FB2020_06, released Dec 22, 2020

Gene: Dmel\DCTN1-p150

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General Information
Symbol
Dmel\DCTN1-p150
Species
D. melanogaster
Name
Dynactin 1, p150 subunit
Annotation Symbol
CG9206
Feature Type
protein_coding_gene
FlyBase ID
FBgn0001108
Gene Model Status
Current
Stock Availability
23 publicly available
Gene Snapshot
Dynactin 1, p150 subunit (DCTN1-p150) encodes the p150 subunit of the dynactin complex. Dynactin is thought to act as a dynein receptor that modulates binding of dynein to cellular cargoes and enhances the processivity of dynein movement. The roles of the product of DCTN1-p150 include oocyte polarity, mitotic cell division, embryonic development, neuronal transport and neurogenesis. [Date last reviewed: 2019-03-07]
Other Summaries
Also Known As

Glued, Gl, p150Glued, p150, dynactin

Key Links
Genomic Location
Cytogenetic map
70C5-70C6
Sequence location
3L:13,929,387..13,934,656 [+]
Recombination map

3-41

RefSeq locus
NT_037436 REGION:13929387..13934656
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (49 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
enables dynein complex binding
inferred from direct assay
(Morgan et al., 2011)
inferred from physical interaction with FLYBASE:Dhc64C; FB:FBgn0261797
(Siller et al., 2005)
enables dynein intermediate chain binding
inferred from physical interaction with FLYBASE:sw; FB:FBgn0003654
(Morgan et al., 2011)
enables microtubule binding
inferred from direct assay
(Lloyd et al., 2012)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables microtubule plus-end binding
inferred from biological aspect of ancestor with PANTHER:PTN000750989
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (30 terms)
Terms Based on Experimental Evidence (27 terms)
CV Term
Evidence
References
involved_in axon extension
inferred from genetic interaction with FLYBASE:Strip; FB:FBgn0035437
(Sakuma et al., 2014)
inferred from genetic interaction with FLYBASE:spri; FB:FBgn0085443
(Sakuma et al., 2014)
involved_in axonal transport of mitochondrion
inferred from mutant phenotype
(Pilling et al., 2006)
involved_in cell fate determination
inferred from mutant phenotype
(Fan and Ready, 1997)
involved_in cellularization
inferred from genetic interaction with FLYBASE:dop; FB:FBgn0267390
(Hain et al., 2014)
involved_in centrosome localization
inferred from mutant phenotype
(Jauffred et al., 2013)
involved_in centrosome separation
inferred from mutant phenotype
(Siller et al., 2005)
involved_in compound eye photoreceptor cell differentiation
inferred from mutant phenotype
(Fan and Ready, 1997)
involved_in compound eye photoreceptor development
inferred from mutant phenotype
(Fan and Ready, 1997)
involved_in establishment of epithelial cell apical/basal polarity
inferred from direct assay
(Li et al., 2008)
involved_in evoked neurotransmitter secretion
inferred from mutant phenotype
(Lloyd et al., 2012)
involved_in eye photoreceptor cell differentiation
inferred from mutant phenotype
(Whited et al., 2004)
involved_in intracellular protein transport
inferred from mutant phenotype
(Siller et al., 2005)
involved_in kinetochore organization
inferred from mutant phenotype
(Siller et al., 2005)
involved_in lumen formation, open tracheal system
inferred from mutant phenotype
(assigned by UniProt )
(Schottenfeld-Roames and Ghabrial, 2012)
involved_in melanotic encapsulation of foreign target
inferred from mutant phenotype
(Howell et al., 2012)
involved_in mitotic cell cycle
inferred from high throughput mutant phenotype
(Ducat et al., 2008)
involved_in mitotic spindle midzone assembly
inferred from mutant phenotype
(Delcros et al., 2006)
involved_in mRNA transport
inferred from mutant phenotype
(Delanoue and Davis, 2005)
involved_in positive regulation of axonogenesis
inferred from mutant phenotype
(Kudumala et al., 2017)
involved_in protein localization to kinetochore
inferred from mutant phenotype
(Dzhindzhev et al., 2005)
involved_in retrograde axonal transport
inferred from mutant phenotype
(Kudumala et al., 2017, Lloyd et al., 2012)
involved_in retrograde neuronal dense core vesicle transport
inferred from genetic interaction with FLYBASE:TBPH; FB:FBgn0025790
(Hosaka et al., 2017)
inferred from mutant phenotype
(Lloyd et al., 2012)
involved_in retrograde synaptic vesicle transport
inferred from direct assay
(Smith et al., 2012)
involved_in rhabdomere development
inferred from mutant phenotype
(Fan and Ready, 1997)
involved_in second mitotic wave involved in compound eye morphogenesis
inferred from mutant phenotype
(Fan and Ready, 1997)
involved_in spindle assembly
inferred from mutant phenotype
(Siller et al., 2005)
involved_in synaptic vesicle transport
inferred from genetic interaction with FLYBASE:Khc; FB:FBgn0001308
(Lloyd et al., 2012)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
involved_in cytoplasmic microtubule organization
inferred from biological aspect of ancestor with PANTHER:PTN000750989
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in establishment of mitotic spindle orientation
inferred from biological aspect of ancestor with PANTHER:PTN000431881
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in nuclear migration
inferred from biological aspect of ancestor with PANTHER:PTN000431881
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (15 terms)
Terms Based on Experimental Evidence (10 terms)
CV Term
Evidence
References
located_in aster
inferred from direct assay
(Delcros et al., 2006)
located_in axon cytoplasm
inferred from direct assay
(Pilling et al., 2006)
located_in centrosome
inferred from direct assay
(Delcros et al., 2006)
located_in cytoplasm
inferred from direct assay
(Lloyd et al., 2012)
colocalizes_with kinetochore microtubule
inferred from direct assay
(Siller et al., 2005)
colocalizes_with kinetochore
inferred from direct assay
(Siller et al., 2005)
located_in kinetochore
inferred from direct assay
(Delcros et al., 2006)
located_in midbody
inferred from direct assay
(Delcros et al., 2006)
located_in spindle microtubule
inferred from direct assay
(Delcros et al., 2006)
colocalizes_with spindle pole centrosome
inferred from direct assay
(Siller et al., 2005)
located_in type Ib terminal bouton
inferred from direct assay
(Lloyd et al., 2012)
Terms Based on Predictions or Assertions (7 terms)
CV Term
Evidence
References
located_in axon
inferred from biological aspect of ancestor with PANTHER:PTN000431881
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN000750989
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in kinetochore
inferred from biological aspect of ancestor with PANTHER:PTN000431881
(assigned by GO_Central )
(Gaudet et al., 2011)
part_of microtubule associated complex
inferred from biological aspect of ancestor with PANTHER:PTN000431881
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in microtubule plus-end
inferred from biological aspect of ancestor with PANTHER:PTN000750989
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000750989
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in spindle pole
inferred from biological aspect of ancestor with PANTHER:PTN000431881
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the dynactin 150 kDa subunit family. (P13496)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
DYNACTIN COMPLEX -
Dynactin is a large 1.2 MDa multisubunit protein complex that associates with the cytoplasmic dynein complex to drive microtubule-based transport. (Adapted from PMID:15473859 and PMID:25751425).
Protein Function (UniProtKB)
Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles.
(UniProt, P13496)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Gl: Glued
Eyes rough, smaller, and oblong; facets rounded; surface smooth and shiny like gl. Pattern, as indicated by staining with monoclonal antibodies, abnormal in the posterior eye disk (Renfrenz and Benzer, 1989, Dev. Biol. 136: 411-29). Architecture of the optic ganglia severely deranged; attributable to the mutant genotype of the overlying eye tissue (Meyerowitz and Kankel, 1978, Dev. Biol. 62: 112-42). Structures of individual neurons studied by S.H. Garen and Kankel (1983, Dev. Biol. 96: 445-56). Somatic-crossover studies and temperature-shift experiments with Glrv50, a temperature-sensitive allele, suggest that Gl+ gene product is required in mid third instar; abnormal retinula fiber projections first observed in late third instar [Harte and Kankel, 1983, Dev. Biol. 99: 88-102 (fig.)]. No electro-retinogram [Grossfield, 1975, Handbook of Genetics (R.C. King, ed.). Plenum Press, New York and London, Vol. 3, p. 690]. Bristles generally shortened slightly and straighter than normal. Viability and fertility of heterozygote good. With respect to severity of expression Df(3L)Gl2/+ = wild type; Gl/+/+/+ = very weak Glued; Gl/+/+ = weak Glued; Gl/+ = Glued; Gl/Gl/+ = few extreme Glued survivors, Gl/Df(3L)Gl-=Gl/Gl = lethal (Harte and Kankel). RK1.
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\DCTN1-p150 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene
gene_with_edited_transcript
(Graveley et al., 2011)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
DCTN1-p150-RA
FBtr0075756
NM_079337
4550
1265
Additional Transcript Data and Comments
Reported size (kB)

