• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • RNA-Seq Search
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • GBrowse 2 Beta
    • CytoSearch
    • Feature Mapper
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Current release
  • Archived releases
  • Map Conversion
  • FTP site:
  • Releases (FTP)
  • Genomes (FTP)
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Vectors & Constructs
  • Other links:
  • BDGP
  • DGRC
  • DIS by issue
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\Hsc70-3

General Information
Symbol	Dmel\Hsc70-3	Species	D. melanogaster
Name	Heat shock 70-kDa protein cognate 3	Annotation symbol	CG4147
Feature type	protein_coding_gene	FlyBase ID	FBgn0001218
Gene Model Status	Current	Stock availability	27 publicly available
Also Known As	BiP, hsc3, Hsc70, Grp78, hsc72, l(1)G0102
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	10E3-10E4	Sequence location	X:11,695,729..11,701,150 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Heat shock 70-kDa protein cognate 3 is referred to in FlyBase by the symbol Dmel\Hsc70-3 (CG4147, FBgn0001218). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: ATPase activity. There is experimental evidence that it is involved in the biological process: RNA interference; centrosome duplication. 42 alleles are reported. The phenotypes of these alleles are annotated with: pupa; eye; rhabdomere R7; ommatidium; third instar larva. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Heat shock protein 70 family; Heat shock protein 70, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of high expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult testis. Comments on Affy2 ProbeSet: ProbeSet 1635423_s_at completely aligns to an exonic region common to each of the 4 FlyBase-annotated transcript isoforms of Hsc70-3. Gene sequence location is X:11695729..11701212.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 10E3-10E4   Limits computationally determined from genome sequence between P{EP}EP345&P{EP}EP1409 and P{EP}ATP7EP308
Experimentally Determined Cytological Location
Cytogenetic map Notes References 10E1-10E4   (determined by in situ hybridisation) 10E--F (determined by in situ hybridisation) 10E (determined by in situ hybridisation)   (Peter et al., 2002) 10E-10E   (determined by in situ hybridisation)   (Schaefer et al., 1999.12.2) 10E-10E   (determined by in situ hybridisation) 10E1--4 (determined by in situ hybridisation)   (Schaefer et al., 1999.8.12)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Hsc70-3 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.51 Annotated transcripts do not represent all supported alternative splices within 5' UTR. Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Hsc70-3-RA FBtr0073609  NM_167306   3019   656 Hsc70-3-RB FBtr0073608  NM_078577   3158   656 Hsc70-3-RC FBtr0073611  NM_167307   2985   656 Hsc70-3-RD FBtr0073610  NM_167308   3185   656
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Hsc70-3-PA   FBpp0073446   72.3   656   4.99     NP_727563   AAF48095 Hsc70-3-PB   FBpp0073445   72.3   656   4.99     NP_511132   AAN09299 Hsc70-3-PC   FBpp0073448   72.3   656   4.99     NP_727564   AAN09300 Hsc70-3-PD   FBpp0073447   72.3   656   4.99     NP_727565   AAN09301
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Heat shock protein 70, conserved site (IPR018181) Heat shock protein 70 family (IPR013126) MitoDrome - A database of annotated Dmel nuclear genes encoding mitochondrial proteins • MTDROME04147
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141334   AA246941   AA263545   AA439726   AA441117   AA539558   AA801499   AA948966   AA949040   AA949954   AA950808   AA951107   AE014298 AAF48095   AE014298 AAN09299   AE014298 AAN09300   AE014298 AAN09301   AI108095   AI258142   AI259584   AI260503   AI292896   AI294049   AI296142   AI297438   AI405700   AI513115   AI532193   AI533595   AI541986   AI542286   AI542481   AI543591   AJ426871   AJ426872   AJ426873   AJ426875   AJ426876   AJ569733   AJ569734   AJ569735   AJ569736   AJ569737   AJ569738   AJ569739   AJ569740   AJ569741   AJ569742   AJ569744   AJ569745   AJ569746   AJ569747   AJ569748   AJ569749   AJ569750   AJ569751   AJ569752   AJ569753   AJ569755   AJ569756   AJ569757   AJ569758   AK284625   AX153656   AY231692   AY432037   AY439522   BF485786   BF503444   BF505124   BG636939   BG637003   BG639383   BG639412   BG640155   BG640203   BG640971   BI162937   BI164720   BI167291   BI167807   BI168551   BI169116   BI169833   BI171533   BI172056   BI172577   BI172719   BI172839   BI212288   BI212296   BI212341   BI212660   BI213039   BI215458   BI230452   BI230474   BI231352   BI231359   BI232655   BI233302   BI234199   BI236277   BI236643   BI238064   BI240336   BI241591   BI241734   BI242446   BI243580   BI355600   BI356448   BI356531   BI357084   BI357967   BI362014   BI362852   BI366199   BI366768   BI367778   BI369009   BI371326   BI371558   BI373111   BI373805   BI374034   BI374268   BI481908   BI482380   BI482772   BI483268   BI483822   BI484000   BI485302   BI485923   BI567244   BI568582   BI577604   BI577860   BI578515   BI580060   BI580668   BI582973   BI617069   BI618537   BI625395   BI635343   BI636400   BI638177   BI639006   BP541689   BP546124   BQ103719   BT004838 AAO45194   BT126331   BX019691   BX034658   BX069833   CK131441   CK132239   CK445268   CK657810   CK658227   CK659279   CK660720   CL868484   CO153660   CO153822   CO180686   CO180731   CO182849   CO186075   CO188438   CO189170   CO195115   CO195178   CO195343   CO195528   CO265066   CO266067   CO267031   CO270790   CO274643   CO276206   CO277954   CO279701   CO284184   CO284969   CO286485   CO287240   CO288678   CO288700   CO298014   CO298069   CO299242   CO304583   CO306601   CO307967   CO308604   CO314158   CO317081   CO321540   CO323676   CO324041   CO325335   CO329335   CO329732   CO332425   CO335539   CZ472321   CZ472365   CZ473280   CZ473281   CZ473771   CZ474241   DQ440225   EC058695   EC063290   EC065431   EC070112   EC072935   EC074390   EC076803   EC080658   EC084386   EC085637   EC088268   EC197966   EC198183   EC200411   EC200913   EC204868   EC205290   EC211020   EC211776   EC216745   EC222575   EC226950   EC233472   EC234554   EC237583   EC238155   EC238235   EC239324   EC240679   EC241019   EC241240   EC242073   EC243242   EC244625   EC247607   EC261335   EC262722   ES695426   ES696327   ES696792   ES696809   EV587746   EV591737   EV593730   GH749838   GH750106   GH752694   GH754076   GH756874   GH757185   GH757317   GH757772   GH758973   GH761204   GH772000   GH772783   GH772792   GH773241   GH774969   GH780871   GH782226   GH782919   GH789068   GH790430   GH792743   GH793249   GH796110   GH800220   GH801118   GH803214   GH809793   GH809838   GH811286   GH812130   GH816727   GH819309   GH820224   GH820278   GH820750   GH822167   GH822235   GH824251   GH826813   GH829511   GH829594   GH835208   GH836770   GH837389   GH839630   GH840833   GH841375   GH843221   GH844854   GH847060   GH848920   GH853574   GH856535   GH857679   GH859780   GH859816   GH860157   GH860922   GH863349   GH864582   GH864787   GH865907   GH871515   GH873331   GH874975   GH875824   GH881780   GH884543   GH892721   GH894548   GH896212   GH898454   GH899544   GH902461   GH903145   GH903547   GH904777   GH904990   GH905784   GH907748   GH910539   GH913620   GH914647   GH914943   GH917531   GH919011   GH919248   GH920151   GH922277   GH923302   GH929148   GH929697   GH931705   GH932998   GH933754   GH934012   GH935394   GH937378   GH939902   GH941849   GH943257   GH944839   GH946499   GH952405   GH954009   GH954671   GH955323   GH955932   GH959179   GH959509   GH959625   GH960350   GH961965   GH962892   GH963519   GH963707   GH969322   GH972110   GH975194   GH976185   GH977706   GH980524   GH982344   GH982404   GH983123   GH984993   L01498 AAA28626   L01499
UniProtKB/Swiss-Prot	P29844
