  (197) haltere disc ML-DmD17-c3  (361) eye-antennal disc ML-DmD11  (515) antennal disc ML-DmD20-c5  (295) mixed discs ML-DmD4-c1  (475) CNS ML-DmBG1-c1  (644) CNS ML-DmBG2-c2  (431) tumorous blood cells mbn2  (462) ovary fGS/OSS  (498) ovary OSC  (372) ovary OSS  (200) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    39 Schneider line 2 Sg4    268 embryonic 1182-4H    252 embryonic GM2    398 embryonic Kc167    1062 embryonic S1    801 embryonic S3    199 leg disc CME L1    336 wing disc CME-W2    574 wing disc ML-DmD8    663 wing disc ML-DmD9    667 wing disc ML-DmD16-c3    453 wing disc ML-DmD21    285 wing disc ML-DmD32    197 haltere disc ML-DmD17-c3    361 eye-antennal disc ML-DmD11    515 antennal disc ML-DmD20-c5    295 mixed discs ML-DmD4-c1    475 CNS ML-DmBG1-c1    644 CNS ML-DmBG2-c2    431 tumorous blood cells mbn2    462 ovary fGS/OSS    498 ovary OSC    372 ovary OSS    200 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    359 cold shock, 4-day adult    249 heat shock, 4-day adult    1409 Cadmium 50 mM 6 hrs, larvae L3    1550 Cadmium 50 mM 12 hrs, larvae L3    1730 Cadmium 50 mM 48 hrs, 4-day adult    1379 Cadmium 100 mM 48 hrs, 4-day adult    856 Copper 0.5 mM 12 hrs, larvae L3    1413 Copper 15 mM 48 hrs, 4-day adult    367 Zinc 5 mM 12 hrs, larvae L3    898 Zinc 4.5 mM 48 hrs, 4-day adult    270 Ethanol 2.5% 3 hrs, larvae L3    963 Ethanol 5% 3 hrs, larvae L3    2106 Ethanol 10% 3 hrs, larvae L3    2385 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1580 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    725 Caffeine 25 mg/ml 48 hrs, 4-day adult    321 Paraquat 5 mM 48 hrs, 4-day adult    810 Paraquat 10 mM 48 hrs, 4-day adult    1022 Rotenone 2 μg 12 hrs, larvae L3    1913 Rotenone 8 μg 12 hrs, larvae L3    1851 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (359) cold shock, 4-day adult  (249) heat shock, 4-day adult  (1409) Cadmium 50 mM 6 hrs, larvae L3  (1550) Cadmium 50 mM 12 hrs, larvae L3  (1730) Cadmium 50 mM 48 hrs, 4-day adult  (1379) Cadmium 100 mM 48 hrs, 4-day adult  (856) Copper 0.5 mM 12 hrs, larvae L3  (1413) Copper 15 mM 48 hrs, 4-day adult  (367) Zinc 5 mM 12 hrs, larvae L3  (898) Zinc 4.5 mM 48 hrs, 4-day adult  (270) Ethanol 2.5% 3 hrs, larvae L3  (963) Ethanol 5% 3 hrs, larvae L3  (2106) Ethanol 10% 3 hrs, larvae L3  (2385) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (1580) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (725) Caffeine 25 mg/ml 48 hrs, 4-day adult  (321) Paraquat 5 mM 48 hrs, 4-day adult  (810) Paraquat 10 mM 48 hrs, 4-day adult  (1022) Rotenone 2 μg 12 hrs, larvae L3  (1913) Rotenone 8 μg 12 hrs, larvae L3  (1851) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (359) cold shock, 4-day adult  (249) heat shock, 4-day adult  (1409) Cadmium 50 mM 6 hrs, larvae L3  (1550) Cadmium 50 mM 12 hrs, larvae L3  (1730) Cadmium 50 mM 48 hrs, 4-day adult  (1379) Cadmium 100 mM 48 hrs, 4-day adult  (856) Copper 0.5 mM 12 hrs, larvae L3  (1413) Copper 15 mM 48 hrs, 4-day adult  (367) Zinc 5 mM 12 hrs, larvae L3  (898) Zinc 4.5 mM 48 hrs, 4-day adult  (270) Ethanol 2.5% 3 hrs, larvae L3  (963) Ethanol 5% 3 hrs, larvae L3  (2106) Ethanol 10% 3 hrs, larvae L3  (2385) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (1580) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (725) Caffeine 25 mg/ml 48 hrs, 4-day adult  (321) Paraquat 5 mM 48 hrs, 4-day adult  (810) Paraquat 10 mM 48 hrs, 4-day adult  (1022) Rotenone 2 μg 12 hrs, larvae L3  (1913) Rotenone 8 μg 12 hrs, larvae L3  (1851) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    359 cold shock, 4-day adult    249 heat shock, 4-day adult    1409 Cadmium 50 mM 6 hrs, larvae L3    1550 Cadmium 50 mM 12 hrs, larvae L3    1730 Cadmium 50 mM 48 hrs, 4-day adult    1379 Cadmium 100 mM 48 hrs, 4-day adult    856 Copper 0.5 mM 12 hrs, larvae L3    1413 Copper 15 mM 48 hrs, 4-day adult    367 Zinc 5 mM 12 hrs, larvae L3    898 Zinc 4.5 mM 48 hrs, 4-day adult    270 Ethanol 2.5% 3 hrs, larvae L3    963 Ethanol 5% 3 hrs, larvae L3    2106 Ethanol 10% 3 hrs, larvae L3    2385 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1580 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    