FlyBase Gene Report: Dmel\hook

General Information

Symbol

Dmel\hook

Species

D. melanogaster

Name

hook

Annotation Symbol

CG10653

Feature Type

protein_coding_gene

FlyBase ID

FBgn0001202

Gene Model Status

Current

Stock Availability

75 publicly available

Gene Summary

Involved in endocytic trafficking by stabilizing organelles of the endocytic pathway. Probably acts as a cytoskeletal linker protein required to tether endosome vesicles to the cytoskeleton. Involved in modulation of endocytosis at stages required for down-regulation of membrane proteins that control synapse size. Not involved in synaptic vesicle recycling. Required in R7 cells for boss endocytosis into multivesicular bodies (MVBs). Has a role in regulating adult longevity. (UniProt, Q24185)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
UniProtKB
Red Book
Interactive Fly

Also Known As

Key Links

Genomic Location

Cytogenetic map

37B10-37B10

Sequence location

2L:19,030,844..19,033,498 [-]

Recombination map

2-54

RefSeq locus

NT_033779 REGION:19030844..19033498

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (11 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables microtubule binding

inferred from direct assay

(assigned by UniProt )

(Walenta et al., 2001)

enables small GTPase binding

inferred from physical interaction with FLYBASE:Rab5; FB:FBgn0014010

(Gillingham et al., 2014)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables dynein light intermediate chain binding

inferred from biological aspect of ancestor with PANTHER:PTN000436622

(assigned by GO_Central )

(Gaudet et al., 2011)

enables microtubule binding

inferred from biological aspect of ancestor with PANTHER:PTN000436622

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in cytoskeleton-dependent intracellular transport

inferred from direct assay

(assigned by UniProt )

(Walenta et al., 2001)

involved_in determination of adult lifespan

inferred from mutant phenotype

(Simonsen et al., 2007)

involved_in endocytosis

inferred from expression pattern

(assigned by UniProt )

(Kramer and Phistry, 1999)

inferred from expression pattern

(Kramer and Phistry, 1996)

inferred from mutant phenotype

(Kramer and Phistry, 1996)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in cytoplasmic microtubule organization

inferred from biological aspect of ancestor with PANTHER:PTN000436622

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in cytoskeleton-dependent intracellular transport

inferred from biological aspect of ancestor with PANTHER:PTN000436622

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in endocytosis

traceable author statement

(Luzio et al., 2000)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in endosome

inferred from direct assay

(assigned by UniProt )

(Walenta et al., 2001)

located_in synapse

inferred from mutant phenotype

(Narayanan et al., 2000)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in centrosome

inferred from biological aspect of ancestor with PANTHER:PTN000436622

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000436622

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in cytoplasm

non-traceable author statement

(Luzio et al., 2000)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the hook family. (Q24185)

Protein Signatures (InterPro)

Summaries

Protein Function (UniProtKB)

(UniProt, Q24185)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

hk: hook

hk: hook

From Mohr, 1927, Hereditas 9: 169-79.

Bristles nearly all hooked at tip or blunted, some bent at right angles. Scutellars and verticals especially affected. Acrostichal hairs fewer and outer rows separated. Eyes slightly roughened. Wings usually divergent and may be smaller. Body sometimes small and chunky. Less extreme expression at 19, especially the wing character, but classification reliable. RK2.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\hook for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q24185)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

hook-RA

FBtr0081180

NM_057225

2317

679

Additional Transcript Data and Comments

Reported size (kB)

2.3 (longest cDNA)

(Kramer and Phistry, 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

hook-PA

FBpp0080722

76.7

679

4.99

NP_476573

AAF53742

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

679 (aa); 85 (kD observed); 77 (kD predicted)

(Kramer and Phistry, 1996)

Comments

External Data

Subunit Structure (UniProtKB)

Homodimer. Interacts with microtubules via its N-terminus.

(UniProt, Q24185)

Domain

The coiled coil domain mediates homodimerization.

(UniProt, Q24185)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\hook using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval hemocyte

• phagocytic vesicle

(Rahman et al., 2012)

pupal stage P7

macrochaeta | restricted

Comment: reference states 44 hours APF

(Bitan et al., 2010)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Kramer and Phistry, 1996)

adult stage

organism

(Rahman et al., 2012)

Additional Descriptive Data

The multivesicular bodies in which hk protein is detected are a component of the general endocytic compartment. In embryos, cortical areas of cells stain for hk protein in a punctate pattern. In the oocyte, a similar cortical localization is detected, while in the nurse cell, staining is even throughout the cytoplasm.

(Rahman et al., 2012, Kramer and Phistry, 1996)

hk protein localization is restricted to the perimeter of large endosomes in Schneider2:Sev cells, while dor protein is found internally as well.

