Symbol Dmel\ GαiSpecies D. melanogaster Name G protein α i subunit Annotation symbol CG10060 Feature type protein_coding_gene FlyBase ID FBgn0001104 Gene Model Status Current Stock availability 13 publicly
available Also Known As Gαi , Giα, G-iα65A, G-ialpha65A, Gi, Gai, Galphai , G-ia65A, DGα1, Gαι65A Chromosome (arm) 3L Recombination map Cytogenetic map 65D5-65D5 Sequence location 3L:6,965,850..6,973,144 [-]
Genomic Maps
Automatically generated summary
The gene
G protein α i subunit is referred to in FlyBase by the symbol
Dmel\Gαi (CG10060, FBgn0001104). It is a protein_coding_gene from
Drosophila melanogaster . There is experimental evidence that it has the
molecular function : protein binding; DNA binding. There is experimental evidence that it is involved in the
biological process : asymmetric protein localization involved in cell fate determination; asymmetric neuroblast division; establishment of spindle orientation; establishment of glial blood-brain barrier; cortical actin cytoskeleton organization; septate junction assembly; regulation of protein localization; asymmetric cell division; ventral cord development.
33 alleles are reported . The
phenotypes of these alleles are annotated with: RP2 motor neuron; neuroblast; embryonic neuroblast; chaeta; medial triple row; RP2sib neuron; EL neuron. It has
one annotated transcript and
one annotated polypeptide .
Protein features are: G protein alpha subunit, helical insertion; G-protein alpha subunit, group I; Guanine nucleotide binding protein (G-protein), alpha subunit. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, larval trachea, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, adult spermathecae, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1627491_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of G-ialpha65A. Gene sequence location is
3L:6965850..6973144 .
User Contributed Data External Summaries
Gene/Allele symbols may differ
from current usage Gα65A: G protein α subunit at 65A
Encodes a G protein α subunit displaying sequence
homology to mammalian Giα that inhibits adenylate cyclase
activity. The G protein is uniformly distributed in oocytes,
becomes restricted to the posterior pole of the embryo during
early cleavage and is lost during the blastoderm stage (Wolfgang et al., 1990).
Description
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FlyBase data classes (e.g. genes, references, stocks) or controlled
vocabulary terms (e.g. GO, anatomy terms).
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FB2013_03
FB2013_02
Controlled Vocabulary Terms
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FlyBase Computed Cytological Location
Experimentally Determined Cytological Location
65C-65C
(determined by in situ hybridisation)
65C-65C
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Location Left of (cM) Right of (cM) Notes Comments on Gene
Model Gene model reviewed during 5.40
Gene model reviewed during 5.50
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated Transcripts Additional Transcript Data
& Comments Reported size (kB) Comments External Data Crossreferences Annotated
Polypeptides Additional Polypeptide
Data & Comments Reported size (kDa) Comments External Data Linkouts Crossreferences
domains - A database of protein families, domains, and functional sites
DDBJ / EMBL / GenBank UniProtKB/Swiss-Prot UniProtKB/TrEMBL Mapped Features have been reorganized, please
see this article for details. Additional mapped features and mutations can
be found on GBrowse or related reports. Linkouts Crossreferences Comment: maternally deposited
Additional Descriptive Data
RT-PCR analysis shows that the
G-iα65A transcript is expressed in the adult head, third segment of the antenna and maxillary palps.
Marker for Subcellular Localization CV Term
Additional Descriptive Data
G-oα65A protein staining appears at stage S10B of oogenesis, in a few granules in the oocyte, and in dorsal anterior follicle cells. By stage S12, more protein is detected in the oocytes, and all follicle cells stain. The oocyte contains high levels of uniformly distributed granules at the end of oogenesis. The distribution of
G-oα65A protein is uniform in embryonic cleavage stages 1-2, becomes restricted to the posterior pole by cleavage stage 4, and has disappeared by the end of the blastoderm stage. The protein reappears at stage 14 in presumptive cardioblast cells, and this staining persists into larval stages. After stage 15, chordotonal organ and ring gland precursors express
G-oα65A protein. Staining is also detected in a subset of cell bodies in the ventral ganglion, and in additional CNS cells.
G-iα65A protein is expressed in the neurons and at the base and inside the olfactory sensilla of the third segment of the antenna. It co-localises with
G-sα60A and
Gα49B proteins.
Low levels of
G-oα65A protein are detected throughout the CNS in both the cortex and neuropil. Higher levels of expression are detected at the terminations of photoreceptor cells in the lamina and medulla. Some staining is also detected in the ocellar retina and the antennal glomerulae, as well as in the proximal half of the ocellar nerve. Some expression is detected in the retina.
Marker for Subcellular Localization (GO Cellular Component) inferred from direct assay
Associated Tools
Reference
Linear, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
706.325
Larval Midgut
438.1
Larval Hindgut
390.2
Larval Malpighian Tubules
271.5
Larval Fat Body
133.9
Larval Salivary Gland
278.4
Larval Trachea
508.475
Larval Carcass
283.1
Adult Head
251.1
Adult Eye
215.775
Adult Brain
221.2
Adult Thoracic-Abdominal Ganglion
333.7
Adult Crop
366.5
Adult Midgut
460.5
Adult Hindgut
219
Adult Malpighian Tubules
204.4
Adult Fat Body
232.9
Adult Salivary Gland
358
Adult Heart
169.125
Adult VirginFemale Spermatheca
361.8
Adult InseminatedFemale Spermatheca
354.4
Adult Ovary
1092.2
Adult Testis
64.1
Adult Male Accessory Gland
67.6
Adult Carcass
156.2
Expression Level Scale
None
Low
Moderate
High
Very high
Linear, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
(706.325)
Larval Midgut
