FB2025_02 , released April 17, 2025
Gene: Dmel\Hsc70-5
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General Information
Symbol
Dmel\Hsc70-5
Species
D. melanogaster
Name
Heat shock protein 70 cognate 5
Annotation Symbol
CG8542
Feature Type
protein_coding_gene
FlyBase ID
FBgn0001220
Gene Model Status
Current
Stock Availability
12 publicly available
Enzyme Name (EC)
mitochondrial protein-transporting ATPase (7.4.2.3)
Gene Summary
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

Hsc70, Hsc5, Heat shock protein cognate 5, l(2)k04907, Heat shock 70 kDa protein cognate 5

Key Links
Genomic Location
Cytogenetic map
50E6-50E6
Sequence location
Recombination map
2-69
RefSeq locus
NT_033778 REGION:14252598..14256192
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (20 terms)
Molecular Function (8 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (8 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR012725, InterPro:IPR013126
(InterPro Project Members, 2004-)
enables ATP hydrolysis activity
inferred from biological aspect of ancestor with PANTHER:PTN000452648
(GO Reference Genome Project, 2011-)
enables ATP-dependent protein folding chaperone
inferred from electronic annotation with InterPro:IPR013126
(InterPro Project Members, 2004-)
enables enzyme regulator activity
inferred from sequence or structural similarity with UniProtKB:P0CS90
(Gene Ontology Curators, 2002-)
enables heat shock protein binding
inferred from biological aspect of ancestor with PANTHER:PTN000452648
(GO Reference Genome Project, 2011-)
enables mitochondrial protein-transporting ATPase activity
inferred from sequence or structural similarity with UniProtKB:P0CS90
(Gene Ontology Curators, 2002-)
enables protein folding chaperone
inferred from biological aspect of ancestor with PANTHER:PTN000452648
(GO Reference Genome Project, 2011-)
enables unfolded protein binding
inferred from electronic annotation with InterPro:IPR012725
(InterPro Project Members, 2004-)
Biological Process (8 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in mitochondrion organization
inferred from mutant phenotype
(Banerjee and Chinthapalli, 2014)
involved_in positive regulation of mitochondrial membrane potential
inferred from mutant phenotype
(Banerjee and Chinthapalli, 2014)
Terms Based on Predictions or Assertions (6 terms)
CV Term
Evidence
References
involved_in [2Fe-2S] cluster assembly
non-traceable author statement
(Marelja et al., 2018)
involved_in chaperone cofactor-dependent protein refolding
inferred from biological aspect of ancestor with PANTHER:PTN000452648
(GO Reference Genome Project, 2011-)
involved_in iron-sulfur cluster assembly
inferred from biological aspect of ancestor with PANTHER:PTN000452554
(GO Reference Genome Project, 2011-)
involved_in protein folding
inferred from electronic annotation with InterPro:IPR012725
(InterPro Project Members, 2004-)
involved_in protein import into mitochondrial matrix
inferred from sequence or structural similarity with UniProtKB:P0CS90
(Gene Ontology Curators, 2002-)
involved_in protein refolding
inferred from biological aspect of ancestor with PANTHER:PTN000452648
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity with UniProtKB:P0CS90
(Gene Ontology Curators, 2002-)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in mitochondrion
inferred from high throughput direct assay
(Tan et al., 2009)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
is_active_in cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN002321897
(GO Reference Genome Project, 2011-)
is_active_in mitochondrial matrix
non-traceable author statement
(Marelja et al., 2018)
inferred from sequence or structural similarity with UniProtKB:P38646
(Gene Ontology Curators, 2002-)
is_active_in mitochondrion
inferred from biological aspect of ancestor with PANTHER:PTN000452554
(GO Reference Genome Project, 2011-)
part_of PAM complex, Tim23 associated import motor
inferred from sequence or structural similarity with UniProtKB:P0CS90
(Gene Ontology Curators, 2002-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the heat shock protein 70 family. (P29845)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
mitochondrial protein-transporting ATPase activity
ATP + H2O + mitochondrial-proteinSide 1 = ADP + phosphate + mitochondrial-proteinSide 2 (7.4.2.3)
ATP hydrolysis activity
RHEA 13065:
Summaries
Gene Group (FlyBase)
HEAT SHOCK PROTEIN 70 CHAPERONES -
The Heat Shock Protein 70 (Hsp70) superfamily of chaperones assist in numerous folding processes and are upregulated by heat stress and toxic chemicals. Hsp70 chaperones share a highly conserved bipartite domain structure composed of an ATPase domain and a substrate-binding domain. (Adapted from PMID:17441502, FBrf0232269 and FBrf0174945).
PROTEIN-TRANSPORTING ATPASES -
Protein-transporting ATPases are the catalytic components of protein complexes that transport nuclear encoded (pre)proteins into organelles (eg. endoplasmic reticulum (ER), mitochondria) in an ATP-dependent manner. They include: the mitochondrial Hsp70 protein within the TIM complex that transports proteins across the inner mitochondrial membrane into the matrix; the Bsc1 protein that forms a homo-heptameric channel to translocate the iron-sulfur protein, a subunit of respiratory Complex III, across the inner mitochondrial membrane; and the BiP chaperone that provides the driving force for posttranslational translocation of proteins across the ER membrane via the Sec61 channel. (Adapted from PMID:12154367, PMID:32042153 and PMID:28564553.)
PRESEQUENCE TRANSLOCASE-ASSOCIATED MOTOR SUBUNITS -
The presequence translocase-associated motor (PAM) is a subcomplex of the TIM23 complex. It is required to drive the ATP hydrolysis-dependent translocation of proteins across the inner mitochondrial membrane for import into the matrix. (Adapted from PMID:32074073.)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Hsc70-5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry P29845)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.50

