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General Information
Symbol
Dmel\Gαi
Species
D. melanogaster
Name
G protein α i subunit
Annotation Symbol
CG10060
Feature Type
FlyBase ID
FBgn0001104
Gene Model Status
Stock Availability
Gene Snapshot
Also Known As

G-iα65A, Gαi, G-ialpha65A, Giα, Gai

Key Links
Genomic Location
Cytogenetic map
Sequence location
3L:6,972,750..6,980,044 [-]
Recombination map

3-18

RefSeq locus
NT_037436 REGION:6972750..6980044
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (23 terms)
Molecular Function (6 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from physical interaction with UniProtKB:Q9VCX1
(assigned by UniProt )
inferred from physical interaction with FLYBASE:loco; FB:FBgn0020278
inferred from physical interaction with FLYBASE:pins; FB:FBgn0040080
inferred from physical interaction with UniProtKB:Q9W358
(assigned by UniProt )
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000026392
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000026392
(assigned by GO_Central )
inferred from electronic annotation with InterPro:IPR001408
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN000026392
(assigned by GO_Central )
Biological Process (13 terms)
Terms Based on Experimental Evidence (12 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000026392
(assigned by GO_Central )
Cellular Component (4 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from high throughput direct assay
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000026392
(assigned by GO_Central )
Protein Family (UniProt)
Belongs to the G-alpha family. G(i/o/t/z) subfamily. (P20353)
Summaries
Gene Group (FlyBase)
HETEROTRIMERIC G-PROTEIN ALPHA SUBUNITS -
The Heterotrimeric G-protein alpha subunits (Gα) can bind guanine nucleotide. When inactive, Gα is bound to GDP and associates with Gβγ forming an heterotrimer. Upon GTP binding, Gα dissociates from the heterotrimer Gαβγ. Gα has an intrinsic guanosine triphosphatase (GTPase) activity. (Adapted from FBrf0209904 and FBrf0183798).
Protein Function (UniProtKB)
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Plays a role in glial cell differentiation during embryogenesis; loco, Galphao and the G-protein coupled receptor, moody, are required in the surface glia to achieve effective insulation of the nerve cord.
(UniProt, P20353)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Gα65A: G protein α subunit at 65A
Encodes a G protein α subunit displaying sequence homology to mammalian Giα that inhibits adenylate cyclase activity. The G protein is uniformly distributed in oocytes, becomes restricted to the posterior pole of the embryo during early cleavage and is lost during the blastoderm stage (Wolfgang et al., 1990).
Summary (Interactive Fly)

G protein α subunit involved in regulating asymmetric cell division - Ric-8 regulates Gαi cortical localization to promote Gαi-dependent planar orientation of the mitotic spindle during asymmetric cell division

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\Gαi or the JBrowse view of Dmel\Gαi for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.40

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0076934
2067
355
Additional Transcript Data and Comments
Reported size (kB)

2.3, 1.7 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0076643
40.6
355
7.73
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts (via GDP- or GTP-bound forms) with loco (via GoLoco and RGS domains). Interacts with raps/pins.

(UniProt, P20353)
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Gαi using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
radioisotope in situ
Stage
Tissue/Position (including subcellular localization)
Reference
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

RT-PCR analysis shows that the G-iα65A transcript is expressed in the adult head, third segment of the antenna and maxillary palps.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

G-iα65A protein is expressed in the neurons and at the base and inside the olfactory sensilla of the third segment of the antenna. It co-localises with G-sα60A and Gα49B proteins.

G-oα65A protein staining appears at stage S10B of oogenesis, in a few granules in the oocyte, and in dorsal anterior follicle cells. By stage S12, more protein is detected in the oocytes, and all follicle cells stain. The oocyte contains high levels of uniformly distributed granules at the end of oogenesis. The distribution of G-oα65A protein is uniform in embryonic cleavage stages 1-2, becomes restricted to the posterior pole by cleavage stage 4, and has disappeared by the end of the blastoderm stage. The protein reappears at stage 14 in presumptive cardioblast cells, and this staining persists into larval stages. After stage 15, chordotonal organ and ring gland precursors express G-oα65A protein. Staining is also detected in a subset of cell bodies in the ventral ganglion, and in additional CNS cells.

