A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Gene Dmel\His1

General Information
SymbolDmel\His1SpeciesD. melanogaster
NameHistone H1Annotation symbol
Feature typeprotein_coding_geneFlyBase IDFBgn0001195
Gene Model StatusNot Applicable Stock availability None publicly available
Also Known AsH1, HisC
Genomic Location
Chromosome (arm)Recombination map2-55
Cytogenetic map39D3-39E1Sequence location
hide Summary Information
Automatically generated summary

See sections below for more information
The gene Histone H1 is referred to in FlyBase by the symbol Dmel\His1 (FBgn0001195). This gene record represents a gene family, individual members of the family are: CG31617, CG33801, CG33804, CG33807, CG33810, CG33813, CG33816, CG33819, CG33822, CG33825, CG33828, CG33831, CG33834, CG33837, CG33840, CG33843, CG33846, CG33849, CG33852, CG33855, CG33858, CG33861, CG33864. It is reported to have molecular function: DNA binding. There is experimental evidence that it is involved in the biological process: negative regulation of histone modification. 4 alleles are reported. The phenotypes of these alleles are annotated with: polytene chromosome; polytene chromosome chromocenter. It has no annotated transcripts. Protein features are: Histone H1/H5; Histone H5; Winged helix-turn-helix transcription repressor DNA-binding. Gene has not been localized to the genome sequence.
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Description
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FB2011_10
FB2012_01
References
Ohsawa et al., 2008, Cell Death Differ. 15(9): 1429--1439
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
39D3-39E1  
Left limit from in situ hybridisation (FBrf0029738) Right limit from molecular mapping relative to His2A (FBrf0044950)  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
39D-39E  
(determined by in situ hybridisation)  
(Fitch et al., 1990)
39D3-39E2  
(determined by in situ hybridisation)  
(Pardue et al., 1977)
Experimentally Determined Recombination Data
Location
2-55
 
Left of (cM)
Right of (cM)
Notes
hide Gene Model & Products
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Histone H1/H5 (IPR005818)
Histone H5 (IPR005819)
Winged helix-turn-helix transcription repressor DNA-binding (IPR011991)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
BT021375
AAX33523
 
X04073
CAA27716
 
X14215
CAA32433
 
X60225
CAA42786
 
Z31936
 
Z70810
 
Z71060
 
Z71061
 
Z83369
 
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
hide Mapped Features
Type
Symbol & Location
Additional Notes
References
hide External Data
Linkouts
Crossreferences
EPD - Eukarytoic Promoter Database, an annotated collection of POL II promoters
•
hide Expression Data
hideTranscript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
hidePolypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 5  
cell  
• condensed nuclear chromosome
(Ji et al., 2002)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
hide High-Throughput Expression Data
hide Expression Clusters
hide External Data & Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
•
hide Alleles & Phenotypes
hide Summary of Allele Phenotypes
Lethality
Allele
lethal, with Scer\GAL4da.G32
Phenotype manifest in
Allele
polytene chromosome, with Scer\GAL4da.G32
polytene chromosome chromocenter, with Scer\GAL4da.G32
hide Classical Alleles ( 0 )
For All Classical Alleles Show

Allele of His1ClassMutagenStocksKnown lesion
hide Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show

Allele of His1ClassMutagenStocksKnown lesion
His1dsRNA.Scer\UAS.cSa0 Yes
His1MNPV(Op)\IE-2.T:Avic\GFP,T:SV5\V5,T:Zzzz\His60 Yes
His1Scer\UAS.T:Avic\GFP-EYFP0 Yes
His1tub.T:Zzzz\FLAG.T:Avic\GFP-CFP0 Yes
hide Aneuploid Aberrations
Disrupted in
(Moore et al., 1981)
Duplicated in
(Moore et al., 1981)
hide Transgenic Constructs & Insertions
Transgenic Constructs
Type of construct
Name
Expression data
UAS construct
P{UAS-His1.dsRNA}
NA
P{UAS-His1.EYFP}
NA
characterization construct
P{tub-His1.flag-CFP}
NA
Insertions
Type of insertions
Name
Expression data
hide Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
hide Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term
Evidence
References
negative regulation of histone modification
inferred from direct assay
(Ni et al., 2006)
Cellular Component ( 0 terms)
hide Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term
Evidence
References
DNA binding
inferred from electronic annotation with InterPro:IPR005818, InterPro:IPR005819
(FlyBase Curators et al., 2004-)
non-traceable author statement
(Swiss-Prot Project Members, 1986.7.21)
Biological Process
CV term
Evidence
References
chromatin assembly or disassembly
non-traceable author statement
(Swiss-Prot Project Members, 1986.7.21)
nucleosome assembly
inferred from electronic annotation with InterPro:IPR005818, InterPro:IPR005819
(FlyBase Curators et al., 2004-)
Cellular Component
CV term
Evidence
References
nucleosome
inferred from electronic annotation with InterPro:IPR005818, InterPro:IPR005819
(FlyBase Curators et al., 2004-)
nucleus
inferred from electronic annotation with InterPro:IPR005818
(FlyBase Curators et al., 2004-)
non-traceable author statement
(Swiss-Prot Project Members, 1986.7.21)
hide Sequence Ontology: Class of Gene
member_gene_array
 