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Hsc70-5-RA
FBtr0087568
NM_079017
2922
686
Hsc70-5-RB
FBtr0339969
NM_001299485
2791
686
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Hsc70-5-PA
FBpp0086694
74.1
686
6.25
P29845
NP_523741
AAF58270
Hsc70-5-PB
FBpp0308990
74.1
686
6.25
P29845
NP_001286414
AHN56212
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

686 aa isoforms: Hsc70-5-PA, Hsc70-5-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Hsc70-5 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

3.46

Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in mitochondrion
inferred from high throughput direct assay
(Tan et al., 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Hsc70-5 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 7-8
  • Stages(s) 11-12
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 7 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Hsc70-5
Transgenic constructs containing regulatory region of Hsc70-5
Aberrations (Deficiencies and Duplications) ( 3 )
Inferred from experimentation ( 3 )
Gene disrupted in
Df(2R)BSC134
(Christensen and Cook, 2006.3.8)
Df(2R)CX1
(BDGP Project Members, 1994-1999)
Df(2R)Exel7131
(Ryder, 2004.4.26)
Inferred from location ( 4 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4Act5C.PI
lethal, with Scer\GAL4c564
lethal, with Scer\GAL4tin.CΔ4
lethal | paternal effect, with Scer\GAL4VP16.bam
lethal | recessive
lethal - all die before end of P-stage, with Scer\GAL4ey.PH
lethal - all die before end of pupal stage, with Scer\GAL4elav.PU
lethal - all die before end of pupal stage, with Scer\GAL4pnr-MD237
lethal - all die during larval stage, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4ey.PU
viable, with Scer\GAL4GMR.PF
viable, with Scer\GAL4Mhc.PU
Sterility
Allele
female sterile, with Scer\GAL4nanos.PG
Other Phenotypes
Allele
abnormal heat stress response | adult stage, with Scer\GAL4elav.PU
abnormal locomotor behavior | adult stage, with Scer\GAL4elav.PU
abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4elav-C155
abnormal locomotor behavior | third instar larval stage, with Scer\GAL4elav.PU
abnormal neurophysiology | third instar larval stage, with Scer\GAL4elav.PU
decreased rate of movement | third instar larval stage, with Scer\GAL4elav.PU
decreased size | spermatogenesis, with Scer\GAL4VP16.bam
increased cell death, with Scer\GAL4NP5130
majority die during pupal stage, with Scer\GAL4Ddc.PL
majority die during pupal stage, with Scer\GAL4elav-C155
majority die during pupal stage, with Scer\GAL4ple.PF
majority die during pupal stage, with Scer\GAL4Toll-6-D42
majority die during pupal stage, with Scer\GAL4VGlut1-OK371
majority die during second instar larval stage, with Scer\GAL4Act5C.PI
majority die during second instar larval stage, with Scer\GAL4RapGAP1-OK6
majority die during second instar larval stage, with Scer\GAL4Toll-6-D42
majority die during second instar larval stage, with Scer\GAL4VGlut1-OK371
majority die during third instar larval stage, with Scer\GAL4RapGAP1-OK6
paralytic | adult stage | heat sensitive, with Scer\GAL4elav.PU
short lived, with Scer\GAL4elav.PU
short lived | temperature conditional, with Scer\GAL4elav-C155
some die during pupal stage, with Scer\GAL4pnr-MD237
visible | adult stage, with Scer\GAL4elav.PU
visible | adult stage, with Scer\GAL4elav-C155
Phenotype manifest in
Allele
adult midgut progenitor cell, with Scer\GAL4NP5130
embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4elav.PU
microtubule | third instar larval stage, with Scer\GAL4elav.PU
microtubule | third instar larval stage, with Scer\GAL4elav-C155
mitochondrion, with Scer\GAL4elav-C155
mitochondrion, with Scer\GAL4Tub.PU
mitochondrion | decreased number | third instar larval stage, with Scer\GAL4elav.PU
mitochondrion | third instar larval stage, with Scer\GAL4elav.PU
neuromuscular junction | third instar larval stage, with Scer\GAL4elav-C155
NMJ bouton | third instar larval stage, with Scer\GAL4elav.PU
spermatocyte cyst, with Scer\GAL4VP16.bam
spermatozoon | absent, with Scer\GAL4VP16.bam
terminal bouton | third instar larval stage, with Scer\GAL4elav-C155
testis, with Scer\GAL4VP16.bam
wing, with Scer\GAL4elav.PU
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (21)
Hsap\HSPA9
14 of 14
Yes
Yes
 
