FlyBase Gene Report: Dmel\Glt

General Information

Symbol

Dmel\Glt

Species

D. melanogaster

Name

Glutactin

Annotation Symbol

CG9280

Feature Type

protein_coding_gene

FlyBase ID

FBgn0001114

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Summary

Glutactin (Glt) encodes a secreted protein that belongs to the family of cholinesterase/carboxylesterase-like proteins but lacks the catalytically active residues. It forms part of the basement membrane and regulates axonal outgrowth. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

29E3-29E3

Sequence location

2L:8,672,698..8,676,804 [+]

Recombination map

2-33

RefSeq locus

NT_033779 REGION:8672698..8676804

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

NOT enables carboxylic ester hydrolase activity

inferred from key residues

(Olson et al., 1990)

NOT enables peptidase activity

inferred from key residues with MEROPS:S09.UNW

(GO curators, 2012-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in defense response to other organism

inferred from mutant phenotype

(Arefin et al., 2014)

involved_in hemolymph coagulation

inferred from direct assay

(Schmid et al., 2019)

involved_in motor neuron axon guidance

inferred from mutant phenotype

(Inaki et al., 2007)

involved_in synaptic target inhibition

inferred from mutant phenotype

(Inaki et al., 2007)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in basement membrane

inferred from direct assay

(Fessler et al., 1994, Olson et al., 1990)

located_in extracellular space

inferred from direct assay

(Schmid et al., 2019)

located_in vesicle lumen

inferred from direct assay

(Schmid et al., 2019)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

S9 NON-PEPTIDASE HOMOLOGS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

S9 NON-PEPTIDASE HOMOLOGS -: S9 non-peptidase homologs are similar in amino acid sequence to S9 serine peptidases but lack one or more of the expected catalytic residues. (Adapted from FBrf0239469.)

Protein Function (UniProtKB)

Not known. Binds calcium ions.

(UniProt, P33438)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Glt: Glutactin

Encodes an acidic sulfated glycoprotein of basement membranes.

Summary (Interactive Fly)

transmembrane - novel serine esterase homolog - functions in glia to ensure a secure blood-brain barrier - located to tricellular junctions and is critical for the formation of tricellular and septate junctions and permeability barrier function

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Glt for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P33438)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Gene model reviewed during 5.48

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Glt-RA

FBtr0079727

NM_058156

3373

1026

Glt-RD

FBtr0307091

NM_001259017

3260

1026

Additional Transcript Data and Comments

Reported size (kB)

3.8 (northern blot)

(Olson et al., 1990)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Glt-PA

FBpp0079331

118.7

1026

4.42

FBpp0297934

118.7

1026

4.42

P33438

NP_001245946

AFH03620

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1026 aa isoforms: Glt-PA, Glt-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

1023 (aa); 155 (kD observed)

(Olson et al., 1990)

Comments

External Data

Post Translational Modification

Extensively O-glycosylated and also N-glycosylated.

About four tyrosines are sulfated.

(UniProt, P33438)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Glt using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Glt_1
Glt_2

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.01

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 16

embryonic/larval fat body

(Fisher et al., 2012)

embryonic/larval plasmatocyte

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

presumptive embryonic/larval central nervous system

(Olson et al., 1990)

apodeme

(Olson et al., 1990)

larval thoracic perisympathetic organ

(Olson et al., 1990)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis stage S9 -- S10

border follicle cell

(Borghese et al., 2006)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

Kc167 cell-line

Comment: in conditioned medium

(Kumagai et al., 2000)

Additional Descriptive Data

In situ hybridization shows that Glt is enriched in border follicle cells relative to follicle cells in stage S9 or S10 egg chambers.

(Borghese et al., 2006)

Glt protein is found in basement membranes, especially the invaginated envelope of the embryonic CNS, muscle apodemes and dorsal median cell processes.

