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Gene Dmel\GstD1

General Information
Symbol	Dmel\GstD1	Species	D. melanogaster
Name	Glutathione S transferase D1	Annotation symbol	CG10045
Feature type	protein_coding_gene	FlyBase ID	FBgn0001149
Gene Model Status	Current	Stock availability	5 publicly available
Also Known As	Gst, DmGST1, GST-1, DmGSTD1, Gst1-1, DmGSTD1-1, DmGST-1, GST1, GST-D1
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	87B8-87B8	Sequence location	3R:8,193,269..8,194,987 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Glutathione S transferase D1 is referred to in FlyBase by the symbol Dmel\GstD1 (CG10045, FBgn0001149). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: DDT-dehydrochlorinase activity; glutathione transferase activity. There is experimental evidence that it is involved in the biological process: glutathione metabolic process. 11 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Glutathione S-transferase, C-terminal; Glutathione S-transferase, C-terminal-like; Glutathione S-transferase, N-terminal; Glutathione S-transferase/chloride channel, C-terminal; Thioredoxin-like fold. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1628353_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of GstD1. Gene sequence location is 3R:8193269..8194987.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Controlled Vocabulary Terms cytoplasm
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 87B8-87B8   Limits computationally determined from genome sequence between P{PZ}svp07842 and P{lacW}Vha55j2E9
Experimentally Determined Cytological Location
Cytogenetic map Notes References 87B-87B   (determined by in situ hybridisation)   (Toung and Tu, 1992) 87B-87B   (determined by in situ hybridisation)   (Toung et al., 1991) 87B8-87B9   (determined by in situ hybridisation)   (Toung et al., 1990)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\GstD1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) GstD1-RA FBtr0082607  NM_079602   822   209 GstD1-RB FBtr0100410  NM_001038953   1149   209
Additional Transcript Data & Comments
Reported size (kB)	0.764 (longest cDNA) (Toung et al., 1990)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein GstD1-PA   FBpp0082077   23.9   209   7.33     NP_524326   AAF54786 GstD1-PB   FBpp0099824   23.9   209   7.33     NP_001034042   ABC66168
Additional Polypeptide Data & Comments
Reported size (kDa)	209 (aa); 24 (kD predicted) (Toung et al., 1990) 209 (aa) (Toung et al., 1993, Toung et al., 1991)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Glutathione S-transferase, N-terminal (IPR004045) Glutathione S-transferase, C-terminal (IPR004046) Glutathione S-transferase, C-terminal-like (IPR010987) Thioredoxin-like fold (IPR012336) Glutathione S-transferase/chloride channel, C-terminal (IPR017933)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA440129   AA735823   AA803543   AA821086   AE014297 AAF54786   AE014297 ABC66168   AI063567   AI063572   AI106741   AI108593   AI114250   AI237926   AI257606   AI293639   AI297266   AI297287   AI297766   AI297879   AI386700   AI402955   AI404482   AI404652   AI405136   AI405646   AI945315   AI946965   AY121705 AAM52032   AY231942   BE975707   BE975708   BE977984   BE978635   BG633323   BG639457   BI166232   BI166856   BI173431   BI173616   BI213626   BI237061   BI238266   BI352575   BI353076   BI354100   BI355017   BI355793   BI357499   BI370266   BI482418   BI482515   BI484677   BI484784   BI485678   BI563703   BI563792   BI563953   BI565138   BI565541   BI566816   BI570733   BI571145   BI571169   BI572719   BI572733   BI573998   BI573999   BI574228   BI578985   BI583977   BI584943   BI586842   BI590866   BI604676   BI608103   BI612130   BI612879   BI617087   BI623789   BI625973   BI626733   BI629634   BI631598   BI634629   BP541885   BP544840   BP544841   BP546841   BP553235   BP554729   BP554730   BP557196   BP559624   BP559625   BQ103115   BT023393   BT030973   BT132814   BX007504   BX048001   BX048902   BX049011   BX050759   BX053230   BX057827   BX059599   CA804599   CA804672   CK658435   CK660955   CO154293   CO154717   CO155352   CO155494   CO157108   CO181086   CO182114   CO184508   CO184907   CO185084   CO192047   CO192567   CO195447   CO268026   CO270030   CO270166   CO276157   CO308155   CO314771   CO336825   CO337139   CO337242   CO338494   CO338843   CR938483   EC059545   EC065235   EC071260   EC075652   EC077644   EC088328   EC208709   EC220184   EC220333   EC226265   EC261314   EC262006   EC262929   EC264265   EC265170   EC265362   EC265607   EC266036   EC267142   EC267372   EV575116   EV575654   EV575776   EV576524   EV576652   EV577044   EV577315   EV578576   EV578967   EV581003   EV581321   EV582190   EV582355   EV584401   EV585651   EV585794   EV592199   EV593605   EV596767   EV597185   EV599102   EV599879   EV599914   GH756391   GH757890   GH774189   GH781017   GH792641   GH798444   GH804943   GH808055   GH821441   GH827533   GH829494   GH833809   GH837168   GH838998   GH840311   GH843290   GH847243   GH848916   GH855644   GH860516   GH860958   GH861000   GH871692   GH880679   GH881014   GH884665   GH885508   GH896211   GH896373   GH897284   GH902629   GH904602   GH908681   GH920674   GH920961   GH922036   GH933221   GH934140   M97702   S51044   X14233 CAA32449
UniProtKB/Swiss-Prot	P20432
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) Comment:rapidly degraded embryonic stage 9 -- 10   anterior endoderm   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) embryonic stage 11   posterior midgut primordium   (Nakamura et al., 1999) anterior midgut primordium   (Nakamura et al., 1999) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) embryonic stage 12 -- 15   midgut primordium | restricted   (Nakamura et al., 1999) Comment:stripe of expression embryonic stage 13 -- 16   dorsal sensory complex proper primordium   (Fisher et al., 2012) Comment:reported as dorsal/lateral sensory complexes embryonic head sensory system   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic proventriculus   (Fisher et al., 2012) embryonic proventriculus inner layer   (Fisher et al., 2012) embryonic proventriculus intermediate layer   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) sensory nervous system primordium   (Fisher et al., 2012) ventral sensory complex primordium   (Fisher et al., 2012) embryonic stage 14 -- 15   proventriculus primordium   (Nakamura et al., 1999) embryonic stage 15   proventriculus inner layer primordium   (Nakamura et al., 1999) epithelium of embryonic hindgut | anterior   (Nakamura et al., 1999) epithelium of embryonic/larval midgut primordium | restricted   (Nakamura et al., 1999) embryonic stage 16   embryonic hindgut | restricted   (Nakamura et al., 1999) Comment:point where Malpighian tubules open into hindgut northern blot Stage Tissue/Position (including subcellular localization) Reference adult stage     (Toung et al., 1993)
Additional Descriptive Data