8  
Hsap\HSPA1A
4 of 14
No
No
4  
Hsap\HSPA1B
4 of 14
No
No
Hsap\HSPA1L
4 of 14
No
No
11  
Hsap\HSPA2
4 of 14
No
No
1  
Hsap\HSPA5
4 of 14
No
No
1  
Hsap\HSPA6
4 of 14
No
No
Hsap\HSPA8
4 of 14
No
No
1  
Hsap\HSPA13
2 of 14
No
No
Hsap\ANKRD45
1 of 14
No
Yes
Hsap\DENND6A
1 of 14
No
No
1  
Hsap\DENND6B
1 of 14
No
No
Hsap\HSPA4
1 of 14
No
No
1  
Hsap\HSPA4L
1 of 14
No
No
1  
Hsap\HSPA7
1 of 14
No
Yes
Hsap\HSPA12A
1 of 14
No
No
Hsap\HSPA12B
1 of 14
No
No
Hsap\HSPA14
1 of 14
No
No
Hsap\HSPH1
1 of 14
No
No
1  
Hsap\HYOU1
1 of 14
No
No
1  
Hsap\SLC9C2
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (17)
Rnor\Hspa9
14 of 14
Yes
Yes
Rnor\Hspa1l
4 of 14
No
No
Rnor\Hspa2
4 of 14
No
No
Rnor\Hspa5
4 of 14
No
No
Rnor\Hspa8
4 of 14
No
No
Rnor\Hspa1a
3 of 14
No
No
Rnor\Hspa1b
3 of 14
No
No
Rnor\LOC680121
2 of 14
No
No
Rnor\Hsc70-ps1
1 of 14
No
No
Rnor\Hspa4
1 of 14
No
No
Rnor\Hspa12a
1 of 14
No
No
Rnor\Hspa12b
1 of 14
No
No
Rnor\Hspa13
1 of 14
No
No
Rnor\Hspa14
1 of 14
No
No
Rnor\Hsph1
1 of 14
No
No
Rnor\Hyou1
1 of 14
No
No
Rnor\LOC689955
1 of 14
No
Yes
Mus musculus (laboratory mouse) (15)
Mmus\Hspa9
14 of 14
Yes
Yes
Mmus\Hspa1a
4 of 14
No
No
Mmus\Hspa1b
4 of 14
No
No
Mmus\Hspa1l
4 of 14
No
No
Mmus\Hspa2
4 of 14
No
No
Mmus\Hspa8
4 of 14
No
No
Mmus\Hspa5
3 of 14
No
No
Mmus\Hspa13
2 of 14
No
No
Mmus\Ankrd45
1 of 14
No
Yes
Mmus\Hspa4
1 of 14
No
No
Mmus\Hspa12a
1 of 14
No
No
Mmus\Hspa12b
1 of 14
No
No
Mmus\Hspa14
1 of 14
No
No
Mmus\Hsph1
1 of 14
No
No
Mmus\Hyou1
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (15)
Xtro\hspa9
11 of 13
Yes
Yes
Xtro\hspa1a
3 of 13
No
No
Xtro\hspa1l
3 of 13
No
No
Xtro\hspa8
3 of 13
No
No
Xtro\hsp70
2 of 13
No
No
Xtro\hspa1b
2 of 13
No
No
Xtro\hspa2
2 of 13
No
No
Xtro\hspa5
2 of 13
No
No
Xtro\tut7
2 of 13
No
No
Xtro\dennd6b
1 of 13
No
No
Xtro\elp2
1 of 13
No
No
Xtro\hspa13
1 of 13
No
Yes
Xtro\prelid2
1 of 13
No
Yes
Xtro\rlf
1 of 13
No
Yes
Xtro\XB5934250
1 of 13
No
No
Danio rerio (Zebrafish) (20)
Drer\hspa9
14 of 14
Yes
Yes
Drer\hsc70
4 of 14
No
No
Drer\hsp70.1
4 of 14
No
No
Drer\hsp70.3
4 of 14
No
No
Drer\hsp70l
4 of 14
No
No
0  
Drer\hspa8b
4 of 14
No
No
Drer\hspa1b
3 of 14
No
No
Drer\hspa5
3 of 14
No
No
Drer\hspa8
3 of 14
No
No
Drer\hsp70.2
2 of 14
No
No
Drer\hspa13
2 of 14
No
No
Drer\ankrd45
1 of 14
No
Yes
Drer\hspa4a
1 of 14
No
No
Drer\hspa4b
1 of 14
No
No
Drer\hspa12a.2
1 of 14
No
Yes
Drer\hspa12a.3
1 of 14
No
No
Drer\hspa12a
1 of 14
No
No
Drer\hspa14