725 Caffeine 25 mg/ml 48 hrs, 4-day adult    321 Paraquat 5 mM 48 hrs, 4-day adult    810 Paraquat 10 mM 48 hrs, 4-day adult    1022 Rotenone 2 μg 12 hrs, larvae L3    1913 Rotenone 8 μg 12 hrs, larvae L3    1851 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    359 cold shock, 4-day adult    249 heat shock, 4-day adult    1409 Cadmium 50 mM 6 hrs, larvae L3    1550 Cadmium 50 mM 12 hrs, larvae L3    1730 Cadmium 50 mM 48 hrs, 4-day adult    1379 Cadmium 100 mM 48 hrs, 4-day adult    856 Copper 0.5 mM 12 hrs, larvae L3    1413 Copper 15 mM 48 hrs, 4-day adult    367 Zinc 5 mM 12 hrs, larvae L3    898 Zinc 4.5 mM 48 hrs, 4-day adult    270 Ethanol 2.5% 3 hrs, larvae L3    963 Ethanol 5% 3 hrs, larvae L3    2106 Ethanol 10% 3 hrs, larvae L3    2385 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1580 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    725 Caffeine 25 mg/ml 48 hrs, 4-day adult    321 Paraquat 5 mM 48 hrs, 4-day adult    810 Paraquat 10 mM 48 hrs, 4-day adult    1022 Rotenone 2 μg 12 hrs, larvae L3    1913 Rotenone 8 μg 12 hrs, larvae L3    1851 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (359) cold shock, 4-day adult  (249) heat shock, 4-day adult  (1409) Cadmium 50 mM 6 hrs, larvae L3  (1550) Cadmium 50 mM 12 hrs, larvae L3  (1730) Cadmium 50 mM 48 hrs, 4-day adult  (1379) Cadmium 100 mM 48 hrs, 4-day adult  (856) Copper 0.5 mM 12 hrs, larvae L3  (1413) Copper 15 mM 48 hrs, 4-day adult  (367) Zinc 5 mM 12 hrs, larvae L3  (898) Zinc 4.5 mM 48 hrs, 4-day adult  (270) Ethanol 2.5% 3 hrs, larvae L3  (963) Ethanol 5% 3 hrs, larvae L3  (2106) Ethanol 10% 3 hrs, larvae L3  (2385) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (1580) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (725) Caffeine 25 mg/ml 48 hrs, 4-day adult  (321) Paraquat 5 mM 48 hrs, 4-day adult  (810) Paraquat 10 mM 48 hrs, 4-day adult  (1022) Rotenone 2 μg 12 hrs, larvae L3  (1913) Rotenone 8 μg 12 hrs, larvae L3  (1851) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (359) cold shock, 4-day adult  (249) heat shock, 4-day adult  (1409) Cadmium 50 mM 6 hrs, larvae L3  (1550) Cadmium 50 mM 12 hrs, larvae L3  (1730) Cadmium 50 mM 48 hrs, 4-day adult  (1379) Cadmium 100 mM 48 hrs, 4-day adult  (856) Copper 0.5 mM 12 hrs, larvae L3  (1413) Copper 15 mM 48 hrs, 4-day adult  (367) Zinc 5 mM 12 hrs, larvae L3  (898) Zinc 4.5 mM 48 hrs, 4-day adult  (270) Ethanol 2.5% 3 hrs, larvae L3  (963) Ethanol 5% 3 hrs, larvae L3  (2106) Ethanol 10% 3 hrs, larvae L3  (2385) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (1580) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (725) Caffeine 25 mg/ml 48 hrs, 4-day adult  (321) Paraquat 5 mM 48 hrs, 4-day adult  (810) Paraquat 10 mM 48 hrs, 4-day adult  (1022) Rotenone 2 μg 12 hrs, larvae L3  (1913) Rotenone 8 μg 12 hrs, larvae L3  (1851) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    359 cold shock, 4-day adult    249 heat shock, 4-day adult    1409 Cadmium 50 mM 6 hrs, larvae L3    1550 Cadmium 50 mM 12 hrs, larvae L3    1730 Cadmium 50 mM 48 hrs, 4-day adult    1379 Cadmium 100 mM 48 hrs, 4-day adult    856 Copper 0.5 mM 12 hrs, larvae L3    1413 Copper 15 mM 48 hrs, 4-day adult    367 Zinc 5 mM 12 hrs, larvae L3    898 Zinc 4.5 mM 48 hrs, 4-day adult    270 Ethanol 2.5% 3 hrs, larvae L3    963 Ethanol 5% 3 hrs, larvae L3    2106 Ethanol 10% 3 hrs, larvae L3    2385 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1580 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    725 Caffeine 25 mg/ml 48 hrs, 4-day adult    321 Paraquat 5 mM 48 hrs, 4-day adult    810 Paraquat 10 mM 48 hrs, 4-day adult    1022 Rotenone 2 μg 12 hrs, larvae L3    1913 Rotenone 8 μg 12 hrs, larvae L3    1851 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_10 mE1_20_mRNA_expression_cluster_10
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001092 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8893-RA;CG8893-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Gapdh2d08479 viable, with Scer\GAL4pnr-MD237 Gapdh2GD17541 Sterility Allele fertile Gapdh2d08479 Phenotype manifest in Allele