nuclear_gene
 
protein_coding_gene
 
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hide Summary of Physical Interactions
Protein-protein
Interacting group
Assay
References
hide Summary of Genetic Interactions
Interacts with
Please look at the allele data for full details of the genetic interactions
His1 allele
Gene
References
hide External Data
Linkouts
hide Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs
 
 
 
 
 
 
 
 
 
 
 
 
Linkouts
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hide Stocks Listed in FlyBase ( 0 )
hide Genomic Clones ( 0 )
hide cDNA Clones ( 0 )
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
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Linkouts
hide Antibody Information
polyclonal
(Braunschweig et al., 2009)
hide Other Information
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hide Etymology
hide Identification
hide Relationship to Other Genes
Source for database identity of
Source for database merge of
Encoded by
HIS-C
 
Component gene(s)
His1:CG31617
 
His1:CG33801
 
His1:CG33804
 
His1:CG33807
 
His1:CG33810
 
His1:CG33813
 
His1:CG33816
 
His1:CG33819
 
His1:CG33822
 
His1:CG33825
 
His1:CG33828
 
His1:CG33831
 
His1:CG33834
 
His1:CG33837
 
His1:CG33840
 
His1:CG33843
 
His1:CG33846
 
His1:CG33849
 
His1:CG33852
 
His1:CG33855
 
His1:CG33858
 
His1:CG33861
 
His1:CG33864
 
Additional comments
hide Other Comments
DNA-protein interactions: genome-wide binding profile assayed for His1 protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069).
(Filion et al., 2010)
dsRNA made from templates generated with primers directed against this gene was transfected onto wild-type Kc cells to determine whether the presence of His1 is essential for the association of ribosomal proteins on chromatin. Using this method, His1 is considered essential for the association of ribosomal proteins with chromatin.
(Ni et al., 2006)
His1 is misexpressed in the PNS of da mutant embryos.
(Hassan and Vaessin, 1997)
Chromatin in a cell free chromatin assembly system derived from embryos contains an activity that hydrolyses ATP to render entire nucleosome arrays mobile even in the absence of ATP, and even in the presence of histone H1.
(Varga-Weisz et al., 1995)
Efficient repression of transcription by polymerase II in this system does not require the presence of His1.
(Sandaltzopoulos et al., 1994)
The distribution of His1 protein on the 'Alu-repeat' DNA in the non-transcribed spacer of the ribosomal repeat (bb) and on 1.688-g/cm3 satDNA has been mapped.
(Belikov et al., 1993)
polo gene product immunoprecipitated from single Drosophila embryos can phosphorylate casein in vitro, and the kinase activity peaks cyclically at late anaphase/telophase. This contrasts with the cycling of CycB associated p34cdc2 histone H1 kinase, which is maximal upon entry into mitosis during the rapid syncitial mitoses.
(Fenton and Glover, 1993)
The codon bias of the histone genes from D.melanogaster and D.hydei illustrates that the generalisation that abundantly expressed genes have a high codon bias and low rates of silent substitution does not hold for the histone genes.
(Fitch and Strausbaugh, 1993)
The position of the homologous histone gene repeats within the nuclei of early embryo cells has been investigated. The two homologous histone gene clusters are distinct and separate through all stages of the cell cycle up to nuclear cycle 13. During interphase of cycle 14, the two clusters colocalise with high frequency, and move from near the midline of the nucleus towards the apical side.