(Bitan et al., 2010, Sevrioukov et al., 1999)

The hk protein is detected in all developmental stages, from the embryo to the adult.

(Kramer and Phistry, 1996)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in endosome

inferred from direct assay

(assigned by UniProt )

(Walenta et al., 2001)

located_in synapse

inferred from mutant phenotype

(Narayanan et al., 2000)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\hook in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\hook

View exonic expression by tissue for Dmel\hook

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001202

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35169/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001202

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001202

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001202

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10653

Flygut - An atlas of the Drosophila adult midgut

FBgn0001202

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 17 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of hook

Transgenic constructs containing regulatory region of hook

Aberrations (Deficiencies and Duplications) ( 56 )

Inferred from experimentation ( 56 )

Gene disrupted in

Df(2L)E71

(Wright et al., 1981, Wright et al., 1976)

(Ryder, 2004.4.26)

(Ryder, 2004.4.26)

(Wright, 1996, Pentz et al., 1986, Brittnacher and Ganetzky, 1983)

Df(2L)hk39

(Brittnacher and Ganetzky, 1983)

Df(2L)hk-UC1

(Wright, 1996, Stathakis et al., 1995)

Df(2L)hk-UC2

(Wright, 1996, Stathakis et al., 1995)

Df(2L)OD15

(Thor and Thomas, 1997, Wright, 1996, Stathakis et al., 1995, Pentz et al., 1986)

Df(2L)Sd68

(Brittnacher and Ganetzky, 1983)

Df(2L)TW50

(Wright et al., 1976)

Df(2L)TW130

(Kramer and Phistry, 1999, Kramer and Phistry, 1996, Wright, 1996, Stathakis et al., 1995, Wright et al., 1981, Wright et al., 1976)

Df(2L)TW158

(Wright et al., 1976)

Df(2L)TW203

(Wright, 1996, Stathakis et al., 1995, Pentz et al., 1986, Wright et al., 1981)

Df(2L)VA18

(Wright, 1996, Stathakis et al., 1995, Pentz et al., 1986, Gilbert et al., 1984)

Df(2L)VA21

(Wright, 1996, Stathakis et al., 1995)

Df(2L)VA23

(Stathakis et al., 1995)

Ts(Y;2Lt)D219+Ts(Y;2Rt)H174

(Wright et al., 1976)

Gene duplicated in

Dp(2;1)C239

(Matthews, 2004.12.29)

Dp(2;Y)G-M15

(Contamine et al., 1989)

Dp(2;Y)H

Gene not disrupted in

Df(2L)E55

(Stathakis et al., 1995, Wright et al., 1976)

Df(2L)NST

(Wright, 1996, Stathakis et al., 1995, Pentz et al., 1986)

Df(2L)pr21

(Brittnacher and Ganetzky, 1983)

Df(2L)pr26

(Brittnacher and Ganetzky, 1983)

Df(2L)pr47

(Brittnacher and Ganetzky, 1983)

Df(2L)pr49

(Brittnacher and Ganetzky, 1983)

Df(2L)pr65

(Brittnacher and Ganetzky, 1983)

Df(2L)pr67

(Brittnacher and Ganetzky, 1983)

Df(2L)pr69

(Brittnacher and Ganetzky, 1983)

Df(2L)pr-B12

(Brittnacher and Ganetzky, 1983)

Df(2L)Sd2

(Brittnacher and Ganetzky, 1983)

Df(2L)Sd14

(Brittnacher and Ganetzky, 1983)

Df(2L)Sd37

(Wright, 1996, Stathakis et al., 1995, Brittnacher and Ganetzky, 1983)

Df(2L)Sd57

(Wright, 1996, Stathakis et al., 1995, Brittnacher and Ganetzky, 1983)

Df(2L)Sd77

(Wright, 1996, Stathakis et al., 1995, Brittnacher and Ganetzky, 1983)

Df(2L)TE37C-1

(Wright, 1996, Gilbert et al., 1984, Wright et al., 1981)

Df(2L)TE37C-7

(Wright, 1996, Stathakis et al., 1995)

Df(2L)TW1

(Wright et al., 1976)

Df(2L)TW2

(Wright et al., 1976)

Df(2L)TW3

(Thor and Thomas, 1997, Wright et al., 1976)

Df(2L)TW9

(Wright et al., 1976)

Df(2L)TW12

(Wright et al., 1976)

Df(2L)TW65

(Wright et al., 1976)

Df(2L)TW84

(Wright et al., 1976)

Df(2L)TW119

(Wright et al., 1976)

Df(2L)TW137

(Stathakis et al., 1995)

Df(2L)TW150

(Wright et al., 1976)