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of Gapdh2 Class Mutagen Stocks Known lesion Gapdh2d08479 P-element activity 2 -- Gapdh2d08145 1 -- Gapdh2GG01761 1 -- Gapdh2d08344 0 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Gapdh2 Class Mutagen Stocks Known lesion Gapdh2GD17541 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Gapdh2JF02072 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Gapdh2KK112708 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Gapdh2-0.9 in vitro construct 0 Yes Gapdh2Gapdh1.cSa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD17541} NA P{KK112708} NA P{TRiP.JF02072} NA characterization construct P{GAP1/2} NA P{GAP2-0.9} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{Mae-UAS.6.11}Gapdh2GG01761 NA P{XP}Gapdh2d08145 NA P{XP}Gapdh2d08344 NA P{XP}Gapdh2d08479 NA
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function
CV term Evidence References
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity inferred from direct assay (Sun et al., 1988)
Biological Process
CV term Evidence References
glycolysis inferred from expression pattern (Sun et al., 1988)
Cellular Component
CV term Evidence References
lipid particle inferred from direct assay (Beller et al., 2006, Beller et al., 2006) microtubule associated complex inferred from direct assay (Hughes et al., 2008)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity inferred from sequence or structural similarity with UniProtKB:P17244 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1988.4.1) NAD binding inferred from electronic annotation with InterPro:IPR006424 (FlyBase Curators et al., 2004-) NADP binding inferred from electronic annotation with InterPro:IPR006424 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
glycolysis non-traceable author statement (Swiss-Prot Project Members, 1988.4.1) oxidation-reduction process inferred from electronic annotation with InterPro:IPR006424, InterPro:IPR020828, InterPro:IPR020829, InterPro:IPR020830, InterPro:IPR020831 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
cytoplasm non-traceable author statement (Swiss-Prot Project Members, 1988.4.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Gapdh2 - Adh experimental knowledge based (Guruharsha et al., 2011) Gapdh2 - Ald experimental knowledge based (Guruharsha et al., 2011) Gapdh2 - Argk experimental knowledge based (Guruharsha et al., 2011) Gapdh2 - CG10286 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Gapdh2 - CG10581 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Gapdh2 - CG14141 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Gapdh2 - CG14262 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Gapdh2 - CG16826 experimental knowledge based (Guruharsha et al., 2011) Gapdh2 - CG17562 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Gapdh2 - CG18858 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Gapdh2 - CG2010 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Gapdh2 - CG2931 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Gapdh2 - CG31343 experimental knowledge based (Guruharsha et al., 2011) Gapdh2 - CG31522 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Gapdh2 - CG3409 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Gapdh2 - CG3731 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Gapdh2 - CG42319 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Gapdh2 - CG4730 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Gapdh2 - CG5500 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Gapdh2 - CG6330 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Gapdh2 - CG7091 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Gapdh2 - CG8005 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Gapdh2 - Eno experimental knowledge based (Guruharsha et al., 2011) Gapdh2 - Ent1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Gapdh2 - Gapdh1 experimental knowledge based (Guruharsha et al., 2011) Gapdh2 - GlyP experimental knowledge based (Guruharsha et al., 2011) Gapdh2 - Gpdh experimental knowledge based (Guruharsha et al., 2011) Gapdh2 - Hr46 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Gapdh2 - Lsp2 experimental knowledge based (Guruharsha et al., 2011) Gapdh2 - Mp20 experimental knowledge based (Guruharsha et al., 2011) Gapdh2 - Pgk experimental knowledge based (Guruharsha et al., 2011) Gapdh2 - Rfabg experimental knowledge based (Guruharsha et al., 2011) Gapdh2 - Scp1 experimental knowledge based (Guruharsha et al., 2011) Gapdh2 - Yp2 experimental knowledge based (Guruharsha et al., 2011) Gapdh2 - regucalcin anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Gapdh2 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 58893 DroID - A comprehensive database of gene and protein interactions. • FBgn0001092 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32545