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference   cultured cell-line   • endoplasmic reticulum (Han et al., 2004) mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010) day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
	endoplasmic reticulum
Subcellular Localization (GO Cellular Component)
CV term Evidence References
endoplasmic reticulum inferred from direct assay (Ryoo et al., 2007) extracellular space inferred from direct assay (Walker et al., 2006) lipid particle inferred from direct assay (Beller et al., 2006, Beller et al., 2006, Cermelli et al., 2006) microtubule associated complex inferred from direct assay (Hughes et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1564.975 Larval Midgut    2183.4 Larval Hindgut    1917.1 Larval Malpighian Tubules    3428.1 Larval Fat Body    5449.1 Larval Salivary Gland    8181.1 Larval Trachea    1610 Larval Carcass    2966.325 Adult Head    1581 Adult Eye    1777.775 Adult Brain    1299.2 Adult Thoracic-Abdominal Ganglion    1593.6 Adult Crop    1837.9 Adult Midgut    1579.8 Adult Hindgut    1050.5 Adult Malpighian Tubules    3166.9 Adult Fat Body    2679.9 Adult Salivary Gland    3764.5 Adult Heart    2447.675 Adult VirginFemale Spermatheca    3531.9 Adult InseminatedFemale Spermatheca    3624.4 Adult Ovary    2070.2 Adult Testis    490.6 Adult Male Accessory Gland    6790.8 Adult Carcass    1625 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1564.975) Larval Midgut  (2183.4) Larval Hindgut  (1917.1) Larval Malpighian Tubules  (3428.1) Larval Fat Body  (5449.1) Larval Salivary Gland  (8181.1) Larval Trachea  (1610) Larval Carcass  (2966.325) Adult Head  (1581) Adult Eye  (1777.775) Adult Brain  (1299.2) Adult Thoracic-Abdominal Ganglion  (1593.6) Adult Crop  (1837.9) Adult Midgut  (1579.8) Adult Hindgut  (1050.5) Adult Malpighian Tubules  (3166.9) Adult Fat Body  (2679.9) Adult Salivary Gland  (3764.5) Adult Heart  (2447.675) Adult VirginFemale Spermatheca  (3531.9) Adult InseminatedFemale Spermatheca  (3624.4) Adult Ovary  (2070.2) Adult Testis    490.6 Adult Male Accessory Gland  (6790.8) Adult Carcass  (1625) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (1564.975) Larval Midgut  (2183.4) Larval Hindgut  (1917.1) Larval Malpighian Tubules  (3428.1) Larval Fat Body  (5449.1) Larval Salivary Gland  (8181.1) Larval Trachea  (1610) Larval Carcass  (2966.325) Adult Head  (1581) Adult Eye  (1777.775) Adult Brain  (1299.2) Adult Thoracic-Abdominal Ganglion  (1593.6) Adult Crop  (1837.9) Adult Midgut  (1579.8) Adult Hindgut  1050.5 Adult Malpighian Tubules  (3166.9) Adult Fat Body  (2679.9) Adult Salivary Gland  (3764.5) Adult Heart  (2447.675) Adult VirginFemale Spermatheca  (3531.9) Adult InseminatedFemale Spermatheca  (3624.4) Adult Ovary  (2070.2) Adult Testis    490.6 Adult Male Accessory Gland  (6790.8) Adult Carcass  (1625) Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1564.975 Larval Midgut    2183.4 Larval Hindgut    1917.1 Larval Malpighian Tubules    3428.1 Larval Fat Body    5449.1 Larval Salivary Gland    8181.1 Larval Trachea    1610 Larval Carcass    2966.325 Adult Head    1581 Adult Eye    1777.775 Adult Brain    1299.2 Adult Thoracic-Abdominal Ganglion    1593.6 Adult Crop    1837.9 Adult Midgut    1579.8 Adult Hindgut    1050.5 Adult Malpighian Tubules    3166.9 Adult Fat Body    2679.9 Adult Salivary Gland    3764.5 Adult Heart    2447.675 Adult VirginFemale Spermatheca    3531.9 Adult InseminatedFemale Spermatheca    3624.4 Adult Ovary    2070.2 Adult Testis    490.6 Adult Male Accessory Gland    6790.8 Adult Carcass    1625 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1564.975 Larval Midgut    2183.4 Larval Hindgut    1917.1 Larval Malpighian Tubules    3428.1 Larval Fat Body    5449.1 Larval Salivary Gland    8181.1 Larval Trachea    1610 Larval Carcass    2966.325 Adult Head    1581 Adult Eye    1777.775 Adult Brain    1299.2 Adult Thoracic-Abdominal Ganglion    1593.6 Adult Crop    1837.9 Adult Midgut    1579.8 Adult Hindgut    1050.5 Adult Malpighian Tubules    3166.9 Adult Fat Body    2679.9 Adult Salivary Gland    3764.5 Adult Heart    2447.675 Adult VirginFemale Spermatheca    3531.9 Adult InseminatedFemale Spermatheca    3624.4 Adult Ovary    2070.2 Adult Testis    490.6 Adult Male Accessory Gland    6790.8 Adult Carcass    1625 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1564.975) Larval Midgut  (2183.4) Larval Hindgut  (1917.1) Larval Malpighian Tubules  (3428.1) Larval Fat Body  (5449.1) Larval Salivary Gland  (8181.1) Larval Trachea  (1610) Larval Carcass  (2966.325) Adult Head  (1581) Adult Eye  (1777.775) Adult Brain  (1299.2) Adult Thoracic-Abdominal Ganglion  (1593.6) Adult Crop  (1837.9) Adult Midgut  (1579.8) Adult Hindgut  (1050.5) Adult Malpighian Tubules  (3166.9) Adult Fat Body  (2679.9) Adult Salivary Gland  (3764.5) Adult Heart  (2447.675) Adult VirginFemale Spermatheca  (3531.9) Adult InseminatedFemale Spermatheca  (3624.4) Adult Ovary  (2070.2) Adult Testis    490.6 Adult Male Accessory Gland  (6790.8) Adult Carcass  (1625) Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1564.975 Larval Midgut  (2183.4) Larval Hindgut  (1917.1) Larval Malpighian Tubules  (3428.1) Larval Fat Body  (5449.1) Larval Salivary Gland  (8181.1) Larval Trachea  1610 Larval Carcass  (2966.325) Adult Head  1581 Adult Eye  (1777.775) Adult Brain  1299.2 Adult Thoracic-Abdominal Ganglion  1593.6 Adult Crop  (1837.9) Adult Midgut  1579.8 Adult Hindgut  1050.5 Adult Malpighian Tubules  (3166.9) Adult Fat Body  (2679.9) Adult Salivary Gland  (3764.5) Adult Heart  (2447.675) Adult VirginFemale Spermatheca  (3531.9) Adult InseminatedFemale Spermatheca  (3624.4) Adult Ovary  (2070.2) Adult Testis    490.6 Adult Male Accessory Gland  (6790.8) Adult Carcass  1625 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1564.975 Larval Midgut    2183.4 Larval Hindgut    1917.1 Larval Malpighian Tubules    3428.1 Larval Fat Body    5449.1 Larval Salivary Gland    8181.1 Larval Trachea    1610 Larval Carcass    2966.325 Adult Head    1581 Adult Eye    1777.775 Adult Brain    1299.2 Adult Thoracic-Abdominal Ganglion    1593.6 Adult Crop    1837.9 Adult Midgut    1579.8 Adult Hindgut    1050.5 Adult Malpighian Tubules    3166.9 Adult Fat Body    2679.9 Adult Salivary Gland    3764.5 Adult Heart    2447.675 Adult VirginFemale Spermatheca    3531.9 Adult InseminatedFemale Spermatheca    3624.4 Adult Ovary    2070.2 Adult Testis    490.6 Adult Male Accessory Gland    6790.8 Adult Carcass    1625 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    1581   NA  Eye    1777.775   NA  Brain    1299.2   1564.975  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1593.6   NA  Crop    1837.9   2183.4  Midgut    1579.8   1917.1  Hindgut    1050.5   3428.1  Malpighian Tubules    3166.9   5449.1  Fat Body    2679.9   8181.1  Salivary Gland    3764.5   NA  Heart    2447.675   1610  Trachea    NA   NA  VirginFemale Spermatheca    3531.9   NA  InseminatedFemale Spermatheca    3624.4   NA  Ovary    2070.2   NA  Testis    490.6   NA  Male Accessory Gland    6790.8   2966.325  Carcass    1625 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    521 central nervous system, larvae L3    661 central nervous system, pupae P8    481 head, virgin 1-day female    421 head, virgin 4-day female    248 head, virgin 20-day female    195 head, mated 1-day female    259 head, mated 4-day female    288 head, mated 20-day female    204 head, mated 1-day male    218 head, mated 4-day male    332 head, mated 20-day male    374 salivary gland, larvae L3 wandering    547 salivary gland, white prepupae    3097 digestive system, larvae L3 wandering    478 digestive system, 1-day adult    409 digestive system, 4-day adult    594 digestive system, 20-day adult    358 fat body, larvae L3 wandering    1043 fat body, white prepupae    1065 fat body, pupae P8    1434 carcass, larvae L3 wandering    807 carcass, 1-day adult    315 carcass, 