4.6 (compiled cDNA)

(Swaroop et al., 1987)

5.5 (northern blot)

(Swaroop et al., 1986)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
DCTN1-p150-PA
FBpp0075498
141.2
1265
5.32
NP_524061
AAF49788
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

135/150 (kD observed)

(Waterman-Storer and Holzbaur, 1996)

145/160 (kD observed)

(McGrail et al., 1995)

1319 (aa); 148 (kD predicted)

(Swaroop et al., 1987)
Comments
External Data
Subunit Structure (UniProtKB)

Large macromolecular complex of at least 10 components; p150(glued) binds directly to microtubules and to cytoplasmic dynein.

(UniProt, P13496)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\DCTN1-p150 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage & pupal stage
(Swaroop et al., 1986)
embryonic stage
(McGrail et al., 1995)
unfertilized egg stage
unfertilized egg
(Swaroop et al., 1986)
embryonic stage & larval stage & pupal stage
(Swaroop et al., 1986)
adult stage & oogenesis
ovary
(McGrail et al., 1995)
adult stage
adult head
(McGrail et al., 1995)
spermatogenesis & adult stage
testis
(McGrail et al., 1995)
third instar larval stage
(Swaroop et al., 1986)
Additional Descriptive Data

Gl transcript is detected throughout embryonic development. It is also detected in the head, ovary, and testis of adult flies.

(McGrail et al., 1995)

Gl transcript is expressed at high levels in embryos, but expression decreases in first and second instar larvae. Expression levels increase once more in third instar larvae and pupae. Gl is also expressed in unfertilized eggs. By in situ hybridization, Gl is detected in all embryonic, late larval and pupal tissues.

(Swaroop et al., 1986)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage & oogenesis stage S9
oocyte | posterior
(McGrail et al., 1995)
adult stage & oogenesis
germarium region 2b & oocyte
(McGrail et al., 1995)
oogenesis stage S1
germarium | anterior
(McGrail et al., 1995)
adult stage | femaleoogenesis stage S8 -- S10
oocyte | posterior
(Sanghavi et al., 2012)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood & male -- day 7 of adulthood & male
spermatozoon
(Dorus et al., 2006)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
adult stage
adult head
• membrane
(Aradska et al., 2015)
Additional Descriptive Data

During early oogenesis, Gl protein is distributed throughout all cells in the anterior germarium. It subsequently accumulates in the 1 cell destined to be the oocyte in germarium region 2b. At stage S9, it localizes to the posterior pole of the oocyte. Gl protein distribution is indistinguishable from that of cytoplasmic dynein. When immunolocalization is performed in a background mutant for cytoplasmic dynein, Gl protein mislocalizes with cytoplasmic dynein.