Orthologs
OrthoDB Orthologs (149) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6GJ086 Dipteran inclusive ortholog search EOG67SSPK Insect inclusive ortholog search EOG670SD2 Arthropod inclusive ortholog search EOG66M91C Metazoa inclusive ortholog search EOG6BGNPV
Orthologs in Drosophila Species (EOG6GJ086)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Gapdh1     Drosophila melanogaster fruit fly  Dmel\Gapdh2     Drosophila sechellia   Dsec\GM20756   Y Drosophila sechellia   Dsec\GM22592 Y Y Drosophila erecta   Dere\GG10711   Y Drosophila erecta   Dere\GG17924 Y Y Drosophila yakuba   Dyak\Gapdh1   Y Drosophila yakuba   Dyak\GE17231 Y Y Drosophila ananassae   Dana\GF17051   Y Drosophila pseudoobscura pseudoobscura   Dpse\Gapdh2   Y Drosophila persimilis   Dper\GL10260   Y Drosophila willistoni   Dwil\GK17768 Y Y Drosophila virilis   Dvir\GJ20492   Y Drosophila mojavensis   Dmoj\GI20756   Y Drosophila grimshawi   Dgri\GH21340   Y
Orthologs in non-Drosophila Dipterans (EOG67SSPK)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL016984 Y Anopheles gambiae Malaria mosquito  Agam\AGAP009623 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ005597 Y
Orthologs in non-Dipteran Insects (EOG670SD2)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\Gapdh Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007624 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH16788 Y Atta cephalotes Leafcutter ant  Acep\ACEP17085 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO18811 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL18465 Y Linepithema humile Argentine ant  Lhum\LH21746 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB21096 Y Solenopsis invicta Red fire ant  Sinv\SINV11437 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI002318 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI008700 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI009769 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI009148 Y Bombyx mori Silkmoth  Bmor\BmGAPDH Y Pediculus humanus Human body louse  Phum\PHUM128020 Y Tribolium castaneum Red flour beetle  Tcas\TC006170 Y
Orthologs in non-Insect Arthropods (EOG66M91C)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Olr1766 Y Ixodes scapularis Deer tick  Isca\ISCW018700 Y
Orthologs in non-Arthropod Metazoa (EOG6BGNPV)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\gpd-2 Y Caenorhabditis elegans Nematode  Cele\gpd-4 Y Caenorhabditis elegans Nematode  Cele\gpd-1 Y Caenorhabditis elegans Nematode  Cele\gpd-3 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_007155gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_027041gn Y Danio rerio Zebrafish  Drer\gapdh Y Danio rerio Zebrafish  Drer\gapdhs Y Xenopus tropicalis Western clawed frog  Xtro\gapdh Y Gallus gallus Domestic chicken  Ggal\ENSGALG00000008782 Y Gallus gallus Domestic chicken  Ggal\GAPDH Y Mus musculus House mouse  Mmus\Gm7293 Y Mus musculus House mouse  Mmus\Gm3200 Y Mus musculus House mouse  Mmus\Gm3839 Y Mus musculus House mouse  Mmus\Gapdhs Y Mus musculus House mouse  Mmus\Gm10291 Y Mus musculus House mouse  Mmus\Gm7251 Y Mus musculus House mouse  Mmus\Gm5069 Y Mus musculus House mouse  Mmus\Gm10481 Y Mus musculus House mouse  Mmus\Gm6981 Y Mus musculus House mouse  Mmus\Gm3222 Y Mus musculus House mouse  Mmus\Gapdh Y Mus musculus House mouse  Mmus\Gm10290 Y Mus musculus House mouse  Mmus\Gm10293 Y Mus musculus House mouse  Mmus\Gm6669 Y Mus musculus House mouse  Mmus\Gm10313 Y Mus musculus House mouse  Mmus\Gm10566 Y Mus musculus House mouse  