G-oα47A, G-oalpha47A, Go, Goα, bkh
G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
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Up to stage 13 of embryogenesis, G-oα47A protein is detected only at low levels. After germ band retraction, and through the rest of embryogenesis, G-oα47A protein is detected in the neuropil of the brain and ventral ganglion.
G-oα47A protein is more abundant in heads than bodies. No changes in protein levels were seen in para, sev, ora and norpA mutants. G-oα47A protein levels are decreased in gust mutants and increased in rut, dnc and tur mutants and the over-expression is sex-dependent in the rut mutant.
G-oα47A protein is detected in the neuropil of the brain and the thoracic ganglion, and at lower levels in cortical regions. Some staining was detected in the distal lamina and in the synaptic zone of the lamina. The proximal half of the ocellar nerve, and a bundle of axons in the antennal nerve also stained for G-oα47A protein.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Gαo in GBrowse 2
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
G-oα47A is required for the transduction of both the Wnt and planar polarity pathways.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Area matching Drosophila GO protein alpha subunit homolog class II gene, Acc. No. M29602.
G-oα47A function is required for the formation of the heart, visceral musculature and the nervous system.
Mutants show defects in the heart, visceral musculature and nervous system.
Mutation rate at microsatellite loci in 119 lines maintained for approximately 250 generations is estimated to be 6.3x10-6, at least one order of magnitude lower than the mutation rate in mammals.
G proteins belong to a family of membrane-associated guanine nucleotide-binding proteins that couple specific receptors for extracellular signals to specific intracellular effectors, thus regulating the activity of these effectors. When not interacting with the receptor, G proteins are usually in the form of a heterotrimer made up of α, β and γ subunits, with the α subunit bound to GDP. Upon activation by the receptor, the α subunit exchanges GDP for GTP, dissociates from the β-γ subunits and interacts with the effector. Afterwards GTP is hydrolyzed and the heterotrimer of α, β and γ subunits is formed again.
Identification: Isolated from a genomic library using a bovine αi1 cDNA probe.
The gene is named "brokenheart" based on the phenotype of mutant embryos.