	GstD1 is the most abundant Gst mRNA in adults. (Toung et al., 1993)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1462.775 Larval Midgut    1832.6 Larval Hindgut    3499.6 Larval Malpighian Tubules    4359.7 Larval Fat Body    4926.7 Larval Salivary Gland    8435.7 Larval Trachea    4977.8 Larval Carcass    4100.95 Adult Head    2603.1 Adult Eye    1569.075 Adult Brain    1727.6 Adult Thoracic-Abdominal Ganglion    2109.1 Adult Crop    4093.8 Adult Midgut    3151 Adult Hindgut    3481.1 Adult Malpighian Tubules    4079.5 Adult Fat Body    1241.4 Adult Salivary Gland    2372.1 Adult Heart    2356.925 Adult VirginFemale Spermatheca    3220.8 Adult InseminatedFemale Spermatheca    3217.4 Adult Ovary    2887.9 Adult Testis    1885.6 Adult Male Accessory Gland    3478.1 Adult Carcass    2692.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1462.775) Larval Midgut  (1832.6) Larval Hindgut  (3499.6) Larval Malpighian Tubules  (4359.7) Larval Fat Body  (4926.7) Larval Salivary Gland  (8435.7) Larval Trachea  (4977.8) Larval Carcass  (4100.95) Adult Head  (2603.1) Adult Eye  (1569.075) Adult Brain  (1727.6) Adult Thoracic-Abdominal Ganglion  (2109.1) Adult Crop  (4093.8) Adult Midgut  (3151) Adult Hindgut  (3481.1) Adult Malpighian Tubules  (4079.5) Adult Fat Body  (1241.4) Adult Salivary Gland  (2372.1) Adult Heart  (2356.925) Adult VirginFemale Spermatheca  (3220.8) Adult InseminatedFemale Spermatheca  (3217.4) Adult Ovary  (2887.9) Adult Testis  (1885.6) Adult Male Accessory Gland  (3478.1) Adult Carcass  (2692.8) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (1462.775) Larval Midgut  (1832.6) Larval Hindgut  (3499.6) Larval Malpighian Tubules  (4359.7) Larval Fat Body  (4926.7) Larval Salivary Gland  (8435.7) Larval Trachea  (4977.8) Larval Carcass  (4100.95) Adult Head  (2603.1) Adult Eye  (1569.075) Adult Brain  (1727.6) Adult Thoracic-Abdominal Ganglion  (2109.1) Adult Crop  (4093.8) Adult Midgut  (3151) Adult Hindgut  (3481.1) Adult Malpighian Tubules  (4079.5) Adult Fat Body  (1241.4) Adult Salivary Gland  (2372.1) Adult Heart  (2356.925) Adult VirginFemale Spermatheca  (3220.8) Adult InseminatedFemale Spermatheca  (3217.4) Adult Ovary  (2887.9) Adult Testis  (1885.6) Adult Male Accessory Gland  (3478.1) Adult Carcass  (2692.8) Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1462.775 Larval Midgut    1832.6 Larval Hindgut    3499.6 Larval Malpighian Tubules    4359.7 Larval Fat Body    4926.7 Larval Salivary Gland    8435.7 Larval Trachea    4977.8 Larval Carcass    4100.95 Adult Head    2603.1 Adult Eye    1569.075 Adult Brain    1727.6 Adult Thoracic-Abdominal Ganglion    2109.1 Adult Crop    4093.8 Adult Midgut    3151 Adult Hindgut    3481.1 Adult Malpighian Tubules    4079.5 Adult Fat Body    1241.4 Adult Salivary Gland    2372.1 Adult Heart    2356.925 Adult VirginFemale Spermatheca    3220.8 Adult InseminatedFemale Spermatheca    3217.4 Adult Ovary    2887.9 Adult Testis    1885.6 Adult Male Accessory Gland    3478.1 Adult Carcass    2692.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1462.775 Larval Midgut    1832.6 Larval Hindgut    3499.6 Larval Malpighian Tubules    4359.7 Larval Fat Body    4926.7 Larval Salivary Gland    8435.7 Larval Trachea    4977.8 Larval Carcass    4100.95 Adult Head    2603.1 Adult Eye    1569.075 Adult Brain    1727.6 Adult Thoracic-Abdominal Ganglion    2109.1 Adult Crop    4093.8 Adult Midgut    3151 Adult Hindgut    3481.1 Adult Malpighian Tubules    4079.5 Adult Fat Body    1241.4 Adult Salivary Gland    2372.1 Adult Heart    2356.925 Adult VirginFemale Spermatheca    3220.8 Adult InseminatedFemale Spermatheca    3217.4 Adult Ovary    2887.9 Adult Testis    1885.6 Adult Male Accessory Gland    3478.1 Adult Carcass    2692.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1462.775) Larval Midgut  (1832.6) Larval Hindgut  (3499.6) Larval Malpighian Tubules  (4359.7) Larval Fat Body  (4926.7) Larval Salivary Gland  (8435.7) Larval Trachea  (4977.8) Larval Carcass  (4100.95) Adult Head  (2603.1) Adult Eye  (1569.075) Adult Brain  (1727.6) Adult Thoracic-Abdominal Ganglion  (2109.1) Adult Crop  (4093.8) Adult Midgut  (3151) Adult Hindgut  (3481.1) Adult Malpighian Tubules  (4079.5) Adult Fat Body  (1241.4) Adult Salivary Gland  (2372.1) Adult Heart  (2356.925) Adult VirginFemale Spermatheca  (3220.8) Adult InseminatedFemale Spermatheca  (3217.4) Adult Ovary  (2887.9) Adult Testis  (1885.6) Adult Male Accessory Gland  (3478.1) Adult Carcass  (2692.8) Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1462.775 Larval Midgut  (1832.6) Larval Hindgut  (3499.6) Larval Malpighian Tubules  (4359.7) Larval Fat Body  (4926.7) Larval Salivary Gland  (8435.7) Larval Trachea  (4977.8) Larval Carcass  (4100.95) Adult Head  (2603.1) Adult Eye  1569.075 Adult Brain  (1727.6) Adult Thoracic-Abdominal Ganglion  (2109.1) Adult Crop  (4093.8) Adult Midgut  (3151) Adult Hindgut  (3481.1) Adult Malpighian Tubules  (4079.5) Adult Fat Body  1241.4 Adult Salivary Gland  (2372.1) Adult Heart  (2356.925) Adult VirginFemale Spermatheca  (3220.8) Adult InseminatedFemale Spermatheca  (3217.4) Adult Ovary  (2887.9) Adult Testis  (1885.6) Adult Male Accessory Gland  (3478.1) Adult Carcass  (2692.8) Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1462.775 Larval Midgut    1832.6 Larval Hindgut    3499.6 Larval Malpighian Tubules    4359.7 Larval Fat Body    4926.7 Larval Salivary Gland    8435.7 Larval Trachea    4977.8 Larval Carcass    4100.95 Adult Head    2603.1 Adult Eye    1569.075 Adult Brain    1727.6 Adult Thoracic-Abdominal Ganglion    2109.1 Adult Crop    4093.8 Adult Midgut    3151 Adult Hindgut    3481.1 Adult Malpighian Tubules    4079.5 Adult Fat Body    1241.4 Adult Salivary Gland    2372.1 Adult Heart    2356.925 Adult VirginFemale Spermatheca    3220.8 Adult InseminatedFemale Spermatheca    3217.4 Adult Ovary    2887.9 Adult Testis    1885.6 Adult Male Accessory Gland    3478.1 Adult Carcass    2692.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    2603.1   NA  Eye    1569.075   NA  Brain    1727.6   1462.775  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    2109.1   NA  Crop    4093.8   1832.6  Midgut    3151   3499.6  Hindgut    3481.1   4359.7  Malpighian Tubules    4079.5   4926.7  Fat Body    1241.4   8435.7  Salivary Gland    2372.1   NA  Heart    2356.925   4977.8  Trachea    NA   NA  VirginFemale Spermatheca    3220.8   NA  InseminatedFemale Spermatheca    3217.4   NA  Ovary    2887.9   NA  Testis    1885.6   NA  Male Accessory Gland    3478.1   4100.95  Carcass    2692.8 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    258 central nervous system, larvae L3    50 central nervous system, pupae P8    47 head, virgin 1-day female    54 head, virgin 4-day female    60 head, virgin 20-day female    93 head, mated 1-day female    49 head, mated 4-day female    46 head, mated 20-day female    85 head, mated 1-day male    74 head, mated 4-day male    87 head, mated 20-day male    138 salivary gland, larvae L3 wandering    35 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    137 digestive system, 1-day adult    527 digestive system, 4-day adult    284 digestive system, 20-day adult    396 fat body, larvae L3 wandering    137 fat body, white prepupae    87 fat body, pupae P8    79 carcass, larvae L3 wandering    144 carcass, 1-day adult    102 carcass, 4-day adult    93 carcass, 20-day adult    173 ovary, virgin 4-day female    289 ovary, mated 4-day female    199 testis, mated 4-day male    193 accessory gland, mated 4-day male    166 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (258) central nervous system, larvae L3  (50) central nervous system, pupae P8  (47) head, virgin 1-day female  (54) head, virgin 4-day female  (60) head, virgin 20-day female  (93) head, mated 1-day female  (49) head, mated 4-day female  (46) head, mated 20-day female  (85) head, mated 1-day male  (74) head, mated 4-day male  (87) head, mated 20-day male  (138) salivary gland, larvae L3 wandering  (35) salivary gland, white prepupae    25 digestive system, larvae L3 wandering  (137) digestive system, 1-day