FlyBase Gene Report: Dmel\GstD1

General Information

Symbol

Dmel\GstD1

Species

D. melanogaster

Name

Glutathione S transferase D1

Annotation Symbol

CG10045

Feature Type

protein_coding_gene

FlyBase ID

FBgn0001149

Gene Model Status

Current

Stock Availability

7 publicly available

Enzyme Name (EC)

glutathione peroxidase (1.11.1.9)

glutathione transferase (2.5.1.18)

DDT-dehydrochlorinase (4.5.1.1)

Gene Summary

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:20417639, PubMed:22082028). Has DDT dehydrochlorinase activity (PubMed:20417639). May be involved in detoxification (PubMed:22082028). (UniProt, P20432)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

GST, gstD, DmGSTD1, DmGST1, DmGSTD1-1

Key Links

Genomic Location

Cytogenetic map

87B8-87B8

Sequence location

3R:12,367,817..12,369,268 [-]

Recombination map

3-52

RefSeq locus

NT_033777 REGION:12367817..12369268

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables DDT-dehydrochlorinase activity

inferred from direct assay

(Low et al., 2010)

enables glutathione peroxidase activity

inferred from direct assay

(Saisawang et al., 2012, Tang and Tu, 1994)

enables glutathione transferase activity

inferred from direct assay

(Saisawang et al., 2012, Sawicki et al., 2003, Tang and Tu, 1994, Cochrane and Leblanc, 1986)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables glutathione transferase activity

inferred from biological aspect of ancestor with PANTHER:PTN001679864

(GO Reference Genome Project, 2011-)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in glutathione metabolic process

inferred from direct assay

(Saisawang et al., 2012)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in glutathione metabolic process

inferred from biological aspect of ancestor with PANTHER:PTN001679864

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in cytoplasm

inferred from sequence or structural similarity with RGD:70952

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

SOLUBLE GLUTATHIONE S-TRANSFERASES

CARBON-HALIDE LYASES

GLUTATHIONE PEROXIDASES

Protein Family (UniProt)

Belongs to the GST superfamily. Delta family. (P20432)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

glutathione transferase activity

RX + glutathione = an S-substituted glutathione + a halide anion + H(+) (2.5.1.18)

DDT-dehydrochlorinase activity

1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane = 1,1-dichloro-2,2- bis(4-chlorophenyl)ethylene + chloride + H(+) (4.5.1.1)

RHEA 19217: glutathione peroxidase activity

2 glutathione + H2O2 = glutathione disulfide + 2 H2O (1.11.1.9)

RHEA 16833:

Summaries

Gene Group (FlyBase)

SOLUBLE GLUTATHIONE S-TRANSFERASES -: Glutathione transferases (glutathione S-transferases, GST) catalyze nucleophilic attack by reduced glutathione on nonpolar compounds that contain an electrophilic carbon, nitrogen, or sulfur atom. At least 14 classes of soluble GSTs have been identified in prokaryotes and eukaryotes: Theta, Omega, Sigma and Zeta exist in all eukaryotes. D. melanogaster has two additional classes, Delta and Epsilon. In D. mel, the class is indicated by the letter after the Gst prefix. (Adapted from FBrf0217315 and PMID:15822171).
CARBON-HALIDE LYASES -: Carbon-Halide lyases catalyze the cleavage of a bond between carbon and any halogen atom with the elimination of chloride. (Adapted from FBrf0238856).
GLUTATHIONE PEROXIDASES -: Glutathione peroxidases are oxidoreductases that effectively reduces hydrogen peroxide and lipid peroxides to water and lipid alcohols, respectively, and in turn oxidizes glutathione to glutathione disulfide. (Adapted from PMID:20981034).

Protein Function (UniProtKB)

(UniProt, P20432)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\GstD1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P20432)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

3EIN
3MAK

Comments on Gene Model

Gene model reviewed during 5.53

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

GstD1-RA

FBtr0082607

NM_079602

825

209

GstD1-RB

FBtr0100410

NM_001038953

843

209

Additional Transcript Data and Comments

Reported size (kB)

0.764 (longest cDNA)

(Toung et al., 1990)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

GstD1-PA

FBpp0082077

23.9

209

7.33

FBpp0099824

23.9

209

7.33

P20432

NP_001034042

ABC66168

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

209 aa isoforms: GstD1-PA, GstD1-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

209 (aa)

(Toung et al., 1993, Toung et al., 1991)

209 (aa); 24 (kD predicted)

(Toung et al., 1990)

Comments

External Data

Subunit Structure (UniProtKB)

Homodimer.

