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Gene Dmel\Hsp22

General Information
Symbol	Dmel\Hsp22	Species	D. melanogaster
Name	Heat shock protein 22	Annotation symbol	CG4460
Feature type	protein_coding_gene	FlyBase ID	FBgn0001223
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	CG32041, DmHsp22
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	67B2-67B2	Sequence location	3L:9,366,031..9,368,064 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Heat shock protein 22 is referred to in FlyBase by the symbol Dmel\Hsp22 (CG4460, FBgn0001223). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: determination of adult lifespan; response to oxidative stress; response to heat. 12 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Alpha crystallin/Heat shock protein; HSP20-like chaperone; Heat shock protein Hsp20. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed during late pupal stages. This gene is annotated by FlyBase as a dicistronic gene, meaning that some or all of its transcripts encode two or more polypeptide-coding open reading frames (ORFs) , with each ORF assigned to a different gene. The distribution of RNA-Seq coverage data amongst the different encoded genes cannot be determined. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: larval/adult Malpighian tubules, larval salivary gland, adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, adult crop, larval hindgut, adult heart, larval fat body, adult ovary, adult carcass. Gene sequence location is 3L:9366031..9368064.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Hsp22 - Hsp-G3 There are seven closely related heat-shock genes at 67B (Ayme and Tissieres, 1985; Pauli, Arrigo, Vasquez, Tonka, and Tissieres, 1989, Genome 31: 671-76). In addition to the four small heat-shock genes previously identified (Hsp22, Hsp23, Hsp26, and Hsp27), three more genes (Hsp-G1, Hsp-G2, and Hsp-G3, formerly called Gene1, Gene2, and Gene3) have been found clustered within 15 kb of DNA at the same 67B cytological location. All seven genes are heat-shock inducible in almost all cells at the stages tested (Ayme and Tissieres, 1985). The genes are also transcribed during certain developmental stages in the absence of heat shock (Sirotkin and Davidson, 1982, Dev. Biol. 89: 196-210). Pauli et al (1989) report that the maximum accumulation of developmental rRNA in a majority of these small heat-shock genes occurs in the white pupae stage; in Hsp-G2, however, a small transcipt is found in embryos, first and second instar larvae, and young pupae; and a larger transcript in the pupal and adult stages of males (Pauli and Tonka, 1987, J. Mol. Biol. 198: 235-40; Pauli, Tonka, and Ayme-Southgate, 1988, J. Mol. Biol. 200: 47-53). In absence of stress, the expression of Hsp26 has been observed in spermatocytes, nurse cells, epithelium, imaginal discs, proventriculus, and neurocytes (Glaser, Wolfner, and Lis, 1986, EMBO 5: 747-54). Transcripts of Hsp26 and Hsp27 accumulate in adult ovaries, apparently originating in nurse cells (Zimmerman, Petri, and Meselson, 1983, Cell 32: 1161-70).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Transgenic Constructs P{GD8704}
FB2012_01	References Groebe et al., 2010, Exp. Gerontol. 45(7-8): 632--637 Kim et al., 2010, Exp. Gerontol. 45(12): 984--987
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 67B2-67B2   Limits computationally determined from genome sequence between P{PZ}l(3)0162901629&P{PZ}mRpL1210534 and P{EP}Hsp26EP3336&P{EP}Hsp26EP3315
Experimentally Determined Cytological Location
Cytogenetic map Notes References 67B-67B   (determined by in situ hybridisation)   (Corces et al., 1980)   A probe containing both Hsp22 and Hsp26 hybridises to cytological locations 67B1--67B2 and 67B4--67B5.   (Cuenca et al., 1998)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)	h (Peterson et al., 1979)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Hsp22 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	gene_with_dicistronic_mRNA ; SO:0000722