Low levels of G-oα65A protein are detected throughout the CNS in both the cortex and neuropil. Higher levels of expression are detected at the terminations of photoreceptor cells in the lamina and medulla. Some staining is also detected in the ocellar retina and the antennal glomerulae, as well as in the proximal half of the ocellar nerve. Some expression is detected in the retina.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from high throughput direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Gαi in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 20 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 14 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Gαi
Transgenic constructs containing regulatory region of Gαi
Deletions and Duplications ( 3 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
cell cortex & cortical cytoskeleton, with Scer\GAL4repo
embryonic neuroblast & spindle, with Scer\GAL4VP16.mat.αTub67C
lateral cord surface glia & nucleus, with Scer\GAL4repo
mitotic domain 9 & epithelial cell
sensory mother cell & spindle, with Scer\GAL4sca-109-68
spindle & external sensory organ precursor cell I
spindle & neuroblast
spindle pole centrosome & astral microtubule, with Scer\GAL4sca-P309
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (18)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
12 of 15
Yes
Yes
11 of 15
No
Yes
8 of 15
No
Yes
8 of 15
No
Yes
6 of 15
No
Yes
5 of 15
No
No
1  
5 of 15
No
Yes
4 of 15
No
No
4 of 15
No
No
1  
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
3 of 15
No
No
1  
3 of 15
No
No
2  
2 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1  
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (16)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
12 of 15
No
Yes
11 of 15
No
Yes
8 of 15
No
Yes
8 of 15
No
Yes
6 of 15
No
Yes
5 of 15
No
Yes
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
Rattus norvegicus (Norway rat) (16)
10 of 13
Yes
Yes
10 of 13
Yes
Yes
9 of 13
No
Yes
8 of 13
No
Yes
8 of 13
No
Yes
6 of 13
No
Yes
5 of 13
No
Yes
4 of 13
No
No
4 of 13
No
No
4 of 13
No
No
4 of 13
No
No
4 of 13
No
No
3 of 13
No
No
3 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (14)
9 of 12
Yes
Yes
7 of 12
No
Yes
6 of 12
No
Yes
5 of 12
No
Yes
4 of 12
No
No
4 of 12
No
No
4 of 12
No
No
4 of 12
No
Yes
3 of 12
No
No
3 of 12
No
No
3 of 12
No
No
3 of 12
No
No
3 of 12
No
Yes
2 of 12
No
No
Danio rerio (Zebrafish) (29)
13 of 15
Yes
Yes
11 of 15
No
Yes
11 of 15
No
Yes
10 of 15
No
Yes
8 of 15
No
Yes
7 of 15
No
Yes
6 of 15
No
Yes
5 of 15
No
Yes
5 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
No
4 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
Yes
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (21)
13 of 15
Yes
Yes
8 of 15
No
Yes
6 of 15
No
Yes
5 of 15
No
No
4 of 15
No
Yes
4 of 15
No
Yes
4 of 15
No
No
4 of 15
No
Yes
4 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (4)
8 of 9
Yes
Yes
3 of 9
No
Yes
3 of 9
No
Yes
3 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (2)
7 of 15
Yes
No
7 of 15
Yes
No
Schizosaccharomyces pombe (Fission yeast) (2)
8 of 12
Yes
No
6 of 12
No
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190AAD )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915067R )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0CGF )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0CE7 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0VUT )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (7)
7 of 10
6 of 10
6 of 10
5 of 10
5 of 10
4 of 10
4 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 3 )
    Modifiers Based on Experimental Evidence ( 2 )
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts (via GDP- or GTP-bound forms) with loco (via GoLoco and RGS domains). Interacts with raps/pins.
    (UniProt, P20353 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3L
    Recombination map

    3-18

    Cytogenetic map
    Sequence location
    3L:6,972,750..6,980,044 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    65D5-65D5
    Limits computationally determined from genome sequence between P{lacW}l(3)L4060L4060 and P{PZ}corn04202
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    65C-65C
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (17)
    Genomic Clones (13)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (131)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: G-oα65A CG10060

    Source for identity of: Gαi G-iα65A

    Source for database merge of
    Additional comments

    FlyBase curator comment: Genes encoding Gα subunits that have been named for their molecular function/orthology have had their symbols and names standardized following the format used in FBrf0220456, which also reflects popular usage in the Drosophila literature.