(McGrail et al., 1995)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in aster
inferred from direct assay
(Delcros et al., 2006)
located_in axon cytoplasm
inferred from direct assay
(Pilling et al., 2006)
located_in centrosome
inferred from direct assay
(Delcros et al., 2006)
located_in cytoplasm
inferred from direct assay
(Lloyd et al., 2012)
colocalizes_with kinetochore microtubule
inferred from direct assay
(Siller et al., 2005)
colocalizes_with kinetochore
inferred from direct assay
(Siller et al., 2005)
located_in kinetochore
inferred from direct assay
(Delcros et al., 2006)
located_in midbody
inferred from direct assay
(Delcros et al., 2006)
located_in spindle microtubule
inferred from direct assay
(Delcros et al., 2006)
colocalizes_with spindle pole centrosome
inferred from direct assay
(Siller et al., 2005)
located_in type Ib terminal bouton
inferred from direct assay
(Lloyd et al., 2012)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\DCTN1-p150 in GBrowse 2
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 59 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 19 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of DCTN1-p150
Transgenic constructs containing regulatory region of DCTN1-p150
Deletions and Duplications ( 3 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal | recessive
lethal | recessive | heat sensitive
lethal - all die before end of embryonic stage | recessive
lethal - all die before end of first instar larval stage (with DCTN1-p150l1)
lethal - all die before end of first instar larval stage (with DCTN1-p150Δ22)
lethal - all die before end of larval stage, with Scer\GAL4c311
lethal - all die before end of larval stage, with Scer\GAL4elav-C155
lethal - all die before end of pupal stage, with Scer\GAL4elav-C155
lethal - all die before end of pupal stage, with Scer\GAL4pnr-MD237
lethal - all die before end of pupal stage (with DCTN1-p150G38S)
lethal - all die before end of pupal stage (with DCTN1-p150l1)
lethal - all die before end of pupal stage (with DCTN1-p150Δ22)
lethal - all die before end of pupal stage | recessive
lethal - all die before end of pupal stage | recessive | heat sensitive
lethal - all die before end of second instar larval stage (with Df(3L)fz-GF3b)
lethal - all die before end of second instar larval stage | recessive
partially lethal, with Scer\GAL4dimm-929
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4tin.CΔ4
viable | heat sensitive
Sterility
Allele
female sterile | dominant
fertile
male fertile
sterile
Other Phenotypes
Allele
cell polarity defective
cell shape defective | adult stage | somatic clone
decreased cell number
decreased cell number | adult stage
eye color defective | dominant
flightless
immune response defective | larval stage, with Scer\GAL4Hml.PG
increased cell death | third instar larval stage
increased occurrence of cell division | third instar larval stage
locomotor behavior defective | adult stage | progressive, with Scer\GAL4ple.PF
locomotor behavior defective | adult stage | recessive
mitotic cell cycle defective
mitotic cell cycle defective (with Df(3L)fz-GF3b)
neuroanatomy defective
neuroanatomy defective, with Scer\GAL4c17
neuroanatomy defective, with Scer\GAL4elav-C155
neuroanatomy defective, with Scer\GAL4RapGAP1-OK6
neuroanatomy defective, with Scer\GAL4αTub84B.PP
neuroanatomy defective | adult stage
neuroanatomy defective | adult stage, with Scer\GAL4shot-OK307
neuroanatomy defective | adult stage | dominant
neuroanatomy defective | conditional, with Scer\GAL4unspecified
neuroanatomy defective | larval stage
neuroanatomy defective | progressive, with Scer\GAL4E49
neuroanatomy defective | pupal stage
neuroanatomy defective | third instar larval stage, with Scer\GAL4Toll-6-D42
neuroanatomy defective | third instar larval stage (with DCTN1-p150G38S)
neuroanatomy defective | third instar larval stage (with DCTN1-p150Δ22)
neurophysiology defective
neurophysiology defective, with Scer\GAL4c17
neurophysiology defective, with Scer\GAL4E49
neurophysiology defective, with Scer\GAL4elav-C155
neurophysiology defective | adult stage
neurophysiology defective | adult stage | dominant
neurophysiology defective | third instar larval stage
neurophysiology defective | third instar larval stage, with Scer\GAL4RapGAP1-OK6
neurophysiology defective | third instar larval stage (with DCTN1-p150G38S)
neurophysiology defective | third instar larval stage (with DCTN1-p150Δ22)
paralytic | larval stage | heat sensitive
proboscis extension reflex defective | progressive, with Scer\GAL4E49
short lived
short lived, with Scer\GAL4ple.PF
short lived, with Scer\GAL4RapGAP1-OK6
short lived, with Scer\GAL4Toll-6-D42
short lived | recessive
size defective | adult stage
size defective | adult stage, with Scer\GAL4shot-OK307