4-day adult    466 carcass, 20-day adult    330 ovary, virgin 4-day female    670 ovary, mated 4-day female    780 testis, mated 4-day male    213 accessory gland, mated 4-day male    1251 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (521) central nervous system, larvae L3  (661) central nervous system, pupae P8  (481) head, virgin 1-day female  (421) head, virgin 4-day female  (248) head, virgin 20-day female  (195) head, mated 1-day female  (259) head, mated 4-day female  (288) head, mated 20-day female  (204) head, mated 1-day male  (218) head, mated 4-day male  (332) head, mated 20-day male  (374) salivary gland, larvae L3 wandering  (547) salivary gland, white prepupae  (3097) digestive system, larvae L3 wandering  (478) digestive system, 1-day adult  (409) digestive system, 4-day adult  (594) digestive system, 20-day adult  (358) fat body, larvae L3 wandering  (1043) fat body, white prepupae  (1065) fat body, pupae P8  (1434) carcass, larvae L3 wandering  (807) carcass, 1-day adult  (315) carcass, 4-day adult  (466) carcass, 20-day adult  (330) ovary, virgin 4-day female  (670) ovary, mated 4-day female  (780) testis, mated 4-day male  (213) accessory gland, mated 4-day male  (1251) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (521) central nervous system, larvae L3  (661) central nervous system, pupae P8  (481) head, virgin 1-day female  (421) head, virgin 4-day female  (248) head, virgin 20-day female  (195) head, mated 1-day female  (259) head, mated 4-day female  (288) head, mated 20-day female  (204) head, mated 1-day male  (218) head, mated 4-day male  (332) head, mated 20-day male  (374) salivary gland, larvae L3 wandering  (547) salivary gland, white prepupae  (3097) digestive system, larvae L3 wandering  (478) digestive system, 1-day adult  (409) digestive system, 4-day adult  (594) digestive system, 20-day adult  (358) fat body, larvae L3 wandering  (1043) fat body, white prepupae  (1065) fat body, pupae P8  (1434) carcass, larvae L3 wandering  (807) carcass, 1-day adult  (315) carcass, 4-day adult  (466) carcass, 20-day adult  (330) ovary, virgin 4-day female  (670) ovary, mated 4-day female  (780) testis, mated 4-day male  (213) accessory gland, mated 4-day male  (1251) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    521 central nervous system, larvae L3    661 central nervous system, pupae P8    481 head, virgin 1-day female    421 head, virgin 4-day female    248 head, virgin 20-day female    195 head, mated 1-day female    259 head, mated 4-day female    288 head, mated 20-day female    204 head, mated 1-day male    218 head, mated 4-day male    332 head, mated 20-day male    374 salivary gland, larvae L3 wandering    547 salivary gland, white prepupae    3097 digestive system, larvae L3 wandering    478 digestive system, 1-day adult    409 digestive system, 4-day adult    594 digestive system, 20-day adult    358 fat body, larvae L3 wandering    1043 fat body, white prepupae    1065 fat body, pupae P8    1434 carcass, larvae L3 wandering    807 carcass, 1-day adult    315 carcass, 4-day adult    466 carcass, 20-day adult    330 ovary, virgin 4-day female    670 ovary, mated 4-day female    780 testis, mated 4-day male    213 accessory gland, mated 4-day male    1251 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    521 central nervous system, larvae L3    661 central nervous system, pupae P8    481 head, virgin 1-day female    421 head, virgin 4-day female    248 head, virgin 20-day female    195 head, mated 1-day female    259 head, mated 4-day female    288 head, mated 20-day female    204 head, mated 1-day male    218 head, mated 4-day male    332 head, mated 20-day male    374 salivary gland, larvae L3 wandering    547 salivary gland, white prepupae    3097 digestive system, larvae L3 wandering    478 digestive system, 1-day adult    409 digestive system, 4-day adult    594 digestive system, 20-day adult    358 fat body, larvae L3 wandering    1043 fat body, white prepupae    1065 fat body, pupae P8    1434 carcass, larvae L3 wandering    807 carcass, 1-day adult    315 carcass, 4-day adult    466 carcass, 20-day adult    330 ovary, virgin 4-day female    670 ovary, mated 4-day female    780 testis, mated 4-day male    213 accessory gland, mated 4-day male    1251 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (521) central nervous system, larvae L3  (661) central nervous system, pupae P8  (481) head, virgin 1-day female  (421) head, virgin 4-day female  (248) head, virgin 20-day female  (195) head, mated 1-day female  (259) head, mated 4-day female  (288) head, mated 20-day female  (204) head, mated 1-day male  (218) head, mated 4-day male  (332) head, mated 20-day male  (374) salivary gland, larvae L3 wandering  (547) salivary gland, white prepupae  (3097) digestive system, larvae L3 wandering  (478) digestive system, 1-day adult  (409) digestive system, 4-day adult  (594) digestive system, 20-day adult  (358) fat body, larvae L3 wandering  (1043) fat body, white prepupae  (1065) fat body, pupae P8  (1434) carcass, larvae L3 wandering  (807) carcass, 1-day adult  (315) carcass, 4-day adult  (466) carcass, 20-day adult  (330) ovary, virgin 4-day female  (670) ovary, mated 4-day female  (780) testis, mated 4-day male  (213) accessory gland, mated 4-day male  (1251) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (521) central nervous system, larvae L3  (661) central nervous system, pupae P8  (481) head, virgin 1-day female  (421) head, virgin 4-day female  (248) head, virgin 20-day female  (195) head, mated 1-day female  (259) head, mated 4-day female  (288) head, mated 20-day female  (204) head, mated 1-day male  (218) head, mated 4-day male  (332) head, mated 20-day male  (374) salivary gland, larvae L3 wandering  (547) salivary gland, white prepupae  (3097) digestive system, larvae L3 wandering  (478) digestive system, 1-day adult  (409) digestive system, 4-day adult  (594) digestive system, 20-day adult  (358) fat body, larvae L3 wandering  (1043) fat body, white prepupae  (1065) fat body, pupae P8  (1434) carcass, larvae L3 wandering  (807) carcass, 1-day adult  (315) carcass, 4-day adult  (466) carcass, 20-day adult  (330) ovary, virgin 4-day female  (670) ovary, mated 4-day female  (780) testis, mated 4-day male  (213) accessory gland, mated 4-day male  (1251) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    521 central nervous system, larvae L3    661 central nervous system, pupae P8    481 head, virgin 1-day female    421 head, virgin 4-day female    248 head, virgin 20-day female    195 head, mated 1-day female    259 head, mated 4-day female    288 head, mated 20-day female    204 head, mated 1-day male    218 head, mated 4-day male    332 head, mated 20-day male    374 salivary gland, larvae L3 wandering    547 salivary gland, white prepupae    3097 digestive system, larvae L3 wandering    478 digestive system, 1-day adult    409 digestive system, 4-day adult    594 digestive system, 20-day adult    358 fat body, larvae L3 wandering    1043 fat body, white prepupae    1065 fat body, pupae P8    1434 carcass, larvae L3 wandering    807 carcass, 1-day adult    315 carcass, 4-day adult    466 carcass, 20-day adult    330 ovary, virgin 4-day female    670 ovary, mated 4-day female    780 testis, mated 4-day male    213 accessory gland, mated 4-day male    1251 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of high expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    409 embryo 02-04hr    186 embryo 04-06hr    90 embryo 06-08hr    236 embryo 08-10hr    316 embryo 10-12hr    535 embryo 12-14hr    567 embryo 14-16hr    415 embryo 16-18hr    219 embryo 18-20hr    165 embryo 20-22hr    124 embryo 22-24hr    127 larva L1    291 larva L2    623 larva L3 12hr old    361 larva L3 puffstage 1-2    257 larva L3 puffstage 3-6    383 larva L3 puffstage 7-9    411 white prepupae new    585 white