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Hsp22-RA FBtr0100558  NM_001031944   1900   174 Hsp22-RB FBtr0100559  NM_001031943   947   174
Additional Transcript Data & Comments
Reported size (kB)	1.03 (northern blot) (Corces et al., 1980)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Hsp22-PA   FBpp0100010   19.8   174   4.95     NP_001027115   AAF50290 Hsp22-PB   FBpp0100011   19.8   174   4.95     NP_001027114   AAN11962
Additional Polypeptide Data & Comments
Reported size (kDa)	174 (aa); 19.705 (kD predicted) (Southgate et al., 1983) 22 (kD observed) (Craig and McCarthy, 1980) 22.666 (kD predicted) (Ingolia and Craig, 1982)
Comments	Size determined by in vitro translation. (Craig and McCarthy, 1980)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Alpha crystallin/Heat shock protein (IPR001436) Heat shock protein Hsp20 (IPR002068) HSP20-like chaperone (IPR008978)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA536253   AA536255   AE014296 AAF50290   AE014296 AAN11962   AI257387   AI456272   AI520460   AQ073854   AW943326   AX093926   AX154789   AY060412 AAL25451   AY119034 AAM50894   BE978184   BG638118   BP550847   BP551679   CL610262   CO156081   CO182502   CO185064   CO300605   EC085636   EC201226   EC264820   J01098 AAA28635   M13478   M29138   V00216
UniProtKB/Swiss-Prot	P02515
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences	EPD - Eukarytoic Promoter Database, an annotated collection of POL II promoters • 07027
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11   ectoderm | segmentally repeated   (Leemans et al., 2000) northern blot Stage Tissue/Position (including subcellular localization) Reference prepupal stage     (Pauli et al., 1989)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0001223    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0001223 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed during late pupal stages. This gene is annotated by FlyBase as a dicistronic gene, meaning that some or all of its transcripts encode two or more polypeptide-coding open reading frames (ORFs) , with each ORF assigned to a different gene. The distribution of RNA-Seq coverage data amongst the different encoded genes cannot be determined. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0001223 expression level Developmental Stage   Expression Level embryo 00-02hr    1240 embryo 02-04hr    874 embryo 04-06hr    368 embryo 06-08hr    345 embryo 08-10hr    782 embryo 10-12hr    1180 embryo 12-14hr    675 embryo 14-16hr    565 embryo 16-18hr    393 embryo 18-20hr    499 embryo 20-22hr    774 embryo 22-24hr    596 larva L1    280 larva L2    358 larva L3 12hr old    487 larva L3 puffstage 1-2    261 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    607 white prepupae new    970 white prepupae 12hr    5402 white prepupae 24hr    4328 pupae 2d postWPP    15368 pupae 3d postWPP    2050 pupae 4d postWPP    8783 adult male 01day    1278 adult male 05day    1468 adult male 30day    2082 adult female 01day    435 adult female 05day    542 adult female 30day    611 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1240) embryo 02-04hr  (874) embryo 04-06hr  (368) embryo 06-08hr  (345) embryo 08-10hr  (782) embryo 10-12hr  (1180) embryo 12-14hr  (675) embryo 14-16hr  (565) embryo 16-18hr  (393) embryo 18-20hr  (499) embryo 20-22hr  (774) embryo 22-24hr  (596) larva L1  (280) larva L2  (358) larva L3 12hr old  (487) larva L3 puffstage 1-2  (261) larva L3 puffstage 3-6  (336) larva L3 puffstage 7-9  (607) white prepupae new  (970) white prepupae 12hr  (5402) white prepupae 24hr  (4328) pupae 2d postWPP  (15368) pupae 3d postWPP  (2050) pupae 4d postWPP  (8783) adult male 01day  (1278) adult male 05day  (1468) adult male 30day  (2082) adult female 01day  (435) adult female 05day  (542) adult female 30day  (611) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1240 embryo 02-04hr    874 embryo 