(UniProt, P20432)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

3EIN
3MAK

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P20432

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\GstD1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

GstD1_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.94

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

Comment: rapidly degraded

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

embryonic stage 11

posterior midgut primordium

(Nakamura et al., 1999)

anterior midgut primordium

(Nakamura et al., 1999)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

embryonic stage 12 -- 15

midgut primordium | restricted

Comment: stripe of expression

(Nakamura et al., 1999)

embryonic stage 14 -- 15

proventriculus primordium

(Nakamura et al., 1999)

embryonic stage 15

proventriculus inner layer primordium

(Nakamura et al., 1999)

epithelium of embryonic hindgut | anterior

(Nakamura et al., 1999)

epithelium of embryonic/larval midgut primordium | restricted

(Nakamura et al., 1999)

embryonic stage 16

embryonic hindgut | restricted

Comment: point where Malpighian tubules open into hindgut

(Nakamura et al., 1999)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

(Toung et al., 1993)

Additional Descriptive Data

GstD1 is the most abundant Gst mRNA in adults.

(Toung et al., 1993)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\GstD1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\GstD1

View exonic expression by tissue for Dmel\GstD1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001149

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41503/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001149

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001149

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001149

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10045

Flygut - An atlas of the Drosophila adult midgut

FBgn0001149

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\GstD1

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of GstD1

Transgenic constructs containing regulatory region of GstD1

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(3R)Exel6164

(Ryder, 2004.4.26)

Df(3R)Exel6165

(Ryder, 2004.4.26)

Df(3R)Exel7314

(Ryder, 2004.4.26)

Inferred from location ( 12 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Scer\GAL4^pnr-MD237

GstD1^GD6846

Phenotype manifest in

Allele

egg chamber, with Scer\GAL4^GR1

GstD1^HMS05925

nurse cell, with Scer\GAL4^GR1

GstD1^HMS05925

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (27)

Hsap\GSTT2B

8 of 14

Yes

6 of 14

6 of 14

5 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (34)

Rnor\Gstt1

8 of 14

Yes

Rnor\Gstt3

8 of 14

Yes

Rnor\Gstt4

8 of 14

Yes

7 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\RGD1562107

1 of 14

Mus musculus (laboratory mouse) (26)

Mmus\Gstt4

8 of 14

Yes

7 of 14

7 of 14

7 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (17)

Xtro\gstt1-like.2

4 of 13

Yes

3 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (23)

Drer\gstt2

7 of 14

Yes

Drer\gstt1a

6 of 14

Drer\gstt1b

6 of 14

Drer\gstr

5 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (46)

Cele\gst-24

3 of 14

Yes

Cele\gst-43

3 of 14

Yes

Cele\Y53G8B.1

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (36)

Agam\GST1D_ANOGA

10 of 12

Yes

Agam\GSTD11

8 of 12

Yes

Agam\AgaP_AGAP004172

7 of 12

Yes

Agam\AgaP_AGAP012702

7 of 12

Yes

Agam\AgaP_AGAP012838

7 of 12

Yes

Agam\GSTD3

7 of 12

Yes

Agam\GSTD4

7 of 12

Yes

Agam\GSTD5

7 of 12

Yes

Agam\GSTD6

7 of 12

Yes

Agam\GSTD8

7 of 12

Yes

Agam\GSTD10

7 of 12

Yes

Agam\GSTD12

7 of 12

Yes

Agam\GSTT2_ANOGA

7 of 12

Yes

Agam\GSTU2

7 of 12

Yes

Agam\GSTU3

7 of 12

Yes

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

5 of 12

5 of 12

4 of 12

Yes

4 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (58)

Atha\GSTF2

9 of 13

Yes

Atha\GSTF3

9 of 13

Yes

Atha\GSTF5

9 of 13

Yes

Atha\GSTF6

9 of 13

Yes

Atha\GSTF7

9 of 13

Yes

8 of 13

8 of 13

8 of 13

8 of 13

Yes

8 of 13

7 of 13

7 of 13

7 of 13

Yes

6 of 13

6 of 13

6 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

Yes

Atha\AT2G40660

1 of 13

Atha\AT3G47680

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\URE2

8 of 13

Yes

4 of 13

3 of 13

3 of 13

3 of 13

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (7)

