Gene model reviewed during 5.49
gene_with_stop_codon_read_through ; SO:0000697
Stop-codon suppression (UGA) postulated; based on UniProt P18173.
Gene model reviewed during 6.15
None of the polypeptides share 100% sequence identity.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Gld using the Feature Mapper tool.
Gld transcript levels increase upon treatment with an exogenous source of ecdysterone. This can be reversed in ecd1 larvae, no increase in Gld mRNA levels is seen. Ecdysterone-responsiveness competence is gained by Gld during the third instar. Premature exposure of wild type mid-third instar larvae to ecdysterone results in the rapid accumulation of Gld transcripts.
The level of Gld transcripts rises 3- to 4-fold in late third instar larvae then declines rapidly. Transcripts are abundant in pupal stages 6-13 and in adult males. The larval transcript is found only in the integument and the male transcript is localized predominantly in the ejaculatory ducts.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Gld in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Gld CG1152
Interactions among proximal promoter elements and a cluster of intronically located enhancers and silencers specify the complex regulation of Gld.
Ecol\lacZ reporter gene constructs have identified an SREC (Somatic Reproductive Organ Enhancer Complex) in Gld intron 1 (+639 to +3906 nucleotides). A 361 nucleotide region of the SREC is involved in ejaculatory duct/oviduct-specific expression in both the developing and mature reproductive tract.
Site directed mutagenesis and transgenic analysis demonstrates that the palindrome in the Gld promoter region is both necessary and sufficient for expression in the anterior spiracular gland whereas the palindromic sequence is sufficient for expression in the ejaculatory bulb.
Protein encoded is member of GMC oxidoreductase family of flavoproteins.
Enzyme transferred from males to females during copulation. Lethality of null alleles in the pupal stage observed; Gld null flies can be rescued by pre-eclosion excision of pupal operculum.
Interspecific P-element-mediated gene transformation is used to assess possible cis- and trans-genetic differences in the expression of Gld and Dpse\Gld. Interspecific variation in adult Gld expression pattern is studied.
Misregulation of sex-determination genes during development has been shown to effect the expression of Gld.