Gene Dmel\His2B

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General Information

Symbol

Dmel\His2B

Species

D. melanogaster

Name

Histone H2B

Annotation symbol

Feature type

protein_coding_gene

FlyBase ID

FBgn0001198

Gene Model Status

Not Applicable

Stock availability

None publicly available

Also Known As

H2B, dH2B, HisC

Genomic Location

Chromosome (arm)

Recombination map

2-55

Cytogenetic map

39D3-39E1

Sequence location

Summary Information

Automatically generated summary

See sections below for more information

The gene Histone H2B is referred to in FlyBase by the symbol Dmel\His2B (FBgn0001198). This gene record represents a gene family, individual members of the family are: CG17949, CG33868, CG33870, CG33872, CG33874, CG33876, CG33878, CG33880, CG33882, CG33884, CG33886, CG33888, CG33890, CG33892, CG33894, CG33896, CG33898, CG33900, CG33902, CG33904, CG33906, CG33908, CG33910, CR40461. Based on sequence similarity, it is predicted to have molecular function: DNA binding. It is reported to be involved in the biological process: chromatin assembly or disassembly. 11 alleles are reported. No phenotypic data is available. It has no annotated transcripts. Protein features are: Histone H2B; Histone core; Histone-fold. Gene has not been localized to the genome sequence.

User Contributed Data

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Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

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Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

39D3-39E1

Left limit from in situ hybridisation (FBrf0029738) Right limit from molecular mapping relative to His2A (FBrf0044950)

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

39D-39E

(determined by in situ hybridisation)

(Fitch et al., 1990)

39D3-39E2

(determined by in situ hybridisation)

(Pardue et al., 1977)

Experimentally Determined Recombination Data

Location

2-55

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Histone H2B (IPR000558)

Histone core (IPR007125)

Histone-fold (IPR009072)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P02283

UniProtKB/TrEMBL

Mapped Features

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 14

central nervous system

(Lanzotti et al., 2004, Lanzotti et al., 2002)

midgut

(Lanzotti et al., 2004)

midgut | restricted

(Lanzotti et al., 2002)

Comment:endoreplicating cells

Additional Descriptive Data

His2B transcript is restricted to replicating cells.

(Lanzotti et al., 2002)

Marker for

mitotic domain

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

modENCODE Anatomy RNA-Seq

modENCODE Development RNA-Seq

modENCODE Cell Lines RNA-Seq

modENCODE Treatments RNA-Seq

Expression Clusters

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0001198

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of His2B	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Allele of His2B	Mutagen	Known lesion
His2B^{Act5C.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	--
His2B^{Act5C.T:Avic\GFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	Yes
His2B^{cid.T:Avic\GFP}	in vitro construct - coding region fusion in vitro construct - regulatory fusion	Yes
His2B^HisGU.wt		Yes
His2B^{hs.T:Avic\GFP}	in vitro construct - coding region fusion in vitro construct - regulatory fusion	Yes
His2B^{MtnA.T:Avic\GFP}	in vitro construct - coding region fusion	Yes
His2B^{Scer\UAS.cZa.T:Disc\RFP-mRFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	Yes
His2B^{Scer\UAS.P\T.T:Avic\GFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	Yes
His2B^{Scer\UAS.T:Avic\GFP-ECFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	Yes
His2B^{Scer\UAS.T:Disc\RFP-mRFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	Yes
His2B^{Scer\UAS.T:Zzzz\KO-mKO}	FLPase	Yes

Aneuploid Aberrations

Disrupted in

Df(2L)C239

(Moore et al., 1981)

Df(2L)His^C

(Günesdogan et al., 2010)

Duplicated in

Dp(2;1)C239

(Moore et al., 1981)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{UAS-mRFP-His2B.Z}

P{UASp(FRT.His2B-GFP)His2B-mKO}

P{UASp(-FRT)His2B-mKO}

heat-shock construct

P{hs-His2B.GFP}

characterization construct

Insertions

Type of insertions

Name

Expression data

Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 6 terms )