Spom\gst1

9 of 12

Yes

Spom\gst2

9 of 12

Yes

Spom\gst3

2 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (9)

Ecol\yfcG

7 of 11

Yes

Ecol\yghU

7 of 11

Yes

5 of 11

3 of 11

2 of 11

1 of 11

Yes

Ecol\gstA

1 of 11

Ecol\yibF

1 of 11

Ecol\yncG

1 of 11

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:GstD1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (43)

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

9 of 13

9 of 13

7 of 13

7 of 13

7 of 13

7 of 13

6 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

GSTT2B; glutathione S-transferase theta 2B

8 of 14

GSTT2; glutathione S-transferase theta 2 (gene/pseudogene)

6 of 14

600437

GSTT4; glutathione S-transferase theta 4

6 of 14

GSTT1; glutathione S-transferase theta 1

5 of 14

600436

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

GstD1

two hybrid

(Hu et al., 2017.6.13)

GstD1 - CycG

two hybrid

(Hu et al., 2017.6.13)

GstD1 - Dus1

two hybrid

(Hu et al., 2017.6.13)

GstD1 - GstD10

experimental knowledge based, two hybrid

(Hu et al., 2017.6.13, Guruharsha et al., 2011)

GstD1 - GstD2

experimental knowledge based

(Guruharsha et al., 2011)

GstD1 - GstD4

experimental knowledge based

(Guruharsha et al., 2011)

GstD1 - GstD5

experimental knowledge based

(Guruharsha et al., 2011)

GstD1 - GstD7

two hybrid, experimental knowledge based

(Hu et al., 2017.6.13, Guruharsha et al., 2011)

GstD1 - GstD8

two hybrid

(Hu et al., 2017.6.13)

GstD1 - GstD9

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Homodimer.

(UniProt, P20432 )

Linkouts

BioGRID - A database of protein and genetic interactions.

66612

DroID - A comprehensive database of gene and protein interactions.

FBgn0001149

MIST (protein-protein) - An integrated Molecular Interaction Database

41503

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-52

Cytogenetic map

87B8-87B8

Sequence location

3R:12,367,817..12,369,268 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

87B8-87B8

Limits computationally determined from genome sequence between P{PZ}svp⁰⁷⁸⁴² and P{lacW}Vha55^j2E9

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

87B-87B

(determined by in situ hybridisation)

(Toung and Tu, 1992, Toung et al., 1991)

87B8-87B9

(determined by in situ hybridisation)

(Toung et al., 1990)

Experimentally Determined Recombination Data

Location

3-52

(FlyBase, 2016)

3-49.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

26926

y¹ w^*; P{EP}GstD1^G15553

77338

y¹ sc^* v¹ sev²¹; P{TRiP.HMS05925}attP40

36818

y¹ sc^* v¹ sev²¹; P{TRiP.GL01039}attP2/TM3, Sb¹

10045R-4

10045R-6

v103246

v17969

w¹¹¹⁸ P{GD6846}v17969

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (191)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RH47312

Other clones

DPiM_RH47312

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0001149

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0001149

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Quantifying rates of protein sequence divergence within and between species reveals that the Drosophila genome harbors a substantial proportion of genes with a very high divergence rate.

(Schmid and Tautz, 1997)

mRNA levels for GST do not increase at adult day 5 in strain showing extended longevity phenotype (ELP).

(Dudas and Arking, 1995)

Glutathione S transferase activity has been analysed in various developmental stages.

(Hunaiti et al., 1995)

Pentobarbital rapidly induces the expression of GstD1, by activating transcription.

(Tang and Tu, 1995)

Dehydro-chlorinase activity of the protein is determined by biochemical analysis using gas-liquid chromatography to assess the contribution of the isozymes to the DDT-resistant phenotype.

(Tang and Tu, 1994)

Encodes glutathione S transferase subunit 1 (Toung et al., Biochem. biophys. Res. Commun. 178: 1205--1211, Toung and Tu, Biochem. biophys. Res. Commun. 182: 355--360).