04-06hr    368 embryo 06-08hr    345 embryo 08-10hr    782 embryo 10-12hr    1180 embryo 12-14hr    675 embryo 14-16hr    565 embryo 16-18hr    393 embryo 18-20hr    499 embryo 20-22hr    774 embryo 22-24hr    596 larva L1    280 larva L2    358 larva L3 12hr old    487 larva L3 puffstage 1-2    261 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    607 white prepupae new    970 white prepupae 12hr  (5402) white prepupae 24hr  (4328) pupae 2d postWPP  (15368) pupae 3d postWPP  (2050) pupae 4d postWPP  (8783) adult male 01day    1278 adult male 05day  1468 adult male 30day  (2082) adult female 01day    435 adult female 05day    542 adult female 30day    611 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1240 embryo 02-04hr    874 embryo 04-06hr    368 embryo 06-08hr    345 embryo 08-10hr    782 embryo 10-12hr    1180 embryo 12-14hr    675 embryo 14-16hr    565 embryo 16-18hr    393 embryo 18-20hr    499 embryo 20-22hr    774 embryo 22-24hr    596 larva L1    280 larva L2    358 larva L3 12hr old    487 larva L3 puffstage 1-2    261 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    607 white prepupae new    970 white prepupae 12hr    5402 white prepupae 24hr    4328 pupae 2d postWPP  (15368) pupae 3d postWPP    2050 pupae 4d postWPP    8783 adult male 01day    1278 adult male 05day    1468 adult male 30day    2082 adult female 01day    435 adult female 05day    542 adult female 30day    611 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1240 embryo 02-04hr    874 embryo 04-06hr    368 embryo 06-08hr    345 embryo 08-10hr    782 embryo 10-12hr    1180 embryo 12-14hr    675 embryo 14-16hr    565 embryo 16-18hr    393 embryo 18-20hr    499 embryo 20-22hr    774 embryo 22-24hr    596 larva L1    280 larva L2    358 larva L3 12hr old    487 larva L3 puffstage 1-2    261 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    607 white prepupae new    970 white prepupae 12hr    5402 white prepupae 24hr    4328 pupae 2d postWPP    15368 pupae 3d postWPP    2050 pupae 4d postWPP    8783 adult male 01day    1278 adult male 05day    1468 adult male 30day    2082 adult female 01day    435 adult female 05day    542 adult female 30day    611 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0001223 expression level Developmental Stage   Expression Level embryo 00-02hr    1240 embryo 02-04hr    874 embryo 04-06hr    368 embryo 06-08hr    345 embryo 08-10hr    782 embryo 10-12hr    1180 embryo 12-14hr    675 embryo 14-16hr    565 embryo 16-18hr    393 embryo 18-20hr    499 embryo 20-22hr    774 embryo 22-24hr    596 larva L1    280 larva L2    358 larva L3 12hr old    487 larva L3 puffstage 1-2    261 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    607 white prepupae new    970 white prepupae 12hr    5402 white prepupae 24hr    4328 pupae 2d postWPP    15368 pupae 3d postWPP    2050 pupae 4d postWPP    8783 adult male 01day    1278 adult male 05day    1468 adult male 30day    2082 adult female 01day    435 adult female 05day    542 adult female 30day    611 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1240) embryo 02-04hr  (874) embryo 04-06hr  (368) embryo 06-08hr  (345) embryo 08-10hr  (782) embryo 10-12hr  (1180) embryo 12-14hr  (675) embryo 14-16hr  (565) embryo 16-18hr  (393) embryo 18-20hr  (499) embryo 20-22hr  (774) embryo 22-24hr  (596) larva L1  (280) larva L2  (358) larva L3 12hr old  (487) larva L3 puffstage 1-2  (261) larva L3 puffstage 3-6  (336) larva L3 puffstage 7-9  (607) white prepupae new  (970) white prepupae 12hr  (5402) white prepupae 24hr  (4328) pupae 2d postWPP  (15368) pupae 3d postWPP  (2050) pupae 4d postWPP  (8783) adult male 01day  (1278) adult male 05day  (1468) adult male 30day  (2082) adult female 01day  (435) adult female 05day  (542) adult female 30day  (611) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1240 embryo 02-04hr    874 embryo 04-06hr    368 embryo 06-08hr    345 embryo 08-10hr    782 embryo 10-12hr    1180 embryo 12-14hr    