(Hiraoka et al., 1993)
Scaffold attachment region sequences are implicated in the regional opening or closing of chromatin, possibly through their ability to serve as regulators for the His1-induced condensation of chromatin.
(Kas et al., 1993)
In vivo UV cross-linking and nuclear run-on assays shows that RNA polymerase II density on the Hsp70Bb gene is rapidly repressed by heat shock.
(O'Brien and Lis, 1993)
DNA replication of the 5kb histone gene repeating unit in tissue culture cells (Drosophila Kc cells) initiates at multiple sites located within the repeating unit. Several replication pause sites are located at 5' upstream regions of some histone genes.
(Shinomiya and Ina, 1993)
Nascent chain nuclear run-on assays in KC161 cells reveal different responses to heat shock for different genes. Transcription of His1 is severely inhibited under mild heat shocks, of Act5C decreases proportionally with increasing temperature while that of the core histone genes or the heat shock cognates is repressed only under extreme heat shock. Increased transcription of the heat shock genes is observed within 1-2 mins of heat shock and maximal rates were reached within 2-5 minutes. Rates of transcription vary over a 20-fold range.
(Vazquez et al., 1993)
DNaseI footprinting analysis reveals that histone His1, unlike His2A, His2B, His3 and His4, fails to bind to the kni, Kr and Ubx minimal enhancer elements.
(Kerrigan and Kadonaga, 1992)
Comparison of CpG distribution in the coding region of 121 genes from six species supports the mCpG mutational hotspot explanation of CpG suppression in methylated species at position II-III and III-I.
(Schorderet and Gartler, 1992)
His1 is a general repressor of transcription.
(Croston et al., 1991)
The genomic organisation of the histone genes in D.hydei closely resembles that of D.melanogaster.
(Kremer and Hennig, 1990)
The expression of HIS-C genes, including His1, during oogenesis has been studied, and compared to periods of DNA synthesis and actin expression during this developmental stage.
(Ruddell and Jacobs-Lorena, 1985)
4.8kb and 5.0kb repeats containing the histone genes His1, His2A, His2B, His3 and His4 are present in all of the more than 20 D.melanogaster strains studied. The strains differ in the relative amounts of the two repeat types, with the 5.0kb repeat always present in equal or greater amounts than the 4.8kb repeat. The strains also differ in a number of far less abundant fragments containing histone gene sequences.
(Strausbaugh and Weinberg, 1982)
Encodes Histone-H1. See HIS-C record. H1 associates with DNA between nucleosomes. The ratio of H1 to nucleosome core histones is higher in the salivary glands of larvae than in the cells of young embryos (Holmgren, Johansson, Lambertsson, and Rasmusson, 1985). The expression of the histone genes changes in mid-embryogenesis (Ambrosio and Schedl, 1985; Ruddell and Jacobs-Lorena, 1985). The egg chambers contain a variable and low level of mRNA during nurse cell polytenization; however, at the end of stage 10, all the nurse cells accumulate histone mRNA which is turned over to the growing oocytes as the nurse cells degenerate. Heterozygosity for full or partial deficiency of the histone genes suppresses variegation (BSV, Sbv, wm4); duplications without effect on level of variegation (Moore, Sinclair and Grigliatti, 1983). Transcription not repressed by heat shock (Spradling, Pardue and Penman, 1977).
 