Df(2L)TW161

(Wright et al., 1976)

Df(2L)VA12

(Wright, 1996, Stathakis et al., 1995, Pentz et al., 1986, Gilbert et al., 1984, Wright et al., 1981)

Df(2L)VA13

(Wright, 1996, Gilbert et al., 1984, Wright et al., 1981)

Df(2L)VA17

(Wright, 1996, Stathakis et al., 1995, Pentz et al., 1986, Gilbert et al., 1984)

Df(2L)VA19

(Wright, 1996, Stathakis et al., 1995)

Dp(2;Y)H1

(Hodgetts, 1980)

Dp(2;Y)H2

(Hodgetts, 1980)

Dp(2;Y)H3

(Hodgetts, 1980)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

hook^GD12598

viable, with Scer\GAL4^Mef2.PR

hook^GD12598

viable, with Scer\GAL4^pnr-MD237

hook^GD12598

viable, with Scer\GAL4^tin.CΔ4

hook^GD12598

Sterility

Allele

fertile

Other Phenotypes

Allele

abnormal neuroanatomy

abnormal neuroanatomy, with Scer\GAL4^da.G32

hook^GD12598

abnormal neurophysiology

hook¹¹

short lived | male

hook¹

short lived | male (with hook¹)

hook¹¹

short lived | male (with hook¹¹)

hook¹

visible

visible | recessive

wild-type

Phenotype manifest in

Allele

bouton | increased number

hook¹¹

endosome

eye disc

hook¹

eye disc, with Scer\GAL4^da.G32

hook^GD12598

glial cell

hook¹

glial cell, with Scer\GAL4^da.G32

hook^GD12598

lysosome

macrochaeta

macrochaeta & head

macrochaeta & thorax

multivesicular body

ommatidium

synapse

hook¹¹

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\HOOK3

14 of 15

Yes

Hsap\HOOK1

10 of 15

Yes

Hsap\HOOK2

10 of 15

Yes

Hsap\CCDC88B

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Hook3

14 of 15

Yes

Mmus\Hook2

10 of 15

Yes

Mmus\Hook1

9 of 15

Yes

Mmus\Ccdc88b

1 of 15

Rattus norvegicus (Norway rat) (4)

Rnor\Hook1

10 of 13

Yes

Rnor\Hook2

10 of 13

Yes

Rnor\Hook3

4 of 13

Yes

Rnor\Ccdc88b

1 of 13

Xenopus tropicalis (Western clawed frog) (3)

Xtro\hook2

9 of 12

Yes

Xtro\hook3

8 of 12

Yes

Xtro\hook1

6 of 12

Yes

Danio rerio (Zebrafish) (3)

Drer\hook1

11 of 15

Yes

Drer\hook2

6 of 15

Yes

Drer\hook3

2 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\zyg-12

6 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (1)

Gene Symbol

Score

Source

Alignment

2 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

hook¹¹

exacerbates chromosome 3-linked frontotemporal dementia

modeled by Hsap\CHMP2B^Intron5.UAS

(Lu et al., 2020)

hook⁷

exacerbates chromosome 3-linked frontotemporal dementia

modeled by Hsap\CHMP2B^Intron5.UAS

(Lu et al., 2020)

hook^GD12598

exacerbates chromosome 3-linked frontotemporal dementia

modeled by Hsap\CHMP2B^Intron5.UAS

(Lu et al., 2020)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

HOOK3; hook microtubule tethering protein 3

14 of 15

607825

HOOK1; hook microtubule tethering protein 1

10 of 15

607820

HOOK2; hook microtubule tethering protein 2

10 of 15

607824

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

hook - CG14556

experimental knowledge based

(Guruharsha et al., 2011)

hook - Rab11

anti tag coimmunoprecipitation, anti tag western blot, western blot

(Szatmári et al., 2014)

hook - Rab5

pull down, western blot, peptide massfingerprinting

(Gillingham et al., 2014, Gillingham et al., 2014, Gillingham et al., 2014)

hook - peo

experimental knowledge based

(Guruharsha et al., 2011)

hook - spn-F

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Lu et al., 2020)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

hook

suppressible

dor

(Narayanan et al., 2000)

hook

enhanceable

dor

(Narayanan et al., 2000)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

baz

enhanceable

hook

(Shao et al., 2010)

bchs

enhanceable

hook

(Simonsen et al., 2007)

dor

enhanceable

hook

(Narayanan et al., 2000)

shi

enhanceable

hook

(Narayanan et al., 2000)

External Data

Subunit Structure (UniProtKB)

Homodimer. Interacts with microtubules via its N-terminus.

(UniProt, Q24185 )

Linkouts

BioGRID - A database of protein and genetic interactions.