Molecular Function

CV term

Evidence

References

DNA binding

inferred from sequence or structural similarity with UniProtKB:P04255

(FlyBase, 1992-)

non-traceable author statement

(Swiss-Prot Project Members, 1986.7.21)

protein heterodimerization activity

inferred from electronic annotation with InterPro:IPR009072

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

chromatin assembly or disassembly

non-traceable author statement

(Swiss-Prot Project Members, 1986.7.21)

nucleosome assembly

inferred from electronic annotation with InterPro:IPR000558

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

Evidence

References

nucleosome

inferred from sequence or structural similarity with UniProtKB:P04255

(FlyBase, 1992-)

non-traceable author statement

(Swiss-Prot Project Members, 1986.7.21)

traceable author statement

(Leach et al., 2000)

nucleus

inferred from electronic annotation with InterPro:IPR000558

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

member_gene_array

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

His2B - scny

anti bait coimmunoprecipitation, western blot

(Buszczak et al., 2009)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

His2B allele

Gene

References

External Data

Linkouts

Orthologs

OrthoDB Orthologs (0) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

No orthologs identified

Dipteran inclusive ortholog search

No orthologs identified

Insect inclusive ortholog search

No orthologs identified

Arthropod inclusive ortholog search

No orthologs identified

Metazoa inclusive ortholog search

No orthologs identified

Orthologs in Drosophila Species (None identified)

No orthologies identified

Orthologs in non-Drosophila Dipterans (None identified)

No non-Drosophilid orthologies identified

Orthologs in non-Dipteran Insects (None identified)

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (None identified)

No non-Insect Arthropod orthologies identified

Orthologs in non-Arthropod Metazoa (None identified)

No non-Arthropod Metazoa orthologies identified

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (0) based on analysis using Dmel annotation version 4.3

No orthologs identified

Stocks & Reagents

Stocks Listed in FlyBase ( 0 )

Genomic Clones ( 0 )

cDNA Clones ( 0 )

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

Antibody Information

monoclonal

(Egelhofer et al., 2011)

polyclonal

(Egelhofer et al., 2011)

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Encoded by

Component gene(s)

Additional comments

Other Comments

The majority of replication-dependent histone gene transcripts are not polyadenylated and in addition two types of polyadenylated transcripts can be detected. A small proportion of the histone mRNAs bear a short poly(A) tail which is added to the 3' terminus of a partially degraded stem-loop structure. Polyadenylation signals can be located downstream of the stem-loop structure that can be used to generate mRNAs with a poly(A) tail.

(Akhmanova et al., 1997)

The ATPase activity of Iswi is completely inhibited by each of the four histone tails (His2A, His2B, His3 and His4), results indicate a novel role for the flexible histone tails in chromatin remodeling by Iswi.

(Georgel et al., 1997)

The position of the homologous histone gene repeats within the nuclei of early embryo cells has been investigated. The two homologous histone gene clusters are distinct and separate through all stages of the cell cycle up to nuclear cycle 13. During interphase of cycle 14, the two clusters colocalise with high frequency, and move from near the midline of the nucleus towards the apical side.

(Hiraoka et al., 1993)

DNA replication of the 5kb histone gene repeating unit in tissue culture cells (Drosophila Kc cells) initiates at multiple sites located within the repeating unit. Several replication pause sites are located at 5' upstream regions of some histone genes.

(Shinomiya and Ina, 1993)

DNaseI footprinting analysis reveals core histones His2A, His2B, His3 and His4 (but not His1) bind to the kni, Kr and Ubx minimal enhancer elements in a periodic manner.

(Kerrigan and Kadonaga, 1992)

The genomic organisation of the histone genes in D.hydei closely resembles that of D.melanogaster.

(Kremer and Hennig, 1990)

The D.virilis core histone genes (Dvir\His2B, Dvir\His3, Dvir\His4 and Dvir\His2A), are arranged in the same order and orientation as the D.melanogaster core histone genes (His2B, His3, His4 and His2A). However, the His1 gene that is located between His2B and His3 in D.melanogaster is not found between Dvir\His2B and Dvir\His3 in D.virilis. Overall orientation not stated: His2B+ His1- His3- His4+ His2A-

(Domier et al., 1986)

External Crossreferences & Linkouts

Sequence Crossreferences

DDBJ / EMBL / GenBank

•

UniProtKB/Swiss-Prot

•

P02283

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Histone H2B (IPR000558)

Histone core (IPR007125)

Histone-fold (IPR009072)

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0001198

Synonyms & Secondary IDs ( 20 )

Reported As

Symbol Synonym

dH2B

(Hoffmann et al., 1996, Dominski et al., 2005)

dH2b

(Yang et al., 2009)

DmeH2b

(Fitch and Strausbaugh, 1993)

H2_B

(Amati and Gasser, 1990)