Mmus\Gm5507 Y Mus musculus House mouse  Mmus\Gm4795 Y Mus musculus House mouse  Mmus\Gm8055 Y Mus musculus House mouse  Mmus\Gm9034 Y Mus musculus House mouse  Mmus\ENSMUSG00000059468 Y Mus musculus House mouse  Mmus\Gm10327 Y Mus musculus House mouse  Mmus\Gm6316 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000033388 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000036820 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000029111 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000024418 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000017139 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000027574 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000023547 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000032611 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000033797 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000038964 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000031758 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000016400 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000029635 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000034205 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000015856 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000038181 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000024217 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000009394 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000026809 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000032435 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000024500 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000033702 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000032205 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000009844 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000030429 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000033506 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000042521 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000042057 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000022425 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000032069 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000030056 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000027471 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000032754 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000017388 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000024486 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000042908 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000025316 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000027866 Y Rattus norvegicus Norway rat  Rnor\Gapdhs Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000042034 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000021319 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000025756 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000008363 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000033732 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000043395 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000023260 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000031519 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000042481 Y Rattus norvegicus Norway rat  Rnor\Gapdh-ps2 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000010043 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000026991 Y Rattus norvegicus Norway rat  Rnor\RGD1564688 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000019726 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000023365 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000043263 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000034144 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000031542 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000030638 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000023960 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000037802 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000024166 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000033635 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000021858 