(Toung et al., 1993)

GstD1 locus is flanked by two clusters of heat shock genes. Enzyme activity in Kc_o cells is elevated slightly to two fold under heat shock.

(Toung et al., 1991)

The GstD1 gene function has been implicated in repairing cellular damage inflicted by oxygen free radicals.

(Lloyd et al., 1990)

Glutathione S-transferase enzyme has been purified and characterised.

(Cochrane et al., 1987)

Glutathione S-transferase activity shows differences in age dependence, substrate selectivity and subcellular location in adult Drosophila, suggesting the presence of multiple forms of the enzyme. Differences in activity between different Drosophila strains are also seen.

(Ottea et al., 1987)

Substrate specificities and pH optima suggest that GstD1 enzyme has a restricted range of substrates. Pentamethyl benzene (PMB) causes a 50% increase in enzyme activity, whereas β,naphthoflavone and phenobarbital have no effect. The degree of response to PMB is modulated by other genes on chromosome 2. Response of enzyme activity to 1-chloro-2,3-dinitrobenzene (CDNB) and ethacrynic acid (EA) can also differ.

(Cochrane and Leblanc, 1986)

GstD1 gene product does not conjugate the epoxides styrene-7,8-oxide and 1,2-epoxy-3-propane.

(Jansen et al., 1984)

Glutathione S-transferase activity has been investigated in sub-cellular fractions of D.melanogaster.

(Baars et al., 1979)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (41)

Reported As

Symbol Synonym

CG10045

(Murari et al., 2022, Çiçek et al., 2016, Rosa-Ferreira et al., 2015, Cammarato et al., 2011, Ling and Salvaterra, 2011, Ni et al., 2011.7.19, Sun et al., 2010, Thornton et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009, Alias and Clark, 2007, Marco et al., 2004, Vierstraete et al., 2004)

(Alias and Clark, 2007)

DmGST-1

(Chelvanayagam et al., 2001, Beall et al., 1992)

DmGST1

(Ranson et al., 2001, Lougarre et al., 1999, Morton, 1993, Murray et al., 1993, Toung et al., 1993)

DmGSTD1

(Schwartz et al., 2024, Wongtrakul et al., 2012, Low et al., 2010, Sawicki et al., 2003, Zimniak and Benes, 2002.7.15, Chelvanayagam et al., 2001)

DmGSTD1-1

(Sharma et al., 2004, Sawicki et al., 2002, Singh et al., 2002)

DmGst1

(Toung et al., 1991)

G-S-T

(Tautz and Schmid, 1998)

GST

(Hultmark and Andó, 2022, Yi et al., 2021, Rajak et al., 2017, Lozinsky et al., 2013, Arking, 1998, Arking et al., 1996, Harshman et al., 1991, Ottea et al., 1987)

GST-1

(Agianian et al., 2003, Singh et al., 2002, Clayton et al., 1998)

GST-D1

(Wagner et al., 2009)

GST1

(Le Goff et al., 2001)

GST1-1

(Pearson, 1996, Toung et al., 1990)

GSTD1

(Kongsung et al., 2024, Schwartz et al., 2024, Zhang and Zhang, 2019, Vorojeikina et al., 2017, Saisawang and Ketterman, 2014, Low et al., 2010, Tohyama and Yamaguchi, 2010, Hooven et al., 2009, Li et al., 2008, Low et al., 2008, Alias and Clark, 2007, Low et al., 2007, Wang et al., 2003, Zou et al., 2000)

GSTase

(Cochrane et al., 1987)

GSt1-1

(Akaboshi et al., 1994)

Gst

(Johnson et al., 2021, Ranz et al., 2001, Ranz et al., 2001, Faridwajidi, 1993, Faridwajidi and Jowett, 1993, Lloyd et al., 1990)

Gst-D1

(Nakamura et al., 1999)

Gst1

(Ke and Hsu, 2019, Toung et al., 1991)

Gst1-1

(Schmid and Tautz, 1997)

Gst1.1

GstD

(Jiang et al., 2022, Joy et al., 2021, Kanwal et al., 2021, Saikumar et al., 2020, Kanda et al., 2011)