size defective | third instar larval stage, with Scer\GAL4RapGAP1-OK6
size defective | third instar larval stage, with Scer\GAL4Toll-6-D42
some die during embryonic stage | recessive
some die during first instar larval stage (with DCTN1-p150l1)
some die during first instar larval stage (with DCTN1-p150Δ22)
some die during larval stage, with Scer\GAL4c311
some die during larval stage, with Scer\GAL4elav-C155
some die during pupal stage, with Scer\GAL4elav-C155
some die during pupal stage, with Scer\GAL4pnr-MD237
some die during pupal stage (with DCTN1-p150G38S)
some die during pupal stage (with DCTN1-p150l1)
some die during pupal stage (with DCTN1-p150Δ22)
some die during pupal stage | recessive
some die during pupal stage | recessive | heat sensitive
some die during second instar larval stage (with Df(3L)fz-GF3b)
some die during second instar larval stage | recessive
uncoordinated, with Scer\GAL4dimm-929
visible
visible, with Scer\GAL4Act5C.PU
visible, with Scer\GAL4CCAP.PP
visible, with Scer\GAL4elav-C155
visible, with Scer\GAL4GMR.PF
visible, with Scer\GAL4hs.2sev
visible | adult stage
visible | dominant
visible | recessive
wild-type
Phenotype manifest in
Allele
adult CSD interneuron, with Scer\GAL4αTub84B.PP
adult neuromuscular junction, with Scer\GAL4E49
adult optic lobe
axon
axon, with Scer\GAL4Toll-6-D42
border follicle cell, with Scer\GAL4upd1.PU
bouton, with Scer\GAL4elav-C155
centrosome, with Scer\GAL4neur-P72
cone cell
cuticle
cyst progenitor cell, with Scer\GAL4C587
embryonic/larval dorsal branch | embryonic stage, with Scer\GAL4btl.PU
embryonic/larval neuron, with Scer\GAL4ap-md544
external sensory organ precursor cell I, with Scer\GAL4neur-P72
eye
eye, with Scer\GAL4Act5C.PU
eye, with Scer\GAL4elav-C155
eye, with Scer\GAL4GMR.PF
eye, with Scer\GAL4hs.2sev
eye disc, with Scer\GAL4c311
eye disc | third instar larval stage
eye photoreceptor cell
eye photoreceptor cell & microtubule
eye photoreceptor cell & nucleus
eye photoreceptor cell & nucleus, with Scer\GAL4Glass38-1
follicle cell
follicle cell | somatic clone
ganglion mother cell | larval stage
giant fiber neuron
giant fiber neuron, with Scer\GAL4c17
giant fiber neuron | adult stage, with Scer\GAL4GMR68A06
giant fiber neuron | adult stage, with Scer\GAL4shot-OK307
giant fibers & nerve terminal
giant fibers & nerve terminal, with Scer\GAL4c17
giant fibers & synapse
giant fibers & synapse, with Scer\GAL4c17
Golgi apparatus
interommatidial bristle
interommatidial bristle, with Scer\GAL4GMR.PF
larval Bolwig's organ & nucleus, with Scer\GAL4elav.PLu
lipid droplet | maternal effect
macrochaeta
mesothoracic femoral chordotonal organ & axon
metathoracic femoral chordotonal organ & axon
mitochondrion
mitochondrion, with Scer\GAL4Toll-6-D42
morphogenetic furrow, with Scer\GAL4c311
motor neuron
nervous system & thorax & pupa
neuronal dense core vesicle | third instar larval stage, with Scer\GAL4Toll-6-D42
NMJ bouton, with Scer\GAL4VGlut-OK371
NMJ bouton | third instar larval stage (with DCTN1-p150G38S)
NMJ bouton | third instar larval stage (with DCTN1-p150Δ22)
ommatidium
ommatidium, with Scer\GAL4Act5C.PU
ommatidium, with Scer\GAL4GMR.PF
ommatidium | adult stage
photoreceptor cell
photoreceptor cell R1
photoreceptor cell R6
photoreceptor cell R7 | somatic clone, with Scer\GAL4Act5C.PP
pigment cell
posterior fascicle & axon
postsynaptic membrane, with Scer\GAL4elav-C155
presynaptic active zone | third instar larval stage, with Scer\GAL4RapGAP1-OK6
primary pigment cell
proboscis muscle, with Scer\GAL4E49
prothoracic femoral chordotonal organ & axon
rhabdomere
secondary pigment cell
segmental nerve | conditional, with Scer\GAL4unspecified
sensillum trichodeum & leg & axon
sensory neuron
spindle
spindle, with Scer\GAL4C587
spindle (with DCTN1-p150l1)
spindle (with DCTN1-p150Δ22)
spindle pole centrosome
spindle pole centrosome (with DCTN1-p150l1)
spindle pole centrosome (with DCTN1-p150Δ22)
synapse
synapse, with Scer\GAL4elav-C155
synapse, with Scer\GAL4RapGAP1-OK6
synapse & neuromuscular junction, with Scer\GAL4elav-C155
synaptic vesicle | third instar larval stage, with Scer\GAL4RapGAP1-OK6
terminal bouton | third instar larval stage, with Scer\GAL4RapGAP1-OK6
terminal bouton | third instar larval stage, with Scer\GAL4Toll-6-D42
tertiary pigment cell
transport vesicle | adult stage, with Scer\GAL4GMR68A06
type Ib terminal bouton | third instar larval stage, with Scer\GAL4Toll-6-D42
wing
wing, with Scer\GAL4CCAP.PP
wing, with Scer\GAL4dimm-929
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (9)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\DCTN1
13 of 15
Yes
Yes
2  
Hsap\CLIP1
2 of 15
No
No
2  
Hsap\CLIP2
2 of 15
No
No
2  
Hsap\CLIP3
2 of 15
No
No
Hsap\CEP350
1 of 15
No
Yes
Hsap\CLIP4
