G-oα47A, G-oalpha47A, Go, Goα, bkh
G protein alpha subunit - regulates mesenchymal-epithelial transition that takes place in cardial cells and precursor cells of the visceral musculature
Please see the JBrowse view of Dmel\Gαo for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
39-40 (kD)
G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Gαo using the Feature Mapper tool.
Comment: maternally deposited
Comment: reported as dorsal vessel specific anlage
Comment: reference states 0-2 hr AEL
Comment: all isoforms except Go-RA, Go-RC and Go-RI (G-oα47A-RA, G-oα47A-RC and G-oα47A-RI)
RT-PCR analysis shows that all isoforms of G-oα47A except G-oα47A-RA, G-oα47A-RC and G-oα47A-RI are expressed in the adult head, third segment of the antenna and maxillary palps. The latter 3 isoforms are only detected in the adult head and third segment of the antenna.
G-oα47A protein is detected in the neuropil of the brain and the thoracic ganglion, and at lower levels in cortical regions. Some staining was detected in the distal lamina and in the synaptic zone of the lamina. The proximal half of the ocellar nerve, and a bundle of axons in the antennal nerve also stained for G-oα47A protein.
GBrowse - Visual display of RNA-Seq signals
View Dmel\Gαo in GBrowse 22-62
2-61.1
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: G-oα47A CG2204
Source for identity of: Gαo G-oα47A
FlyBase curator comment: Genes encoding Gα subunits that have been named for their molecular function/orthology have had their symbols and names standardized following the format used in FBrf0220456, which also reflects popular usage in the Drosophila literature.
G-oα47A is required for the transduction of both the Wnt and planar polarity pathways.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Area matching Drosophila GO protein alpha subunit homolog class II gene, Acc. No. M29602.
G-oα47A function is required for the formation of the heart, visceral musculature and the nervous system.
Mutants show defects in the heart, visceral musculature and nervous system.
Mutation rate at microsatellite loci in 119 lines maintained for approximately 250 generations is estimated to be 6.3x10-6, at least one order of magnitude lower than the mutation rate in mammals.
Mutant analysis demonstrates G-oα47A is required for embryonic development.
G proteins belong to a family of membrane-associated guanine nucleotide-binding proteins that couple specific receptors for extracellular signals to specific intracellular effectors, thus regulating the activity of these effectors. When not interacting with the receptor, G proteins are usually in the form of a heterotrimer made up of α, β and γ subunits, with the α subunit bound to GDP. Upon activation by the receptor, the α subunit exchanges GDP for GTP, dissociates from the β-γ subunits and interacts with the effector. Afterwards GTP is hydrolyzed and the heterotrimer of α, β and γ subunits is formed again.
Identification: Isolated from a genomic library using a bovine αi1 cDNA probe.
The gene is named "brokenheart" based on the phenotype of mutant embryos.