GstD1

(Bandyadka et al., 2025, Chaturvedi et al., 2025, Haas et al., 2025, Antosyuk et al., 2024, Chen et al., 2024, Chen et al., 2024, Kim et al., 2024, Petiot et al., 2024, Salminen et al., 2024, Scharenbrock et al., 2024, Clemente and Weavers, 2023, Ito et al., 2023, Shu et al., 2023, Baumgartner et al., 2022, Carlson et al., 2022, Gonis et al., 2022, Känel et al., 2022, Liu et al., 2022, Sebastian et al., 2022, Yan et al., 2022, Yin et al., 2022, Baumgartner et al., 2021, Cattenoz et al., 2021, Jo et al., 2021, Lindsey et al., 2021, Michki et al., 2021, Aboukilila et al., 2020, Brown et al., 2020, Hope et al., 2020, Nakamura et al., 2020, Olufs et al., 2020, Raghuraman et al., 2020, Swanson et al., 2020, West et al., 2020, Darby et al., 2019, Ji et al., 2019, Li et al., 2019, Nash et al., 2019, Reedy et al., 2019, Rodriguez-Fernandez et al., 2019, Sanchez et al., 2019, Baeg et al., 2018, Lin et al., 2018, Sakakibara et al., 2018, Stapper and Jahn, 2018, Tan et al., 2018, Brookheart et al., 2017, Cannavò et al., 2017, Hu et al., 2017.6.13, Jo and Imm, 2017, Lashmanova et al., 2017, Elgart et al., 2016, Khoshnood et al., 2016, Rathore et al., 2016, Calap-Quintana et al., 2015, Mensah et al., 2015, Moskalev et al., 2015, Zhan et al., 2015, Deng and Kerppola, 2014, DeSalvo et al., 2014, Gan et al., 2014, Gloss et al., 2014, Harrop et al., 2014, Laslo et al., 2013, Southall et al., 2013, Awofala et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Boyd et al., 2011, Chahine and O'Donnell, 2011, Ling and Salvaterra, 2011, Anholt and Williams, 2010, Kong et al., 2010, Sun et al., 2010, Roeder et al., 2009, Allen and Spradling, 2008, Gilchrist et al., 2008, Zhang et al., 2007, Morozova et al., 2006)

(Vierstraete et al., 2004)

dGSTT2

(Murari et al., 2022)

dGstD1

(Lee et al., 2021)

dmGSTD1

(Low et al., 2010)

gst

(Chen et al., 2020)

gst-D

(Milton et al., 2011)

gst-D1-1

(Singh et al., 2000)

gstD

(Hirooka et al., 2025, Xu et al., 2024, Louradour et al., 2017, Tsakiri et al., 2017, Muliyil and Narasimha, 2014, Nagy et al., 2013, Tuxworth et al., 2011)

gstd1

(Yuan et al., 2025, Catalani et al., 2024, Cervia et al., 2024, Catalani et al., 2023, Saisawang et al., 2012, Wayne et al., 2011)

Name Synonyms

CG10045 Glutathione S-transferase

(Kang et al., 2002)

Glutathione S transferase D

(Mensah et al., 2015)

Glutathione S transferase D1

(Zhan et al., 2015, Cammarato et al., 2011, Kanda et al., 2011, Varma and Mishra, 2011)

Glutathione S-transferase 1

(Pearson, 1998)

Glutathione S-transferase D1

(Sun et al., 2007)

Glutathione S-transferase-D1

(Nakamura et al., 1999)

Glutathione-S-transferase 1

(Ling and Salvaterra, 2011)

glutathione S-transferase D1

(Mensah et al., 2015, Muliyil and Narasimha, 2014, Boyd et al., 2011, Rand et al., 2009)

Secondary FlyBase IDs

FBgn0010045

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 65 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

41503

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P20432

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P20432

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P20432

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001149

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41503/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001149

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001149

FlyMine - An integrated database for Drosophila genomics

FBgn0001149

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG10045

MARRVEL_MODEL - MARRVEL (model organism gene)

41503

PDB - An information portal to biological macromolecular structures

3EIN
3MAK

Linkouts

BioGRID - A database of protein and genetic interactions.

66612

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0001149

DroID - A comprehensive database of gene and protein interactions.

FBgn0001149

DRSC - Results frm RNAi screens

FBgn0001149

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

GstD1_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001149

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0001149

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10045

Flygut - An atlas of the Drosophila adult midgut

FBgn0001149

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

41503

References (276)

The following external sites may use different assemblies or annotations than FlyBase.

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