1 of 15
No
No
Hsap\MMP15
1 of 15
No
No
Hsap\SLC4A5
1 of 15
No
No
Hsap\TBCB
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (8)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Dctn1
14 of 15
Yes
Yes
Mmus\Clip1
2 of 15
No
No
Mmus\Clip2
2 of 15
No
No
Mmus\Clip3
2 of 15
No
No
Mmus\Ccdc187
1 of 15
No
Yes
Mmus\Cep350
1 of 15
No
Yes
Mmus\Clip4
1 of 15
No
No
Mmus\Tbcb
1 of 15
No
No
Rattus norvegicus (Norway rat) (7)
Rnor\Dctn1
11 of 13
Yes
Yes
Rnor\Ccdc187
1 of 13
No
Yes
Rnor\Cep350
1 of 13
No
Yes
Rnor\Clip2
1 of 13
No
No
Rnor\Clip3
1 of 13
No
No
Rnor\LOC100911911
1 of 13
No
Yes
Rnor\LOC103690005
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (4)
Xtro\dctn1
9 of 12
Yes
Yes
Xtro\clip3
2 of 12
No
Yes
Xtro\clip1
1 of 12
No
No
Xtro\tbcb
1 of 12
No
No
Danio rerio (Zebrafish) (8)
Drer\dctn1b
10 of 15
Yes
Yes
Drer\dctn1a
9 of 15
No
Yes
Drer\clip1a
2 of 15
No
No
Drer\clip2
2 of 15
No
No
Drer\clip3
2 of 15
No
No
Drer\cep350
1 of 15
No
Yes
Drer\clip1b
1 of 15
No
No
Drer\tbcb
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (4)
Cele\dnc-1
13 of 15
Yes
Yes
Cele\clip-1
3 of 15
No
No
Cele\F59A6.5
1 of 15
No
No
Cele\tbcb-1
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (1)
Atha\EMB2804
2 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (3)
Scer\BIK1
3 of 15
Yes
No
Scer\NIP100
3 of 15
Yes
No
Scer\ALF1
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (3)
Spom\ssm4
3 of 12
Yes
Yes
Spom\tip1
2 of 12
No
No
Spom\alp11
1 of 12
No
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091901HP )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00004018
Drosophila simulans
Dsim\GD14462
Drosophila sechellia
Dsec\GM25436
Drosophila erecta
Dere\GG15657
Drosophila yakuba
Dyak\GE21986
Drosophila ananassae
Dana\GF24048
Drosophila pseudoobscura pseudoobscura
Dpse\GA21612
Drosophila persimilis
Dper\GL15715
Drosophila willistoni
Dwil\GK10424
Drosophila virilis
Dvir\GJ14081
Drosophila mojavensis
Dmoj\GI13734
Drosophila grimshawi
Dgri\GH15086
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500S8 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17012851
Glossina morsitans
Tsetse fly
Gmor\GMOY000345
Mayetiola destructor
Hessian fly
Mdes\Mdes006368
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL006145
Anopheles darlingi
American malaria mosquito
Adar\ADAC008045
Anopheles gambiae
Malaria mosquito
Agam\AGAP004194
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ003549
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ001544
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02U0 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA003806
Danaus plexippus
Monarch butterfly
Dple\DPOGS207366-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS211500-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL013227
Apis florea
Little honeybee
Aflo\2379349348
Apis mellifera
Western honey bee
Amel\GB50038
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP14997
Bombus terrestris
Buff-tailed bumblebee
Bter\15205614
Linepithema humile
Argentine ant
Lhum\LH18252
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:002cdd
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG002051
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_07565
Tribolium castaneum
Red flour beetle
Tcas\TC012455
Pediculus humanus
Human body louse
Phum\PHUM050910
Rhodnius prolixus
Kissing bug
Rpro\RPRC010724
Cimex lectularius
Bed bug
Clec\CLEC011935
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI21232
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI007344
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_14168
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00NL )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR007209
Ixodes scapularis
Black-legged tick
Isca\ISCW010118
Stegodyphus mimosarum
African social velvet spider
Smim\KFM67674.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM67675.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur30g01460
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_257531
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_312250
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0WO0 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_010290gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Dynact1ph
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000005319
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000026129
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (3)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
CG9279
7 of 10
CLIP-190
4 of 10
TBCB
2 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 3 )
Potential Models Based on Orthology ( 3 )
Modifiers Based on Experimental Evidence ( 3 )
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
DCTN1; dynactin subunit 1
13 of 15
601143
 