adult  (527) digestive system, 4-day adult  (284) digestive system, 20-day adult  (396) fat body, larvae L3 wandering  (137) fat body, white prepupae  (87) fat body, pupae P8  (79) carcass, larvae L3 wandering  (144) carcass, 1-day adult  (102) carcass, 4-day adult  (93) carcass, 20-day adult  (173) ovary, virgin 4-day female  (289) ovary, mated 4-day female  (199) testis, mated 4-day male  (193) accessory gland, mated 4-day male  (166) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (258) central nervous system, larvae L3    50 central nervous system, pupae P8    47 head, virgin 1-day female    54 head, virgin 4-day female    60 head, virgin 20-day female    93 head, mated 1-day female    49 head, mated 4-day female    46 head, mated 20-day female    85 head, mated 1-day male    74 head, mated 4-day male    87 head, mated 20-day male  (138) salivary gland, larvae L3 wandering    35 salivary gland, white prepupae    25 digestive system, larvae L3 wandering  (137) digestive system, 1-day adult  (527) digestive system, 4-day adult  (284) digestive system, 20-day adult  (396) fat body, larvae L3 wandering  (137) fat body, white prepupae    87 fat body, pupae P8    79 carcass, larvae L3 wandering  (144) carcass, 1-day adult  102 carcass, 4-day adult    93 carcass, 20-day adult  (173) ovary, virgin 4-day female  (289) ovary, mated 4-day female  (199) testis, mated 4-day male  (193) accessory gland, mated 4-day male  (166) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    258 central nervous system, larvae L3    50 central nervous system, pupae P8    47 head, virgin 1-day female    54 head, virgin 4-day female    60 head, virgin 20-day female    93 head, mated 1-day female    49 head, mated 4-day female    46 head, mated 20-day female    85 head, mated 1-day male    74 head, mated 4-day male    87 head, mated 20-day male    138 salivary gland, larvae L3 wandering    35 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    137 digestive system, 1-day adult    527 digestive system, 4-day adult    284 digestive system, 20-day adult    396 fat body, larvae L3 wandering    137 fat body, white prepupae    87 fat body, pupae P8    79 carcass, larvae L3 wandering    144 carcass, 1-day adult    102 carcass, 4-day adult    93 carcass, 20-day adult    173 ovary, virgin 4-day female    289 ovary, mated 4-day female    199 testis, mated 4-day male    193 accessory gland, mated 4-day male    166 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    258 central nervous system, larvae L3    50 central nervous system, pupae P8    47 head, virgin 1-day female    54 head, virgin 4-day female    60 head, virgin 20-day female    93 head, mated 1-day female    49 head, mated 4-day female    46 head, mated 20-day female    85 head, mated 1-day male    74 head, mated 4-day male    87 head, mated 20-day male    138 salivary gland, larvae L3 wandering    35 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    137 digestive system, 1-day adult    527 digestive system, 4-day adult    284 digestive system, 20-day adult    396 fat body, larvae L3 wandering    137 fat body, white prepupae    87 fat body, pupae P8    79 carcass, larvae L3 wandering    144 carcass, 1-day adult    102 carcass, 4-day adult    93 carcass, 20-day adult    173 ovary, virgin 4-day female    289 ovary, mated 4-day female    199 testis, mated 4-day male    193 accessory gland, mated 4-day male    166 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (258) central nervous system, larvae L3  (50) central nervous system, pupae P8  (47) head, virgin 1-day female  (54) head, virgin 4-day female  (60) head, virgin 20-day female  (93) head, mated 1-day female  (49) head, mated 4-day female  (46) head, mated 20-day female  (85) head, mated 1-day male  (74) head, mated 4-day male  (87) head, mated 20-day male  (138) salivary gland, larvae L3 wandering  (35) salivary gland, white prepupae    25 digestive system, larvae L3 wandering  (137) digestive system, 1-day adult  (527) digestive system, 4-day adult  (284) digestive system, 20-day adult  (396) fat body, larvae L3 wandering  (137) fat body, white prepupae  (87) fat body, pupae P8  (79) carcass, larvae L3 wandering  (144) carcass, 1-day adult  (102) carcass, 4-day adult  (93) carcass, 20-day adult  (173) ovary, virgin 4-day female  (289) ovary, mated 4-day female  (199) testis, mated 4-day male  (193) accessory gland, mated 4-day male  (166) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (258) central nervous system, larvae L3    50 central nervous system, pupae P8    47 head, virgin 1-day female    54 head, virgin 4-day female    60 head, virgin 20-day female    93 head, mated 1-day female    49 head, mated 4-day female    46 head, mated 20-day female    85 head, mated 1-day male    74 head, mated 4-day male    87 head, mated 20-day male  138 salivary gland, larvae L3 wandering    35 salivary gland, white prepupae    25 digestive system, larvae L3 wandering  137 digestive system, 1-day adult  (527) digestive system, 4-day adult  (284) digestive system, 20-day adult  (396) fat body, larvae L3 wandering  137 fat body, white prepupae    87 fat body, pupae P8    79 carcass, larvae L3 wandering  (144) carcass, 1-day adult  102 carcass, 4-day adult    93 carcass, 20-day adult  (173) ovary, virgin 4-day female  (289) ovary, mated 4-day female  (199) testis, mated 4-day male  (193) accessory gland, mated 4-day male  (166) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    258 central nervous system, larvae L3    50 central nervous system, pupae P8    47 head, virgin 1-day female    54 head, virgin 4-day female    60 head, virgin 20-day female    93 head, mated 1-day female    49 head, mated 4-day female    46 head, mated 20-day female    85 head, mated 1-day male    74 head, mated 4-day male    87 head, mated 20-day male    138 salivary gland, larvae L3 wandering    35 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    137 digestive system, 1-day adult    527 digestive system, 4-day adult    284 digestive system, 20-day adult    396 fat body, larvae L3 wandering    137 fat body, white prepupae    87 fat body, pupae P8    79 carcass, larvae L3 wandering    144 carcass, 1-day adult    102 carcass, 4-day adult    93 carcass, 20-day adult    173 ovary, virgin 4-day female    289 ovary, mated 4-day female    199 testis, mated 4-day male    193 accessory gland, mated 4-day male    166 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    496 embryo 02-04hr    12 embryo 04-06hr    10 embryo 06-08hr    16 embryo 08-10hr    26 embryo 10-12hr    87 embryo 12-14hr    80 embryo 14-16hr    136 embryo 16-18hr    122 embryo 18-20hr    139 embryo 20-22hr    221 embryo 22-24hr    267 larva L1    310 larva L2    570 larva L3 12hr old    665 larva L3 puffstage 1-2    428 larva L3 puffstage 3-6    310 larva L3 puffstage 7-9    286 white prepupae new    311 white prepupae 12hr    231 white prepupae 24hr    339 pupae 2d postWPP    402 pupae 3d postWPP    276 pupae 4d postWPP    211 adult male 01day    267 adult male 05day    233 adult male 30day    282 adult female 01day    297 adult female 05day    396 adult female 30day    396 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (496) embryo 02-04hr    12 embryo 04-06hr    10 embryo 06-08hr    16 embryo 08-10hr  26 embryo 10-12hr  (87) embryo 12-14hr  (80) embryo 14-16hr  (136) embryo 16-18hr  (122) embryo 18-20hr  (139) embryo 20-22hr  (221) embryo 22-24hr  (267) larva L1  (310) larva L2  (570) larva L3 12hr old  (665) larva L3 puffstage 1-2  (428) larva