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Gene Dmel\Hsp67Bb

General Information
Symbol	Dmel\Hsp67Bb	Species	D. melanogaster
Name	Heat shock gene 67Bb	Annotation symbol	CG4456
Feature type	protein_coding_gene	FlyBase ID	FBgn0001228
Gene Model Status	Current	Stock availability	2 publicly available
Also Known As	gene 2, CG32041
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	67B2-67B2	Sequence location	3L:9,365,822..9,368,064 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Heat shock gene 67Bb is referred to in FlyBase by the symbol Dmel\Hsp67Bb (CG4456, FBgn0001228). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. It is reported to be involved in the biological process: response to heat. 3 alleles are reported. No phenotypic data is available. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Rhodanese-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed during late pupal stages. This gene is annotated by FlyBase as a dicistronic gene, meaning that some or all of its transcripts encode two or more polypeptide-coding open reading frames (ORFs) , with each ORF assigned to a different gene. The distribution of RNA-Seq coverage data amongst the different encoded genes cannot be determined. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: larval/adult Malpighian tubules, larval salivary gland, adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, adult crop, larval hindgut, adult heart, larval fat body, adult ovary, adult carcass. Gene sequence location is 3L:9365822..9368064.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Hsp22 - Hsp-G3 There are seven closely related heat-shock genes at 67B (Ayme and Tissieres, 1985; Pauli, Arrigo, Vasquez, Tonka, and Tissieres, 1989, Genome 31: 671-76). In addition to the four small heat-shock genes previously identified (Hsp22, Hsp23, Hsp26, and Hsp27), three more genes (Hsp-G1, Hsp-G2, and Hsp-G3, formerly called Gene1, Gene2, and Gene3) have been found clustered within 15 kb of DNA at the same 67B cytological location. All seven genes are heat-shock inducible in almost all cells at the stages tested (Ayme and Tissieres, 1985). The genes are also transcribed during certain developmental stages in the absence of heat shock (Sirotkin and Davidson, 1982, Dev. Biol. 89: 196-210). Pauli et al (1989) report that the maximum accumulation of developmental rRNA in a majority of these small heat-shock genes occurs in the white pupae stage; in Hsp-G2, however, a small transcipt is found in embryos, first and second instar larvae, and young pupae; and a larger transcript in the pupal and adult stages of males (Pauli and Tonka, 1987, J. Mol. Biol. 198: 235-40; Pauli, Tonka, and Ayme-Southgate, 1988, J. Mol. Biol. 200: 47-53). In absence of stress, the expression of Hsp26 has been observed in spermatocytes, nurse cells, epithelium, imaginal discs, proventriculus, and neurocytes (Glaser, Wolfner, and Lis, 1986, EMBO 5: 747-54). Transcripts of Hsp26 and Hsp27 accumulate in adult ovaries, apparently originating in nurse cells (Zimmerman, Petri, and Meselson, 1983, Cell 32: 1161-70).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10
FB2012_01	References Mosqueira et al., 2010, PLoS ONE 5(10): e13450
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 67B2-67B2   Limits computationally determined from genome sequence between P{PZ}l(3)0162901629&P{PZ}mRpL1210534 and P{EP}Hsp26EP3336&P{EP}Hsp26EP3315
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Hsp67Bb for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	gene_with_dicistronic_mRNA ; SO:0000722
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Hsp67Bb-RA FBtr0100653  NM_001031946   585   111 Hsp67Bb-RB FBtr0100655  NM_001031945   1900   111 Hsp67Bb-RC FBtr0303473  NM_001202147   724   111 Hsp67Bb-RD FBtr0303474  NM_001202148   794   111