1 of 14
No
No
Drer\hsph1
1 of 14
No
No
Drer\hyou1
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (12)
Cele\hsp-6
14 of 14
Yes
Yes
Cele\F44E5.4
4 of 14
No
No
Cele\F44E5.5
4 of 14
No
No
Cele\hsp-1
4 of 14
No
No
Cele\hsp-3
4 of 14
No
No
Cele\hsp-4
4 of 14
No
No
Cele\hsp-70
4 of 14
No
No
Cele\stc-1
2 of 14
No
No
Cele\F11F1.1
1 of 14
No
No
Cele\F53H1.3
1 of 14
No
No
Cele\hsp-110
1 of 14
No
No
Cele\T24H7.2
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (12)
Agam\AgaP_AGAP010876
12 of 12
Yes
Yes
Agam\AgaP_AGAP002076
4 of 12
No
No
Agam\AgaP_AGAP004192
4 of 12
No
No
Agam\AgaP_AGAP004583
4 of 12
No
No
Agam\AgaP_AGAP004944
4 of 12
No
No
Agam\AgaP_AGAP004581
3 of 12
No
No
Agam\AgaP_AGAP007682
3 of 12
No
No
Agam\AgaP_AGAP001827
1 of 12
No
No
Agam\AgaP_AGAP004582
1 of 12
No
No
Agam\AgaP_AGAP004946
1 of 12
No
No
Agam\AgaP_AGAP010331
1 of 12
No
No
Agam\AgaP_AGAP012891
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (21)
Atha\mtHsc70-1
13 of 13
Yes
Yes
Atha\MTHSC70-2
12 of 13
No
Yes
Atha\cpHsc70-1
6 of 13
No
Yes
Atha\cpHsc70-2
6 of 13
No
Yes
Atha\AT3G09440
4 of 13
No
No
Atha\BIP1
4 of 13
No
No
Atha\BIP2
4 of 13
No
No
Atha\BIP3
4 of 13
No
No
Atha\ERD2
4 of 13
No
No
Atha\HSC70-1
4 of 13
No
No
Atha\Hsp70-2
4 of 13
No
No
Atha\HSP70
4 of 13
No
No
Atha\Hsp70b
4 of 13
No
No
Atha\AT4G32208
3 of 13
No
Yes
Atha\HSP70T-2
3 of 13
No
Yes
Atha\AT1G11660
1 of 13
No
No
Atha\AT4G16660
1 of 13
No
No
Atha\Hsp70-15
1 of 13
No
No
Atha\HSP91
1 of 13
No
No
Atha\SDH3-1
1 of 13
No
No
Atha\SDH3-2
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (14)
Scer\SSC1
13 of 13
Yes
Yes
Scer\ECM10
12 of 13
No
Yes
Scer\SSQ1
6 of 13
No
Yes
Scer\KAR2
3 of 13
No
No
Scer\SSA1
3 of 13
No
No
Scer\SSA2
3 of 13
No
No
Scer\SSA3
3 of 13
No
No
Scer\SSA4
3 of 13
No
No
Scer\SSB1
3 of 13
No
No
Scer\SSB2
3 of 13
No
No
Scer\LHS1
1 of 13
No
No
Scer\SSE1
1 of 13
No
No
Scer\SSE2
1 of 13
No
No
Scer\SSZ1
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (8)
Spom\ssc1
12 of 12
Yes
Yes
Spom\bip1
3 of 12
No
No
Spom\ssa1
3 of 12
No
No
Spom\ssa2
3 of 12
No
No
Spom\sks2
2 of 12
No
No
Spom\lsh1
1 of 12
No
No
Spom\pss1
1 of 12
No
No
Spom\SPAC57A7.12
1 of 12
No
No
Escherichia coli (enterobacterium) (4)
Ecol\dnaK
10 of 11
Yes
Yes
Ecol\hscA
3 of 11
No
Yes
Ecol\hscC
3 of 11
No
Yes
Ecol\yegD
2 of 11
No
No
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Hsc70-5. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (15)
Hsc70-1
6 of 13