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser
View in Interactions Browser
Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
DCTN1-p150 - Arfip
anti tag coimmunoprecipitation, anti tag western blot, cosedimentation, western blot
(Chang et al., 2013)
DCTN1-p150 - Arp1
anti tag coimmunoprecipitation, anti tag western blot, experimental knowledge based
(Guruharsha et al., 2011, Kim et al., 2007)
DCTN1-p150 - Arp10
experimental knowledge based
(Guruharsha et al., 2011)
DCTN1-p150 - DCTN2-p50
anti tag coimmunoprecipitation, western blot, experimental knowledge based, anti tag western blot
(Guruharsha et al., 2011, Kim et al., 2007, Basto et al., 2004)
DCTN1-p150 - DCTN3-p24
experimental knowledge based
(Guruharsha et al., 2011)
DCTN1-p150 - DCTN4-p62
experimental knowledge based
(Guruharsha et al., 2011)
DCTN1-p150 - Dhc64C
anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot, cosedimentation through density gradient
(Kim et al., 2007, Papoulas et al., 2005, Siller et al., 2005)
DCTN1-p150 - Kap3
pull down, western blot
(Doodhi et al., 2009)
DCTN1-p150 - LRR
experimental knowledge based
(Guruharsha et al., 2011)
DCTN1-p150 - Lis-1
anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation
(Siller et al., 2005)
DCTN1-p150 - Nnf1a
anti tag coimmunoprecipitation, peptide massfingerprinting
(Przewloka et al., 2007)
DCTN1-p150 - Strip
two hybrid, anti bait coimmunoprecipitation, western blot, pull down, anti tag western blot
(Sakuma et al., 2014)
DCTN1-p150 - aurA
anti bait coimmunoprecipitation, anti tag western blot, enzymatic study, autoradiography, anti tag coimmunoprecipitation, western blot
(Romé et al., 2010)
DCTN1-p150 - egl
anti tag coimmunoprecipitation, western blot
(McClintock et al., 2018)
DCTN1-p150 - lva
pull down, western blot, anti tag western blot, anti bait coimmunoprecipitation
(Lin et al., 2015, Papoulas et al., 2005)
DCTN1-p150 - nuf
pull down, western blot
(Calero-Cuenca et al., 2016)
DCTN1-p150 - rod
anti tag coimmunoprecipitation, western blot
(Basto et al., 2004)
DCTN1-p150 - sw
pull down, western blot, nuclear magnetic resonance, comigration in non denaturing gel electrophoresis, molecular weight estimation by staining, anti bait coimmunoprecipitation
(Dix et al., 2013, Sanghavi et al., 2013, Nyarko et al., 2012, Reis et al., 2012, Morgan et al., 2011)
DCTN1-p150 - αTub67C
cosedimentation through density gradient, molecular weight estimation by staining
(Lloyd et al., 2012)
DCTN1-p150 - αTub84B
cosedimentation through density gradient, molecular weight estimation by staining
(Lloyd et al., 2012)
DCTN1-p150 - βTub56D
cosedimentation through density gradient, molecular weight estimation by staining
(Lloyd et al., 2012)
DCTN1-p150 - βTub60D
cosedimentation through density gradient, molecular weight estimation by staining
(Lloyd et al., 2012)
DCTN1-p150 - γTub23C
cosedimentation through density gradient, molecular weight estimation by staining
(Lloyd et al., 2012)
RNA-protein
Physical Interaction
Assay
References
DCTN1-p150 - bcd
anti bait coimmunoprecipitation, primer specific pcr
(Sanghavi et al., 2013)
DCTN1-p150 - fs(1)K10
pull down, western blot, peptide massfingerprinting
(Dix et al., 2013)
DCTN1-p150 - h
pull down, peptide massfingerprinting
(Dix et al., 2013)
DCTN1-p150 - osk
anti bait coimmunoprecipitation, primer specific pcr
(Sanghavi et al., 2013)
DCTN1-p150 - polo
pull down, Identification by mass spectrometry
(Oliveira et al., 2019)
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement
View in Interactions Browser
Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
DCTN1-p150
suppressible
AkhR
(Chang et al., 2013)
DCTN1-p150
suppressible
aPKC
(Ma et al., 2009)
DCTN1-p150
enhanceable
Arfip
(Chang et al., 2013)
DCTN1-p150
enhanceable
B4
(Chang et al., 2013)
DCTN1-p150
enhanceable
bbg
(Chang et al., 2013)
DCTN1-p150
enhanceable
CG4562
(Chang et al., 2013)
DCTN1-p150
enhanceable
Coop
(Chang et al., 2013)
DCTN1-p150|S
enhanceable
Dhc64C
(Iyadurai et al., 2008)
DCTN1-p150
suppressible
Dhc64C
(Iyadurai et al., 2008, McGrail et al., 1995, Harte and Kankel, 1982)
DCTN1-p150
enhanceable
Dhc64C
(McGrail et al., 1995)
DCTN1-p150
enhanceable
dop
(Hain et al., 2014)
DCTN1-p150
enhanceable
EG7
(Ma et al., 2009)
DCTN1-p150
enhanceable
EG28
(Ma et al., 2009)
DCTN1-p150
enhanceable
EG56
(Ma et al., 2009)
DCTN1-p150
enhanceable
EG79
(Ma et al., 2009)
DCTN1-p150
enhanceable
EG149
(Ma et al., 2009)
DCTN1-p150
enhanceable
EG162
(Ma et al., 2009)
DCTN1-p150
enhanceable
EG165
(Ma et al., 2009)
DCTN1-p150
enhanceable
fan
(Chang et al., 2013)
DCTN1-p150
suppressible
Khc
(Lloyd et al., 2012, Whited et al., 2004)
DCTN1-p150
enhanceable
Khc
(Lloyd et al., 2012)
DCTN1-p150
enhanceable
klar
(Chang et al., 2013)
DCTN1-p150
enhanceable
Lis-1
(Lei and Warrior, 2000)
DCTN1-p150
enhanceable
NaPi-III
(Chang et al., 2013)
DCTN1-p150
enhanceable
nesd
(Chang et al., 2013)
DCTN1-p150
enhanceable
S
(Iyadurai et al., 2008)
DCTN1-p150
enhanceable
sev
(Ting et al., 2007)
DCTN1-p150
suppressible
SG13
(Ma et al., 2009)
DCTN1-p150
suppressible
SG16
(Ma et al., 2009)
DCTN1-p150
suppressible
SG46
(Ma et al., 2009)
DCTN1-p150
enhanceable
Sirt2
(Chang et al., 2013)
DCTN1-p150
suppressible
spi
(Iyadurai et al., 2008)
DCTN1-p150
suppressible
Su(Gl)27
(Harte and Kankel, 1982)
DCTN1-p150
suppressible
Su(Gl)57
(Harte and Kankel, 1982)
DCTN1-p150
suppressible
Su(Gl)160
(Harte and Kankel, 1982)
DCTN1-p150
enhanceable
Su(H)
(Ma et al., 2009)
DCTN1-p150
enhanceable
sw
(Whited et al., 2004, Boylan and Hays, 2002)
DCTN1-p150
suppressible
sw
(Boylan et al., 2000)
DCTN1-p150
suppressible
TBPH
(Hosaka et al., 2017)
DCTN1-p150
enhanceable
TBPH
(Hosaka et al., 2017)
DCTN1-p150
enhanceable
TM9SF4
(Chang et al., 2013)
DCTN1-p150
enhanceable
β-Spec
(Lorenzo et al., 2010)
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
ctp
enhanceable
DCTN1-p150
(Ghosh-Roy et al., 2005, Ghosh-Roy et al., 2004)
CycB
suppressible
DCTN1-p150
(Ji et al., 2002)
DCTN2-p50
enhanceable
DCTN1-p150
(Duncan and Warrior, 2002)
Dl
suppressible
DCTN1-p150
(Iyadurai et al., 2008)
dop
enhanceable
DCTN1-p150
(Hain et al., 2014)
Egfr
enhanceable
DCTN1-p150
(Iyadurai et al., 2008)
Egfr
suppressible
DCTN1-p150
(Iyadurai et al., 2008)
Khc
enhanceable
DCTN1-p150
(Martin et al., 1999)
Lis-1
enhanceable
DCTN1-p150
(Lei and Warrior, 2000)
rho
suppressible
DCTN1-p150
(Iyadurai et al., 2008)
Strip
enhanceable
DCTN1-p150
(Sakuma et al., 2014)
TBPH
suppressible
DCTN1-p150
(Hosaka et al., 2017)
TBPH
enhanceable
DCTN1-p150
(Hosaka et al., 2017)
β-Spec
enhanceable
DCTN1-p150
(Lorenzo et al., 2010)
External Data
Subunit Structure (UniProtKB)
Large macromolecular complex of at least 10 components; p150(glued) binds directly to microtubules and to cytoplasmic dynein.
(UniProt, P13496 )
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
External Data
Linkouts
Genomic Location and Detailed Mapping Data
Chromosome (arm)
3L
Recombination map