Additional Transcript Data & Comments
Reported size (kB)	2, 0.78, 0.56 (northern blot) (Pauli et al., 1988)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Hsp67Bb-PA   FBpp0100114   12.6   111   8.43     NP_001027117   AAN11961 Hsp67Bb-PB   FBpp0100115   12.6   111   8.43     NP_001027116   AAF50291 Hsp67Bb-PC   FBpp0292525   12.6   111   8.43     NP_001189076   ADV37513 Hsp67Bb-PD   FBpp0292526   12.6   111   8.43     NP_001189077   ADV37514
Additional Polypeptide Data & Comments
Reported size (kDa)	111 (aa) (Pauli et al., 1988)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Rhodanese-like (IPR001763)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA536253   AA536255   AE014296 AAF50291   AE014296 AAN11961   AE014296 ADV37513   AE014296 ADV37514   AI456272   AI520460   AQ073854   AW943326   AX093926   AX154789   AY119034   BE978184   BG638118   BP550847   BP551679   CL610262   CO156081   CO182502   CO185064   CO293233   CO300605   EC085636   EC201226   EC264820   X07311 CAA30276
UniProtKB/Swiss-Prot	P22978
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11 -- 17     (Pauli et al., 1988) Comment:reference states >=7 hr AEL first instar larval stage     (Pauli et al., 1988) second instar larval stage     (Pauli et al., 1988) third instar larval stage     (Pauli et al., 1988) pupal stage     (Pauli et al., 1988) pupal stage | early     (Pauli et al., 1988) adult stage | male     (Pauli et al., 1988) adult stage     (Pauli et al., 1988) spermatogenesis & adult stage   testis   (Pauli et al., 1988)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0001228    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0001228 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed during late pupal stages. This gene is annotated by FlyBase as a dicistronic gene, meaning that some or all of its transcripts encode two or more polypeptide-coding open reading frames (ORFs) , with each ORF assigned to a different gene. The distribution of RNA-Seq coverage data amongst the different encoded genes cannot be determined. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0001228 expression level Developmental Stage   Expression Level embryo 00-02hr    1240 embryo 02-04hr    874 embryo 04-06hr    368 embryo 06-08hr    345 embryo 08-10hr    782 embryo 10-12hr    1180 embryo 12-14hr    675 embryo 14-16hr    565 embryo 16-18hr    393 embryo 18-20hr    499 embryo 20-22hr    774 embryo 22-24hr    596 larva L1    280 larva L2    358 larva L3 12hr old    487 larva L3 puffstage 1-2    261 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    607 white prepupae new    970 white prepupae 12hr    5402 white prepupae 24hr    4328 pupae 2d postWPP    15368 pupae 3d postWPP    2050 pupae 4d postWPP    8783 adult male 01day    1278 adult male 05day    1468 adult male 30day    2082 adult female 01day    435 adult female 05day    542 adult female 30day    611 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1240) embryo 02-04hr  (874) embryo 04-06hr  (368) embryo 06-08hr  (345) embryo 08-10hr  (782) embryo 10-12hr  (1180) embryo 12-14hr  (675) embryo 14-16hr  (565) embryo 16-18hr  (393) embryo 18-20hr  (499) embryo 20-22hr  (774) embryo 22-24hr  (596) larva L1  (280) larva L2  (358) larva L3 12hr old  (487) larva L3 puffstage 1-2  (261) larva L3 puffstage 3-6  (336) larva L3 puffstage 7-9  (607) white prepupae new  (970) white prepupae 12hr  (5402) white prepupae 24hr  (4328) pupae 2d postWPP  (15368) pupae 3d postWPP  (2050) pupae 4d postWPP  (8783) adult male 01day  (1278) adult male 05day  (1468) adult male 30day  (2082) adult female 01day  (435) adult female 05day  (542) adult female 30day  (611) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1240 embryo 02-04hr    874 embryo 04-06hr    368 embryo 06-08hr    345 embryo 08-10hr    782 embryo 10-12hr    1180 embryo 12-14hr    675 embryo 14-16hr    565 embryo 16-18hr    393 embryo 18-20hr    499 embryo 20-22hr    774 embryo 22-24hr    596 larva L1    280 larva L2    358 larva L3 12hr old    487 larva L3 puffstage 1-2    261 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    607 white prepupae new    970 white prepupae 12hr  (5402) white prepupae 24hr  (4328) pupae 2d postWPP  (15368) pupae 3d postWPP  (2050) pupae 4d postWPP  (8783) adult male 01day    1278 adult male 05day  1468 adult male 30day  (2082) adult female 01day    435 adult female 05day    542 adult female 30day    611 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1240 embryo 02-04hr    874 embryo 04-06hr    368 embryo 06-08hr    345 embryo 08-10hr    782 embryo 10-12hr    1180 embryo 12-14hr    675 embryo 14-16hr    565 embryo 16-18hr    393 embryo 18-20hr    499 embryo 20-22hr    774 embryo 22-24hr    596 larva L1    280 larva L2    358 larva L3 12hr old    487 larva L3 puffstage 1-2    261 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    607 white prepupae new    970 white prepupae 12hr    5402 white prepupae 24hr    4328 pupae 2d postWPP  (15368) pupae 3d postWPP    2050 pupae 4d postWPP    8783 adult male 01day    1278 adult male 05day    1468 adult male 30day    2082 adult female 01day    435 adult female 05day    542 adult female 30day    611 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1240 embryo 02-04hr    874 embryo 04-06hr    368 embryo 06-08hr    345 embryo 08-10hr    782 embryo 10-12hr    1180 embryo 12-14hr    675 embryo 14-16hr    565 embryo 16-18hr    393 embryo 18-20hr    499 embryo 20-22hr    774 embryo 22-24hr    596 larva L1    280 larva L2    358 larva L3 12hr old    487 larva L3 puffstage 1-2    261 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    607 white prepupae new    970 white prepupae 12hr    5402 white prepupae 24hr    4328 pupae 2d postWPP    15368 pupae 3d postWPP    2050 pupae 4d postWPP    8783 adult male 01day    1278 adult male 05day    1468 adult male 30day    2082 adult female 01day    435 adult female 05day    542 adult female 30day    611 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0001228 expression level Developmental Stage   Expression Level embryo 00-02hr    1240 embryo 02-04hr    874 embryo 04-06hr    368 embryo 06-08hr    345 embryo 08-10hr    782 embryo 10-12hr    1180 embryo 12-14hr    675 embryo 14-16hr    565 embryo 16-18hr    393 embryo 18-20hr    499 embryo 20-22hr    774 embryo 22-24hr    596 larva L1    280 larva L2    358 larva L3 12hr old    487 larva L3 puffstage 1-2    261 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    607 white prepupae new    970 white prepupae 12hr    5402 white prepupae 24hr    4328 pupae 2d postWPP    15368 pupae 3d postWPP    2050 pupae 4d postWPP    8783 adult male 01day    1278 adult male 05day    1468 adult male 30day    2082 adult female 01day    435 adult female 05day    542 adult female 30day    611 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1240) embryo 02-04hr  (874) embryo 04-06hr  (368) embryo 06-08hr  (345) embryo 08-10hr  (782) embryo 10-12hr  (1180) embryo 12-14hr  (675) embryo 14-16hr  (565) embryo 16-18hr  (393) embryo 18-20hr  (499) embryo 20-22hr  (774) embryo 22-24hr  (596) larva L1  (280) larva L2  (358) larva L3 12hr old  (487) larva L3 puffstage 1-2  (261) larva L3 puffstage 3-6  (336) larva L3 puffstage 7-9  (607) white prepupae new  (970) white prepupae 12hr  (5402) white prepupae 24hr  (4328) pupae 2d postWPP  (15368) pupae 3d postWPP  (2050) pupae 4d postWPP  (8783) adult male 01day  (1278) adult male 05day  (1468) adult male 30day  (2082) adult female 01day  (435) adult female 05day  (542) adult female 30day  (611) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1240 embryo 02-04hr    874 embryo 04-06hr    368 embryo 06-08hr    345 embryo 08-10hr    782 embryo 10-12hr    1180 embryo 12-14hr    675 embryo 14-16hr    565 embryo 16-18hr    393 embryo 18-20hr    499 embryo 20-22hr    774 embryo 22-24hr    596 larva L1    280 larva L2    358 larva L3 12hr old    487 larva L3 puffstage 1-2    261 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    607 white prepupae new    970 white prepupae 12hr  (5402) white prepupae 24hr  (4328) pupae 2d postWPP  (15368) pupae 3d postWPP  2050 pupae 4d postWPP  (8783) adult male 01day    1278 adult male 05day  1468 adult male 30day  2082 adult female 01day    435 adult female 05day    542 adult female 30day    611 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1240 embryo 02-04hr    874 embryo 04-06hr    368 embryo 06-08hr    345 embryo 08-10hr    782 embryo 10-12hr    1180 embryo 12-14hr    675 embryo 14-16hr    565 embryo 16-18hr    393 embryo 18-20hr    499 embryo 20-22hr    774 embryo 22-24hr    596 larva L1    280 larva L2    358 larva L3 12hr old    487 larva L3 puffstage 1-2    261 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    607 white prepupae new    970 white prepupae 12hr    5402 white prepupae 24hr    4328 pupae 2d postWPP  15368 pupae 3d postWPP    2050 pupae 4d postWPP    8783 adult male 01day    1278 adult male 05day    1468 adult male 30day    2082 adult female 01day    435 adult female 05day    542 adult female 30day    611 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1240 embryo 02-04hr    874 embryo 04-06hr    368 embryo 06-08hr    345 embryo 08-10hr    782 embryo 10-12hr    1180 embryo 12-14hr    675 embryo 14-16hr    565 embryo 16-18hr    393 embryo 18-20hr    499 embryo 20-22hr    774 embryo 22-24hr    596 larva L1    280 larva L2    358 larva L3 12hr old    487 larva L3 puffstage 1-2    261 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    607 white prepupae new    970 white prepupae 12hr    5402 white prepupae 24hr    4328 pupae 2d postWPP    15368 pupae 3d postWPP    2050 pupae 4d postWPP    8783 adult male 01day    1278 adult male 05day    1468 adult male 30day    2082 adult female 01day    435 adult female 05day    542 adult female 30day    611 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0001228    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0001228 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: larval/adult Malpighian tubules, larval salivary gland, adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, adult crop, larval hindgut, adult heart, larval fat body, adult ovary, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Heatmap Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut     Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye no informative data Adult Brain no informative data Adult Thoracic-Abdominal Ganglion     Adult Crop no informative data Adult Midgut     Adult Hindgut     Adult Malpighian Tubules no informative data Adult Fat Body     Adult Salivary Gland     Adult Heart no informative data Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary no informative data Adult Testis no informative data Adult Male Accessory Gland     Adult Carcass no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001228 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32041-RB;CG32041-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Hsp67BbEY09909 Sterility Allele fertile Hsp67BbEY09909 Phenotype manifest in Allele