(Olson et al., 1990)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in basement membrane

inferred from direct assay

(Fessler et al., 1994, Olson et al., 1990)

located_in extracellular space

inferred from direct assay

(Schmid et al., 2019)

located_in vesicle lumen

inferred from direct assay

(Schmid et al., 2019)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Glt in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Glt

View exonic expression by tissue for Dmel\Glt

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001114

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34193/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001114

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001114

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001114

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9280

Flygut - An atlas of the Drosophila adult midgut

FBgn0001114

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Glt

Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Glt

Transgenic constructs containing regulatory region of Glt

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2L)Exel7039

(Ryder, 2004.4.26)

Inferred from location ( 11 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Glt^GD5017

viable, with Scer\GAL4^Mef2.PR

Glt^GD5017

viable, with Scer\GAL4^pnr-MD237

Glt^GD5017

viable, with Scer\GAL4^tin.CΔ4

Glt^GD5017

Other Phenotypes

Allele

abnormal immune response

Glt^EY22126

abnormal immune response, with Scer\GAL4^He.PZ

Glt^GD5017

abnormal immune response, with Scer\GAL4^Hml.PG

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Glt^GD5017

short lived | conditional, with Scer\GAL4^He.PZ

Glt^GD5017

short lived | conditional, with Scer\GAL4^Hml.PG

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (20)

Hsap\CES1

3 of 14

Yes

Hsap\CES4A

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\NCEH1

1 of 14

Hsap\TG

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (25)

Rnor\Ces1a

3 of 14

Yes

Rnor\Ces1d

3 of 14

Yes

Rnor\Ces1f

3 of 14

Yes

Rnor\Ces4a

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (25)

Mmus\Ces1a

3 of 14

Yes

Mmus\Ces1b

3 of 14

Yes

Mmus\Ces1c

3 of 14

Yes

Mmus\Ces1d

3 of 14

Yes

Mmus\Ces1e

3 of 14

Yes

Mmus\Ces1f

3 of 14

Yes

Mmus\Ces1g

3 of 14

Yes

Mmus\Ces4a

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (18)

Xtro\aadac

1 of 13

Yes

Xtro\acaa1

1 of 13

Yes

Xtro\angptl3

1 of 13

Yes

Xtro\bche

1 of 13

Yes

Xtro\bod1l1

1 of 13

Yes

Xtro\ces3.1

1 of 13

Yes

Xtro\ces3.5

1 of 13

Yes

Xtro\ces5a

1 of 13

Yes

Xtro\dmd.3

1 of 13

Yes

Xtro\gdf10

1 of 13

Yes

Xtro\lipe

1 of 13

Yes

Xtro\nceh1

1 of 13

Yes

Xtro\nlgn1

1 of 13

Yes

Xtro\nlgn3

1 of 13

Yes

Xtro\nlgn4x

1 of 13

Yes

Xtro\palm2akap2

1 of 13

Yes

Xtro\ptgr2

1 of 13

Yes

Xtro\unc93a.2

1 of 13

Yes

Danio rerio (Zebrafish) (17)

Drer\cel.1

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:dkey-30c15.17

1 of 14

Drer\tg

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (37)

Cele\cest-27

3 of 14

Yes

Cele\cest-30

3 of 14

Yes

Cele\cest-35.1

3 of 14

Yes

Cele\cest-35.2

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (43)

Agam\AgaP_AGAP010917

4 of 12

Yes

Agam\AgaP_AGAP011365

4 of 12

Yes

Agam\AgaP_AGAP011366

4 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (20)

Atha\AT1G19190

1 of 13

Yes

Atha\AT1G47480

1 of 13

Yes

Atha\AT1G49640

1 of 13

Yes

Atha\AT1G49650

1 of 13

Yes

Atha\AT1G68620

1 of 13

Yes

Atha\AT2G03550

1 of 13

Yes

Atha\AT2G45600

1 of 13

Yes

Atha\AT2G45610

1 of 13

Yes

Atha\AT3G27320

1 of 13

Yes

Atha\AT5G06570

1 of 13

Yes

Atha\CXE5

1 of 13

Yes

Atha\CXE12

1 of 13

Yes

Atha\CXE13

1 of 13

Yes

Atha\CXE16

1 of 13

Yes

Atha\CXE17

1 of 13

Yes

Atha\CXE18

1 of 13

Yes

Atha\CXE20

1 of 13

Yes

Atha\GID1A

1 of 13

Yes

Atha\GID1B

1 of 13

Yes

Atha\GID1C

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPCC417.12

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Glt. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (30)

5 of 13

5 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

gas

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

Jhe

3 of 13

clt

2 of 13

Est-6

2 of 13

Gli

2 of 13

Ace

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Hsl

1 of 13

Nlg3

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Glt^EY22126

exacerbates nervous system disease

modeled by Atpα^DTS1

(Talsma et al., 2014)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CES1; carboxylesterase 1