Hsc70-2
6 of 13
Hsc70-3
6 of 13
Hsc70-4
6 of 13
Hsp68
6 of 13
Hsp70Ab
6 of 13
Hsp70Bbb
6 of 13
Hsp70Aa
5 of 13
Hsp70Ba
5 of 13
Hsp70Bb
5 of 13
Hsp70Bc
5 of 13
CG7182
3 of 13
Grp170
2 of 13
Hsp110
2 of 13
CG3309
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 2 )
Allele
Disease
Evidence
References
Hsc70-5GD13957
model of  Parkinson's disease
CEA
(Zhu et al., 2021)
Hsc70-5KK100233
model of  Parkinson's disease
CEA
(Zhu et al., 2021)
Potential Models Based on Orthology ( 1 )
Modifiers Based on Experimental Evidence ( 2 )
Allele
Disease
Interaction
References
Hsc70-5KK100233
ameliorates  cancer
modeled by DeltaUAS.cDa, lolaGS88A8, psqGS88A8
(Even et al., 2019)
ameliorates  amyotrophic lateral sclerosis type 6
modeled by Hsap\FUSWT.UAS.RFP(Unk),Tag:HA
(Deng et al., 2018)
exacerbates  amyotrophic lateral sclerosis type 6
modeled by Hsap\FUSWT.UAS.RFP(Unk),Tag:HA
(Deng et al., 2018)
model of  Parkinson's disease
is ameliorated by LrrkHMS00456
(Zhu et al., 2021)
is exacerbated by RopHM05140
(Zhu et al., 2021)
is exacerbated by RphJF01970
(Zhu et al., 2021)
is exacerbated by Sec15JF02649
(Zhu et al., 2021)
is exacerbated by Snap25JF02615
(Zhu et al., 2021)
is exacerbated by SytβJF02593
(Zhu et al., 2021)
is ameliorated by Atg1JF02273
(Zhu et al., 2021)
is exacerbated by Atg1UAS.cSa
(Zhu et al., 2021)
is ameliorated by Atg101HMS01349
(Zhu et al., 2021)
is ameliorated by Atg12JF02704
(Zhu et al., 2021)
is exacerbated by Syx7JF02436
(Zhu et al., 2021)
is exacerbated by VGlut1JF02689
(Zhu et al., 2021)
is ameliorated by Vps15GL00085
(Zhu et al., 2021)
is ameliorated by Atg5JF02703
(Zhu et al., 2021)
is exacerbated by brpRNAi.UAS.cUa
(Zhu et al., 2021)
is ameliorated by dj-1βJF01202
(Zhu et al., 2021)
is exacerbated by tkoHMS01695
(Zhu et al., 2021)
is ameliorated by Atg7JF02787
(Zhu et al., 2021)
is ameliorated by Atg8aHMS01328
(Zhu et al., 2021)
is ameliorated by BuffyRNAi.UAS
(Zhu et al., 2021)
is ameliorated by DCTN1-p150JF02803
(Zhu et al., 2021)
is ameliorated by DCTN2-p50HM05084
(Zhu et al., 2021)
is exacerbated by EndoAJF02758
(Zhu et al., 2021)
is exacerbated by Eps-15JF03257
(Zhu et al., 2021)
is ameliorated by Hsap\HSPA9UAS.cZa
(Zhu et al., 2021)
Hsc70-5UAS.cPa
ameliorates  neurodegenerative disease
modeled by Afg3l2GD3606
(Pareek and Pallanck, 2020)
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
HSPA9; heat shock protein family A (Hsp70) member 9
14 of 14
600548
 