L3 puffstage 3-6  (310) larva L3 puffstage 7-9  (286) white prepupae new  (311) white prepupae 12hr  (231) white prepupae 24hr  (339) pupae 2d postWPP  (402) pupae 3d postWPP  (276) pupae 4d postWPP  (211) adult male 01day  (267) adult male 05day  (233) adult male 30day  (282) adult female 01day  (297) adult female 05day  (396) adult female 30day  (396) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (496) embryo 02-04hr    12 embryo 04-06hr    10 embryo 06-08hr    16 embryo 08-10hr    26 embryo 10-12hr    87 embryo 12-14hr    80 embryo 14-16hr  (136) embryo 16-18hr  (122) embryo 18-20hr  (139) embryo 20-22hr  (221) embryo 22-24hr  (267) larva L1  (310) larva L2  (570) larva L3 12hr old  (665) larva L3 puffstage 1-2  (428) larva L3 puffstage 3-6  (310) larva L3 puffstage 7-9  (286) white prepupae new  (311) white prepupae 12hr  (231) white prepupae 24hr  (339) pupae 2d postWPP  (402) pupae 3d postWPP  (276) pupae 4d postWPP  (211) adult male 01day  (267) adult male 05day  (233) adult male 30day  (282) adult female 01day  (297) adult female 05day  (396) adult female 30day  (396) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    496 embryo 02-04hr    12 embryo 04-06hr    10 embryo 06-08hr    16 embryo 08-10hr    26 embryo 10-12hr    87 embryo 12-14hr    80 embryo 14-16hr    136 embryo 16-18hr    122 embryo 18-20hr    139 embryo 20-22hr    221 embryo 22-24hr    267 larva L1    310 larva L2    570 larva L3 12hr old    665 larva L3 puffstage 1-2    428 larva L3 puffstage 3-6    310 larva L3 puffstage 7-9    286 white prepupae new    311 white prepupae 12hr    231 white prepupae 24hr    339 pupae 2d postWPP    402 pupae 3d postWPP    276 pupae 4d postWPP    211 adult male 01day    267 adult male 05day    233 adult male 30day    282 adult female 01day    297 adult female 05day    396 adult female 30day    396 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    496 embryo 02-04hr    12 embryo 04-06hr    10 embryo 06-08hr    16 embryo 08-10hr    26 embryo 10-12hr    87 embryo 12-14hr    80 embryo 14-16hr    136 embryo 16-18hr    122 embryo 18-20hr    139 embryo 20-22hr    221 embryo 22-24hr    267 larva L1    310 larva L2    570 larva L3 12hr old    665 larva L3 puffstage 1-2    428 larva L3 puffstage 3-6    310 larva L3 puffstage 7-9    286 white prepupae new    311 white prepupae 12hr    231 white prepupae 24hr    339 pupae 2d postWPP    402 pupae 3d postWPP    276 pupae 4d postWPP    211 adult male 01day    267 adult male 05day    233 adult male 30day    282 adult female 01day    297 adult female 05day    396 adult female 30day    396 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (496) embryo 02-04hr    12 embryo 04-06hr    10 embryo 06-08hr    16 embryo 08-10hr  26 embryo 10-12hr  (87) embryo 12-14hr  (80) embryo 14-16hr  (136) embryo 16-18hr  (122) embryo 18-20hr  (139) embryo 20-22hr  (221) embryo 22-24hr  (267) larva L1  (310) larva L2  (570) larva L3 12hr old  (665) larva L3 puffstage 1-2  (428) larva L3 puffstage 3-6  (310) larva L3 puffstage 7-9  (286) white prepupae new  (311) white prepupae 12hr  (231) white prepupae 24hr  (339) pupae 2d postWPP  (402) pupae 3d postWPP  (276) pupae 4d postWPP  (211) adult male 01day  (267) adult male 05day  (233) adult male 30day  (282) adult female 01day  (297) adult female 05day  (396) adult female 30day  (396) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (496) embryo 02-04hr    12 embryo 04-06hr    10 embryo 06-08hr    16 embryo 08-10hr    26 embryo 10-12hr    87 embryo 12-14hr    80 embryo 14-16hr  136 embryo 16-18hr  122 embryo 18-20hr  139 embryo 20-22hr  (221) embryo 22-24hr  (267) larva L1  (310) larva L2  (570) larva L3 12hr old  (665) larva L3 puffstage 1-2  (428) larva L3 puffstage 3-6  (310) larva L3 puffstage 7-9  (286) white prepupae new  (311) white prepupae 12hr  (231) white prepupae 24hr  (339) pupae 2d postWPP  (402) pupae 3d postWPP  (276) pupae 4d postWPP  (211) adult male 01day  (267) adult male 05day  (233) adult male 30day  (282) adult female 01day  (297) adult female 05day  (396) adult female 30day  (396) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    496 embryo 02-04hr    12 embryo 04-06hr    10 embryo 06-08hr    16 embryo 08-10hr    26 embryo 10-12hr    87 embryo 12-14hr    80 embryo 14-16hr    136 embryo 16-18hr    122 embryo 18-20hr    139 embryo 20-22hr    221 embryo 22-24hr    267 larva L1    310 larva L2    570 larva L3 12hr old    665 larva L3 puffstage 1-2    428 larva L3 puffstage 3-6    310 larva L3 puffstage 7-9    286 white prepupae new    311 white prepupae 12hr    231 white prepupae 24hr    339 pupae 2d postWPP    402 pupae 3d postWPP    276 pupae 4d postWPP    211 adult male 01day    267 adult male 05day    233 adult male 30day    282 adult female 01day    297 adult female 05day    396 adult female 30day    396 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    42 Schneider line 2 Sg4    597 embryonic 1182-4H    77 embryonic GM2    245 embryonic Kc167    389 embryonic S1    251 embryonic S3    486 leg disc CME L1    556 wing disc CME-W2    399 wing disc ML-DmD8    59 wing disc ML-DmD9    252 wing disc ML-DmD16-c3    136 wing disc ML-DmD21    133 wing disc ML-DmD32    182 haltere disc ML-DmD17-c3    64 eye-antennal disc ML-DmD11    447 antennal disc ML-DmD20-c5    405 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    323 CNS ML-DmBG2-c2    527 tumorous blood cells mbn2    71 ovary fGS/OSS    78 ovary OSC    54 ovary OSS    45 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (42) Schneider line 2 Sg4  (597) embryonic 1182-4H  (77) embryonic GM2  (245) embryonic Kc167  (389) embryonic S1  (251) embryonic S3  (486) leg disc CME L1  (556) wing disc CME-W2  (399) wing disc ML-DmD8  (59) wing disc ML-DmD9  (252) wing disc ML-DmD16-c3  (136) wing disc ML-DmD21  (133) wing disc ML-DmD32  (182) haltere disc ML-DmD17-c3  (64) eye-antennal disc ML-DmD11  (447) antennal disc ML-DmD20-c5  (405) mixed discs ML-DmD4-c1  (54) CNS ML-DmBG1-c1  (323) CNS ML-DmBG2-c2  (527) tumorous blood cells mbn2  (71) ovary fGS/OSS  (78) ovary OSC  (54) ovary OSS  (45) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    42 Schneider line 2 Sg4  (597) embryonic 1182-4H    77 embryonic GM2  (245) embryonic Kc167  (389) embryonic S1  (251) embryonic S3  (486) leg disc CME L1  (556) wing disc CME-W2  (399) wing disc ML-DmD8    59 wing disc ML-DmD9  (252) wing disc ML-DmD16-c3  (136) wing disc ML-DmD21  (133) wing disc ML-DmD32  (182) haltere disc ML-DmD17-c3    64 eye-antennal disc ML-DmD11  (447) antennal disc ML-DmD20-c5  (405) mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1  (323) CNS ML-DmBG2-c2  (527) tumorous blood cells mbn2    71 ovary fGS/OSS    78 ovary OSC    54 ovary OSS    45 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    42 Schneider line 2 Sg4    597 embryonic 1182-4H    77 embryonic GM2    245 embryonic Kc167    389 embryonic S1    251 embryonic S3    486 leg disc CME L1    556 wing disc CME-W2    399 wing disc ML-DmD8    59 wing disc ML-DmD9    252 wing disc ML-DmD16-c3    136 wing disc ML-DmD21    133 wing disc ML-DmD32    182 haltere disc ML-DmD17-c3    64 eye-antennal disc ML-DmD11    447 antennal disc ML-DmD20-c5    405 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    323 CNS ML-DmBG2-c2    527 tumorous blood cells mbn2    71 ovary fGS/OSS    78 ovary OSC    54 ovary OSS    45 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    42 Schneider line 2 Sg4    597 embryonic 1182-4H    77 embryonic GM2    245 embryonic Kc167    389 embryonic S1    251 embryonic