prepupae 12hr    231 white prepupae 24hr    439 pupae 2d postWPP    396 pupae 3d postWPP    127 pupae 4d postWPP    176 adult male 01day    272 adult male 05day    257 adult male 30day    299 adult female 01day    246 adult female 05day    340 adult female 30day    330 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (409) embryo 02-04hr  (186) embryo 04-06hr  (90) embryo 06-08hr  (236) embryo 08-10hr  (316) embryo 10-12hr  (535) embryo 12-14hr  (567) embryo 14-16hr  (415) embryo 16-18hr  (219) embryo 18-20hr  (165) embryo 20-22hr  (124) embryo 22-24hr  (127) larva L1  (291) larva L2  (623) larva L3 12hr old  (361) larva L3 puffstage 1-2  (257) larva L3 puffstage 3-6  (383) larva L3 puffstage 7-9  (411) white prepupae new  (585) white prepupae 12hr  (231) white prepupae 24hr  (439) pupae 2d postWPP  (396) pupae 3d postWPP  (127) pupae 4d postWPP  (176) adult male 01day  (272) adult male 05day  (257) adult male 30day  (299) adult female 01day  (246) adult female 05day  (340) adult female 30day  (330) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (409) embryo 02-04hr  (186) embryo 04-06hr    90 embryo 06-08hr  (236) embryo 08-10hr  (316) embryo 10-12hr  (535) embryo 12-14hr  (567) embryo 14-16hr  (415) embryo 16-18hr  (219) embryo 18-20hr  (165) embryo 20-22hr  (124) embryo 22-24hr  (127) larva L1  (291) larva L2  (623) larva L3 12hr old  (361) larva L3 puffstage 1-2  (257) larva L3 puffstage 3-6  (383) larva L3 puffstage 7-9  (411) white prepupae new  (585) white prepupae 12hr  (231) white prepupae 24hr  (439) pupae 2d postWPP  (396) pupae 3d postWPP  (127) pupae 4d postWPP  (176) adult male 01day  (272) adult male 05day  (257) adult male 30day  (299) adult female 01day  (246) adult female 05day  (340) adult female 30day  (330) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    409 embryo 02-04hr    186 embryo 04-06hr    90 embryo 06-08hr    236 embryo 08-10hr    316 embryo 10-12hr    535 embryo 12-14hr    567 embryo 14-16hr    415 embryo 16-18hr    219 embryo 18-20hr    165 embryo 20-22hr    124 embryo 22-24hr    127 larva L1    291 larva L2    623 larva L3 12hr old    361 larva L3 puffstage 1-2    257 larva L3 puffstage 3-6    383 larva L3 puffstage 7-9    411 white prepupae new    585 white prepupae 12hr    231 white prepupae 24hr    439 pupae 2d postWPP    396 pupae 3d postWPP    127 pupae 4d postWPP    176 adult male 01day    272 adult male 05day    257 adult male 30day    299 adult female 01day    246 adult female 05day    340 adult female 30day    330 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    409 embryo 02-04hr    186 embryo 04-06hr    90 embryo 06-08hr    236 embryo 08-10hr    316 embryo 10-12hr    535 embryo 12-14hr    567 embryo 14-16hr    415 embryo 16-18hr    219 embryo 18-20hr    165 embryo 20-22hr    124 embryo 22-24hr    127 larva L1    291 larva L2    623 larva L3 12hr old    361 larva L3 puffstage 1-2    257 larva L3 puffstage 3-6    383 larva L3 puffstage 7-9    411 white prepupae new    585 white prepupae 12hr    231 white prepupae 24hr    439 pupae 2d postWPP    396 pupae 3d postWPP    127 pupae 4d postWPP    176 adult male 01day    272 adult male 05day    257 adult male 30day    299 adult female 01day    246 adult female 05day    340 adult female 30day    330 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (409) embryo 02-04hr  (186) embryo 04-06hr  (90) embryo 06-08hr  (236) embryo 08-10hr  (316) embryo 10-12hr  (535) embryo 12-14hr  (567) embryo 14-16hr  (415) embryo 16-18hr  (219) embryo 18-20hr  (165) embryo 20-22hr  (124) embryo 22-24hr  (127) larva L1  (291) larva L2  (623) larva L3 12hr old  (361) larva L3 puffstage 1-2  (257) larva L3 puffstage 3-6  (383) larva L3 puffstage 7-9  (411) white prepupae new  (585) white prepupae 12hr  (231) white prepupae 24hr  (439) pupae 2d postWPP  (396) pupae 3d postWPP  (127) pupae 4d postWPP  (176) adult male 01day  (272) adult male 05day  (257) adult male 30day  (299) adult female 01day  (246) adult female 05day  (340) adult female 30day  (330) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (409) embryo 02-04hr  (186) embryo 04-06hr    90 embryo 06-08hr  (236) embryo 08-10hr  (316) embryo 10-12hr  (535) embryo 12-14hr  (567) embryo 14-16hr  (415) embryo 16-18hr  (219) embryo 18-20hr  (165) embryo 20-22hr  124 embryo 22-24hr  127 larva L1  (291) larva L2  (623) larva L3 12hr old  (361) larva L3 puffstage 1-2  (257) larva L3 puffstage 3-6  (383) larva L3 puffstage 7-9  (411) white prepupae new  (585) white prepupae 12hr  (231) white prepupae 24hr  (439) pupae 2d postWPP  (396) pupae 3d postWPP  127 pupae 4d postWPP  (176) adult male 01day  (272) adult male 05day  (257) adult male 30day  (299) adult female 01day  (246) adult female 05day  (340) adult female 30day  (330) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    409 embryo 02-04hr    186 embryo 04-06hr    90 embryo 06-08hr    236 embryo 08-10hr    316 embryo 10-12hr    535 embryo 12-14hr    567 embryo 14-16hr    415 embryo 16-18hr    219 embryo 18-20hr    165 embryo 20-22hr    124 embryo 22-24hr    127 larva L1    291 larva L2    623 larva L3 12hr old    361 larva L3 puffstage 1-2    257 larva L3 puffstage 3-6    383 larva L3 puffstage 7-9    411 white prepupae new    585 white prepupae 12hr    231 white prepupae 24hr    439 pupae 2d postWPP    396 pupae 3d postWPP    127 pupae 4d postWPP    176 adult male 01day    272 adult male 05day    257 adult male 30day    299 adult female 01day    246 adult female 05day    340 adult female 30day    330 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    948 Schneider line 2 Sg4    805 embryonic 1182-4H    1016 embryonic GM2    925 embryonic Kc167    891 embryonic S1    431 embryonic S3    2532 leg disc CME L1    1442 wing disc CME-W2    681 wing disc ML-DmD8    513 wing disc ML-DmD9    729 wing disc ML-DmD16-c3    792 wing disc ML-DmD21    394 wing disc ML-DmD32    1081 haltere disc ML-DmD17-c3    345 eye-antennal disc ML-DmD11    617 antennal disc ML-DmD20-c5    773 mixed discs ML-DmD4-c1    873 CNS ML-DmBG1-c1    1083 CNS ML-DmBG2-c2    869 tumorous blood cells mbn2    1182 ovary fGS/OSS    618 ovary OSC    416 ovary OSS    388 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (948) Schneider line 2 Sg4  (805) embryonic 1182-4H  (1016) embryonic GM2  (925) embryonic Kc167  (891) embryonic S1  (431) embryonic S3  (2532) leg disc CME L1  (1442) wing disc CME-W2  (681) wing disc ML-DmD8  (513) wing disc ML-DmD9  (729) wing disc ML-DmD16-c3  (792) wing disc ML-DmD21  (394) wing disc ML-DmD32  (1081) haltere disc ML-DmD17-c3  (345) eye-antennal disc ML-DmD11  (617) antennal disc ML-DmD20-c5  (773) mixed discs ML-DmD4-c1  (873) CNS ML-DmBG1-c1  (1083) CNS ML-DmBG2-c2  (869) tumorous blood cells mbn2  (1182) ovary fGS/OSS  (618) ovary OSC  (416) ovary OSS  (388) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (948) Schneider line 2 Sg4  (805) embryonic 1182-4H  (1016) embryonic GM2  (925) embryonic Kc167  (891) embryonic S1  (431) embryonic S3  (2532) leg disc CME L1  (1442) wing disc CME-W2  (681) wing disc ML-DmD8  (513) wing disc ML-DmD9  (729) wing disc ML-DmD16-c3  (792) wing disc ML-DmD21  (394) wing disc ML-DmD32  (1081) haltere disc ML-DmD17-c3  (345) eye-antennal disc ML-DmD11  (617) antennal disc ML-DmD20-c5  (773) mixed discs ML-DmD4-c1  (873) CNS ML-DmBG1-c1  (1083) CNS ML-DmBG2-c2  (869) tumorous blood cells mbn2  (1182) ovary fGS/OSS  (618) ovary OSC  (416) ovary OSS  (388) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    948 Schneider line 2 Sg4    805 embryonic 1182-4H    1016 embryonic GM2    925 embryonic Kc167    891 embryonic S1    431 embryonic S3    2532 leg disc CME L1    1442 wing disc CME-W2    681 wing disc ML-DmD8    513 wing disc ML-DmD9    729 wing disc ML-DmD16-c3    792 wing disc ML-DmD21    394 wing disc ML-DmD32    1081 haltere disc ML-DmD17-c3    345 eye-antennal disc ML-DmD11    617 antennal disc ML-DmD20-c5    773 mixed discs