HSPA1A; heat shock protein family A (Hsp70) member 1A
4 of 14
140550
       
      HSPA1B; heat shock protein family A (Hsp70) member 1B
      4 of 14
      603012
          HSPA1L; heat shock protein family A (Hsp70) member 1 like
          4 of 14
          140559
               
              HSPA2; heat shock protein family A (Hsp70) member 2
              4 of 14
              140560
                   
                  HSPA5; heat shock protein family A (Hsp70) member 5
                  4 of 14
                  138120
                       
                      HSPA6; heat shock protein family A (Hsp70) member 6
                      4 of 14
                      140555
                          HSPA8; heat shock protein family A (Hsp70) member 8
                          4 of 14
                          600816
                               
                              Functional Complementation Data
                              Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                              Dmel gene
                              Ortholog showing functional complementation
                              Supporting References
                              Hsc70-5
                              Hsap\HSPA9
                              (Zhu et al., 2021)
                              Interactions
                              Summary of Physical Interactions
                              Summary of Genetic Interactions
                              Interaction Browsers
                              View in FlyBase Interactions Browser   View in MIST tool (external link)
                              Please look at the allele data for full details of the genetic interactions
                              Starting gene(s)
                              Interaction type
                              Interacting gene(s)
                              Reference
                              Hsc70-5
                              enhanceable
                              Atg1
                              (Zhu et al., 2021)
                              Hsc70-5
                              suppressible
                              Atg1
                              (Zhu et al., 2021)
                              Hsc70-5
                              suppressible
                              Atg5
                              (Zhu et al., 2021)
                              Hsc70-5
                              suppressible
                              Atg7
                              (Zhu et al., 2021)
                              Hsc70-5
                              suppressible
                              Atg8a
                              (Zhu et al., 2021)
                              Hsc70-5
                              suppressible
                              Atg12
                              (Zhu et al., 2021)
                              Hsc70-5
                              suppressible
                              Atg101
                              (Zhu et al., 2021)
                              Hsc70-5
                              enhanceable
                              brp
                              (Zhu et al., 2021)
                              Hsc70-5
                              suppressible
                              Buffy
                              (Zhu et al., 2021)
                              Hsc70-5
                              suppressible
                              DCTN1-p150
                              (Zhu et al., 2021)
                              Hsc70-5
                              suppressible
                              DCTN2-p50
                              (Zhu et al., 2021)
                              Hsc70-5
                              suppressible
                              dj-1β
                              (Zhu et al., 2021)
                              Hsc70-5
                              suppressible
                              dor
                              (Banerjee and Chinthapalli, 2014)
                              Hsc70-5
                              suppressible
                              Drp1
                              (Banerjee and Chinthapalli, 2014)
                              Hsc70-5
                              enhanceable
                              EndoA
                              (Zhu et al., 2021)
                              Hsc70-5
                              enhanceable
                              Eps-15
                              (Zhu et al., 2021)
                              Hsc70-5
                              suppressible
                              Lrrk
                              (Zhu et al., 2021)
                              Hsc70-5
                              suppressible
                              Opa1
                              (Banerjee and Chinthapalli, 2014)
                              Hsc70-5
                              suppressible
                              Pink1
                              (Zhu et al., 2021)
                              Hsc70-5
                              enhanceable
                              Pink1
                              (Zhu et al., 2021)
                              Hsc70-5
                              enhanceable
                              Pka-C1
                              (Banerjee and Chinthapalli, 2014)
                              Hsc70-5
                              enhanceable
                              Rop
                              (Zhu et al., 2021)
                              Hsc70-5
                              enhanceable
                              Rph
                              (Zhu et al., 2021)
                              Hsc70-5
                              enhanceable
                              Sec15
                              (Zhu et al., 2021)
                              Hsc70-5
                              enhanceable
                              Snap25
                              (Zhu et al., 2021)
                              Hsc70-5
                              enhanceable
                              Sytβ
                              (Zhu et al., 2021)
                              Hsc70-5
                              enhanceable
                              Syx7
                              (Zhu et al., 2021)
                              Hsc70-5
                              enhanceable
                              tko
                              (Zhu et al., 2021)
                              Hsc70-5
                              enhanceable
                              VGlut1
                              (Zhu et al., 2021)
                              Hsc70-5
                              suppressible
                              Vps15
                              (Zhu et al., 2021)
                              Starting gene(s)
                              Interaction type
                              Interacting gene(s)
                              Reference
                              Afg3l2
                              suppressible
                              Hsc70-5
                              (Pareek and Pallanck, 2020)
                              Delta
                              suppressible
                              Hsc70-5
                              (Even et al., 2019)
                              Delta|lola|psq
                              suppressible
                              Hsc70-5
                              (Even et al., 2019)
                              External Data
                              Linkouts
                              BioGRID - A database of protein and genetic interactions.
                              DroID - A comprehensive database of gene and protein interactions.
                              MIST (protein-protein) - An integrated Molecular Interaction Database
                              Pathways
                              Signaling Pathways (FlyBase)
                              Metabolic Pathways
                              FlyBase
                              External Links
                              Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
                              External Data
                              Linkouts
                              KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                              Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                              Class of Gene
                              Genomic Location and Detailed Mapping Data
                              Chromosome (arm)
                              2R
                              Recombination map
                              2-69
                              Cytogenetic map
                              50E6-50E6
                              Sequence location
                              FlyBase Computed Cytological Location
                              Cytogenetic map
                              Evidence for location
                              50E6-50E6
                              Limits computationally determined from genome sequence between P{lacW}l(2)s3475s3475&P{lacW}Hsc70-5k04907 and P{PZ}Tfb106949&P{PZ}l(2)0356303563
                              Experimentally Determined Cytological Location
                              Cytogenetic map
                              Notes
                              References
                              50E4-50E7
                              (determined by in situ hybridisation)
                              (Spradling et al., 1999)
                              50E4-50E7
                              50E6--7
                              (BDGP Project Members, 1994-1999)
                              Experimentally Determined Recombination Data
                              Location