    Other Comments

    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

    dsRNA made from templates generated with primers against this gene tested in a cl-8 cell reporter assay to examine any role of G-iα65A in the hh signalling pathway.

    Overexpression of G-iα65A results in defects in both spindle orientation and determinant localisation in neuroblasts and sensory organ precursor cells.

    Dominant adult phenotypes can be caused by the overexpression of activated Gα proteins.

    Posterior localization of the G-iα65A protein during early embryogenesis requires a subset of the posterior group genes.

    The expression of G-iα65A during development has been studied.

    G-iα65A protein expression in the adult Drosophila central nervous system has been studied.

    Isolated from a genomic library using bovine rod and cone transducin α subunit cDNAs as probe.

    G-iα65A has been cloned and sequenced.

    G proteins belong to a family of membrane-associated guanine nucleotide-binding proteins that couple specific receptors for extracellular signals to specific intracellular effectors, thus regulating the activity of these effectors. When not interacting with the receptor, G proteins are usually in the form of a heterotrimer made up of α, β and γ subunits, with the α subunit bound to GDP. Upon activation by the receptor, the α subunit exchanges GDP for GTP, dissociates from the β-γ subunits and interacts with the effector. Afterwards GTP is hydrolyzed and the heterotrimer of α, β and γ subunits is formed again.

    Encodes a G protein α subunit displaying sequence homology to mammalian Giα that inhibits adenylate cyclase activity. The G protein is uniformly distributed in oocytes, becomes restricted to the posterior pole of the embryo during early cleavage and is lost during the blastoderm stage (Wolfgang et al., 1990).

    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 50 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DPiM - Drosophila Protein interaction map
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (40)
    Reported As
    Symbol Synonym
    G-oα65A
    G-α-65A
    Galpha65A
    Gαi
    (Carmena, 2018, Richardson and Portela, 2017, Yasugi and Nishimura, 2016, Besson et al., 2015, Keder et al., 2015, Kim et al., 2015, Schweisguth, 2015, Mauri et al., 2014, Bergstralh et al., 2013, Chai et al., 2013, Chen and Jiang, 2013, Majeed et al., 2013, Cheng et al., 2012, Homem and Knoblich, 2012, Hong et al., 2012, Kelsom and Lu, 2012, Saini and Reichert, 2012, Yoshiura et al., 2012, Deng et al., 2011, Lin et al., 2011, Smith and Prehoda, 2011, Boyle et al., 2010, Katanayeva et al., 2010, Johnston et al., 2009, Kopein and Katanaev, 2009, Krahn and Wodarz, 2008, Lin, 2008, Speicher et al., 2008, Bonaccorsi et al., 2007, Nipper et al., 2007, Bowman et al., 2006, Boyle and Berg, 2006, Fuse and Hirose, 2006, Hutterer et al., 2006, Izumi et al., 2006, Lee et al., 2006, Siller et al., 2006, Ahringer, 2005, David et al., 2005, David et al., 2005, Fuse and Hirose, 2005, Hampoelz et al., 2005, Schwabe et al., 2005, Siegrist and Doe, 2005, Wang et al., 2005, Yu et al., 2005, Bardin et al., 2004, Betschinger and Knoblich, 2004, Hampoelz and Knoblich, 2004, Izumi et al., 2004, Lai and Orgogozo, 2004, Roegiers and Jan, 2004, Siegrist and Doe, 2004, Ahringer, 2003, Albertson and Doe, 2003, Cai et al., 2003, Fuse et al., 2003, Hidalgo and ffrench-Constant, 2003, Tuxworth and Chia, 2003, Wodarz and Huttner, 2003, Yu et al., 2003, Berdnik and Knoblich, 2002, Chia and Yang, 2002, Knust, 2001, Schaefer et al., 2001, Wodarz, 2001, Parmentier et al., 2000, Granderath et al., 1999, Greig and O'Kane, 1999.10.21, Mistry et al., 1995, Roberts et al., 1993, Roberts et al., 1993)
    Name Synonyms
    G protein I a-subunit 65A
    G protein alpha subunit
    G protein alpha-subunit
    G protein alphai subunit 65A
    G protein α i subunit
    G protein α o subunit 65A
    G protein αi subunit
    G protein αi subunit 65A
    G protein αo subunit 65A
    Secondary FlyBase IDs
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (203)