ML-DmD4-c1    873 CNS ML-DmBG1-c1    1083 CNS ML-DmBG2-c2    869 tumorous blood cells mbn2    1182 ovary fGS/OSS    618 ovary OSC    416 ovary OSS    388 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    948 Schneider line 2 Sg4    805 embryonic 1182-4H    1016 embryonic GM2    925 embryonic Kc167    891 embryonic S1    431 embryonic S3    2532 leg disc CME L1    1442 wing disc CME-W2    681 wing disc ML-DmD8    513 wing disc ML-DmD9    729 wing disc ML-DmD16-c3    792 wing disc ML-DmD21    394 wing disc ML-DmD32    1081 haltere disc ML-DmD17-c3    345 eye-antennal disc ML-DmD11    617 antennal disc ML-DmD20-c5    773 mixed discs ML-DmD4-c1    873 CNS ML-DmBG1-c1    1083 CNS ML-DmBG2-c2    869 tumorous blood cells mbn2    1182 ovary fGS/OSS    618 ovary OSC    416 ovary OSS    388 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (948) Schneider line 2 Sg4  (805) embryonic 1182-4H  (1016) embryonic GM2  (925) embryonic Kc167  (891) embryonic S1  (431) embryonic S3  (2532) leg disc CME L1  (1442) wing disc CME-W2  (681) wing disc ML-DmD8  (513) wing disc ML-DmD9  (729) wing disc ML-DmD16-c3  (792) wing disc ML-DmD21  (394) wing disc ML-DmD32  (1081) haltere disc ML-DmD17-c3  (345) eye-antennal disc ML-DmD11  (617) antennal disc ML-DmD20-c5  (773) mixed discs ML-DmD4-c1  (873) CNS ML-DmBG1-c1  (1083) CNS ML-DmBG2-c2  (869) tumorous blood cells mbn2  (1182) ovary fGS/OSS  (618) ovary OSC  (416) ovary OSS  (388) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (948) Schneider line 2 Sg4  (805) embryonic 1182-4H  (1016) embryonic GM2  (925) embryonic Kc167  (891) embryonic S1  (431) embryonic S3  (2532) leg disc CME L1  (1442) wing disc CME-W2  (681) wing disc ML-DmD8  (513) wing disc ML-DmD9  (729) wing disc ML-DmD16-c3  (792) wing disc ML-DmD21  (394) wing disc ML-DmD32  (1081) haltere disc ML-DmD17-c3  (345) eye-antennal disc ML-DmD11  (617) antennal disc ML-DmD20-c5  (773) mixed discs ML-DmD4-c1  (873) CNS ML-DmBG1-c1  (1083) CNS ML-DmBG2-c2  (869) tumorous blood cells mbn2  (1182) ovary fGS/OSS  (618) ovary OSC  (416) ovary OSS  (388) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    948 Schneider line 2 Sg4    805 embryonic 1182-4H    1016 embryonic GM2    925 embryonic Kc167    891 embryonic S1    431 embryonic S3    2532 leg disc CME L1    1442 wing disc CME-W2    681 wing disc ML-DmD8    513 wing disc ML-DmD9    729 wing disc ML-DmD16-c3    792 wing disc ML-DmD21    394 wing disc ML-DmD32    1081 haltere disc ML-DmD17-c3    345 eye-antennal disc ML-DmD11    617 antennal disc ML-DmD20-c5    773 mixed discs ML-DmD4-c1    873 CNS ML-DmBG1-c1    1083 CNS ML-DmBG2-c2    869 tumorous blood cells mbn2    1182 ovary fGS/OSS    618 ovary OSC    416 ovary OSS    388 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    866 cold shock, 4-day adult    743 heat shock, 4-day adult    656 Cadmium 50 mM 6 hrs, larvae L3    447 Cadmium 50 mM 12 hrs, larvae L3    372 Cadmium 50 mM 48 hrs, 4-day adult    675 Cadmium 100 mM 48 hrs, 4-day adult    1362 Copper 0.5 mM 12 hrs, larvae L3    1222 Copper 15 mM 48 hrs, 4-day adult    518 Zinc 5 mM 12 hrs, larvae L3    1049 Zinc 4.5 mM 48 hrs, 4-day adult    462 Ethanol 2.5% 3 hrs, larvae L3    812 Ethanol 5% 3 hrs, larvae L3    347 Ethanol 10% 3 hrs, larvae L3    450 Caffeine 1.5 mg/ml 4 hrs, larvae L3    209 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    578 Caffeine 25 mg/ml 48 hrs, 4-day adult    656 Paraquat 5 mM 48 hrs, 4-day adult    442 Paraquat 10 mM 48 hrs, 4-day adult    357 Rotenone 2 μg 12 hrs, larvae L3    494 Rotenone 8 μg 12 hrs, larvae L3    396 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (866) cold shock, 4-day adult  (743) heat shock, 4-day adult  (656) Cadmium 50 mM 6 hrs, larvae L3  (447) Cadmium 50 mM 12 hrs, larvae L3  (372) Cadmium 50 mM 48 hrs, 4-day adult  (675) Cadmium 100 mM 48 hrs, 4-day adult  (1362) Copper 0.5 mM 12 hrs, larvae L3  (1222) Copper 15 mM 48 hrs, 4-day adult  (518) Zinc 5 mM 12 hrs, larvae L3  (1049) Zinc 4.5 mM 48 hrs, 4-day adult  (462) Ethanol 2.5% 3 hrs, larvae L3  (812) Ethanol 5% 3 hrs, larvae L3  (347) Ethanol 10% 3 hrs, larvae L3  (450) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (209) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (578) Caffeine 25 mg/ml 48 hrs, 4-day adult  (656) Paraquat 5 mM 48 hrs, 4-day adult  (442) Paraquat 10 mM 48 hrs, 4-day adult  (357) Rotenone 2 μg 12 hrs, larvae L3  (494) Rotenone 8 μg 12 hrs, larvae L3  (396) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (866) cold shock, 4-day adult  (743) heat shock, 4-day adult  (656) Cadmium 50 mM 6 hrs, larvae L3  (447) Cadmium 50 mM 12 hrs, larvae L3  (372) Cadmium 50 mM 48 hrs, 4-day adult  (675) Cadmium 100 mM 48 hrs, 4-day adult  (1362) Copper 0.5 mM 12 hrs, larvae L3  (1222) Copper 15 mM 48 hrs, 4-day adult  (518) Zinc 5 mM 12 hrs, larvae L3  (1049) Zinc 4.5 mM 48 hrs, 4-day adult  (462) Ethanol 2.5% 3 hrs, larvae L3  (812) Ethanol 5% 3 hrs, larvae L3  (347) Ethanol 10% 3 hrs, larvae L3  (450) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (209) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (578) Caffeine 25 mg/ml 48 hrs, 4-day adult  (656) Paraquat 5 mM 48 hrs, 4-day adult  (442) Paraquat 10 mM 48 hrs, 4-day adult  (357) Rotenone 2 μg 12 hrs, larvae L3  (494) Rotenone 8 μg 12 hrs, larvae L3  (396) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    866 cold shock, 4-day adult    743 heat shock, 4-day adult    656 Cadmium 50 mM 6 hrs, larvae L3    447 Cadmium 50 mM 12 hrs, larvae L3    372 Cadmium 50 mM 48 hrs, 4-day adult    675 Cadmium 100 mM 48 hrs, 4-day adult    1362 Copper 0.5 mM 12 hrs, larvae L3    1222 Copper 15 mM 48 hrs, 4-day adult    518 Zinc 5 mM 12 hrs, larvae L3    1049 Zinc 4.5 mM 48 hrs, 4-day adult    462 Ethanol 2.5% 3 hrs, larvae L3    812 Ethanol 5% 3 hrs, larvae L3    347 Ethanol 10% 3 hrs, larvae L3    450 Caffeine 1.5 mg/ml 4 hrs, larvae L3    209 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    578 Caffeine 25 mg/ml 48 hrs, 4-day adult    656 Paraquat 5 mM 48 hrs, 4-day adult    442 Paraquat 10 mM 48 hrs, 4-day adult    357 Rotenone 2 μg 12 hrs, larvae L3    494 Rotenone 8 μg 12 hrs, larvae L3    396 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    866 cold shock, 4-day adult    743 heat shock, 4-day adult    656 Cadmium 50 mM 6 hrs, larvae L3    447 Cadmium 50 mM 12 hrs, larvae L3    372 Cadmium 50 mM 48 hrs, 4-day adult    675 Cadmium 100 mM 48 hrs, 4-day adult    1362 Copper 0.5 mM 12 hrs, larvae L3    1222 Copper 15 mM 48 hrs, 4-day adult    518 Zinc 5 mM 12 hrs, larvae L3    1049 Zinc 4.5 mM 48 hrs, 4-day adult    462 Ethanol 2.5% 3 hrs, larvae L3    812 Ethanol 5% 3 hrs, larvae L3    347 Ethanol 10% 3 hrs, larvae L3    450 Caffeine 1.5 mg/ml 4 hrs, larvae L3    209 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    578 Caffeine 25 mg/ml 48 hrs, 4-day adult    656 Paraquat 5 mM 48 hrs, 4-day adult    442 Paraquat 10 mM 48 hrs, 4-day adult    357 Rotenone 2 μg 12 hrs, larvae L3    494 Rotenone 8 μg 12 hrs, larvae L3    396 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (866) cold shock, 4-day adult  (743) heat shock, 4-day adult  (656) Cadmium 50 mM 6 hrs, larvae L3  (447) Cadmium 50 mM 12 hrs, larvae L3  (372) Cadmium 50 mM 48 hrs, 4-day adult  (675) Cadmium 100 mM 48 hrs, 4-day adult  (1362) Copper 0.5 mM 12 hrs, larvae L3  (1222) Copper 15 mM 48 hrs, 4-day adult  (518) Zinc 5 mM 12 hrs, larvae L3  (1049) Zinc 4.5 mM 48 hrs, 4-day adult  (462) Ethanol 2.5% 3 hrs, larvae L3  (812) Ethanol 5% 3 hrs, larvae L3  (347) Ethanol 10% 3 hrs, larvae L3  (450) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (209) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (578) Caffeine 25 mg/ml 48 hrs, 4-day adult  (656) Paraquat 5 mM 48 hrs, 4-day adult  (442) Paraquat 10 mM 48 hrs, 4-day adult  (357) Rotenone 2 μg 12 hrs, larvae L3  (494) Rotenone 8 μg 12 hrs, larvae L3  (396) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (866) cold