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000022174 Y Rattus norvegicus Norway rat  Rnor\Gapdh Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000031351 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000025797 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000025201 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000029758 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000024155 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000033401 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000031761 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000042314 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000002195 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000031978 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000029967 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000042051 Y Homo sapiens Human  Hsap\GAPDH Y Homo sapiens Human  Hsap\GAPDHS Y
Human Orthologs (2)
Gene OMIM HGNC Hsap\GAPDH     Hsap\GAPDHS
AAA Orthologs (4) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila sechellia Dsec\GM22592 Drosophila erecta Dere\GG17924 Drosophila yakuba Dyak\GE17231 Drosophila willistoni Dwil\GK17768
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	19295 w1118 P{XP}Gapdh2d08479 26302 y1 v1; P{TRiP.JF02072}attP2 19967 y1 w67c23 P{Mae-UAS.6.11}Gapdh2GG01761
Harvard	- P{XP}Gapdh2d08145 - P{XP}Gapdh2d08479
VDRC	v106562 P{KK112708}VIE-260B v50351 w1118 P{GD17541}v50351
Genomic Clones ( 2 )
	BACR22H11 BACR25C18 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 478 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD24323 RE69448 RH55882
Other clones	yak-em_Gapdh1
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT05794 GH06428 GH11114 GH12769 GH13544 GH18657 GH18827 GH20106 GH20382 GH20927 GM07781 GM18040 GM23109 HL02493 HL05372 LP03016 LP04017 LP04466 LP07701 LP18119 RE04629 RE06395 RE07869 RE16627 RE28103 RE29018 RE29684 RE30314 RE31795 RE35457 RE39947 RE42060 RE56336 RE59361 RE68490 RE73185 RH02235 RH03024 RH04375 RH05364 RH06413 RH07031 RH08286 RH09778 RH09830 RH09982 RH10026 RH12121 RH13032 RH13058 RH16538 RH19033 RH19673 RH20028 RH22333 RH22428 RH25147 RH28031 RH28442 RH30054 RH32352 RH33850 RH34674 RH34951 RH37311 RH37393 RH37464 RH38327 RH39211 RH39570 RH43914 RH44508 RH46141 RH48826 RH49813 RH50128 RH50167 RH50181 RH50473 RH51896 RH52287 RH53016 RH53834 RH54176 RH54230 RH54462 RH56628 RH60596 RH61865 RH63010 RH65680 RH65918 RH66214 RH67458 RH67636 RH67941 RH68121 RH68245 RH68570 RH68824 RH69366 RH70870 RH72305 RH72511 SD01295 SD03019 SD03184 SD11285 SD11376 SD13328 SD18705
Other clones	9658 10551 30583 57660 59741 63370 71160 117658 473613 bs08e07 CK01.120B.D9 CK01.126D.G2 Dmel_dig_003033_0715_0378 Dmel_dig_004095_0685_0656 Dmel_dig_011017_0581_2392 Dmel_dig_011983_0662_2988 Dmel_dig_014506_0704_3375 Dmel_dig_016268_0637_1720 Dmel_dig_017087_0666_3172 Dmel_dig_018484_0724_0402 Dmel_dig_018486_0685_2820 Dmel_dig_020566_0680_2722 Dmel_dig_020932_0666_0909 Dmel_neb_000480_0892_3745 Dmel_neb_012461_0914_2138 Dmel_neb_013017_0104_2243 Dmel_neb_013813_0407_3444 Dmel_neb_014794_0094_3500 Dmel_neb_015450_0146_2793 EC01473 EC15073 EC22125 EC34295 EK097157 EK113619 EK139463 EK148754 EK178622 EK186351 EK189219 EN00340 ESG011a.E7.4_B10 ESG011a.E21_B10 ESG011b.E7_C02 ESG014c.E21_E10 FGC015H06 FGH129H04 FGM101A08 FGM105A06 FGM212D10 FGM222A08 GH04145 GH04172 GH04788 GH04923 GH05091 GH10435 GH12924 GH13371 GH13659 GH13743 GH15562 GH15575 GH16786 GH17903 GH18328 GH19217 GH21515 GH22581 GH23738 GH23874 GH27043 GH28803 HL01175 HL01835 HL03235 HL04551 HL05370 HL05870 IP11679 IP11779 IP11879 IP11979 IP13539 LD05186 LD21635 LD22149 LD25153 LD38458 LD41902 LD46014 LD46413 LP01392 LP04646 LP05089 LP17006 LP22432 RC0-DA0001-120700-028-D08 RC0-DA0001-130700-211-A04 RC0-DA0001-130700-215-F06 RC0-DL0001-140700-024-C03 RC0-DL0001-140700-025-G11 RC0-DL0001-140700-029-C03 RC0-DL0001-140700-214-E01 RC0-DP0007-150700-011-A01 RC0-DP0007-150700-011-A08 RE23855 RH01830 RH01905 RH02119 RH02437 RH02893 RH03994 RH05375 RH05387 RH05574 RH05711 RH06288 RH08048 RH08076 RH09672 RH11424 RH13104 RH14076 RH14094 RH14325 RH14581 RH14949 RH15049 RH16109 RH16519 