438.1
Larval Hindgut
390.2
Larval Malpighian Tubules
271.5
Larval Fat Body
133.9
Larval Salivary Gland
278.4
Larval Trachea
508.475
Larval Carcass
283.1
Adult Head
251.1
Adult Eye
215.775
Adult Brain
221.2
Adult Thoracic-Abdominal Ganglion
333.7
Adult Crop
366.5
Adult Midgut
460.5
Adult Hindgut
219
Adult Malpighian Tubules
204.4
Adult Fat Body
232.9
Adult Salivary Gland
358
Adult Heart
169.125
Adult VirginFemale Spermatheca
361.8
Adult InseminatedFemale Spermatheca
354.4
Adult Ovary
(1092.2)
Adult Testis
64.1
Adult Male Accessory Gland
67.6
Adult Carcass
156.2
Expression Level Scale
None
Low
Moderate
High
Linear, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
706.325
Larval Midgut
438.1
Larval Hindgut
390.2
Larval Malpighian Tubules
271.5
Larval Fat Body
133.9
Larval Salivary Gland
278.4
Larval Trachea
508.475
Larval Carcass
283.1
Adult Head
251.1
Adult Eye
215.775
Adult Brain
221.2
Adult Thoracic-Abdominal Ganglion
333.7
Adult Crop
366.5
Adult Midgut
460.5
Adult Hindgut
219
Adult Malpighian Tubules
204.4
Adult Fat Body
232.9
Adult Salivary Gland
358
Adult Heart
169.125
Adult VirginFemale Spermatheca
361.8
Adult InseminatedFemale Spermatheca
354.4
Adult Ovary
(1092.2)
Adult Testis
64.1
Adult Male Accessory Gland
67.6
Adult Carcass
156.2
Expression Level Scale
None
Low
Moderate
High
Very high
Linear, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
706.325
Larval Midgut
438.1
Larval Hindgut
390.2
Larval Malpighian Tubules
271.5
Larval Fat Body
133.9
Larval Salivary Gland
278.4
Larval Trachea
508.475
Larval Carcass
283.1
Adult Head
251.1
Adult Eye
215.775
Adult Brain
221.2
Adult Thoracic-Abdominal Ganglion
333.7
Adult Crop
366.5
Adult Midgut
460.5
Adult Hindgut
219
Adult Malpighian Tubules
204.4
Adult Fat Body
232.9
Adult Salivary Gland
358
Adult Heart
169.125
Adult VirginFemale Spermatheca
361.8
Adult InseminatedFemale Spermatheca
354.4
Adult Ovary
1092.2
Adult Testis
64.1
Adult Male Accessory Gland
67.6
Adult Carcass
156.2
Expression Level Scale
Very high
log, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
706.325
Larval Midgut
438.1
Larval Hindgut
390.2
Larval Malpighian Tubules
271.5
Larval Fat Body
133.9
Larval Salivary Gland
278.4
Larval Trachea
508.475
Larval Carcass
283.1
Adult Head
251.1
Adult Eye
215.775
Adult Brain
221.2
Adult Thoracic-Abdominal Ganglion
333.7
Adult Crop
366.5
Adult Midgut
460.5
Adult Hindgut
219
Adult Malpighian Tubules
204.4
Adult Fat Body
232.9
Adult Salivary Gland
358
Adult Heart
169.125
Adult VirginFemale Spermatheca
361.8
Adult InseminatedFemale Spermatheca
354.4
Adult Ovary
1092.2
Adult Testis
64.1
Adult Male Accessory Gland
67.6
Adult Carcass
156.2
Expression Level Scale
None
Low
Moderate
High
Very high
log, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
706.325
Larval Midgut
438.1
Larval Hindgut
390.2
Larval Malpighian Tubules
271.5
Larval Fat Body
133.9
Larval Salivary Gland
278.4
Larval Trachea
508.475
Larval Carcass
283.1
Adult Head
251.1
Adult Eye
215.775
Adult Brain
221.2
Adult Thoracic-Abdominal Ganglion
333.7
Adult Crop
366.5
Adult Midgut
460.5
Adult Hindgut
219
Adult Malpighian Tubules
204.4
Adult Fat Body
232.9
Adult Salivary Gland
358
Adult Heart
169.125
Adult VirginFemale Spermatheca
361.8
Adult InseminatedFemale Spermatheca
354.4
Adult Ovary
(1092.2)
Adult Testis
64.1
Adult Male Accessory Gland
67.6
Adult Carcass
156.2
Expression Level Scale
None
Low
Moderate
High
log, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
706.325
Larval Midgut
438.1
Larval Hindgut
390.2
Larval Malpighian Tubules
271.5
Larval Fat Body
133.9
Larval Salivary Gland
278.4
Larval Trachea
508.475
Larval Carcass
283.1
Adult Head
251.1
Adult Eye
215.775
Adult Brain
221.2
Adult Thoracic-Abdominal Ganglion
333.7
Adult Crop
366.5
Adult Midgut
460.5
Adult Hindgut
219
Adult Malpighian Tubules
204.4
Adult Fat Body
232.9
Adult Salivary Gland
358
Adult Heart
169.125
Adult VirginFemale Spermatheca
361.8
Adult InseminatedFemale Spermatheca
354.4
Adult Ovary
1092.2
Adult Testis
64.1
Adult Male Accessory Gland
67.6
Adult Carcass
156.2
Expression Level Scale
None
Low
Moderate
High
Very high
log, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
706.325
Larval Midgut
438.1
Larval Hindgut
390.2
Larval Malpighian Tubules
271.5
Larval Fat Body
133.9
Larval Salivary Gland
278.4
Larval Trachea
508.475
Larval Carcass
283.1
Adult Head
251.1
Adult Eye
215.775
Adult Brain
221.2
Adult Thoracic-Abdominal Ganglion
333.7
Adult Crop
366.5
Adult Midgut
460.5
Adult Hindgut
219
Adult Malpighian Tubules
204.4
Adult Fat Body
232.9
Adult Salivary Gland
358
Adult Heart
169.125
Adult VirginFemale Spermatheca
361.8
Adult InseminatedFemale Spermatheca
354.4
Adult Ovary
1092.2
Adult Testis
64.1
Adult Male Accessory Gland
67.6
Adult Carcass
156.2
Expression Level Scale
None
Low
Moderate
High
Very high
Heatmap
Tissue
Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass
FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level
Tissue
Adult Expression Level
NA Head
251.1
NA Eye
215.775
NA Brain
221.2
706.325 Central Nervous System
NA
NA Thoracic-Abdominal Ganglion
333.7
NA Crop
366.5
438.1 Midgut
460.5
390.2 Hindgut
219
271.5 Malpighian Tubules
204.4
133.9 Fat Body
232.9
278.4 Salivary Gland
358
NA Heart
169.125
508.475 Trachea
NA
NA VirginFemale Spermatheca
361.8
NA InseminatedFemale Spermatheca
354.4
NA Ovary
1092.2
NA Testis
64.1
NA Male Accessory Gland
67.6
283.1 Carcass
156.2
Linear, scaled to maximum expression level
Tissue
Expression Level
imaginal disc, larvae L3 wandering
37
central nervous system, larvae L3
34
central nervous system, pupae P8
16
head, virgin 1-day female
7
head, virgin 4-day female
6
head, virgin 20-day female
8
head, mated 1-day female
6
head, mated 4-day female
7
head, mated 20-day female
9
head, mated 1-day male
11
head, mated 4-day male
11
head, mated 20-day male
14
salivary gland, larvae L3 wandering
5
salivary gland, white prepupae
14
digestive system, larvae L3 wandering
28
digestive system, 1-day adult
20
digestive system, 4-day adult
20
digestive system, 20-day adult
23
fat body, larvae L3 wandering
2
fat body, white prepupae
55
fat body, pupae P8
8