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Hsp67Bb Class Mutagen Stocks Known lesion Hsp67BbBG02409 1 Yes Hsp67BbEY09909 P-element activity 1 Yes Hsp67BbEP3247 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 0 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Hsp67Bb Class Mutagen Stocks Known lesion
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD8704} NA P{GD17696} NA P{GD17697} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}Hsp67BbEP3247 NA P{EPgy2}Hsp22EY09909 NA miscellaneous insertions P{GT1}Hsp22BG02409 NA
Gene Ontology: Function, Process & Cellular Component ( 1 unique term )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
response to heat non-traceable author statement (FlyBase, 1992-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene_with_dicistronic_mRNA (FlyBase Genome Annotators, 2002-2003) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Hsp67Bb allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0001228 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 3771872
Orthologs
Genome-wide drosophilid orthologs	Dana\GF10688 Dere\GG15366 Dgri\GH14585 Dmoj\GI12243 Dper\GL22440 Dpse\GA24291 Dsec\GM25139 Dsim\GD14173 Dvir\GJ11475 Dwil\GK17634 Dyak\Hsp67Bb
Curated drosophilid orthologs	Dyak\Hsp67Bb
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • 83482 Branchiostoma floridae (lancelet) ENSCAFP00000018657 Canis familiaris (dog) 144137 Chlamidomonas reinhardtii (green alga) 92990 Daphnia pulex (daphnia) ENSECAP00000021813 Equus caballus (horse) ENSGALP00000024896 Gallus gallus (chicken) ISCW023160-PA Ixodes scapularis (deer tick) LmjF09.1470 Leishmania major (virus) 9436 Monocosta brevicollis (choanoflagellate) 14863 Ostreococcus tauri (green alga) YOR285W Saccharomyces cerevisiae (budding yeast) YOR286W Saccharomyces cerevisiae (budding yeast) Smp_007450.2 Schistosoma mansoni (trematode parasite) Tc00.1047053506753.130 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053510357.20 Trypanosoma cruzi (parasitic trypanosome) 260962 Thalassiosira pseudonana (marine diatom) 39141 Thalassiosira pseudonana (marine diatom) YALI0B01650g Yarrowia lipolytica (nonconventional yeast) YALI0F29667g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB17694 NV15588 LH19518 ISCW023160 ISCW015859 FBgn0243252 FBgn0240626 FBgn0068141 FBgn0219633 FBgn0215740 FBgn0213162 FBgn0201616 FBgn0201410 FBgn0198733 FBgn0192515 FBgn0190371 FBgn0185860 FBgn0181407 FBgn0180000 FBgn0178927 JGI_V11_92990 JGI_V11_219795 JGI_V11_336777 FBgn0248612 FBgn0245688 FBgn0071580 FBgn0162014 FBgn0160987 FBgn0160032 FBgn0147355 FBgn0135000 FBgn0133568 FBgn0039145 FBgn0038080 FBgn0132701 FBgn0126063 FBgn0125955 FBgn0122061 FBgn0111420 FBgn0107618 FBgn0103551 FBgn0097135 FBgn0094138 FBgn0094056 FBgn0087729 CPIJ013882 CPIJ013880 CPIJ013881 BGIBMGA002501 ACYPI008513 GB17201 AGAP013228 AGAP007534 AAEL007339 AAEL005890 AAEL005884 AAEL005756 PHUM409080 GLEAN_13829
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
Bloomington	12683 w1118; P{GT1}Hsp22BG02409 Hsp67BbBG02409 20055 y1 w67c23; P{EPgy2}Hsp22EY09909 Hsp67BbEY09909
Genomic Clones ( 1 )
	BACR01E20 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 17 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD36162 LP05734
Other clones	DPiM_LD36162
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD16753 LD40634
Other clones	57630 73207 527120 bs75b08 EC22858 EC29258 EK178661 EK202215 EN07317 FGM209E07 FGM214B11 LD16746
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0001228 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 3771872
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments	Dicistronic annotation CG32041 split out into separate annotations for each open reading frame, CG4456 and CG4460, in release 4.2 of the genome annotation. CG4456 corresponds to Hsp67Bb and CG4460 corresponds to Hsp22. (FlyBase Genome Annotators, 2005) One or more of the processed transcripts for this gene contain(s) two non-overlapping open reading frames (ORFs). The non-overlapping ORFs are represented by Hsp67Bb and Hsp22. (FlyBase Genome Annotators, 2002-2003)
