H2Av, H2AvD, His2AvD, H2A.Z, H2A.V
replacement histone participates in an ordered cascade of events leading to the establishment of heterochromatin - controls poly(ADP-ribose) polymerase 1 (PARP1) activation in chromatin
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant His2Av forms a heterodimer with H2B.
Phosphorylated. Phosphorylation of Ser-138 occurs in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents. Phosphorylation is dependent on the DNA damage checkpoint kinases ATR and ATM, spreads on either side of a detected DSB site and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair.
Acetylated on Lys-5 by Tip60. Acetylation is enhanced by Ser-138 phosphorylation and promotes the exchange of the phosphorylated form with the unmodified form of H2AV.
Monoubiquitination of Lys-121 by sce/dRING gives a specific tag for epigenetic transcriptional repression.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\His2Av using the Feature Mapper tool.
His2Av is detected in the germarium and in the nucleus of the oocyte and nurse cells as well as mitotically-dividing follicle cells until oogenesis stage S3. After this, staining is dramatically reduced in the nurse cells and oocyte but persists in the follicle cells.
GBrowse - Visual display of RNA-Seq signalsView Dmel\His2Av in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: His2Av l(3)05146
Phosphorylation of His2Av is critical for fly viability after gamma-irradiation.
The C terminal region of His2Av, excluding the last 12 amino acids, is critical for function. This region is buried inside the histone core and is not responsible for interactions with DNA, but is possibly involved in protein-protein interactions.
In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
His2Av function is essential for nucleosome assembly and is continuously expressed.
Isolated from a cDNA library, using a Tetrahymena histone H2A variant hv1 cDNA as a probe.