carcass, larvae L3 wandering
30
carcass, 1-day adult
8
carcass, 4-day adult
9
carcass, 20-day adult
10
ovary, virgin 4-day female
102
ovary, mated 4-day female
64
testis, mated 4-day male
7
accessory gland, mated 4-day male
5
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Moderate expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
(37)
central nervous system, larvae L3
(34)
central nervous system, pupae P8
16
head, virgin 1-day female
7
head, virgin 4-day female
6
head, virgin 20-day female
8
head, mated 1-day female
6
head, mated 4-day female
7
head, mated 20-day female
9
head, mated 1-day male
11
head, mated 4-day male
11
head, mated 20-day male
14
salivary gland, larvae L3 wandering
5
salivary gland, white prepupae
14
digestive system, larvae L3 wandering
(28)
digestive system, 1-day adult
20
digestive system, 4-day adult
20
digestive system, 20-day adult
23
fat body, larvae L3 wandering
2
fat body, white prepupae
(55)
fat body, pupae P8
8
carcass, larvae L3 wandering
(30)
carcass, 1-day adult
8
carcass, 4-day adult
9
carcass, 20-day adult
10
ovary, virgin 4-day female
(102)
ovary, mated 4-day female
(64)
testis, mated 4-day male
7
accessory gland, mated 4-day male
5
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
37
central nervous system, larvae L3
34
central nervous system, pupae P8
16
head, virgin 1-day female
7
head, virgin 4-day female
6
head, virgin 20-day female
8
head, mated 1-day female
6
head, mated 4-day female
7
head, mated 20-day female
9
head, mated 1-day male
11
head, mated 4-day male
11
head, mated 20-day male
14
salivary gland, larvae L3 wandering
5
salivary gland, white prepupae
14
digestive system, larvae L3 wandering
28
digestive system, 1-day adult
20
digestive system, 4-day adult
20
digestive system, 20-day adult
23
fat body, larvae L3 wandering
2
fat body, white prepupae
55
fat body, pupae P8
8
carcass, larvae L3 wandering
30
carcass, 1-day adult
8
carcass, 4-day adult
9
carcass, 20-day adult
10
ovary, virgin 4-day female
102
ovary, mated 4-day female
64
testis, mated 4-day male
7
accessory gland, mated 4-day male
5
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
37
central nervous system, larvae L3
34
central nervous system, pupae P8
16
head, virgin 1-day female
7
head, virgin 4-day female
6
head, virgin 20-day female
8
head, mated 1-day female
6
head, mated 4-day female
7
head, mated 20-day female
9
head, mated 1-day male
11
head, mated 4-day male
11
head, mated 20-day male
14
salivary gland, larvae L3 wandering
5
salivary gland, white prepupae
14
digestive system, larvae L3 wandering
28
digestive system, 1-day adult
20
digestive system, 4-day adult
20
digestive system, 20-day adult
23
fat body, larvae L3 wandering
2
fat body, white prepupae
55
fat body, pupae P8
8
carcass, larvae L3 wandering
30
carcass, 1-day adult
8
carcass, 4-day adult
9
carcass, 20-day adult
10
ovary, virgin 4-day female
102
ovary, mated 4-day female
64
testis, mated 4-day male
7
accessory gland, mated 4-day male
5
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Tissue
Expression Level
imaginal disc, larvae L3 wandering
37
central nervous system, larvae L3
34
central nervous system, pupae P8
16
head, virgin 1-day female
7
head, virgin 4-day female
6
head, virgin 20-day female
8
head, mated 1-day female
6
head, mated 4-day female
7
head, mated 20-day female
9
head, mated 1-day male
11
head, mated 4-day male
11
head, mated 20-day male
14
salivary gland, larvae L3 wandering
5
salivary gland, white prepupae
14
digestive system, larvae L3 wandering
28
digestive system, 1-day adult
20
digestive system, 4-day adult
20
digestive system, 20-day adult
23
fat body, larvae L3 wandering
2
fat body, white prepupae
55
fat body, pupae P8
8
carcass, larvae L3 wandering
30
carcass, 1-day adult
8
carcass, 4-day adult
9
carcass, 20-day adult
10
ovary, virgin 4-day female
102
ovary, mated 4-day female
64
testis, mated 4-day male
7
accessory gland, mated 4-day male
5
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Moderate expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
(37)
central nervous system, larvae L3
(34)
central nervous system, pupae P8
16
head, virgin 1-day female
7
head, virgin 4-day female
6
head, virgin 20-day female
8
head, mated 1-day female
6
head, mated 4-day female
7
head, mated 20-day female
9
head, mated 1-day male
11
head, mated 4-day male
11
head, mated 20-day male
14
salivary gland, larvae L3 wandering
5
salivary gland, white prepupae
14
digestive system, larvae L3 wandering
28
digestive system, 1-day adult
20
digestive system, 4-day adult
20
digestive system, 20-day adult
23
fat body, larvae L3 wandering
2
fat body, white prepupae
(55)
fat body, pupae P8
8
carcass, larvae L3 wandering
30
carcass, 1-day adult
8
carcass, 4-day adult
9
carcass, 20-day adult
10
ovary, virgin 4-day female
(102)
ovary, mated 4-day female
(64)
testis, mated 4-day male
7
accessory gland, mated 4-day male
5
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
37
central nervous system, larvae L3
34
central nervous system, pupae P8
16
head, virgin 1-day female
7
head, virgin 4-day female
6
head, virgin 20-day female
8
head, mated 1-day female
6
head, mated 4-day female
7
head, mated 20-day female
9
head, mated 1-day male
11
head, mated 4-day male
11
head, mated 20-day male
14
salivary gland, larvae L3 wandering
5
salivary gland, white prepupae
14
digestive system, larvae L3 wandering
28
digestive system, 1-day adult
20
digestive system, 4-day adult
20
digestive system, 20-day adult
23
fat body, larvae L3 wandering
2
fat body, white prepupae
55
fat body, pupae P8
8
carcass, larvae L3 wandering
30
carcass, 1-day adult
8
carcass, 4-day adult
9
carcass, 20-day adult
10
ovary, virgin 4-day female
102
ovary, mated 4-day female
64
testis, mated 4-day male
7
accessory gland, mated 4-day male
5
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
37
central nervous system, larvae L3
34
central nervous system, pupae P8
16
head, virgin 1-day female
7
head, virgin 4-day female
6
head, virgin 20-day female
8
head, mated 1-day female
6
head, mated 4-day female
7
head, mated 20-day female
9