RH16874 RH18372 RH19719 RH19777 RH20514 RH21567 RH22838 RH25309 RH26568 RH26810 RH26924 RH27231 RH27434 RH28610 RH30149 RH30358 RH33544 RH34407 RH36977 RH37257 RH37604 RH37757 RH37782 RH38619 RH38702 RH39018 RH39203 RH39659 RH39744 RH40422 RH41425 RH42416 RH42821 RH43515 RH43522 RH43970 RH44162 RH46002 RH46378 RH47891 RH48982 RH49634 RH49708 RH50194 RH50694 RH51035 RH51173 RH52246 RH53723 RH53996 RH54391 RH56274 RH56469 RH56852 RH57052 RH58119 RH60135 RH61345 RH62331 RH63504 RH64677 RH65744 RH67438 RH67464 RH68355 RH68452 RH70702 RH71683 RH73179 RH74369 RH74382 RP003003075 RP003003801 RP003024381 RP003025703 RP003026060 RP003026853 RP003031384 RP003035554 RP003037396 RP003040378 RP003042201 RP003042681 RP003042925 RP003062486 RP003062881 RP003068902 RP003071644 RP003077442 RP003083629 RP003085880 RP003087482 RP003088648 RP003094791 RP003098026 RP003100097 RP003101018 RP003117043 RP003120693 RP003126139 RP003126667 RP003131518 RP003141032 RP003145790 RP003145974 RP003149127 RP003150375 RP003152604 RP003154913 RP003158211 RP003163988 RP003169020 RP003171828 RP003172505 RP003174369 RP003176060 RP003187603 RP003188254 RP003193113 RP003200350 RP003201084 RP003203238 RP003204684 RP003208611 RP003212689 RP003215396 RP003217062 RP003218332 RP003223067 RP003223145 RP003223146 RP003223865 RP003227838 RP003228125 RP003231746 RP003233040 RP003234071 RP003237897 RP003245408 RP003249709 RP003250107 RP003255915 RP003259051 RP003259158 RP003266130 RP003267430 RP003267744 RP003272391 RP003274937 RP003278611 RP003283367 RP003288283 RP003289302 RP003294038 RP003296852 RP003299367 RP003303874 RP003306408 RP003317072 RP003334074 RP003336402 RP003336627 RP003336721 RP003341670 RP003343590 RP003347094 RP003358607 RP003372955 RP003376029 RP003378492 RP003379623 RP003383283 RP003384818 RP003390864 RP003393431 RP003400796 RP003419020 RP003426813 RP003431233 RP003440715 RP003442920 RP003451639 RP003451709 RP003465981 RP003466443 RP003477156 RP003477254 RP003482666 RP003489210 RP003490059 RP003493951 RP003494804 RP003495669 RP003502552 RP003516019 RP003519622 RP003521954 RP003524504 RP003526287 RP003530473 RP003532372 RP003534744 RP003538272 RP003539841 RP003541853 RP003543409 RP003546072 RP003562052 RP003562229 RP003566983 RP003570250 RP003573054 RP003575118 RP003583533 RP003592301 RP003593770 RP003605346 RP003612191 RP003613755 RP003615318 RP003621024 RP003622302 RP003622928 RP003623439 RP003626292 RP003656184 RP003662938 RP003663445 RP003664332 RP003670622 RP003678808 UUGC0906
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0001092 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32545
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Gapdh2 CG8893 (Wang, 1999.11)
Source for database merge of
Additional comments	The Gapdh1 gene may have been derived from the Gapdh2 gene by retroposition. (Dai et al., 2006)
Other Comments
	At least a substantial fraction of three glycolytic enzymes of flight muscle cells are found colocalised at the Z-discs and M-lines, glycolytic enzyme colocalisation is interdependent. Localisation of Gapdh1, Gapdh2 and Ald at the Z-discs and M-lines requires the presence of at least Gpdh at the Z-discs and M-lines. (Wojtas et al., 1997) Gene contains an RNA polymerase II complex which pauses after synthesis of a short transcript. In vivo ultraviolet crosslinking techniques demonstrate phosphorylation of the carboxy terminal domain (CTD) of the large subunit of RNA polymerase II could either regulate the transition of polymerase from a paused to an elongated complex or be a consequence of the transition from paused to elongated. (O'Brien et al., 1994) The melanogaster and takahashi subgroups of the melanogaster group have small and large subunits of Gapdh, while another 28 species (in 15 subgroups) studied were found to have only either the large or the small subunit of Gapdh. (Wojtas et al., 1992) Gapdh1 and Gapdh2 RNA expression has been studied. (Sun et al., 1988) Another gene that codes for the enzyme glyceraldehyde-3-phosphate dehydrogenase (see Gapdh1). The level of expression of Gapdh2 has been found to be regulated developmentally (Sun, Lis and Wu, 1988; Sun, Tso, Lis and Wu, 1988).