3 of 14

114835

DRUG METABOLISM, ALTERED, CES1-RELATED

CES4A; carboxylesterase 4A

3 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Glt - Art4

experimental knowledge based

(Guruharsha et al., 2011)

Glt - CCT3

experimental knowledge based

(Guruharsha et al., 2011)

Glt - CCT8

experimental knowledge based

(Guruharsha et al., 2011)

Glt - CG1371

experimental knowledge based

(Guruharsha et al., 2011)

Glt - CG3008

experimental knowledge based

(Guruharsha et al., 2011)

Glt - CG33303

experimental knowledge based

(Guruharsha et al., 2011)

Glt - CG9467

experimental knowledge based

(Guruharsha et al., 2011)

Glt - CaBP1

experimental knowledge based

(Guruharsha et al., 2011)

Glt - Chc

experimental knowledge based

(Guruharsha et al., 2011)

Glt - Cnx99A

experimental knowledge based

(Guruharsha et al., 2011)

Glt - Col4a1

experimental knowledge based

(Guruharsha et al., 2011)

Glt - Cyp12d1-p

experimental knowledge based

(Guruharsha et al., 2011)

Glt - Fim

experimental knowledge based

(Guruharsha et al., 2011)

Glt - Gp210

experimental knowledge based

(Guruharsha et al., 2011)

Glt - HnRNP-K

experimental knowledge based

(Guruharsha et al., 2011)

Glt - Idh3a

experimental knowledge based

(Guruharsha et al., 2011)

Glt - LanB2

experimental knowledge based

(Guruharsha et al., 2011)

Glt - Mpcp2

experimental knowledge based

(Guruharsha et al., 2011)

Glt - Nacα

experimental knowledge based

(Guruharsha et al., 2011)

Glt - Nup358

experimental knowledge based

(Guruharsha et al., 2011)

Glt - OstΔ

experimental knowledge based

(Guruharsha et al., 2011)

Glt - Pef

experimental knowledge based

(Guruharsha et al., 2011)

Glt - Pi3K68D

experimental knowledge based

(Guruharsha et al., 2011)

Glt - Pih1D1

experimental knowledge based

(Guruharsha et al., 2011)

Glt - Pxn

experimental knowledge based

(Guruharsha et al., 2011)

Glt - Rdh1

experimental knowledge based

(Guruharsha et al., 2011)

Glt - RpLP0

experimental knowledge based

(Guruharsha et al., 2011)

Glt - RpS10b

experimental knowledge based

(Guruharsha et al., 2011)

Glt - ScsβG

experimental knowledge based

(Guruharsha et al., 2011)

Glt - SerRS

experimental knowledge based

(Guruharsha et al., 2011)

Glt - TER94

experimental knowledge based

(Guruharsha et al., 2011)

Glt - UQCR-C2

experimental knowledge based

(Guruharsha et al., 2011)

Glt - Vps4

experimental knowledge based

(Guruharsha et al., 2011)

Glt - boca

experimental knowledge based

(Guruharsha et al., 2011)

Glt - bor

experimental knowledge based

(Guruharsha et al., 2011)

Glt - cPges

experimental knowledge based

(Guruharsha et al., 2011)

Glt - mEFTu1

experimental knowledge based

(Guruharsha et al., 2011)

Glt - mod

experimental knowledge based

(Guruharsha et al., 2011)

Glt - r

experimental knowledge based

(Guruharsha et al., 2011)

Glt - scf

experimental knowledge based

(Guruharsha et al., 2011)

Glt - vkg

experimental knowledge based

(Guruharsha et al., 2011)

Glt - α-Spec

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60312

DroID - A comprehensive database of gene and protein interactions.