3-41

Cytogenetic map
70C5-70C6
Sequence location
3L:13,929,387..13,934,656 [+]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
70C5-70C6
Limits computationally determined from genome sequence between P{lacW}l(3)L5212L5212&P{PZ}l(3)0587105871 and P{EP}EP3561EP3561
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
70C2-70C2
(determined by in situ hybridisation)
(Swaroop et al., 1986, Garen et al., 1984)
Experimentally Determined Recombination Data
Location

3-41

(FlyBase, 2016)

3-35.4

(Comeron et al., 2012)

3-41.4

Left of (cM)
(Trippa et al., 1971)
Right of (cM)
(Mossige, 1938, Coyne, 1935)
Notes
Stocks and Reagents
Stocks (23)
Genomic Clones (16)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (25)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
 

polyclonal

(Chang et al., 2013, Waterman-Storer and Holzbaur, 1996)
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of

Source for identity of: Gl CG9206

(Bayraktaroglu, 1999.11)

Source for identity of: DCTN1-p150 Gl

(Hays et al., 2015.6.30)
Source for database merge of
Additional comments
Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes spindle pole detachment when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

(Kiger et al., 2003)

The dynactin complex, (which contains Gl and Arp87C) functions locally within the presynaptic arbor to promote synapse stability.

(Eaton et al., 2002)

The Gl product has a role in assembling both the chemical and electrical components of the giant fiber synapse, suggesting that retrograde signalling may be essential for synapse formation and maturation.

(Allen et al., 1999)

Mutations in Gl disrupt fast organelle transport in both directions in axons.

(Martin et al., 1999)

After reaching the CNS, mutant axons exhibit errors in branching within the target domain.

(Murphey et al., 1999)

Gl, a component of the dynein-dynactin complex, appears to have a role in normal terminal branching, synaptogenesis and stabilisation of some sensory neurons.

(Murphey et al., 1999)

Mutation of Gl causes defects in mitosis, nuclear migration, cell fate determination, rhabdomere morphogenesis and cell death.

(Fan and Ready, 1997)

Mutants alter the anatomy of axons of the femoral chordotonal organ and disrupt the resistance reflex between the sensory neurons of the chordotonal organ and the tibial extensor motor neurons. Disruption of the dynein-dynactin complex disrupts sensory axon path finding during metamorphosis, and this in turn disrupts synaptic connectivity.

(Reddy et al., 1997)

17 EMS-induced lethal mutants are lethal in combination with either Gl1 or Df(3L)Gl2 and appear to be point mutations, since they complement lethals in flanking loci. The time of death varies. Approximately one-fourth (22/96) revertants of Gl induced by X or γ irradiation fail to complement one or more flanking lethals and are therefore presumed to be deficiencies; those induced by EMS and hybrid dysgenesis on the other hand complement all flanking lethals. Phenotypic revertants induced by ionizing radiation and EMS remain lethal in heterozygous combination with either Gl or Df(3L)Gl2 and with each other; revertants tested in combination with Df(3L)Gl2 die during the first larval instar. The majority of hybrid-dysgenesis-induced revertants (67/78) are viable in heterozygous combination with Df(3L)Gl2 and most "Gll" alleles, whereas they are lethal in combination with Gl1, Gll9, and Gll10; 7/67 are viable in all the above combinations and 4/67 are inviable in all combinations.

(Lindsley and Zimm, 1992)

Mutant alleles showed no interaction with mw2.

(Rabinow and Birchler, 1990)

Adult eye is rough with irregular facets, rhabdomere number per ommatidia is irregular and eye disc cluster pattern is irregular.

(Renfranz and Benzer, 1989)

Somatic-crossover studies and temperature-shift experiments with Glrv50, a temperature-sensitive allele, suggest that Gl+ gene product is required in mid-third instar; abnormal retinula fiber projections first observed in late third instar. Bristles generally shortened slightly and straighter than normal. Viability and fertility of heterozygote good.

(Harte and Kankel, 1983)

Architecture of the optic ganglia severely deranged; attributable to the mutant genotype of the overlying eye tissue.

