H2A, histone, core histone, HisC, H2A.1
Chromatin component - a core histone that can be modified by ubiquitination - a target of polycomb gene silencing - involved in trans-histone regulation through histone H3 - H2A monoubiquitination promotes histone H3 methylation in Polycomb repression
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
The chromatin-associated form, but not the free cytoplasmic form, is phosphorylated on Thr-120 by NHK-1 during mitosis, and dephosphorylated during S-phase. Also phosphorylated on Thr-120 by NHK-1 during prophase I of meiosis; which is required for acetylation of H3 'Lys-14' and H4 'Lys-5', diassembly of the synaptonemal complex, and karyosome formation.
Monoubiquitination of Lys-119 by sce/dRING gives a specific tag for epigenetic transcriptional repression.
Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 directly represses transcription (By similarity).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\His2A using the Feature Mapper tool.
His4 and His2A that was mono or poly acetylated is detected associated with nuclei of developing spermatids until late stages. During late spermatid development and in mature spermatazoa these proteins can no longer be detected (and are presumably replaced by sperm specific chromatin packaging proteins). However, shortly after fertilization His4 and His2A immunoreactivity reappears associated with the male pronuclear DNA.
GBrowse - Visual display of RNA-Seq signalsView Dmel\His2A in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
The ATPase activity of Iswi is completely inhibited by each of the four histone tails (His2A, His2B, His3 and His4), results indicate a novel role for the flexible histone tails in chromatin remodeling by Iswi.
The codon bias of the histone genes from D.melanogaster and D.hydei illustrates that the generalisation that abundantly expressed genes have a high codon bias and low rates of silent substitution does not hold for the histone genes.
The position of the homologous histone gene repeats within the nuclei of early embryo cells has been investigated. The two homologous histone gene clusters are distinct and separate through all stages of the cell cycle up to nuclear cycle 13. During interphase of cycle 14, the two clusters colocalise with high frequency, and move from near the midline of the nucleus towards the apical side.
DNA replication of the 5kb histone gene repeating unit in tissue culture cells (Drosophila Kc cells) initiates at multiple sites located within the repeating unit. Several replication pause sites are located at 5' upstream regions of some histone genes.
One H2A-like sequence variant has been found (Donahue, Palmer, Condie, Sabatini, and Blumenfeld, 1986); see His2Av.
Encodes Histone-2A. See HIS-C record.