S3    486 leg disc CME L1    556 wing disc CME-W2    399 wing disc ML-DmD8    59 wing disc ML-DmD9    252 wing disc ML-DmD16-c3    136 wing disc ML-DmD21    133 wing disc ML-DmD32    182 haltere disc ML-DmD17-c3    64 eye-antennal disc ML-DmD11    447 antennal disc ML-DmD20-c5    405 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    323 CNS ML-DmBG2-c2    527 tumorous blood cells mbn2    71 ovary fGS/OSS    78 ovary OSC    54 ovary OSS    45 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (42) Schneider line 2 Sg4  (597) embryonic 1182-4H  (77) embryonic GM2  (245) embryonic Kc167  (389) embryonic S1  (251) embryonic S3  (486) leg disc CME L1  (556) wing disc CME-W2  (399) wing disc ML-DmD8  (59) wing disc ML-DmD9  (252) wing disc ML-DmD16-c3  (136) wing disc ML-DmD21  (133) wing disc ML-DmD32  (182) haltere disc ML-DmD17-c3  (64) eye-antennal disc ML-DmD11  (447) antennal disc ML-DmD20-c5  (405) mixed discs ML-DmD4-c1  (54) CNS ML-DmBG1-c1  (323) CNS ML-DmBG2-c2  (527) tumorous blood cells mbn2  (71) ovary fGS/OSS  (78) ovary OSC  (54) ovary OSS  (45) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    42 Schneider line 2 Sg4  (597) embryonic 1182-4H    77 embryonic GM2  (245) embryonic Kc167  (389) embryonic S1  (251) embryonic S3  (486) leg disc CME L1  (556) wing disc CME-W2  (399) wing disc ML-DmD8    59 wing disc ML-DmD9  (252) wing disc ML-DmD16-c3  136 wing disc ML-DmD21  133 wing disc ML-DmD32  (182) haltere disc ML-DmD17-c3    64 eye-antennal disc ML-DmD11  (447) antennal disc ML-DmD20-c5  (405) mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1  (323) CNS ML-DmBG2-c2  (527) tumorous blood cells mbn2    71 ovary fGS/OSS    78 ovary OSC    54 ovary OSS    45 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    42 Schneider line 2 Sg4    597 embryonic 1182-4H    77 embryonic GM2    245 embryonic Kc167    389 embryonic S1    251 embryonic S3    486 leg disc CME L1    556 wing disc CME-W2    399 wing disc ML-DmD8    59 wing disc ML-DmD9    252 wing disc ML-DmD16-c3    136 wing disc ML-DmD21    133 wing disc ML-DmD32    182 haltere disc ML-DmD17-c3    64 eye-antennal disc ML-DmD11    447 antennal disc ML-DmD20-c5    405 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    323 CNS ML-DmBG2-c2    527 tumorous blood cells mbn2    71 ovary fGS/OSS    78 ovary OSC    54 ovary OSS    45 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    88 cold shock, 4-day adult    54 heat shock, 4-day adult    321 Cadmium 50 mM 6 hrs, larvae L3    608 Cadmium 50 mM 12 hrs, larvae L3    289 Cadmium 50 mM 48 hrs, 4-day adult    362 Cadmium 100 mM 48 hrs, 4-day adult    306 Copper 0.5 mM 12 hrs, larvae L3    135 Copper 15 mM 48 hrs, 4-day adult    74 Zinc 5 mM 12 hrs, larvae L3    136 Zinc 4.5 mM 48 hrs, 4-day adult    56 Ethanol 2.5% 3 hrs, larvae L3    295 Ethanol 5% 3 hrs, larvae L3    627 Ethanol 10% 3 hrs, larvae L3    512 Caffeine 1.5 mg/ml 4 hrs, larvae L3    811 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    235 Caffeine 25 mg/ml 48 hrs, 4-day adult    90 Paraquat 5 mM 48 hrs, 4-day adult    105 Paraquat 10 mM 48 hrs, 4-day adult    454 Rotenone 2 μg 12 hrs, larvae L3    458 Rotenone 8 μg 12 hrs, larvae L3    792 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (88) cold shock, 4-day adult  (54) heat shock, 4-day adult  (321) Cadmium 50 mM 6 hrs, larvae L3  (608) Cadmium 50 mM 12 hrs, larvae L3  (289) Cadmium 50 mM 48 hrs, 4-day adult  (362) Cadmium 100 mM 48 hrs, 4-day adult  (306) Copper 0.5 mM 12 hrs, larvae L3  (135) Copper 15 mM 48 hrs, 4-day adult  (74) Zinc 5 mM 12 hrs, larvae L3  (136) Zinc 4.5 mM 48 hrs, 4-day adult  (56) Ethanol 2.5% 3 hrs, larvae L3  (295) Ethanol 5% 3 hrs, larvae L3  (627) Ethanol 10% 3 hrs, larvae L3  (512) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (811) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (235) Caffeine 25 mg/ml 48 hrs, 4-day adult  (90) Paraquat 5 mM 48 hrs, 4-day adult  (105) Paraquat 10 mM 48 hrs, 4-day adult  (454) Rotenone 2 μg 12 hrs, larvae L3  (458) Rotenone 8 μg 12 hrs, larvae L3  (792) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    88 cold shock, 4-day adult    54 heat shock, 4-day adult  (321) Cadmium 50 mM 6 hrs, larvae L3  (608) Cadmium 50 mM 12 hrs, larvae L3  (289) Cadmium 50 mM 48 hrs, 4-day adult  (362) Cadmium 100 mM 48 hrs, 4-day adult  (306) Copper 0.5 mM 12 hrs, larvae L3  (135) Copper 15 mM 48 hrs, 4-day adult    74 Zinc 5 mM 12 hrs, larvae L3  (136) Zinc 4.5 mM 48 hrs, 4-day adult    56 Ethanol 2.5% 3 hrs, larvae L3  (295) Ethanol 5% 3 hrs, larvae L3  (627) Ethanol 10% 3 hrs, larvae L3  (512) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (811) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (235) Caffeine 25 mg/ml 48 hrs, 4-day adult    90 Paraquat 5 mM 48 hrs, 4-day adult  105 Paraquat 10 mM 48 hrs, 4-day adult  (454) Rotenone 2 μg 12 hrs, larvae L3  (458) Rotenone 8 μg 12 hrs, larvae L3  (792) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    88 cold shock, 4-day adult    54 heat shock, 4-day adult    321 Cadmium 50 mM 6 hrs, larvae L3    608 Cadmium 50 mM 12 hrs, larvae L3    289 Cadmium 50 mM 48 hrs, 4-day adult    362 Cadmium 100 mM 48 hrs, 4-day adult    306 Copper 0.5 mM 12 hrs, larvae L3    135 Copper 15 mM 48 hrs, 4-day adult    74 Zinc 5 mM 12 hrs, larvae L3    136 Zinc 4.5 mM 48 hrs, 4-day adult    56 Ethanol 2.5% 3 hrs, larvae L3    295 Ethanol 5% 3 hrs, larvae L3    627 Ethanol 10% 3 hrs, larvae L3    512 Caffeine 1.5 mg/ml 4 hrs, larvae L3    811 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    235 Caffeine 25 mg/ml 48 hrs, 4-day adult    90 Paraquat 5 mM 48 hrs, 4-day adult    105 Paraquat 10 mM 48 hrs, 4-day adult    454 Rotenone 2 μg 12 hrs, larvae L3    458 Rotenone 8 μg 12 hrs, larvae L3    792 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    88 cold shock, 4-day adult    54 heat shock, 4-day adult    321 Cadmium 50 mM 6 hrs, larvae L3    608 Cadmium 50 mM 12 hrs, larvae L3    289 Cadmium 50 mM 48 hrs, 4-day adult    362 Cadmium 100 mM 48 hrs, 4-day adult    306 Copper 0.5 mM 12 hrs, larvae L3    135 Copper 15 mM 48 hrs, 4-day adult    74 Zinc 5 mM 12 hrs, larvae L3    136 Zinc 4.5 mM 48 hrs, 4-day adult    56 Ethanol 2.5% 3 hrs, larvae L3    295 Ethanol 5% 3 hrs, larvae L3    627 Ethanol 10% 3 hrs, larvae L3    512 Caffeine 1.5 mg/ml 4 hrs, larvae L3    811 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    235 Caffeine 25 mg/ml 48 hrs, 4-day adult    90 Paraquat 5 mM 48 hrs, 4-day adult    105 Paraquat 10 mM 48 hrs, 4-day adult    454 Rotenone 2 μg 12 hrs, larvae L3    458 Rotenone 8 μg 12 hrs, larvae L3    792 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (88) cold shock, 4-day adult  (54) heat shock, 4-day adult  (321) Cadmium 50 mM 6 hrs, larvae L3  (608) Cadmium 50 mM 12 hrs, larvae L3  (289) Cadmium 50 mM 48 hrs, 4-day adult  (362) Cadmium 100 mM 48 hrs, 4-day adult  (306) Copper 0.5 mM 12 hrs, larvae L3  (135) Copper 15 mM 48 hrs, 4-day adult  (74) Zinc 5 mM 12 hrs, larvae L3  (136) Zinc 4.5 mM 48 hrs, 4-day adult  (56) Ethanol 2.5% 3 hrs, larvae L3  (295) Ethanol 5% 3 hrs, larvae L3  (627) Ethanol 10% 3 hrs, larvae L3  (512) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (811) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (235) Caffeine 25 mg/ml 48 hrs, 4-day adult  (90) Paraquat 5 mM 48 hrs, 4-day adult  (105) Paraquat 10 mM 48 hrs, 4-day adult  (454) Rotenone 2 μg 12 hrs, larvae L3  (458) Rotenone 8 μg 12 hrs, larvae L3  (792) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    88 cold shock, 