(Meyerowitz and Kankel, 1978)
Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 35 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
Other crossreferences
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
InterPro - A database of protein families, domains and functional sites
KEGG Genes - Molecular building blocks of life in the genomic space.
modMine - A data warehouse for the modENCODE project
Linkouts
ApoDroso - Functional genomic database for photoreceptor development, survival and function
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
FlyMine - An integrated database for Drosophila genomics
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Synonyms and Secondary IDs (28)
Reported As
Symbol Synonym
CG9206
(Meadows, 2017.1.11, Çiçek et al., 2016, Butzlaff et al., 2015, Hosono et al., 2015, Howell et al., 2012, Cammarato et al., 2011, Perkins et al., 2009, Ducat et al., 2008, Goshima et al., 2007, Przewloka et al., 2007, Eckley and Schroer, 2003, Goldstein and Gunawardena, 2000, Goldstein and Gunawardena, 2000)
DCTN-P
(Yuan et al., 2019)
DCTN1
(Kreko-Pierce and Eaton, 2017, Kollmar, 2015.9.21, Hammesfahr and Kollmar, 2012)
DCTN1-p150
(Jiang et al., 2019, Yuan et al., 2019, Transgenic RNAi Project members, 2017-, Hays et al., 2015.6.30)
DYNA_DROME
(Storm and Sonnhammer, 2003)
Dctn 1-p150
(Oliveira et al., 2019)
FCP-D
(Horne-Badovinac et al., 2012)
Gl
(Caviglia et al., 2016, Sarov et al., 2016, Ermanoska et al., 2014, Hain et al., 2014, Sakuma et al., 2014, Clark et al., 2012, Horne-Badovinac et al., 2012, Lloyd et al., 2012, Rawson et al., 2012, Reis et al., 2012, Smith et al., 2012, Wong et al., 2012, Cheng et al., 2011, Neumüller et al., 2011, Kracklauer, 2010.2.24, Lorenzo et al., 2010, Anderson et al., 2009, Christensen et al., 2009.2.28, Ma et al., 2009, Wang et al., 2009, Boylan et al., 2008, Li et al., 2008, Siller and Doe, 2008, Yang et al., 2008, Mische et al., 2007, Ting et al., 2007, Vendra et al., 2007, Dorus et al., 2006, Dorus et al., 2006, Pilling et al., 2006, Pramatarova et al., 2006, Wehr et al., 2006, Delanoue and Davis, 2005, Mathew et al., 2005, Mathew et al., 2005, Siller et al., 2005)
Glu
(Mosley-Bishop et al., 1999)
Glued
(Butzlaff et al., 2015, Herrero et al., 2007)
P150
(Miguel-Aliaga et al., 2008)
P150Glued
(Doodhi et al., 2009, Pilling et al., 2006)
P150A
(Kollmar and Odronitz, 2006)
gl
(Bosveld et al., 2016)
p150
(McClintock et al., 2018, Hosaka et al., 2017, Butzlaff et al., 2015, Lloyd et al., 2012, Reis et al., 2012, Lorenzo et al., 2010, Li et al., 2008, Dzhindzhev et al., 2005, Basto et al., 2004, Wojcik et al., 2001)
p150/Glued
(Welte, 2004)
p150/glued
(Keshishian and Kim, 2004, Tekotte and Davis, 2002)
p150Glued
(Nieuwburg et al., 2017, Lin et al., 2015, Dix et al., 2013, Nyarko et al., 2012, Morgan et al., 2011, Benseñor et al., 2010, Lorenzo et al., 2010, Rogers et al., 2008, Griffis et al., 2007, Haghnia et al., 2007, Delcros et al., 2006, Papoulas et al., 2005, Gunawardena et al., 2003, Kaltschmidt and Brand, 2002, Deng and Lin, 2001, Sisson et al., 2000)
p150glued
(Kim et al., 2007)
t1
Name Synonyms
Dynactin
(Ball et al., 2010, Januschke et al., 2007, Vendra et al., 2007, Delcros et al., 2006)
Dynactin 1, p150 subunit
Dynactin P150
(Schottenfeld-Roames and Ghabrial, 2012)
Dynactin1 p150 A
(Kollmar, 2015.9.15)
Glued
(Kudumala et al., 2017, Calero-Cuenca et al., 2016, Beaven et al., 2015, Del Castillo et al., 2015, Liu et al., 2015, Hain et al., 2014, Chang et al., 2013, Dix et al., 2013, Johnson et al., 2013, Lu and Johnston, 2013, Fabian and Brill, 2012, Howell et al., 2012, Lloyd et al., 2012, Nagaraj and Adler, 2012, Rawson et al., 2012, Reis et al., 2012, Sanghavi et al., 2012, Schottenfeld-Roames and Ghabrial, 2012, Smith et al., 2012, Cammarato et al., 2011, Van De Bor et al., 2011, Veverytsa and Allan, 2011, Ball et al., 2010, Bohm et al., 2010, Li et al., 2010, Liu et al., 2010, Losada-Pérez et al., 2010, Xiong et al., 2010, Anderson et al., 2009, Eade and Allan, 2009, Gaspar and Szabad, 2009, Ma et al., 2009, Wainman et al., 2009, Ducat et al., 2008, Horne-Badovinac and Bilder, 2008, Iyadurai et al., 2008, Li et al., 2008, Miguel-Aliaga et al., 2008, Siller and Doe, 2008, Yang et al., 2008, Goold and Davis, 2007, Goshima et al., 2007, Herrero et al., 2007, Mische et al., 2007, Przewloka et al., 2007, Roy et al., 2007, Ting et al., 2007, Vendra et al., 2007, Delcros et al., 2006, Pramatarova et al., 2006, Ghosh-Roy et al., 2005, Siller et al., 2005, Waterman-Storer and Holzbaur, 1996, McGrail et al., 1995)
dynactin
(Calero-Cuenca et al., 2016, Reis et al., 2012, Romé et al., 2010, Miguel-Aliaga et al., 2008, Siller and Doe, 2008, Solecki et al., 2006, Papoulas et al., 2005, Siller et al., 2005, Gunawardena et al., 2003)
glued
(Jauffred et al., 2013, Rawson et al., 2012)
p150-Glued
(Basto et al., 2004)
p150glued
(Romé et al., 2010)
p150glued
(Gunawardena et al., 2003)
Secondary FlyBase IDs
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (281)