61158

DroID - A comprehensive database of gene and protein interactions.

FBgn0001202

MIST (genetic) - An integrated Molecular Interaction Database

hook

MIST (protein-protein) - An integrated Molecular Interaction Database

hook

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-54

Cytogenetic map

37B10-37B10

Sequence location

2L:19,030,844..19,033,498 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

37B10-37B10

Limits computationally determined from genome sequence between P{lacW}l(2)37Db^k16106&P{lacW}Catsup^k05424 and P{lacW}Ddc^k02104

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

37B10-37B10

(determined by in situ hybridisation)

(Kramer and Phistry, 1996)

Experimentally Determined Recombination Data

Location

2-54

(FlyBase, 2016)

2-53.6

(Comeron et al., 2012)

2-53.9

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (75)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

307

hook¹ pr¹

65858

w^*; P{UASp-hook.GFP}attP2

360

Dp(1;2;1)AT/+; hook¹ Ddc⁷

3184

amos^Tft hook¹ pr¹/CyO

Kyoto

105848

hook¹

105873

Dp(1;2;1)AT/+; hook¹ Ddc⁷

106328

rdo¹ hook¹ pr¹ cn¹

106894

Dp(2;1)C239/+; hook¹ Ddc⁷ pr¹

107544

brat¹ rdo¹ hook¹ pr¹/CyO

107700

scw^l1 rdo¹ hook¹ pr¹/CyO

VDRC

v35483

w¹¹¹⁸; P{GD12598}v35483

v35485

w¹¹¹⁸; P{GD12598}v35485/TM3

More stocks available...

Genomic Clones (27)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (41)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD05265

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0001202

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0001202

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35169

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Kramer and Phistry, 1996)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: hook hk

(FlyBase, 2009-)

Source for database merge of

Additional comments

Renamed from 'hk' to 'hook' to reduce confusion with the 'Hk' ('Hyperkinetic') gene symbol, from which it differed only by case.

(FlyBase, 2009-)

Other Comments

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

hk is required for the normal endocytic trafficking of different classes of ligands.

(Kramer and Phistry, 1999)

hk is required late in endocytic trafficking to negatively regulate delivery from mature multivesicular bodies to multilammelar late endosomes and lysosomes.

(Sunio et al., 1999)

Mutants do not exhibit defects in the denticle belt of hairs of the larvae.

(Dickinson and Thatcher, 1997)

Mutations in hk inhibit the accumulation of boss protein in multivesicular bodies in R7 cells. hk is molecularly characterised and results indicate the hk protein is a novel component of the endocytic compartment.

(Kramer and Phistry, 1996)

hk protein constitutes a new component of the endocytotic compartment, lack of boss internalisation into the R7 precursor cell is a direct consequence of hk mutations.

(Kramer et al., 1995)

Located between coordinates -85.2 and -82.5.

(Stathakis et al., 1995)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 40 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35169

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q24185

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q24185

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q24185

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001202

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0001202

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35169/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001202

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001202

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001202

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10653

Flygut - An atlas of the Drosophila adult midgut

FBgn0001202

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35169

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC004151

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG10653

MARRVEL_MODEL - MARRVEL (model organism gene)

35169

modMine - A data warehouse for the modENCODE project

FBgn0001202

Linkouts

BioGRID - A database of protein and genetic interactions.

61158

DroID - A comprehensive database of gene and protein interactions.

FBgn0001202

DRSC - Results frm RNAi screens

FBgn0001202

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0001202

FlyMine - An integrated database for Drosophila genomics

FBgn0001202

MIST (genetic) - An integrated Molecular Interaction Database

hook

MIST (protein-protein) - An integrated Molecular Interaction Database

hook

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG10653

(Hosono et al., 2015, Perkins et al., 2009)

Hook

(Gillingham et al., 2014, Liu et al., 2014)

dHk

(Walenta et al., 2001)

(Japanese National Institute of Genetics, 2012.5.21, Saja et al., 2010, Shao et al., 2010, Haberman et al., 2008, Cherry and Perrimon, 2004, McCrady and Tolin, 1994, Wright, 1987)

hook

(Lu et al., 2020, Hall et al., 2019, Jiang et al., 2019)

Secondary FlyBase IDs

Datasets (8)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (8)

Result

Result Type

Title

scRNAseq_2020_Cattenoz_NI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from non-infested third instar larvae

scRNAseq_2020_Cattenoz_WI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from wasp-infested third instar larvae

scRNAseq_2020_Cho_NI72LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying

scRNAseq_2020_Cho_NI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying

scRNAseq_2020_Cho_WI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI96HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying

References (122)

The following external sites may use different assemblies or annotations than FlyBase.

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