Other Comments
	In unshocked cells Hsp83 is moderately transcribed while transcription from the other heat shock genes is undetectable. Engaged but paused RNA molecules are found at the various Hsp70 and Hsp26 genes but not at the other heat shock genes. Increased transcription of the heat shock genes is observed within 1-2 mins of heat shock and maximal rates were reached within 2-5 minutes. Rates of transcription vary over a 20-fold range. (Vazquez et al., 1993) Exposure of cells to pulses of elevated temperature initiates the heat-shock response. A restricted subset of genes, the Hsp genes, is activated and the majority of transcription and translation is shut down. 3H-uridine incorporation ceases at its usual positions and commences at new puff sites. Preexisting polysomes disaggregate and within a few minutes a new population of polysomes appears containing newly transcribed mRNA; this RNA hybridizes to some of the heat-shock puffs. Similar response inducible by other stressful treatments. The response may be elicited at all stages of the life cycle and in cultured cells. (Lindsley and Zimm, 1992) Heat shock induces the accumulation of a dicistronic mRNA which contains both the Hsp22 and Hsp67Bb open reading frames. Overall orientation not stated: Hsp27- Hsp23- Hsp67Ba+ Hsp26+ Hsp22- Hsp67Bb- Hsp67Bc+ (Pauli et al., 1988) The binding sites for the protein factors required for activation of transcription of Hsp genes are multiple short upstream sequence elements called HSEs or heat shock consensus elements. (Xiao and Lis, 1988, Pelham, 1982) Hsp67Bb shows weak homology to Hsp22. It is heat inducible. (Ayme-Southgate and Tissieres, 1985) Activation of transcription of Hsp genes apparently involves the sequential binding of two or more protein factors in vicinity of TATA box. (Wu, 1984) Polymerase II dissociates from most chromosome regions and accumulates at the new heat shock puff sites upon heat shock. (Bonner and Kerby, 1982) Hsp67Bb is transcribed during certain developmental stages in the absence of heat shock. (Sirotkin and Davidson, 1982) The effects of heat shock may be abrogated to some degree by pretreatment with a pulse of a slightly lower temperature. (Mitchell et al., 1979, Petersen and Mitchell, 1981) Mitochondrial and histone-gene activities persist transcription and translation. (Spradling et al., 1977) In polytene cells, during heat shock response, existing puffs regress and a novel group quickly appear at 33B, 63C, 64F, 67B, 70A, 87A, 87C, 93D, 95D. (Tissieres et al., 1974) The heat shock response follows a pulse of 36oC to 40oC; treatments above 40oC inhibit all activity and lead to death; treatments of 30oC-35oC induce heat-shock-protein synthesis without repressing normal protein synthesis. (Tissieres et al., 1974) In polytene cells, during heat shock response, existing puffs regress and a novel group quickly appear at cytological locations 33B, 63C, 64F, 67B, 70A, 87A, 87C, 93D, 95D. (Ashburner, 1970)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_001031945 NM_001031946 NM_001202147 NM_001202148 RefSeq (Proteins) • NP_001027116 NP_001027117 NP_001189076 NP_001189077 DDBJ / EMBL / GenBank • AAF50290.1 AAF50291 AAF50291.1 AAN11961 AAN11961.1 AAN11962.1 ADV37513 ADV37514 AI456272 AQ073854 AW943326 AX093926 AX154789 AY060412 CAA30276 CL610262 X07311 Entrez Gene - A searchable database of RefSeq genes. • 3771872 UniProtKB/Swiss-Prot • P22978