675 embryo 14-16hr    565 embryo 16-18hr    393 embryo 18-20hr    499 embryo 20-22hr    774 embryo 22-24hr    596 larva L1    280 larva L2    358 larva L3 12hr old    487 larva L3 puffstage 1-2    261 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    607 white prepupae new    970 white prepupae 12hr  (5402) white prepupae 24hr  (4328) pupae 2d postWPP  (15368) pupae 3d postWPP  2050 pupae 4d postWPP  (8783) adult male 01day    1278 adult male 05day  1468 adult male 30day  2082 adult female 01day    435 adult female 05day    542 adult female 30day    611 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1240 embryo 02-04hr    874 embryo 04-06hr    368 embryo 06-08hr    345 embryo 08-10hr    782 embryo 10-12hr    1180 embryo 12-14hr    675 embryo 14-16hr    565 embryo 16-18hr    393 embryo 18-20hr    499 embryo 20-22hr    774 embryo 22-24hr    596 larva L1    280 larva L2    358 larva L3 12hr old    487 larva L3 puffstage 1-2    261 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    607 white prepupae new    970 white prepupae 12hr    5402 white prepupae 24hr    4328 pupae 2d postWPP  15368 pupae 3d postWPP    2050 pupae 4d postWPP    8783 adult male 01day    1278 adult male 05day    1468 adult male 30day    2082 adult female 01day    435 adult female 05day    542 adult female 30day    611 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1240 embryo 02-04hr    874 embryo 04-06hr    368 embryo 06-08hr    345 embryo 08-10hr    782 embryo 10-12hr    1180 embryo 12-14hr    675 embryo 14-16hr    565 embryo 16-18hr    393 embryo 18-20hr    499 embryo 20-22hr    774 embryo 22-24hr    596 larva L1    280 larva L2    358 larva L3 12hr old    487 larva L3 puffstage 1-2    261 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    607 white prepupae new    970 white prepupae 12hr    5402 white prepupae 24hr    4328 pupae 2d postWPP    15368 pupae 3d postWPP    2050 pupae 4d postWPP    8783 adult male 01day    1278 adult male 05day    1468 adult male 30day    2082 adult female 01day    435 adult female 05day    542 adult female 30day    611 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0001223    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0001223 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: larval/adult Malpighian tubules, larval salivary gland, adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, adult crop, larval hindgut, adult heart, larval fat body, adult ovary, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Heatmap Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut     Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye no informative data Adult Brain no informative data Adult Thoracic-Abdominal Ganglion     Adult Crop no informative data Adult Midgut     Adult Hindgut     Adult Malpighian Tubules no informative data Adult Fat Body     Adult Salivary Gland     Adult Heart no informative data Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary no informative data Adult Testis no informative data Adult Male Accessory Gland     Adult Carcass no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_27
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001223
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Hsp22EY09909 Sterility Allele fertile Hsp22EY09909 Other Phenotypes Allele cold stress response defective, with Scer\GAL4Act5C.PI Hsp22GD8704 locomotor behavior defective Hsp22EP3583-11 long lived, with Scer\GAL4Act5C.PI Hsp22EP3247 short lived Hsp22EP3583-11 Phenotype manifest in Allele
Classical Alleles ( 10 )
For All Classical Alleles Show
Allele of Hsp22 Class Mutagen Stocks Known lesion Hsp22BG02409 1 Yes Hsp22c06661 1 -- Hsp22d00797 1 -- Hsp22d07618 1 -- Hsp22EY09909 P-element activity 1 Yes Hsp22NP2055 1 -- Hsp22NP5403 1 -- Hsp22d07456 0 -- Hsp22EP3247 P-element activity 0 Yes Hsp22EP3583-11 0 Yes