FBgn0001114

MIST (protein-protein) - An integrated Molecular Interaction Database

34193

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-33

Cytogenetic map

29E3-29E3

Sequence location

2L:8,672,698..8,676,804 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

29E3-29E3

Limits computationally determined from genome sequence between P{lacW}Sema-1a^k13702 and P{lacW}l(2)k04003^k04003

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

29D-29D

(determined by in situ hybridisation)

(Olson et al., 1990)

Experimentally Determined Recombination Data

Location

2-33

(FlyBase, 2016)

2-28.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

56309

y¹ w^*; Mi{MIC}Glt^MI11349

22539

y¹ w^67c23; P{EPgy2}Glt^EY22126

78757

y¹ sc^* v¹ sev²¹; P{TKO.GS02457}attP40

55929

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04217}attP40

NIG-Fly

9280R-1

P{NIG.9280R}1

HMJ23403

y¹ v¹; P{TRiP.HMJ23403}attP40/CyO

M2L-0053

y¹ w¹¹¹⁸; Glt^SK1 P{neoFRT}40A/CyO-Tb

M2L-0054

y¹ w¹¹¹⁸; Glt^SK3 P{neoFRT}40A/CyO-Tb

2LG-0043

y² VhaAC39-1^cho-1 v¹; P{U6-Glt.NIG.sgRNA}attP40/CyO

VDRC

v101918

P{KK110236}VIE-260B

v15428

w¹¹¹⁸; P{GD5017}v15428

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (82)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

SD01663

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0001114

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0001114

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Kumagai et al., 2000)

polyclonal

(Gullberg et al., 1994, Olson et al., 1990)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

A region of the Nrt extracellular domain is sufficient for cell adhesion, cholinesterase-like domain (from Glt) may functionally replace the Nrt extracellular domain for adhesion properties.

(Darboux et al., 1996)

EST SD17528 is chimeric; the 5' portion matches part of AE on the opposite strand to Glt, the 3' portion corresponds to part of RpS8.

(FlyBase, 1996-)

In vitro fusion of the homologous extracellular domain of Egfr and Glt to the Nrt cytoplasmic domain can mediate aggregation of cells incubated with a soluble crude fraction containg Nrt ligand activity. The binding site for the Nrt ligand is localised within the extracellular domain. A stretch of 25 amino acids forms an alpha-helix located close to the pseudocatalytic site and is crucial for the adhesive function.

(Darboux et al., 1995)

Glt encodes an acidic sulphated glycoprotein of basement membranes.

(Olson et al., 1990)

Encodes an acidic sulfated glycoprotein of basement membranes.

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CG9280

(National Institute of Genetics Fly Stocks, 2023-, National Institute of Genetics Fly Stocks, 2022-, Palu and Thummel, 2016, Jaspers et al., 2012, Cammarato et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Goldman and Arbeitman, 2007, Fakhouri et al., 2006)

CT26441

(Hynes and Zhao, 2000)

Glt

(Pignataro et al., 2024, Vesala et al., 2024, Hultmark and Andó, 2022, National Institute of Genetics Fly Stocks, 2022-, Moussalem et al., 2021, Cho et al., 2020, Parra and Johnston, 2020, Dziedziech et al., 2019, Schmid et al., 2019, Duneau et al., 2017, Transgenic RNAi Project members, 2017-, Arefin et al., 2014, Zhu and Zhang, 2013, Japanese National Institute of Genetics, 2012.5.21, Nose, 2012, Liu et al., 2011, Inaki et al., 2007, Liu et al., 2005)

Glutactin

(Dziedziech et al., 2020)

glt

(Albert et al., 2018)

Name Synonyms

Glutactin

(Ramond et al., 2020, Banerjee et al., 2019, Schmid et al., 2019, Arefin et al., 2014, Felix et al., 2012, Jaspers et al., 2012, Nose, 2012, Cammarato et al., 2011, Goldman and Arbeitman, 2007, Inaki et al., 2007, Borghese et al., 2006, Fakhouri et al., 2006)

glutactin

(Eleftherianos and Heryanto, 2021, Arefin et al., 2014, Kumagai et al., 2000, Gullberg et al., 1994, Olson, 1990.7.26)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 42 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34193

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P33438

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P33438

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9NCS8

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P33438

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001114

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34193/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001114

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001114

FlyMine - An integrated database for Drosophila genomics

FBgn0001114

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG9280

MARRVEL_MODEL - MARRVEL (model organism gene)

34193

Linkouts

BioGRID - A database of protein and genetic interactions.

60312

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0001114

DroID - A comprehensive database of gene and protein interactions.

FBgn0001114

DRSC - Results frm RNAi screens

FBgn0001114

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Glt_1
Glt_2

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001114

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0001114

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9280

Flygut - An atlas of the Drosophila adult midgut

FBgn0001114

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

34193

References (89)

The following external sites may use different assemblies or annotations than FlyBase.

-1.01

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