shock, 4-day adult  (743) heat shock, 4-day adult  (656) Cadmium 50 mM 6 hrs, larvae L3  (447) Cadmium 50 mM 12 hrs, larvae L3  (372) Cadmium 50 mM 48 hrs, 4-day adult  (675) Cadmium 100 mM 48 hrs, 4-day adult  (1362) Copper 0.5 mM 12 hrs, larvae L3  (1222) Copper 15 mM 48 hrs, 4-day adult  (518) Zinc 5 mM 12 hrs, larvae L3  (1049) Zinc 4.5 mM 48 hrs, 4-day adult  (462) Ethanol 2.5% 3 hrs, larvae L3  (812) Ethanol 5% 3 hrs, larvae L3  (347) Ethanol 10% 3 hrs, larvae L3  (450) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (209) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (578) Caffeine 25 mg/ml 48 hrs, 4-day adult  (656) Paraquat 5 mM 48 hrs, 4-day adult  (442) Paraquat 10 mM 48 hrs, 4-day adult  (357) Rotenone 2 μg 12 hrs, larvae L3  (494) Rotenone 8 μg 12 hrs, larvae L3  (396) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    866 cold shock, 4-day adult    743 heat shock, 4-day adult    656 Cadmium 50 mM 6 hrs, larvae L3    447 Cadmium 50 mM 12 hrs, larvae L3    372 Cadmium 50 mM 48 hrs, 4-day adult    675 Cadmium 100 mM 48 hrs, 4-day adult    1362 Copper 0.5 mM 12 hrs, larvae L3    1222 Copper 15 mM 48 hrs, 4-day adult    518 Zinc 5 mM 12 hrs, larvae L3    1049 Zinc 4.5 mM 48 hrs, 4-day adult    462 Ethanol 2.5% 3 hrs, larvae L3    812 Ethanol 5% 3 hrs, larvae L3    347 Ethanol 10% 3 hrs, larvae L3    450 Caffeine 1.5 mg/ml 4 hrs, larvae L3    209 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    578 Caffeine 25 mg/ml 48 hrs, 4-day adult    656 Paraquat 5 mM 48 hrs, 4-day adult    442 Paraquat 10 mM 48 hrs, 4-day adult    357 Rotenone 2 μg 12 hrs, larvae L3    494 Rotenone 8 μg 12 hrs, larvae L3    396 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_03 mE1_20_mRNA_expression_cluster_10
External Data & Images
Linkouts	BDGP expression data • RH21402 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001218 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4147-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4how-24B Hsc70-3D231S.Scer\UAS Hsc70-3K97S.Scer\UAS lethal, with Scer\GAL4pnr-MD237 Hsc70-3GD6484 lethal | dominant, with Scer\GAL4unspecified Hsc70-3K97S.Scer\UAS lethal | embryonic stage Hsc70-3G0341 lethal | larval stage Hsc70-3G0102 Hsc70-3G0111 Hsc70-3G0292 Hsc70-3G0407 Hsc70-3G0466b lethal | larval stage, with Scer\GAL4337Y Hsc70-3D231S.Scer\UAS Hsc70-3K97S.Scer\UAS lethal | pharate adult stage Hsc70-3G0407 lethal | prepupal stage Hsc70-3G0407 lethal | pupal stage Hsc70-3G0407 lethal | recessive Hsc70-3G0102 Hsc70-3G0111 Hsc70-3G0292 Hsc70-3G0341 Hsc70-3G0466b Hsc70-3PG6 Hsc70-3PG34 Hsc70-3PG39 Hsc70-3PG65 Hsc70-3PG84 Hsc70-3PG86 Hsc70-3PG119 Hsc70-3PL11 Hsc70-3PL68 Hsc70-3PL73 partially lethal - majority die | recessive Hsc70-3G0407 viable Hsc70-3EY11233 viable, with Scer\GAL4how-24B Hsc70-3Scer\UAS.cEa Sterility Allele fertile Hsc70-3EY11233 Other Phenotypes Allele behavior defective, with Scer\GAL4156Y Hsc70-3D231S.Scer\UAS Phenotype manifest in Allele eye, with Scer\GAL4GMR.PF Hsc70-3K97S.Scer\UAS ommatidium, with Scer\GAL4GMR.PF Hsc70-3K97S.Scer\UAS pupa Hsc70-3G0407 rhabdomere R7, with Scer\GAL4Rh3.PP Hsc70-3GS1150 third instar larva Hsc70-3G0466b
Classical Alleles ( 34 )
For All Classical Alleles Show
Allele of Hsc70-3 Class Mutagen Stocks Known lesion Hsc70-3G0102 P-element activity 2 -- Hsc70-3G0111 P-element activity 2 -- Hsc70-35-SZ-3645 1 -- Hsc70-3CB-5201-3 1 -- Hsc70-3CB-5825-3 1 -- Hsc70-3d01593 P-element activity 1 -- Hsc70-3EY11233 P-element activity 1 -- Hsc70-3f04060 1 -- Hsc70-3G0292 P-element activity 1 -- Hsc70-3G0341 P-element activity 1 -- Hsc70-3G0407 P-element activity 1 -- Hsc70-3GS1150 1 Yes Hsc70-3NP6046 1 -- Hsc70-3NP6055 1 -- Hsc70-3NP7229 1 -- Hsc70-3d01672 P-element activity 0 -- Hsc70-3d03307 P-element activity 0 -- Hsc70-3d04173 P-element activity 0 -- Hsc70-3d04969 P-element activity 0 -- Hsc70-3EP1507 0 -- Hsc70-3EP336b P-element activity 0 -- Hsc70-3G0466b P-element activity 0 -- Hsc70-3GG01011 0 -- Hsc70-3KG01642 0 -- Hsc70-3PG119 Delta2-3 0 -- Hsc70-3PG34 Delta2-3 0 -- Hsc70-3PG39 Delta2-3 0 -- Hsc70-3PG65 Delta2-3 0 -- Hsc70-3PG6 Delta2-3 0 -- Hsc70-3PG84 Delta2-3 0 -- Hsc70-3PG86 Delta2-3 0 -- Hsc70-3PL11 Delta2-3 0 -- Hsc70-3PL68 Delta2-3 0 -- Hsc70-3PL73 Delta2-3 0 --
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Hsc70-3 Class Mutagen Stocks Known lesion Hsc70-3D231S.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 2 Yes Hsc70-3GD6484 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Hsc70-3K97S.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 2 Yes Hsc70-3Scer\UAS.cEa in vitro construct - regulatory fusion 2 Yes Hsc70-3HMS00397 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hsc70-3KK109452 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hsc70-3dsRNA.cDa in vitro construct - RNAi 0 Yes Hsc70-3dsRNA.cDb in vitro construct - RNAi 0 Yes
Aneuploid Aberrations
Duplicated in	Dp(1;Y)BSC1 (Gornek et al., 2000.2.4, Cook et al., 2010) Dp(1;Y)BSC2 (Gornek et al., 2000.2.4, Cook et al., 2010)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD6484} NA P{KK109452} NA P{TRiP.HMS00397} NA P{UAS-Hsc70-3.D231S} NA P{UAS-Hsc70-3.K97S} NA P{UAS-Hsc70-3.WT} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}Hsc70-3EP336b NA P{EP}Hsc70-3EP1507 NA P{EPgy2}Hsc70-3EY11233 NA P{GSV1}Hsc70-3GS1150 NA P{Mae-UAS.6.11}Hsc70-3GG01011 NA P{XP}Hsc70-3d01593 NA P{XP}Hsc70-3d01672 NA P{XP}Hsc70-3d03307 NA P{XP}Hsc70-3d04173 NA P{XP}Hsc70-3d04969 NA miscellaneous insertions P{RS3}Hsc70-3CB-5201-3 NA P{RS3}Hsc70-3CB-5825-3 NA P{RS5}Hsc70-35-SZ-3645 NA P{SUPor-P}Hsc70-3KG01642 NA PBac{WH}Hsc70-3f04060 NA insertion of enhancer trap binary system P{GawB}Hsc70-3NP6046 No P{GawB}Hsc70-3NP6055 No P{GawB}Hsc70-3NP7229 No P{GawB}Hsc70-3PG6 No P{GawB}Hsc70-3PG34 No P{GawB}Hsc70-3PG39 No P{GawB}Hsc70-3PG65 No P{GawB}Hsc70-3PG84 No P{GawB}Hsc70-3PG86 No P{GawB}Hsc70-3PG119 No insertion of enhancer trap P{lacW}Hsc70-3G0102 No P{lacW}Hsc70-3G0111 No P{lacW}Hsc70-3G0292 No P{lacW}Hsc70-3G0341 No P{lacW}Hsc70-3G0407 No P{lacW}Hsc70-3G0466b No P{lacW}Hsc70-3PL11 No P{lacW}Hsc70-3PL68 No P{lacW}Hsc70-3PL73 No P{lacW}l(1)G0175G0175 No
Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
centrosome duplication inferred from mutant phenotype (Müller et al., 2010) RNA interference inferred from mutant phenotype (Dorner et al., 2006)
Cellular Component
CV term Evidence References
endoplasmic reticulum inferred from direct assay (Ryoo et al., 2007) extracellular space inferred from direct assay (Walker et al., 2006) lipid particle inferred from direct assay (Beller et al., 2006, Beller et al., 2006, Cermelli et al., 2006) microtubule associated complex inferred from direct assay (Hughes et al., 2008)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
ATPase activity non-traceable author statement (Rubin, 1992)
Biological Process
CV term Evidence References
response to heat inferred from sequence or structural similarity with UniProtKB:Q90593 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1993.4.1) sleep traceable author statement (Cirelli, 2003)
Cellular Component
CV term Evidence References
endoplasmic reticulum non-traceable author statement (Cirelli, 2003, Swiss-Prot Project Members, 1993.4.1)
Sequence Ontology: Class of Gene
	gene_with_edited_transcript (Graveley et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Hsc70-3 - CG12691 experimental knowledge based (Guruharsha et al., 2011) Hsc70-3 - CG17343 experimental knowledge based (Guruharsha et al., 2011) Hsc70-3 - CG4933 experimental knowledge based (Guruharsha et al., 2011) Hsc70-3 - CG7737 experimental knowledge based (Guruharsha et al., 2011) Hsc70-3 - Dredd anti tag coimmunoprecipitation, peptide massfingerprinting (Fukuyama et al., 2012) Hsc70-3 - Gαo pull down, peptide massfingerprinting (Katanayeva et al., 2010) Hsc70-3 - Synd experimental knowledge based (Guruharsha et al., 2011) Hsc70-3 - hbs experimental knowledge based (Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Hsc70-3 allele Gene References unspecified Ppt1 (Buff et al., 2007)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 58539 DroID - A comprehensive database of gene and protein interactions. • FBgn0001218 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32133