head, mated 1-day male
11
head, mated 4-day male
11
head, mated 20-day male
14
salivary gland, larvae L3 wandering
5
salivary gland, white prepupae
14
digestive system, larvae L3 wandering
28
digestive system, 1-day adult
20
digestive system, 4-day adult
20
digestive system, 20-day adult
23
fat body, larvae L3 wandering
2
fat body, white prepupae
55
fat body, pupae P8
8
carcass, larvae L3 wandering
30
carcass, 1-day adult
8
carcass, 4-day adult
9
carcass, 20-day adult
10
ovary, virgin 4-day female
102
ovary, mated 4-day female
64
testis, mated 4-day male
7
accessory gland, mated 4-day male
5
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Tissue
Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male
Linear, scaled to maximum expression level
Developmental Stage
Expression Level
embryo 00-02hr
162
embryo 02-04hr
261
embryo 04-06hr
236
embryo 06-08hr
227
embryo 08-10hr
186
embryo 10-12hr
137
embryo 12-14hr
107
embryo 14-16hr
77
embryo 16-18hr
71
embryo 18-20hr
41
embryo 20-22hr
64
embryo 22-24hr
69
larva L1
21
larva L2
25
larva L3 12hr old
19
larva L3 puffstage 1-2
25
larva L3 puffstage 3-6
36
larva L3 puffstage 7-9
60
white prepupae new
80
white prepupae 12hr
60
white prepupae 24hr
60
pupae 2d postWPP
43
pupae 3d postWPP
25
pupae 4d postWPP
19
adult male 01day
15
adult male 05day
13
adult male 30day
13
adult female 01day
44
adult female 05day
85
adult female 30day
84
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Moderate expression
Developmental Stage
Expression Level
embryo 00-02hr
(162)
embryo 02-04hr
(261)
embryo 04-06hr
(236)
embryo 06-08hr
(227)
embryo 08-10hr
(186)
embryo 10-12hr
(137)
embryo 12-14hr
(107)
embryo 14-16hr
(77)
embryo 16-18hr
(71)
embryo 18-20hr
(41)
embryo 20-22hr
(64)
embryo 22-24hr
(69)
larva L1
21
larva L2
25
larva L3 12hr old
19
larva L3 puffstage 1-2
25
larva L3 puffstage 3-6
(36)
larva L3 puffstage 7-9
(60)
white prepupae new
(80)
white prepupae 12hr
(60)
white prepupae 24hr
(60)
pupae 2d postWPP
(43)
pupae 3d postWPP
25
pupae 4d postWPP
19
adult male 01day
15
adult male 05day
13
adult male 30day
13
adult female 01day
(44)
adult female 05day
(85)
adult female 30day
(84)
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Developmental Stage
Expression Level
embryo 00-02hr
(162)
embryo 02-04hr
(261)
embryo 04-06hr
(236)
embryo 06-08hr
(227)
embryo 08-10hr
(186)
embryo 10-12hr
(137)
embryo 12-14hr
107
embryo 14-16hr
77
embryo 16-18hr
71
embryo 18-20hr
41
embryo 20-22hr
64
embryo 22-24hr
69
larva L1
21
larva L2
25
larva L3 12hr old
19
larva L3 puffstage 1-2
25
larva L3 puffstage 3-6
36
larva L3 puffstage 7-9
60
white prepupae new
80
white prepupae 12hr
60
white prepupae 24hr
60
pupae 2d postWPP
43
pupae 3d postWPP
25
pupae 4d postWPP
19
adult male 01day
15
adult male 05day
13
adult male 30day
13
adult female 01day
44
adult female 05day
85
adult female 30day
84
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Developmental Stage
Expression Level
embryo 00-02hr
162
embryo 02-04hr
261
embryo 04-06hr
236
embryo 06-08hr
227
embryo 08-10hr
186
embryo 10-12hr
137
embryo 12-14hr
107
embryo 14-16hr
77
embryo 16-18hr
71
embryo 18-20hr
41
embryo 20-22hr
64
embryo 22-24hr
69
larva L1
21
larva L2
25
larva L3 12hr old
19
larva L3 puffstage 1-2
25
larva L3 puffstage 3-6
36
larva L3 puffstage 7-9
60
white prepupae new
80
white prepupae 12hr
60
white prepupae 24hr
60
pupae 2d postWPP
43
pupae 3d postWPP
25
pupae 4d postWPP
19
adult male 01day
15
adult male 05day
13
adult male 30day
13
adult female 01day
44
adult female 05day
85
adult female 30day
84
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Developmental Stage
Expression Level
embryo 00-02hr
162
embryo 02-04hr
261
embryo 04-06hr
236
embryo 06-08hr
227
embryo 08-10hr
186
embryo 10-12hr
137
embryo 12-14hr
107
embryo 14-16hr
77
embryo 16-18hr
71
embryo 18-20hr
41
embryo 20-22hr
64
embryo 22-24hr
69
larva L1
21
larva L2
25
larva L3 12hr old
19
larva L3 puffstage 1-2
25
larva L3 puffstage 3-6
36
larva L3 puffstage 7-9
60
white prepupae new
80
white prepupae 12hr
60
white prepupae 24hr
60
pupae 2d postWPP
43
pupae 3d postWPP
25
pupae 4d postWPP
19
adult male 01day
15
adult male 05day
13
adult male 30day
13
adult female 01day
44
adult female 05day
85
adult female 30day
84
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Moderate expression
Developmental Stage
Expression Level
embryo 00-02hr
(162)
embryo 02-04hr
(261)
embryo 04-06hr
(236)
embryo 06-08hr
(227)
embryo 08-10hr
(186)
embryo 10-12hr
(137)
embryo 12-14hr
(107)
embryo 14-16hr
(77)
embryo 16-18hr
(71)
embryo 18-20hr
(41)
embryo 20-22hr
(64)
embryo 22-24hr
(69)
larva L1
21
larva L2
25
larva L3 12hr old
19
larva L3 puffstage 1-2
25
larva L3 puffstage 3-6
(36)
larva L3 puffstage 7-9
(60)
white prepupae new
(80)
white prepupae 12hr
(60)
white prepupae 24hr
(60)
pupae 2d postWPP
(43)
pupae 3d postWPP
25
pupae 4d postWPP
19
adult male 01day
15
adult male 05day
13
adult male 30day
13
adult female 01day
(44)
adult female 05day
(85)
adult female 30day
(84)
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Developmental Stage
Expression Level
embryo 00-02hr
(162)
embryo 02-04hr
(261)
embryo 04-06hr
(236)
embryo 06-08hr
(227)
embryo 08-10hr
(186)
embryo 10-12hr
137
embryo 12-14hr
107
embryo 14-16hr
77
embryo 16-18hr
71
embryo 18-20hr
41
embryo 20-22hr
64
embryo 22-24hr
69
larva L1
21
larva L2
25
larva L3 12hr old
19
larva L3 puffstage 1-2
25
larva L3 puffstage 3-6
36
larva L3 puffstage 7-9
60
white prepupae new
80
white prepupae 12hr
60
white prepupae 24hr
60
pupae 2d postWPP
43
pupae 3d postWPP
25
pupae 4d postWPP
19
adult male 01day
15
adult male 05day
13
adult male 30day
13
adult female 01day
44
adult female 05day
85
adult female 30day
84
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Developmental Stage
Expression Level
embryo 00-02hr
162
embryo 02-04hr
261
embryo 04-06hr
236
embryo 06-08hr
227
embryo 08-10hr
186
embryo 10-12hr
137
embryo 12-14hr
107
embryo 14-16hr
77
embryo 16-18hr
71
embryo 18-20hr
41
embryo 20-22hr
64
embryo 22-24hr
69
larva L1
21
larva L2
25
larva L3 12hr old
19
larva L3 puffstage 1-2
25
larva L3 puffstage 3-6
36
larva L3 puffstage 7-9
60