                              2-69

                              (FlyBase, 2016)

                              2-74.9

                              (Comeron et al., 2012)
                              Left of (cM)
                              Right of (cM)
                              Notes
                              Stocks and Reagents
                              Stocks (12)
                              Genomic Clones (18)
                               

                              Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

                              cDNA Clones (224)
                               

                              Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

                              cDNA clones, fully sequenced
                              BDGP DGC clones
                              Other clones
                                Drosophila Genomics Resource Center cDNA clones

                                For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                                cDNA Clones, End Sequenced (ESTs)
                                BDGP DGC clones
                                Other clones
                                RNAi and Array Information
                                Linkouts
                                DRSC - Results frm RNAi screens
                                Antibody Information
                                Laboratory Generated Antibodies
                                 
                                Commercially Available Antibodies
                                 
                                Cell Line Information
                                Publicly Available Cell Lines
                                 
                                  Other Stable Cell Lines
                                   
                                    Other Comments

                                    Identification: One of a collection of genes identified with defective larval growth that extend larval life. Not studied further due to problems with background/multiple lesion effects.

                                    (Galloni and Edgar, 1999)

                                    Members of the hsc70 gene family (heat shock cognate genes) that reside within the same intracellular compartment in different organisms share greater amino acid identity than hsc70 proteins from the same organism but different organelles. This pattern of conservation indicates specialisation of hsc70 function.

                                    (Rubin et al., 1993)

                                    Hsc70-5 is believed to encode a mitochondrial heat shock protein.

                                    (Rubin et al., 1990)
                                    Relationship to Other Genes
                                    Source for database merge of