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Hsp22 Class Mutagen Stocks Known lesion Hsp22GD8704 1 -- Hsp22T6BV1 in vitro construct 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data reporter construct P{Hsp22-lacZ.5'Δ-114} No P{Hsp22-lacZ.5'Δ-314.mut} No P{Hsp22-lacZ.5'Δ-314} No heat-shock construct P{GD8704} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}EP3583-10 NA P{EP}EP3583-12 NA P{EP}EP3583-18 NA P{EP}Hsp22EP3583-11 NA P{EP}Hsp67BbEP3247 NA P{EPgy2}Hsp22EY09909 NA P{XP}Hsp22d00797 NA P{XP}Hsp22d07456 NA P{XP}Hsp22d07618 NA miscellaneous insertions P{GT1}Hsp22BG02409 NA PBac{PB}Hsp22c06661 NA insertion of enhancer trap binary system P{GawB}Hsp22NP2055 No P{GawB}Hsp22NP5403 No
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
determination of adult lifespan inferred from mutant phenotype (Morrow et al., 2004, Morrow et al., 2004) response to heat inferred from mutant phenotype (Morrow et al., 2004) response to oxidative stress inferred from mutant phenotype (Morrow et al., 2004)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
protein refolding non-traceable author statement (Swiss-Prot Project Members, 1986.7.21) response to heat inferred from sequence or structural similarity with EMBL:U96099 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1986.7.21)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene_with_dicistronic_mRNA (FlyBase Genome Annotators, 2002-2003) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Hsp22 allele Gene References Hsp22EP3247 pnr (Pena-Rangel et al., 2002)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 534173 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0001223 DroID - A comprehensive database of gene and protein interactions. • FBgn0001223 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 3772576
Orthologs
Genome-wide drosophilid orthologs	Dana\GF10689 Dere\GG15367 Dgri\GH14586 Dmoj\GI12244 Dper\GL22441 Dpse\GA16631 Dsec\GM25140 Dsim\GD14174 Dvir\GJ11476 Dwil\GK17635 Dyak\GE20830
Curated drosophilid orthologs	Damb\Hsp22   Daur\Hsp22   Dbio\Hsp22   Dbir\Hsp22   Ddip\Hsp22   Dgua\Hsp22   Djam\Hsp22   Dkik\Hsp22   Dmad\Hsp22   Dpse\GA16631   Dqua\Hsp22   Drep\Hsp22   Dseg\Hsp22   Dser\Hsp22   Dsub\Hsp22   Dtra\Hsp22   Dvul\Hsp22
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • At1g07400.1 Arabidopsis thaliana (wall cress) At5g12030.1 Arabidopsis thaliana (wall cress) 151636 Branchiostoma floridae (lancelet) 151683 Branchiostoma floridae (lancelet) 237499 Branchiostoma floridae (lancelet) 252489 Branchiostoma floridae (lancelet) LOC_Os01g04340.1 Oryza sativa (asian rice) LOC_Os03g15960.1 Oryza sativa (asian rice) LOC_Os11g13980.1 Oryza sativa (asian rice) 113859 Physcomitrella patens (moss) 158998 Physcomitrella patens (moss) 216427 Physcomitrella patens (moss) 65913 Physcomitrella patens (moss) 650093 Populus trichocarpa (black cottonwood) 722968 Populus trichocarpa (black cottonwood) 832078 Populus trichocarpa (black cottonwood) 148058 Sorghum bicolor (sorghum) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0238121 FBgn0219634 FBgn0198734 FBgn0185861 FBgn0180001 FBgn0076646 FBgn0160033 FBgn0135001 FBgn0122062 FBgn0122033 FBgn0107619 FBgn0087730 GLEAN_05529 GLEAN_05528
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	12683 w1118; P{GT1}Hsp22BG02409 Hsp67BbBG02409 20055 y1 w67c23; P{EPgy2}Hsp22EY09909 Hsp67BbEY09909
Harvard	- P{XP}Hsp22d00797 - P{XP}Hsp22d07618 - PBac{PB}Hsp22c06661
Kyoto	113720 w*; P{GawB}Hsp22NP5403 / TM6, P{UAS-lacZ.UW23-1}UW23-1 104072 w*; P{GawB}Hsp22NP2055 / TM3, Sb1 Ser1
VDRC	v43632 w1118; P{GD8704}v43632
Genomic Clones ( 1 )
	BACR01E20 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 16 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD36162 LP05734
Other clones	DPiM_LD36162
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD16753 LD40634
Other clones	57630 73207 527120 bs75b08 EC22858 EC29258 EK178661 EN07317 FGM209E07 FGM214B11 LD16746
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0001223 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 317836 3772576