white prepupae new
80
white prepupae 12hr
60
white prepupae 24hr
60
pupae 2d postWPP
43
pupae 3d postWPP
25
pupae 4d postWPP
19
adult male 01day
15
adult male 05day
13
adult male 30day
13
adult female 01day
44
adult female 05day
85
adult female 30day
84
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Developmental Stage
Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day
Linear, scaled to maximum expression level
Cell Line
Expression Level
Schneider line 2 S2R+
306
Schneider line 2 Sg4
60
embryonic 1182-4H
63
embryonic GM2
68
embryonic Kc167
48
embryonic S1
60
embryonic S3
51
leg disc CME L1
46
wing disc CME-W2
57
wing disc ML-DmD8
53
wing disc ML-DmD9
44
wing disc ML-DmD16-c3
46
wing disc ML-DmD21
44
wing disc ML-DmD32
36
haltere disc ML-DmD17-c3
65
eye-antennal disc ML-DmD11
32
antennal disc ML-DmD20-c5
46
mixed discs ML-DmD4-c1
126
CNS ML-DmBG1-c1
64
CNS ML-DmBG2-c2
64
tumorous blood cells mbn2
452
ovary fGS/OSS
40
ovary OSC
46
ovary OSS
30
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Moderate expression
Cell Line
Expression Level
Schneider line 2 S2R+
(306)
Schneider line 2 Sg4
(60)
embryonic 1182-4H
(63)
embryonic GM2
(68)
embryonic Kc167
(48)
embryonic S1
(60)
embryonic S3
(51)
leg disc CME L1
(46)
wing disc CME-W2
(57)
wing disc ML-DmD8
(53)
wing disc ML-DmD9
(44)
wing disc ML-DmD16-c3
(46)
wing disc ML-DmD21
(44)
wing disc ML-DmD32
(36)
haltere disc ML-DmD17-c3
(65)
eye-antennal disc ML-DmD11
(32)
antennal disc ML-DmD20-c5
(46)
mixed discs ML-DmD4-c1
(126)
CNS ML-DmBG1-c1
(64)
CNS ML-DmBG2-c2
(64)
tumorous blood cells mbn2
(452)
ovary fGS/OSS
(40)
ovary OSC
(46)
ovary OSS
(30)
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Cell Line
Expression Level
Schneider line 2 S2R+
(306)
Schneider line 2 Sg4
60
embryonic 1182-4H
63
embryonic GM2
68
embryonic Kc167
48
embryonic S1
60
embryonic S3
51
leg disc CME L1
46
wing disc CME-W2
57
wing disc ML-DmD8
53
wing disc ML-DmD9
44
wing disc ML-DmD16-c3
46
wing disc ML-DmD21
44
wing disc ML-DmD32
36
haltere disc ML-DmD17-c3
65
eye-antennal disc ML-DmD11
32
antennal disc ML-DmD20-c5
46
mixed discs ML-DmD4-c1
(126)
CNS ML-DmBG1-c1
64
CNS ML-DmBG2-c2
64
tumorous blood cells mbn2
(452)
ovary fGS/OSS
40
ovary OSC
46
ovary OSS
30
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Cell Line
Expression Level
Schneider line 2 S2R+
306
Schneider line 2 Sg4
60
embryonic 1182-4H
63
embryonic GM2
68
embryonic Kc167
48
embryonic S1
60
embryonic S3
51
leg disc CME L1
46
wing disc CME-W2
57
wing disc ML-DmD8
53
wing disc ML-DmD9
44
wing disc ML-DmD16-c3
46
wing disc ML-DmD21
44
wing disc ML-DmD32
36
haltere disc ML-DmD17-c3
65
eye-antennal disc ML-DmD11
32
antennal disc ML-DmD20-c5
46
mixed discs ML-DmD4-c1
126
CNS ML-DmBG1-c1
64
CNS ML-DmBG2-c2
64
tumorous blood cells mbn2
452
ovary fGS/OSS
40
ovary OSC
46
ovary OSS
30
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Cell Line
Expression Level
Schneider line 2 S2R+
306
Schneider line 2 Sg4
60
embryonic 1182-4H
63
embryonic GM2
68
embryonic Kc167
48
embryonic S1
60
embryonic S3
51
leg disc CME L1
46
wing disc CME-W2
57
wing disc ML-DmD8
53
wing disc ML-DmD9
44
wing disc ML-DmD16-c3
46
wing disc ML-DmD21
44
wing disc ML-DmD32
36
haltere disc ML-DmD17-c3
65
eye-antennal disc ML-DmD11
32
antennal disc ML-DmD20-c5
46
mixed discs ML-DmD4-c1
126
CNS ML-DmBG1-c1
64
CNS ML-DmBG2-c2
64
tumorous blood cells mbn2
452
ovary fGS/OSS
40
ovary OSC
46
ovary OSS
30
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Moderate expression
Cell Line
Expression Level
Schneider line 2 S2R+
(306)
Schneider line 2 Sg4
(60)
embryonic 1182-4H
(63)
embryonic GM2
(68)
embryonic Kc167
(48)
embryonic S1
(60)
embryonic S3
(51)
leg disc CME L1
(46)
wing disc CME-W2
(57)
wing disc ML-DmD8
(53)
wing disc ML-DmD9
(44)
wing disc ML-DmD16-c3
(46)
wing disc ML-DmD21
(44)
wing disc ML-DmD32
(36)
haltere disc ML-DmD17-c3
(65)
eye-antennal disc ML-DmD11
32
antennal disc ML-DmD20-c5
(46)
mixed discs ML-DmD4-c1
(126)
CNS ML-DmBG1-c1
(64)
CNS ML-DmBG2-c2
(64)
tumorous blood cells mbn2
(452)
ovary fGS/OSS
(40)
ovary OSC
(46)
ovary OSS
30
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Cell Line
Expression Level
Schneider line 2 S2R+
(306)
Schneider line 2 Sg4
60
embryonic 1182-4H
63
embryonic GM2
68
embryonic Kc167
48
embryonic S1
60
embryonic S3
51
leg disc CME L1
46
wing disc CME-W2
57
wing disc ML-DmD8
53
wing disc ML-DmD9
44
wing disc ML-DmD16-c3
46
wing disc ML-DmD21
44
wing disc ML-DmD32
36
haltere disc ML-DmD17-c3
65
eye-antennal disc ML-DmD11
32
antennal disc ML-DmD20-c5
46
mixed discs ML-DmD4-c1
126
CNS ML-DmBG1-c1
64
CNS ML-DmBG2-c2
64
tumorous blood cells mbn2
(452)
ovary fGS/OSS
40
ovary OSC
46
ovary OSS
30
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Cell Line
Expression Level
Schneider line 2 S2R+
306
Schneider line 2 Sg4
60
embryonic 1182-4H
63
embryonic GM2
68
embryonic Kc167
48
embryonic S1
60
embryonic S3
51
leg disc CME L1
46
wing disc CME-W2
57
wing disc ML-DmD8
53
wing disc ML-DmD9
44
wing disc ML-DmD16-c3
46
wing disc ML-DmD21
44
wing disc ML-DmD32
36
haltere disc ML-DmD17-c3
65
eye-antennal disc ML-DmD11
32
antennal disc ML-DmD20-c5
46
mixed discs ML-DmD4-c1
126
CNS ML-DmBG1-c1
64
CNS ML-DmBG2-c2
64
tumorous blood cells mbn2
452
ovary fGS/OSS
40
ovary OSC
46
ovary OSS
30
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Cell Line
Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS
Linear, scaled to maximum expression level
Treatment
Expression Level
extended cold, 4-day adult
16
cold shock, 4-day adult
10
heat shock, 4-day adult
28
Cadmium 50 mM 6 hrs, larvae L3
14
Cadmium 50 mM 12 hrs, larvae L3
23
Cadmium 50 mM 48 hrs, 4-day adult
22
Cadmium 100 mM 48 hrs, 4-day adult
50
Copper 0.5 mM 12 hrs, larvae L3
7
Copper 15 mM 48 hrs, 4-day adult
26
Zinc 5 mM 12 hrs, larvae L3
8
Zinc 4.5 mM 48 hrs, 4-day adult
14
Ethanol 2.5% 3 hrs, larvae L3
50
Ethanol 5% 3 hrs, larvae L3
16
Ethanol 10% 3 hrs, larvae L3
13
Caffeine 1.5 mg/ml 4 hrs, larvae L3
11
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
44
Caffeine 25 mg/ml 48 hrs, 4-day adult
17
Paraquat 5 mM 48 hrs, 4-day adult
43
Paraquat 10 mM 48 hrs, 4-day adult