                                    Source for merge of: Hsc70-5 l(2)k04907

                                    (Spradling et al., 1999)
                                    Additional comments
                                    Nomenclature History
                                    Source for database identify of
                                    Nomenclature comments
                                    Etymology
                                    Synonyms and Secondary IDs (31)
                                    Reported As
                                    Symbol Synonym
                                    CG8542
                                    (National Institute of Genetics Fly Stocks, 2022-, Kolkhof et al., 2017, Raut et al., 2017, Çiçek et al., 2016, Kumar et al., 2016, Hosono et al., 2015, Rosa-Ferreira et al., 2015, Banerjee and Chinthapalli, 2014, Benbahouche et al., 2014, Costa et al., 2013, Zhu et al., 2013, Yano et al., 2012, Cammarato et al., 2011, Keleman et al., 2009.8.5, Hughes et al., 2008, Derré et al., 2007, Quinones-Coello, 2007, Molnar et al., 2006)
                                    HSC-70
                                    (Richmond and Broadie, 2002)
                                    HSC5
                                    (Rubin et al., 1993)
                                    HSC70
                                    (Folk et al., 2006, Gunawardena et al., 2003, Singh and Lakhotia, 1995)
                                    HSC70-5
                                    (Costa et al., 2013, Kuo et al., 2013)
                                    HSP70m1
                                    (Kaneko, 2022)
                                    Hsc-5
                                    (Nikolaidis and Nei, 2004)
                                    Hsc-70-5
                                    (Wang et al., 2019)
                                    Hsc5
                                    (Gerrard et al., 1993, Rubin, 1992, Rubin, 1992, Rubin et al., 1990, Rubin et al., 1990)
                                    Hsc70
                                    (Himalian et al., 2022, Fredriksson et al., 2012, Wellington and Zinsmaier, 2005, Tatar et al., 2003, Yue et al., 1999, Wilke et al., 1994)
                                    Hsc70-5
                                    (Barone et al., 2025, Vesala et al., 2024, Everman et al., 2023, Xu et al., 2023, Yamada et al., 2023, Huang et al., 2022, Kaneko, 2022, Perlegos et al., 2022, Rai and Tapadia, 2022, Ribot et al., 2022, Azuma et al., 2021, Naz and Siddique, 2021, Sureka and Mishra, 2021, Zhu et al., 2021, Jo et al., 2020, Pareek and Pallanck, 2020, Wang et al., 2020, Even et al., 2019, Liaw and Chiang, 2019, Deng et al., 2018, Gene Disruption Project members, 2018-, Hall et al., 2018, Kucherenko and Shcherbata, 2018, Levis, 2018.8.30, Pareek et al., 2018, Galluzzi et al., 2017, Marcogliese et al., 2017, Yoon et al., 2017, Clandinin and Owens, 2016-, Dent et al., 2015, Štětina et al., 2015, Benbahouche et al., 2014, Luck et al., 2014, Mukherjee et al., 2014, Radermacher et al., 2014, Colinet et al., 2013, Czech et al., 2013, Kwon et al., 2013, Zhu et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Friedman et al., 2011, Fernández-Ayala et al., 2010, Zhang et al., 2010, Carbone et al., 2009, Takemori and Yamamoto, 2009, Tan et al., 2009, Bettencourt et al., 2008, Juhász et al., 2007, Quinones-Coello, 2007, Beller et al., 2006, Molnar et al., 2006, Neal et al., 2006, Walser et al., 2006, Birch-Machin et al., 2005, Rehwinkel et al., 2004)
                                    Hsp-c5
                                    (Fisher et al., 2008)
                                    dHSC70-5
                                    (Murari et al., 2022)
                                    dHsc70-5
                                    (Murari et al., 2023)
                                    dhsc70
                                    (Rabindran et al., 1994)
                                    hsc-70-5
                                    (Pimenta de Castro et al., 2012)
                                    hsc5
                                    (Kim et al., 2015)
                                    hsc70
                                    (Mehta et al., 1995, Mehta et al., 1991, Ornelles and Penman, 1990)
                                    hsc70-5
                                    (Banerjee and Chinthapalli, 2014, Owusu-Ansah et al., 2013, Zhang et al., 2010)
                                    l(2)k04907
                                    (Galloni and Edgar, 1999, BDGP Project Members, 1994-1999)
                                    mtHsc70
                                    (Liu et al., 2020)
                                    Name Synonyms
                                    70 kDa heat shock protein, mitochondrial 1
                                    (Kaneko, 2022)
                                    Heat Shock 70 kDa Protein Cognate 5
                                    (Costa et al., 2013)
                                    Heat shock 70 kDA protein Cognate 5
                                    (Urban et al., 2017)
                                    Heat shock 70 kDa protein cognate 5
                                    (Banerjee and Chinthapalli, 2014, Costa et al., 2013)
                                    Heat shock protein 70 cognate 5
                                    Heat shock protein cognate 5
                                    (Yano et al., 2012, Cammarato et al., 2011)
                                    Hsp cognate 5
                                    (Takemori and Yamamoto, 2009)
                                    Mortalin
                                    (Banerjee and Chinthapalli, 2014)
                                    heat shock protein cognate 71
                                    (Breloy et al., 2016)
                                    mortalin
                                    (Zhu et al., 2013)
                                    Secondary FlyBase IDs
                                    • FBgn0022196
                                    Datasets (0)
                                    Study focus (0)
                                    Experimental Role
                                    Project
                                    Project Type
                                    Title
                                    Study result (0)
                                    Result
                                    Result Type
                                    Title
                                    External Crossreferences and Linkouts ( 53 )
                                    Sequence Crossreferences
                                    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                                    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                                    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                                    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                                    UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                                    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                                    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                                    Other crossreferences
                                    AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                                    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                                    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                                    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                                    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                                    FlyMine - An integrated database for Drosophila genomics
                                    InterPro - A database of protein families, domains and functional sites
                                    KEGG Genes - Molecular building blocks of life in the genomic space.
                                    MARRVEL_MODEL - MARRVEL (model organism gene)
                                    Linkouts
                                    BioGRID - A database of protein and genetic interactions.
                                    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                                    DroID - A comprehensive database of gene and protein interactions.
                                    DRSC - Results frm RNAi screens
                                    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                                    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                                    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                                    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                                    Flygut - An atlas of the Drosophila adult midgut
                                    FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                                    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                                    KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                                    MIST (protein-protein) - An integrated Molecular Interaction Database
                                    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                                    Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
                                    References (160)