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments	Dicistronic annotation CG32041 split out into separate annotations for each open reading frame, CG4456 and CG4460, in release 4.2 of the genome annotation. CG4456 corresponds to Hsp67Bb and CG4460 corresponds to Hsp22. (FlyBase Genome Annotators, 2005) One or more of the processed transcripts for this gene contain(s) two non-overlapping open reading frames (ORFs). The non-overlapping ORFs are represented by Hsp67Bb and Hsp22. (FlyBase Genome Annotators, 2002-2003)
Other Comments
	Expression is rapidly induced in the testis after heat stress. Activation of Hsp22 in testes and heads necessitates the presence of a functional Hsf. (Michaud et al., 1997) Increase in RNA levels is observed with aging. (Wheeler et al., 1995) Chromosome staining reveals that Trl and heat shock transcription factors (HSF) colocalises at Hsp22. (Tsukiyama et al., 1994) In vivo UV cross-linking and nuclear run-on assays shows that RNA polymerase II density on the Hsp22 gene is rapidly increased by heat shock. (O'Brien and Lis, 1993) In unshocked cells Hsp83 is moderately transcribed while transcription from the other heat shock genes is undetectable. Engaged but paused RNA molecules are found at the various Hsp70 and Hsp26 genes but not at the other heat shock genes. Increased transcription of the heat shock genes is observed within 1-2 mins of heat shock and maximal rates were reached within 2-5 minutes. Rates of transcription vary over a 20-fold range. (Vazquez et al., 1993) Exposure of cells to pulses of elevated temperature initiates the heat-shock response. A restricted subset of genes, the Hsp genes, is activated and the majority of transcription and translation is shut down. 3H-uridine incorporation ceases at its usual positions and commences at new puff sites. Preexisting polysomes disaggregate and within a few minutes a new population of polysomes appears containing newly transcribed mRNA; this RNA hybridizes to some of the heat-shock puffs. Similar response inducible by other stressful treatments. The response may be elicited at all stages of the life cycle and in cultured cells. (Lindsley and Zimm, 1992) Hsp22 regulatory regions involved in heat shock expression and ecdysterone-induced expression have been identified. (Rudolph et al., 1991) Translation of Hsp70 mRNAs and to a lesser extent the mRNAs for the small heat shock proteins is almost independent of eIF-4E. (Zapata et al., 1991) Mutations at br reduce the transcription rate or stability of the small heat shock protein mRNAs. (Dubrovsky et al., 1990) Heat shock induces the accumulation of a dicistronic mRNA which contains both the Hsp22 and Hsp67Bb open reading frames. Overall orientation not stated: Hsp27- Hsp23- Hsp67Ba+ Hsp26+ Hsp22- Hsp67Bb- Hsp67Bc+ (Pauli et al., 1988) Heat-shock inducible in almost all cells at the stages tested. (Ayme-Southgate and Tissieres, 1985) Activation of transcription of Hsp genes apparently involves the sequential binding of two or more protein factors in vicinity of TATA box. (Wu, 1984) Polymerase II dissociates from most chromosome regions and accumulates at the new heat shock puff sites upon heat shock. (Bonner and Kerby, 1982) The binding sites for the protein factors required for activation of transcription of Hsp genes are multiple short upstream sequence elements called HSEs or heat shock consensus elements. (Pelham, 1982, Xiao and Lis, 1988) Hsp22 is transcribed during certain developmental stages in the absence of heat shock. (Sirotkin and Davidson, 1982) The effects of heat shock may be abrogated to some degree by pretreatment with a pulse of a slightly lower temperature. (Mitchell et al., 1979, Petersen and Mitchell, 1981) Mitochondrial and histone-gene activities persist transcription and translation. (Spradling et al., 1977) In polytene cells, during heat shock response, existing puffs regress and a novel group quickly appear at 33B, 63C, 64F, 67B, 70A, 87A, 87C, 93D, 95D. (Tissieres et