4-day adult    54 heat shock, 4-day adult  (321) Cadmium 50 mM 6 hrs, larvae L3  (608) Cadmium 50 mM 12 hrs, larvae L3  (289) Cadmium 50 mM 48 hrs, 4-day adult  (362) Cadmium 100 mM 48 hrs, 4-day adult  (306) Copper 0.5 mM 12 hrs, larvae L3  135 Copper 15 mM 48 hrs, 4-day adult    74 Zinc 5 mM 12 hrs, larvae L3  136 Zinc 4.5 mM 48 hrs, 4-day adult    56 Ethanol 2.5% 3 hrs, larvae L3  (295) Ethanol 5% 3 hrs, larvae L3  (627) Ethanol 10% 3 hrs, larvae L3  (512) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (811) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (235) Caffeine 25 mg/ml 48 hrs, 4-day adult    90 Paraquat 5 mM 48 hrs, 4-day adult  105 Paraquat 10 mM 48 hrs, 4-day adult  (454) Rotenone 2 μg 12 hrs, larvae L3  (458) Rotenone 8 μg 12 hrs, larvae L3  (792) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    88 cold shock, 4-day adult    54 heat shock, 4-day adult    321 Cadmium 50 mM 6 hrs, larvae L3    608 Cadmium 50 mM 12 hrs, larvae L3    289 Cadmium 50 mM 48 hrs, 4-day adult    362 Cadmium 100 mM 48 hrs, 4-day adult    306 Copper 0.5 mM 12 hrs, larvae L3    135 Copper 15 mM 48 hrs, 4-day adult    74 Zinc 5 mM 12 hrs, larvae L3    136 Zinc 4.5 mM 48 hrs, 4-day adult    56 Ethanol 2.5% 3 hrs, larvae L3    295 Ethanol 5% 3 hrs, larvae L3    627 Ethanol 10% 3 hrs, larvae L3    512 Caffeine 1.5 mg/ml 4 hrs, larvae L3    811 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    235 Caffeine 25 mg/ml 48 hrs, 4-day adult    90 Paraquat 5 mM 48 hrs, 4-day adult    105 Paraquat 10 mM 48 hrs, 4-day adult    454 Rotenone 2 μg 12 hrs, larvae L3    458 Rotenone 8 μg 12 hrs, larvae L3    792 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_10 mE1_20_mRNA_expression_cluster_10
External Data & Images
Linkouts	BDGP expression data • RH47312 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001149 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10045-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for GstD1 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 GstD1GD6846 Phenotype manifest in Allele
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of GstD1 Class Mutagen Stocks Known lesion GstD1d07341 1 -- GstD1G15553 P-element activity 1 -- GstD133f1 0 Yes GstD1d11179 0 --
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of GstD1 Class Mutagen Stocks Known lesion GstD1GD6846 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes GstD1GL01039 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes GstD1KK112645 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes GstD1hs.PA in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes GstD1hs.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes GstD1NIG.10045R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes GstD1Scer\UAS.cPa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)Δ33-4 (Pielage et al., 2004) Df(3R)Exel6164 (Ryder, 2004.4.26) Df(3R)Exel6165 (Ryder, 2004.4.26) Df(3R)Exel7314 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data heat-shock construct P{hs-GstD1.D1F-UTR} NA P{hs-GstD1.D1-UTR} NA reporter construct P{GstD1-lacZ.S} No UAS construct P{GD6846} NA P{KK112645} NA P{NIG.10045R} NA P{TRiP.GL01039} NA characterization construct P{hs-GstD1::GstD2.D1-66-D21} NA P{hs-GstD1::GstD2.D1-171-D21} NA P{hs-GstD1::GstD2.D21-65-D1} NA P{hs-GstD1::GstD2.D21-170-D1} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}GstD1G15553 NA P{EPgy2}EY02348 NA P{XP}GstD1d04829 NA P{XP}GstD1d07341 NA P{XP}GstD1d11179 NA miscellaneous insertions P{UAST-YFP.Rab18.S19N}03 NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function
CV term Evidence References
DDT-dehydrochlorinase activity inferred from direct assay (Low et al., 2010) glutathione transferase activity inferred from direct assay (Cochrane and Leblanc, 1986, Sawicki et al., 2003, Saisawang et al., 2012)
Biological Process
CV term Evidence References
glutathione metabolic process inferred from direct assay (Saisawang et al., 2012)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
glutathione transferase activity inferred from sequence or structural similarity with UniProtKB:P42860 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1991.2.1)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
cytoplasm inferred from sequence or structural similarity with rat Gsto1 (Curators, 2008--)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
GstD1 - Arpc1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstD1 - CG10581 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstD1 - CG6321 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstD1 - Esp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstD1 - GstD10 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) GstD1 - GstD2 experimental knowledge based (Guruharsha et al., 2011) GstD1 - GstD4 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) GstD1 - GstD5 experimental knowledge based (Guruharsha et al., 2011) GstD1 - GstD7 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) GstD1 - GstD9 experimental knowledge based (Guruharsha et al., 2011) GstD1 - Oli anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) GstD1 - mRpL52 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions GstD1 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66612 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0001149 DroID - A comprehensive database of gene and protein interactions. • FBgn0001149 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41503
Orthologs
OrthoDB Orthologs (77) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6BP1Z4 Dipteran inclusive ortholog search EOG6SBF6X Insect inclusive ortholog search EOG6X96DJ Arthropod inclusive ortholog search EOG6QFTWF Metazoa inclusive ortholog search EOG6KHFPN
Orthologs in Drosophila Species (EOG6BP1Z4)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\GstD1     Drosophila simulans   Dsim\GstD1 Y   Drosophila sechellia   Dsec\GstD1 Y   Drosophila erecta   Dere\GstD1 Y   Drosophila yakuba   Dyak\GstD1 Y   Drosophila ananassae   Dana\GF17052 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA10031 Y   Drosophila persimilis   Dper\GL27184 Y   Drosophila willistoni   Dwil\GK11204 Y   Drosophila virilis   Dvir\GJ24385 Y   Drosophila mojavensis   Dmoj\GI24379     Drosophila mojavensis   Dmoj\GI22354     Drosophila grimshawi   Dgri\GH20186     Drosophila grimshawi   Dgri\GH13103
Orthologs in non-Drosophila Dipterans (EOG6SBF6X)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL001078 Y Aedes aegypti Yellow fever mosquito  Aaeg\AAEL001061 Y Anopheles gambiae Malaria mosquito  Agam\GstD1 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ002661 Y