H2B

(Cakouros et al., 2008, Camporeale et al., 2007, Raisner, 2006, van der Knaap et al., 2005, Kusch et al., 2004, Kusch et al., 2004, Schwartz and Ahmad, 2005, Dudnik et al., 2005, Morales et al., 2004, Lippman and Martienssen, 2004, Aihara et al., 2004, Beach and Wolfner, 2004, Smith, 2002, Cao et al., 2002, Blower and Karpen, 2002, Yu and Wolfner, 2002, Levenstein and Kadonaga, 2002, Hamiche et al., 2001, Berloco et al., 2001, Katsani et al., 2001, Nakagawa et al., 2001, Mello and Almouzni, 2001, Ahmad and Henikoff, 2001, Mizuguchi et al., 2001, Mizzen and Allis, 2000, Pham and Sauer, 2000, Leach et al., 2000, Verreault, 2000, Kal et al., 2000, Baldo et al., 1999, Hamiche et al., 1999, Hennig, 1999, Carrier et al., 1999, Schienman et al., 1998, Borgnetto et al., 1999, Smith et al., 1998, Walker and Bownes, 1998, Strausbaugh et al., 1998, Grunstein, 1997, Georgel et al., 1997, Akhmanova et al., 1997, Strausbaugh and Williams, 1996, Sobel et al., 1994, Sommer and Strausbaugh, 1996, Blank and Becker, 1995, Shinomiya and Ina, 1993, O'Brien and Lis, 1993, Kerrigan and Kadonaga, 1992, Becker and Wu, 1992, Pauli et al., 1992, Kas and Laemmli, 1992, O'Brien and Lis, 1991, Udvardy and Schedl, 1991, Harisanova et al., 1991, Harisanova and Ralchev, 1991, Kremer and Hennig, 1990, Fitch et al., 1990, Domier et al., 1986, Mendjan et al., 2006, Goshima et al., 2007, Jaklevic et al., 2006, Furuyama et al., 2006, Hickson et al., 2006, Ni et al., 2006, Tran et al., 2012, Zobeck et al., 2010, Goshima et al., 2007, Brasaemle and Hansen, 2006, Buszczak et al., 2008, Weake et al., 2008, Camporeale et al., 2006, Isogai et al., 2007, Chen et al., 2011, Lorbeck et al., 2011, Kolesnikova et al., 2009, Heun et al., 2006, Buttrick et al., 2008, Carreno et al., 2008, Erhardt et al., 2008, Dalal et al., 2007, Corona et al., 2007, Dalal et al., 2007, de Vries et al., 2005, Buszczak et al., 2009, Ito et al., 2012, Brower-Toland et al., 2009, Bhattacharya et al., 2009, Sullivan et al., 2009, Nie et al., 2009, Li et al., 2012, Sakai et al., 2009, Weake et al., 2009, Godfrey et al., 2009, Moshkin et al., 2009, Morciano et al., 2009, Simon et al., 2009, Sawatsubashi et al., 2010, Mohan et al., 2010, Mazumder and Shivashankar, 2010, Lee et al., 2010, Scheuermann et al., 2010, Deal et al., 2010, Pinnola et al., 2007, Villar-Garea et al., 2012, modENCODE Consortium et al., 2010, Zobeck et al., 2010, Bao et al., 2008, Banerjee et al., 2006, Regnard et al., 2011, Kuranaga et al., 2011, Li et al., 2012, Lusser et al., 2005, Santoso and Kadonaga, 2006, Egelhofer et al., 2011, Wang et al., 2013, Makde et al., 2010, Krajewski, 2008, Clapier et al., 2008, Petruk et al., 2012, Zhou et al., 2012, Olins et al., 2011, Bonn et al., 2012, Xie et al., 2012)

H2b

(Rodriguez-Trelles et al., 2000, Shinomiya and Ina, 1991, Ruddell and Jacobs-Lorena, 1985, Dominski et al., 2002, Adamson and Price, 2003, Lanzotti et al., 2002, Godfrey et al., 2006, van der Knaap et al., 2010)

his

(Matsushima and Kaguni, 2007)

His2b

(Shopland and Lis, 1996)

His2B

(Günesdogan et al., 2010, Gilchrist et al., 2008, Cabernard and Doe, 2009, Bauer et al., 2010, Kozhevnikova et al., 2012)

HisC

(Greil et al., 2003, van Steensel et al., 2001)

histone

(Corpet and Almouzni, 2007)

Name Synonym

core histone

(Xia et al., 2003, Livengood et al., 2002)