al., 1974) The heat shock response follows a pulse of 36oC to 40oC; treatments above 40oC inhibit all activity and lead to death; treatments of 30oC-35oC induce heat-shock-protein synthesis without repressing normal protein synthesis. (Tissieres et al., 1974) In polytene cells, during heat shock response, existing puffs regress and a novel group quickly appear at cytological locations 33B, 63C, 64F, 67B, 70A, 87A, 87C, 93D, 95D. (Ashburner, 1970)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_001031943 NM_001031944 RefSeq (Proteins) • NP_001027114 NP_001027115 DDBJ / EMBL / GenBank • AAA28635 AAF50290 AAF50290.1 AAF50291.1 AAL25451 AAM50894 AAN11961.1 AAN11962 AAN11962.1 AI257387 AI456272 AQ073854 AW943326 AX093926 AX154789 AY060412 AY119034 CL610262 J01098 M13478 M29138 V00216 Entrez Gene - A searchable database of RefSeq genes. • 3772576 UniProtKB/Swiss-Prot • P02515
Other Crossreferences
	EPD - Eukarytoic Promoter Database, an annotated collection of POL II promoters • 07027 InterPro domains - A database of protein families, domains, and functional sites • Alpha crystallin/Heat shock protein (IPR001436) Heat shock protein Hsp20 (IPR002068) HSP20-like chaperone (IPR008978)
Linkouts
	BioGRID - A database of protein and genetic interactions • 534173 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0001223 DroID - A comprehensive database of gene and protein interactions. • FBgn0001223 DRSC - Results from RNAi screens. • FBgn0001223 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001223 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0001223 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 317836 3772576 InParanoid A subset of ortholog calls from InParanoid. • At1g07400.1 Arabidopsis thaliana (wall cress) At5g12030.1 Arabidopsis thaliana (wall cress) 151636 Branchiostoma floridae (lancelet) 151683 Branchiostoma floridae (lancelet) 237499 Branchiostoma floridae (lancelet) 252489 Branchiostoma floridae (lancelet) LOC_Os01g04340.1 Oryza sativa (asian rice) LOC_Os03g15960.1 Oryza sativa (asian rice) LOC_Os11g13980.1 Oryza sativa (asian rice) 113859 Physcomitrella patens (moss) 158998 Physcomitrella patens (moss) 216427 Physcomitrella patens (moss) 65913 Physcomitrella patens (moss) 650093 Populus trichocarpa (black cottonwood) 722968 Populus trichocarpa (black cottonwood) 832078 Populus trichocarpa (black cottonwood) 148058 Sorghum bicolor (sorghum) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 3772576 modMine - Data generated by the modENCODE project. • FBgn0001223 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0238121 FBgn0219634 FBgn0198734 FBgn0185861 FBgn0180001 FBgn0076646 FBgn0160033 FBgn0135001 FBgn0122062 FBgn0122033 FBgn0107619 FBgn0087730 GLEAN_05529 GLEAN_05528
Synonyms & Secondary IDs ( 14 )
Reported As
Symbol Synonym	22 (Petersen and Lindquist, 1988) anon-WO0118547.50   anon-WO0140519.138   CG4460   CG32041 (Giot et al., 2003, Lai et al., 2007, Birch-Machin et al., 2005, Kim et al., 2010) DmHsp22 (Morrow et al., 2000, Wadhwa et al., 2010) EP(3)3247 (Pena-Rangel et al., 2002) Hsp22 (Sudi et al., 2008, Geiger-Thornsberry and Mackay, 2004, Gruenewald et al., 2009, Morettini et al., 2011, Yepiskoposyan et al., 2006, Walser et al., 2006, Harvey et al., 2008, Takahashi et al., 2011, Gilchrist et al., 2008, Bettencourt et al., 2008, Morrow et al., 2004, Gim et al., 2001, Fernández-Ayala et al., 2010, Tian et al., 2010, Xi et al., 2008, Takahashi et al., 2010, Wadhwa et al., 2010, Colinet et al., 2010, Lorbeck et al., 2010, Kim et al., 2010, Neal et al., 2006, Duncan, 2005, Colinet et al., 2010, Groebe et al., 2010, Kim et al., 2010) hsp22 (Landis et al., 2004, Morrow et al., 2004, Kurapati et al., 2000, Aigaki et al., 2002, Bhole et al., 2001, Ayme-Southgate, 1993.2.23, Tatar, 1999, King and Tower, 1999, Otsuka et al., 1997, Shopland and Lis, 1996, Wheeler et al., 1995, Champlin and Lis, 1994, O'Brien and Lis, 1993, Molto et al., 1993, Heikkila, 1993, Vazquez et