Orthologs in non-Dipteran Insects (EOG6X96DJ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB50265 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG012852 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG005033 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG005031 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG001186 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH11989 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH11987 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH11990 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH11991 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH11986 Y Atta cephalotes Leafcutter ant  Acep\ACEP23697 Y Atta cephalotes Leafcutter ant  Acep\ACEP15391 Y Atta cephalotes Leafcutter ant  Acep\ACEP23691 Y Atta cephalotes Leafcutter ant  Acep\ACEP12205 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO18580 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO18579 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO18581 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL17884 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL17885 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL17883 Y Linepithema humile Argentine ant  Lhum\LH25411 Y Linepithema humile Argentine ant  Lhum\LH12900 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB24593 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB24592 Y Solenopsis invicta Red fire ant  Sinv\SINV16014 Y Solenopsis invicta Red fire ant  Sinv\SINV15995 Y Solenopsis invicta Red fire ant  Sinv\SINV15961 Y Solenopsis invicta Red fire ant  Sinv\SINV15978 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI006598 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI008550 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI001068 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI006899 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI008657 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI005620 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI085935 Y Bombyx mori Silkmoth  Bmor\GST delta Y Bombyx mori Silkmoth  Bmor\BGIBMGA002211 Y Bombyx mori Silkmoth  Bmor\BGIBMGA006537 Y Bombyx mori Silkmoth  Bmor\BGIBMGA006538 Y Pediculus humanus Human body louse  Phum\PHUM189430 Y Pediculus humanus Human body louse  Phum\PHUM097960 Y Pediculus humanus Human body louse  Phum\PHUM189440 Y Tribolium castaneum Red flour beetle  Tcas\TC009482 Y Tribolium castaneum Red flour beetle  Tcas\GLEAN_07571 Y Tribolium castaneum Red flour beetle  Tcas\TC004450 Y
Orthologs in non-Insect Arthropods (EOG6QFTWF)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\azu Y Daphnia pulex Water flea  Dpux\JGI_V11_196080 Y Daphnia pulex Water flea  Dpux\JGI_V11_304194 Y Daphnia pulex Water flea  Dpux\azu Y Daphnia pulex Water flea  Dpux\JGI_V11_230826 Y Ixodes scapularis Deer tick  Isca\ISCW024825 Y Ixodes scapularis Deer tick  Isca\ISCW003686 Y Ixodes scapularis Deer tick  Isca\ISCW024802 Y Ixodes scapularis Deer tick  Isca\ISCW024667 Y Ixodes scapularis Deer tick  Isca\ISCW005803 Y Ixodes scapularis Deer tick  Isca\ISCW011508 Y
Orthologs in non-Arthropod Metazoa (EOG6KHFPN)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006495gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009239gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_021662gn Y
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (9) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GstD1 Drosophila sechellia Dsec\GstD1 Drosophila erecta Dere\GstD1 Drosophila yakuba Dyak\GstD1 Drosophila ananassae Dana\GF17052 Drosophila pseudoobscura pseudoobscura Dpse\GA10031 Drosophila persimilis Dper\GL27184 Drosophila willistoni Dwil\GK11204 Drosophila virilis Dvir\GJ24385
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	26926 y1 w*; P{EP}GstD1G15553 36818 y1 sc* v1; P{TRiP.GL01039}attP2/TM3, Sb1
Harvard	- P{XP}GstD1d07341
VDRC	v103246 P{KK112645}VIE-260B v17969 w1118 P{GD6846}v17969
Genomic Clones ( 2 )
	BACR28I14 BACR44K17 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 199 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP01777 RH47312
Other clones	DPiM_RH47312 FI16812 IP20147 yak-em_GstD1
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH03448 GH03460 GH07673 GH07784 GH11034 GH16867 GH22301 GH24223 GH24307 GH24442 GH25057 GH25753 GM02020 GM09644 GM09926 GM13444 GM19874 GM22128 GM26288 GM29237 GM32367 LD14723 LP06018 LP06820 LP11533 LP11554 LP12105 LP12138 LP12319 LP15350 LP19951 RE06274 RE07083 RE16346 RE16570 RE19066 RE33216 RE34673 RE44009 RE56727 RE65110 RE65215 RE67818 RE67938 RE69010 RE72944 RH04551 RH05509 RH05968 RH05994 RH08336 RH08355 RH12264 RH22717 RH22974 RH24289 RH26952 RH34382 RH34512 RH34914 RH36566 RH38826 RH40535 RH41868 RH56132 RH58532 RH61202 RH62829 RH63329 RH66495 RH67765 RH70122
Other clones	79679 106888 117758 145599 153491 523409 524044 524767 524916 533973 561884 562200 568889 572729 576495 bs11d05 bs33e08 bs44c11 bs71h01 bs81a08 CK01.118C.G10 CK01.126D.H6 CK01.127C.H6 CK01.129D.H2 CK01.145D.H3 Dmel_dig_000833_0594_2367 Dmel_dig_000838_0631_0292 Dmel_dig_001800_0647_3712 Dmel_dig_004956_0642_1708 Dmel_dig_005799_0706_1649 Dmel_dig_006397_0698_2195 Dmel_dig_006734_0639_0674 Dmel_dig_007488_0643_1673 Dmel_dig_007736_0671_0971 Dmel_dig_010525_0674_1440 Dmel_dig_011013_0686_2442 Dmel_dig_012577_0685_1038 Dmel_dig_013812_0662_3126 Dmel_dig_014315_0717_1215 Dmel_dig_018677_0674_1313 Dmel_dig_019925_0615_3220 Dmel_neb_006600_0628_3613 Dmel_neb_008477_0944_3003 Dmel_neb_009545_0693_2623 Dmel_neb_010581_0884_3527 Dmel_neb_011434_0927_2775 Dmel_neb_014551_0825_0645 Dmel_neb_016310_0833_2051 EC13521 EC22121 EC28193 EC28773 EC29293 EC32717 EC34080 EC40369 EK089957 EK097163 EK097748 EK119830 EK237744 EK256842 EN03851 EN04647 EN05863 EN06152 EN09944 EN13321 EN13912 EN14128 EN16936 EN17761 ESG0114b.B21_F10 ESG0114d.B21_A06 FGC006F02 FGC028A11 FGH110F09 FGM223D02 FGM231D09 FGM307H05 FGM322A01 RH09431 RP003051279 RP003057040 RP003075953 RP003090899 RP003126265 RP003139979 RP003144223 RP003145212 RP003243932 RP003245705 RP003281703 RP003288231 RP003288878 RP003292939 RP003306150 RP003307054 RP003320863 RP003322197 RP003343506 RP003417068 RP003422837 RP003431264 RP003473151 RP003483460 RP003497114 RP003500011 RP003500708 RP003505902 RP003517307 RP003518725 RP003535494 RP003577525 RP003611431 RP003634776 RP003657180 RP003659616 RP003661474 RP003669852 UUGC0513
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0001149 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41503
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Quantifying rates of protein sequence divergence within and between species reveals that the Drosophila genome harbors a substantial proportion of genes with a very high divergence rate. (Schmid and Tautz, 1997) mRNA levels for GST do not increase at adult day 5 in strain showing extended longevity phenotype (ELP). (Dudas and Arking, 1995) Glutathione S transferase activity has been analysed in various developmental stages. (Hunaiti et al., 1995) Pentobarbital rapidly induces the expression of GstD1, by activating transcription. (Tang and Tu, 1995) Dehydro-chlorinase activity of the protein is determined by biochemical analysis using gas-liquid chromatography to assess the contribution of the isozymes to the DDT-resistant phenotype. (Tang and Tu, 1994) Encodes glutathione S transferase subunit 1 (Toung et al., Biochem. biophys. Res. Commun. 178: 1205--1211, Toung and Tu, Biochem. biophys. Res. Commun. 182: 355--360). (Toung et al., 1993) GstD1 locus is flanked by two clusters of heat shock genes. Enzyme activity in Kco cells is elevated slightly to two fold under heat shock. (Toung et al., 1991) The GstD1 gene function has been implicated in repairing cellular damage inflicted by oxygen free radicals. (Lloyd et al., 1990) Glutathione S-transferase enzyme has been purified and characterised. (Cochrane et al., 1987) Glutathione S-transferase activity shows differences in age dependence, substrate selectivity and subcellular location in adult Drosophila, suggesting the presence of multiple forms of the enzyme. Differences in activity between different Drosophila strains are also seen. (Ottea et al., 1987) Substrate specificities and pH optima suggest that GstD1 enzyme has a restricted range of substrates. Pentamethyl benzene (PMB) causes a 50% increase in enzyme activity, whereas β,naphthoflavone and phenobarbital have no effect. The degree of response to PMB is modulated by other genes on chromosome 2. Response of enzyme activity to 1-chloro-2,3-dinitrobenzene (CDNB) and ethacrynic acid (EA) can also differ. (Cochrane and Leblanc, 1986) GstD1 gene product does not conjugate the epoxides styrene-7,8-oxide and 1,2-epoxy-3-propane. (Jansen et al., 1984) Glutathione S-transferase activity has been investigated in sub-cellular fractions of D.melanogaster. (Baars et al., 1979)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079602 NM_001038953 RefSeq (Proteins) • NP_524326 NP_001034042 DDBJ / EMBL / GenBank • AA803543 AAA04220 AAF54786 AAF54786.1 AAM52032 ABC66168 AI108593 AI945315 AI946965 AY121705 BI617087 CAA32449 M97702 S51044 X14233 Entrez Gene - A searchable database of RefSeq genes. • 41503 UniProtKB/Swiss-Prot • P20432