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080714 NM_001272655 RefSeq (Proteins) • NP_542445 NP_001259584 DDBJ / EMBL / GenBank • AAA28561 AAF48531 AAM11293 AAN09371 AC092401 AGB95426 AI388071 AI945061 AY094940 BI623576 M11255 Entrez Gene - A searchable database of RefSeq genes. • 32545 UniProtKB/Swiss-Prot • P07487
Other Crossreferences
	EPD - Eukarytoic Promoter Database, an annotated collection of POL II promoters • 23032 InterPro domains - A database of protein families, domains, and functional sites • Glyceraldehyde-3-phosphate dehydrogenase, type I (IPR006424) Glyceraldehyde 3-phosphate dehydrogenase, NAD(P) binding domain (IPR020828) Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain (IPR020829) Glyceraldehyde 3-phosphate dehydrogenase, active site (IPR020830) Glyceraldehyde/Erythrose phosphate dehydrogenase family (IPR020831) NAD(P)-binding domain (IPR016040)
Linkouts
	BioGRID - A database of protein and genetic interactions • 58893 DroID - A comprehensive database of gene and protein interactions. • FBgn0001092 DRSC - Results from RNAi screens. • FBgn0001092 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001092 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8893-RA;CG8893-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0001092 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32545 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32545 modMine - Data generated by the modENCODE project. • FBgn0001092
Synonyms & Secondary IDs ( 17 )
Reported As
Symbol Synonym	CG8893 (Pile et al., 2003, Fulton et al., 2001, Beller et al., 2006, Dai et al., 2006, Thornton et al., 2010, Perkins et al., 2009.3.31, Keleman et al., 2009.8.5, Hughes et al., 2008, Ling and Salvaterra, 2011, Cammarato et al., 2011, Derré et al., 2007) GA3PDH (Luque et al., 1994) GADPH (Sezgin and Eanes, 2002) GAP (Boehm et al., 2003) Gapd (Matthews and Munstermann, 1994) GAPDH (Srere and Knull, 1998, Wojtas et al., 1997, Choudhary et al., 1992, van der Bliek and Meyerowitz, 1991, Mendjan et al., 2006, van der Knaap et al., 2010, Chang et al., 2010) Gapdh (Eanes, 1994, Ahrens et al., 2007, Thomas et al., 2012) Gapdh-2 (Lis, 1998, O'Brien et al., 1994, Rasmussen and Lis, 1995, Sun et al., 1988) Gapdh2 (Beller et al., 2006, Bartolome and Charlesworth, 2006, Stuart et al., 2007, Flowers et al., 2007, Li et al., 2003, Walser et al., 2006, Dorus et al., 2006, Teleman et al., 2008, Liu and Lehmann, 2008, Bandyopadhyay et al., 2006, Curtis et al., 2007, Tennessen et al., 2011, Ling and Salvaterra, 2011) GAPDH2 (Montana and Littleton, 2006) Gapdh13F   GAPDH II (de Morais Guedes et al., 2003)
Name Synonym	G3-P dehydrogenase (White et al., 1999) GAPDH2 (Cho et al., 2005) glyceraldehyde-3-phosphate dehydrogenase (Boehm et al., 2003) glyceraldehyde 3-phosphate dehydrogenase (Tennessen et al., 2011) Glyceraldehyde-3-phosphate dehydrogenase (Ling and Salvaterra, 2011) Glyceraldehyde 3 phosphate dehydrogenase 2 (Varma and Mishra, 2011, Cammarato et al., 2011)
Secondary FlyBase IDs
References ( 82 )
Generate a list of	All references relating to this gene ( 82 )
List References by type	Research paper  ( 51 ) Supplementary material  ( 9 ) Review  ( 4 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 1 ) FlyBase analysis  ( 3 ) Curated genome annotation  ( 2 ) Protein sequence record  ( 1 ) Letter  ( 1 ) Book  ( 1 )
Recent research papers ( 5 )
	Krauchunas et al., 2012, Dev. Biol. 370(1): 125--134 Protein phosphorylation changes reveal new candidates in the regulation of egg activation and early embryogenesis in D. melanogaster. [FBrf0219342] Thomas et al., 2012, PLoS ONE 7(7): e40276 A Versatile Method for Cell-Specific Profiling of Translated mRNAs in Drosophila. [FBrf0218903] Ling and Salvaterra, 2011, PLoS ONE 6(3): e17762 Robust RT-qPCR Data Normalization: Validation and Selection of Internal Reference Genes during Post-Experimental Data Analysis. [FBrf0213272] Tennessen et al., 2011, Cell Metab. 13(2): 139--148 The Drosophila Estrogen-Related Receptor Directs a Metabolic Switch that Supports Developmental Growth. [FBrf0212927] Varma and Mishra, 2011, J. Biosci., Bangalore 36(3): 439--459 Dynamics of nuclear matrix proteome during embryonic development in Drosophila melanogaster. [FBrf0214552] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...