hide External Crossreferences & Linkouts
Sequence Crossreferences
DDBJ / EMBL / GenBank
•
UniProtKB/Swiss-Prot
•
UniProtKB/TrEMBL
•
Other Crossreferences
EPD - Eukarytoic Promoter Database, an annotated collection of POL II promoters
•
InterPro domains - A database of protein families, domains, and functional sites
•
Histone H1/H5 (IPR005818)
Histone H5 (IPR005819)
Winged helix-turn-helix transcription repressor DNA-binding (IPR011991)
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
•
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
•
hide Synonyms & Secondary IDs ( 12 )
Reported As
Symbol Synonym
dH1
(Dominski et al., 2005)
DmeH1
(Fitch and Strausbaugh, 1993)
H1
(Camporeale et al., 2007, Ivanovska et al., 2005, Kim, 2004, Pirrotta, 2004, McBryant et al., 2003, Andrioli et al., 2002, Conaway et al., 2002, Wassarman and Sauer, 2001, Yu and Wolfner, 2002, Ner et al., 2001, Berloco et al., 2001, Kleene, 2001, Pham and Sauer, 2000, Fenger et al., 2000, Farkas et al., 2000, Renner et al., 2000, Reim et al., 1999, Chen et al., 1999, Schienman et al., 1998, Pazin et al., 1998, Borgnetto et al., 1999, Karetsou et al., 1998, Belyaeva et al., 1998, Walker and Bownes, 1998, Martinez-Balbas et al., 1998, Hassan and Vaessin, 1997, Strausbaugh and Williams, 1996, Santiago et al., 1995, Karr et al., 1995, Blank and Becker, 1995, Granok et al., 1995, Kamakaka and Kadonaga, 1993, Sandaltzopoulos et al., 1994, Belikov et al., 1993, Belikov et al., 1993, Shinomiya and Ina, 1993, O'Brien and Lis, 1993, Kas et al., 1993, Svaren and Horz, 1993, Kerrigan and Kadonaga, 1992, Becker and Wu, 1992, Wagner et al., 1992, Croston et al., 1992, Kas and Laemmli, 1992, Franke et al., 1992, Croston et al., 1991, Shinomiya and Ina, 1991, Udvardy and Schedl, 1991, Harisanova et al., 1991, Harisanova and Ralchev, 1991, Kremer and Hennig, 1990, Amati and Gasser, 1990, Fitch et al., 1990, Hill et al., 1989, Domier et al., 1986, Ruddell and Jacobs-Lorena, 1985, Maile et al., 2004, Villar-Garea and Imhof, 2008, Ponte et al., 2003, Braunschweig et al., 2009, Lanzotti et al., 2002, Camporeale et al., 2006, Ohsawa et al., 2008, Isogai et al., 2007, Dilworth et al., 2000, Nagel and Grossbach, 2000, Corona et al., 2007, Lu et al., 2009, Shevelyov et al., 2009, Graham et al., 2009, Nagoda et al., 2005, Wang and Kalderon, 2009, Godfrey et al., 2006, Pinnola et al., 2007, Filion et al., 2010, Guillebault and Cotterill, 2007, modENCODE Consortium et al., 2010, Regnard et al., 2011, Lusser et al., 2005, Santoso and Kadonaga, 2006, Egelhofer et al., 2011, van Eyk et al., 2011)
H1A
(Li and Vaessin, 2000)
his
(Matsushima and Kaguni, 2007)
His1
(Markstein et al., 2008, Gilchrist et al., 2008, Sakai et al., 2009, de Wit et al., 2008, Ji et al., 2002, Siriaco et al., 2009)
HisC
(Greil et al., 2003, van Steensel et al., 2001)
hisl
(McVean and Vieira, 1999)
histone
(Corpet and Almouzni, 2007)
Name Synonym
histone
(Matsushima et al., 2004, Kagansky et al., 2004, McKee, 2004, Crevel et al., 2001, McKee et al., 2000, Donaghue et al., 2001, Crevel et al., 2001, Gemkow et al., 2001, Foe et al., 2000, Brent et al., 2000, Marley et al., 2000, Fitch and Wakimoto, 1998, Fung et al., 1998, Troyer et al., 1998, Baker et al., 1993, Machida et al., 2005, Wagner et al., 2008)
Histone
(Parsons et al., 2003)
Histone H1
(Papp and Muller, 2006, Villar-Garea and Imhof, 2008, Roque et al., 2004, Braunschweig et al., 2009, Isogai et al., 2007, Dilworth et al., 2000, Shevelyov et al., 2009, Graham et al., 2009, Siriaco et al., 2009, Wang and Kalderon, 2009)
histone H1
(Ni et al., 2006, Horner et al., 2006, Großhans et al., 2007, Lanzotti et al., 2002, Kent, 2008, Boehm et al., 2003, Nagel and Grossbach, 2000, Corona et al., 2007, Lu et al., 2009, Wang and Kalderon, 2009, Pinnola et al., 2007, Ji et al., 2002)
Secondary FlyBase IDs
hide References ( 172 )
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hide Recent research papers ( 5 )
Egelhofer et al., 2011, Nat. Struct. Mol. Biol. 18(1): 91--93
An assessment of histone-modification antibody quality. [FBrf0212707]
Regnard et al., 2011, PLoS Genet. 7(3): e1001327
Global Analysis of the Relationship between JIL-1 Kinase and Transcription. [FBrf0213276]
van Eyk et al., 2011, Hum. Mol. Genet. 20(14): 2783--2794
Perturbation of the Akt/Gsk3-{beta} signalling pathway is common to Drosophila expressing expanded untranslated CAG, CUG and AUUCU repeat RNAs. [FBrf0213942]
Filion et al., 2010, Cell 143(2): 212--224
Systematic protein location mapping reveals five principal chromatin types in Drosophila cells. [FBrf0212051]
modENCODE Consortium et al., 2010, Science 330(6012): 1787--1797
Identification of functional elements and regulatory circuits by Drosophila modENCODE. [FBrf0212741]
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