35
Rotenone 2 μg 12 hrs, larvae L3
18
Rotenone 8 μg 12 hrs, larvae L3
12
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Linear, scaled to Moderate expression
Treatment
Expression Level
extended cold, 4-day adult
16
cold shock, 4-day adult
10
heat shock, 4-day adult
(28)
Cadmium 50 mM 6 hrs, larvae L3
14
Cadmium 50 mM 12 hrs, larvae L3
23
Cadmium 50 mM 48 hrs, 4-day adult
22
Cadmium 100 mM 48 hrs, 4-day adult
(50)
Copper 0.5 mM 12 hrs, larvae L3
7
Copper 15 mM 48 hrs, 4-day adult
26
Zinc 5 mM 12 hrs, larvae L3
8
Zinc 4.5 mM 48 hrs, 4-day adult
14
Ethanol 2.5% 3 hrs, larvae L3
(50)
Ethanol 5% 3 hrs, larvae L3
16
Ethanol 10% 3 hrs, larvae L3
13
Caffeine 1.5 mg/ml 4 hrs, larvae L3
11
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
(44)
Caffeine 25 mg/ml 48 hrs, 4-day adult
17
Paraquat 5 mM 48 hrs, 4-day adult
(43)
Paraquat 10 mM 48 hrs, 4-day adult
(35)
Rotenone 2 μg 12 hrs, larvae L3
18
Rotenone 8 μg 12 hrs, larvae L3
12
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Treatment
Expression Level
extended cold, 4-day adult
16
cold shock, 4-day adult
10
heat shock, 4-day adult
28
Cadmium 50 mM 6 hrs, larvae L3
14
Cadmium 50 mM 12 hrs, larvae L3
23
Cadmium 50 mM 48 hrs, 4-day adult
22
Cadmium 100 mM 48 hrs, 4-day adult
50
Copper 0.5 mM 12 hrs, larvae L3
7
Copper 15 mM 48 hrs, 4-day adult
26
Zinc 5 mM 12 hrs, larvae L3
8
Zinc 4.5 mM 48 hrs, 4-day adult
14
Ethanol 2.5% 3 hrs, larvae L3
50
Ethanol 5% 3 hrs, larvae L3
16
Ethanol 10% 3 hrs, larvae L3
13
Caffeine 1.5 mg/ml 4 hrs, larvae L3
11
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
44
Caffeine 25 mg/ml 48 hrs, 4-day adult
17
Paraquat 5 mM 48 hrs, 4-day adult
43
Paraquat 10 mM 48 hrs, 4-day adult
35
Rotenone 2 μg 12 hrs, larvae L3
18
Rotenone 8 μg 12 hrs, larvae L3
12
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Treatment
Expression Level
extended cold, 4-day adult
16
cold shock, 4-day adult
10
heat shock, 4-day adult
28
Cadmium 50 mM 6 hrs, larvae L3
14
Cadmium 50 mM 12 hrs, larvae L3
23
Cadmium 50 mM 48 hrs, 4-day adult
22
Cadmium 100 mM 48 hrs, 4-day adult
50
Copper 0.5 mM 12 hrs, larvae L3
7
Copper 15 mM 48 hrs, 4-day adult
26
Zinc 5 mM 12 hrs, larvae L3
8
Zinc 4.5 mM 48 hrs, 4-day adult
14
Ethanol 2.5% 3 hrs, larvae L3
50
Ethanol 5% 3 hrs, larvae L3
16
Ethanol 10% 3 hrs, larvae L3
13
Caffeine 1.5 mg/ml 4 hrs, larvae L3
11
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
44
Caffeine 25 mg/ml 48 hrs, 4-day adult
17
Paraquat 5 mM 48 hrs, 4-day adult
43
Paraquat 10 mM 48 hrs, 4-day adult
35
Rotenone 2 μg 12 hrs, larvae L3
18
Rotenone 8 μg 12 hrs, larvae L3
12
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Treatment
Expression Level
extended cold, 4-day adult
16
cold shock, 4-day adult
10
heat shock, 4-day adult
28
Cadmium 50 mM 6 hrs, larvae L3
14
Cadmium 50 mM 12 hrs, larvae L3
23
Cadmium 50 mM 48 hrs, 4-day adult
22
Cadmium 100 mM 48 hrs, 4-day adult
50
Copper 0.5 mM 12 hrs, larvae L3
7
Copper 15 mM 48 hrs, 4-day adult
26
Zinc 5 mM 12 hrs, larvae L3
8
Zinc 4.5 mM 48 hrs, 4-day adult
14
Ethanol 2.5% 3 hrs, larvae L3
50
Ethanol 5% 3 hrs, larvae L3
16
Ethanol 10% 3 hrs, larvae L3
13
Caffeine 1.5 mg/ml 4 hrs, larvae L3
11
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
44
Caffeine 25 mg/ml 48 hrs, 4-day adult
17
Paraquat 5 mM 48 hrs, 4-day adult
43
Paraquat 10 mM 48 hrs, 4-day adult
35
Rotenone 2 μg 12 hrs, larvae L3
18
Rotenone 8 μg 12 hrs, larvae L3
12
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
log, scaled to Moderate expression
Treatment
Expression Level
extended cold, 4-day adult
16
cold shock, 4-day adult
10
heat shock, 4-day adult
28
Cadmium 50 mM 6 hrs, larvae L3
14
Cadmium 50 mM 12 hrs, larvae L3
23
Cadmium 50 mM 48 hrs, 4-day adult
22
Cadmium 100 mM 48 hrs, 4-day adult
(50)
Copper 0.5 mM 12 hrs, larvae L3
7
Copper 15 mM 48 hrs, 4-day adult
26
Zinc 5 mM 12 hrs, larvae L3
8
Zinc 4.5 mM 48 hrs, 4-day adult
14
Ethanol 2.5% 3 hrs, larvae L3
(50)
Ethanol 5% 3 hrs, larvae L3
16
Ethanol 10% 3 hrs, larvae L3
13
Caffeine 1.5 mg/ml 4 hrs, larvae L3
11
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
(44)
Caffeine 25 mg/ml 48 hrs, 4-day adult
17
Paraquat 5 mM 48 hrs, 4-day adult
(43)
Paraquat 10 mM 48 hrs, 4-day adult
(35)
Rotenone 2 μg 12 hrs, larvae L3
18
Rotenone 8 μg 12 hrs, larvae L3
12
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Treatment
Expression Level
extended cold, 4-day adult
16
cold shock, 4-day adult
10
heat shock, 4-day adult
28
Cadmium 50 mM 6 hrs, larvae L3
14
Cadmium 50 mM 12 hrs, larvae L3
23
Cadmium 50 mM 48 hrs, 4-day adult
22
Cadmium 100 mM 48 hrs, 4-day adult
50
Copper 0.5 mM 12 hrs, larvae L3
7
Copper 15 mM 48 hrs, 4-day adult
26
Zinc 5 mM 12 hrs, larvae L3
8
Zinc 4.5 mM 48 hrs, 4-day adult
14
Ethanol 2.5% 3 hrs, larvae L3
50
Ethanol 5% 3 hrs, larvae L3
16
Ethanol 10% 3 hrs, larvae L3
13
Caffeine 1.5 mg/ml 4 hrs, larvae L3
11
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
44
Caffeine 25 mg/ml 48 hrs, 4-day adult
17
Paraquat 5 mM 48 hrs, 4-day adult
43
Paraquat 10 mM 48 hrs, 4-day adult
35
Rotenone 2 μg 12 hrs, larvae L3
18
Rotenone 8 μg 12 hrs, larvae L3
12
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Treatment
Expression Level
extended cold, 4-day adult
16
cold shock, 4-day adult
10
heat shock, 4-day adult
28
Cadmium 50 mM 6 hrs, larvae L3
14
Cadmium 50 mM 12 hrs, larvae L3
23
Cadmium 50 mM 48 hrs, 4-day adult
22
Cadmium 100 mM 48 hrs, 4-day adult
50
Copper 0.5 mM 12 hrs, larvae L3
7
Copper 15 mM 48 hrs, 4-day adult
26
Zinc 5 mM 12 hrs, larvae L3
8
Zinc 4.5 mM 48 hrs, 4-day adult
14
Ethanol 2.5% 3 hrs, larvae L3
50
Ethanol 5% 3 hrs, larvae L3
16
Ethanol 10% 3 hrs, larvae L3
13
Caffeine 1.5 mg/ml 4 hrs, larvae L3
11
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
44
Caffeine 25 mg/ml 48 hrs, 4-day adult
17
Paraquat 5 mM 48 hrs, 4-day adult
43
Paraquat 10 mM 48 hrs, 4-day adult
35
Rotenone 2 μg 12 hrs, larvae L3
18
Rotenone 8 μg 12 hrs, larvae L3
12
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Treatment
Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3
A cluster of genes with similar mRNA expression dynamics across development.