Histone

(Parsons et al., 2003, Breiling et al., 1999)

histone 2B

(Finley et al., 2003, Maile et al., 2004)

Histone2B

(Ishida et al., 2012, Koto et al., 2009)

Histone 2B

(Kuranaga et al., 2011)

Histone H2B

histone H2B

(Griffis et al., 2007, Ringrose and Paro, 2007, Buszczak et al., 2009, Scheuermann et al., 2010, Bao et al., 2008)

histone H2b

(Lanzotti et al., 2002)

histone h2b

(Larschan et al., 2012)

Secondary FlyBase IDs

FBgn0039974

References ( 231 )

Generate a list of

All references relating to this gene ( 231 )

List References by type

Recent research papers ( 20 )

Wang et al., 2013, Nat. Struct. Mol. Biol. 20(2): 202--209: Chromatin proteins captured by ChIP-mass spectrometry are linked to dosage compensation in Drosophila. [FBrf0220693]
Anand et al., 2012, eLife 1: e00003: A novel role for lipid droplets in the organismal antibacterial response. [FBrf0219996]
Bonn et al., 2012, Nat. Protoc. 7(5): 978--994: Cell type-specific chromatin immunoprecipitation from multicellular complex samples using BiTS-ChIP. [FBrf0218169]
Hödl and Basler, 2012, Curr. Biol. 22(23): 2253--2257: Transcription in the Absence of Histone H3.2 and H3K4 Methylation. [FBrf0220201]
Ishida et al., 2012, PLoS ONE 7(2): e30265: Prevention of Apoptosis by Mitochondrial Phosphatase PGAM5 in the Mushroom Body Is Crucial for Heat Shock Resistance in Drosophila melanogaster. [FBrf0217492]
Ito et al., 2012, Mol. Cell 45(4): 494--504: Epigenetic Silencing of Core Histone Genes by HERS in Drosophila. [FBrf0217607]
Kozhevnikova et al., 2012, Mol. Cell 47(1): 133--139: Metabolic Enzyme IMPDH Is Also a Transcription Factor Regulated by Cellular State. [FBrf0218906]
Larschan et al., 2012, PLoS Genet. 8(7): e1002830: Identification of Chromatin-Associated Regulators of MSL Complex Targeting in Drosophila Dosage Compensation. [FBrf0219020]
Li et al., 2012, Curr. Biol. 22(22): 2104--2113: Lipid droplets control the maternal histone supply of Drosophila embryos. [FBrf0220040]
Petruk et al., 2012, Cell 150(5): 922--933: TrxG and PcG Proteins but Not Methylated Histones Remain Associated with DNA through Replication. [FBrf0219341]
Tran et al., 2012, Science 338(6107): 679--682: Asymmetric division of Drosophila male germline stem cell shows asymmetric histone distribution. [FBrf0219854]
Villar-Garea et al., 2012, Nucleic Acids Res. 40(4): 1536--1549: Developmental regulation of N-terminal H2B methylation in Drosophila melanogaster. [FBrf0217560]
Xie et al., 2012, Mol. Cell. Proteomics 11(5): 100--107: Lysine succinylation and lysine malonylation in histones. [FBrf0218318]
Zhou et al., 2012, J. Mol. Biol. 421(1): 30--37: Histone H4 K16Q mutation, an acetylation mimic, causes structural disorder of its N-terminal basic patch in the nucleosome. [FBrf0219800]
Chen et al., 2011, J. Biol. Chem. 286(11): 9020--9030: E2 Ligase dRad6 Regulates DMP53 Turnover in Drosophila. [FBrf0213391]
Egelhofer et al., 2011, Nat. Struct. Mol. Biol. 18(1): 91--93: An assessment of histone-modification antibody quality. [FBrf0212707]
Kuranaga et al., 2011, Development 138(8): 1493--1499: Apoptosis controls the speed of looping morphogenesis in Drosophila male terminalia. [FBrf0213320]
Lorbeck et al., 2011, PLoS ONE 6(4): e18412: Microarray analysis uncovers a role for tip60 in nervous system function and general metabolism. [FBrf0213446]
Olins et al., 2011, Nucleus 2(1): 47--60: An epichromatin epitope: persistence in the cell cycle and conservation in evolution. [FBrf0218029]
Regnard et al., 2011, PLoS Genet. 7(3): e1001327: Global Analysis of the Relationship between JIL-1 Kinase and Transcription. [FBrf0213276]

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Recent reviews (0)

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