ERCC3, XPB, TFIIH, DmXPB, nc2
Gene model reviewed during 5.43
Shares 5' UTR with downstream gene.
Gene model reviewed during 5.46
Component of the 7-subunit TFIIH core complex composed of haywire/XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription. Interacts with PUF60. Interacts with ATF7IP. Interacts with Epstein-Barr virus EBNA2.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\hay using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\hay in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: hay CG8019
Mutant lesions in lethal hay alleles reveal the importance of the nucleotide-binding domain, suggesting an essential role for ATPase activity.
Gene is involved in pre-replication DNA repair of UV and AA lesions.
mRNA levels do not increase at adult day 5 in strain showing extended longevity phenotype (ELP).
Low-stringency hybridization isolated the homolog of the human excision repair gene ERCC3, which maps to 67E3--67E4, known to be the previously defined haywire gene. Microinjection and DNA transfection of haywire into human xeroderma pigmentosum fibroblasts and group 3 rodent mutants did not yield detectable correction.
The hay gene was cloned and is related to the human ERCC3 gene which is associated with both xeroderma pigmentosum and Cockayne's syndrome. Phenotypes of hay mutants mimic some of the effects of xeroderma pigmentosum in humans.
The original allele, haync2, is homozygous male sterile; some derivative alleles are recessive lethal. Homozygous haync2 males display defects in meiosis, flagellar elongation and nuclear shaping. haync2 also male sterile in transheterozygotes with recessive male-sterile alleles of β Tub85D or with wrl1 suggesting a role for the haywire product in microtubule function. Deficiencies of hay are fertile in heterozygous combination with the above interacting mutations, indicating that the extragenic failure haync2 to complement is based on a poison product mechanism. This feature was used to select alleles (haync2rv1-8) that revert the failure of haync2 to complement βTub85Dn.