Linkouts
- Cell culture data for RNAi and other high-throughput technologies
- Adult expression by tissue, using Affymetrix Dros2 array
mitotic domain 9 & epithelial cell
spindle & external sensory organ precursor cell I
For All Classical Alleles Show For All Alleles Carried on Transgenic
Constructs Show Disrupted in Partially disrupted in Transgenic Constructs Insertions insertion of mobile activating element
insertion of enhancer trap binary system
Molecular Function inferred from direct assay
inferred from physical interaction with
loco inferred from physical interaction with
raps Biological Process inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
Cellular Component inferred from direct assay
Molecular Function inferred from biological aspect of ancestor with PANTHER:PTN000026392
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000026392
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000026392
(assigned by RefGenome)
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000026392
(assigned by RefGenome)
Biological Process inferred from biological aspect of ancestor with PANTHER:PTN000026392
(assigned by RefGenome)
non-traceable author statement
Cellular Component traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000026392
(assigned by RefGenome)
inferred from biological aspect of ancestor with PANTHER:PTN000026392
(assigned by RefGenome)
non-traceable author statement
protein-protein anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
Interacts with Please look at the allele data
for full details of the genetic interactions
Linkouts
- A database of protein and genetic interactions
- DPiM, Drosophila Protein interaction Map
- A comprehensive database of gene and protein interactions.
Protein-protein interactions (PPI) from both known and predicted PPI data sets.
OrthoDB Ortholog Groups
OrthoDB orthology group searches
Drosophila inclusive ortholog search
Dipteran inclusive ortholog search
Insect inclusive ortholog search
Arthropod inclusive ortholog search
Metazoa inclusive ortholog search
EOG6FR1BM )
Drosophila melanogaster
fruit fly
Drosophila pseudoobscura pseudoobscura
Y
EOG6SF9FM )
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
EOG6J6QMG )
Apis mellifera
Western honey bee
Amel\LOC411704
Y
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG037232
Y
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG031438
Y
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG019514
Y
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG017607
Y
Acromyrmex echinatior
Panamanian leafcutter ant
Aech\AECH14539
Y
Acromyrmex echinatior
Panamanian leafcutter ant
Aech\AECH12191
Y
Atta cephalotes
Leafcutter ant
Acep\ACEP18018
Y
Atta cephalotes
Leafcutter ant
Acep\ACEP10660
Y
Camponotus floridanus
Florida carpenter ant
Cflo\CFLO18126
Y
Camponotus floridanus
Florida carpenter ant
Cflo\CFLO21977
Y
Harpegnathos saltator
Jerdons jumping ant
Hsal\HSAL13552
Y
Harpegnathos saltator
Jerdons jumping ant
Hsal\HSAL15055
Y
Linepithema humile
Argentine ant
Lhum\LH24762
Y
Linepithema humile
Argentine ant
Lhum\LH21928
Y
Pogonomyrmex barbatus
Red harvester ant
Pbar\PB19876
Y
Pogonomyrmex barbatus
Red harvester ant
Pbar\PB24405
Y
Solenopsis invicta
Red fire ant
Sinv\SINV18421
Y
Solenopsis invicta
Red fire ant
Sinv\SINV26210
Y
Acyrthosiphon pisum
Pea aphid
Y
Acyrthosiphon pisum
Pea aphid
Y
Pediculus humanus
Human body louse
Y
Pediculus humanus
Human body louse
Y
Tribolium castaneum
Red flour beetle
Y
Tribolium castaneum
Red flour beetle
Y
EOG6R229D )
Daphnia pulex
Water flea
Y
Daphnia pulex
Water flea
Y
Daphnia pulex
Water flea
Y
Ixodes scapularis
Deer tick
Y
EOG6S528Z )
Caenorhabditis elegans
Nematode
Y
Caenorhabditis elegans
Nematode
Y
Caenorhabditis elegans
Nematode
Y
Caenorhabditis elegans
Nematode
Y
Caenorhabditis elegans
Nematode
Y
Caenorhabditis elegans
Nematode
Y
Strongylocentrotus purpuratus
Purple sea urchin
Y
Strongylocentrotus purpuratus
Purple sea urchin
Y
Strongylocentrotus purpuratus
Purple sea urchin
Y
Xenopus tropicalis
Western clawed frog
Y
Xenopus tropicalis
Western clawed frog
Y
Xenopus tropicalis
Western clawed frog
Y
Xenopus tropicalis
Western clawed frog
Y
Xenopus tropicalis
Western clawed frog
Y
Xenopus tropicalis
Western clawed frog
Y
Xenopus tropicalis
Western clawed frog
Y
Gallus gallus
Domestic chicken
Y
Gallus gallus
Domestic chicken
Y
Gallus gallus
Domestic chicken
Y
Gallus gallus
Domestic chicken
Y
Gallus gallus
Domestic chicken
Y
Gallus gallus
Domestic chicken
Y
Mus musculus
House mouse
Y
Mus musculus
House mouse
Y
Mus musculus
House mouse
Y
Mus musculus
House mouse
Y
Mus musculus
House mouse
Y
Mus musculus
House mouse
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
AAA Orthologs (10)
based on analysis using Dmel annotation version 4.3
Drosophila pseudoobscura pseudoobscura
Bloomington Harvard Kyoto VDRC Please Note FlyBase no
longer curates genomic clone accessions so this list
may not be complete
Please Note This section lists
cDNAs and ESTs that fall within the genomic extent
of the gene model, which may include cDNAs and ESTs
of genes within introns, or of overlapping genes.