Orthologs
OrthoDB Orthologs (39) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6GQQQJ Dipteran inclusive ortholog search EOG6Z8Z4N Insect inclusive ortholog search EOG6PK13Z Arthropod inclusive ortholog search EOG6ZCRKK Metazoa inclusive ortholog search EOG65N0Z9
Orthologs in Drosophila Species (EOG6GQQQJ)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Hsc70-3     Drosophila simulans   Dsim\GD15970 Y   Drosophila sechellia   Dsec\GM13044 Y   Drosophila erecta   Dere\GG18832     Drosophila yakuba   Dyak\Hsc70-3 Y   Drosophila ananassae   Dana\GF20248 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA17988 Y   Drosophila persimilis   Dper\GL20354 Y   Drosophila willistoni   Dwil\GK18328 Y   Drosophila virilis   Dvir\GJ16781 Y   Drosophila mojavensis   Dmoj\GI14717 Y   Drosophila grimshawi   Dgri\GH11975 Y
Orthologs in non-Drosophila Dipterans (EOG6Z8Z4N)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL017349   Anopheles gambiae Malaria mosquito  Agam\AGAP004192   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ003550
Orthologs in non-Dipteran Insects (EOG6PK13Z)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB49117   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG005290   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH10173   Atta cephalotes Leafcutter ant  Acep\ACEP20720   Camponotus floridanus Florida carpenter ant  Cflo\CFLO15163   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL12505   Linepithema humile Argentine ant  Lhum\LH23404   Pogonomyrmex barbatus Red harvester ant  Pbar\PB15578   Solenopsis invicta Red fire ant  Sinv\SINV18127   Acyrthosiphon pisum Pea aphid  Apim\ACYPI007166   Bombyx mori Silkmoth  Bmor\BGIBMGA007950   Pediculus humanus Human body louse  Phum\PHUM490960   Tribolium castaneum Red flour beetle  Tcas\TC004425
Orthologs in non-Insect Arthropods (EOG6ZCRKK)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Med14   Ixodes scapularis Deer tick  Isca\ISCW017754
Orthologs in non-Arthropod Metazoa (EOG65N0Z9)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\hsp-3   Caenorhabditis elegans Nematode  Cele\hsp-4   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_008560gn   Danio rerio Zebrafish  Drer\hspa5   Xenopus tropicalis Western clawed frog  Xtro\hspa5   Gallus gallus Domestic chicken  Ggal\HSPA5   Mus musculus House mouse  Mmus\Hspa5   Rattus norvegicus Norway rat  Rnor\Hspa5   Homo sapiens Human  Hsap\HSPA5
Human Orthologs (1)
Gene OMIM HGNC Hsap\HSPA5
AAA Orthologs (10) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD15970 Drosophila sechellia Dsec\GM13044 Drosophila yakuba Dyak\Hsc70-3 Drosophila ananassae Dana\GF20248 Drosophila pseudoobscura pseudoobscura Dpse\GA17988 Drosophila persimilis Dper\GL20354 Drosophila willistoni Dwil\GK18328 Drosophila virilis Dvir\GJ16781 Drosophila mojavensis Dmoj\GI14717 Drosophila grimshawi Dgri\GH11975
Stocks & Reagents
Stocks Listed in FlyBase ( 27 )
Bloomington	5843 w126; P{UAS-Hsc70-3.WT}B 5842 w126; P{UAS-Hsc70-3.K97S}D 5841 w126; P{UAS-Hsc70-3.D231S}D
Harvard	- P{XP}Hsc70-3d01593 - PBac{WH}Hsc70-3f04060
Kyoto	108461 w126; P{UAS-Hsc70-3.WT}B 108460 w126; P{UAS-Hsc70-3.K97S}D 108459 w126; P{UAS-Hsc70-3.D231S}D 123507 w1118 P{RS3}Hsc70-3CB-5201-3
VDRC	v101766 P{KK109452}VIE-260B v14882 w1118; P{GD6484}v14882 v14883 w1118; P{GD6484}v14883
	More stocks available...
Genomic Clones ( 1 )
	BACR46E23 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 334 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD03228 RH21402
Other clones	yak-em_Hsc70
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT07291 AT19434 AT19833 GH01705 GH06832 GH15922 GH25812 GM31542 HL02648 LD05950 LD06656 LD14365 LD16010 LD17746 LD27682 LD27782 LD28532 LD30943 LD31403 LP01263 LP03033 LP03836 LP04391 LP07256 LP07341 LP09211 LP09966 LP11736 LP13614 LP14282 LP14995 LP16932 LP19540 LP24392 RE02218 RE04452 RE07641 RE08279 RE09322 RE10094 RE11119 RE13335 RE13982 RE14134 RE14157 RE15107 RE15378 RE15529 RE17347 RE17359 RE17418 RE17784 RE18344 RE21409 RE21417 RE21860 RE28756 RE29612 RE29858 RE32317 RE32724 RE34436 RE37161 RE38705 RE38878 RE39771 RE41261 RE42602 RE42706 RE43439 RE44593 RE46464 RE47526 RE51683 RE52372 RE53622 RE55082 RE58194 RE58473 RE60329 RE61225 RE61542 RE61818 RE64530 RE65065 RE65519 RE66139 RE66771 RE67008 RE68608 RE69280 RE71618 RE72391 RE74185 RE74886 RH37137 RH40176 RH47281 RH49390 RH65807 SD03595 SD05483 SD07981 SD08537 SD08821 SD10388 SD11117 SD11169 SD12167 SD14767 SD14847 SD16667 SD18068 SD20402 SD21433
Other clones	10064 13370 31705 33989 34084 34523 46619 46858 56247 57657 60459 60547 60793 65579 67195 80200 108850 117445 120331 153199 156029 162931 190419 191144 276832 281986 522668 542466 CK01.15C.D3 CK01.38A.A10 CK01.50A.G4 CK01.53C.G9 CK01.85C.C10 CK01.87C.H2 CK01.87C.H3 CK01.130C.F12 CK01833 Dmel_neb_009966_0636_2913 Dmel_neb_013866_0362_2043 Dmel_neb_014751_0175_0431 EC03301 EC03601 EC24136 EC39727 EC39818 EC40086 EC40527 EK015614 EK040202 EK043325 EK062944 EK065306 EK069130 EK069161 EK100242 EK114825 EK120002 EK127909 EK135703 EK141435 EK144837 EK148427 EK165440 EK167743 EK171122 EK171301 EK174904 EK185862 EK191116 EK192410 EK196142 EK235222 EK245217 EK251546 EK256311 EK258833 EK267058 EK292628 EK293628 EK303417 EN02531 EN02905 EN10654 FGC004D02 FGH103B01 RC0-DL0001-140700-120-D12 RC0-DP0001-140700-022-C08 RC0-DP0001-140700-022-H12 RC0-DP0002-140700-011-H11 RE71312 RP003006427 RP003024931 RP003043375 RP003047332 RP003055231 RP003065016 RP003068355 RP003072434 RP003073781 RP003082417 RP003086644 RP003092229 RP003093921 RP003094467 RP003094711 RP003095209 RP003100084 RP003100216 RP003100588 RP003102213 RP003114779 RP003126813 RP003132145 RP003132775 RP003133945 RP003146432 RP003148374 RP003148835 RP003168137 RP003168467 RP003176867 RP003184150 RP003189525 RP003190671 RP003210970 RP003213408 RP003219102 RP003225895 RP003226169 RP003226193 RP003228088 RP003228383 RP003252654 RP003254162 RP003256537 RP003258725 RP003265361 RP003265911 RP003278456 RP003278867 RP003280154 RP003281554 RP003281665 RP003282857 RP003301838 RP003310864 RP003313925 RP003321875 RP003327060 RP003329382 RP003337749 RP003337888 RP003342042 RP003344254 RP003347659 RP003380139 RP003423482 RP003437244 RP003442077 RP003447412 RP003460142 RP003470575 RP003475152 RP003481278 RP003482255 RP003483186 RP003497214 RP003504813 RP003510575 RP003512331 RP003522369 RP003522542 RP003527011 RP003534944 RP003541334 RP003543694 RP003558848 RP003568108 RP003594983 RP003603456 RP003624787 RP003632754 RP003633343 RP003637937 RP003651587 RP003655512 RP003657188 RP003661553 RP003670403 RP004009562 RP004088029 RP004099246 RP004110277 RP004120595 RP004145220 RP004213358 RP004219237 RP004221663 RP004221710 RP004235725 RP004240479 RP004253131 RP004262233 RP004281807 RP004313763 RP004325270 RP004348060 RP004402088 RP004442928 RP004511097 RP004512733 RP004519966 RP004529773 RP004546317 RP004555618 RP004557336
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0001218 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32133
Antibody Information
	polyclonal (Ryoo et al., 2007)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Hsc70-3 anon-WO0138581.7 (FlyBase, 1996-) Source for merge of: Hsc70-3 CG1579 (Whitfield, 2000.12.15) Source for merge of: Hsc70-3 l(1)G0102 l(1)G0341 l(1)G0111 l(1)G0292 l(1)G0407 (Peter et al., 2002)