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Rhodanese-like (IPR001763)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0001228 DRSC - Results from RNAi screens. • FBgn0001228 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0001228 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32041-RB;CG32041-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0001228 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 3771872 InParanoid A subset of ortholog calls from InParanoid. • 83482 Branchiostoma floridae (lancelet) ENSCAFP00000018657 Canis familiaris (dog) 144137 Chlamidomonas reinhardtii (green alga) 92990 Daphnia pulex (daphnia) ENSECAP00000021813 Equus caballus (horse) ENSGALP00000024896 Gallus gallus (chicken) ISCW023160-PA Ixodes scapularis (deer tick) LmjF09.1470 Leishmania major (virus) 9436 Monocosta brevicollis (choanoflagellate) 14863 Ostreococcus tauri (green alga) YOR285W Saccharomyces cerevisiae (budding yeast) YOR286W Saccharomyces cerevisiae (budding yeast) Smp_007450.2 Schistosoma mansoni (trematode parasite) Tc00.1047053506753.130 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053510357.20 Trypanosoma cruzi (parasitic trypanosome) 260962 Thalassiosira pseudonana (marine diatom) 39141 Thalassiosira pseudonana (marine diatom) YALI0B01650g Yarrowia lipolytica (nonconventional yeast) YALI0F29667g Yarrowia lipolytica (nonconventional yeast) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 3771872 modMine - Data generated by the modENCODE project. • FBgn0001228 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB17694 NV15588 LH19518 ISCW023160 ISCW015859 FBgn0243252 FBgn0240626 FBgn0068141 FBgn0219633 FBgn0215740 FBgn0213162 FBgn0201616 FBgn0201410 FBgn0198733 FBgn0192515 FBgn0190371 FBgn0185860 FBgn0181407 FBgn0180000 FBgn0178927 JGI_V11_92990 JGI_V11_219795 JGI_V11_336777 FBgn0248612 FBgn0245688 FBgn0071580 FBgn0162014 FBgn0160987 FBgn0160032 FBgn0147355 FBgn0135000 FBgn0133568 FBgn0039145 FBgn0038080 FBgn0132701 FBgn0126063 FBgn0125955 FBgn0122061 FBgn0111420 FBgn0107618 FBgn0103551 FBgn0097135 FBgn0094138 FBgn0094056 FBgn0087729 CPIJ013882 CPIJ013880 CPIJ013881 BGIBMGA002501 ACYPI008513 GB17201 AGAP013228 AGAP007534 AAEL007339 AAEL005890 AAEL005884 AAEL005756 PHUM409080 GLEAN_13829
Synonyms & Secondary IDs ( 18 )
Reported As
Symbol Synonym	anon-WO0118547.50   anon-WO0140519.138   CG4456   CG32041 (Giot et al., 2003, Birch-Machin et al., 2005) EP(3)3247 (Pena-Rangel et al., 2002) gene2 (Southgate et al., 1985) gene 2 (Vazquez et al., 1993, Vazquez, 1991, Pauli et al., 1989, Pauli et al., 1988, Ayme-Southgate and Tissieres, 1985) gene-2   gene4 (Sirotkin and Davidson, 1982) hsp67B (Ringrose et al., 2003) Hsp67Bb (Sudi et al., 2008, Yepiskoposyan et al., 2006, Bettencourt et al., 2008, Takahashi et al., 2010, Mosqueira et al., 2010) hsp67Bb (Morrow et al., 2004) Hspg2 (Maroni, 1994) Hsp-G2   small hsp locus 67B (Yao et al., 2007)
Name Synonym	Gene 2 (Pauli and Tonka, 1987) heat shock gene 2 (Pauli, 1997.11.7) Heat shock gene 67Bb
Secondary FlyBase IDs
	FBgn0044743 FBgn0052041 FBgn0061943 FBpp0076176 FBpp0076177 FBpp0076178 FBpp0076179 FBtr0076448 FBtr0076449 FBtr0076450 FBtr0076451
References ( 42 )
Generate a list of	All references relating to this gene ( 42 )
List References by type	Research paper  ( 29 ) Supplementary material  ( 1 ) FlyBase analysis  ( 4 ) DNA/RNA sequence record  ( 1 ) Patent  ( 2 ) Book  ( 1 ) Computer file  ( 2 ) Curated genome annotation  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers ( 2 )
	Mosqueira et al., 2010, PLoS ONE 5(10): e13450 Chronic Hypoxia Impairs Muscle Function in the Drosophila Model of Duchenne's Muscular Dystrophy (DMD). [FBrf0212137] Takahashi et al., 2010, BMC Evol. Biol. 10: 284 Effects of small Hsp genes on developmental stability and microenvironmental canalization. [FBrf0211941] Show all research papers ...