Please see
GBrowse for alignment of the cDNAs and ESTs
to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones Other clones cDNA Clones, End Sequenced
(ESTs) BDGP DGC clones Other clones Linkouts
- Results from RNAi screens.
- GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
Source for database identity of Source for identity of: G-oα65A CG10060
Source for identity of: Gαi G-iα65A
Source for database merge of Additional comments FlyBase curator comment: Genes encoding Gα subunits that have been named for their molecular function/orthology have had their symbols and names standardized following the format used in
FBrf0220456 , which also reflects popular usage in the Drosophila literature.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
dsRNA made from templates generated with primers against this gene tested in a cl-8 cell reporter assay to examine any role of
G-iα65A in the
hh signalling pathway.
Overexpression of
G-iα65A results in defects in both spindle orientation and determinant localisation in neuroblasts and sensory organ precursor cells.
Dominant adult phenotypes can be caused by the overexpression of activated Gα proteins.
Posterior localization of the
G-iα65A protein during early embryogenesis requires a subset of the posterior group genes.
The expression of
G-iα65A during development has been studied.
G-iα65A protein expression in the adult Drosophila central nervous system has been studied.
Isolated from a genomic library using bovine rod and cone transducin α subunit cDNAs as probe.
G-iα65A has been cloned and sequenced.
G proteins belong to a family of membrane-associated guanine nucleotide-binding proteins that couple specific receptors for extracellular signals to specific intracellular effectors, thus regulating the activity of these effectors. When not interacting with the receptor, G proteins are usually in the form of a heterotrimer made up of α, β and γ subunits, with the α subunit bound to GDP. Upon activation by the receptor, the α subunit exchanges GDP for GTP, dissociates from the β-γ subunits and interacts with the effector. Afterwards GTP is hydrolyzed and the heterotrimer of α, β and γ subunits is formed again.
Encodes a G protein α subunit displaying sequence homology to mammalian Giα that inhibits adenylate cyclase activity. The G protein is uniformly distributed in oocytes, becomes restricted to the posterior pole of the embryo during early cleavage and is lost during the blastoderm stage (Wolfgang et al., 1990).
Sequence Crossreferences
- A searchable database of RefSeq genes.
Other Crossreferences
domains - A database of protein families, domains, and functional sites
- Patterns of gene expression in Drosophila embryogenesis
Linkouts
- A database of protein and genetic interactions
- DPiM, Drosophila Protein interaction Map
- A comprehensive database of gene and protein interactions.
- Results from RNAi screens.
- Cell culture data for RNAi and other high-throughput technologies
- Adult expression by tissue, using Affymetrix Dros2 array
- Integrated genomics database for Drosophila, Anopheles, and C.elegans
- GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
- A cyberspace guide to Drosophila development and metazoan evolution
Protein-protein interactions (PPI) from both known and predicted PPI data sets.
- Data generated by the modENCODE project.
Reported As Symbol Synonym Giα
(
Wolfgang and Forte, 1995 ,
Horgan et al., 1995 ,
Wolfgang et al., 1991 ,
Wolfgang et al., 1991 ,
Wolfgang et al., 1990 ,
Bredendiek et al., 2011 ,
Koon and Budnik, 2012 )
G-iα65A
(
Kaltenbach et al., 2007 ,
Yi et al., 2008 ,
Stuart et al., 2007 ,
Ogden et al., 2008 ,
Yang et al., 2004 ,
Kim et al., 2005 )
Gαi
(
Yu et al., 2003 ,
Skeath and Thor, 2003 ,
Gonczy, 2002 ,
Li et al., 2000 ,
Ogden et al., 2008 ,
Yao and Carlson, 2010 ,
Cheng et al., 2012 )
Gαi
(
Yu et al., 2005 ,
Izumi et al., 2004 ,
Hampoelz and Knoblich, 2004 ,
Yu et al., 2003 ,
Betschinger and Knoblich, 2004 ,
Roegiers and Jan, 2004 ,
Fuse and Hirose, 2005 ,
David et al., 2005 ,
Lai and Orgogozo, 2004 ,
Bardin et al., 2004 ,
Siegrist and Doe, 2004 ,
Hidalgo and ffrench-Constant, 2003 ,
Wodarz and Huttner, 2003 ,
Albertson and Doe, 2003 ,
Fuse et al., 2003 ,
Tuxworth and Chia, 2003 ,
Ahringer, 2003 ,
Cai et al., 2003 ,
Chia and Yang, 2002 ,
Berdnik and Knoblich, 2002 ,
Wodarz, 2001 ,
Schaefer et al., 2001 ,
Knust, 2001 ,
Parmentier et al., 2000 ,
Greig and O'Kane, 1999.10.21 ,
Granderath et al., 1999 ,
Mistry et al., 1995 ,
Roberts et al., 1993 ,
Roberts et al., 1993 ,
Fuse and Hirose, 2006 ,
Boyle and Berg, 2006 ,
Ahringer, 2005 ,
Siegrist and Doe, 2005 ,
Schwabe et al., 2005 ,
Lee et al., 2006 ,
Krahn and Wodarz, 2008 ,
Hutterer et al., 2006 ,
Wang et al., 2005 ,
Hampoelz et al., 2005 ,
Bonaccorsi et al., 2007 ,
Lin, 2008 ,
David et al., 2005 ,
Lin et al., 2011 ,
Johnston et al., 2009 ,
Cheng et al., 2012 ,
Nipper et al., 2007 ,
Bowman et al., 2006 ,
Siller et al., 2006 ,
Izumi et al., 2006 ,
Speicher et al., 2008 ,
Katanayeva et al., 2010 ,
Boyle et al., 2010 ,
Smith and Prehoda, 2011 ,
Deng et al., 2011 ,
Yoshiura et al., 2012 )
Name Synonym G protein I a-subunit 65A
G protein α o subunit 65A
Secondary FlyBase
IDs Generate a list of List References by type
Drosophila G-protein-coupled receptor kinase 2 regulates cAMP-dependent Hedgehog signaling. [FBrf0216860] Inhibitory control of synaptic and behavioral plasticity by octopaminergic signaling. [FBrf0218256] Inscuteable regulates the Pins-Mud spindle orientation pathway. [FBrf0218437] Network organization of the huntingtin proteomic interactome in Mammalian brain. [FBrf0218881] Tre1 GPCR Signaling Orients Stem Cell Divisions in the Drosophila Central Nervous System. [FBrf0217269] Go α is involved in sugar perception in Drosophila. [FBrf0212580] Asymmetric cortical extension shifts cleavage furrow position in Drosophila neuroblasts. [FBrf0216801] The Stimulatory Gα(s) Protein Is Involved in Olfactory Signal Transduction in Drosophila. [FBrf0213437] Go signaling in mushroom bodies regulates sleep in Drosophila. [FBrf0213134] Loco signaling pathway in longevity. [FBrf0214455] Robust spindle alignment in Drosophila neuroblasts by ultrasensitive activation of pins. [FBrf0214708]
All reviews listed in FlyBase were published before 2011 
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Summary Information 
Stages(s) 1-3
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16