Additional comments	Source for merge of Hsc70-3 anon-WO0138581.7 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	Hsc70-3 can substitute for its mammalian counterpart and chaperone the secretion of active IgGs (human monoclonal antibody heavy and light chains expressed in Schneider cells). Hsc70-3 can also uniquely chaperone heavy chain dimers indicating mechanistic differences that may relate to the evolved need for retaining immature IgGs in vertebrates. (Kirkpatrick et al., 1995) Members of the hsc70 gene family (heat shock cognate genes) that reside within the same intracellular compartment in different organisms share greater amino acid identity than hsc70 proteins from the same organism but different organelles. This pattern of conservation indicates specialisation of hsc70 function. (Rubin et al., 1993) Hsc70-3 genomic and cDNA clones have been analyzed and sequenced. (Rubin et al., 1990)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078577 NM_167306 NM_167307 NM_167308 RefSeq (Proteins) • NP_511132 NP_727563 NP_727564 NP_727565 DDBJ / EMBL / GenBank • AAA28626 AAF48095 AAN09299 AAN09300 AAN09301 AAO45194 AJ426871 AJ426872 AJ426873 AJ426875 AJ426876 AJ569733 AJ569734 AJ569735 AJ569736 AJ569737 AJ569738 AJ569739 AJ569740 AJ569741 AJ569742 AJ569744 AJ569745 AJ569746 AJ569747 AJ569748 AJ569749 AJ569750 AJ569751 AJ569752 AJ569753 AJ569755 AJ569756 AJ569757 AJ569758 AX153656 BI582973 BT004838 CL868484 CZ472321 CZ472365 CZ473280 CZ473281 CZ473771 CZ474241 L01498 L01499 Entrez Gene - A searchable database of RefSeq genes. • 32133 UniProtKB/Swiss-Prot • P29844
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Heat shock protein 70, conserved site (IPR018181) Heat shock protein 70 family (IPR013126) MitoDrome - A database of annotated Dmel nuclear genes encoding mitochondrial proteins • MTDROME04147 BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RH21402
Linkouts
	BioGRID - A database of protein and genetic interactions • 58539 DroID - A comprehensive database of gene and protein interactions. • FBgn0001218 DRSC - Results from RNAi screens. • FBgn0001218 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001218 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4147-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0001218 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32133 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32133 modMine - Data generated by the modENCODE project. • FBgn0001218
Synonyms & Secondary IDs ( 36 )
Reported As
Symbol Synonym	A4V4C4_DROME (Fukuyama et al., 2012) anon-WO0138581.7   BiP (Cirelli, 2003, Lecuit and Wieschaus, 2000, Shaw et al., 2000, Kirkpatrick et al., 1995, Naidoo et al., 2006, Hollien and Weissman, 2006, Gallet et al., 2006, Bayat et al., 2012, Tsruya et al., 2007, Plongthongkum et al., 2007, Challa et al., 2007, Yogev et al., 2008, Tsuda et al., 2008, Orso et al., 2009, Snee and Macdonald, 2009, Greene et al., 2005, Khanna et al., 2010, Maynard et al., 2010, Singh et al., 2010, Han et al., 2004, Neumüller et al., 2012) Bip (Kramer and Hawley, 2003, The et al., 1999, Liu et al., 2009) bip (Behr et al., 2007) CG1579 (Hughes et al., 2008) CG4147 (Jauch et al., 2002, Bourbon et al., 2002, Beller et al., 2006, Jauch et al., 2006, Cermelli et al., 2006, Kirienko and Fay, 2010, Worringer et al., 2009, Keleman et al., 2009.8.5, Hughes et al., 2008, Chen et al., 2008, Ni et al., 2010.12.1, Walker et al., 2006, Cammarato et al., 2011, Kanuka et al., 2005) dhsc70 (Rabindran et al., 1994) dmHsc72 (Ahmed and Duncan, 2004) Grp78 (Ahmed and Duncan, 2004, Muhlig-Versen et al., 2005) hsc3 (Farkas et al., 2007, Ryoo et al., 2006, Farkas et al., 2008, Ryoo et al., 2007) HSC3 (Saurin et al., 2001, Elefant and Palter, 1997, Elefant and Palter, 1995, Kirkpatrick et al., 1995, Rubin et al., 1993, Park et al., 2011) Hsc3 (Rubin, 1992, Rubin, 1992, Rubin et al., 1990, Wells and Palter, 1991, Rubin et al., 1990, Bayat et al., 2012, Tien et al., 2008, Tsuda et al., 2008, Mendes et al., 2009) Hsc-3 (Nikolaidis and Nei, 2004) Hsc3p (Elefant and Palter, 1999) Hsc70 (Wellington and Zinsmaier, 2005, Tatar et al., 2003, Yue et al., 1999, Wilke et al., 1994, Fredriksson et al., 2012) HSC-70 (Richmond and Broadie, 2002) HSC70 (Gunawardena et al., 2003, Singh and Lakhotia, 1995, Folk et al., 2006) hsc70 (Mehta et al., 1995, Ornelles and Penman, 1990) Hsc-70-3 (Conant and Wagner, 2003) hsc70-3 (Ringrose et al., 2003, Shaw and Franken, 2003, Norry et al., 2009, Kuronen et al., 2009, Behr et al., 2007, Zhang et al., 2010) Hsc70-3 (Beller et al., 2006, Beller et al., 2006, Cermelli et al., 2006, Dorner et al., 2006, Yamakawa et al., 2012, Katanayeva et al., 2010, Ravi Ram and Wolfner, 2007, Gruenewald et al., 2009, Buff et al., 2007, Walser et al., 2006, Gallach et al., 2010, Kallappagoudar et al., 2010, Brandt et al., 2008, Bettencourt et al., 2008, Worringer et al., 2009, Branco et al., 2008, Chen et al., 2008, Loewen and Feany, 2010, Wasbrough et al., 2010, Walker et al., 2006, Müller et al., 2010, Simonsen et al., 2008, Xun et al., 2008, Rehwinkel et al., 2004, Kanuka et al., 2005, Cook et al., 2010) HSC70-3 (Singh et al., 2010) hsc72 (Kirkpatrick et al., 1995, Mehta et al., 1991) HSP7C_DROME (Fukuyama et al., 2012) Hsp-c3 (Fisher et al., 2008) l(1)G0102 (Schaefer et al., 1999.8.12, Cook et al., 2010) l(1)G0111 (Schaefer et al., 1999.8.12, ) l(1)G0292 (Schaefer et al., 1999.8.12, ) l(1)G0341 (Schaefer et al., 1999.12.2, ) l(1)G0407 (Schaefer et al., 1999.8.12, ) l(1)G0466
Name Synonym	Heat shock 70-kDa protein cognate 3 (Pflieger et al., 2008, ) heat shock cognate protein 3 (Cermelli et al., 2006, Ryoo et al., 2007) Heat shock protein cognate 3 (Cammarato et al., 2011) immunoglobulin binding protein (Naidoo et al., 2006)
Secondary FlyBase IDs
	FBgn0027308 FBgn0027310 FBgn0028286 FBgn0028330 FBgn0029049 FBgn0030335 FBgn0040174 FBgn0044787
References ( 140 )
Generate a list of	All references relating to this gene ( 140 )
List References by type	Research paper  ( 84 ) Supplementary material  ( 12 ) Review  ( 6 ) Personal communication to FlyBase  ( 15 ) Abstract  ( 14 ) FlyBase analysis  ( 4 ) DNA/RNA sequence record  ( 2 ) Protein sequence record  ( 1 ) Curated genome annotation  ( 1 ) Patent  ( 1 )
Recent research papers ( 9 )
	Bayat et al., 2012, PLoS Biol. 10(3): e1001288 Mutations in the Mitochondrial Methionyl-tRNA Synthetase Cause a Neurodegenerative Phenotype in Flies and a Recessive Ataxia (ARSAL) in Humans. [FBrf0217845] Crawford et al., 2012, Cell Death Differ. 19(12): 2040--2048 Conservation of caspase substrates across metazoans suggests hierarchical importance of signaling pathways over specific targets and cleavage site motifs in apoptosis. [FBrf0220233] Defays and Bertoli, 2012, Alcohol 46(8): 737--745 Quantitative trait loci for response to ethanol in an intercontinental set of recombinant inbred lines of Drosophila melanogaster. [FBrf0220080] Fredriksson et al., 2012, Aging Cell 11(4): 634--643 Effects of aging and reproduction on protein quality control in soma and gametes of Drosophila melanogaster. [FBrf0218923] Fukuyama et al., 2012, J. Proteomics 75(15): 4610--4619 On-bead tryptic proteolysis: An attractive procedure for LC-MS/MS analysis of the Drosophila caspase 8 protein complex during immune response against bacteria. [FBrf0218992] Neumüller et al., 2012, Genetics 190(3): 931--940 Stringent analysis of gene function and protein-protein interactions using fluorescently tagged genes. [FBrf0217811] Yamakawa et al., 2012, Development 139(3): 558--567 Deficient Notch signaling associated with neurogenic pecanex is compensated for by the unfolded protein response in Drosophila. [FBrf0217160] Dottorini et al., 2011, BMC Bioinformatics 12: 34 Gepoclu: a software tool for identifying and analyzing gene positional clusters in large-scale gene expression analysis. [FBrf0214379] Park et al., 2011, Biochem. Biophys. Res. Commun. 404(2): 638--645 Normal prion protein in Drosophila enhances the toxicity of pathogenic polyglutamine proteins and alters susceptibility to oxidative and autophagy signaling modulators. [FBrf0212784] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...