al., 1993, Pauli et al., 1992, Berger et al., 1992, Vazquez, 1991, Dobens et al., 1991, Zapata et al., 1991, Rudolph et al., 1991, Bendena et al., 1991, Yost et al., 1990, Ornelles and Penman, 1990, Thummel, 1990, Pauli et al., 1989, Pauli et al., 1988, Pauli and Tonka, 1987, Ayme-Southgate and Tissieres, 1985, Southgate et al., 1983, Ingolia and Craig, 1981, Bhole et al., 2004, Zhao et al., 2005, Hull-Thompson et al., 2009, Kurapati et al., 2004, Grover et al., 2009, Zhao et al., 2005, Yang and Tower, 2009, Morrow et al., 2004, Curtis et al., 2007, Chan et al., 2011, Sharma et al., 2011) small hsp locus 67B (Yao et al., 2007)
Name Synonym	Heat shock protein 22 (Gim et al., 2001, Kim et al., 2010) heat shock protein 22 (Lorbeck et al., 2010) heat shock protein hsp22 (Ingolia, 1985.2.22) Protein 22 (Bournias-Vardiabasis et al., 1990)
Secondary FlyBase IDs
	FBgn0044743 FBgn0052041 FBgn0061943 FBpp0076176 FBpp0076177 FBpp0076178 FBpp0076179 FBtr0076448 FBtr0076449 FBtr0076450 FBtr0076451
References ( 141 )
Generate a list of	All references relating to this gene ( 141 )
List References by type	Research paper  ( 112 ) Supplementary material  ( 2 ) Review  ( 9 ) Abstract  ( 2 ) FlyBase analysis  ( 5 ) DNA/RNA sequence record  ( 2 ) Patent  ( 2 ) Book  ( 1 ) Protein sequence record  ( 1 ) Computer file  ( 3 ) Curated genome annotation  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers ( 15 )
	Chan et al., 2011, Hum. Mol. Genet. 20(9): 1738--1750 Expanded polyglutamine domain possesses nuclear export activity which modulates subcellular localization and toxicity of polyQ disease protein via exportin-1. [FBrf0214367] Morettini et al., 2011, Nucleic Acids Res. 39(8): 3103--3115 The chromodomains of CHD1 are critical for enzymatic activity but less important for chromatin localization. [FBrf0213515] Sharma et al., 2011, Chemosphere 82(3): 370--376 Transcriptome analysis provides insights for understanding the adverse effects of endosulfan in Drosophila melanogaster. [FBrf0212610] Takahashi et al., 2011, PLoS ONE 6(4): e17295 Environmental Stress-Dependent Effects of Deletions Encompassing Hsp70Ba on Canalization and Quantitative Trait Asymmetry in Drosophila melanogaster. [FBrf0213649] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Colinet et al., 2010, FEBS J. 277(1): 174--185 Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster. [FBrf0209693] Colinet et al., 2010, J. Exp. Biol. 213(24): 4146--4150 Knocking down expression of Hsp22 and Hsp23 by RNA interference affects recovery from chill coma in Drosophila melanogaster. [FBrf0212357] Fernández-Ayala et al., 2010, PLoS ONE 5(1): e8549 Gene expression in a Drosophila model of mitochondrial disease. [FBrf0209705] Groebe et al., 2010, Exp. Gerontol. 45(7-8): 632--637 Age-dependent posttranslational modifications of voltage-dependent anion channel 1. [FBrf0210871] Kim et al., 2010, Exp. Gerontol. 45(7-8): 611--620 Gene expression profiling implicates OXPHOS complexes in lifespan extension of flies over-expressing a small mitochondrial chaperone, Hsp22. [FBrf0210880] Kim et al., 2010, Exp. Gerontol. 45(12): 984--987 Enhancement of stress resistances and downregulation of Imd pathway by lower developmental temperature in Drosophila melanogaster. [FBrf0212401] Lorbeck et al., 2010, Gene 450(1-2): 8--17 The histone demethylase Dmel\Kdm4A controls genes required for life span and male-specific sex determination in Drosophila. [FBrf0209540] Takahashi et al., 2010, BMC Evol. Biol. 10: 284 Effects of small Hsp genes on developmental stability and microenvironmental canalization. [FBrf0211941] Tian et al., 2010, PLoS ONE 5(5): e10669 Phylogeny Disambiguates the Evolution of Heat-Shock cis-Regulatory Elements in Drosophila. [FBrf0210820] Wadhwa et al., 2010, J. Biol. Chem. 285(6): 3833--3839 Proproliferative functions of Drosophila small mitochondrial heat shock protein 22 in human cells. [FBrf0209839] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...