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Glutathione S-transferase, N-terminal (IPR004045) Glutathione S-transferase, C-terminal (IPR004046) Glutathione S-transferase, C-terminal-like (IPR010987) Thioredoxin-like fold (IPR012336) Glutathione S-transferase/chloride channel, C-terminal (IPR017933) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RH47312
Linkouts
	BioGRID - A database of protein and genetic interactions • 66612 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0001149 DroID - A comprehensive database of gene and protein interactions. • FBgn0001149 DRSC - Results from RNAi screens. • FBgn0001149 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001149 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10045-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0001149 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41503 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41503 modMine - Data generated by the modENCODE project. • FBgn0001149
Synonyms & Secondary IDs ( 37 )
Reported As
Symbol Synonym	CG10045 (Vierstraete et al., 2004, Sun et al., 2010, Thornton et al., 2010, Ni et al., 2011.7.19, Keleman et al., 2009.8.5, Marco et al., 2004, Ling and Salvaterra, 2011, Alias and Clark, 2007, Cammarato et al., 2011) D1 (Alias and Clark, 2007) DmGST1 (Ranson et al., 2001, Lougarre et al., 1999, Toung et al., 1993, Morton, 1993, Murray et al., 1993) DmGST-1 (Chelvanayagam et al., 2001, Beall et al., 1992) DmGst1 (Toung et al., 1991) DmGSTD1 (Sawicki et al., 2003, Chelvanayagam et al., 2001, Zimniak and Benes, 2002.7.15, Low et al., 2010) dmGSTD1 (Low et al., 2010) DmGSTD1-1 (Sharma et al., 2004, Singh et al., 2002, Sawicki et al., 2002) Gst (Ranz et al., 2001, Ranz et al., 2001, Lloyd et al., 1990, Faridwajidi, 1993, Faridwajidi and Jowett, 1993, ) GST (Arking, 1998, Arking et al., 1996, Harshman et al., 1991, Ottea et al., 1987) G-S-T (Tautz and Schmid, 1998) GST-1 (Pielage et al., 2004, Agianian et al., 2003, Singh et al., 2002, Clayton et al., 1998) GST1 (Le Goff et al., 2001) Gst1 (Toung et al., 1991) Gst1-1 (Schmid and Tautz, 1997) GST1-1 (Pearson, 1996, Toung et al., 1990) GSt1-1 (Akaboshi et al., 1994) Gst1.1   GSTase (Cochrane et al., 1987) gst-D (Milton et al., 2011) gstD (Tuxworth et al., 2011) GSTD1 (Wang et al., 2003, Zou et al., 2000, Low et al., 2008, Tohyama and Yamaguchi, 2010, Low et al., 2007, Hooven et al., 2009, Li et al., 2008, Low et al., 2010, Alias and Clark, 2007) gstD1 (Akgul and Tu, 2002, Tang and Tu, 1995, Tang and Tu, 1994, Toung et al., 1993, Karpac et al., 2009, Grimberg et al., 2011, Hochmuth et al., 2011, Sykiotis and Bohmann, 2008, Sharma et al., 2011) GstD1 (Sun et al., 2010, Zhang et al., 2007, Chahine and O'Donnell, 2011, Allen and Spradling, 2008, Gilchrist et al., 2008, Morozova et al., 2006, Anholt and Williams, 2010, Ling and Salvaterra, 2011, Boyd et al., 2011, Japanese National Institute of Genetics, 2012.5.21, Awofala et al., 2012) gstd1 (Wayne et al., 2011, Saisawang et al., 2012) GST-D1 (Wagner et al., 2009) Gst-D1 (Nakamura et al., 1999) gst-D1-1 (Singh et al., 2000) T5 (Vierstraete et al., 2004) unnamed (Hunaiti et al., 1995)
Name Synonym	CG10045 Glutathione S-transferase (Kang et al., 2002) Glutathione S-transferase 1 (Pearson, 1998) Glutathione-S-transferase 1 (Ling and Salvaterra, 2011) Glutathione S transferase D1 (Varma and Mishra, 2011, Cammarato et al., 2011) glutathione S-transferase D1 (Rand et al., 2009, Boyd et al., 2011) Glutathione S-transferase-D1 (Nakamura et al., 1999) Glutathione S-transferase D1 (Sun et al., 2007)
Secondary FlyBase IDs
	FBgn0010045
References ( 123 )
Generate a list of	All references relating to this gene ( 123 )
List References by type	Research paper  ( 82 ) Supplementary material  ( 8 ) Review  ( 4 ) Personal communication to FlyBase  ( 11 ) Abstract  ( 9 ) FlyBase analysis  ( 3 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 16 )
	Awofala et al., 2012, Funct. Integr. Genomics 12(2): 305--315 Functional roles for redox genes in ethanol sensitivity in Drosophila. [FBrf0218408] Beaver et al., 2012, PLoS ONE 7(11): e50454 Circadian Regulation of Glutathione Levels and Biosynthesis in Drosophila melanogaster. [FBrf0220135] Landis et al., 2012, Aging 4(11): 768--789 Gene expression changes in response to aging compared to heat stress, oxidative stress and ionizing radiation in Drosophila melanogaster. [FBrf0220226] Saisawang et al., 2012, Biochem. J. 442(1): 181--190 A preliminary characterization of the cytosolic glutathione transferase proteome from Drosophila melanogaster. [FBrf0217315] Wongsantichon et al., 2012, Arch. Biochem. Biophys. 521(1-2): 77--83 Structural evidence for conformational changes of Delta class glutathione transferases after ligand binding. [FBrf0218684] Wongtrakul et al., 2012, J. Insect Sci. 12: 107 Mitogen-Activated Protein Kinase p38b Interaction with Delta Class Glutathione Transferases from the Fruit Fly, Drosophila melanogaster. [FBrf0220909] Boyd et al., 2011, Free Radical Biol. Med. 50(11): 1669--1678 Nectarine promotes longevity in Drosophila melanogaster. [FBrf0213612] Chahine and O'Donnell, 2011, J. Exp. Biol. 214(3): 462--468 Interactions between detoxification mechanisms and excretion in Malpighian tubules of Drosophila melanogaster. [FBrf0212764] Grimberg et al., 2011, Mol. Cell. Biol. 31(4): 897--909 Basic Leucine Zipper Protein Cnc-C Is a Substrate and Transcriptional Regulator of the Drosophila 26S Proteasome. [FBrf0212906] Hochmuth et al., 2011, Cell Stem Cell 8(2): 188--199 Redox regulation by keap1 and nrf2 controls intestinal stem cell proliferation in Drosophila. [FBrf0212945] Ling and Salvaterra, 2011, PLoS ONE 6(3): e17762 Robust RT-qPCR Data Normalization: Validation and Selection of Internal Reference Genes during Post-Experimental Data Analysis. [FBrf0213272] Milton et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(42): 17521--17526 Oxidative stress induces overgrowth of the Drosophila neuromuscular junction. [FBrf0216440] Sharma et al., 2011, Chemosphere 82(3): 370--376 Transcriptome analysis provides insights for understanding the adverse effects of endosulfan in Drosophila melanogaster. [FBrf0212610] Tuxworth et al., 2011, Hum. Mol. Genet. 20(10): 2037--2047 The Batten disease gene CLN3 is required for the response to oxidative stress. [FBrf0213533] Varma and Mishra, 2011, J. Biosci., Bangalore 36(3): 439--459 Dynamics of nuclear matrix proteome during embryonic development in Drosophila melanogaster. [FBrf0214552] Wayne et al., 2011, Evolution 65(4): 1068--1078 EXPRESSION OF DEFENSE GENES IN DROSOPHILA EVOLVES UNDER A DIFFERENT SELECTIVE REGIME FROM EXPRESSION OF OTHER GENES. [FBrf0213376] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...